CN115232763A - Compound microbial agent for separating and conditioning high-density culture water body of grass carp - Google Patents
Compound microbial agent for separating and conditioning high-density culture water body of grass carp Download PDFInfo
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- CN115232763A CN115232763A CN202210640344.3A CN202210640344A CN115232763A CN 115232763 A CN115232763 A CN 115232763A CN 202210640344 A CN202210640344 A CN 202210640344A CN 115232763 A CN115232763 A CN 115232763A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
The invention relates to the technical field of microbial agents, and discloses a compound microbial agent for conditioning high-density culture water of grass carp, which comprises the following components in percentage by mass: 13% of bacillus subtilis subspecies, 17% of bacillus licheniformis, 16% of rhodopseudomonas palustris, 13% of debaryomyces hansenii, 23% of lactobacillus plantarum and 18% of moist cellulomonas. The microbial inoculum can be applied to the water conditioning modification of the early stage of the high-density grass carp culture water body, and can decompose and utilize harmful substances such as ammonia nitrogen, nitrite and the like in the water in the culture process, inhibit the growth of harmful algae, eliminate peculiar smell, reduce the using amount of antibiotics and have good ecological benefit and environmental protection benefit.
Description
Technical Field
The invention relates to the technical field of microbial agents, and particularly relates to a compound microbial agent for separating and conditioning high-density culture water of grass carps.
Background
Grass carp (Ctenophagodonidellus) is commonly called grass carp and white grass carp, belongs to Cyprinales, cyprinaceae, ocimum, grass carp, and is a unique fish species in plain areas from Guangxi to Heilongjiang in China. Has now migrated to many countries in asia, europe, america, africa. Grass carp as one of four kinds of Chinese freshwater aquaculture is deeply favored by farmers due to fast growth speed and wide feed source, is cultured from south to north, becomes one of leading varieties of Chinese aquaculture, and has the first position of the yield of edible aquaculture varieties throughout the year.
For a long time, the main modes of artificial culture of grass carps include high-density purse net culture, net cage culture, circulating water culture, culture pond culture and the like. With the continuous enlargement of the culture area, the disease problem also comes with: the grass carp cultured in the culture pond has large bearing capacity and insufficient self-purification capacity of water, so that the immunity of the grass carp is reduced and the disease resistance is poor; and the fish excretes in the pond, and the excrement deposits to the bottom of the pond, and the breed pond is difficult to thoroughly clear up the fish excrement, adopts thoroughly to trade water clearance usually, and work load is big, also influences the stability of the normal growth environment of fish, influences breed output and benefit when serious.
Antibiotics and halogen chemical sterilizing agents are adopted for prevention and treatment at present; however, the early-stage use effect of the antibiotics is good, the drug effect gradually disappears along with the use times, the main reason is the generation of biological drug resistance, most halogen chemical sterilizing agents contain strong oxidants such as chlorine, iodine, bromine and the like, the corrosivity is strong, the safety factor is low, and the survival of fishes can be threatened once the dosage of the drugs is not properly controlled. For example, the chemical water quality modifier mainly comprises an oxidant, an ion exchanger, a flocculating agent and the like, has a bacteriostatic action, can react with harmful substances to degrade the harmful substances, but has a larger influence on the using effect by water quality conditions, has irritation to aquatic animals, and particularly has a larger adverse influence on the animals in the seedling stage. The biological water quality modifier is the most used type at present, can inhibit the growth and reproduction of pathogenic bacteria, thoroughly decompose harmful substances in water, has no adverse effect on aquatic animals, and restores the ecological environment of the water.
The use of the existing microbial preparation for improving the water quality of grass carp culture is greatly influenced by external conditions such as temperature, medicine residue and the like, the use requirement is high, and the actual effect fluctuates greatly.
