CN105886440A - Bacillus licheniformis and microbial agent and application and method of microbial agent in treatment of agricultural wastes - Google Patents
Bacillus licheniformis and microbial agent and application and method of microbial agent in treatment of agricultural wastes Download PDFInfo
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Abstract
The invention discloses bacillus licheniformis and a microbial agent and an application and method of the microbial agent in treatment of agricultural wastes. The preservation number of the bacillus licheniformis is CGMCC No.12316. The invention further provides the microbial agent. The microbial agent can be used for treating the agricultural wastes. By the treatment method of the agricultural wastes, a large quantity of agricultural wastes including straws, livestock breeding wastewater, animal dung and the like can be simultaneously treated; meanwhile, no wastewater is discharged in the treatment process; and the pollution to the environment is reduced. The treatment method of the agricultural wastes has the advantages of high treatment efficiency, no pollution, small floor area, low energy consumption and high economic benefits.
Description
Technical field
The present invention relates to agricultural wastes process field, in particular it relates to a bacillus licheniformis and microbial inoculum and the application in processing agricultural wastes thereof and processing method.
Background technology
Agricultural wastes are that agricultural production, processing of farm products, livestock and poultry breeding industry and urban residents live the general name of garbage of discharge.
Large-scale animal farm and all discharge substantial amounts of feces and corral, the wall to wall of pouity dwelling place with the place of drylot feeding mode extensive raise poultry domestic animal, enters environment if untreated, just pollutes the environment.The most undressed feces enters rivers and lakes, and water quality can be made dirty, and Biochemical oxygen demand (BOD) load increases, and forms anaerobism corruption or eutrophication, threatens Fish, shellfish and the existence of algae；Also can infect disease, affect residents ' health.If irrigation water is by the severe contamination of agricultural wastes, the ammonia nitrogen in water and protein nitrogen content can be made too high, thus cause Oryza sativa L. excessive growth, lodging, late-maturing or not yet done；Furthermore, it is also possible to make the nitrate having excess in underground aquifers, or surrounding is made to multiply a large amount of fly and other insects.
Along with the scale of China's livestock culture industry constantly expands, livestock culture Pollution exposure environmental problem out is the most increasing, substantial amounts of poultry Excreta, the discharge of various breeding waste, not only have impact on the overall efficiency of livestock culture, and polluted source, cause body eutrophication, natural environment and health are all existed harm greatly.Livestock breeding wastewater mainly includes urine, part excrement and breeding house flushing water, and such concentration of organic wastewater is high, float is many, ammonia-nitrogen content is high, stink is big.The materials such as Organic substance in these waste water, ammonia nitrogen, without process, are directly discharged into water body, water body about will cause serious eutrophication, the self-cleaning ability of heavy damage water body, cause water body blackout to foul, affect environment and agricultural irrigation.
The method processing agricultural wastes in prior art is the most single, for agriculturalStraw stalkMainly take Biogas and preparation Agricultural organic fertilizer, or as the roughage of raise livestock and hurdle circle place mat material；And the processing method of breeding wastewater mainly includes physical treatment process, method of chemical treatment and biological treatment three class.The biochemical treatment method being widely used at present is to utilize microbial metabolism effect, makes in stain disease in dissolving, the organic pollution of colloidal state is converted into a kind of processing method of stable innocuous substance.Can be divided mainly into two big classes, i.e. make good use of the aerobic method (aerobic oxidation method) of oxygen animalcule effect and utilize the anaerobic process (anaerobic reduction method) of anaerobe effect.The former is widely used in Treating Municipal Sewage and Organic production waste, the most active sludge and biomembrance process two kinds；The latter is used for processing high concentration organic sewage and the mud of generation in sewage disposal process, also begins to now for Treating Municipal Sewage and low-concentration organic wastewater.Also lack the comprehensive processing method that various agricultural garbage is processed simultaneously at present, the defects such as the most existing processing method there is also that energy consumption is big, take up an area wide, investment and operating cost is high, and the mud produced in processing procedure and waste water still need to discharge outside system, cause bigger ambient pressure.
Summary of the invention
It is an object of the invention to provide a bacillus licheniformis and microbial inoculum and the application in processing agricultural wastes thereof and processing method, this processing method can solve to lack the comprehensive processing method simultaneously processed various agricultural garbage in prior art, the mud and the waste water that produce in the defects, and processing procedure such as the most existing processing method there is also that energy consumption is big, take up an area wide, investment and operating cost is high still need to the technical problem of discharge outside system.
The present inventor has separated a bacillus licheniformis, find that it can be efficiently by nitrogen source that nitrogen transformation is Lumbricus growth needs, ammonium nitrogen is converted into nitrite nitrogen thus reduces the generation of ammonia nitrogen, the most also have and cellulose degradation is become humic acid material, resulting in the present invention.
To achieve these goals, the present invention provides a bacillus licheniformis, this Bacillus licheniformisPreservationNumbered CGMCC No.12316.
The present invention also provides for a kind of microbial bacterial agent, and this microbial bacterial agent contains thalline and culture medium, and thalline containsPreservationThe Bacillus licheniformis of numbered CGMCC No.12316.
The present invention also provides for the application in processing agricultural wastes of the mentioned microorganism microbial inoculum, and described agricultural wastes are one or more in straw, fowl and animal excrement and livestock culture waste water.
