CN105543139A - Bacillus amyloliquefaciens for effectively degrading nitrite - Google Patents
Bacillus amyloliquefaciens for effectively degrading nitrite Download PDFInfo
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- CN105543139A CN105543139A CN201610019210.4A CN201610019210A CN105543139A CN 105543139 A CN105543139 A CN 105543139A CN 201610019210 A CN201610019210 A CN 201610019210A CN 105543139 A CN105543139 A CN 105543139A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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Abstract
The invention relates to environment treatment with microorganisms, belongs to the technical field of bioengineering and particularly relates to a strain of bacillus amyloliquefaciens capable of conducting metabolism on nitrite. The strain number of the bacillus amyloliquefaciens capable of conducting metabolism on nitrite is H47, the strain was preserved in China Center for Type Culture Collection on Jun 24th, 2013 with the preservation serial number being CCTCC M 2013283. The bacillus amyloliquefaciens capable of conducting metabolism on nitrite can effectively degrade nitrite, the strain can be utilized to develop corresponding environmental biological agents, and the strain has good application prospect.
Description
Technical field
The present invention relates to and utilize microorganism to carry out environmental improvement aspect, belong to technical field of bioengineering.Be specifically related to the bacillus amyloliquefaciens of a strain degradable nitrite.
Background technology
Nitrite is applied to Industry and Construction as oxygenant, also can be used as chromogenic reagent and sanitas limitation be applied to meat product, but nitrite can cause baby's methemoglobinemia, baby is in congenital malformation, and thyrocele is also reacted with protein breakdown products secondary amine and generated strong carcinogen nitrosamine.Water body and nitrite in food finite quantity standard.
In culture fishery, along with improving constantly of cultivation level, continuing to increase of cultivation density, also constantly increasing the input in pond, the load of water body mostly meets or exceeds degree of saturation, and then the physico chemical factor of water body is constantly worsened.The hazardous and noxious substances such as the ammonia nitrogen in water body, nitrite nitrogen (nitrite) produce in a large number, cause cultured fishes to be easily poisoned to death, often bring more heavy loss to raiser.
Microbial degradation method is considered to the effective ways reducing or eliminating hazardous compound pollution, and the food safety microorganism screening effective degrading nitrite is the effective ways realizing reducing or eliminating water body and leavened food nitrite.
Summary of the invention:
The object of this invention is to provide a strain can the bacterial strain-bacillus amyloliquefaciens of effective degrading nitrite.(BacillusamyloliquefaciensH47)。
Degradation bacteria strains provided by the present invention is that bacillus amyloliquefaciens (BacillusamyloliquefaciensH47) is preserved in China typical culture collection center, Luo Jia Shan, wuchang, wuhan, preservation place Wuhan University, deposit number is CCTCCM2013283, preservation date on June 24th, 2013.
The step of described bacillus amyloliquefaciens degrading nitrite is: the 1) preparation of bacterial strain H47 seed liquor.Go bail for be hidden in-80 DEG C of glycerine pipes seed liquor 50 μ L in being equipped with in 50mLLB substratum 250mL triangular flask, cultivate in 30 DEG C of 200r/min shaking tables.
2) mensuration of bacterial strain H47 degrading nitrite: accurately pipette 0.5mL uniform activation seed liquor in the 250mL triangular flask that 50mLLB substratum (containing 150mg/LNaNO2) is housed.30 DEG C of cultivations, timing sampling.Obtaining fermented liquid supernatant, according to method described in GB5009.33-2,010 2---spectrophotometry (slightly changing) measures fermented liquid nitrite.
3) described fermented liquid supernatant preparation method is that fermented liquid is centrifugal under 8000r/min whizzer, obtains supernatant.
4) method of described spectrophotometry fermented liquid nitrite is: get 3mL supernatant liquor and add deionized water to 28.5mL, add ferrous nitrilation potassium (106g/L) 0.75mL, acetic acid zinc solution (106g/L) 0.75mL, mixing reaction 30min.The centrifugal 10min of 8000r/min.Get 20mL supernatant in 25mL colorimetric cylinder, to add 2mL Sodium sulfanilate solution (4g/L) put upside down homogeneous reaction 3min and add 1mL hydrochloride naphthodiamide solution (2g/L) again, 25mL is settled to deionized water, put upside down evenly, reaction 15min, surveys absorbancy in wavelength 538nm place.
