CN105505809A - Microbial agent for cleaning oil extraction wastewater filtering membrane - Google Patents
Microbial agent for cleaning oil extraction wastewater filtering membrane Download PDFInfo
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- CN105505809A CN105505809A CN201510430186.9A CN201510430186A CN105505809A CN 105505809 A CN105505809 A CN 105505809A CN 201510430186 A CN201510430186 A CN 201510430186A CN 105505809 A CN105505809 A CN 105505809A
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- oil extraction
- filtering membrane
- liquid
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- petroleum hydrocarbon
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Abstract
The invention relates to a microbial agent for cleaning an oil extraction wastewater filtering membrane, and belongs to the field of petroleum pollution treatment. The microbial agent comprises the following steps: preparation of components of a petroleum hydrocarbon degrading strain, preparation of components of a lignocellulose degrading solution, preparation of components of an oxidase solution, preparation of a cleaning solution and the like. According to the invention, a cleaning agent, which is high in cleaning efficiency, broad in application scope, high-temperature resistant, non-toxic and harmless, and environment-friendly, is prepared by virtue of the efficient high-temperature resistant petroleum hydrocarbon degrading strain, namely bacillus licheniformis, as well as bacillus subtilis and a waste fungus pack for high production of cellulase, and a bio-surfactant; and the microbial agent not only can rapidly clean oil stains and dead leaves on the oil extraction wastewater filtering membrane, but also can rapidly and effectively degrade the cleaned stains, so that the reusability of the oil extraction wastewater filtering membrane is improved.
Description
Technical field
The present invention relates to a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane, be a kind of oil extraction waste water filtering membrane clean-out system utilizing bacillus licheniformis agent, bacillus subtilis microbial agent, MICROBIAL SURFACTANT and discarded bacterium bag extracting solution to make specifically, belong to the field of petroleum pollution process.
Background technology
A large amount of oily(waste)water can be produced in oil production process; in process oily(waste)water process; because the higher sewage disposal filter membrane that usually can cause of oil viscosity blocks; thus reduction sewage treating efficiency; and oil attachment makes the cleaning of filter membrane become very difficult for a long time, the cost that poor filter membrane repeat usage causes sewage disposal is higher.
Traditional cleaning oil pollution method mainly contains physics, chemistry and biological washing out method, current chemical cleaning method uses the most extensive, it utilizes pharmaceutical chemicals or other solvents and dirt to react, greasy dirt dissolving, emulsification, dispersion etc. is made to reach the object taken out stains, but the use of some organic solvents easily causes secondary pollution, and the degradation effect of oil is poor, easily pollutes transfer.
MICROBIAL SURFACTANT is at some specific microorganisms oozy meta-bolites with certain surface activity in its metabolic process, as glycolipid, saccharose lipid, lipopeptid or neutral lipid derivative etc.Containing the not only hydrophilic but also material of the group of oleophylic in bio-surfactant, compared to chemical surfactant, huge and the chemical structure of complexity make surfactivity and emulsifying capacity stronger, can fermentative production be typical " green " technique, the characteristic such as hypotoxicity, environmental friendliness, also contributes to oil degradation microbial growth simultaneously.Common MICROBIAL SURFACTANT has rhamnolipid, sophorolipid, marine alga glycolipid etc.
We find in a practical situation, clogging sewage disposal filter membrane not only have oil, also have some withered leaves, because its volume is large and thin, quality is pliable and tough, is not easily cleaned out, so in the cleaning process of filter membrane, need to add some efficient Mierocrystalline celluloses, lignin degradation microbial inoculum, make withered leaf can soften decomposition at short notice.Discarded bacterium bag is the biomass solid wastes produced in cultivating edible process, wherein containing a large amount of laccase, and not only can efficient degradation lignocellulose, also oxidation-degradable petroleum hydrocarbon after extracting.Recycling for discarded bacterium bag provides a new outlet.
Oil degradation bacteria refers to can be the microorganism of single carbon source for growth with petroleum hydrocarbon, both can decompose the baroque alkane of Long carbon chain, also some more difficult aromatic hydrocarbon utilized that are decomposed of degradable, relative to chemical, has and wider utilizes scope.All the time, find the important process that more efficient petroleum hydrocarbon degradation bacterium is petroleum pollution and microorganism field investigator always, and such as low temperature and high temperature can keep the highly active petroleum hydrocarbon degradation bacterium most important thing studied of investigators especially under some extreme conditions.