The present invention also provides for the method that mentioned microorganism microbial inoculum processes agricultural wastes, and the method comprises the following steps:
(1) make described microbial bacterial agent mix homogeneously with described agricultural wastes and obtain earthworm cultivation substratum, regulate the moisture content of described earthworm cultivation substratum to 50-80 weight %；
Wherein, described agricultural wastes contain straw and livestock culture waste water, and relative to 100 weight portion straws, the consumption of described livestock culture waste water is 500-1000 weight portion, the consumption of described microbial bacterial agent is 0.5-5 weight portion, and the total nitrogen concentration of described livestock culture waste water is 100-200mg/L；
(2) inoculating Lumbricus in described earthworm cultivation substratum, wherein, relative to 100 weight portion straws, the inoculum density of described Lumbricus is 20-40 bar；
(3) making described Lumbricus cultivate 50-90 days in earthworm cultivation substratum, in breeding process, add livestock culture waste water in earthworm cultivation substratum, addition is 10-30 weight portion/sky；
(4) after breeding process terminates, being separated with wormcast by Lumbricus, Lumbricus is used for livestock culture feedstuff and pharmacy industry raw material, and wormcast plants fertilizer for fruit and vegerable.
Pass through technique scheme, the Bacillus licheniformis of the present invention and microbial bacterial agent can be with decomposition of cellulose, generation humic acid, degradation of ammonia nitrogen, this microbial bacterial agent and agricultural wastes it is mixed earthworm cultivation substratum and uses this cultivation stromal feeders Lumbricus, in breeding process, continuing to add livestock culture waste water simultaneously can promote that cocoon is produced in Lumbricus weightening finish.The agriculture waste substance treating method of the present invention can consume a large amount of agricultural wastes during breeding earthworm, and whole during agricultural wastes digested by vermiculture process completely, without arranging outside waste water；Lumbricus and wormcast that cultivation obtains can be used separately as the feedstuff of livestock culture and the fertilizer of plant husbandry, have considerable economic benefit.The agriculture waste substance treating method of the present invention has that treatment effeciency is high, pollution-free, take up an area less, energy consumption is low and the advantage of high financial profit.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
BiomaterialPreservation
The Bacillus licheniformis (Bacillus licheniformis) of the present invention is the present inventor from being positioned at Pekinese's cattle manure and heap corruption straw the pure culture separated, itsPreservationNumbered CGMCC No.12316,PreservationDate is on March 29th, 2016,PreservationUnit is Chinese microorganism strainPreservationManagementCommitteeCommon micro-organisms center, address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, and Classification And Nomenclature is Bacillus licheniformis.
Detailed description of the invention
Hereinafter the detailed description of the invention of the present invention is described in detail.It should be appreciated that detailed description of the invention described herein is merely to illustrate and explains the present invention, it is not limited to the present invention.
The present invention provides a bacillus licheniformis, this Bacillus licheniformisPreservationNumbered CGMCC No.12316.
The present invention also provides for a kind of microbial bacterial agent, and this microbial bacterial agent contains thalline and culture medium, and thalline containsPreservationThe Bacillus licheniformis of numbered CGMCC No.12316.
According to the present invention, the amount of the thalline contained in microbial bacterial agent can in very large range change, such as in every gram microbial bacterial agent,PreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316 can be 107-1011Cfu, under preferable case, in every gram of microbial bacterial agent,PreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316 can be 108-109cfu。
According to the present invention, the thalline of microbial bacterial agent can also be the Rhizopus oryzae of ACCC32592 containing the bacillus thuringiensis that article number is ACCC01954 and article number.Wherein, the cfu content of bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 can in very large range change, under preferable case,PreservationThe cfu content ratio of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 can be 1:(0.5-1): (0.3-0.6).PreservationWhen the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 are in above-mentioned content range, the efficiency of microbial bacterial agent degradation of ammonia nitrogen is high, the humic acid produced is more, be conducive to improving the weightening finish of the nutrient structure of earthworm cultivation substratum, beneficially Lumbricus and produce cocoon.
According to the present invention, the kind of culture medium can in very large range change, can be that various can be used in cultivates Bacillus licheniformis, bacillus thuringiensis and the culture medium of Rhizopus oryzae, can be commercially available for example, it is possible to make above-mentioned culture medium for conventional culture medium such as beef-protein medium, nutrient broth medium, LB culture medium or prepare according to the record of " microbiological culture media handbook " (Microbiology Culture Media Manual).Such as, culture medium can be beef-protein medium, and it contains the Carnis Bovis seu Bubali cream of 1-5g/L, the peptone of 5-20g/L and the sodium chloride of 3-8g/L.
Wherein, above-mentioned various culture medium are standby after can carrying out sterilizing according to conventional sterilizing methods, such as sterilizing 10-30 minute under conditions of 115-125 DEG C and 1.5-2 normal atmosphere.
Wherein, the preparation method of described microbial bacterial agent may include that byPreservationThe Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 are inoculated in culture medium respectively and cultivate.Under preferable case, by cultivate to every gram of described microbial bacterial agent in,PreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is respectively 107-1011Cfu, more preferably 108-109cfu.During cultivating, can be by conventional method, such as blood cell plate counting method or OD value observational method obtain the concentration of viable bacteria.
