CN113234616A - Bacillus licheniformis, saline-alkali tolerant microbial agent and preparation method of biomass saline-alkali soil conditioner - Google Patents
Bacillus licheniformis, saline-alkali tolerant microbial agent and preparation method of biomass saline-alkali soil conditioner Download PDFInfo
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Abstract
The invention provides a preparation method of bacillus licheniformis, a saline-alkali resistant microbial inoculum and a biomass saline-alkali soil conditioner, belonging to the technical field of agriculture. The bacillus licheniformis is Bacillus parlicieniformis BCSW-101, and the preservation number is CGMCC No. 19065. The biomass saline-alkali soil conditioner is prepared by the following steps: (1) crushing straws, adjusting the carbon-nitrogen ratio to 25: 1-35: 1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 55-65%, decomposing aerobic compost, airing and drying to obtain decomposed straws; (2) adding saline-alkali resistant microbial inoculum, gamma-polyglutamic acid solution, gamma-polyglutamic acid hydrogel and citric acid into the decomposed straws, uniformly mixing, and granulating to obtain the biomass soil conditioner. The biomass saline-alkali soil conditioner provided by the invention is mainly used for soil improvement, and has the characteristics of being green, degradable and friendly to soil environment.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a preparation method of bacillus licheniformis, a saline-alkali resistant microbial inoculum and a biomass saline-alkali soil conditioner.
Background
Saline-alkali soil is in arid and semiarid area, and the water that contains of bottom soil and groundwater because ground evaporation effect, along with soil capillary action makes the water of containing salinity rise to the top of the earth layer, and the moisture evaporates the back, makes the salinity stay on the soil top layer, gathers and forms. The saline-alkali soil is dry, white, even forms salt crust, is hardened and cracked, has high mineralization and strong corrosivity, can destroy plant cell tissues and obstruct plant growth, and even can directly cause plant root system decay to cause plant death. The problem of soil salinization is one of the most serious environmental problems in the world at present, and under the conditions that the population is continuously increased and cultivated land is gradually reduced, the improvement and utilization of saline-alkali soil have important significance for the sustainable and healthy development of rural economy, the national soil improvement, the ecological environment protection and the like in inland arid agricultural irrigation areas in China.
At present, chemical modifiers (gypsum and the like), industrial wastes (desulphurization slag) and water conservancy measures are mainly adopted to modify the saline-alkali soil, so that the effect of instant effect is achieved, but some uncertain factors which introduce chemical substances and are difficult to degrade and influence the soil exist.
Disclosure of Invention
The invention provides a preparation method of bacillus licheniformis, a saline-alkali resistant microbial inoculum and a biomass saline-alkali soil conditioner. A large amount of waste crop straws are used as main materials, and a saline-alkali-resistant microbial agent and a degradable biological macromolecular material gamma-polyglutamic acid are added, so that a degradable and environment-friendly biomass saline-alkali soil conditioner is developed.
The invention provides a Bacillus licheniformis, which is specifically Bacillus paralicheniformis BCSW-101, and the preservation number is CGMCC No. 19065.
The invention also provides a saline-alkali resistant microbial inoculum which comprises the bacillus licheniformis.
Further, the bacillus subtilis, the bacillus tequilensis and the bacillus megaterium are also included.
Further, Lactobacillus delbrueckii is included.
The invention also provides a preparation method of the saline-alkali resistant microbial inoculum, which comprises the following steps:
(1) respectively selecting a ring of bacillus licheniformis, bacillus subtilis, bacillus tequilensis, bacillus megaterium and lactobacillus delbrueckii from the well-preserved inclined plane, and respectively inoculating the bacillus licheniformis, the bacillus subtilis, the bacillus tequilensis, the bacillus megaterium and the lactobacillus delbrueckii into a liquid culture medium for independent culture for 18-30 h; wherein the mass ratio of the bacillus licheniformis to the bacillus subtilis to the bacillus tequilensis to the lactobacillus delbrueckii is 1:1:1:1: 1;
(2) uniformly mixing the separately cultured bacillus licheniformis, bacillus subtilis, bacillus tequilensis and bacillus megaterium, inoculating the mixture in an optimized culture medium for culturing for 18-30h by the inoculation amount of 1-5% (v/v) to obtain a composite bacillus liquid;
(3) and (3) mixing the composite bacillus liquid obtained in the step (2) with the lactobacillus delbrueckii liquid obtained in the step (1) in a mass ratio of 1:0.5-1:2 to obtain the composite microbial agent.