The lignocellulose degrading bacteria agent (fermentation of bacillus subtilis liquid) of extracting in the present invention, oxydase liquid (discarded bacterium bag extracting solution) are 45 DEG C-55 DEG C through the best degradation temperature of test determination, reach best for making cleaning performance, therefore choose the high temperature resistant oil degradation bacteria of a strain as starting material, simultaneously, higher treatment temp can improve the solubleness of petroleum hydrocarbon in clean-out system further, thus increases cleaning performance.
Summary of the invention
The object of the invention is to: a kind of oil extraction waste water process filter membrane clean-out system utilizing the lichen bacillus ferments liquid, fermentation of bacillus subtilis liquid, bio-surfactant and discarded bacterium bag extracting solution to prepare is provided.
The present invention seeks to be achieved through the following technical solutions: a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane, is characterized in that: comprise the steps:
(1) preparation of petroleum hydrocarbon degradation bacterium: petroleum hydrocarbon degradation bacterium is inoculated in LB liquid nutrient medium, in air table 40 DEG C, 120r/min cultivates 5000r/min centrifugal segregation supernatant liquor after 3 days, bacterial sediment is for subsequent use;
(2) preparation of ligocellulose degradation's liquid: cellulose-degrading bacteria is inoculated in and produces enzyme liquid nutrient medium, in air table 30 DEG C, that 120r/min cultivates 5000r/min after 3 days is centrifugal, getting supernatant liquor 50 DEG C, to be concentrated into 1/10 volume for subsequent use;
(3) preparation of oxydase liquid: discarded bacterium bag is put into inorganic salt solution and soak 5 days, by for subsequent use after soak solution filtering precipitation;
(4) the oxydase liquid will extracted in the ligocellulose degradation's liquid extracted in the petroleum hydrocarbon degradation bacterium extracted in inorganic salt solution, step (1), step (2), step (3), is mixed in proportion;
(5) by the mixture in step (4) in air table 45 DEG C, 120r/min add after cultivating 12 hours a certain proportion of tensio-active agent and mixing namely make clean-out system;
In aforesaid method, in step (1), petroleum hydrocarbon degradation bacterium used is Bacillus licheniformis (BacilluslicheniformisY-1), is separated obtains in 2012 in the contaminated soil of oil producing region, Dagang Oilfield, Tianjin City,
preservationin China General Microbiological bacterial classification
preservationadministrative center,
preservationbe numbered CGMCCNo.11019,
preservationdate is on June 26th, 2015.Preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
In aforesaid method, in step (2), cellulose-degrading bacteria used is bacillus subtilis (BacillussubtilisNH11), obtains in 2011 in Jia Liming vermiculture field, Tianjin, existing
preservationin Nankai University's biomass solid wastes recycling center.
In aforesaid method, it is the discarded bacterium bag going out more than three damp mushrooms in mushroom plant that edible mushrooms used discards bacterium bag, can use the discarded bacterium bag of the edible mushrooms such as flat mushroom, mushroom, chicken mushroom, Coprinus comatus.
In aforesaid method, in step (3), step (4), inorganic salt solution formula used is: Nacl30g, NH
4nO
31g, KH
2pO
40.5g, K
2hPO
41g, MgSO
40.5g, CaCl
20.2g, liquid microelement 1ml, distilled water 1L, pH7.0,121 DEG C of sterilizing 20min.
In aforesaid method, in step (4), the configuration proportion of each component is inorganic salt solution: petroleum hydrocarbon degradation bacterium: ligocellulose degradation's liquid: oxydase liquid is 8: 1: 1: 3.
In aforesaid method, the tensio-active agent added in step (5) can be rhamnolipid, sophorolipid, marine alga glycolipid, if adding rhamnolipid addition is 0.5%, sophorolipid is 1%, marine alga glycolipid is 1%.
The present invention compared with prior art has the following advantages:
1. treatment effect is good, has clear up ability more by force to complicated dirt.
The present invention utilizes multiple bacteria agent to make a kind of efficient cleaner, has comparatively strong cleaning and degradation capability to the oil on oil extraction waste water process filter membrane, withered leaf.
2. be applicable to pyroprocessing, strengthen the cleaning ability of clean-out system.