Bacterium solution after cultivation can use directly as microbial bacterial agent, and under preferable case, bacterium solution is by including that the step such as aseptic filtration, lyophilization is further processed into the microbial bacterial agent use of the dosage form of more convenient storage.Wherein, the condition of culture of strain has no particular limits, and can be typical conditions during spawn culture, cultivates for example with shaking table concussion, and cultivation temperature can be 28-37 DEG C, and incubation time can be 1-3 days.
Present invention also offers the application in processing agricultural wastes of Bacillus licheniformis as above and microbial bacterial agent as above.
Present invention also offers Bacillus licheniformis as above and the method for microbial bacterial agent as above process agricultural wastes, the method comprises the following steps:
(1) make described microbial bacterial agent mix homogeneously with described agricultural wastes and obtain earthworm cultivation substratum, regulate the moisture content of described earthworm cultivation substratum to 50-80 weight %；
Wherein, described agricultural wastes contain straw and livestock culture waste water, and relative to 100 weight portion straws, the consumption of described livestock culture waste water is 500-1000 weight portion, the consumption of described microbial bacterial agent is 0.5-5 weight portion, and the total nitrogen concentration of described livestock culture waste water is 100-200mg/L；
(2) inoculating Lumbricus in described earthworm cultivation substratum, wherein, relative to 100 weight portion straws, the inoculum density of described Lumbricus is 20-40 bar；
(3) making described Lumbricus cultivate 50-90 days in earthworm cultivation substratum, in breeding process, add livestock culture waste water in earthworm cultivation substratum, addition is 10-30 weight portion/sky；
(4) after breeding process terminates, being separated with wormcast by Lumbricus, Lumbricus is used for livestock culture feedstuff and pharmacy industry raw material, and wormcast plants fertilizer for fruit and vegerable.
The Bacillus licheniformis of the present invention and microbial bacterial agent can be with decomposition of cellulose, generation humic acid, degradation of ammonia nitrogen, this microbial bacterial agent and agricultural wastes it is mixed earthworm cultivation substratum and uses this cultivation stromal feeders Lumbricus, in breeding process, continuing to add livestock culture waste water simultaneously can promote that cocoon is produced in Lumbricus weightening finish.The agriculture waste substance treating method of the present invention can consume a large amount of agricultural wastes during breeding earthworm, and whole during agricultural wastes digested by vermiculture process completely, without arranging outside waste water；Lumbricus and wormcast that cultivation obtains can be used separately as the feedstuff of livestock culture and the fertilizer of plant husbandry, have considerable economic benefit.The agriculture waste substance treating method of the present invention has that treatment effeciency is high, pollution-free, take up an area less, energy consumption is low and the advantage of high financial profit
In treatment in accordance with the present invention method, above-mentioned agricultural wastes can also contain fowl and animal excrement, and the consumption of fowl and animal excrement can in very large range change, and under preferable case, relative to 100 weight portion straws, the consumption of fowl and animal excrement can be 10-50 weight portion.Consumption fowl and animal excrement within the above range can make the earthworm cultivation substratum prepared be more suitable for growth and the breeding of Lumbricus, is conducive to consuming more agricultural wastes.
In treatment in accordance with the present invention method, the moisture content of fowl and animal excrement can in very large range change, and under preferable case, the moisture content of fowl and animal excrement can be 6-20%.Moisture content fowl and animal excrement within the above range is conducive to mixing homogeneously with straw, microbial bacterial agent and livestock culture waste water, and makes earthworm cultivation substratum keep suitable moisture content to improve the rate of body weight gain of Lumbricus and to produce cocoon rate.
In treatment in accordance with the present invention method, the not particularly requirement of the kind of straw, the straw kind that can be well known to those skilled in the art, such as, can be the agricultural crop straws such as Oryza sativa L., Semen Maydis, Semen Tritici aestivi.The particle diameter of straw and fowl and animal excrement can in very large range change, and under preferable case, the particle diameter of straw and fowl and animal excrement can be 5-10mm.Straw and fowl and animal excrement in above-mentioned particle size range are conducive to mixing homogeneously with microbial bacterial agent and livestock culture waste water.
In treatment in accordance with the present invention method, livestock culture waste water can be from pig, cattle, sheep, duck, chicken and the waste water of E Deng plant, its C/N can in very large range change than with total nitrogen concentration, and the total nitrogen concentration of livestock culture waste water is preferably 100-200mg/L.
In treatment in accordance with the present invention method, the pH of earthworm cultivation substratum not particularly requirement, can be well known to those skilled in the art, the pH of such as earthworm cultivation substratum can be 7-9.The earthworm cultivation substratum meeting above-mentioned requirements is conducive to Lumbricus weightening finish and produces cocoon, and can consume the livestock culture waste water of more amount.
In treatment in accordance with the present invention method, the not particularly requirement of the Lumbricus kind of cultivation, the kind that can be well known to those skilled in the art, such as Lumbricus can be one or more in Eisenia foetida (Eisenia foetida), Pheretimatschiliensis (Pheretima tschiliensis), different lip earthworm (Allolobophora) and Lumbricidae Bimostos (Bimastus), can preferably be Eisenia foetida.The Lumbricus of mentioned kind can consume more livestock culture waste water in growth course, possesses higher rate of body weight gain when the earthworm cultivation substratum of the present invention grows and produces cocoon rate.
