CN103468621B - Compound microbial preparation for purifying water body - Google Patents
Compound microbial preparation for purifying water body Download PDFInfo
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Abstract
The invention aims to provide a compound microbial preparation which consists of bacillus cereus, bacillus licheniformis, halophilic pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium, wherein the viable count is as high as 8 multiplied by 1011CFU/g, can be widely applied to fresh water and mariculture water areas. The synergistic effect among the strains can effectively degrade ammonia nitrogen and nitrite in the water body and inhibit the generation of water bloom, thereby purifying the aquaculture water body, improving the growth environment of the cultured animals and improving the production performance.
Description
Technical Field
The invention relates to the technical field of microorganism application, in particular to a compound microbial preparation for purifying a water body.
Background
In a natural state, the indigenous bacteria in the water body can maintain normal material circulation of the water body through a self-purification effect. However, with the development of high-density aquaculture, the organic load in the aquaculture water body greatly exceeds the self-purification capacity of the original flora. Organic matters which are not effectively utilized become the largest pollution source in a water body, a large amount of dissolved oxygen is consumed, oxygen debt in the water body is caused, further, complete oxidation of the organic matters is inhibited, a large amount of incomplete oxidation intermediates are formed, incomplete oxidation products are often toxic substances which are most harmful in aquaculture, such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, chronic and acute poisoning of cultured animals is caused, large-scale dead fishes are seriously caused, and economic losses are disastrous.
In recent years, microbial preparations have come to be widely applied to the field of cultivation, and are used for purifying water, increasing feed intake of cultivated animals, reducing occurrence of diseases and the like. Beneficial microorganisms put into water bodies are grown and bred in large quantities, and secrete rich extracellular enzymes to effectively decompose organic matters, and the microorganisms can utilize ammonia nitrogen, metabolize nitrite, oxidize hydrogen sulfide and the like in the growth and metabolism process, so that the aims of degrading toxic substances, purifying water quality and improving cultivation benefits are fulfilled.
At present, the commonly used microbial preparation for purifying water bodies mostly adopts a single bacterial strain or the composite action of two or three bacterial strains, the number of the live bacteria in the preparation is low, and the quality of the culture water body can not be comprehensively and effectively improved, so that the current urgent need is to actively develop a novel composite microbial preparation and comprehensively improve the current culture situation.
Disclosure of Invention
The invention provides a compound microbial preparation capable of purifying water for solving the problems of the prior art. The preparation has the functions of obviously adjusting water color, reducing ammonia nitrogen and nitrite, improving transparency, promoting the growth of cultured animals and the like through the synergistic promotion effect of various microorganisms.
The applicant discovers through long-term screening research that the bacillus cereus, the bacillus licheniformis, the halophilic pseudomonas, the lactobacillus acidophilus, the rhodococcus erythropolis, the rhodopseudomonas palustris and the enterococcus faecium can generate a synergistic effect, effectively degrade ammonia nitrogen and nitrite in water, and improve water quality, thereby promoting the invention.
In one aspect, the invention provides a composite microbial preparation comprising Bacillus cereus (Bacillus cereus) (II)Bacillus cereus) Bacillus licheniformis (B), (B)Bacillus licheniformis) Pseudomonas halophilus (A)Halomonas sulfidaeris) Lactobacillus acidophilus (a)Lactobacillus acidophilus) Rhodococcus erythropolis (C.erythropolis)Rhodococcus erythropolis) Rhodopseudomonas palustris (a)Rhodopseudomonas palustris) And enterococcus faecium (A), (B), (C)Enterococcus faecium) And (4) forming.
The compound microbial preparation preferably contains Bacillus cereus CGMCC1.10163, Bacillus licheniformis CGMCC1.10257, Pseudomonas halophilus CCTCC M2013070, Lactobacillus acidophilus CGMCC1.3915, Rhodococcus erythropolis CGMCC1.3613, Rhodopseudomonas palustris CGMCC1.2352, and enterococcus faecium CGMCC 1.2334.
The composite microbial preparation comprises the following components in percentage by weight: 20-50 parts of bacillus cereus, 10-30 parts of bacillus licheniformis, 5-15 parts of halophilic pseudomonas, 1-5 parts of lactobacillus acidophilus, 1-5 parts of rhodococcus erythropolis, 10-15 parts of rhodopseudomonas palustris and 10-30 parts of enterococcus faecium.
The content of each component in the composite microbial preparation is preferably as follows: 40 parts of bacillus cereus, 25 parts of bacillus licheniformis, 10 parts of halophilic pseudomonas, 3 parts of lactobacillus acidophilus, 3 parts of rhodococcus erythropolis, 12 parts of rhodopseudomonas palustris and 22 parts of enterococcus faecium.
The invention provides the application of the composite microbial preparation in water quality purification.
The dosage of the compound microbial preparation is 20-40 g/mu.m, and the compound microbial preparation is used once every other week.
