CN107299066A - The preparation method and degradation process method of a kind of microbial degradation liquid of oily sludge - Google Patents
The preparation method and degradation process method of a kind of microbial degradation liquid of oily sludge Download PDFInfo
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- CN107299066A CN107299066A CN201710476370.6A CN201710476370A CN107299066A CN 107299066 A CN107299066 A CN 107299066A CN 201710476370 A CN201710476370 A CN 201710476370A CN 107299066 A CN107299066 A CN 107299066A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
- C02F2101/327—Polyaromatic Hydrocarbons [PAH's]
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Abstract
The invention discloses a kind of preparation method of the microbial degradation liquid of oily sludge and degradation process method, the preparation method comprises the following steps：S1：The culture medium of preparing microorganism flora first；S2：Complex microbial community is cultivated in step S1 first culture medium, the first microflora degradation liquid is obtained；S3：The culture medium of preparing microorganism flora second；S4：The first microflora degradation liquid is inoculated in second culture medium and cultivated, the second microflora degradation liquid is obtained；S5：Using the second microflora degradation liquid come oily sludge of degrading, microbial degradation processing is completed.Methods described is selected by unique microorganism species, cultural method selects composite portfolio and the collaboration that multiple technical characteristics are operated etc. with degradation technique, so as to achieve good degradation effect, had a good application prospect and industrial production potential in environmental improvement method.
Description
Technical field
The present invention relates to a kind of processing method of oily sludge, relate more particularly to a kind of microbial degradation processing of oily sludge
Method, belongs to environmental protection and prevention and cure of pollution and improvement field.
Background technology
At present, as the oil exploitation up to decades and crude oil are processed, in oil exploration, exploit, process, transport, use
During be all inevitably generated oily sludge.In these oily sludges, all containing substantial amounts of alkane, aromatic hydrocarbon, asphalitine,
The multiple pollutants such as non-hydrocarbon compound, so that serious influence is caused to environment, especially for the shadow of soil and groundwater
Sound is the most notable.Therefore, how while reasonable utilize, oily sludge is handled, in being current field of environment protection
An important content and research topic.Wherein, a very important processing means are exactly microbial degradation processing, this method
There is a more significant advantage relative to other processing methods, such as it is innoxious, without burying and burning etc., to the shadow of environment
Ring minimum.Such a consideration is based on, people have carried out substantial amounts of further investigation for microbial degradation processing oily sludge, and
Many achievements are achieved, for example：CN1357617A discloses condensed oil polluted soil degrading bacteria and application method in a kind of oil, its
Belong to fungi, entitled Fusarium (Fcusarium Lk.) is preserved in the common micro- life of China Committee for Culture Collection of Microorganisms
Thing center (CGMCC No.：0498), application method is added in condensed oil polluted soil for above-mentioned bacterium solution is connect into bacterium amount by 2-4%,
Nitrogen, phosphorus are added, carbon is controlled:Nitrogen:The ratio of phosphorus is 100:5-10:In the range of 0.5-5, humidity 15-25%, temperature 25-35 are kept
DEG C, pH is 6-7, is regularly stirred to degraded standard.Its degradability is strong, and speed is fast, be easy to breeding culture.CN101066829A is public
A kind of Comprehesive biodegrading process of oil-containing sludge is opened, first, according to sand:String:Organic fertilizer=1:1:1
Ratio, be sufficiently mixed, prepare fermentation auxiliary material it is standby.Meanwhile, by special scientific research institution to oil present in oily sludge
Alkane degradation flora is purified, expands numerous, and petroleum hydrocarbon degradation microbial inoculum (it is required that efficient bacterium is no less than 12 × 109/g) is made.
The first step, heap is rotten.I.e. fermentation auxiliary material and oily sludge according to 1:1 ratio is sufficiently mixed, and is carrying out ground Anti-seeping technology
Place on bank up, manual compaction.In the weather of ground temperature >=15 DEG C, heap is rotten 10 days.Second step, microbial degradation：By
Paved through completing the rotten oily sludge of heap by thick 30 cm, with 1% dispensing microbial inoculum of heap actual weight of oily sludge after rotten, stirring
Uniformly.Spray water once sooner or later daily.Turn over once within every three days.At least continue more than 30 days, until visually to judge that ashen switchs to
Yellowish-brown, and there is no crude oil smell.3rd step, plant promotes to decompose.Planted on the oily sludge for completing the first two steps
The local suitable plant variety of plant, such as Festuca Arundinacea, annual bluegrass, corn, the purpose is to utilize root system of plant further to oil-containing
Harmful components in sludge carry out absorption decomposition, are finally reached the discharge standard of the provisions of the relevant regulations issued by the State.CN101603018A is disclosed
A kind of degraded oil, restoring petroleum polluted soil ecology bacteria preparation and preparation method thereof.Degraded oil, remedying oil-polluted soil
The ecological bacteria preparation of earth is by the optimized combination of bacillus megaterium, Pseudomonas fluorescens, streptococcus fecalis and candida tropicalis
The proportioning of the composite flora of composition, wherein each component is 1-2:1:1:0.5-1, its good effect is：Bacteria preparation is manured into soil
After can quickly form Tiny ecosystem dominant microflora.If adding the 2-5% bacterium relative to surface soil (cultivating layer, about 15-20 cm) weight
Agent, oil removal rate is more than effective clump count height of 75% (methylene chloride reflux is extracted, gravimetric method) bacteria preparation, adaptability in three months
By force, there is obvious effect to restoring petroleum polluted soil ecology environment.CN102464438A discloses one kind and utilizes microorganism
The method of degraded well site oily sludge；By cultivating, separating, screen, tame, lure from the well site of oil field oily sludge of Loess Tableland
Become, obtain Pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis, 4 kinds of degradation bacterias of Acinetobacter junii, be 1 according to weight ratio:
1:1:1 is mixed into oily sludge degrading microorganism flora；Initial pH value=6, carbon weight nitroxide ratio is equal to 100:3, carbon phosphorus weight ratio etc.
