CN107900096A - Based on compost reinforcing laccase by persistent organism contaminated soil restorative procedure - Google Patents
Based on compost reinforcing laccase by persistent organism contaminated soil restorative procedure Download PDFInfo
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- CN107900096A CN107900096A CN201711058856.4A CN201711058856A CN107900096A CN 107900096 A CN107900096 A CN 107900096A CN 201711058856 A CN201711058856 A CN 201711058856A CN 107900096 A CN107900096 A CN 107900096A
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- compost
- contaminated soil
- laccase
- soil
- persistent organism
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
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Abstract
The present invention relates to it is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.Its technical solution is：Compost is added in the contaminated soil to be repaired by persistent organism, the addition of the compost is 5.5 ~ 18wt% to be repaired by persistent organism contaminated soil, obtains adding the contaminated soil of compost；Then the laccase of 400 ~ 500U, the soil in being repaired are added in the contaminated soil that compost is added described in every Kg；The moisture content of the soil in the reparation is adjusted again to 35 ~ 40%, is stirred evenly, 3 ~ 7d of holding, described to be repaired by persistent organism contaminated soil in atmosphere.The persistent organism is one or more of nitrogen heterocyclic, polycyclic aromatic hydrocarbon and Polychlorinated biphenyls；The compost is one or more of garden waste compost, excrement of animals compost, sludge composting.The present invention has the characteristics that repairing efficiency is short, repairing effect is good and non-secondary pollution.
Description
Technical field
The invention belongs to by persistent organism contaminated soil recovery technique field.It is strong based on compost more particularly to one kind
Change laccase by persistent organism contaminated soil restorative procedure.
Background technology
With the development of the social economy, the various pollutants produced in human industry or agricultural production process pass through different ways
Footpath enters soil environment, eventually exceeds soil self purification activity, soil quality is changed with function.And soil or agricultural production
Product and some biological source of nutrition, pollutant can occur to transmit by food chain and migration, finally jeopardizes the mankind and other lifes
The survival and development of thing.Organic contaminants in soil such as nitrogen heterocyclic (NHCs), polycyclic aromatic hydrocarbon (PAHs) and Polychlorinated biphenyls
(PCBs), because poisonous, difficult degradation, being not easy to be destroyed by metabolic process, and carcinogenic, teratogenesis, mutagenic property are subject to environment
The attention of worker.
NHCs is the heterocycle structure organic compound containing nitrogen-atoms in molecule, belong to the wide, toxicity of pollution it is larger one
Class hardly degraded organic substance, is widely present in soil and waste water, and common NHCs mainly has pyrroles, indoles, pyridine, quinoline, different
Quinoline and its derivates etc., since it is difficult to biodegradation, frequently results in conventional biological treatment system treatment effect and not enough manage
Think.PAHs is volatile hydrocarbon caused by organic matter imperfect combustion, and the fast development of modern age industrial or agricultural causes
PAHs is finally largely converged in soil and underground water with approach such as Wet deposition, sewage irrigations, causes serious soil
Earth and underground water pollution, are important environment and food contaminant.PCBs is that a kind of artificial synthesized chlorinated aromatic hydrocarbons are lasting
Property organic pollution, is high hydrophobic compound, has highly lipophilic, bioconcentration, chemical stability and half volatile, be easy to
Circulate in the environment, the PCBs that main loop process is related to contaminated site is spread to air volatilization, through air long-distance sand transport simultaneously
Soil and water body environment are entered by dried wet deposition.Therefore, the recovery technique for studying persistent organism in soil is agreed with currently
The demand of soil pollution reparation, has important practical significance.
In recent years, soil pollution reparation has been to be concerned by more and more people.Enzyme recovery technique is directly specific using certain
The degradation capability purifying contaminated soil of inoculating microbe enzyme.Compared with traditional physico-chemical process, enzyme recovery technique has effect
Rate is high, environmental-friendly, non-secondary pollution and it is easy to operate the advantages that.Pollution amelioration is directly carried out than microbiological treatment and plant using enzyme
Thing processing is more advantageous.Such as enzyme is of less demanding to nutrition condition in environment；Some degradable specific pollutants of enzyme.But
Enzyme recovery technique also has some limitations：Enzyme can be degraded by Soil protease；Enzyme or unstable and easy in inactivation；Enzyme can be by soil
Protease is degraded and unstable and easy in inactivation.