The invention also provides a biomass saline-alkali soil conditioner which comprises any one of the saline-alkali tolerant microbial inoculum.
The invention also provides a preparation method of the biomass saline-alkali soil conditioner, which comprises the following steps:
(1) crushing straws, adjusting the carbon-nitrogen ratio to 25: 1-35: 1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 55-65%, decomposing aerobic compost, airing and drying to obtain decomposed straws;
(2) adding saline-alkali resistant microbial inoculum, gamma-polyglutamic acid solution, gamma-polyglutamic acid hydrogel and citric acid into the decomposed straws, uniformly mixing, and granulating to obtain the biomass saline-alkali soil conditioner.
Furthermore, in the step (1), the time for decomposing the aerobic compost is 30-40 days.
Further, in the step (2), the decomposed microbial inoculum is an organic fertilizer starter;
in the step (2), the following components are added in parts by weight: 0.5-15.0 parts of saline-alkali resistant microbial inoculum, 0.5-1.5 parts of gamma-polyglutamic acid solution, 0.5-15.0 parts of gamma-polyglutamic acid hydrogel and 0.1-0.6 part of citric acid are added into 600 parts of decomposed straws.
Further, in the step (2), the concentration of the gamma-polyglutamic acid solution is 20-40 g/L.
The invention also provides a biomass saline-alkali soil conditioner prepared by the preparation method of the biomass saline-alkali soil conditioner.
The invention has the following advantages:
the biomass saline-alkali soil conditioner provided by the invention is prepared by compounding decomposed crop straws, a saline-alkali resistant microbial agent and a degradable biological macromolecular material gamma-polyglutamic acid, wherein the decomposed straws can provide organic carbon for the propagation and growth of the saline-alkali resistant microbial agent, the saline-alkali resistant microbial agent adopts the combined action of Bacillus licheniformis paralicheniformis BCSW-101, Bacillus subtilis, Bacillus megaterium and lactobacillus delbrueckii, organic acid is generated in the growth and propagation processes of the saline-alkali resistant microbial agent, and the gamma-polyglutamic acid has the functions of keeping soil moisture and enhancing fertilizer efficiency.
Compared with the existing chemical modifier, the biomass soil modifier provided by the invention has the characteristics of being green, degradable and friendly to soil environment, and can obviously improve the soil microbial biomass, the soil enzyme activity, the soil pH value and the total salt content as well as the plant height and the plant diameter.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
The invention provides a Bacillus licheniformis, which is specifically Bacillus paralicheniformis BCSW-101, and the preservation number is CGMCC No. 19065.
In the invention, Bacillus licheniformis BCSW-101 is obtained by separating and screening from saline-alkali soil, the strain is domesticated and cultivated for a plurality of times, and the domesticated fruiting bodies are divided into components and are preserved in the common microorganism center of China general microbiological culture Collection Center (CCM) within 12 months and 2 days in 2019, wherein the address is No. 3 of Xilu No.1 of Beijing city rising area, the institute of microbiology of China academy of sciences, zip code 100101, the preservation number is CGMCC No.19065, and the classification names are as follows: bacillus paralicheniformis.
The embodiment of the invention also provides a saline-alkali tolerant microbial inoculum which comprises any one of the bacillus licheniformis.
In an embodiment of the invention, the saline-alkali resistant microbial inoculum further comprises bacillus subtilis, bacillus tequilensis and bacillus megaterium.
In an embodiment of the invention, the saline-alkali resistant microbial inoculum further comprises lactobacillus delbrueckii.
Wherein the mass ratio of the bacillus licheniformis, the bacillus subtilis, the bacillus tequilensis, the bacillus megaterium and the lactobacillus delbrueckii is 1:1:1: 1.
