CN110093292A - A kind of composite biological agent and preparation method and processing method for handling domestic sludge - Google Patents
A kind of composite biological agent and preparation method and processing method for handling domestic sludge Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The composite biological agent and preparation method and processing method that the invention discloses a kind of for handling domestic sludge, composite biological agent, comprising: alcaligenes, Arthrobacter and germ oligotrophy unit cell category；Wherein, the weight ratio 22-26:15-20:10-14 that alcaligenes, Arthrobacter and germ oligotrophy unit cell belong to.Specific strain is combined together by the present invention, under normal temperature conditions to the organic substance that can be effectively decomposed in sludge after the Treatment of Sludge regular hour, and is made sludge stabilizing by standing certain time, can be effectively removed ammonia, H in sludge2S removes the stench in sludge, while can make most moisture abjections in sludge in a short time, treated, and sludge can be used as soil conditioner, organic fertilizer etc., it is directly used in the plantation of crops, will not influence the growth and development of crops, the quality of crops will not be influenced.
Description
Technical field
The present invention relates to domestic sludge processing technology fields, and in particular to a kind of for handling the compound bio of domestic sludge
Preparation and preparation method and processing method.
Background technique
In recent years, the sewage treatment works in China had unprecedented development, and sewage treatment plant is solving China's water
Play the role of in terms of pollution problem huge, largely alleviates the crisis of China's water pollution.However, sewage treatment
The sludge problem that factory generates also becomes increasingly conspicuous, and has caused the highest attention of various circles of society, Government departments and environmental protection sector
Expert also solves the problems, such as the sludge pollution got worse taking positive countermeasure.
Both at home and abroad common method for sludge treatment there is earth's surface to throw aside, sanitary landfills, ocean dispensing, compost, burning.Earth's surface is abandoned
It sets due to being forbidden by existing environmental regulation just in phased out use.Sanitary landfills is only that municipal sludge has been delayed to generate pollution
Time, harmful substance and strong stench in municipal sludge finally can still enter in the circulation of atmosphere and water resource, cause
Serious environmental pollution endangers human health, while it is more in short supply to also result in land resource.Meeting Marine Pollution is launched in ocean,
It threatens to marine ecosystems and human foods chain.Contain various pathogenic bacteria, worm's ovum, heavy metal etc. in municipal sludge
Harmful substance, and strong stench is distributed, use municipal sludge as farmland fertilizer, serious environmental pollution can be directly contributed,
Even if the method using compost handles municipal sludge, although the danger of the biological substances such as various pathogenic bacteria, worm's ovum can be reduced
Evil, but can not still eliminate the hazards of pollutants such as heavy metal.Municipal sludge is burned, but incendivity object in sludge
Calorific value it is lower, mass energy can be expended using incinerating method processing municipal sludge, and also inevitable in burning process
Harmful substance is generated, secondary pollution is generated to environment.
Therefore, it is urgently problem to be resolved now that municipal sludge, which how is effectively treated,.
Summary of the invention
For above-mentioned deficiency in the prior art, the present invention provides a kind of for handling the compound bio system of domestic sludge
Agent and preparation method and processing method can effectively deal with municipal sludge, eliminate the stench in sludge, reduce a huge sum of money in sludge
Belong to content, and treatment process does not pollute the environment, and will not endanger human health.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is:
It is a kind of for handling the composite biological agent of domestic sludge, comprising: alcaligenes, Arthrobacter and thermophilic malt are few
Support zygosaccharomyces；Wherein, the weight ratio 22-26:15-20 that alcaligenes, Arthrobacter and germ oligotrophy unit cell belong to:
10-14。
Further, the weight ratio that alcaligenes, Arthrobacter and germ oligotrophy unit cell belong to is 25:18:12.
Further, further includes: bacillus, Citrobacter and pseudomonas；Wherein, alcaligenes,
Arthrobacter, germ oligotrophy unit cell category, bacillus, Citrobacter and pseudomonas weight ratio be 22-
26:15-20:10-14:7-9:4-6:1-3。
Further, alcaligenes, Arthrobacter, germ oligotrophy unit cell category, bacillus, citric acid bacillus
Belong to and the weight ratio of pseudomonas is 25:18:12:8:5:2.
