CN104512968A - Mariculture water body purification composite microbial preparation - Google Patents

Mariculture water body purification composite microbial preparation Download PDF

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Publication number
CN104512968A
CN104512968A CN201410556088.5A CN201410556088A CN104512968A CN 104512968 A CN104512968 A CN 104512968A CN 201410556088 A CN201410556088 A CN 201410556088A CN 104512968 A CN104512968 A CN 104512968A
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CN
China
Prior art keywords
complex microorganism
water body
microorganism preparations
bacillus
pseudomonas
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Pending
Application number
CN201410556088.5A
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Chinese (zh)
Inventor
朱道辰
谢长校
陈焱
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ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
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ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
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Priority to CN201410556088.5A priority Critical patent/CN104512968A/en
Publication of CN104512968A publication Critical patent/CN104512968A/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/08—Seawater, e.g. for desalination
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/22—Eliminating or preventing deposits, scale removal, scale prevention
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment

Abstract

The invention discloses a mariculture water body purification composite microbial preparation, and belongs to the technical field of water body purification. The contents of component concentrated bacterial powders of the composite microbial preparation comprise 20-50% of bacillus subtilis, 5-15% of bacillus licheniformis, 10-30% of bacillus cereus, 1-5% of pseudomonas aeruginosa, 1-5% of rhodococcus erythropolis, 5-10% of pseudomonashalophila, 5%-15% of rhodopseudomonas palustris, and 15%-40% of enterococcus faecium. After the bacterial powders are mixed in proportion, packaging is carried out by adopting a water soluble material. The composite microbial preparation is put into a mariculture water body, enables dominant strains to be reproduced rapidly, allows culture pond redundant remnant feeds, feces, animal and plant dead bodies and other organic pollutants to be decomposed by active and abundant enzyme systems, and can significantly reduce the contents of ammonia nitrogen and nitrite in the culture water body.

