CN107032574A - Municipal sludge processing composite bacteria agent and preparation method thereof - Google Patents
Municipal sludge processing composite bacteria agent and preparation method thereof Download PDFInfo
- Publication number
- CN107032574A CN107032574A CN201710432800.4A CN201710432800A CN107032574A CN 107032574 A CN107032574 A CN 107032574A CN 201710432800 A CN201710432800 A CN 201710432800A CN 107032574 A CN107032574 A CN 107032574A
- Authority
- CN
- China
- Prior art keywords
- parts
- liquid spawn
- liquid
- spawn
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of municipal sludge processing composite bacteria agent and preparation method thereof.The composite bacteria agent includes the component of following parts by weight：10~30 parts of fluorescent pseudomonas liquid spawn；10~30 parts of bacillus subtilis liquid spawn；3~15 parts of Dipel liquid strain；3~15 parts of pseudomonas putida liquid spawn；10~30 parts of Bacillus circulans liquid spawn；3~10 parts of distillery yeast liquid spawn；Breathe out time 3~15 parts of trichoderma liquid spawn；5~15 parts of pseudomonas aeruginosa liquid spawn；8~12 parts of soyabean protein powder；1~3 part of beef extract；1~3 part of agar；8~12 parts of dipotassium hydrogen phosphate；8~12 parts of urea；15~25 parts of molasses.In the present invention strain by individually cultivate it is mature and stable after combine in proportion, it is to avoid strain easily produces antagonism and the problem of contending with is produced between dead and bacterium, can Adsorption of Heavy Metals, promote organic matter conversion, there is good effect to Treatment of Sludge.
Description
Technical field
The present invention relates to biological product technical field, more particularly to a kind of municipal sludge processing composite bacteria agent and its preparation
Method.
Background technology
With the continuous expansion of urbanization, city dweller is more and more, the yield of municipal sludge also more and more higher, ring
Packing pressure also increasingly increases.The processing of current municipal sludge, domestic sludge and river sludge, generally to fill or pour into sea
Handled etc. method, it is a small number of to be handled with the method burned.
At present, although some enterprises are started after being handled using microbial process sludge as Fertilizer application, still
Due to the function of existing biological compound fungi agent is not powerful enough and strain between easily produce antagonism and cause part strain dead
Etc. the influence of factor, Treatment of Sludge effect is not obvious.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of strain Various Functions, perfect collocation and are consistent, can adsorb
Heavy metal, the municipal sludge processing composite bacteria agent for promoting organic matter conversion, making waste resource turn waste into wealth.
In order to solve the above technical problems, the technical scheme is that：Municipal sludge processing composite bacteria agent, including it is as follows
The component of parts by weight：10~30 parts of fluorescent pseudomonas liquid spawn；10~30 parts of bacillus subtilis liquid spawn；Su Yunjin
3~15 parts of bacillus liquid spawn；3~15 parts of pseudomonas putida liquid spawn；10~30 parts of Bacillus circulans liquid spawn；
3~10 parts of distillery yeast liquid spawn；Breathe out time 3~15 parts of trichoderma liquid spawn；5~15 parts of pseudomonas aeruginosa liquid spawn；
8~12 parts of soyabean protein powder；1~3 part of beef extract；1~3 part of agar；8~12 parts of dipotassium hydrogen phosphate；8~12 parts of urea；Molasses
15~25 parts.
Preferably, the municipal sludge processing includes the component of following parts by weight with composite bacteria agent：Fluorescent pseudomonas
20 parts of liquid spawn；20 parts of bacillus subtilis liquid spawn；5 parts of Dipel liquid strain；Pseudomonas putida bacteria liquid
10 parts of strain；20 parts of Bacillus circulans liquid spawn；5 parts of distillery yeast liquid spawn；Breathe out time 5 parts of trichoderma liquid spawn；Copper
Green 10 parts of pseudomonad liquid spawn；10 parts of soyabean protein powder；2 parts of beef extract；2 parts of agar；10 parts of dipotassium hydrogen phosphate；Urea 10
Part；20 parts of molasses.