Further illustrate the present invention below by embodiment, but the present invention is not therefore subject to any restriction.
Embodiment 1
The present embodiment is for illustrating cultivation and the preparation of microbial bacterial agent of the Bacillus licheniformis of the present invention.
WillPreservationThe Bacillus licheniformis of numbered CGMCC No.12316 is inoculated in LB culture medium (NaCl of tryptone, the yeast extract of 5g/L and 10g/L containing 10g/L), cultivates under 30 DEG C and 200 revs/min vibrations.The bacterium solution of gained is the microbial bacterial agent V1 of the present embodiment, and in every gram of this microbial bacterial agent, viable count is 109cfu。
Embodiment 2
The present embodiment is for illustrating cultivation and the preparation of microbial bacterial agent of the Bacillus licheniformis of the present invention.
(1) willPreservationThe Bacillus licheniformis of numbered CGMCC No.12316, article number are the bacillus thuringiensis of ACCC01954 and the Rhizopus oryzae that article number is ACCC32592 is inoculated in beef-protein medium (NaCl of peptone, the Carnis Bovis seu Bubali cream of 5g/L and 5g/L containing 20g/L) respectively, cultivate under 37 DEG C and 180 revs/min vibrations, incubation is sampled and is observed by ascites method, untilPreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 respectively reaches 108Cfu/ gram.
(2) carry out in proportion mixing by the Bacillus licheniformis obtained in (1), bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 so as to get microbial bacterial agent V2 in,PreservationThe cfu content of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is than for 1:0.5:0.6.
Embodiment 3
The present embodiment is for illustrating cultivation and the preparation of microbial bacterial agent of the Bacillus licheniformis of the present invention.
(1) willPreservationThe Bacillus licheniformis of numbered CGMCC No.12316, article number are the bacillus thuringiensis of ACCC01954 and the Rhizopus oryzae that article number is ACCC32592 is inoculated in nutrient broth medium (peptone, the Carnis Bovis seu Bubali cream powder of 3g/L and the NaCl of 5g/L containing 10g/L) respectively, cultivate under 35 DEG C and 160 revs/min vibrations, incubation is sampled and is observed by ascites method, untilPreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 respectively reaches 2 × 108Cfu/ gram.
(2) carry out in proportion mixing by the Bacillus licheniformis obtained in (1), bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 so as to get microbial bacterial agent V3 in,PreservationThe cfu content of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is than for 1:1:0.3.
Embodiment 4
The present embodiment is for illustrating cultivation and the preparation of microbial bacterial agent of the Bacillus licheniformis of the present invention.
(1) willPreservationThe Bacillus licheniformis of numbered CGMCC No.12316, article number are the bacillus thuringiensis of ACCC01954 and the Rhizopus oryzae that article number is ACCC32592 is inoculated in nutrient broth medium (peptone, the Carnis Bovis seu Bubali cream powder of 3g/L and the NaCl of 5g/L containing 10g/L) respectively, cultivate under 35 DEG C and 160 revs/min vibrations, incubation is sampled and is observed by ascites method, untilPreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 respectively reaches 8 × 108Cfu/ gram.
(2) carry out in proportion mixing by the Bacillus licheniformis obtained in (1), bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 so as to get microbial bacterial agent V4 in,PreservationThe cfu content of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is than for 1:0.6:0.5.
Embodiment 5
The present embodiment is for illustrating cultivation and the preparation of microbial bacterial agent of the Bacillus licheniformis of the present invention.
Use cultural method the same as in Example 4, in the microbial bacterial agent V5 obtained,PreservationThe cfu content of the Bacillus licheniformis of numbered CGMCC No.12316 is 107Cfu/ gram.
Embodiment 6
The present embodiment is for illustrating cultivation and the preparation of microbial bacterial agent of the Bacillus licheniformis of the present invention.
Cultural method the same as in Example 4 is used to obtain microbial bacterial agent V6, except that, the cfu content such as bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is replaced with respectivelyPreservationThe Bacillus licheniformis of numbered CGMCC No.12316.
Embodiment 7
Use cultural method the same as in Example 4, except that, in the microbial bacterial agent V7 obtained,PreservationThe cfu content of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is than for 1:2:2.
Comparative example 1
This comparative example is for illustrating the microbial bacterial agent different from the present invention.
Cultural method the same as in Example 4 is used to obtain microbial bacterial agent V8, except that, willPreservationThe Bacillus licheniformis of numbered CGMCC No.12316 such as replaces with at the bacillus thuringiensis that article number is ACCC01954 of cfu content.
Comparative example 2
This comparative example is for illustrating the microbial bacterial agent different from the present invention.
Use cultural method the same as in Example 4, except that, by microbial bacterial agentPreservationThe Bacillus licheniformis of numbered CGMCC No.12316 such as replaces with at the article number of cfu content14580TMBacillus licheniformis obtain the microbial bacterial agent V9 of this comparative example.
Comparative example 3
This comparative example is for illustrating the microbial bacterial agent different from the present invention.