Description

A kind of marine culture water purification complex microorganism preparations
Technical field
The present invention relates to a kind of water body purification technology, particularly relate to a kind of microbial compound preparation for marine culture water purification.
Background technology
At present due to the development of marine resources development and utilization, sea farming occupies more and more consequence in China's aquaculture.But also due to the fast development of sea farming, water resource quality declines, and paralic environment pollutes aggravation, especially high-density, intensive culture.Excessive input bait, excessively uses chemicals, cultivates excremental discharge etc., and the excessive and levels of toxic substances such as nitrite, hydrogen sulfide of nitrogen in seawater, phosphorus nutrition thing is raised greatly.It is even dead that the seawater eutrophication that the increasing of these substances content causes injure certainly will to again cultivation biozone on a large scale, carrys out massive losses to while harm marine ecology cultivation industrial zone.Therefore, water pollution issue is also the bottleneck problem that sea farming runs into.
Utilize microorganism to improve effective way that water quality is an economic environmental protection, microbial preparation is also more and more extensive in the utilization in cultivation field in recent years.When organic loading in water body exceedes the microorganism self-purification capacity of itself, drop into appropriate beneficial microorganism to water body, the objectionable impurities in degraded water body and decomposition can be helped to utilize unnecessary nutrition, prevent body eutrophication, thus improve water quality, improve the economic benefit of aquaculture.
The current microbial preparation applying to sea farming mostly in a large number is single bacterial strain, or the simple composite preparation of two or three kind of bacterial strain, and the content of its viable bacteria is often unsatisfactory.And applicant finds by the synergy of multiple bacterial strain, to produce the purifying water effect being significantly higher than single bacterial strain by great many of experiments and observational study.
Summary of the invention
The present invention be directed to too single for the microorganism water purification preparation bacterial strain of sea farming at present, viable count is on the low side, can not meet the problems such as aquaculture water purification demand comprehensively.Object is to provide one group of bacterial strain, utilizes its synergy each other, greatly improves cleaning water.Viable count is high, cheap, and consumption is few, easy to operate, Be very effective.
Applicant of the present invention, by long-term screening and research, finds one group can reach the composite bacteria of remarkable purifying water effect by collaborative promoter action.This group bacterial strain comprises: subtilis, Bacillus licheniformis, wax genus bacillus, Pseudomonas aeruginosa, red city rhodococcus, addicted to salt pseudomonas, Rhodopseudomonas palustris and faecium.The composite fungus agent be made up of this group bacterial strain is put in marine culture water, can breed rapidly as dominant strain, and residual bait unnecessary for culturing pool, movement, animals and plants corpse and other organic pollutants decompose by the enzyme system active and abundant with it, significantly can reduce ammonia nitrogen, the nitrite content in aquaculture water.
The present invention's preferred microbial compound preparation component: subtilis CGMCCNo.2288, Bacillus licheniformis CGMCC1.10257, wax genus bacillus CGMCC1.10163, Pseudomonas aeruginosa CGMCC0190, red city rhodococcus CGMCC1.3613, addicted to salt pseudomonas CCTCC M 2013070, Rhodopseudomonas palustris CGMCC1.2352 and faecium CGMCC1.2334.
The each component of mentioned microorganism compound formulation is all fermented by high density liquid, by zymotechnique post-processing technology, makes spore content up to 10 10-10 12the hyperconcentration bacterium powder of CFU/g.
The consumption that each component of complex microorganism preparations of the present invention concentrates bacterium powder is: subtilis 20%-50%, Bacillus licheniformis 5%-15%, bacillus cereus 10%-30%, Pseudomonas aeruginosa 1%-5%, red city rhodococcus 1%-5%, bite salt pseudomonas 5%-10%, Rhodopseudomonas palustris 5%-15%, faecium 15%-40%.
By above-mentioned system bacterium powder mix by a certain percentage after, adopt water-soluble material pack.
Embodiment
The each component of complex microorganism preparations of the present invention all adopts high density liquid to ferment, and substratum agents useful for same and material all come from commercially available any one.The control of culture medium prescription and the condition such as physics and chemistry, temperature is all with reference to the general zymotechnique of related microorganisms.
Each component ratio involved in the present invention as a reference, is not restricted to the scope of the invention.Person skilled can be revised as the case may be and flexibly, is specifically described the present invention by following embodiment.
Embodiment 1
A kind of complex microorganism preparations, by subtilis, Bacillus licheniformis, wax genus bacillus, Pseudomonas aeruginosa, red city rhodococcus, forms addicted to salt pseudomonas, Rhodopseudomonas palustris and faecium.Wherein the content of individual component is: subtilis 30%, Bacillus licheniformis 10%, bacillus cereus 20%, Pseudomonas aeruginosa 3%, red city rhodococcus 2%, addicted to salt pseudomonas 5%, Rhodopseudomonas palustris 10% and faecium 20%.
Above-mentioned each microorganism is fermented respectively by high density liquid, by zymotechnique post-processing technology, makes spore content up to 10 10-10 12the hyperconcentration bacterium powder of CFU/g.Then by each hyperconcentration bacterium powder according to following ratio mixed preparing: subtilis 30%, Bacillus licheniformis 10%, bacillus cereus 20%, Pseudomonas aeruginosa 3%, red city rhodococcus 2%, addicted to salt pseudomonas 5%, Rhodopseudomonas palustris 10% and faecium 20%.
