CN106964644B - organic matter soil remediation agent, preparation method and application thereof in sludge digestion - Google Patents

Abstract

The invention provides an organic matter soil remediation agent, a preparation method and application thereof in sludge digestion, and adopts the technical scheme that: the method is used for carrying out innocent treatment on the urban sewage sludge in the early stage. After the sludge repairing agent is used for 1-6 months through experiments, the proportion effective removal rate of various pollutants in the soil reaches more than 95%, and the soil meets the national standard. Meanwhile, the fermentation product can acidify hardened saline-alkali soil and is also a good organic fertilizer, which is beneficial to agricultural production and reduces the use of chemical reagents and fertilizers, and avoids pollution sources and secondary pollution from the source.

Description

Organic matter soil remediation agent, preparation method and application thereof in sludge digestion

Technical Field

the invention relates to an organic soil remediation agent, in particular to a method for preparing the organic soil remediation agent by using microorganism treated sludge, the obtained organic soil remediation agent and application thereof in sludge digestion.

background

the pollution caused by toxic organic matters is particularly serious in harm caused in recent years, and a plurality of organic matters belong to carcinogenic, teratogenic and mutagenic substances. The U.S. data show that 117 of the 2211 organic compounds examined in various bodies of water are considered or suspected carcinogens. Among the priority controlled pollutants regulated by the U.S. Environmental Protection Agency (EPA), the organic compounds account for 88% of the total, and are all toxic organic compounds. The toxic organic matters mainly comprise various organic pesticides, organic pigments and dyes, aromatic amine, polycyclic aromatic hydrocarbon and the like, and the main sources of the toxic organic matters are organic chemical wastewater of coking, dyes, pesticides, medicines, plastic synthesis and the like. Most of the toxic organic pollutants have larger molecules and more complex structures, are not easy to biodegrade and are not easy to remove in biological treatment and natural environment, so that the pollution caused by the toxic organic matters is concerned by the nation, environmental protection experts and vast people. Since the 20 th century and 80 th century, with the development of industrialization and globalization, the production center of toxic organic matters is shifted from europe and america to asia and other developing countries with cheap labor force and underdeveloped economy, and china is the transfer center of industrial enterprises with high water consumption and serious pollution for the first time. However, the treatment rate of the wastewater is very low in China, most of the wastewater is directly discharged without treatment or after simple treatment, natural water bodies which human beings rely on for survival are polluted, human health is harmed, and environmental pollution and ecological damage are more and more serious.

In the current method for treating municipal sludge in China, the fixed fermentation tank turning and throwing type composting is a widely applied process. The method has the advantages of less investment of fixed assets, low equipment operation cost, easy matching and the like, and although the method has the defects of long fermentation period, large occupied area, low treatment efficiency and the like, the method still continues to exert the advantages of the method because the urban sewage treatment plant is generally built in the suburbs and the land use problem is not outstanding. In the fixed fermentation tank turning and throwing type composting process, sawdust, straw powder, rice hulls and the like are required to be used as conditioning agents, and the conditioning agents can absorb water, fluffy and dredge oxygen. But the compost cannot play a role in supporting and dredging oxygen all the time because of self decomposition and collapse in the composting process. Therefore, the conditioner only consisting of the wood chips, the straw powder, the rice hulls and the like can only play a transient role, and gradually loses the conditioning function in the middle and later periods of the compost; thereby affecting the treatment effect of sludge compost, prolonging the composting time, increasing the sludge treatment cost and further affecting the recycling of sludge resources.

The soil is a basic resource on which human beings rely to live, and is an important place for the material, energy exchange and circulation of the earth ecosystem. Once contaminated, the environmental impact is persistent and irreversible. The soil environment quality and ecological safety are important guarantees of sustainable development, with the development of industrial and agricultural industries, the soil environment of China is increasingly worsened, soil resources bear more and more pressure, and at present, the cultivated land area polluted by heavy metal of China is nearly 2000 hectares, and occupies about 1/5 of the total cultivated land area. The land polluted by the mining area reaches 200 million hectares, the land polluted by petroleum is about 500 million hectares, the solid waste is piled up and pollutes about 5 million hectares, the farmland polluted by the industrial three wastes is nearly 1000 million hectares, and the farmland area irrigated by sewage reaches 330 more than ten thousand hectares. Soil pollution causes the reduction of agricultural grain in China to be over 1300 million tons, and economic loss caused by pesticide and organic matter pollution, radioactive pollution, pathogenic bacteria pollution and other types of pollution is difficult to estimate. Due to pollution, the nutritive function, purification function, buffering function of soil and supporting function of organisms are being lost, and remediation and treatment are urgently required. The research and application of the generation process and regulation of the polluted soil and the remediation of the polluted soil are deeply developed, the direction of the innovation of the remediation technology of the polluted soil is tightly held, and the ecological safety of China is directly concerned.

