CN113751495B - Soil remediation agent for chlorophenol pollution - Google Patents
Soil remediation agent for chlorophenol pollution Download PDFInfo
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- CN113751495B CN113751495B CN202111037328.7A CN202111037328A CN113751495B CN 113751495 B CN113751495 B CN 113751495B CN 202111037328 A CN202111037328 A CN 202111037328A CN 113751495 B CN113751495 B CN 113751495B
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Abstract
The invention discloses a soil remediation agent for chlorophenol pollution, and belongs to the technical field of soil remediation. The composite material comprises the following raw materials in parts by weight: activated carbon: 50-80 parts of ethyl acetate: 100-200 parts of ferric chloride: 65-75 parts of Tris: 20-40 parts of hydrogen peroxide: 10-15 parts of white rot fungus freeze-dried powder: 0.01 to 0.05 portion; the preparation method comprises the following steps: (1) preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air-drying, adding pure water, ferric chloride and Tris, heating, fully stirring and uniformly mixing, and drying at low temperature; (2) preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon. The prepared soil remediation agent can effectively improve the degradation rate of chlorophenol.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to a soil remediation agent for chlorophenol pollution.
Background
The chlorophenols are phenols having at least one chlorine atom attached to the benzene ring. They are widely existed in waste water and waste residue produced in the production of dye, pesticide, herbicide, antiseptic, bactericide and antibiotic medicine, etc., and also contain a large amount of over-standard chlorophenol pollutant waste water in the important chemical production process of petrochemical industry, paper making, etc., and when the drinking water containing phenols is treated by using chlorine gas, the chlorophenol compounds can be produced. Since chlorophenols organic pollutants can destroy the protein structure of microorganisms, damage cells and are difficult to be degraded by soil microorganisms, along with the transmission of a food chain, the chlorophenols organic pollutants are accumulated in a human body in large quantities.
The polluted soil restoration technology mainly comprises three main categories of physical, chemical and biological restoration according to the scientific field, wherein the biological restoration has 3 advantages, the first is that the restorer adopted by the biological restoration is plants, animals, microorganisms and the like, the plants, the animals, the microorganisms and the like are all from the nature, the environment-friendly characteristic is realized, and the secondary pollution to the environment is avoided. And secondly, bioremediation of the polluted soil is carried out under the mild conditions of normal temperature and normal pressure, and the method has the characteristic of safety. Thirdly, the cost of bioremediation is low, the bioremediation strain is easy to obtain, and the culture condition is simple. Since the bioremediation has the above 3 characteristics, people are receiving more and more attention.
Disclosure of Invention
1. Problems to be solved
Aiming at the problems in the prior art, the invention provides a soil remediation agent for chlorophenol pollution, which can effectively improve the degradation rate of chlorophenol.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
the soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
the soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
the soil remediation agent prepared by the invention utilizes the modified activated carbon to be mixed with the specific white rot fungi, so that the white rot fungi can be adhered to the surface of the activated carbon, can be planted and can form a biological film, and meanwhile, as can be seen by a scanning electron microscope, after the activated carbon is treated by ferric chloride, ethyl acetate and other substances, the number of pore diameters on the surface is increased, the proportion of pores is large, and the surface is rough, so that the growth and metabolism of the white rot fungi embedded in the soil can be promoted.
Drawings
FIG. 1 is a graph showing the results of treatment of activated carbon in the present invention;
FIG. 2 is an infrared spectrum before and after the modification treatment of the activated carbon in the present invention.
Detailed Description
The invention is further described with reference to specific examples.
Example 1
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Example 2
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Example 3
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Example 4
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Example 5
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Comparative example 1
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the finished product;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Comparative example 2
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
The purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Comparative example 3
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
65 parts of active carbon, namely 65 parts of active carbon,
0.03 part of white rot fungus freeze-dried powder.