The present invention's substratum used is LB substratum: Tryptones 10g, yeast extractive substance 5g, and NaCl10g, adds water and be settled to 1L, adjusts pH to be 7.1 ± 0.1,115 DEG C of sterilizing 20min.
Compared with other technologies, the positively effect of this invention is: bacillus amyloliquefaciens is generally acknowledged Biosafety bacterium, nontoxic to people and animals, free from environmental pollution.Its meta-bolites comparatively horn of plenty, has broad spectrum antibiotic activity and stronger anti-adversity ability, and growth is fast, and stability is better.This bacterium degradable nitrite, has speed fast, the advantages such as expense is low, and operation steps is simple, provides good bacterium source and thinking for reducing Nitrite content.
Accompanying drawing illustrates:
Fig. 1 is the degradation curve that degradation bacteria strains H47 acts on the Sodium Nitrite of 150mg/L.
Fig. 2 is the degradation capability of degradation bacteria strains H47 to different concns Sodium Nitrite.
Embodiment:
Below in conjunction with embodiment, the present invention is described further.Following examples are only several specific embodiment of the present invention, but design concept of the present invention is not limited thereto, and all changes utilizing this design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.
Embodiment 1: different incubation time is to the situation of bacterial strain H47 degrading nitrite.
The preparation of bacterial classification H47 seed liquor: go bail for be hidden in-80 DEG C of glycerine pipes seed liquor 50 μ L in being equipped with in 50mLLB substratum 250mL triangular flask, in 30 DEG C, 200r/min shaking table cultivate.
Accurately pipette 0.5mL uniform activation seed liquor in the 250mL triangular flask that 50mLLB substratum (containing 150mg/LNaNO2) is housed.30 DEG C of cultivations, timing sampling.Obtaining fermented liquid supernatant, according to method described in GB5009.33-2,010 2---spectrophotometry (slightly changing) measures fermented liquid nitrite.Blank is made with the LB substratum of the Sodium Nitrite containing 150mg/L not connecing H47 bacterial strain.Bacterial strain H47 acts on the Sodium Nitrite LB substratum containing 150mg/L, to the degradation curve of Sodium Nitrite as shown in Figure 1.Bacterial strain H47 has obvious Degradation to Sodium Nitrite, and 12h can reach 99.77% to the degradation rate of Sodium Nitrite.
Described fermented liquid supernatant preparation method is that fermented liquid is centrifugal under 8000r/min whizzer, obtains supernatant.
The method of described spectrophotometry fermented liquid nitrite is: get 3mL supernatant liquor and add deionized water to 28.5mL, add ferrous nitrilation potassium (106g/L) 0.75mL, acetic acid zinc solution (106g/L) 0.75mL, mixing reaction 30min.The centrifugal 10min of 8000r/min.Get 20mL supernatant in 25mL cuvette, to add 2mL Sodium sulfanilate solution (4g/L) put upside down homogeneous reaction 3min and add 1mL hydrochloride naphthodiamide solution (2g/L) again, 25mL is settled to deionized water, put upside down evenly, reaction 15min, surveys absorbancy in wavelength 538nm place.
The calculation formula of Sodium Nitrite degradation rate is:
Degradation rate (%)=(C – Cn) 100%/C
In formula: C is the concentration (mg/L) of blank nitrite, Cn is the concentration (mg/L) of fermented liquid nitrite.
Described substratum is: LB substratum: Tryptones 10g, yeast extractive substance 5g, and NaCl10g, adds water and be settled to 1L, adjusts pH to be 7.1 ± 0.1,115 DEG C of sterilizing 20min.
Example 2) degradation bacteria strains H47 situation that different concns Sodium Nitrite is degraded.
The preparation of bacterial classification H47 seed liquor: go bail for be hidden in-80 DEG C of glycerine pipes seed liquor 50 μ L in being equipped with in 50mLLB substratum 250mL triangular flask, in 30 DEG C, 200r/min shaking table cultivate.