Disclosure of Invention

in order to overcome the difficulty of the conventional sludge digestion, the sludge organic matter polluted soil remediation agent not only solves the sludge disposal problem, but also changes waste into valuable, changes the sludge into the soil remediation agent and can restore the soil polluted by organic matters.

the technical problem of the sludge organic matter polluted soil remediation agent is solved by adopting the following technical scheme: the heavy metal treatment is carried out on the municipal sewage sludge at the early stage, the dehydration treatment is carried out to ensure that the water content is about 40 percent, and the main component of the sludge is organic matter which is suitable for culturing bacterial flora. Thermophilic bacteria are used for decomposing organic matters and can effectively kill pathogenic bacteria. Mixed culture of Ideonella sakaiensis flora, acetic acid flora, high efficiency degrading flora and silicic acid flora. The bacterial flora is powerful and can eliminate harmful bacteria again. The synthesized high-efficiency degradation bacterium group continuously generates an oxidant in the using process, quickly degrades organic pollutants through an advanced oxidation way, improves soil texture, promotes water and oxygen transfer and pollutant mass transfer, and enhances biodegradation. Meanwhile, the loaded high-efficiency degradation flora is prepared by screening, separating, enriching and optimizing the proportion of different organic pollutants (such as gasoline, diesel oil, crude oil and the like), different series of the high-efficiency degradation flora are suitable for degrading pollutants such as plastic, gasoline, diesel oil, crude oil and the like, and the high-efficiency degradation flora has the characteristics of strong pertinence and stable community function. Compared with the traditional repairing agent, the repairing agent has the characteristics of high oxidation efficiency, convenient application and good safety.

the biological combination effect can quickly realize the reduction of the toxicity and the total removal of the pollutants. Compared with other technologies, the method has the advantages of high removal efficiency, basically 6 to 10 months of restoration time, basically no damage to soil matrix, low treatment cost and the like. Is suitable for treating organic pollutants. Can be repaired in various modes such as in situ or ex situ.

The sludge organic pollution soil repairing agent comprises: the organic sludge is suitable for being cultured as a basic material for culturing Ideonella sakaiensis flora and high-efficiency degradation flora in the process of decomposition, dissolution, adsorption, synthesis and conversion. The cultured high-efficiency bacterial flora degrades organic pollutants. After 180 days of test use of the sludge repairing agent, the content of the organic pollutants in the soil with medium and light organic pollutants is lower than the international standard, and the soil meets the national soil standard of China. And simultaneously, partial acid substances are generated to acidify hardened saline-alkali soil. The organic fertilizer is good, is beneficial to agricultural production, reduces the use of chemical reagents and fertilizers, and avoids pollution sources and secondary pollution from the source.

One of the purposes of the invention is to provide an organic matter soil remediation agent, and the preparation method of the organic matter soil remediation agent comprises the following steps;

1. Sludge pretreatment

The domestic sludge produced by the urban domestic sewage treatment plant is stacked and aired in an airing field for 10-15 days, and a biological deodorant can be properly added. The odor is reduced primarily, the machine is dehydrated, and the water content is reduced from about 75% to below 40%.

Adding adjuvants into the dried sludge, mixing the above components, pulverizing with a pulverizer or a crusher, and sieving to obtain sludge granules with particle size of less than 10 mm.

The auxiliary materials have the following functions: the water content, the air permeability and the cation exchange capacity of the sludge are adjusted or the humic acid content of the sludge can be increased. In order to improve the fermentation efficiency, the mass part ratio of the auxiliary materials to the sludge can be 1: 20-100;

the auxiliary materials can be one or any combination of crushed straws, wheat straws, pomace, bean dregs, rice chaff, shale, turf, sawdust, coconut husk or manioc waste. Wherein the straws, the wheat straws, the pomace, the bean dregs and the rice chaff can provide a carbon source and a nitrogen source; sawdust, cassava residue or coconut husk can adjust the water content and air permeability of sludge, shale can adjust the cation exchange capacity of sludge, and turf can increase the humic acid content of sludge and adjust the cation exchange capacity of sludge. For example, it may be selected from: the straw comprises the following components in parts by mass: shale, turf and sawdust in the weight ratio of 1-1.5 to 1-1.5; bean dregs: shale, turf and coconut husk which are 1: (1-1.5: 1-1.5); fruit residue leftovers of a fruit juice factory: shale, turf and cassava dregs (1-1.5), 1-1.5, etc.