Further, the bacterial strain of alcaligenes is Bacillus alcaligenes；The bacterial strain of Arthrobacter is arthrobacterium；Thermophilic malt oligotrophy
The bacterial strain of zygosaccharomyces is Stenotrophomonas maltophilia.
Further, the bacterial strain of bacillus be bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis,
Bacillus, bacillus amyloliquefaciens, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus are pressed
Weight ratio is the Mixed Microbes of 1-3:1-3:1-3:1-3:1-3:1-3:1-3:1-3:1-3 mixing；The bacterial strain of Citrobacter is
Method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, mine-laying kirschner citric acid bacillus, Ke Se Shi citric acid bacillus are in mass ratio
For the Mixed Microbes of 2-5:1-3:1-3:1-3 mixing；The bacterial strain of pseudomonas is pseudomonas aeruginosa, Pseudomonas fluorescens, evil
Smelly pseudomonad and Pseudomonas alcaligenes are the Mixed Microbes of 3-6:2-4:1-3:1-3 mixing in mass ratio.
Further, the bacterial strain of bacillus be bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis,
Bacillus, bacillus amyloliquefaciens, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus are pressed
Weight ratio is the Mixed Microbes of 2:3:3:3:1:1:2:3:2 mixing；The bacterial strain of Citrobacter is method Mo Shi citric acid bacillus, poplar
Ge Shi citric acid bacillus, mine-laying kirschner citric acid bacillus, Ke Se Shi citric acid bacillus are the mixing of 4:2:1:1 mixing in mass ratio
Bacterium；The bacterial strain of pseudomonas is that pseudomonas aeruginosa, Pseudomonas fluorescens, pseudomonas putida and Pseudomonas alcaligenes press matter
Amount is than the Mixed Microbes for 5:3:1:2 mixing.
The preparation method of the above-mentioned composite biological agent for being used to handle domestic sludge, comprising: each category bacterial strain is activated respectively,
Fermentation, obtains fermentation liquid, each fermentation liquid is mixed in proportion, is made；Detailed process are as follows: inclined-plane seed is inoculated into fluid nutrient medium
In activated, then take activation after strain inoculated expanded culture into fermentor, wait expand culture after fermentation liquid
When middle strain is in growth stationary phase, terminates fermentation, then mix in proportion, is made；It gained can also will send out after fermentation
Zymotic fluid carries out freeze-drying and pulvis is made, and is first mixed in water or culture medium handles again sludge when in use；It is compound
Total bacteria count is greater than equal to 5 × 10 in biological agent9A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, then
Composite biological agent is sprayed, primary every 1-2h spray, each spray flow is 1-2L/m3(each spray flow are as follows: every cubic metre of dirt
Mud sprays 1-2L composite biological agent), spray time 5-10min sprays 3-5 days, then stands 5-7 days again.
Further, using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing
Then Chi Zhong sprays composite biological agent, primary every 2h spray, and each spray flow is 1.5L/m3(each spray flow are as follows: every
Cubic meter sludge sprays 1.5L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Provided by the present invention for handling the composite biological agent and preparation method and processing method of domestic sludge, have with
It is lower the utility model has the advantages that
Specific strain is combined together by the present invention, under normal temperature conditions to can be effective after the Treatment of Sludge regular hour
Decompose the organic substance in sludge, and make sludge stabilizing by standing certain time, can effectively remove ammonia in sludge,
H2S removes the stench in sludge, while can make most moisture abjections in sludge in a short time, and treated, and sludge can be made
For soil conditioner, organic fertilizer etc., it is directly used in the plantation of crops, will not influence the growth and development of crops, it will not shadow
Ring the quality of crops.
Alcaligenes can efficiently use ammonia in sludge and nitrate itself to provide influence substance, citric acid bacillus
Category bacterial strain is cooperateed with Bacillus alcaligenes to be played the role of consuming ammonia in sludge, is thereby reduced the ammonia content in sludge, is reduced dirt
The foul odour of mud.