In another aspect, the present invention provides a method for producing the above complex microbial preparation, comprising the steps of:
1) the bacillus cereus, bacillus licheniformis, halophilus pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium strains are respectively fermented by high-density liquid, and the spore content is up to 10 percent by a post-treatment technology of a fermentation process11-1012CFU/g of super concentrated bacterial powder;
2) preparing the hyperconcentration bacterial powder according to 20-50 parts of bacillus cereus, 10-30 parts of bacillus licheniformis, 5-15 parts of halophilic pseudomonas, 1-5 parts of lactobacillus acidophilus, 1-5 parts of rhodococcus erythropolis, 10-15 parts of rhodopseudomonas palustris and 10-30 parts of enterococcus faecium;
3) and packaging the prepared compound microbial preparation by adopting a water-soluble material.
Advantageous effects
The number of viable bacteria in the composite microbial preparation of the invention is as high as 8 multiplied by 1011CFU/g, can be widely applied to fresh water and mariculture water areas. The synergistic effect among the strains can effectively degrade ammonia nitrogen and nitrite in the water body and inhibit the generation of water bloom, thereby purifying the aquaculture water body, improving the growth environment of the cultured animals and improving the production performance. The production method provided by the invention is simple, is suitable for most of the existing fermentation processes and equipment, and is beneficial to large-scale popularization and application.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications can be made by those skilled in the art after reading the disclosure of the present invention, and equivalents fall within the scope of the appended claims.
The various reagents and devices related to the invention can be selected from any one of the reagents and devices which are commercially available; the invention relates to a bacillus cereus strain, which is purchased from China general microbiological preservation management center with the preservation number of 1.10163; the bacillus licheniformis is purchased from China general microbiological culture Collection center with the preservation number of 1.10257; the halophilic pseudomonas is a patent strain of Uloric blue biology group Limited company in Qingdao, and the preservation number is CCTCC M2013070; the lactobacillus acidophilus is purchased from China general microbiological culture Collection center with the preservation number 1.3915; rhodococcus erythropolis is purchased from China center for general microbiological collection and management, and is deposited with the number 1.3613; the rhodopseudomonas palustris is purchased from China general microbiological collection center with the collection number of 1.2352; enterococcus faecium is purchased from China general microbiological collection center with the collection number 1.2334.
Example 1
A compound microbial preparation comprises Bacillus cereus, Bacillus licheniformis, Pseudomonas halophilus, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium, and comprises the following components in percentage by weight: 20 parts of bacillus cereus, 30 parts of bacillus licheniformis, 15 parts of halophilic pseudomonas, 5 parts of lactobacillus acidophilus, 5 parts of rhodococcus erythropolis, 10 parts of rhodopseudomonas palustris and 30 parts of enterococcus faecium.
The production method of the compound microbial preparation comprises the following steps:
1) the bacillus cereus, bacillus licheniformis, halophilus pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium strains are respectively fermented by high-density liquid, and the spore content is up to 10 percent by a post-treatment technology of a fermentation process11-1012CFU/g of super concentrated bacterial powder;
2) preparing the hyperconcentration bacterial powder according to 20 parts of bacillus cereus, 30 parts of bacillus licheniformis, 15 parts of pseudomonas halophilus, 5 parts of lactobacillus acidophilus, 5 parts of rhodococcus erythropolis, 10 parts of rhodopseudomonas palustris and 30 parts of enterococcus faecium;
3) and packaging the prepared compound microbial preparation by adopting a water-soluble material.
Example 2
A compound microbial preparation comprises Bacillus cereus, Bacillus licheniformis, Pseudomonas halophilus, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium, and comprises the following components in percentage by weight: 50 parts of bacillus cereus, 10 parts of bacillus licheniformis, 5 parts of halophilic pseudomonas, 1 part of lactobacillus acidophilus, 1 part of rhodococcus erythropolis, 15 parts of rhodopseudomonas palustris and 10 parts of enterococcus faecium.
The production method of the compound microbial preparation comprises the following steps:
1) the bacillus cereus, bacillus licheniformis, halophilus pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium strains are respectively fermented by high-density liquid, and the spore content is up to 10 percent by a post-treatment technology of a fermentation process11-1012CFU/g of super concentrated bacterial powder;
2) preparing the hyperconcentration bacterial powder according to 50 parts of bacillus cereus, 10 parts of bacillus licheniformis, 5 parts of halophilic pseudomonas, 1 part of lactobacillus acidophilus, 1 part of rhodococcus erythropolis, 15 parts of rhodopseudomonas palustris and 10 parts of enterococcus faecium;
3) and packaging the prepared compound microbial preparation by adopting a water-soluble material.
Example 3
A compound microbial preparation comprises Bacillus cereus, Bacillus licheniformis, Pseudomonas halophilus, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium, and comprises the following components in percentage by weight: 40 parts of bacillus cereus, 25 parts of bacillus licheniformis, 10 parts of halophilic pseudomonas, 3 parts of lactobacillus acidophilus, 3 parts of rhodococcus erythropolis, 12 parts of rhodopseudomonas palustris and 22 parts of enterococcus faecium.