2. preparation of compound microbial agent

(1) Preparing a composite microorganism liquid preparation: the composite microbial preparation for efficiently degrading organic waste comprises Bacillus stearothermophilus (Bacillus stearothermophilus), Myceliophthora thermophilus (Myceliophthora thermophila), Aspergillus carbonarius (Aspergillus carbonarius), white rot fungi (Phanerochaete chrysosporium), Penicillium heterosporum (Penicillium subvatum), Pseudomonas putida (Pseudomonas putida), Alcaligenes faecalis (Alcaligenes faecalis), Bacillus subtilis (Bacillus subtilis) and Bacillus thuringiensis (Bacillus), wherein the composite microbial preparation comprises Bacillus stearothermophilus (Bacillus stearothermophilus) with a bacterial count of 10-25% and Myceliophthora thermophilus with a bacterial count of 10-20% and Aspergillus carbonarius with a bacterial count of 10-25% and Bacillus thermophilus with a bacterial count of 10-20% and Aspergillus carbonarius with a bacterial count of 10-20% respectively, the percentage of the number of the bacteria of the Penicillium heterosporum (Penicillium divaricatum) to the total number of the bacteria is 5-15%, the percentage of the number of the bacteria of the pseudomonas putida (Pseudomonas putida) to the total number of the bacteria is 10-20%, the percentage of the number of the bacteria of the Alcaligenes faecalis (Alcaligenes faecalis) to the total number of the bacteria is 5-15%, the percentage of the number of the bacteria of the Bacillus subtilis to the total number of the bacteria is 10-20%, the percentage of the number of the bacteria of the Bacillus thuringiensis (Bacillus) to the total number of the bacteria is 10-20%, and the Penicillium heterosporum (Penicillium divaricatum) is prepared into seed liquid by using a conventional biological method;

The above-mentioned strains used in the present invention are conventional strains in the art, and may be purchased from the market, or from the China general microbiological culture Collection center (CGMCC) and the American Type Culture Collection (ATCC).

(2) Adsorbing the composite microorganism liquid preparation in an organic mixture optionally composed of zeolite powder, straw, bean pulp, fruit residue, grass peat and the like to obtain the composite microorganism liquid preparation; preferably, the weight parts of the components are as follows: 2-4 parts of composite microorganism liquid preparation, 10-60 parts of straw or 40-60 parts of zeolite powder or 30-50 parts of soybean meal or 5-15 parts of fruit residues or 10-15 parts of grass carbon; the proportion of the specific adsorption material to the composite microorganism liquid preparation can be adjusted according to specific requirements and purposes.

3. fermentation of composite microbial agent

Inoculating the composite microbial inoculum obtained in the step 2) into the pretreated sludge raw material, stirring by using a mixer, and mixing the composite microbial inoculum with the pretreated raw material according to the weight ratio of 1: uniformly mixing the raw materials in a ratio of 100-1000;

Meanwhile, nutrient salt solution is inoculated, the inoculation amount of the nutrient salt solution is 5-50g/1000kg of sludge, and each liter of the nutrient salt solution comprises (NH4)2SO4 and 1-15 g; KH2PO4, 1-5 g; k2HPO4 & 3H2O, 1-5 g; 0.01-0.1 g of CaCl 2; 0.1-1 mg of FeSO 4; 1-15 g of yeast powder; MgCl 2.6H 2O, 1-1.5 g; 0.1-5 g of cysteine;

Mixing the above materials, and stirring; then transporting the prepared sludge to a fermentation workshop for composting fermentation;

The temperature of the fermentation workshop is normal temperature (25-35 ℃), the temperature of the sludge reaches above 50 ℃ after 2 days of fermentation, the sludge is maintained above 50 ℃ for 3 days, the sludge is turned over once every 3 days, the sludge is fermented for 14 days, the sludge is basically thoroughly decomposed, the odor disappears, the temperature is reduced to below 40 ℃, and the water content is reduced to below 35%.