Bacillus can well adapt to this growing environment of sludge, and can decompose the organic substance in sludge, promote
Into the extensive use of chemical fertilizer of sludge.If bacillus pumilus can secrete a large amount of lysozymes, which can dissolve microbial cell, make intracellular
Substance release is other microorganisms such as bacillus licheniformis and Bacillus polymyxa and alcaligenes, Arthrobacter, thermophilic wheat
Bud Stenotrophomonas category, Citrobacter and pseudomonas provide nutriment, it is made to play preferably effect, potent to go
Except stinks and bubbles such as hydrogen sulfide, ammonia nitrogens in sludge, and the organic matter in sludge of degrading, while the weight that can also be complexed in sludge
Metal reduces the content of Heavy Metals in Sludge.
Bacterial strain in the selected pseudomonas of the present invention is organic chemoheterotrophic bacteria, using organic carbon compound as carbon source,
Under the conditions of supporting well, the phosphorus in sludge can be effectively absorbed.
Arthrobacter and germ oligotrophy unit cell category are combined together, and can be cooperateed with and be played the role of desulfurization, can effectively go
Except the H in sludge2S further reduces the foul smell of sludge generation.
Specific embodiment
Embodiment 1
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium and thermophilic malt oligotrophy unit cell
Bacterium composition；Wherein, the weight ratio 22:15:10 of Bacillus alcaligenes, arthrobacterium and germ oligotrophy unit cell.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: by arthrobacterium and thermophilic malt oligotrophy
Monad is inoculated into LB liquid medium is activated respectively, then takes activated bacterium solution to be inoculated into fermentor and is expanded
Big culture terminates fermentation, then mixes fermentation liquid according to aforementioned proportion when the bacterial strain after expanding culture is in growth stationary phase
It closes, total bacteria count is greater than 5 × 10 in mixed fermentation liquid9A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 1h spray, each spray flow is 1L/m3(every cubic metre
Sludge sprays 1L composite biological agent), spray time 5min sprays 3 days, then stands 7 days again.
Embodiment 2
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium and thermophilic malt oligotrophy unit cell
Bacterium composition；Wherein, the weight ratio 26:20:14 of Bacillus alcaligenes, arthrobacterium and germ oligotrophy unit cell.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: by arthrobacterium and thermophilic malt oligotrophy
Monad is inoculated into LB liquid medium is activated respectively, then takes activated bacterium solution to be inoculated into fermentor and is expanded
Big culture terminates fermentation, then mixes fermentation liquid according to aforementioned proportion when the bacterial strain after expanding culture is in growth stationary phase
It closes, total bacteria count is greater than 5 × 10 in mixed fermentation liquid9A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 1h spray, each spray flow is 1L/m3(every cubic metre
Sludge sprays 1L composite biological agent), spray time 5min sprays 3 days, then stands 7 days again.
Embodiment 3
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium and thermophilic malt oligotrophy unit cell
Bacterium composition；Wherein, the weight ratio 25:18:12 of Bacillus alcaligenes, arthrobacterium and germ oligotrophy unit cell.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: by arthrobacterium and thermophilic malt oligotrophy
Monad is inoculated into LB liquid medium is activated respectively, then takes activated bacterium solution to be inoculated into fermentor and is expanded
Big culture terminates fermentation, then mixes fermentation liquid according to aforementioned proportion when the bacterial strain after expanding culture is in growth stationary phase
It closes, total bacteria count is greater than 5 × 10 in mixed fermentation liquid9A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 1h spray, each spray flow is 1L/m3(every cubic metre
Sludge sprays 1L composite biological agent), spray time 5min sprays 3 days, then stands 7 days again.