The embodiment of the invention also provides a preparation method of the saline-alkali tolerant microbial inoculum, which comprises the following steps:
(1) respectively selecting a ring of bacillus licheniformis, bacillus subtilis, bacillus tequilensis, bacillus megaterium and lactobacillus delbrueckii from the well-preserved inclined plane, and respectively inoculating the bacillus licheniformis, the bacillus subtilis, the bacillus tequilensis, the bacillus megaterium and the lactobacillus delbrueckii into a liquid culture medium for independent culture for 18-30 h; wherein the mass ratio of the bacillus licheniformis to the bacillus subtilis to the bacillus tequilensis to the lactobacillus delbrueckii is 1:1:1:1: 1;
(2) uniformly mixing the separately cultured bacillus licheniformis, bacillus subtilis, bacillus tequilensis and bacillus megaterium, inoculating the mixture in an optimized culture medium for culturing for 18-30h by the inoculation amount of 1-5% (v/v) to obtain a composite bacillus liquid;
(3) and (3) mixing the composite bacillus liquid obtained in the step (2) with the lactobacillus delbrueckii liquid obtained in the step (1) in a mass ratio of 1:0.5-1:2 to obtain the composite microbial agent.
Wherein, the liquid culture medium for the independent culture of the bacillus comprises: peptone 1%, yeast extract 0.5%, and sodium chloride 1% (% by mass). pH 7.2.
The liquid culture medium for the single culture of the lactobacillus delbrueckii comprises: 1% of peptone, 1% of beef extract, 0.5% of yeast extract, 0.2% of dipotassium phosphate, 0.2% of diammonium citrate, 0.5% of sodium acetate, 2% of glucose, 800.1% of tween, 0.05% of magnesium sulfate and 0.025% of manganese sulfate (mass percent). The pH was 6.2.
The optimized culture medium comprises: 0.8% of sucrose, 0.75% of yeast extract, 0.25% of urea, 1% of sodium chloride and 0.5% of magnesium sulfate (mass percentage). pH 7.2.
The embodiment of the invention also provides a biomass saline-alkali soil conditioner, which is the saline-alkali tolerant microbial inoculum.
In one embodiment of the invention, the biomass saline-alkali soil conditioner is prepared by the following steps:
(1) crushing straws, adjusting the carbon-nitrogen ratio to 25: 1-35: 1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 55-65%, decomposing aerobic compost, airing and drying to obtain decomposed straws;
(2) adding saline-alkali resistant microbial inoculum, gamma-polyglutamic acid solution, gamma-polyglutamic acid hydrogel and citric acid into the decomposed straws, uniformly mixing, and granulating to obtain the biomass soil conditioner.
In one embodiment of the invention, in the step (1), the time for decomposing the aerobic compost is 30-40 days.
In an embodiment of the invention, in the step (2), the decomposed microbial inoculum is an organic fertilizer starter.
In one embodiment of the invention, in the step (2), the following components are added in parts by weight: 0.5-15.0 parts of saline-alkali resistant microbial inoculum, 0.5-1.5 parts of gamma-polyglutamic acid solution, 0.5-15.0 parts of gamma-polyglutamic acid hydrogel and 0.1-0.6 part of citric acid are added into 600 parts of decomposed straws.
In one embodiment of the present invention, in the step (2), the concentration of the gamma-polyglutamic acid solution is 20-40 g/L.
The present invention will be described in detail with reference to examples.
Example 1A preparation method of a biomass saline-alkali soil conditioner comprises the following steps:
(1) crushing straws, adjusting the carbon-nitrogen ratio to 30:1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 60%, carrying out aerobic composting for 40 days, airing and drying to obtain decomposed straws;
(2) adding saline-alkali resistant microbial inoculum, gamma-polyglutamic acid solution and gamma-polyglutamic acid hydrogel into the decomposed straws, uniformly mixing, and granulating to obtain the biomass soil conditioner.
In the step (2), the decomposed microbial inoculum is an organic fertilizer starter. The organic fertilizer starter is purchased from Luoyang Oucaik Biotechnology GmbH, and the model is high-temperature type.