4. preparation of organic matter degrading bacteria group

(1) preparing organic matter degrading bacteria: the organic matter degrading bacteria group contains degrading bacteria group, acetic acid bacteria group and silicic acid bacteria group, wherein,

the degrading flora is a commercial bacterial agent for degrading macromolecules, and is selected from: ideonella sakaiensis flora, Pseudomonas stutzeri (with the preservation number of CGMCC No.1738), Citrobacter faeri (CCTCC NO: M208004), Bacillus (Bacillus), Alcaligenes (Acaigines), Flavobacterium (Flavobacterium), Arthrobacter (Arthrobacter), Micrococcus (Micrococcus), etc.;

the acetic acid bacteria group is commercially available acetic acid bacteria agent selected from Acinetobacter, Juniperus, purple bacillus, Bizhi bacteria, lactic acid bacteria, Bacillus, acetic acid bacteria, Actinomycetes, yeast, Clostridium, etc.;

The silicate flora is a commercially available silicate microbial inoculum selected from bacillus subtilis, bacillus mucilaginosus and the like,

the above-mentioned strains used in the present invention are conventional strains in the art, and may be purchased from the market, or from the China general microbiological culture Collection center (CGMCC) and the American Type Culture Collection (ATCC).

The organic matter degrading bacteria group comprises 50-80% of degrading bacteria group, 20-40% of acetic acid bacteria group and 30-50% of silicic acid bacteria group.

The preparation method comprises the following steps:

Firstly, preparing degradation flora, acetic acid flora and silicic acid flora into corresponding seed liquid by utilizing a conventional biological means, adding a proper amount of commercially available LB fermentation liquid or conventional fermentation liquid into a microbial fermentation tank system, fermenting for 3-5 days at a constant liquid temperature of 25-30 ℃, and mixing in proportion.

(2) strain solidification

Taking wheat bran, straws and other materials as an attachment carrier of microorganisms, inoculating the culture solution in the step (1) onto the attachment carrier according to the weight ratio of 1: 10-20, piling the uniformly stirred materials into a conical pile, introducing natural wind into the pile, fermenting for 4-10 days, and naturally drying to form a powdery substance.

Before use, activating organic matter degrading bacteria with warm water at 10-40 deg.C, storing in water to form biodegradable bacteria liquid, wherein the bacteria liquid is activated with water of 8-10L for 0.5-2.0 hr, and the bacteria liquid is prepared by mixing the above materials, adding water, and sterilizing.

The invention also provides an application of the organic soil remediation agent in sludge digestion, which comprises the following specific steps:

Uniformly stirring 80-100 parts by weight of the sludge fermented in the step (3) and 40-50 parts by weight of activated organic matter degrading bacteria, uniformly spreading and spraying the mixture in soil polluted by organic matter, and ploughing the soil to fully mix the mixture and the polluted soil; the thickness is 30 cm, the area is 100 square meters of treatment area, 1 kg-2 kg of solid powdery organic matter degrading bacteria needs to be added, and 200 kg-500 kg of sludge fermented in the step (3) needs to be added. For the soil with serious pollution, the dosage can be increased according to the specific requirements.

meanwhile, supplementing water and carrying out plowing and oxygen exposure on the soil, wherein when in-situ bioremediation of the soil is carried out, the thickness of the restored soil is controlled within 30 centimeters, and the soil is plowed once in one month after inoculation on average in one week; and ploughing once in two weeks, and adding water at any time to keep the water content of the soil above 30%, wherein the added water does not contain a bactericide component.

The soil remediation agent prepared by the invention has the beneficial effects that:

1. The invention adopts wheat bran, straw and other solidified high-efficiency macromolecular degrading bacteria, solidifies degrading strains on a porous loose material, dries the degrading strains to enable microorganisms to exist in a dormant state, and can obtain the required active microorganisms only by activating the microorganisms with warm water when in use. The problem of long-term storage of microorganisms is solved, and meanwhile, materials such as wheat bran and straws can provide good air permeability and effective carbon sources for the microorganisms in the subsequent soil remediation process.

2. the microbial soil remediation agent disclosed by the invention has the advantages that beneficial microbes are applied to soil, the microbial plot system of the soil is optimized, the physical properties of the soil are improved, the hardened and degenerated soil is remedied, the biological activity of the soil is enhanced, and the metabolism of crops is regulated; promoting the growth of crops, degrading and transforming pollutants in soil and inhibiting soil-borne diseases; enhancing the stress resistance of crops, increasing the yield, improving the quality and being ecological and environment-friendly.

3. The biological surfactant is widely applied to improvement and treatment of saline-alkali soil aiming at the current situation that organic matter polluted soil is easy to harden into blocks to form a part of anaerobic environment, has the characteristic of improving the soil, introduces a soil conditioner which is more fluffy and difficult to harden into a biological repair technology, increases the air permeability of the soil, creates a good environment for degradation reaction of microorganisms, and simultaneously is added with various nutrients, particularly contains a small amount of amino acid required by the growth of the microorganisms to form the biological surfactant, so that the biological surfactant also has the effects of improving the activity of the microorganisms and promoting the growth of the microorganisms.