In the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is purchased from Rach biotechnology limited company, and the brand is SHBCC, the number of the brand is U.S. A. (NDM3-2), and the Latin chemical name is Phanerochaetc chrysosporium.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Comparative example 4
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus freeze-dried powder is obtained from the following reference documents (Wangwenfeng white rot fungus bioremediation research on polycyclic aromatic hydrocarbon and chlorophenol-polluted soil [ D ]. Huazhong university of science and technology, 2016), and is numbered as Pleurotus sp.CQ 3.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
In the soil remediation agent for chlorophenol pollution, the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
In the soil remediation agent for chlorophenol pollution, the culture medium for biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
Comparative example 5
The soil remediation agent for chlorophenol pollution comprises the following raw materials in parts by weight:
in the soil remediation agent for chlorophenol pollution, the activated carbon is coconut shell activated carbon, the average particle size of the activated carbon is 0.45mm, and the average specific surface area of the activated carbon is 3200m2In g, the pores have an average size of 4 nm.
In the soil remediation agent for chlorophenol pollution, the purity of the ethyl acetate is 99.0%, and the ethyl acetate is purchased from chemical reagents of national drug group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
In the soil remediation agent for chlorophenol pollution, the Tris is purchased from Sigma Aldrich trade company, and the purity of the Tris is 99.9%;
the mass concentration of the hydrogen peroxide is 15%.
In the soil remediation agent for chlorophenol pollution, the preparation method of the soil remediation agent comprises the following steps:
modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the activated carbon.
In the soil remediation agent for chlorophenol pollution, the temperature of hydrogen peroxide in the step (1) is controlled below 8 ℃;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
Example 6
The soil remediation agents prepared in examples 1-5 and comparative examples 1-5 were tested as follows:
(1) the activated carbons prepared in example 5, comparative example 1 and comparative example 3 were selected for electron scanning treatment as shown in fig. 1, wherein a corresponds to the activated carbon treated in example 5, wherein B corresponds to the activated carbon treated in comparative example 1, and wherein C corresponds to the activated carbon prepared in comparative example 3. Specifically, the activated carbon corresponding to C in fig. 1 is not treated, and it can be seen that the activated carbon corresponding to B in fig. 2 is not treated with ferric chloride, and has a few large pores and medium pores, and the activated carbon corresponding to a in fig. 1 has the largest number of micro pores and is densely distributed.
(2) The following documents (wangwang. white rot fungus bioremediation research on polycyclic aromatic hydrocarbon and chlorophenol-contaminated soil [ D ] science and technology university in china, 2016.) are referenced, and o-chlorophenol, a common pesticide raw material, is selected as a research object.
Firstly, preparing soil containing o-chlorophenol, weighing 30g of naturally air-dried soil into a 250mL triangular flask, then uniformly adding 10mL o-chlorophenol n-hexane solution into the triangular flask, and placing the triangular flask in a fume hood for overnight after the triangular flask is opened. And after n-hexane is completely volatilized, obtaining the pollutant soil containing 0.3mg/g o-chlorophenol. The prepared soil remediation agent is buried into contaminated soil containing o-chlorophenol, wherein the input amount of the soil remediation agent is 1g/100g of the contaminated soil, and three soil remediation agents are arranged in parallel in each example or comparative example. Standing and culturing at 25 deg.C for 7 days, extracting pollutants from soil, and measuring its degradation rate. It should be noted that the soil was mixed according to the ratio of 3: adding ethanol extract (ethanol: water: 1) at a ratio of 10, shaking at a constant temperature of 150r/min for 24h, performing ultrasonic extraction for 20min, and taking out supernatant; adding the equal volume of isopropanol extract into the soil again, performing ultrasonic extraction for 20min, and mixing the supernatants for 2 times; the combined supernatant was centrifuged at 10000r/min for 5min, concentrated to 100mL by rotary evaporation, filtered through a 0.2 μm pore size filter and used for HPLC analysis. It should be reminded that o-chlorophenol determination is mandated in the Guangdong province analytical testing technology public laboratory.