Embodiment 4
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy unit cell
Bacterium and bacillus (bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, solution starch bud
Spore bacillus, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus by etc. weight mixing mixing
Bacterium), Citrobacter (method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, mine-laying kirschner citric acid bacillus, Ke Se Shi
Citric acid bacillus is the Mixed Microbes of 2:1:1:1 mixing in mass ratio) and pseudomonas (pseudomonas aeruginosa, Pseudomonas
Bacterium, pseudomonas putida and Pseudomonas alcaligenes are the Mixed Microbes of 3:2:1:1 mixing in mass ratio) composition；Wherein, alkali bar is produced
Bacterium, arthrobacterium, germ oligotrophy unit cell, bacillus, Citrobacter and pseudomonas weight ratio be 22:
15:10:7:4:1。
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: that each bacterial strain is inoculated into LB respectively
It is activated in fluid nutrient medium, then takes activated bacterium solution to be inoculated into fermentor and expand culture, wait expand culture
When bacterial strain afterwards is in growth stationary phase, terminates fermentation, then mix fermentation liquid according to aforementioned proportion, mixed fermentation liquid
Middle total bacteria count is greater than 5 × 109A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 2h spray, each spray flow is 2L/m3(every cubic metre
Sludge sprays 2L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Embodiment 5
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy unit cell
Bacterium and bacillus (bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, solution starch bud
Spore bacillus, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus are 3:2:1:3 by weight:
2:1:3:3:1 mixing Mixed Microbes), Citrobacter (method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, cloth rake
Family name's citric acid bacillus, Ke Se Shi citric acid bacillus are the Mixed Microbes of 5:3:3:3 mixing in mass ratio) and pseudomonas (verdigris
Pseudomonad, Pseudomonas fluorescens, pseudomonas putida and Pseudomonas alcaligenes are the mixing of 6:4:3:3 mixing in mass ratio
Bacterium) composition；Wherein, Bacillus alcaligenes, arthrobacterium, germ oligotrophy unit cell, bacillus, Citrobacter and false unit cell
The weight ratio of Pseudomonas is 26:20:14:9:6:3.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: that each bacterial strain is inoculated into LB respectively
It is activated in fluid nutrient medium, then takes activated bacterium solution to be inoculated into fermentor and expand culture, wait expand culture
When bacterial strain afterwards is in growth stationary phase, terminates fermentation, then mix fermentation liquid according to aforementioned proportion, mixed fermentation liquid
Middle total bacteria count is greater than 5 × 109A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 2h spray, each spray flow is 1L/m3(every cubic metre
Sludge sprays 1L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Embodiment 6
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy unit cell
Bacterium and bacillus (bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, solution starch bud
Spore bacillus, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus are 2:3:3:3 by weight:
1:1:2:3:2 mixing Mixed Microbes), Citrobacter (method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, cloth rake
Family name's citric acid bacillus, Ke Se Shi citric acid bacillus are the Mixed Microbes of 4:2:1:1 mixing in mass ratio) and pseudomonas (verdigris
Pseudomonad, Pseudomonas fluorescens, pseudomonas putida and Pseudomonas alcaligenes are the mixing of 5:3:1:2 mixing in mass ratio
Bacterium) composition；Wherein, Bacillus alcaligenes, arthrobacterium, germ oligotrophy unit cell, bacillus, Citrobacter and false unit cell
The weight ratio of Pseudomonas is 25:18:12:8:5:2.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: that each bacterial strain is inoculated into LB respectively
It is activated in fluid nutrient medium, then takes activated bacterium solution to be inoculated into fermentor and expand culture, wait expand culture
When bacterial strain afterwards is in growth stationary phase, terminates fermentation, then mix fermentation liquid according to aforementioned proportion, mixed fermentation liquid
Middle total bacteria count is greater than 5 × 109A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 2h spray, each spray flow is 1.5L/m3(every cube
Rice sludge sprays 1.5L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Embodiment 7
It is a kind of for handling the composite biological agent of domestic sludge, including Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy unit cell
Bacterium and bacillus (bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, solution starch bud