The compound formulation water-soluble material prepared is packed.
Embodiment 2
A kind of complex microorganism preparations, by subtilis, Bacillus licheniformis, wax genus bacillus, Pseudomonas aeruginosa, red city rhodococcus, forms addicted to salt pseudomonas, Rhodopseudomonas palustris and faecium.Wherein the content of individual component is: subtilis 35%, Bacillus licheniformis 10%, bacillus cereus 10%, Pseudomonas aeruginosa 2%, red city rhodococcus 5%, addicted to salt pseudomonas 8%, Rhodopseudomonas palustris 10% and faecium 20%.
Above-mentioned each microorganism is fermented respectively by high density liquid, by zymotechnique post-processing technology, makes spore content up to 10 10-10 12the hyperconcentration bacterium powder of CFU/g.Then by each hyperconcentration bacterium powder according to following ratio mixed preparing: subtilis 35%, Bacillus licheniformis 10%, bacillus cereus 10%, Pseudomonas aeruginosa 2%, red city rhodococcus 5%, addicted to salt pseudomonas 8%, Rhodopseudomonas palustris 10% and faecium 20%.
The compound formulation water-soluble material prepared is packed.
Embodiment 3
A kind of complex microorganism preparations, by subtilis, Bacillus licheniformis, wax genus bacillus, Pseudomonas aeruginosa, red city rhodococcus, forms addicted to salt pseudomonas, Rhodopseudomonas palustris and faecium.Wherein the content of individual component is: subtilis 20%, Bacillus licheniformis 10%, bacillus cereus 20%, Pseudomonas aeruginosa 3%, red city rhodococcus 2%, addicted to salt pseudomonas 10%, Rhodopseudomonas palustris 10% and faecium 25%.
Above-mentioned each microorganism is fermented respectively by high density liquid, by zymotechnique post-processing technology, makes spore content up to 10 10-10 12the hyperconcentration bacterium powder of CFU/g.Then by each hyperconcentration bacterium powder according to following ratio mixed preparing: subtilis 20%, Bacillus licheniformis 10%, bacillus cereus 20%, Pseudomonas aeruginosa 3%, red city rhodococcus 2%, addicted to salt pseudomonas 10%, Rhodopseudomonas palustris 10% and faecium 25%.
The compound formulation water-soluble material prepared is packed.
Embodiment 4
A kind of complex microorganism preparations, by subtilis, Bacillus licheniformis, wax genus bacillus, Pseudomonas aeruginosa, red city rhodococcus, forms addicted to salt pseudomonas, Rhodopseudomonas palustris and faecium.Wherein the content of individual component is: subtilis 40%, Bacillus licheniformis 5%, bacillus cereus 5%, Pseudomonas aeruginosa 5%, red city rhodococcus 5%, addicted to salt pseudomonas 5%, Rhodopseudomonas palustris 5% and faecium 30%.
Above-mentioned each microorganism is fermented respectively by high density liquid, by zymotechnique post-processing technology, makes spore content up to 10 10-10 12the hyperconcentration bacterium powder of CFU/g.Then by each hyperconcentration bacterium powder according to following ratio mixed preparing: subtilis 40%, Bacillus licheniformis 5%, bacillus cereus 5%, Pseudomonas aeruginosa 5%, red city rhodococcus 5%, addicted to salt pseudomonas 5%, Rhodopseudomonas palustris 5% and faecium 30%.
The compound formulation water-soluble material prepared is packed.
Embodiment 5
In same seawater fishery factory, select the shrimp culture pond that four water body environments are similar, be numbered 1,2,3,4 respectively, control group and test group are set, control group 1 does not do any process, throws in the complex microorganism preparations obtained by above-described embodiment 1-3 respectively to 2,3, No. 4 culturing pools.Nitrite, ammonia nitrogen, hydrogen sulfide content and COD value is measured respectively before input compound formulation.
The complex microorganism preparations that the above embodiment of the present invention prepares by 30 grams/acre of rice consumptions by test group is directly shed near oxygenating machine, starts oxygenating machine I hour, and microbial preparation is fully scattered, and action effect is more abundant.Every other day the same period gets control group and test group water sample carries out water quality detection respectively, and detection comprises: nitrite, ammonia nitrogen, hydrogen sulfide content and COD value, continuous detecting 7 days.Result is as following table
Shown in:
Nitrite content measures (mg/L)
First day Second day 3rd day 4th day 5th day 6th day 7th day
No. 1 pond 0.0738 0.0743 0.0756 0.0767 0.0771 0.0779 0.0783
No. 2 ponds 0.0742 0.0652 0.0546 0.0432 0.0328 0.0254 0.0171
No. 3 ponds 0.0739 0.0659 0.0562 0.0435 0.0335 0.0273 0.0202
No. 4 ponds 0.0736 0.0648 0.0523 0.0434 0.0327 0.0268 0.0192
Ammonia-nitrogen content measures (mg/L)
First day Second day 3rd day 4th day 5th day 6th day 7th day
No. 1 pond 0.162 0.167 0.168 0.171 0.173 0.177 0.181
No. 2 ponds 0.158 0.154 0.148 0.136 0.124 0.108 0.087
No. 3 ponds 0.164 0.157 0.148 0.135 0.121 0.109 0.091
No. 4 ponds 0.167 0.162 0.153 0.142 0.128 0.116 0.095
Hydrogen sulfide content measures (mg/L)
First day Second day 3rd day 4th day 5th day 6th day 7th day
No. 1 pond 0.0398 0.0415 0.0396 0.0427 0.0413 0.0432 0.0438
No. 2 ponds 0.0422 0.0402 0.0388 0.0296 0.0164 0.0138 0.0109
No. 3 ponds 0.0389 0.0357 0.0268 0.0175 0.0121 0.0112 0.0098
No. 4 ponds 0.0412 0.0361 0.0254 0.0141 0.0127 0.0116 0.0102
COD value measures
First day Second day 3rd day 4th day 5th day 6th day 7th day
No. 1 pond 9.605 9.615 9.616 9.621 9.623 9.628 9.637
No. 2 ponds 9.508 9.302 9.213 9.016 8.872 8.433 8.109
No. 3 ponds 9.627 9.327 9.218 9.019 8.885 8.452 8.118
No. 4 ponds 9.504 9.321 9.204 9.011 8.827 8.381 8.102
Drop into the control group that any process is not done in the later contrast of complex microorganism preparations as can be seen from the above table, the content of the objectionable impuritiess such as aquaculture water nitrite, ammonia nitrogen, hydrogen sulfide is all decreased significantly, and the COD value of water body also has obvious reduction.The loss that the water pollution using this complex microorganism preparations can greatly reduce sea farming is brought to cultivation factory, simultaneously significant to the ecological environmental protection of ocean.