Use cultural method the same as in Example 4, except that, microbial bacterial agent is adopted and prepares with the following method: (1) 100 weight portions nutrient culture medium (content of peptone be 0.8 weight %, the content of NaCl be 0.4 weight %, the content of Carnis Bovis seu Bubali cream be 0.45 weight %, surplus be sterilized water, pH is 7.0) in the bacterial strain of Bacillus licheniformis of inoculation 4 weight portions (ACCC 10146, purchased from Chinese agriculture microorganism fungus kindPreservationCenter), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of Bacillus licheniformis is 0.8 × 1010Individual/gram；(2) at the bacterial strain of bacillus subtilis of nutrient inoculation of medium 2 weight portion of 100 weight portions, (ACCC 10629, purchased from Chinese agriculture microorganism fungus kindPreservationCenter), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of bacillus subtilis is 0.8 × 1010Individual/gram culture medium, wherein, in described bouillon media, the content of peptone be 1 weight %, the content of NaCl be 0.25 weight %, the content of Carnis Bovis seu Bubali cream be 0.45 weight %, surplus be sterilized water, pH is 7.0.(3) at the bacterial strain of Bacillus pumilus of nutrient inoculation of medium 3 weight portion of 100 weight portions, (ACCC 10113, purchased from Chinese agriculture microorganism fungus kindPreservationCenter), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of Bacillus pumilus is 0.8 × 1010Individual/gram, wherein, in described bouillon media, the content of peptone be 0.5 weight %, the content of NaCl be 0.5 weight %, the content of Carnis Bovis seu Bubali cream be 0.3 weight %, surplus be sterilized water, pH is 7.0.(4) at the bacterial strain of Bacillus coagulans of YPG inoculation of medium 4 weight portion of 100 weight portions, (ACCC 10229, purchased from Chinese agriculture microorganism fungus kindPreservationCenter), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of Bacillus coagulans is 0.8 × 1010Individual/gram, wherein, in described YPG culture medium, the content of peptone be 0.8 weight %, the content of glucose be 0.3 weight %, the content of yeast extract be 0.8 weight %, surplus be sterilized water, the pH of this culture medium is 7.2.(5) 100 weight portions malt extract medium (Shanghai crystalline substance pure reagent company limited) in inoculation 3 weight portions Wine brewing yeast strain (ACCC 20237, purchased from Chinese agriculture microorganism fungus kindPreservationCenter), 30 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of saccharomyces cerevisiae is 0.8 × 1010Individual/gram culture medium.(6) Gao Shi at 100 weight portions synthesizes the bacterial strain of Streptomyces jingyangensis of inoculation of medium 4 weight portion (ACCC 40126, purchased from Chinese agriculture microorganism fungus kindPreservationCenter), 30 DEG C of fermentations, it is sampled during the fermentation and is observed by ascites method, until the viable count of Streptomyces jingyangensis is 0.8 × 1010Individual/gram, wherein, in described Gause I culture medium, on the basis of the gross weight of this culture medium, the content of soluble starch is 2 weight %, KNO3Content be 0.2 weight %, K2HPO4Content be 0.7 weight %, the content of NaCl be 0.03 weight %, surplus be sterilized water, the pH of this culture medium is 7.2.(7) Bacillus licheniformis that will obtain in step (1) to (6), bacillus subtilis, Bacillus pumilus, Bacillus coagulans, saccharomyces cerevisiae and Streptomyces jingyangensis proportionally mix, make in the microbial bacterial agent V10 obtained, on the basis of total viable count of the microbial bacterial agent obtained, the viable count of Bacillus licheniformis is 15 weight % of total viable count, the viable count of bacillus subtilis is the 15% of total viable count, the viable count of Bacillus pumilus is the 20% of total viable count, the viable count of Bacillus coagulans is the 20% of total viable count, the viable count of saccharomyces cerevisiae is the 15% of total viable count, the viable count of Streptomyces jingyangensis is the 15% of total viable count.
Testing example 1
The method that the present embodiment processes livestock culture waste water for the microbial bacterial agent that the present invention is described.
The subspecies choosing Eisenia foetida (Eisenia fetida) put down greatly No. two, raise big flat No. two Lumbricuss with fresh rice straw and tame, select great-hearted Lumbricus and join in processing system after taming one week.Straw selects rice straw, and being crushed to particle diameter with high speed disintegrator is 10mm.
Taking 100 weight parts water rice straws (particle diameter is 10mm), 0.5 weight portion microbial bacterial agent V1,1000 weight portion cattle farm breeding wastewaters (total nitrogen concentration is 100mg/L) mix homogeneously obtains earthworm cultivation substratum (pH is 7)；Earthworm cultivation substratum is inoculated 20 Lumbricuss；During vermiculture, in earthworm cultivation substratum, add 10 weight portion livestock culture waste water every day；Measure the weightening finish of Lumbricus after cultivating 50 days and produce cocoon rate, and N P and K increase rate, organic reduction rate and the C/N reduction rate in earthworm cultivation substratum, test result is listed inTable 1In.
Testing example 2
The method that the present embodiment processes livestock culture waste water for the microbial bacterial agent that the present invention is described.
The subspecies choosing Eisenia foetida (Eisenia fetida) put down greatly No. two, raise big flat No. two with fresh wheat stalk and tame, select great-hearted Lumbricus and join in processing system after taming one week.Straw selects wheat stalk, and being crushed to particle diameter with high speed disintegrator is 5mm.