In 1000:0.6, laboratory adds the mass concentration of system for handling petrochina class of Mixed Microbes from 17214 mg/kg in 72 h
1257 mg/kg are down to, degradation rate is 92.7%；Pass through the place of 56 days to the oily sludge that initial oil content is 10.55% in well site
Reason experiment, petroleum substance clearance is up to 89.1%, and treatment effect is obvious.CN102485673A discloses a kind of suitable raising
The microbial nutrition formula of Sludge in Oilfields biological degradation rate：It is molten for 500-700 ppm ammonium chloride in every mass per liter concentration
In liquid following material is added by mass concentration：Nitrogen content：500-700 ppm, phosphorus content：100-120ppm, potassium content： 50-90
Ppm, content of magnesium：10 ppm, calcium content 8-12 ppm, sulfur content：15 ppm, manganese content：1-4 ppm, iron content：1 ppm, copper
Content：0.5 ppm, non-ionic surfactant：1250 ppm, cobalt content：5-10 ppm, Zn content：5-10 ppm, boron
Content：5-10 ppm and molybdenum content：5-10 ppm；Disposal of oily sludge is carried out using it, oil content can be down to less than 0.3%, in fact
Existing qualified discharge.CN104450597A discloses a kind of preparation method of oil degradation bacteria solid fungicide, comprises the following steps that：
1st, the screening of oil degradation bacteria, domestication, 2, prepare seed culture fluid, 3, the fermentation of solid fungicide, 4, composting product drying,
Efflorescence, metering, packaging.Effect is obtained below：Using nonionic surfactant Tween-80 as solubilizer, Neng Gouti
The solute effect of high oil.Solid fermentation raw materials used in patent of the present invention are easy to get, technique is simpler, and solid fungicide contains a large amount of carbon
Element and nutrient, more suitably matrix is provided for bacterial growth, strong to microorganism affinity, and immobilization efficiency is high.Improve institute
Add the competitiveness and degradation efficiency of microorganism and indigenous microorganism.Solid fungicide is readily transported and agricultural operation, it is adaptable to stone
The extensive biology in situ reparation of soil contaminated by crude oil.CN102533578A discloses a kind of microbial inoculum for degrading polycyclic aromatic hydrocarbons,
It is made up of 2 kinds of bacterial strains such as pale yellow mycobacteria P6, micro- acidophilus oligotrophic monad P56.The microbial inoculum have degraded soil and
The ability of a variety of polycyclic aromatic hydrocarbons of sewage, with repair polycyclic aromatic hydrocarbon pollution remarkable result, its degrade in the solution (pyrene and
Fluoranthene) polycyclic aromatic hydrocarbon ability be more than 90%, with good application prospect.CN102745821A discloses a kind of for dirt
Complex micro organism fungicide of mud decrement and its preparation method and application, complex micro organism fungicide be by containing Pseuomonas denitrifican,
Norcardia corailina, candida utili, hydrogenlike silicon ion and the fermented liquid bacterial agent being made of Aspergillus sojae.Utilize microorganism
Mutuality between flora, all very high biodegradable systems of various enzymatic activitys needed for forming one, effectively can destroy and divide
Solve the thalline of dead in activated sludge or aging, at the same can decomposing organic matter, in the case where not influenceing effluent quality, pass through
Process decrement to sludge reaches the generating capacity of reduction excess sludge.Larger change now is being not required to using the complex micro organism fungicide
On the premise of row sewage treatment process, mud decrement rate in sewage disposal process is set to reach 30-70%.Reduce the comprehensive of Treatment of Sludge
Operating cost is closed, there is great meaning in terms of economy, environmental and social benefits.CN103567220A discloses a kind of oil
The microorganism in situ restorative procedure of contaminated soil, its step is：1st, bacteria group culture；2nd, flora is activated；3rd, microorganism growth adds
Fast agent is prepared；4th, compounding；5th, biosurfactant is prepared；6th, just throw；7th, repair.The oil-polluted soils
Microorganism in situ restorative procedure in, by petroleum microorganism degradation flora, microorganism Acceleration of growth agent work, biosurfactant
For main component, again supplemented with certain microbial nutrition element, preferred petroleum microorganism conversion flora is put into soil
Meanwhile, increase microorganism Acceleration of growth agent promotes its high speed to breed, thus greatly improve the conversion of soil petrochina element with
Degradation efficiency, the problem of solving time length in oil-polluted soils microorganism in situ restorative procedure, slowly effect, makes microorganism
Based technique for in-situ remediation turns into reliable, efficient, exercisable oil-polluted soils governing measure.CN103667058A discloses one kind