By adopting the above-described technical solution, the processing of this municipal sludge is had the advantages that with composite bacteria agent：
(1) after the moisture carbon-nitrogen ratio for preparing material, the function for every kind of microorganism is combined as the thalline of advantage, strain work(
Can be different, perfection collocation is consistent；Bacillus wherein comprising the stronger Pseudomonas of fixed nitrogen, flocculation ability and phosphate solubilizing bacteria,
Decompose organic matter, fiber, albumen, stalk and the residual Kazakhstan used time trichoderma of agriculture；Bacillus subtilis, Dipel are to squama wing
Mesh, Diptera and Hymenoptera have stronger lethality, and Bacillus circulans phosphorus decomposing, antidote are typical aerobic organism During High-Temperature Compostings
Bacterium；Pseudomonasputida, which kills line to flocculation compost, good effect, and yeast has good effect to adsorbing metal, and verdigris is false single
Born of the same parents' bacterium energy growing nitrogen-fixing, degrading organic phosphor, compost；This product has powerful survival ability, can quickly breed, with fixed nitrogen,
Soluble phosphorus, the effect for preventing nutriment in a fertilizer to be lost in, have preferable processing to act on for sludge.
(2) strain by individually cultivate it is mature and stable after by a certain percentage combine, it is to avoid multiple-microorganism is cultivated together
Produce antagonism and cause the dead phenomenon of part strain, contending between bacterium is avoided according to the ratio collocation of eubacteria.This product
It is a kind of anti-flora of multifunctional and composite type, the nutrition in sludge can be promoted to change, have good biocontrol effect again, prevent nutriment in a fertilizer
It is lost in, preserves the ecological environment.
, can be according to the property of every kind of material come strain of arranging in pairs or groups, or for the composition of every kind of sludge during concrete application
Analyzed, grasp its demand and need which type of strain, then suitably allocated.
Present invention also offers the preparation method of above-mentioned municipal sludge processing composite bacteria agent, comprise the following steps：
Step 1: the preparation of culture medium
(1) preparation of culture medium A
Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea, beef extract and water that weight content is made for amino acid
1.5~2.5%, potassium dihydrogen phosphate 1.5~2.5%, glucose 0.5~1.5%, peptone 0.1~0.9%, urea 0.1~
0.9%th, the mixed liquor of beef extract 0.5~1.5%, pH value 6.5~7.5 obtains culture medium A；
(2) culture medium B preparation
Take potassium nitrate, sodium chloride, molasses, glucose and water that weight content is made for potassium nitrate 0.3~0.8%, sodium chloride
0.1~0.3%, molasses 3~8%, the mixed liquor of glucose 1~3%, pH value 6.5~7.5 obtains culture medium B；
Step 2: the culture of liquid spawn
Take fluorescent pseudomonas, bacillus subtilis, Dipel, pseudomonas putida, Bacillus circulans, alcohol
Then yeast and pseudomonas aeruginosa are cultivated with 500~1000 grams of culture medium As, are obtained first in desinfection chamber the purification and rejuvenation respectively
To fluorescent pseudomonas liquid spawn, bacillus subtilis liquid spawn, Dipel liquid strain, Pseudomonas putida bacterium solution
Body strain, Bacillus circulans liquid spawn, distillery yeast liquid spawn and pseudomonas aeruginosa liquid spawn；Condition of culture is
28~32 DEG C of shaking table cultures 24~72 hours；
Take Kazakhstan time trichoderma first in desinfection chamber the purification and rejuvenation, then cultivated, breathed out with 500~1000 grams of culture medium B
Secondary trichoderma liquid spawn；Condition of culture is 28~32 DEG C of shaking table cultures 24~72 hours；
Step 3: the curing of liquid spawn
By the fluorescent pseudomonas liquid spawn after being cultivated in step 2, bacillus subtilis liquid spawn, the golden bars of Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn and Kazakhstan time trichoderma liquid spawn put into 90~100 kilograms of sterilising mediums respectively, 25 in seeding tank
~35 DEG C are cultivated 5~10 days, standby after curing；
Step 4: the compound and reinforcing of liquid spawn
Take the golden bar of the fluorescent pseudomonas liquid spawn after being cured in step 3, bacillus subtilis liquid spawn, Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn, Kazakhstan time trichoderma liquid spawn and soyabean protein powder, beef extract, agar, dipotassium hydrogen phosphate, urea, sugar
Honey is mixed into complex in proportion, that is, the municipal sludge processing composite bacteria agent is made.