Claims (6)

1. a water body purification technology, is characterized in that: utilize complex microorganism preparations purifying sea water aquaculture water.
2. the component that according to claim 1, complex microorganism preparations comprises has: subtilis, Bacillus licheniformis, wax genus bacillus, Pseudomonas aeruginosa, red city rhodococcus, addicted to salt pseudomonas, Rhodopseudomonas palustris and faecium.
3. according to claim 2, complex microorganism preparations each component strain deposit number is respectively: subtilis CGMCCNo.2288, Bacillus licheniformis CGMCC1.10257, wax genus bacillus CGMCC1.10163, Pseudomonas aeruginosa CGMCC0190, red city rhodococcus CGMCC1.3613, addicted to salt pseudomonas CCTCC M 2013070, Rhodopseudomonas palustris CGMCC1.2352 and faecium CGMCC1.2334.
4. according to Claims 2 or 3, it is characterized in that: in complex microorganism preparations, each component adopts high density liquid to ferment respectively, makes spore content up to 10 by zymotechnique post-processing technology 10-10 12the hyperconcentration bacterium powder of CFU/g.
5. according to claim 2 or 4, the configuration proportion of each component of complex microorganism preparations is: subtilis 20%-50%, Bacillus licheniformis 5%-15%, bacillus cereus 10%-30%, Pseudomonas aeruginosa 1%-5%, red city rhodococcus 1%-5%, bites salt pseudomonas 5%-10%, Rhodopseudomonas palustris 5%-15%, faecium 15%-40%.
6. according to claim 1, utilize the method for complex microorganism preparations purifying sea water aquaculture water: complex microorganism preparations the present invention prepared by 20-40 gram/acre of rice consumption is directly shed near oxygenating machine, start oxygenating machine 1 hour, microbial preparation is fully scattered.
CN201410556088.5A 2014-10-20 2014-10-20 Mariculture water body purification composite microbial preparation Pending CN104512968A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104894007A (en) * 2015-05-12 2015-09-09 安徽农业大学 Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses
CN105132345A (en) * 2015-10-27 2015-12-09 淮海工学院 Enterococcus sp. Y12 and application thereof
WO2019010116A1 (en) 2017-07-03 2019-01-10 Oakbio Inc. Novel microbial biomass based feed products
CN109554307A (en) * 2015-12-01 2019-04-02 北京德瑞丰农业科技有限责任公司 The purposes of Huo Shi enterobacteria K20 degradation agricultural wastes
CN112322539A (en) * 2020-11-14 2021-02-05 江苏海洋大学 Enterococcus faecium R-NTR-1 from sea and screening method and application thereof
CN114657081A (en) * 2020-12-22 2022-06-24 湖北绿天地生物科技有限公司 Ternary composite bacterium preparation and application thereof
CN115232763A (en) * 2022-06-07 2022-10-25 四川汇邦环保科技有限公司 Compound microbial agent for separating and conditioning high-density culture water body of grass carp

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CN103468621A (en) * 2013-09-29 2013-12-25 青岛蔚蓝生物集团有限公司 Compound microorganism preparation capable of purifying water

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104894007A (en) * 2015-05-12 2015-09-09 安徽农业大学 Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses
CN104894007B (en) * 2015-05-12 2019-07-05 安徽全民环保科技有限公司 Complex microorganism preparations and preparation method and the application in processing animal carcass
CN105132345A (en) * 2015-10-27 2015-12-09 淮海工学院 Enterococcus sp. Y12 and application thereof
CN109554307B (en) * 2015-12-01 2021-10-26 海林市中农国泰生物科技有限公司 Application of enterobacter hopcalis K20 in degradation of agricultural wastes
CN109554307A (en) * 2015-12-01 2019-04-02 北京德瑞丰农业科技有限责任公司 The purposes of Huo Shi enterobacteria K20 degradation agricultural wastes
EP3649233A4 (en) * 2017-07-03 2020-12-16 Oakbio Inc. Novel microbial biomass based feed products
JP2020526230A (en) * 2017-07-03 2020-08-31 オークバイオ インコーポレイテッドＯａｋｂｉｏ Ｉｎｃ． Feed products based on new microbial biomass
WO2019010116A1 (en) 2017-07-03 2019-01-10 Oakbio Inc. Novel microbial biomass based feed products
US11439172B2 (en) 2017-07-03 2022-09-13 Oakbio, Inc. Microbial biomass based feed products
JP7240751B2 (en) 2017-07-03 2023-03-16 オークバイオ インコーポレイテッド Feed products based on novel microbial biomass
CN112322539A (en) * 2020-11-14 2021-02-05 江苏海洋大学 Enterococcus faecium R-NTR-1 from sea and screening method and application thereof
CN114657081A (en) * 2020-12-22 2022-06-24 湖北绿天地生物科技有限公司 Ternary composite bacterium preparation and application thereof
CN115232763A (en) * 2022-06-07 2022-10-25 四川汇邦环保科技有限公司 Compound microbial agent for separating and conditioning high-density culture water body of grass carp

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Application publication date: 20150415