Take 100 weight portion wheat stalks (particle diameter is 5mm), 5 weight portion microbial bacterial agent V2 and 500 weight portion pig farm breeding wastewaters (total nitrogen concentration is 200mg/L) mix homogeneously and obtain earthworm cultivation substratum (pH is 7.2)；Earthworm cultivation substratum is inoculated 40 Lumbricuss；During vermiculture, in earthworm cultivation substratum, add 30 weight portion livestock culture waste water every day；Measure weightening finish after cultivating 90 days and produce cocoon rate, and N P and K increase rate, organic reduction rate and the C/N reduction rate in earthworm cultivation substratum, test result is listed inTable 1In.
Testing example 3
The method that the present embodiment processes livestock culture waste water for the microbial bacterial agent that the present invention is described.
The subspecies choosing Eisenia foetida (Eisenia fetida) put down greatly No. two, raise big flat No. two with fresh corn straw and tame, select great-hearted Lumbricus and join in processing system after taming one week.Straw selects corn straw, and being crushed to particle diameter with high speed disintegrator is 10mm.
Take 100 parts by weight Corn straws (particle diameter is 10mm), 4 weight portion microbial bacterial agent V3,10 weight portion fowl and animal excrements and 900 weight portion pig farm breeding wastewaters (total nitrogen concentration is 200mg/L) mix homogeneously obtain earthworm cultivation substratum (pH is 7.3)；Earthworm cultivation substratum is inoculated 25 Lumbricuss；During vermiculture, in earthworm cultivation substratum, add 20 weight portion livestock culture waste water every day；Measure weightening finish after cultivating 80 days and produce cocoon rate, and N P and K increase rate, organic reduction rate and the C/N reduction rate in earthworm cultivation substratum, test result is listed inTable 1In.
Testing example 4
The method that the present embodiment processes livestock culture waste water for the microbial bacterial agent that the present invention is described.
The subspecies choosing Eisenia foetida (Eisenia fetida) put down greatly No. two, raise big flat No. two Lumbricuss with fresh rice straw and tame, select great-hearted Lumbricus and join in processing system after taming one week.Straw selects rice straw, and being crushed to particle diameter with high speed disintegrator is 10mm.
Take 100 weight parts water rice straws (particle diameter is 10mm), 3 weight portion microbial bacterial agent V4,50 weight portion fowl and animal excrements and 850 weight portion pig farm breeding wastewaters (total nitrogen concentration is 200mg/L) mix homogeneously obtain earthworm cultivation substratum (pH is 7.3)；Earthworm cultivation substratum is inoculated 20 Lumbricuss；During vermiculture, in earthworm cultivation substratum, add 25 weight portion livestock culture waste water every day；Measure weightening finish after cultivating 75 days and produce cocoon rate, and N P and K increase rate, organic reduction rate and the C/N reduction rate in earthworm cultivation substratum, test result is listed inTable 1In.
Testing example 5-7
Use the processing method identical with testing example 4, except that, by the microbial bacterial agent V5-V7 of the weight such as microbial bacterial agent V4 replaces to respectively.
Testing example 8
Use the processing method identical with testing example 4, except that, by the livestock culture waste water of the weight such as livestock culture feces replaces with.
Test comparison example 1-3
Use the processing method identical with testing example 4, except that, by the microbial bacterial agent V8-V10 of the weight such as microbial bacterial agent V4 replaces to respectively.
Test comparison example 4
Use the processing method identical with testing example 4, except that, earthworm cultivation substratum is added without microbial bacterial agent.
Test comparison example 5
Use the processing method identical with testing example 4, except that, by the pure water of the weight such as the livestock culture waste water in earthworm cultivation substratum replaces with.
Table
1
According toTable 1Data, testing example 4 can be seen that compared with test comparison example 4-5, relative to using straw and fowl and animal excrement as the microbial bacterial agent (test comparison example 4) being added without the present invention in earthworm cultivation substratum (test comparison example 5) or cultivation substrate, the microbial bacterial agent of the present invention and agricultural wastes are mixed and made into earthworm cultivation substratum can promote that cocoon is produced in Lumbricus weightening finish, and reduces the pollutional load such as total nitrogen, ammonia nitrogen and COD in cultivation waste liquid.Testing example 4 compared with test comparison example 1-3 it can be seen that be added without relative in microbial bacterial agentPreservationThe Bacillus licheniformis of numbered CGMCC No.12316 or be replaced with other bacterium or add other microbial bacterial agent, the microbial bacterial agent of the earthworm cultivation substratum of the present invention can promote Lumbricus weightening finish and produce cocoon, and consumes livestock culture waste water, reduces pollutional load.
From the Data Comparison of testing example 4 and testing example 5 it can be seen that currently preferred every gram of microbial bacterial agent,PreservationThe viable count of the Bacillus licheniformis of numbered CGMCC No.12316 is 108-109In the case of cfu, the microbial bacterial agent of earthworm cultivation substratum can more effectively promote Lumbricus weightening finish and produce cocoon, and consumes livestock culture waste water, reduces pollutional load.