Preferably, the weight of each component is as follows in step 4：10~30 parts of fluorescent pseudomonas liquid spawn；
10~30 parts of bacillus subtilis liquid spawn；3~15 parts of Dipel liquid strain；Pseudomonas putida liquid spawn 3
~15 parts；10~30 parts of Bacillus circulans liquid spawn；3~10 parts of distillery yeast liquid spawn；Breathe out time trichoderma liquid spawn 3
~15 parts；5~15 parts of pseudomonas aeruginosa liquid spawn；8~12 parts of soyabean protein powder；1~3 part of beef extract；1~3 part of agar；
8~12 parts of dipotassium hydrogen phosphate；8~12 parts of urea；15~25 parts of molasses.
By adopting the above-described technical solution, this preparation method has the advantages that：Utilize the spy of respective microorganism
Property in incubation carry out strain independent culture, be combined after strain is mature and stable and make it into stable state, prevented
Cause some strains on the decline after mixing, it is to avoid multiple-microorganism, which is together cultivated, to be produced antagonism and cause part strain is dead to show
As.This preparation method is simply easily achieved, and this mode is composited strain Various Functions, and perfection collocation is consistent, and can adsorb
Heavy metal, promotes organic matter conversion, accelerates organic matter decomposition, have preferable processing to act on for sludge.
Embodiment
With reference to embodiment, the present invention is expanded on further.
Embodiment 1
In the present embodiment, municipal sludge processing includes the component of following parts by weight with composite bacteria agent：Fluorescent pseudomonas liquid
10 parts of body strain；10 parts of bacillus subtilis liquid spawn；3 parts of Dipel liquid strain；Pseudomonas putida liquid bacteria
Plant 3 parts；10 parts of Bacillus circulans liquid spawn；3 parts of distillery yeast liquid spawn；Breathe out time 3 parts of trichoderma liquid spawn；Verdigris is false
5 parts of monad liquid spawn；8 parts of soyabean protein powder；1 part of beef extract；1 part of agar；8 parts of dipotassium hydrogen phosphate；8 parts of urea；Molasses
15 parts.
Its preparation method comprises the following steps：
Step 1: the preparation of culture medium
(1) preparation of culture medium A
Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea, beef extract and water that weight content is made for amino acid
1.5%th, potassium dihydrogen phosphate 1.5%, glucose 0.5%, peptone 0.2%, urea 0.3%, the mixed liquor of beef extract 0.5%,
PH value 6.5~7.5, obtains culture medium A；
(2) culture medium B preparation
Take potassium nitrate, sodium chloride, molasses, glucose and water that weight content is made for potassium nitrate 0.3%, sodium chloride 0.1%,
Molasses 4%, the mixed liquor of glucose 1%, pH value 6.5~7.5 obtains culture medium B；
Step 2: the culture of liquid spawn
Take fluorescent pseudomonas, bacillus subtilis, Dipel, pseudomonas putida, Bacillus circulans, alcohol
Then yeast and pseudomonas aeruginosa are cultivated with 500~1000 grams of culture medium As, are obtained first in desinfection chamber the purification and rejuvenation respectively
To fluorescent pseudomonas liquid spawn, bacillus subtilis liquid spawn, Dipel liquid strain, Pseudomonas putida bacterium solution
Body strain, Bacillus circulans liquid spawn, distillery yeast liquid spawn and pseudomonas aeruginosa liquid spawn；Condition of culture is
28~32 DEG C of shaking table cultures 24~72 hours；
Take Kazakhstan time trichoderma first in desinfection chamber the purification and rejuvenation, then cultivated, breathed out with 500~1000 grams of culture medium B
Secondary trichoderma liquid spawn；Condition of culture is 28~32 DEG C of shaking table cultures 24~72 hours；
Step 3: the curing of liquid spawn
By the fluorescent pseudomonas liquid spawn after being cultivated in step 2, bacillus subtilis liquid spawn, the golden bars of Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn and Kazakhstan time trichoderma liquid spawn put into 90~100 kilograms of sterilising mediums respectively, 30 in seeding tank
DEG C culture 5~10 days, it is standby after curing；
Step 4: the compound and reinforcing of liquid spawn
Take the golden bar of the fluorescent pseudomonas liquid spawn after being cured in step 3, bacillus subtilis liquid spawn, Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn, Kazakhstan time trichoderma liquid spawn and soyabean protein powder, beef extract, agar, dipotassium hydrogen phosphate, urea, sugar
Honey is mixed into complex by above-mentioned weight ratio, that is, the municipal sludge processing composite bacteria agent is made.