Accurately pipette 0.5mL uniform activation seed liquor in the 250mL triangular flask that 50mLLB substratum (being respectively 50,100,150,200,250 containing Sodium Nitrite concentration (mg/L)) is housed.Cultivate 10h for 30 DEG C.Obtaining fermented liquid supernatant, according to method described in GB5009.33-2,010 2---spectrophotometry (slightly changing) measures fermented liquid nitrite.Blank is made with (being respectively 50,100,150,200,250 containing Sodium Nitrite concentration (mg/L)) the LB substratum not connecing H47 bacterial strain.Degradation bacteria strains H47 to the degraded situation of different concns Sodium Nitrite as shown in Figure 2, bacterial strain H47 all can grow and all have the effect of efficient degradation Sodium Nitrite in the Sodium Nitrite substratum of 50-250mg/L, and degradation efficiency first strengthens with the increase of Sodium Nitrite concentration and tends towards stability afterwards.
Described fermented liquid supernatant preparation method is that fermented liquid is centrifugal under 8000r/min whizzer, obtains supernatant.
The method of described spectrophotometry fermented liquid nitrite is: get 3mL supernatant liquor and add deionized water to 28.5mL, add ferrous nitrilation potassium (106g/L) 0.75mL, acetic acid zinc solution (106g/L) 0.75mL, mixing reaction 30min.The centrifugal 10min of 8000r/min.Get 20mL supernatant in 25mL cuvette, to add 2mL Sodium sulfanilate solution (4g/L) put upside down homogeneous reaction 3min and add 1mL hydrochloride naphthodiamide solution (2g/L) again, 25mL is settled to deionized water, put upside down evenly, reaction 15min, surveys absorbancy in wavelength 538nm place.
The calculation formula of Sodium Nitrite degradation rate is:
Degradation rate (%)=(C – Cn) 100%/C
In formula: C is the concentration (mg/L) of blank nitrite, Cn is the concentration (mg/L) of fermented liquid nitrite.
Described substratum is: LB substratum: Tryptones 10g, yeast extractive substance 5g, and NaCl10g, adds water and be settled to 1L, adjusts pH to be 7.1 ± 0.1,115 DEG C of sterilizing 20min.
Claims (2)
1. a bacillus amyloliquefaciens (BacillusamyloliquefaciensH47) is characterized in that: be preserved in China typical culture collection center, Luo Jia Shan, wuchang, wuhan, preservation place Wuhan University, deposit number is CCTCCM2013283, preservation date on June 24th, 2013.
2. it is characterized in that can effective degrading nitrite for bacillus amyloliquefaciens described in claim 1 (BacillusamyloliquefaciensH47), for the degraded of water source and leavened food nitrite and elimination provide good bacterium source.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113249270A (en) * | 2021-06-28 | 2021-08-13 | 华南师范大学 | Salt-resistant high-self-aggregation-capability bacillus amyloliquefaciens and application thereof in denitrification |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060389A (en) * | 2010-11-10 | 2011-05-18 | 天津市农业生物技术研究中心 | Composite microecological preparation capable of degrading nitrites in water body rapidly |
| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
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- 2016-01-12 CN CN201610019210.4A patent/CN105543139A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102060389A (en) * | 2010-11-10 | 2011-05-18 | 天津市农业生物技术研究中心 | Composite microecological preparation capable of degrading nitrites in water body rapidly |
| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
Non-Patent Citations (4)
| Title |
|---|
| FENGXING XIE ET AL: "Using Bacillus amyloliquefaciens for remediation of aquaculture water", 《SPRINGERPLUS》 * |
| 曹海鹏 等: "具有降解亚硝酸盐活性的解淀粉芽孢杆菌的分离与安全性分析", 《2012全国水工泄水建筑物安全与病害处理技术应用会刊》 * |
| 曹海鹏 等: "具有降解亚硝酸盐活性的解淀粉芽孢杆菌的分离与安全性分析", 《环境污染与防治》 * |
| 谢凤行 等: "一株解淀粉芽孢杆菌的分离鉴定及水质净化效果的研究", 《环境科学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113249270A (en) * | 2021-06-28 | 2021-08-13 | 华南师范大学 | Salt-resistant high-self-aggregation-capability bacillus amyloliquefaciens and application thereof in denitrification |
| CN113249270B (en) * | 2021-06-28 | 2021-11-26 | 华南师范大学 | Salt-resistant high-self-aggregation-capability bacillus amyloliquefaciens and application thereof in denitrification |
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Application publication date: 20160504 |