Detailed Description

the soil remediation agent, the preparation method and the application thereof in sludge digestion according to the present invention will be described in detail with reference to the following embodiments. The measurement method of each technical index of the sludge used in the experiment is carried out according to the agricultural industry standard (NY884-2004) of the people's republic of China.

Drawings

FIG. 1 is a field test curve of bioremediation of land with 7% and 20% oil content by using the product of the invention

Example 1 preparation of organic soil remediation agent

1. Sludge pretreatment

The domestic sludge produced by the urban domestic sewage treatment plant is stacked and aired in an airing field for 10-15 days, and a biological deodorant can be properly added. The odor is reduced primarily, the machine is dehydrated, and the water content is reduced from about 75% to below 40%.

Adding auxiliary materials into the dried sludge according to the mass ratio of 1: 50, wherein the auxiliary materials are a mixture of crushed straws, wheat straws, pomace, bean dregs, rice chaff and sawdust.

the components are uniformly mixed and crushed by a crusher or a crusher, and are sieved to prepare sludge granules with the grain size of less than 10mm for later use.

The auxiliary materials have the following functions: the water content, the air permeability and the cation exchange capacity of the sludge are adjusted or the humic acid content of the sludge can be increased.

2. Preparation of compound microbial agent

(1) Preparing a composite microorganism liquid preparation: the composite microbial preparation for efficiently degrading organic waste of the present invention comprises Bacillus stearothermophilus (Bacillus stearothermophilus), Myceliophthora thermophilus (Myceliophthora thermophila), Aspergillus carbonarius (Aspergillus carbonarius), white rot fungus (Phanerochaete chrysosporium), Penicillium heterosporum (Penicillium subvermim), Pseudomonas putida (Pseudomonas putida), Alcaligenes faecalis (Alcaligenes faecalis), Bacillus subtilis (Bacillus subtilis) and Bacillus thuringiensis (Bacillus thuringiensis), wherein the composite microbial preparation comprises Bacillus stearothermophilus (Bacillus stearothermophilus) having a bacterial count of 10% and Myceliophthora thermophilus having a bacterial count of 10% and Aspergillus carbonarius having a bacterial count of 10% and Bacillus thermophilus having a bacterial count of 10% and Aspergillus niger having a carbon black count of 10% in total, and the composite microbial preparation comprises Aspergillus thermophilus having a bacterial count of 10% and Aspergillus niger having a carbon black count of 10% in total Myceliophthora Bacillus thermophilus (Aspergillus thermophilus) of 10% in total fungal count of 10% and Aspergillus niger having a carbon black count of 10% in total Myceliophthora Bacillus thermophilus (Aspergillus niger content of 10% in total Myceliophthora Bacillus subtilis), the percentage of the number of the pseudomonas putida (pseudomonas putida) to the total number of the bacteria is 20 percent, the percentage of the number of the Alcaligenes faecalis (Alcaligenes faecalis) to the total number of the bacteria is 5 percent, the percentage of the number of the Bacillus subtilis to the total number of the bacteria is 10 percent, the percentage of the number of the Bacillus thuringiensis (Bacillus) to the total number of the bacteria is 10 percent, and the pseudomonas putida (pseudomonas putida) is prepared into seed liquid by using a conventional biological method;

The above-mentioned strains used in the present invention are conventional strains in the art, and may be purchased from the market, or from the China general microbiological culture Collection center (CGMCC) and the American Type Culture Collection (ATCC).

(2) Adsorbing the composite microorganism liquid preparation in an organic mixture consisting of zeolite powder, straws, bean pulp and fruit residues to obtain the composite microorganism liquid preparation; preferably, the weight parts of the components are as follows: 4 parts of composite microorganism liquid preparation, 10 parts of straw and 30 parts of soybean meal.

3. Fermentation of composite microbial agent

Inoculating the composite microbial inoculum obtained in the step 2) into the pretreated sludge raw material, stirring by using a mixer, and mixing the composite microbial inoculum with the pretreated raw material according to the weight ratio of 1: mixing uniformly according to the proportion of 100;

meanwhile, nutrient salt solution is added, the adding amount of the nutrient salt solution is 10g/1000kg of sludge, and each liter of the nutrient salt solution comprises (NH4)2SO4 and 1-15 g; KH2PO4, 1-5 g; k2HPO4 & 3H2O, 1-5 g; 0.01-0.1 g of CaCl 2; 0.1-1 mg of FeSO 4; 1-15 g of yeast powder; MgCl 2.6H 2O, 1-1.5 g; 0.1-5 g of cysteine;

Mixing the above materials, and stirring; then transporting the prepared sludge to a fermentation workshop for composting fermentation;

the temperature of the fermentation workshop is normal temperature (25-35 ℃), the temperature of the sludge reaches above 50 ℃ after 2 days of fermentation, the sludge is maintained above 50 ℃ for 3 days, the sludge is turned over once every 3 days, the sludge is fermented for 14 days, the sludge is basically thoroughly decomposed, the odor disappears, the temperature is reduced to below 40 ℃, and the water content is reduced to below 35%.