Embodiment 2
In the present embodiment, municipal sludge processing includes the component of following parts by weight with composite bacteria agent：Fluorescent pseudomonas liquid
20 parts of body strain；20 parts of bacillus subtilis liquid spawn；5 parts of Dipel liquid strain；Pseudomonas putida liquid bacteria
Plant 10 parts；20 parts of Bacillus circulans liquid spawn；5 parts of distillery yeast liquid spawn；Breathe out time 5 parts of trichoderma liquid spawn；Verdigris
10 parts of pseudomonad liquid spawn；10 parts of soyabean protein powder；2 parts of beef extract；2 parts of agar；10 parts of dipotassium hydrogen phosphate；Urea 10
Part；20 parts of molasses.
Its preparation method comprises the following steps：
Step 1: the preparation of culture medium
(1) preparation of culture medium A
Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea, beef extract and water that weight content is made for amino acid
2%th, potassium dihydrogen phosphate 2%, glucose 1%, peptone 0.5%, urea 0.5%, the mixed liquor of beef extract 1%, pH value 6.5~
7.5, obtain culture medium A；
(2) culture medium B preparation
Take potassium nitrate, sodium chloride, molasses, glucose and water that weight content is made for potassium nitrate 0.5%, sodium chloride 0.2%,
Molasses 5%, the mixed liquor of glucose 2%, pH value 6.5~7.5 obtains culture medium B；
Step 2: the culture of liquid spawn
Take fluorescent pseudomonas, bacillus subtilis, Dipel, pseudomonas putida, Bacillus circulans, alcohol
Then yeast and pseudomonas aeruginosa are cultivated with 500~1000 grams of culture medium As, are obtained first in desinfection chamber the purification and rejuvenation respectively
To fluorescent pseudomonas liquid spawn, bacillus subtilis liquid spawn, Dipel liquid strain, Pseudomonas putida bacterium solution
Body strain, Bacillus circulans liquid spawn, distillery yeast liquid spawn and pseudomonas aeruginosa liquid spawn；Condition of culture is
28~32 DEG C of shaking table cultures 24~72 hours；
Take Kazakhstan time trichoderma first in desinfection chamber the purification and rejuvenation, then cultivated, breathed out with 500~1000 grams of culture medium B
Secondary trichoderma liquid spawn；Condition of culture is 28~32 DEG C of shaking table cultures 24~72 hours；
Step 3: the curing of liquid spawn
By the fluorescent pseudomonas liquid spawn after being cultivated in step 2, bacillus subtilis liquid spawn, the golden bars of Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn and Kazakhstan time trichoderma liquid spawn put into 90~100 kilograms of sterilising mediums respectively, 30 in seeding tank
DEG C culture 5~10 days, it is standby after curing；
Step 4: the compound and reinforcing of liquid spawn
Take the golden bar of the fluorescent pseudomonas liquid spawn after being cured in step 3, bacillus subtilis liquid spawn, Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn, Kazakhstan time trichoderma liquid spawn and soyabean protein powder, beef extract, agar, dipotassium hydrogen phosphate, urea, sugar
Honey is mixed into complex by above-mentioned weight ratio, that is, the municipal sludge processing composite bacteria agent is made.
Embodiment 3
In the present embodiment, municipal sludge processing includes the component of following parts by weight with composite bacteria agent：Fluorescent pseudomonas liquid
30 parts of body strain；30 parts of bacillus subtilis liquid spawn；15 parts of Dipel liquid strain；Pseudomonas putida liquid bacteria
Plant 15 parts；30 parts of Bacillus circulans liquid spawn；10 parts of distillery yeast liquid spawn；Breathe out time 15 parts of trichoderma liquid spawn；Copper
Green 15 parts of pseudomonad liquid spawn；12 parts of soyabean protein powder；3 parts of beef extract；3 parts of agar；12 parts of dipotassium hydrogen phosphate；Urea 12
Part；25 parts of molasses.