The production method of the compound microbial preparation comprises the following steps:
1) mixing the above Bacillus cereus, Bacillus licheniformis and Pseudomonas halophilusLactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium strains are respectively fermented by high-density liquid, and the spore content is up to 1011-1012CFU/g of super concentrated bacterial powder;
2) preparing the hyperconcentration bacterial powder according to 40 parts of bacillus cereus, 25 parts of bacillus licheniformis, 10 parts of halophilic pseudomonas, 3 parts of lactobacillus acidophilus, 3 parts of rhodococcus erythropolis, 12 parts of rhodopseudomonas palustris and 22 parts of enterococcus faecium;
3) and packaging the prepared compound microbial preparation by adopting a water-soluble material.
Example 4
The composite microbial preparations prepared in examples 1 to 3 were subjected to viable cell count, and the results are shown in table 1:
TABLE 1 comparison of viable count in composite microbial preparations
| Sample (I) | Number of viable bacteria |
| Example 1 | 5×1011CFU/g |
| Example 2 | 2×1011CFU/g |
| Example 3 | 8×1011CFU/g |
Example 5 application of Compound microbial preparation in Paralichthys olivaceus culture Water
Selecting two paralichthys olivaceus culture ponds with similar water body environmental characteristics, wherein the water area is 1 mu, the water depth is about 1.5 m, and the ponds are divided into a control group and a test group. The control group is not treated, the compound microbial preparation prepared by the embodiment of the invention is directly thrown to the vicinity of an aerator according to the dosage of 25 g/mu.m in the test group, and the aerator is started for 1 hour, so that the microbial preparation is fully dispersed, and the action effect is more full. And (3) respectively taking water samples of a control group and a test group at the same time every other day for water quality detection for 7 days continuously, wherein the results are shown in Table 2.
TABLE 2 Paralichthys olivaceus culture water area Water quality test results
"-" indicates no detection.
As can be seen from Table 2, the compound microbial preparation of the invention can significantly improve the transparency of the water body of the aquaculture water area, degrade harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide, reduce environmental pollution, provide a healthy growth environment for aquaculture animals and improve the production performance of the aquaculture animals.
Claims (4)
1. A compound microbial preparation comprises Bacillus cereus CGMCC1.10163, Bacillus licheniformis CGMCC1.10257, Pseudomonas halophilus CCTCC M2013070, Lactobacillus acidophilus CGMCC1.3915, Rhodococcus erythropolis CGMCC1.3613, Rhodopseudomonas palustris CGMCC1.2352 and enterococcus faecium CGMCC1.2334, wherein the contents of the components are respectively as follows: 20-50 parts of bacillus cereus, 10-30 parts of bacillus licheniformis, 5-15 parts of halophilic pseudomonas, 1-5 parts of lactobacillus acidophilus, 1-5 parts of rhodococcus erythropolis, 10-15 parts of rhodopseudomonas palustris and 10-30 parts of enterococcus faecium.
2. The compound microbial preparation according to claim 1, wherein the contents of the components are respectively as follows: 40 parts of bacillus cereus, 25 parts of bacillus licheniformis, 10 parts of halophilic pseudomonas, 3 parts of lactobacillus acidophilus, 3 parts of rhodococcus erythropolis, 12 parts of rhodopseudomonas palustris and 22 parts of enterococcus faecium.
3. Use of the complex microbial preparation of claim 1 for water purification.
4. The use of the complex microbial preparation of claim 3, wherein the complex microbial preparation is used in an amount of 20 to 40 g/mu-m once every 7 days.
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| CN104738362B (en) * | 2013-12-27 | 2019-08-02 | 中粮营养健康研究院有限公司 | Purposes of the red city Rhodococcus sp in the aflatoxin B1 in degradation brewing seasonings or its raw material |
| CN104045166A (en) * | 2014-06-23 | 2014-09-17 | 河南大学 | Compound microorganism bacterium agent and application thereof in eutrophic water body treatment |
| CN104512968A (en) * | 2014-10-20 | 2015-04-15 | 镇江拜因诺生物科技有限公司 | Mariculture water body purification composite microbial preparation |
| CN104611279B (en) * | 2015-03-02 | 2017-06-09 | 中蓝连海设计研究院 | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes |
| CN106107022A (en) * | 2016-07-06 | 2016-11-16 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | A kind of suppression escherichia coli compound microbial preparation used for aquiculture |
| CN106186560A (en) * | 2016-08-29 | 2016-12-07 | 浙江恒生印染有限公司 | A kind of energy-saving and emission-reducing sewage water treatment method |
| CN106754559A (en) * | 2017-02-06 | 2017-05-31 | 青岛农业大学 | A kind of probiotics preparation method for effectively purifying water |
| CN108865935A (en) * | 2018-07-05 | 2018-11-23 | 济宁滨阳生物科技股份有限公司 | A kind of probiotics of water quality improvement and preparation method thereof |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
| CN112322539B (en) * | 2020-11-14 | 2022-05-20 | 江苏海洋大学 | Enterococcus faecium R-NTR-1 from ocean and screening method and application thereof |
| CN114657081A (en) * | 2020-12-22 | 2022-06-24 | 湖北绿天地生物科技有限公司 | Ternary composite bacterium preparation and application thereof |
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