From the Data Comparison of testing example 4 and testing example 6-7 it can be seen that be the Rhizopus oryzae of ACCC32592 possibly together with the bacillus thuringiensis that article number is ACCC01954 and article number the thalline of currently preferred microbial bacterial agent, wherein,PreservationThe cfu content of the Bacillus licheniformis of numbered CGMCC No.12316, bacillus thuringiensis ACCC01954 and Rhizopus oryzae ACCC32592 is than for 1:(0.5-1): in the case of (0.3-0.6), the degradation of ammonia nitrogen of the microbial bacterial agent of the present invention and decomposition of cellulose effective, it is many that Lumbricus fast gaining produces cocoon.
Can be seen that from the Data Comparison of testing example 4 and testing example 8, at currently preferred agricultural wastes possibly together with fowl and animal excrement, relative to 100 weight portion straws, in the case of the consumption of fowl and animal excrement is 10-50 weight portion, the microbial bacterial agent of the present invention processes the method for agricultural wastes to be had the more effective Organic substance decomposed in agricultural wastes and promotes that the effect of cocoon is produced in Lumbricus weightening finish.
The preferred embodiment of the present invention described in detail above; but, the present invention is not limited to the detail in above-mentioned embodiment, in the technology concept of the present invention; technical scheme can be carried out multiple simple variant, these simple variant belong to protection scope of the present invention.
It is further to note that each the concrete technical characteristic described in above-mentioned detailed description of the invention, in the case of reconcilable, can be combined by any suitable means.In order to avoid unnecessary repetition, various possible compound modes are illustrated by the present invention the most separately.
Additionally, can also carry out combination in any between the various different embodiment of the present invention, as long as it is without prejudice to the thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a Bacillus licheniformis, it is characterised in that: this Bacillus licheniformis (Bacillus
Licheniformis) deposit number is CGMCC No.12316.
2. a microbial bacterial agent, this microbial bacterial agent contains thalline and culture medium, it is characterised in that:
Described thalline contains the Bacillus licheniformis that deposit number is CGMCC No.12316.
Microbial bacterial agent the most according to claim 2, it is characterised in that: every gram of described microorganism
In microbial inoculum, deposit number is that the viable count of the Bacillus licheniformis of CGMCC No.12316 is
108-109cfu。
Microbial bacterial agent the most according to claim 2, it is characterised in that: described thalline also contains
The bacillus thuringiensis that has article number to be ACCC01954 and the rice root that article number is ACCC32592
Mycete, wherein, deposit number is the Bacillus licheniformis of CGMCC No.12316, Su Yun gold spore
The cfu content of bacillus ACCC01954 and Rhizopus oryzae ACCC32592 is than for 1:(0.5-1):
(0.3-0.6)。
Microbial bacterial agent the most according to claim 2, it is characterised in that: described culture medium is cattle
At least one in meat extract protein culture medium, nutrient broth medium and LB culture medium.
6. in Bacillus licheniformis described in claim 1 or claim 2-5 described in any one
Microbial bacterial agent application in processing agricultural wastes, described agricultural wastes are selected from straw, poultry
One or more in feces and livestock culture waste water.
7. in Bacillus licheniformis described in claim 1 or claim 2-5 described in any one
Microbial bacterial agent processes the method for agricultural wastes, it is characterised in that comprise the following steps:
(1) make described microbial bacterial agent mix homogeneously with described agricultural wastes and obtain earthworm cultivation substratum,
Regulate the moisture content of described earthworm cultivation substratum to 50-80 weight %；
Wherein, described agricultural wastes contain straw and livestock culture waste water, relative to 100 weight portion straw
Stalk, the consumption of described livestock culture waste water is 500-1000 weight portion, the consumption of described microbial bacterial agent
For 0.5-5 weight portion, the total nitrogen concentration of described livestock culture waste water is 100-200mg/L；
(2) in described earthworm cultivation substratum, Lumbricus is inoculated, wherein, relative to 100 weight portion straws,
The inoculum density of described Lumbricus is 20-40 bar；
(3) described Lumbricus is made to cultivate 50-90 days in earthworm cultivation substratum, in breeding process, earthworm
Adding livestock culture waste water in earthworm cultivation substrate, addition is 10-30 weight portion/sky；
(4) after breeding process terminates, being separated with wormcast by Lumbricus, Lumbricus is used for livestock culture feedstuff
And pharmacy industry raw material, wormcast plants fertilizer for fruit and vegerable.
Method the most according to claim 7, it is characterised in that described agricultural wastes possibly together with
Fowl and animal excrement, relative to 100 weight portion straws, the consumption of described fowl and animal excrement is 10-50 weight portion.
9. according to the method described in claim 7 or 8, it is characterised in that described straw and manure of livestock and poultry
Just particle diameter is 5-10mm, and the moisture content of described fowl and animal excrement is 6-20%.