Its preparation method comprises the following steps：
Step 1: the preparation of culture medium
(1) preparation of culture medium A
Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea, beef extract and water that weight content is made for amino acid
2.5%th, potassium dihydrogen phosphate 2.5%, glucose 1.5%, peptone 0.8%, urea 0.8%, the mixed liquor of beef extract 1.5%,
PH value 6.5~7.5, obtains culture medium A；
(2) culture medium B preparation
Take potassium nitrate, sodium chloride, molasses, glucose and water that weight content is made for potassium nitrate 0.8%, sodium chloride 0.3%,
Molasses 8%, the mixed liquor of glucose 3%, pH value 6.5~7.5 obtains culture medium B；
Step 2: the culture of liquid spawn
Take fluorescent pseudomonas, bacillus subtilis, Dipel, pseudomonas putida, Bacillus circulans, alcohol
Then yeast and pseudomonas aeruginosa are cultivated with 500~1000 grams of culture medium As, are obtained first in desinfection chamber the purification and rejuvenation respectively
To fluorescent pseudomonas liquid spawn, bacillus subtilis liquid spawn, Dipel liquid strain, Pseudomonas putida bacterium solution
Body strain, Bacillus circulans liquid spawn, distillery yeast liquid spawn and pseudomonas aeruginosa liquid spawn；Condition of culture is
28~32 DEG C of shaking table cultures 24~72 hours；
Take Kazakhstan time trichoderma first in desinfection chamber the purification and rejuvenation, then cultivated, breathed out with 500~1000 grams of culture medium B
Secondary trichoderma liquid spawn；Condition of culture is 28~32 DEG C of shaking table cultures 24~72 hours；
Step 3: the curing of liquid spawn
By the fluorescent pseudomonas liquid spawn after being cultivated in step 2, bacillus subtilis liquid spawn, the golden bars of Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn and Kazakhstan time trichoderma liquid spawn put into 90~100 kilograms of sterilising mediums respectively, 30 in seeding tank
DEG C culture 5~10 days, it is standby after curing；
Step 4: the compound and reinforcing of liquid spawn
Take the golden bar of the fluorescent pseudomonas liquid spawn after being cured in step 3, bacillus subtilis liquid spawn, Su Yun
Bacteria liquid strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, verdigris are false
Monad liquid spawn, Kazakhstan time trichoderma liquid spawn and soyabean protein powder, beef extract, agar, dipotassium hydrogen phosphate, urea, sugar
Honey is mixed into complex by above-mentioned weight ratio, that is, the municipal sludge processing composite bacteria agent is made.
Schematical embodiment of the invention is the foregoing is only, the scope of the present invention is not limited to.It is any
Those skilled in the art, made equivalent variations and modification on the premise of the design of the present invention and principle is not departed from,
The scope of protection of the invention should be belonged to.
Claims (4)
1. municipal sludge processing composite bacteria agent, it is characterised in that include the component of following parts by weight：Fluorescent pseudomonas liquid
10~30 parts of strain；10~30 parts of bacillus subtilis liquid spawn；3~15 parts of Dipel liquid strain；Stench is false single
3~15 parts of born of the same parents' bacteria liquid strain；10~30 parts of Bacillus circulans liquid spawn；3~10 parts of distillery yeast liquid spawn；Breathe out secondary
3~15 parts of trichoderma liquid spawn；5~15 parts of pseudomonas aeruginosa liquid spawn；8~12 parts of soyabean protein powder；Beef extract 1~3
Part；1~3 part of agar；8~12 parts of dipotassium hydrogen phosphate；8~12 parts of urea；15~25 parts of molasses.
2. municipal sludge processing composite bacteria agent as claimed in claim 1, it is characterised in that include the group of following parts by weight
Part：20 parts of fluorescent pseudomonas liquid spawn；20 parts of bacillus subtilis liquid spawn；5 parts of Dipel liquid strain；Dislike
Smelly 10 parts of pseudomonad liquid spawn；20 parts of Bacillus circulans liquid spawn；5 parts of distillery yeast liquid spawn；Breathe out time trichoderma
5 parts of liquid spawn；10 parts of pseudomonas aeruginosa liquid spawn；10 parts of soyabean protein powder；2 parts of beef extract；2 parts of agar；Phosphoric acid hydrogen
10 parts of dipotassium；10 parts of urea；20 parts of molasses.