Present invention utilizes the high temperature resistant oil degradation bacteria of a strain, under the treatment condition of 45 DEG C, not only reach the best degradation temperature of cellulose degradation microbial inoculum used, lignin degradation microbial inoculum, petroleum hydrocarbon degradation bacterium used also can keep comparatively Seedling height activity and petroleum hydrocarbon degradation ability, at this temperature simultaneously, oil on filter membrane is compared normal temperature process and can better be dissolved in clean-out system, improves emulsifying effectiveness, thus reaches higher dirt degradation rate.
3. through the cleaning of clean-out system of the present invention, the dirt on filter membrane can reach higher degradation efficiency, reduces the pollutant load of discharge, and this clean-out system material therefor is microbial fermentation product, without hazardous and noxious substances, accomplishes that environment is more friendly.
Embodiment
Embodiment 1:
(1) petroleum hydrocarbon degradation bacterium is inoculated in LB liquid nutrient medium, in air table 40 DEG C, 120r/min cultivates 5000r/min centrifugal segregation supernatant liquor after 3 days, bacterial sediment is for subsequent use;
(2) lignocellulose degrading bacteria be inoculated in produce enzyme liquid nutrient medium, in air table 30 DEG C, that 120r/min cultivates 5000r/min after 3 days is centrifugal, getting supernatant liquor 50 DEG C, to be concentrated into 1/10 volume for subsequent use;
(3) discarded bacterium bag is put into inorganic salt solution to soak 5 days, by for subsequent use after soak solution filtering precipitation;
(4) will for inorganic salt solution, petroleum hydrocarbon degradation bacterium, ligocellulose degradation's liquid, oxydase liquid in 8: 1: 1: 3 ratio mixing, then in air table 45 DEG C, 120r/min cultivate 12 hours after add 0.5% rhamnolipid and mixing namely make clean-out system;
Cleaning performance is checked:
Getting after 30cm dries containing pollutent filter membrane weigh (M1) adds in the triangular flask that 200ml scavenging solution is housed, 45 DEG C, 120r/min process 3 days, with the thalline of distilled water clean surface attachment after filter membrane is taken out, put into oven for drying and weigh (M2), remove completely to pollutent with the careful cleaning filter membranes of methylene dichloride, weigh after oven dry (M3) again.Pollutants removal rate Q calculation formula is as follows:
After process in 3 days, pollutants removal rate reaches 92%.
Embodiment 2:
(1) petroleum hydrocarbon degradation bacterium is inoculated in LB liquid nutrient medium, in air table 40 DEG C, 120r/min cultivates 5000r/min centrifugal segregation supernatant liquor after 3 days, bacterial sediment is for subsequent use;
(2) lignocellulose degrading bacteria be inoculated in produce enzyme liquid nutrient medium, in air table 30 DEG C, that 120r/min cultivates 5000r/min after 3 days is centrifugal, getting supernatant liquor 50 DEG C, to be concentrated into 1/10 volume for subsequent use;
(3) discarded bacterium bag is put into inorganic salt solution to soak 5 days, by for subsequent use after soak solution filtering precipitation;
(4) will for inorganic salt solution, petroleum hydrocarbon degradation bacterium, ligocellulose degradation's liquid, oxydase liquid in 8: 1: 1: 3 ratio mixing, then in air table 45 DEG C, 120r/min cultivate 12 hours after add 1% sophorolipid and mixing namely make clean-out system;
After process in 3 days, pollutants removal rate reaches 95%.
Claims (7)
1. for cleaning a microbiobacterial agent for oil extraction waste water filtering membrane, it is characterized in that, method comprises the steps:
(1) preparation of petroleum hydrocarbon degradation bacterium: petroleum hydrocarbon degradation bacterium is inoculated in LB liquid nutrient medium, in air table 40 DEG C, 120r/min cultivates 5000r/min centrifugal segregation supernatant liquor after 3 days, bacterial sediment is for subsequent use;
(2) preparation of ligocellulose degradation's liquid: lignocellulose degrading bacteria is inoculated in and produces enzyme liquid nutrient medium, in air table 30 DEG C, that 120r/min cultivates 5000r/min after 3 days is centrifugal, getting supernatant liquor 50 DEG C, to be concentrated into 1/10 volume for subsequent use;
(3) preparation of oxydase liquid: discarded bacterium bag is put into inorganic salt solution and soak 5 days, by for subsequent use after soak solution filtering precipitation;
(4) the oxydase liquid will extracted in the ligocellulose degradation's liquid extracted in the petroleum hydrocarbon degradation bacterium extracted in inorganic salt solution, step (1), step (2), step (3), is mixed in proportion;
(5) by the mixture in step (4) in air table 45 DEG C, 120r/min add after cultivating 12 hours a certain proportion of tensio-active agent and mixing namely make clean-out system.