Laccase is a kind of glycoprotein, using molecular oxygen by free radical catalytic reaction mechanism come the various phenols of catalysis oxidation and
Non-phenolic compounds.By being inserted into mediator, the non-aldehydes matter of laccase catalysis oxidation can be enable, the research to mediator shows：
All there is the structural features of N-OH in most non-natural mediators, and exactly N-OH can be under the action of laccase
Free radical is generated, attack macromolecule substrate or non-phenolic substances are then gone by the free radical of generation.Some researches show that laccase
Repairing NHCs, PAHs, PCBs class contaminated soil has good treatment effect, but there is also some problems, such as mediator material
Expensive, stability difference and processing not exclusively (reparation [D] of Bao Xin laccases mediator systems to nitrogen-containing heterocycle compound polluted-water
Wuhan University Of Technology, 2013) (Zhang Yuanyuan .EK repair the feasibility study of PAHs contaminated soils and laccase as EK joint technology
[D] Wuhan University Of Technologies, 2014) (Korinna Kordon.Oxidative dehalogenation of chlorinated
hydroxybiphenyls by laccases of white-rot fungi[J].International
Biodeterioration&Biodegradation.2010)。
Compost is to utilize various plant residues (crop straw, weeds, leaf, peat, rubbish and other discarded objects etc.)
Or the organic fertilizer for wanting raw material to be formed through composting decomposition based on people and animals' fecaluria, contained nutriment is relatively abundant, and fertilizer efficiency it is long and
Stablize, while may advantageously facilitate the formation of soil solid particless structure.Meanwhile humic acid is rich in compost, this is the weight of compost fertility
Want source.Humic acid is the larger molecular organics matter being widely present in nature, and molecular structure is sufficiently complex, contains various functions
Base, wherein mainly oxygen containing acid function base, including carboxyl and phenolic hydroxyl group on aromatic series and aliphatic compound.Humic acid
There are a certain number of free radicals on molecule, there is physiological activity.The binding ability of humic acid and enzyme is stronger, the phase between them
Interaction has protective effect to the activity and stability of enzyme.In view of the property of compost, has using compost repairing polluted soil
Report.But current, numerous studies all concentrate on processing heavy metal field, and (an Xiang sludge organism charcoal base compost is to soil polluted by manganese
The influence of property and its reparation [J] agro-environment science journals, 2015) (Chen Han pine compost rehabilitating soil metallic pollution researchs
Be in progress [J] applications and environmental, and 2008), the report using compost treatment organic pollution is actually rare, Dong Fen (Dong
Fragrant compost degrading polycyclic aromatic hydrocarbons contaminated soil remediation technical research [D] Beijing University of Chemical Technology, 2012) and aftershock (aftershock pentachloro-s
The compost reparation of phenol contaminated soil and its microbiologic population's composition research [D] Hunan University, 2012) it have studied respectively at compost
Polycyclic aromatic hydrocarbon pollution and pentachlorophenol contaminated soil are managed, but all there are long processing period and incomplete problem of degrading, and
Also there are the possibility that intermediate product continues contaminated soil.
The content of the invention
It is contemplated that overcome prior art defect, it is therefore an objective to provide that a kind of repairing efficiency is short, repairing effect is good and without two
Secondary pollution based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.
To achieve the above object, the technical solution adopted by the present invention is：It is to be repaired by persistent organism pollution
Add compost in soil, the addition of the compost for it is to be repaired by the 5.5 of persistent organism contaminated soil~
18wt%, obtains adding the contaminated soil of compost；Then described in every Kg add compost contaminated soil in add 400~
The laccase of 500U, the soil in being repaired；The moisture content of the soil in the reparation is adjusted again to 35~40%, and stirring is equal
It is even, 3~7d is kept in atmosphere, it is described to be repaired by persistent organism contaminated soil.
The laccase is the laccase of white-rot fungi Pleurotus sp. secretions, and enzyme activity is 3~4U/mL；The white-rot fungi
Pleurotus sp. belong to the fungi of Basidiomycotina.
The persistent organism is one or more of nitrogen heterocyclic, polycyclic aromatic hydrocarbon and Polychlorinated biphenyls.
The compost is by except impurity, the organic fertilizer for air-drying and smashing to pieces；The compost is garden waste compost, livestock
One or more of excrement composting, sludge composting.