Disclosure of Invention
Based on the problems, the invention provides the compound microbial agent for separating and conditioning the high-density grass carp culture water, which can be applied to early water conditioning modification of the high-density grass carp culture water, and can decompose and utilize harmful substances such as ammonia nitrogen, nitrite and the like in water in the culture process, inhibit the growth of harmful algae, eliminate peculiar smell, reduce the using amount of antibiotics and has good ecological benefit and environmental protection benefit.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
the compound microbial agent for separating and conditioning the high-density culture water body of the grass carp mainly comprises the following microbial agents in percentage by mass: 13% of bacillus subtilis subspecies, 17% of bacillus licheniformis, 16% of rhodopseudomonas palustris, 13% of debaryomyces hansenii, 23% of lactobacillus plantarum and 18% of moist cellulomonas.
Further, the number of viable cells of each component strain is ≧ 5 × 10 9 CFU/gram.
In order to realize the technical effects, the invention also provides an application of the compound microbial agent for separating and conditioning the high-density grass carp culture water body, and the compound microbial agent is used for modifying and regulating the culture water body before the grass carps are put in the culture water body, or is added in the high-density grass carp culture process for use, and is used for decomposing harmful substances in the water body.
Further, the compound microbial agent is added or sprayed into the grass carp culture water body, and the dosage and the operation method are as follows: at normal temperature, the microbial inoculum is uniformly dissolved in water and sprayed or flushed on water, and the using amount of the microbial inoculum is 7-15g/m 3 。
Compared with the prior art, the invention has the beneficial effects that: the composite microbial agent for decomposing and conditioning the high-density grass carp culture water body is prepared by mixing 13% of bacillus subtilis subspecies, 17% of bacillus licheniformis, 16% of rhodopseudomonas palustris, 13% of debaryomyces hansenii, 23% of lactobacillus plantarum and 18% of moist fiber monad, and can be applied to the early-stage water conditioning modification of the high-density grass carp culture water body and the decomposition and utilization of harmful substances such as ammonia nitrogen and nitrite in water in the culture process, so that the growth of harmful algae is inhibited, and the peculiar smell is eliminated; can also reduce the dosage of antibiotics, and has good ecological benefit and environmental protection benefit.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
The embodiment is as follows:
the compound microbial agent for separating and conditioning the high-density aquaculture water of the grass carps mainly comprises the following microbial agents in percentage by mass: 13% of bacillus subtilis subspecies, 17% of bacillus licheniformis, 16% of rhodopseudomonas palustris, 13% of debaryomyces hansenii, 23% of lactobacillus plantarum and 18% of moist cellulomonas.
The compound microbial agent is used for modifying and adjusting the culture water body before the grass carp is put in the culture, or is added in the high-density culture process of the grass carp and is used for decomposing harmful substances in the water body.
The compound microbial agent in the embodiment can be applied to the early-stage water regulation modification of the high-density grass carp culture water body, and can decompose and utilize harmful substances such as ammonia nitrogen, nitrite and the like in water in the culture process, inhibit the growth of harmful algae and eliminate peculiar smell. Particularly, when the compound microbial agent is added or sprayed into a grass carp culture water body, the dosage range and the operation method are as follows: at normal temperature, the microbial inoculum is uniformly dissolved in water and sprayed or flushed on water, and the using amount of the microbial inoculum is 7-15g/m 3 (ii) a Can be harmful to the grass carp culture water bodyThe material is decomposed efficiently; inhibiting growth of harmful algae, and eliminating odor. The compound microbial agent can be used for producing a large amount of specific active biological enzymes through synergistic effects such as metabolic complementation and intercropping among microbial communities, synergistically and intensively degrading protein, cellulose, hemicellulose and the like in grass carp feed residues and excrement, and fully absorbing and converting harmful substances such as ammonia nitrogen, nitrite and the like in water, so that a high-efficiency water purification function is achieved, the using amount of antibiotics can be reduced, and the compound microbial agent has good ecological benefit and environmental protection benefit.
In the embodiment, the number of viable cells of each component strain of the compound microbial agent is not less than 5 × 10 9 CFU/g. The living cell density is high, the synergistic effect among microbial populations is strong, the high-efficiency decomposition of harmful substances in the grass carp culture water body can be ensured, and the environment of the grass carp culture water body is further ensured.
The above is the embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the process of verifying the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all the equivalent structural changes made by applying the content of the specification of the invention should be covered by the scope of the invention.