Spore bacillus, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus are 2:3:3:3 by weight:
1:1:2:3:2 mixing Mixed Microbes), Citrobacter (method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, cloth rake
Family name's citric acid bacillus, Ke Se Shi citric acid bacillus are the Mixed Microbes of 4:2:1:1 mixing in mass ratio) and pseudomonas (verdigris
Pseudomonad, Pseudomonas fluorescens, pseudomonas putida and Pseudomonas alcaligenes are the mixing of 5:3:1:2 mixing in mass ratio
Bacterium)；Wherein, Bacillus alcaligenes, arthrobacterium, germ oligotrophy unit cell, bacillus, Citrobacter and pseudomonas
Weight ratio be 25:18:12:8:5:2；The composite biological agent further include halococci, basophilic coccus, Nitrospira gracilis and
Anthropi, halococci, basophilic coccus, Nitrospira gracilis, anthropi and Bacillus alcaligenes weight ratio be 5:2:3:2:
25。
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: that each bacterial strain is inoculated into LB respectively
It is activated in fluid nutrient medium, then takes activated bacterium solution to be inoculated into fermentor and expand culture, wait expand culture
When bacterial strain afterwards is in growth stationary phase, terminates fermentation, then mix fermentation liquid according to aforementioned proportion, mixed fermentation liquid
Middle total bacteria count is greater than 5 × 109A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 2h spray, each spray flow is 1.5L/m3(every cube
Rice sludge sprays 1.5L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Comparative example 1
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy unit cell
Bacterium and bacillus (bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, solution starch bud
Spore bacillus, bacillus mycoides, bacillus licheniformis, bacillus subtilis, bacillus cereus are 2:3:3:3 by weight:
The Mixed Microbes of 1:1:2:3:2 mixing) and pseudomonas (pseudomonas aeruginosa, Pseudomonas fluorescens, pseudomonas putida and production
Pseudomonas alcaligenes are the Mixed Microbes of 5:3:1:2 mixing in mass ratio) composition；Wherein, Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy
Monad, bacillus, Citrobacter and the weight ratio of pseudomonas are 25:18:12:8:2.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: that each bacterial strain is inoculated into LB respectively
It is activated in fluid nutrient medium, then takes activated bacterium solution to be inoculated into fermentor and expand culture, wait expand culture
When bacterial strain afterwards is in growth stationary phase, terminates fermentation, then mix fermentation liquid according to aforementioned proportion, mixed fermentation liquid
Middle total bacteria count is greater than 5 × 109A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 2h spray, each spray flow is 1.5L/m3(every cube
Rice sludge sprays 1.5L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Comparative example 2
It is a kind of for handling the composite biological agent of domestic sludge, by Bacillus alcaligenes, arthrobacterium, thermophilic malt oligotrophy unit cell
Bacterium and bacillus (Sphingobacterium mizutae, acetobacter, non-bacillus, bacillus firmus, thermophilic salt bacillus, gill fungus
Shape bacillus, bacillus licheniformis, bacillus subtilis, bacillus cereus are 2:3:3:3:1:1:2:3:2 by weight
Mixed Mixed Microbes), Citrobacter (method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, mine-laying kirschner citric acid bar
Bacterium, Ke Se Shi citric acid bacillus are the Mixed Microbes of 4:2:1:1 mixing in mass ratio) and pseudomonas (rhodopseudomonas acidophilus,
Rhodopseudomonas palustris, pseudomonas putida and Pseudomonas alcaligenes are the Mixed Microbes of 5:3:1:2 mixing in mass ratio) composition；
Wherein, Bacillus alcaligenes, arthrobacterium, germ oligotrophy unit cell, bacillus, Citrobacter and the weight of pseudomonas
Amount is than being 25:18:12:8:5:2.
The preparation method of the above-mentioned composite biological agent for handling domestic sludge includes: that each bacterial strain is inoculated into LB respectively
It is activated in fluid nutrient medium, then takes activated bacterium solution to be inoculated into fermentor and expand culture, wait expand culture
When bacterial strain afterwards is in growth stationary phase, terminates fermentation, then mix fermentation liquid according to aforementioned proportion, mixed fermentation liquid
Middle total bacteria count is greater than 5 × 109A/mL.
Using the method for above-mentioned composite biological agent processing domestic sludge, comprising: sludge is layed in processing pond, is laid with
With a thickness of 15-20cm, composite biological agent is then sprayed, primary every 2h spray, each spray flow is 1.5L/m3(every cube
Rice sludge sprays 1.5L composite biological agent), spray time 10min sprays 5 days, then stands 7 days again.