3. the preparation method of municipal sludge processing composite bacteria agent, it is characterised in that comprise the following steps：
Step 1: the preparation of culture medium
(1) preparation of culture medium A
Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea, beef extract and water that weight content is made for amino acid/11 .5
~2.5%, potassium dihydrogen phosphate 1.5~2.5%, glucose 0.5~1.5%, peptone 0.1~0.9%, urea 0.1~
0.9%th, the mixed liquor of beef extract 0.5~1.5%, pH value 6.5~7.5 obtains culture medium A；
(2) culture medium B preparation
Take potassium nitrate, sodium chloride, molasses, glucose and water be made weight content for potassium nitrate 0.3~0.8%, sodium chloride 0.1~
0.3%, molasses 3~8%, the mixed liquor of glucose 1~3%, pH value 6.5~7.5 obtains culture medium B；
Step 2: the culture of liquid spawn
Take fluorescent pseudomonas, bacillus subtilis, Dipel, pseudomonas putida, Bacillus circulans, distillery yeast
With pseudomonas aeruginosa first in desinfection chamber the purification and rejuvenation, then cultivated respectively with 500~1000 grams of culture medium As, obtain glimmering
Light pseudomonas liquid spawn, bacillus subtilis liquid spawn, Dipel liquid strain, pseudomonas putida liquid bacteria
Kind, Bacillus circulans liquid spawn, distillery yeast liquid spawn and pseudomonas aeruginosa liquid spawn；Condition of culture be 28~
32 DEG C of shaking table cultures 24~72 hours；
Take Kazakhstan time trichoderma first in desinfection chamber the purification and rejuvenation, then cultivated with 500~1000 grams of culture medium B, obtain breathing out time wood
Mould liquid spawn；Condition of culture is 28~32 DEG C of shaking table cultures 24~72 hours；
Step 3: the curing of liquid spawn
By the fluorescent pseudomonas liquid spawn after being cultivated in step 2, bacillus subtilis liquid spawn, thuringiensis bacillus liquid
Body strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, P. aeruginosa
Bacteria liquid strain and Kazakhstan time trichoderma liquid spawn put into 90~100 kilograms of sterilising mediums respectively, 25~35 in seeding tank
DEG C culture 5~10 days, it is standby after curing；
Step 4: the compound and reinforcing of liquid spawn
Take the fluorescent pseudomonas liquid spawn after being cured in step 3, bacillus subtilis liquid spawn, thuringiensis bacillus liquid
Body strain, pseudomonas putida liquid spawn, Bacillus circulans liquid spawn, distillery yeast liquid spawn, P. aeruginosa
Bacteria liquid strain, Kazakhstan time trichoderma liquid spawn and soyabean protein powder, beef extract, agar, dipotassium hydrogen phosphate, urea, molasses are pressed
Ratio is mixed into complex, that is, the municipal sludge processing composite bacteria agent is made.
4. the preparation method of municipal sludge processing composite bacteria agent as claimed in claim 3, it is characterised in that each in step 4
The weight of component is as follows：10~30 parts of fluorescent pseudomonas liquid spawn；Bacillus subtilis liquid spawn 10~30
Part；3~15 parts of Dipel liquid strain；3~15 parts of pseudomonas putida liquid spawn；Bacillus circulans liquid spawn
10~30 parts；3~10 parts of distillery yeast liquid spawn；Breathe out time 3~15 parts of trichoderma liquid spawn；Pseudomonas aeruginosa liquid spawn
5~15 parts；8~12 parts of soyabean protein powder；1~3 part of beef extract；1~3 part of agar；8~12 parts of dipotassium hydrogen phosphate；Urea 8~12
Part；15~25 parts of molasses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710432800.4A CN107032574A (en) | 2017-06-09 | 2017-06-09 | Municipal sludge processing composite bacteria agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710432800.4A CN107032574A (en) | 2017-06-09 | 2017-06-09 | Municipal sludge processing composite bacteria agent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107032574A true CN107032574A (en) | 2017-08-11 |
Family
ID=59541903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710432800.4A Pending CN107032574A (en) | 2017-06-09 | 2017-06-09 | Municipal sludge processing composite bacteria agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107032574A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093292A (en) * | 2019-05-07 | 2019-08-06 | 成都市锦鑫汇生物科技有限公司 | A kind of composite biological agent and preparation method and processing method for handling domestic sludge |