Claims (4)
1. The compound microbial agent for separating and conditioning the high-density culture water body of the grass carp is characterized by mainly comprising the following microbial agents in percentage by mass: 13% of bacillus subtilis subspecies, 17% of bacillus licheniformis, 16% of rhodopseudomonas palustris, 13% of debaryomyces hansenii, 23% of lactobacillus plantarum and 18% of moist cellulomonas.
2. The compound microbial agent for decomposing and conditioning high-density aquaculture water for grass carp as claimed in claim 1, wherein the number of living cells of each component strain is ≧ 5 × 10 9 CFU/gram.
3. The application of the compound microbial agent for decomposing and conditioning the high-density grass carp culture water body according to claim 1 or 2, wherein the compound microbial agent is used for modifying and conditioning the culture water body before the grass carps are put into the culture water body, or is used for decomposing harmful substances in the water body after being added into the culture water body in the high-density grass carp culture process.
4. The application of the compound microbial agent for decomposing and conditioning the high-density aquaculture water body of the grass carps as claimed in claim 3, wherein the compound microbial agent is added or sprayed into the aquaculture water body of the grass carps, and the dosage and the operation method are as follows: at normal temperature, the microbial inoculum is uniformly dissolved in water and sprayed or flushed on water, and the using amount of the microbial inoculum is 7-15g/m 3 。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210640344.3A CN115232763A (en) | 2022-06-07 | 2022-06-07 | Compound microbial agent for separating and conditioning high-density culture water body of grass carp |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210640344.3A CN115232763A (en) | 2022-06-07 | 2022-06-07 | Compound microbial agent for separating and conditioning high-density culture water body of grass carp |
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| Publication Number | Publication Date |
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| CN115232763A true CN115232763A (en) | 2022-10-25 |
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| CN202210640344.3A Pending CN115232763A (en) | 2022-06-07 | 2022-06-07 | Compound microbial agent for separating and conditioning high-density culture water body of grass carp |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030086477A (en) * | 2003-09-24 | 2003-11-10 | 최성호 | Microbial Agent for Waste Water Treatment |
| CN104388348A (en) * | 2014-11-21 | 2015-03-04 | 上海寄绿生物环保科技有限公司 | Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof |
| CN104512968A (en) * | 2014-10-20 | 2015-04-15 | 镇江拜因诺生物科技有限公司 | Mariculture water body purification composite microbial preparation |
| CN107043718A (en) * | 2017-03-01 | 2017-08-15 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and its preparation method and application |
| CN107557318A (en) * | 2017-09-25 | 2018-01-09 | 黄灵君 | For removing the microorganism formulation of hydrogen sulfide malodorant |
| WO2018133411A1 (en) * | 2017-01-17 | 2018-07-26 | 广州市广深环保科技有限公司 | Multifunctional and efficient sewage microorganism activated bacterial agent and use thereof |
| CN111793575A (en) * | 2020-06-03 | 2020-10-20 | 惠州学院 | Complex microbial inoculant and application thereof in aquaculture |
-
2022
- 2022-06-07 CN CN202210640344.3A patent/CN115232763A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030086477A (en) * | 2003-09-24 | 2003-11-10 | 최성호 | Microbial Agent for Waste Water Treatment |
| CN104512968A (en) * | 2014-10-20 | 2015-04-15 | 镇江拜因诺生物科技有限公司 | Mariculture water body purification composite microbial preparation |
| CN104388348A (en) * | 2014-11-21 | 2015-03-04 | 上海寄绿生物环保科技有限公司 | Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof |
| WO2018133411A1 (en) * | 2017-01-17 | 2018-07-26 | 广州市广深环保科技有限公司 | Multifunctional and efficient sewage microorganism activated bacterial agent and use thereof |
| CN107043718A (en) * | 2017-03-01 | 2017-08-15 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and its preparation method and application |
| CN107557318A (en) * | 2017-09-25 | 2018-01-09 | 黄灵君 | For removing the microorganism formulation of hydrogen sulfide malodorant |
| CN111793575A (en) * | 2020-06-03 | 2020-10-20 | 惠州学院 | Complex microbial inoculant and application thereof in aquaculture |
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