As a result of such scheme, the present invention has the following advantages：
(1), the present invention using laccase reparation by persistent organism contaminated soil, can in several days fast degradation it is dirty
Thing is contaminated, it is efficient；Paint enzyme viability determines that the final product of pollutant can be H2O and CO2, it is environmental-friendly, it will not cause secondary
Pollution.
(2), the present invention uses compost, can not only increase soil water-retaining, insulation, the ventilative, ability of fertilizer conservation, to a certain degree
The upper fertility for improving soil, fast quick-recovery soil agricultural functional, and the quantity and activity of edaphon can also be improved, and show
The activity for improving the original laccase of soil is write, is that pollutant obtains more completely degrading.
(3), the present invention uses compost, and humic acid therein provides enough freedom for laccase degraded persistent organism
Base, the presence of the hydroxyl on its molecular structure also cause humic acid become laccase degrade non-phenols persistent organism Jie
Body.The stability and activity of protective enzyme are also capable of in interaction between humic acid and laccase, slow down laccase activity decrease speed,
The time of laccase effect is greatly increased, is significantly improved by the repairing effect of persistent organism contaminated soil.
Therefore, the present invention has the characteristics that repairing efficiency is short, repairing effect is good and non-secondary pollution.
Embodiment
With reference to embodiment, the invention will be further described, not to the limitation of its protection domain.
In present embodiment：
The laccase is the laccase of white-rot fungi Pleurotus sp. secretions, and enzyme activity is 3~4U/mL；The white-rot fungi
Pleurotus sp. belong to the fungi of Basidiomycotina.
The compost is by except impurity, the organic fertilizer for air-drying and smashing to pieces；The compost is garden waste compost, livestock
One or more of excrement composting, sludge composting.
Repeated no more in embodiment.
Embodiment 1
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.Held to be repaired
Add compost in the soil of long property organic pollution, the addition of the compost is to be to be repaired by persistent organism pollution
5.5~14wt% of soil, obtains adding the contaminated soil of compost；Then the contaminated soil of compost is being added described in every Kg
The laccase of 400~460U of middle addition, the soil in being repaired；Adjust again the moisture content of the soil in the reparation to 35~
38%, stir evenly, keep 3~5d in atmosphere, it is described to be repaired by persistent organism contaminated soil.
The persistent organism is polycyclic aromatic hydrocarbon.
Embodiment 2
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.The present embodiment is except described
Persistent organism is outside the mixture of nitrogen heterocyclic and Polychlorinated biphenyls.Remaining is the same as embodiment 1.
Embodiment 3
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.The present embodiment is except described
Persistent organism is outside the mixture of nitrogen heterocyclic, polycyclic aromatic hydrocarbon and Polychlorinated biphenyls.Remaining is the same as embodiment 1.
Embodiment 4
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.Held to be repaired
Add compost in the soil of long property organic pollution, the addition of the compost is to be to be repaired by persistent organism pollution
8~16wt% of soil, obtains adding the contaminated soil of compost；Then added described in every Kg in the contaminated soil of compost
Add the laccase of 440~480U, the soil in being repaired；Adjust again the moisture content of the soil in the reparation to 36~
39%, stir evenly, keep 4~6d in atmosphere, it is described to be repaired by persistent organism contaminated soil.
The persistent organism is nitrogen heterocyclic.
Embodiment 5
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.The present embodiment is except described
Persistent organism is outside the mixture of polycyclic aromatic hydrocarbon and Polychlorinated biphenyls.Remaining is the same as embodiment 4.
Embodiment 6
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.The present embodiment is except described
Persistent organism is outside the mixture of nitrogen heterocyclic, polycyclic aromatic hydrocarbon and Polychlorinated biphenyls.Remaining is the same as embodiment 4.
Embodiment 7
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.Held to be repaired
Add compost in the soil of long property organic pollution, the addition of the compost is to be to be repaired by persistent organism pollution
10~18wt% of soil, obtains adding the contaminated soil of compost；Then the contaminated soil of compost is being added described in every Kg
The laccase of 460~500U of middle addition, the soil in being repaired；Adjust again the moisture content of the soil in the reparation to 37~
40%, stir evenly, keep 5~7d in atmosphere, it is described to be repaired by persistent organism contaminated soil.
The persistent organism is Polychlorinated biphenyls.
Embodiment 8
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.The present embodiment is except described
Persistent organism is outside the mixture of nitrogen heterocyclic and polycyclic aromatic hydrocarbon.Remaining is the same as embodiment 7.