4. Preparation of organic matter degrading bacteria group

(1) preparing organic matter degrading bacteria: the organic matter degrading bacteria group contains degrading bacteria group, acetic acid bacteria group and silicic acid bacteria group, wherein the degrading bacteria group is pseudomonas stutzeri (the preservation number is CGMCC No.1738), the acetic acid bacteria group is commercially available acinetobacter, and the silicic acid bacteria group is commercially available silicate bacteria. The degrading flora accounts for 50% of the total amount of the organic matter degrading flora, the acetic acid flora accounts for 20% of the total amount of the organic matter degrading flora, and the silicic acid flora accounts for 30% of the total amount of the organic matter degrading flora.

The preparation method comprises the following steps:

firstly, preparing degradation flora, acetic acid flora and silicic acid flora into corresponding seed liquid by utilizing a conventional biological means, adding a proper amount of commercially available LB fermentation liquid or conventional fermentation liquid into a microbial fermentation tank system, fermenting for 3-5 days at a constant liquid temperature of 25-30 ℃, and mixing in proportion.

(2) strain solidification

Taking wheat bran, straws and other materials as an attachment carrier of microorganisms, inoculating the culture solution in the step (1) onto the attachment carrier according to the weight ratio of 1:10, piling the materials after uniformly stirring into a conical pile, introducing natural wind into the pile, and naturally drying after fermenting for 7 days to form powdery substances.

example 2 in situ remediation of soil

activating the organic matter degrading bacteria group with warm water at 30 deg.c and maintaining in water to form biodegradable bacteria liquid in the ratio of 1Kg strain, activating with 8L water and soaking for 2.0 hr.

and (3) adding 1kg of solid powdery organic matter degrading bacteria and 200 kg of sludge fermented in the step (3) to a treatment area with the thickness of 30 cm and the area of 100 square meters. The activated flora and the fermented sludge are uniformly stirred, the mixture is uniformly spread and sprayed in the soil polluted by organic matters, and the soil is ploughed to fully mix the mixture with the polluted soil.

Meanwhile, supplementing water and carrying out plowing and oxygen exposure on the soil, wherein when in-situ bioremediation of the soil is carried out, the thickness of the restored soil is controlled within 30 centimeters, and the soil is plowed once in one month after inoculation on average in one week; and ploughing once in two weeks, and adding water at any time to keep the water content of the soil above 30%, wherein the added water does not contain a bactericide component.

the test results show that: for the soil with the oil content of 7% (W/W), after the soil is degraded for one month, the oil content in the soil can reach below 3%; after two months, the oil content in the soil can be reduced to below 0.8 percent; after three months, the oil content in the soil can be reduced to below 0.5 percent, and the mineral oil content index in the national pollutant control standard GB4284-84 in agricultural sludge is reached.

Meanwhile, other contaminants (pentachlorophenol and phenol) in the soil were detected as shown in table 1.

example 3 in situ remediation of soil

as described in example 2, except that the degree of soil contamination was different, 2 kg of solid powdery organic matter-degrading bacteria and 400 kg of sludge after fermentation in step (3) were used

The test results show that: for the soil with the oil content of 20% (W/W), after the soil is degraded for one month, the oil content in the soil is below 6%; after two months, the oil content in the soil is 2.5 percent, and the degradation speed is slow; after four months, the oil content in the soil reaches below 1.5 percent.

The test curve is shown in figure 1, and the bioremediation field test curve using the product of the invention.

Meanwhile, other contaminants (pentachlorophenol and phenol) in the soil were detected as shown in table 1.

TABLE 1 Effect of remediation Agents on contaminant degradation

(3) after the saline-alkali soil is repaired for 1 year by applying the soil repairing agent prepared in example 1, the soil of the planted field is detected, and the results are shown as follows:

TABLE 2 soil index after 1 year of remediation with soil remediation Agents

as can be seen from the table 2, after the soil remediation agent prepared by the method is used for 1 year, the soil of a planting area is obviously improved, the ventilation gap of the soil is increased by 35-40%, the granular structure of the soil is increased by 43-55%, the total phosphorus content of the soil is increased by 50-52%, and the total nitrogen content of the soil is increased by 34-36%.

the invention is not limited to the foregoing embodiments. The invention extends to any novel feature or any novel combination of features disclosed in this specification and any novel method or process steps or any novel combination of features disclosed.