Using the embodiment 1-7 and comparative example 1-2 composite biological agent provided and processing method to municipal sludge
It is handled, each embodiment and comparative example is repeated 3 times, according to " agricultural sludge pollutant catabolic gene standard " (GB 4284-2018)
Come to treated, sludge is detected, testing result is the average value of 3 processing, and detection project and testing result are seen below
Table:
Table 1 is life Heavy Metals in Sludge detection project and standard limited value
Content of beary metal variation before and after 2 Treatment of Sludge of table
Other than detecting to Heavy Metal Level In Sludge, the variation of organic pollution materials, detection knot are also had detected
Fruit see the table below:
Organic pollution materials detection project and standard limited value in 3 domestic sludge of table
Organic pollution materials changes of contents before and after 4 Treatment of Sludge of table
In addition to above-mentioned detection project, the application also has detected moisture content and H before and after Treatment of Sludge2S content.At sludge
Moisture content is 82% before managing, and after the processing of embodiment 6, moisture content is down to 12%, and embodiment 7, comparative example 1 and comparative example
After 2 processing, moisture content is respectively 25%, 22% and 25%；H before Treatment of Sludge2S content is 68mg/m3, handled through embodiment 6
Afterwards, H2S content is down to 0.012mg/m3, and after embodiment 7, comparative example 1 and comparative example 2 are handled, H2S content is respectively
0.212mg/m3、0.579mg/m3And 0.456mg/m3。
By above-mentioned testing result it is found that the composite biological agent provided through the invention at normal temperature carries out domestic sludge
Processing, can reduce Heavy Metal Level In Sludge, organic pollutant content, can accelerate sludge dewatering, composite biological agent of the present invention
And processing method is substantially better than comparative example 1 and comparative example 2, it is processed by the invention after domestic sludge indices reach agricultural
Sludge A grade standard.In addition, H in sludge2S content reduces very much, and close to olfact, ammonia content is as the loss of moisture also subtracts
Lack very much, therefore, the basic no foul smell of sludge that treated.And comparative example 1 and comparative example 2 can also smell ratio within 10m
Thin foul smell.
By embodiment 6, embodiment 7, comparative example 1 and comparative example 2, treated that sludge is used to plant crops tomato, Huang
Melon, eggplant, cauliflower and grape are planted using greenhouse gardening mode, and control group is normal soil, and in experimental group per acre
Be added 500 jin processing after sludge, planting process is consistent with control group, and per mu yield see the table below:
The influence of 5 experimental group of table and control group to crop yield
| Embodiment 6 | Embodiment 7 | Comparative example 1 | Comparative example 2 | Control group | |
| Tomato (kg/ mus) | 6500 | 6200 | 4800 | 5000 | 6800 |
| Cucumber (kg/ mus) | 12500 | 10000 | 8000 | 8500 | 12000 |
| Eggplant (kg/ mus) | 2900 | 2400 | 2000 | 2000 | 3000 |
| Cauliflower (kg/ mus) | 4300 | 3800 | 3500 | 3700 | 4200 |
| Grape (kg/ mus) | 2300 | 1500 | 1800 | 1920 | 2800 |
As seen from the above table, treated that sludge can be used for planting crops by the present invention, little with control group difference, and compares
Example 1 and comparative example 2 and control group gap are larger.And embodiment 6, embodiment 7, comparative example 1, comparative example 2 and control group are given birth to
Tomato, cucumber, eggplant, cauliflower and the grape of production compare, and the crops of all groups of productions are uniform in size, pure color,
Mouthfeel is good.Thus the composite biological agent provided through the invention is provided treated that sludge application value is very high, thus to useless
Gurry is recycled, and has good economic value.
Claims (10)
1. a kind of for handling the composite biological agent of domestic sludge characterized by comprising alcaligenes, Arthrobacter
With germ oligotrophy unit cell category；Wherein, the weight ratio 22- that alcaligenes, Arthrobacter and germ oligotrophy unit cell belong to
26:15-20:10-14。
2. according to claim 1 for handling the composite biological agent of domestic sludge, which is characterized in that Bacillus alcaligenes
The weight ratio that category, Arthrobacter and germ oligotrophy unit cell belong to is 25:18:12.