2. a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane as described in right 1, it is characterized in that, in step (1), petroleum hydrocarbon degradation bacterium used is Bacillus licheniformis (BacilluslicheniformisY-1), be separated in the contaminated soil of oil producing region, Dagang Oilfield, Tianjin City in 2011 and obtain, be now preserved in institute of microbiology of the Chinese Academy of Sciences (CGMCCNo.11019).
3. a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane as described in right 1, it is characterized in that, in step (2), lignocellulose degrading bacteria used is bacillus subtilis (BacillussubtilisNH11), obtained in Jia Liming vermiculture field, Tianjin in 2011, be now preserved in Nankai University's biomass solid wastes recycling center.
4. a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane as described in right 1, it is characterized in that, it is the discarded bacterium bag going out more than three damp mushrooms in mushroom plant that edible mushrooms used discards bacterium bag, can use the discarded bacterium bag of the edible mushrooms such as flat mushroom, mushroom, chicken mushroom, Coprinus comatus.
5. a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane as described in right 1, is characterized in that, in step (3), step (4), inorganic salt solution formula used is: Nacl30g, NH
4nO
31g, KH
2pO
40.5g, K
2hPO
41g, MgSO
40.5g, CaCl
20.2g, liquid microelement 1ml, distilled water 1L, pH7.0,121 DEG C of sterilizing 20min.
6. a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane as described in right 1, it is characterized in that, in step (4), the configuration proportion of each component is inorganic salt solution: petroleum hydrocarbon degradation bacterium: ligocellulose degradation's liquid: oxydase liquid is 8: 1: 1: 3.
7. a kind of microbiobacterial agent for cleaning oil extraction waste water filtering membrane as described in right 1, it is characterized in that, the tensio-active agent added in step (5) can be rhamnolipid, sophorolipid, marine alga glycolipid, if adding rhamnolipid addition is 0.5%, sophorolipid is 1%, marine alga glycolipid is 1%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886440A (en) * | 2016-05-24 | 2016-08-24 | 中国农业科学院农业环境与可持续发展研究所 | Bacillus licheniformis and microbial agent and application and method of microbial agent in treatment of agricultural wastes |
| CN106975364A (en) * | 2016-12-29 | 2017-07-25 | 浙江海洋大学 | A kind of method that composite petroleum degradation bacteria cleans oily pollution milipore filter |
| CN107470348A (en) * | 2017-09-28 | 2017-12-15 | 无锡盛雅生物科技有限公司佛山分公司 | A kind of microorganism formulation for petroleum-contaminated soil reparation |
| CN108271776A (en) * | 2017-12-25 | 2018-07-13 | 武汉科诺生物科技股份有限公司 | Bacillus lipopeptid class metabolite is used as the purposes of microbial bacteria agent aid |
| CN111672471A (en) * | 2020-06-22 | 2020-09-18 | 陈苗根 | Biological activated carbon capable of improving COD removal efficiency and preparation method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886440A (en) * | 2016-05-24 | 2016-08-24 | 中国农业科学院农业环境与可持续发展研究所 | Bacillus licheniformis and microbial agent and application and method of microbial agent in treatment of agricultural wastes |
| CN106975364A (en) * | 2016-12-29 | 2017-07-25 | 浙江海洋大学 | A kind of method that composite petroleum degradation bacteria cleans oily pollution milipore filter |
| CN106975364B (en) * | 2016-12-29 | 2019-06-28 | 浙江海洋大学 | A kind of method of composite petroleum degradation bacteria cleaning oily pollution ultrafiltration membrane |
| CN107470348A (en) * | 2017-09-28 | 2017-12-15 | 无锡盛雅生物科技有限公司佛山分公司 | A kind of microorganism formulation for petroleum-contaminated soil reparation |
| CN108271776A (en) * | 2017-12-25 | 2018-07-13 | 武汉科诺生物科技股份有限公司 | Bacillus lipopeptid class metabolite is used as the purposes of microbial bacteria agent aid |
| CN111672471A (en) * | 2020-06-22 | 2020-09-18 | 陈苗根 | Biological activated carbon capable of improving COD removal efficiency and preparation method and application thereof |
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Application publication date: 20160420 |