Claims (4)

1. A preparation method of an organic matter soil remediation agent is characterized by comprising the following steps: 1) pretreating a sludge raw material; 2) preparing a compound microbial agent; 3) fermenting the compound microbial agent; 4) preparing organic matter degrading bacteria; wherein, the sludge pretreatment comprises the following steps:

Stacking and airing domestic sludge generated by an urban domestic sewage treatment plant in an airing field for 10-15 days, and properly adding a biological deodorant; preliminarily reducing the odor, dehydrating the machine and reducing the water content from 75% to below 40%;

Adding auxiliary materials into the dried sludge, uniformly mixing all the components, crushing by using a crusher or a crusher, and screening to prepare sludge granules with the particle size of less than 10mm for later use;

the mass part ratio of the auxiliary material to the sludge is 1: 20-100;

The auxiliary materials are one or any combination of crushed straws, wheat straws, pomace, bean dregs, rice chaff, shale, turf, sawdust, coconut husk or manioc waste; selected from: the straw comprises the following components in parts by mass: shale, turf and sawdust in the weight ratio of 1-1.5 to 1-1.5; bean dregs: shale, turf and coconut husk which are 1: (1-1.5: 1-1.5); fruit residue leftovers of a fruit juice factory: shale, turf and cassava dregs (1-1.5), 1-1.5 and 1-1.5 respectively; the preparation of the compound microbial agent comprises the following steps:

(1) Preparing a composite microorganism liquid preparation: the composite microbial preparation for degrading organic waste, which contains Bacillus stearothermophilus (Bacillus stearothermophilus), Myceliophthora thermophilus (Myceliophthora thermophila), Aspergillus carbonarius (Aspergillus carbonarius), white rot fungus (Phanerochaete chrysosporium), Penicillium heterophyticum (Penicillium diversum), Pseudomonas putida (Pseudomonas putida), Alcaligenes faecalis (Alcaligenes faecalis), Bacillus subtilis (Bacillus subtilis) and Bacillus thuringiensis (Bacillus), wherein the composite microbial preparation contains Bacillus stearothermophilus (Bacillus stearothermophilus) in a percentage of 10-25% and Myceliophthora thermophilus in a percentage of 10-20% and Aspergillus carbonarius (Aspergillus carbonarius) in a percentage of 10-20% and Aspergillus carbonarius in a percentage of 10-20% in a percentage of the total number of the fungi, and the composite microbial preparation contains Bacillus stearothermophilus (Aspergillus thermophilus) in a thermophilus in a percentage of 10-20% and Aspergillus carbonarius in a percentage of 5-20% in a total number of the Bacillus thermophilus, the percentage of the number of the bacteria of the Penicillium heterosporum (Penicillium divaricatum) to the total number of the bacteria is 5-15%, the percentage of the number of the bacteria of the pseudomonas putida (Pseudomonas putida) to the total number of the bacteria is 10-20%, the percentage of the number of the bacteria of the Alcaligenes faecalis (Alcaligenes faecalis) to the total number of the bacteria is 5-15%, the percentage of the number of the bacteria of the Bacillus subtilis to the total number of the bacteria is 10-20%, the percentage of the number of the bacteria of the Bacillus thuringiensis (Bacillus) to the total number of the bacteria is 10-20%, and the Penicillium heterosporum (Penicillium divaricatum) is prepared into seed liquid by using a conventional biological method;

The adopted strains are conventional strains in the field, and are purchased from the market or from the China general microbiological culture Collection center (CGMCC) and the American type culture Collection center (ATCC);

(2) Adsorbing the composite microorganism liquid preparation in an organic mixture optionally composed of zeolite powder, straw, bean pulp, fruit residue and grass carbon to obtain the composite microorganism liquid preparation; the weight parts of each component are as follows: 2-4 parts of composite microorganism liquid preparation, 10-60 parts of straw or 40-60 parts of zeolite powder or 30-50 parts of soybean meal or 5-15 parts of fruit residues or 10-15 parts of grass carbon; the proportion of the specific adsorption material and the composite microorganism liquid preparation is adjusted according to specific requirements and purposes;

Wherein, the fermentation of the compound microbial agent is as follows:

Inoculating the composite microbial inoculum obtained in the step 2) into the pretreated sludge raw material, stirring by using a mixer, and mixing the composite microbial inoculum with the pretreated raw material according to the weight ratio of 1: uniformly mixing the raw materials in a ratio of 100-1000;