3. according to claim 1 or 2 for handling the composite biological agent of domestic sludge, which is characterized in that further include:
Bacillus, Citrobacter and pseudomonas；Wherein, alcaligenes, Arthrobacter, germ oligotrophy unit cell
Category, bacillus, Citrobacter and the weight ratio of pseudomonas are 22-26:15-20:10-14:7-9:4-6:1-
3。
4. according to claim 3 for handling the composite biological agent of domestic sludge, which is characterized in that Bacillus alcaligenes
Category, Arthrobacter, germ oligotrophy unit cell category, bacillus, Citrobacter and the weight ratio of pseudomonas are
25:18:12:8:5:2。
5. according to claim 1 for handling the composite biological agent of domestic sludge, which is characterized in that alcaligenes
Bacterial strain be Bacillus alcaligenes；The bacterial strain of Arthrobacter is arthrobacterium；The bacterial strain that germ oligotrophy unit cell belongs to is thermophilic malt oligotrophy
Sporangium.
6. according to claim 3 for handling the composite biological agent of domestic sludge, which is characterized in that bacillus
Bacterial strain be bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, bacillus amyloliquefaciens, gill fungus shape
Bacillus, bacillus licheniformis, bacillus subtilis, bacillus cereus are 1-3:1-3:1-3:1-3:1-3 by weight:
The Mixed Microbes of 1-3:1-3:1-3:1-3 mixing；The bacterial strain of Citrobacter is method Mo Shi citric acid bacillus, poplar Ge Shi citric acid
Bacillus, mine-laying kirschner citric acid bacillus, Ke Se Shi citric acid bacillus are the mixing of 2-5:1-3:1-3:1-3 mixing in mass ratio
Bacterium；The bacterial strain of pseudomonas is that pseudomonas aeruginosa, Pseudomonas fluorescens, pseudomonas putida and Pseudomonas alcaligenes press matter
Amount is than the Mixed Microbes for 3-6:2-4:1-3:1-3 mixing.
7. according to claim 6 for handling the composite biological agent of domestic sludge, which is characterized in that bacillus
Bacterial strain be bacillus pumilus, Bacillus polymyxa, bacillus thuringiensis, bacillus, bacillus amyloliquefaciens, gill fungus shape
Bacillus, bacillus licheniformis, bacillus subtilis, bacillus cereus mix by weight for 2:3:3:3:1:1:2:3:2
The Mixed Microbes of conjunction；The bacterial strain of Citrobacter is method Mo Shi citric acid bacillus, poplar Ge Shi citric acid bacillus, mine-laying kirschner lemon
Acidfast bacilli, Ke Se Shi citric acid bacillus are the Mixed Microbes of 4:2:1:1 mixing in mass ratio；The bacterial strain of pseudomonas is that verdigris is false
Monad, Pseudomonas fluorescens, pseudomonas putida and Pseudomonas alcaligenes are the Mixed Microbes of 5:3:1:2 mixing in mass ratio.
8. claim 1-7 is described in any item for handling the preparation method of the composite biological agent of domestic sludge, feature
It is, comprising: each category bacterial strain is activated respectively, is fermented, is obtained fermentation liquid, each fermentation liquid is mixed in proportion, in mixed fermentation liquid
Total bacteria count is more than or equal to 5 × 109A/mL is made.
9. according to claim 8 for handling the preparation method of the composite biological agent of domestic sludge, which is characterized in that
Further include being freeze-dried mixed fermentation liquid, pulvis is made.
10. using the method for the described in any item composite biological agent processing domestic sludges of claim 1-7, comprising: by sludge
It is layed in processing pond, then sprays composite biological agent, primary every 1-2h spray, each spray flow is 1-2L/m3, spray
Time is 5-10min, sprays 3-5 days, then stands 5-7 days again；Wherein, when composite biological agent is pulvis, pulvis is mixed
In Yu Shui or fluid nutrient medium, so that total bacteria count is more than or equal to 5 × 10 in liquid9A/mL.
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Application publication date: 20190806 |