The saline-alkali resistant microbial inoculum is prepared from bacillus subtilis, bacillus licheniformis, bacillus megaterium and bacillus tequilensis according to the mass ratio of 1:1:1:1 are mixed to obtain the product.
The additive is added according to the following parts by weight: 600 parts of decomposed straw are added with 5 parts of saline-alkali resistant microbial inoculum, 1 part of gamma-polyglutamic acid solution, 10 parts of gamma-polyglutamic acid hydrogel and 0.2 part of citric acid.
Example 2A preparation method of a biomass saline-alkali soil conditioner comprises the following steps:
(1) crushing straws, adjusting the carbon-nitrogen ratio to 35:1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 58%, carrying out aerobic composting for 35d, airing and drying to obtain decomposed straws;
(2) adding saline-alkali resistant microbial inoculum, gamma-polyglutamic acid solution and hydrogel into the decomposed straw, uniformly mixing, and granulating to obtain the biomass soil conditioner.
In the step (2), the decomposed microbial inoculum is an organic fertilizer starter. The organic fertilizer starter is purchased from Luoyang Oucaik Biotechnology GmbH, and the model is high-temperature type.
The saline-alkali resistant microbial inoculum is prepared from bacillus subtilis, bacillus licheniformis, bacillus megaterium and bacillus tequilensis according to the mass ratio of 1:1:1:1 are mixed to obtain the product.
The additive is added according to the following parts by weight: 600 parts of decomposed straw are added with 1 part of saline-alkali resistant microbial inoculum, 0.5 part of gamma-polyglutamic acid solution, 5 parts of gamma-polyglutamic acid hydrogel and 0.5 part of citric acid.
Comparative example 1A preparation method of a decomposed straw soil conditioner comprises the following steps:
same as in step (1) of example 1.
The method specifically comprises the following steps: crushing the straws, adjusting the carbon-nitrogen ratio to 30:1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 60%, carrying out aerobic composting for 40 days, airing and drying to obtain the decomposed straw soil conditioner.
Comparative example 2Chemical soil conditioner
The furfural residues (purchased from flourishing cattle agricultural development limited, Hebei) and the gypsum powder (purchased from synthetic gypsum factory in Quvo county, Shaanxi province) are mixed according to the mass ratio of 1: 1.
Comparative example 3A living creatureSoil conditioner
Biomass soil amendment (from luoyang europe becker biotechnology gmbh).
Test example 1A method for planting hot pepper by using a biomass saline-alkali soil conditioner comprises the following steps:
before pepper planting, the biomass saline-alkali soil conditioner obtained in example 1 and comparative examples 1-3 is turned into soil together with a base fertilizer according to the mass of the biomass soil conditioner required by 1 mu of saline-alkali soil being 120kg, then pepper planting is carried out for 5 months, after harvesting for 10 months, the influences of the conditioner on soil microbial biomass, soil enzyme activity, soil pH value and total salt content as well as on pepper plant height and diameter are investigated, and the results are shown in tables 1-4.
TABLE 1 Effect on microbial load in soil 0-20cm
TABLE 2 Effect on the enzyme activity of soil 0-20cm
TABLE 3 Effect on soil pH and Total salt content of 0-20cm
TABLE 4 influence on plant height and diameter of Capsici fructus
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The Bacillus licheniformis is particularly Bacillus paralicheniformis BCSW-101, and the preservation number is CGMCC No. 19065.
2. A saline-alkali tolerant microbial inoculant comprising the bacillus licheniformis of claim 1.
3. The saline-alkali tolerant microbial inoculum according to claim 2, further comprising bacillus subtilis, bacillus tequilensis and bacillus megaterium.