Meanwhile, nutrient salt solution is inoculated, the inoculation amount of the nutrient salt solution is 5-50g/1000kg of sludge, and each liter of the nutrient salt solution comprises (NH4)2SO4 and 1-15 g; KH2PO4, 1-5 g; k2HPO4 & 3H2O, 1-5 g; 0.01-0.1 g of CaCl 2; 0.1-1 mg of FeSO 4; 1-15 g of yeast powder; MgCl 2.6H 2O, 1-1.5 g; 0.1-5 g of cysteine;

Mixing the above materials, and stirring; then transporting the prepared sludge to a fermentation workshop for composting fermentation;

The temperature of a fermentation workshop is 25-35 ℃, the temperature of the sludge reaches above 50 ℃ after 2 days of fermentation, the sludge is maintained above 50 ℃ for 3 days, the sludge is turned over once every 3 days, the sludge is fermented for 14 days, the odor disappears, the temperature is reduced to below 40 ℃, and the water content is reduced to below 35%; wherein, the organic matter degradation bacterial group is prepared as follows:

(1) preparing organic matter degrading bacteria: the organic matter degrading bacteria group contains degrading bacteria group, acetic acid bacteria group and silicic acid bacteria group, wherein,

the degrading flora is a commercial bacterial agent for degrading macromolecules, and is selected from: ideonella sakaiensis flora, pseudomonas stutzeri with the preservation number of CGMCC No.1738, and Citrobacter faeri CCTCC NO: m208004, Bacillus (Bacillus), Alcaligenes (Acaligenes), Flavobacterium (Flavobacterium), Arthrobacter (Arthrobacter), Micrococcus (Micrococcus);

The acetic acid bacteria group is commercially available acetic acid bacteria agent selected from Acinetobacter, Juniperus, purple bacillus, Bizhi bacteria, lactic acid bacteria, Bacillus, acetic acid bacteria, Actinomycetes, yeast, and Clostridium;

The silicate flora is a commercially available silicate microbial inoculum selected from Bacillus subtilis and Bacillus mucilaginosus,

the adopted strains are conventional strains in the field, and are purchased from the market or from the China general microbiological culture Collection center (CGMCC) and the American type culture Collection center (ATCC);

The organic matter degrading bacteria comprise 50-80% of degrading bacteria, 20-40% of acetic acid bacteria and 30-50% of silicic acid bacteria;

The preparation method comprises the following steps:

respectively preparing degradation flora, acetic acid flora and silicic acid flora into corresponding seed liquid by utilizing a conventional biological means, adding a proper amount of commercially available LB fermentation liquid or conventional fermentation liquid into a microbial fermentation tank system, fermenting for 3-5 days at a constant liquid temperature of 25-30 ℃, and mixing in proportion;

(2) Strain solidification

Taking wheat bran and straw materials as attachment carriers of microorganisms, inoculating the culture solution in the step (1) onto the attachment carriers according to the weight ratio of 1: 10-20, piling the materials after uniformly stirring into a conical pile, introducing natural wind into the pile, fermenting for 4-10 days, and naturally drying to form powdery substances.

2. An organic soil remediation agent characterized in that it is prepared by the method of claim 1.

3. Use of the organic soil remediation agent obtained in claim 2, wherein said use is:

Before use, activating the organic matter degrading bacteria group with warm water at 10-40 deg.c and storing in water to form 1Kg of bacteria strain, activating with 8-10L of water for 0.5-2.0 hr;

uniformly stirring 80-100 parts by weight of the sludge fermented in the step (3) and 40-50 parts by weight of activated organic matter degrading bacteria, uniformly spreading and spraying the mixture in soil polluted by organic matter, and ploughing the soil to fully mix the mixture and the polluted soil; the thickness is 30 cm, the area is 100 square meters of treatment area, 1 kg-2 kg of solid powdery organic matter degrading bacteria needs to be added, and 200 kg-500 kg of sludge fermented in the step (3) needs to be added; for the seriously polluted soil, the dosage is increased according to the specific requirement;

Meanwhile, supplementing water and carrying out plowing and oxygen exposure on the soil, wherein when in-situ bioremediation of the soil is carried out, the thickness of the restored soil is controlled within 30 centimeters, and the soil is plowed once in one month after inoculation on average in one week; and ploughing once in two weeks, and adding water at any time to keep the water content of the soil above 30%, wherein the added water does not contain a bactericide component.

4. a method of sludge digestion using the organic soil remediation agent of claim 2.