Embodiment 9
It is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure.The present embodiment is except described
Persistent organism is outside the mixture of nitrogen heterocyclic, polycyclic aromatic hydrocarbon and Polychlorinated biphenyls.Remaining is the same as embodiment 7.
Present embodiment has the following advantages：
(1), present embodiment using laccase reparation by persistent organism contaminated soil, can be fast in several days
Fast degradation of contaminant, it is efficient；Paint enzyme viability determines that the final product of pollutant can be H2O and CO2, it is environmental-friendly, will not
Cause secondary pollution.
(2), present embodiment uses compost, can not only increase soil water-retaining, insulation, the ventilative, ability of fertilizer conservation,
In a way improve soil fertility, fast quick-recovery soil agricultural functional, and can also improve edaphon quantity and
Activity, and the activity of the original laccase of soil is significantly improved, it is that pollutant obtains more completely degrading.
(3), present embodiment uses compost, and humic acid therein provides enough for laccase degraded persistent organism
Free radical, the presence of the hydroxyl on its molecular structure also causes humic acid to become laccase and degrade the persistent organism of non-phenols
Mediator.The stability and activity of protective enzyme are also capable of in interaction between humic acid and laccase, slow down laccase activity decline
Speed, greatly increases the time of laccase effect, significantly improves by the repairing effect of persistent organism contaminated soil.
Therefore, present embodiment has the characteristics that repairing efficiency is short, repairing effect is good and non-secondary pollution.
Claims (4)
1. it is a kind of based on compost strengthen laccase by persistent organism contaminated soil restorative procedure, it is characterised in that to be repaired
Multiple is added compost in persistent organism contaminated soil, the addition of the compost is to be repaired organic by persistence
5.5 ~ 18wt% of thing contaminated soil, obtains adding the contaminated soil of compost；Then the dirty of compost is being added described in every Kg
Contaminate the laccase that 400 ~ 500U is added in soil, the soil in being repaired；The moisture content of the soil in the reparation is adjusted again extremely
35 ~ 40%, stir evenly, keep 3 ~ 7d in atmosphere, it is described to be repaired by persistent organism contaminated soil.
2. it is according to claim 1 based on compost strengthen laccase by persistent organism contaminated soil restorative procedure,
It is characterized in that the laccase is white-rot fungiPleurotus sp.The laccase of secretion, enzyme activity are 3 ~ 4U/mL；The white rot is true
BacteriumPleurotus sp.Belong to the fungi of Basidiomycotina.
3. it is according to claim 1 based on compost strengthen laccase by persistent organism contaminated soil restorative procedure,
It is characterized in that the persistent organism is one or more of nitrogen heterocyclic, polycyclic aromatic hydrocarbon and Polychlorinated biphenyls.
4. it is according to claim 1 based on compost strengthen laccase by persistent organism contaminated soil restorative procedure,
It is characterized in that the compost is by except impurity, the organic fertilizer for air-drying and smashing to pieces；The compost is garden waste compost, domestic animal
Raise one or more of excrement composting, sludge composting.
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| CN201711058856.4A CN107900096A (en) | 2017-11-01 | 2017-11-01 | Based on compost reinforcing laccase by persistent organism contaminated soil restorative procedure |
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| CN201711058856.4A CN107900096A (en) | 2017-11-01 | 2017-11-01 | Based on compost reinforcing laccase by persistent organism contaminated soil restorative procedure |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112778061A (en) * | 2020-12-30 | 2021-05-11 | 北京北方节能环保有限公司 | Alkaline wastewater polluted soil composting additive in TNT refining process and preparation method thereof |
| CN114105714A (en) * | 2021-12-15 | 2022-03-01 | 桂润环境科技股份有限公司 | Soil remediation agent, preparation method and application thereof, and soil remediation method |
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| CN103911295A (en) * | 2014-03-25 | 2014-07-09 | 山东省科学院能源研究所 | Trametes hirsute strain and application thereof in production of laccase by using municipal dewatered sludge |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112778061A (en) * | 2020-12-30 | 2021-05-11 | 北京北方节能环保有限公司 | Alkaline wastewater polluted soil composting additive in TNT refining process and preparation method thereof |
| CN114105714A (en) * | 2021-12-15 | 2022-03-01 | 桂润环境科技股份有限公司 | Soil remediation agent, preparation method and application thereof, and soil remediation method |
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