4. The saline-alkali tolerant microbial inoculum according to claim 3, further comprising Lactobacillus delbrueckii.
5. The method for preparing the saline-alkali tolerant microbial inoculum according to any one of claims 2 to 4, comprising the following steps:
(1) respectively selecting a ring of bacillus licheniformis, bacillus subtilis, bacillus tequilensis, bacillus megaterium and lactobacillus delbrueckii from the well-preserved inclined plane, and respectively inoculating the bacillus licheniformis, the bacillus subtilis, the bacillus tequilensis, the bacillus megaterium and the lactobacillus delbrueckii into a liquid culture medium for independent culture for 18-30 h; wherein the mass ratio of the bacillus licheniformis to the bacillus subtilis to the bacillus tequilensis to the lactobacillus delbrueckii is 1:1:1:1: 1;
(2) uniformly mixing the separately cultured bacillus licheniformis, bacillus subtilis, bacillus tequilensis and bacillus megaterium, inoculating the mixture in an optimized culture medium for culturing for 18-30h by the inoculation amount of 1-5% (v/v) to obtain a composite bacillus liquid;
(3) and (3) mixing the composite bacillus liquid obtained in the step (2) with the lactobacillus delbrueckii liquid obtained in the step (1) in a mass ratio of 1:0.5-1:2 to obtain the composite microbial agent.
6. A biomass saline-alkali soil conditioner comprising the saline-alkali tolerant microbial inoculum according to any one of claims 2 to 4.
7. The preparation method of the biomass saline-alkali soil amendment of claim 6, which is characterized by comprising the following steps:
(1) crushing straws, adjusting the carbon-nitrogen ratio to 25: 1-35: 1 by using urea or cow dung, applying a decomposing microbial inoculum, adjusting the water content to 55-65%, decomposing aerobic compost, airing and drying to obtain decomposed straws;
(2) adding saline-alkali resistant microbial inoculum, gamma-polyglutamic acid solution, gamma-polyglutamic acid hydrogel and citric acid into the decomposed straws, uniformly mixing, and granulating to obtain the biomass saline-alkali soil conditioner.
8. The production method according to claim 7,
in the step (1), the time for decomposing the aerobic compost is 30-40 days.
9. The production method according to claim 1,
in the step (2), the decomposed microbial inoculum is an organic fertilizer starter;
in the step (2), the following components are added in parts by weight: 0.5-15.0 parts of saline-alkali resistant microbial inoculum, 0.5-1.5 parts of gamma-polyglutamic acid solution, 0.5-15.0 parts of gamma-polyglutamic acid hydrogel and 0.1-0.6 part of citric acid are added into 600 parts of decomposed straws.
10. The production method according to claim 1,
in the step (2), the concentration of the gamma-polyglutamic acid solution is 20-40 g/L.
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| CN113826618A (en) * | 2021-08-16 | 2021-12-24 | 天津坤禾生物科技集团股份有限公司 | Agricultural microbial hydrogel agent with disease resistance and yield increase |
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| CN113826618A (en) * | 2021-08-16 | 2021-12-24 | 天津坤禾生物科技集团股份有限公司 | Agricultural microbial hydrogel agent with disease resistance and yield increase |
| CN113582762A (en) * | 2021-08-19 | 2021-11-02 | 宁夏农林科学院农业资源与环境研究所（宁夏土壤与植物营养重点实验室） | Compound microbial fertilizer special for saline-alkali soil alfalfa and preparation method thereof |
| CN114107130A (en) * | 2021-12-16 | 2022-03-01 | 天津科技大学 | Compound microbial fertilizer and preparation method and application thereof |
| CN114107130B (en) * | 2021-12-16 | 2024-03-15 | 天津北洋百川生物技术有限公司 | Composite microbial fertilizer and preparation method and application thereof |
| CN114703087A (en) * | 2022-02-09 | 2022-07-05 | 上海圣珑环境修复材料有限公司 | Bacillus licheniformis fermentation culture medium, biological preparation and application of biological preparation |
| CN114854422A (en) * | 2022-03-14 | 2022-08-05 | 河北工程大学 | Saline-alkali soil conditioner rich in salt-tolerant microorganisms and preparation method and device thereof |
| CN114854422B (en) * | 2022-03-14 | 2023-05-23 | 河北工程大学 | Saline-alkali soil conditioner rich in salt-tolerant microorganisms and preparation method and device thereof |
| CN114875085A (en) * | 2022-05-09 | 2022-08-09 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Liquid fermentation method for increasing yield of gamma-polyglutamic acid |
| CN114875085B (en) * | 2022-05-09 | 2024-02-02 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Liquid fermentation method for improving gamma-polyglutamic acid yield |
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