TABLE 1 degradation rate of chlorophenol in soil
| Group of | Degradation Rate (%) |
| Example 1 | 80.2 |
| Example 2 | 80.7 |
| Example 3 | 79.4 |
| Example 4 | 80.5 |
| Example 5 | 81.1 |
| Comparative example 1 | 71.6 |
| Comparative example 2 | 74.5 |
| Comparative example 3 | 62.8 |
| Comparative example 4 | 67.0 |
| Comparative example 5 | 12.5 |
In combination with the content of table 1, compared with the comparative example, the soil remediation agent prepared in the embodiment of the invention utilizes the modified activated carbon to mix with the specific white rot fungi, so that the white rot fungi can adhere to and colonize the surface of the activated carbon and form a biological membrane, and meanwhile, as can be seen by a scanning electron microscope, after the activated carbon is treated by ferric chloride, ethyl acetate and other substances, the number of pore diameters on the surface is increased, the proportion of pores is large, and the surface is rough, so that the growth and metabolism of the white rot fungi embedded in the soil are promoted.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (9)
1. A soil remediation agent for chlorophenol-contaminated soil, which is characterized in that:
the composite material comprises the following raw materials in parts by weight:
50 to 80 portions of active carbon,
100 to 200 portions of ethyl acetate, 100 portions of ethyl acetate,
65-75 parts of ferric chloride,
20 to 40 portions of Tris, 20 to 40 portions,
10 to 15 parts of hydrogen peroxide solution,
0.01-0.05 part of white rot fungi freeze-dried powder;
the preparation method of the soil remediation agent comprises the following steps:
(1) modification treatment of activated carbon:
preparing activated carbon, washing with deionized water, repeatedly leaching with hydrogen peroxide, adding ethyl acetate for soaking, air drying, adding pure water, ferric chloride and Tris, heating to 60 ℃, fully stirring and uniformly mixing, and drying at low temperature to obtain the active carbon;
(2) and (3) culturing thallus:
preparing a culture solution, carrying out shake culture on the white rot fungus freeze-dried powder, centrifuging after the shake culture to obtain a supernatant, putting the modified activated carbon obtained in the step (1), continuously stirring to carry out biofilm attachment culture on the activated carbon, and centrifuging to obtain precipitated activated carbon.
2. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
the composite material comprises the following raw materials in parts by weight:
60 to 70 portions of active carbon, namely,
130 to 170 portions of ethyl acetate, and the like,
68 to 72 parts of ferric chloride,
25 to 35 portions of Tris (Tris-Tris),
10 to 15 parts of hydrogen peroxide solution,
0.01-0.05 part of white rot fungi freeze-dried powder.
3. The soil remediation agent for chlorophenol-contaminated soil according to claim 2, wherein:
the composite material comprises the following raw materials in parts by weight:
65 parts of active carbon, namely 65 parts of active carbon,
150 parts of ethyl acetate,
70 parts of ferric chloride, namely, ferric chloride,
30 parts of Tris (sodium chloride, potassium chloride),
13 parts of hydrogen peroxide, namely 13 parts of,
0.03 part of white rot fungus freeze-dried powder.
4. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
the active carbon is coconut shell active carbon, the average particle size is 0.45mm, and the average specific surface area is 3200m2(ii)/g, the pores thereof having an average size of 4 nm;
the white rot fungus jellyThe dry powder was purchased from REUCH Biotechnology Ltd under the brand SHBCC, U.S. A. (NDM3-2), under the Latin namePhanerochaetc chrysosporium。
5. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
the purity of the ethyl acetate is 99.0 percent, and the ethyl acetate is purchased from chemical reagents of national medicine group, Inc.;
the purity of the ferric chloride is 95%, and the ferric chloride is purchased from chemical reagents of national medicine group, Inc.
6. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
the Tris is purchased from Sigma Aldrich trade company Limited and has the purity of 99.9 percent;
the mass concentration of the hydrogen peroxide is 15%.
7. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
controlling the temperature of hydrogen peroxide in the step (1) to be below 8 ℃ when in use;
the soaking temperature in the step (1) is 45 ℃;
the mass of the pure water in the step (1) is three times of that of the activated carbon.
8. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
the culture solution in the step (2) is PDY culture solution;
the temperature of shake flask culture in the step (2) is 37 ℃;
the shake flask culture time in the step (2) is 4 days.
9. The soil remediation agent for chlorophenol-contaminated soil according to claim 1, wherein:
the culture medium for the biofilm attachment culture in the step (2) is an improved PDY culture solution, wherein the addition amount of tryptophan is 15 mg/L.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104229974A (en) * | 2014-09-18 | 2014-12-24 | 浙江理工大学 | Method for rapidly treating organic wastewater |
| CN104313008A (en) * | 2014-10-15 | 2015-01-28 | 湖南大学 | Compound microbial preparation as well as preparation method and application thereof |
| CN105107479A (en) * | 2015-09-06 | 2015-12-02 | 中国石油大学(北京) | Organic modified activated carbon material and preparation method and application thereof |
| CN106047850A (en) * | 2016-05-24 | 2016-10-26 | 浙江清华长三角研究院 | Microbe immobilizing agent as well as preparation method and applications thereof |
| CN106964644A (en) * | 2017-02-24 | 2017-07-21 | 北京绿安创华环保科技股份有限公司 | A kind of organic matter sludges soil-repairing agent, preparation method and its application in sludge is dissolved |
| CN107988096A (en) * | 2017-03-10 | 2018-05-04 | 厦门天邦润生物科技有限公司 | A kind of multifunctional microbial soil-repairing agent and its preparation and application |
| CN110814018A (en) * | 2018-08-09 | 2020-02-21 | 西南大学 | Soil remediation method based on oyster mushroom and phanerochaete chrysosporium |
| CN112774646A (en) * | 2021-01-11 | 2021-05-11 | 陈章平 | Preparation method of activated carbon capable of regenerating and adsorbing methyl orange dye |
| CN113214840A (en) * | 2021-05-12 | 2021-08-06 | 淮阴师范学院 | Preparation method and application of biodegradable carbon particles |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2992469A1 (en) * | 2017-01-23 | 2018-07-23 | Viridis Terra Innovations | Seeding formulation for woody plants and pre-treatment of land surfaces |
-
2021
- 2021-09-06 CN CN202111037328.7A patent/CN113751495B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104229974A (en) * | 2014-09-18 | 2014-12-24 | 浙江理工大学 | Method for rapidly treating organic wastewater |
| CN104313008A (en) * | 2014-10-15 | 2015-01-28 | 湖南大学 | Compound microbial preparation as well as preparation method and application thereof |
| CN105107479A (en) * | 2015-09-06 | 2015-12-02 | 中国石油大学(北京) | Organic modified activated carbon material and preparation method and application thereof |
| CN106047850A (en) * | 2016-05-24 | 2016-10-26 | 浙江清华长三角研究院 | Microbe immobilizing agent as well as preparation method and applications thereof |
| CN106964644A (en) * | 2017-02-24 | 2017-07-21 | 北京绿安创华环保科技股份有限公司 | A kind of organic matter sludges soil-repairing agent, preparation method and its application in sludge is dissolved |
| CN107988096A (en) * | 2017-03-10 | 2018-05-04 | 厦门天邦润生物科技有限公司 | A kind of multifunctional microbial soil-repairing agent and its preparation and application |
| CN110814018A (en) * | 2018-08-09 | 2020-02-21 | 西南大学 | Soil remediation method based on oyster mushroom and phanerochaete chrysosporium |
| CN112774646A (en) * | 2021-01-11 | 2021-05-11 | 陈章平 | Preparation method of activated carbon capable of regenerating and adsorbing methyl orange dye |
| CN113214840A (en) * | 2021-05-12 | 2021-08-06 | 淮阴师范学院 | Preparation method and application of biodegradable carbon particles |
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