| CN117486575A (en) * | 2024-01-03 | 2024-02-02 | 中建安装集团有限公司 | Low-carbon solidified material with high organic matter residue soil content and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234615A (en) * | 2010-04-22 | 2011-11-09 | 江苏同人环保科技有限公司 | High-efficiency combined functional bacteria for sludge treatment |
| CN103205384A (en) * | 2013-04-17 | 2013-07-17 | 广州利万世环保科技有限公司 | Soil heavy metal hexavalent chromium biological degrading agent and production method thereof |
| CZ304378B6 (en) * | 2013-03-28 | 2014-04-02 | Dekonta A.S. | Biodegradable maintenance method of contaminated soil |
-
2017
- 2017-06-09 CN CN201710432800.4A patent/CN107032574A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234615A (en) * | 2010-04-22 | 2011-11-09 | 江苏同人环保科技有限公司 | High-efficiency combined functional bacteria for sludge treatment |
| CZ304378B6 (en) * | 2013-03-28 | 2014-04-02 | Dekonta A.S. | Biodegradable maintenance method of contaminated soil |
| CN103205384A (en) * | 2013-04-17 | 2013-07-17 | 广州利万世环保科技有限公司 | Soil heavy metal hexavalent chromium biological degrading agent and production method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 夏焕章主编: "《发酵工艺学》", 31 July 2007 * |
| 沈萍等: "《微生物学》", 30 August 2009, 高等教育出版社 * |
| 潘勇等: "《水产品质量检验员》", 30 September 2016 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093292A (en) * | 2019-05-07 | 2019-08-06 | 成都市锦鑫汇生物科技有限公司 | A kind of composite biological agent and preparation method and processing method for handling domestic sludge |
| CN117486575A (en) * | 2024-01-03 | 2024-02-02 | 中建安装集团有限公司 | Low-carbon solidified material with high organic matter residue soil content and preparation method and application thereof |
| CN117486575B (en) * | 2024-01-03 | 2024-05-07 | 中建安装集团有限公司 | Low-carbon solidified material with high organic matter residue soil content and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110498712B (en) | Biological organic fertilizer and preparation method thereof | |
| CN101899397B (en) | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application | |
| CN108441444A (en) | A kind of complex micro organism fungicide administered suitable for black and odorous water | |
| CN101676225B (en) | A new microbe agent and preparation method thereof | |
| CN100374547C (en) | Composite bacillus subtilis and lactic acid bacteria microbe formulation preparation method | |
| CN103205382A (en) | Microbial agent for purifying river wastewater and preparation method of microbial agent | |
| CN102344315A (en) | Composite microbial seaweed organic liquid fertilizer and preparation method thereof | |
| CN105384480A (en) | Biological humic acid organic material decomposition agent having deodorization function | |
| CN104449744A (en) | Microbial soil remediation agent and preparation method thereof | |
| CN107164270A (en) | Prevent and treat soil-borne disease composite bacteria agent and preparation method thereof | |
| CN101255402A (en) | Thallus for producing biological fertilizer by employing cane sugar filter mud fermentation | |
| CN104649408A (en) | Bio-enzyme preparation for aerobic section process for municipal and industrial wastewater treatment | |
| CN102093975A (en) | Composite fungicide for rapid degradation of organic waste and applications thereof | |
| CN102776138A (en) | Compound microorganism preparation for materialized sludge treatment and method for preparing same | |
| CN106701628B (en) | Rural domestic garbage fermentation microbial inoculum and use method and application thereof | |
| CN102864109B (en) | Preparation for trinitrotoluene-contained sewage treatment and using method of preparation | |
| CN106587559A (en) | Sludge anaerobic digestion method | |
| CN107879774A (en) | A kind of preparation method of biological decomposing agent | |
| CN105237247A (en) | Silkworm excrement compound bacterial manure and preparing method thereof | |
| CN105400722A (en) | Composite microbial agent, and preparation method and application thereof | |
| CN107032574A (en) | Municipal sludge processing composite bacteria agent and preparation method thereof | |
| CN104152149A (en) | Novel multifunctional composite biological soil improvement agent | |
| CN102964176B (en) | Biological organic fertilizer and preparation method thereof | |
| CN110511894A (en) | A kind of composite bacteria agent and its preparation and application | |
| CN109111310A (en) | A kind of high concentration biogas slurry processing complex microorganism liquid fertilizer production technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170811 |
|
| RJ01 | Rejection of invention patent application after publication |