Method the most according to claim 7, it is characterised in that described Lumbricus is Eisenia foetida
(Eisenia foetida)。
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479912A (en) * | 2016-09-08 | 2017-03-08 | 江南大学 | The bacillus licheniformis of one plant of cellulase-producing and its application |
| CN106800940A (en) * | 2016-12-30 | 2017-06-06 | 中国农业科学院农业环境与可持续发展研究所 | A kind of soil conditioner and its production and use |
| CN106833668A (en) * | 2016-12-30 | 2017-06-13 | 中国农业科学院农业环境与可持续发展研究所 | A kind of mineral microorganism soil conditioner and its production and use |
| CN107164287A (en) * | 2017-07-12 | 2017-09-15 | 中国农业科学院农业资源与农业区划研究所 | Bacterium bacterial strain, microbial bacterial agent and its application |
| CN107502572A (en) * | 2017-09-08 | 2017-12-22 | 中国农业科学院农业环境与可持续发展研究所 | Application of the bacillus licheniformis in straw degradative, the microbial bacterial agent comprising the bacterium and its application |
| CN108976042A (en) * | 2018-09-14 | 2018-12-11 | 苏州格瑞格登新材料科技有限公司 | A kind of organic fertilizer and preparation method of agricultural wastes degradation |
| CN110835610A (en) * | 2018-08-17 | 2020-02-25 | 吉林农业大学 | Composite microbial inoculum suitable for degrading straw and preparation method thereof |
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| CN115340963A (en) * | 2022-06-16 | 2022-11-15 | 武汉市农业科学院 | Bacillus licheniformis, microecological preparation thereof and application of bacillus licheniformis in agaricus bisporus culture medium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340182A (en) * | 2013-07-22 | 2013-10-09 | 南开大学 | Method of earthworm cultivation through cow dung straw compost |
| CN103834603A (en) * | 2014-03-31 | 2014-06-04 | 金陵科技学院 | Bacillus licheniformis for decomposing feathers of pigeons and application of bacillus licheniformis |
| US20150257391A1 (en) * | 2014-03-14 | 2015-09-17 | Osprey Biotechnics, Inc. | Bacillus licheniformis strain |
| CN105505809A (en) * | 2015-07-21 | 2016-04-20 | 南开大学 | Microbial agent for cleaning oil extraction wastewater filtering membrane |
-
2016
- 2016-05-24 CN CN201610348821.3A patent/CN105886440A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340182A (en) * | 2013-07-22 | 2013-10-09 | 南开大学 | Method of earthworm cultivation through cow dung straw compost |
| US20150257391A1 (en) * | 2014-03-14 | 2015-09-17 | Osprey Biotechnics, Inc. | Bacillus licheniformis strain |
| CN103834603A (en) * | 2014-03-31 | 2014-06-04 | 金陵科技学院 | Bacillus licheniformis for decomposing feathers of pigeons and application of bacillus licheniformis |
| CN105505809A (en) * | 2015-07-21 | 2016-04-20 | 南开大学 | Microbial agent for cleaning oil extraction wastewater filtering membrane |
Non-Patent Citations (1)
| Title |
|---|
| 袁科平，等: "一株水产地衣芽孢杆菌的鉴定及培养基优化", 《福建农林大学学报(自然科学版)》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479912B (en) * | 2016-09-08 | 2019-10-15 | 江南大学 | The bacillus licheniformis of one plant of cellulase-producing and its application |
| CN106479912A (en) * | 2016-09-08 | 2017-03-08 | 江南大学 | The bacillus licheniformis of one plant of cellulase-producing and its application |
| CN106800940A (en) * | 2016-12-30 | 2017-06-06 | 中国农业科学院农业环境与可持续发展研究所 | A kind of soil conditioner and its production and use |
| CN106833668A (en) * | 2016-12-30 | 2017-06-13 | 中国农业科学院农业环境与可持续发展研究所 | A kind of mineral microorganism soil conditioner and its production and use |
| CN107164287A (en) * | 2017-07-12 | 2017-09-15 | 中国农业科学院农业资源与农业区划研究所 | Bacterium bacterial strain, microbial bacterial agent and its application |
| CN107164287B (en) * | 2017-07-12 | 2020-11-20 | 中国农业科学院农业资源与农业区划研究所 | Bacterial strains, microbial agents and uses thereof |
| CN107502572A (en) * | 2017-09-08 | 2017-12-22 | 中国农业科学院农业环境与可持续发展研究所 | Application of the bacillus licheniformis in straw degradative, the microbial bacterial agent comprising the bacterium and its application |
| CN107502572B (en) * | 2017-09-08 | 2020-08-21 | 中国农业科学院农业环境与可持续发展研究所 | Application of bacillus licheniformis in straw degradation, microbial agent containing bacillus licheniformis and application of bacillus licheniformis |
| CN110835610A (en) * | 2018-08-17 | 2020-02-25 | 吉林农业大学 | Composite microbial inoculum suitable for degrading straw and preparation method thereof |
| CN110835610B (en) * | 2018-08-17 | 2022-08-26 | 吉林农业大学 | Composite microbial inoculum suitable for degrading straw and preparation method thereof |
| CN108976042A (en) * | 2018-09-14 | 2018-12-11 | 苏州格瑞格登新材料科技有限公司 | A kind of organic fertilizer and preparation method of agricultural wastes degradation |
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| CN111925964B (en) * | 2020-08-21 | 2022-04-22 | 扬州大学 | Bacillus megaterium and application thereof |
| CN115340963A (en) * | 2022-06-16 | 2022-11-15 | 武汉市农业科学院 | Bacillus licheniformis, microecological preparation thereof and application of bacillus licheniformis in agaricus bisporus culture medium |
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