The microbial composite and its processing method of chloride polycyclic aromatic hydrocarbon in degraded greasy filth, the microbial composite include following weight ratio
Microorganism：Sphingol single-cell is moved less：Sphingol single-cell：Oligotrophic unlinks bacterium for 1:0.5-1.5:0.5-1.5.Also provide
The processing method of contaminated soil is carried out using the microbial composite of chloride polycyclic aromatic hydrocarbon in greasy filth of degrading, this method is including being contained
Greasy dirt adds microbial bacterial agent and nutrient solution, is less than 0.5% to oil content after processing.This method is from degrading polycyclic aromatic hydrocarbons and miscellaneous
Ring class material sets out, and separates and has cultivated three kinds of microorganisms for having obvious effect to this kind of environmental toxicants, have studied this
Quasi-microorganism plays the necessary nutritional ingredient needed for degrading activity, improves its degraded to polycyclic aromatic hydrocarbon and heterocyclic material
Effect.CN103755039A discloses a kind of application of complex microbial inoculum in processing petrifaction sewage sludge.Specially：
1st, bio-carrier is prepared：Glutaraldehyde suspension stirring is added in polyphenylene oxide, the PBS containing CaCl2 is added after washing and is delayed
Fliud flushing is stood；2nd, the expansion culture of complex microbial inoculum；3rd, the microbial composite bacteria after the expansion culture for obtaining step 2
Agent is put into the bio-carrier of step 1 preparation；4th, the bio-carrier for being loaded with complex microbial inoculum that step 3 is obtained is added
In petrifaction sewage, complex microbial inoculum continued to rise in value to stationary phase, and biomembrane is formed on bio-carrier surface, carried out new old generation
Thank, adsorb, absorb, digesting, decomposing organic pollution and heavy metal in petrifaction sewage and sludge, be allowed to transform into stable nothing
Evil compound matter.Found by studying, the complex microbial inoculum can handle petrifaction sewage sludge very well.CN104031870A is disclosed
A kind of complex microbial inoculum, and it is related to the complex microbial inoculum and biosurfactant constitutes combines reparation
Agent, and the application of the complex microbial inoculum and the joint renovation agent in remedying oil-polluted soils, belong to oil
Contaminated soil or oily sludge recovery technique field；Technical problem to be solved is to provide one kind being capable of effectively degraded oil dirt
The complex microbial inoculum of soil or TPH pollutants, especially n-alkane, hopance and aromatic hydrocarbon in oily sludge is contaminated, together
When the method that the complex microbial inoculum combines remedying oil-polluted soils with biosurfactant is provided.CN106190891A
Disclose a kind of based on the oxidation of sodium peroxydisulfate compound and the biological reinforced Combined Treatment soil with serious petroleum pollution of microorganism species
Or the method for greasy filth, used cooperatively first with sodium peroxydisulfate compound as oxidant and surfactant to contaminated soil or oil
Mud carries out oxidation processes, afterwards double access bacillus (Bacillus sp.) and branch acremonium bacterium (Acremonium
Sp.Y0997) or Phanerochaete chrysosporium (Phanerochaete sp.F0996) mixed bacterial, by chemical oxidation with it is true
Bacterium-bacteria flora biological sequence reinforcing is combined carries out reinforcing Combined Treatment to soil or greasy filth, has been remarkably improved severe oil
The degradation efficiency of contaminated soil or greasy filth, and it is substantially shorter repairing efficiency.As described above, although people have developed microorganism drop
The method of solution processing oily sludge, but for new biodegradation process for treating, still suffer from the necessity for continuing to study and need
Ask, this not still research emphasis and focus in current oily sludge field, where being even more the power that the present invention is accomplished
Leaned on basis.
The content of the invention
In order to research and develop new oily sludge biodegradation process for treating, the present inventor is from practical application, to the method
Carried out substantial amounts of further investigation, after creative work has been paid, so as to complete the present invention, i.e., a kind of oily sludge it is micro-
The preparation method and degradation process method of biodegradable fluid, which raises the technology effect of microbial degradation processing oily sludge
Really.The present invention provides a kind of preparation method of the microbial degradation liquid of oily sludge first, and the preparation method includes following step
Suddenly：S1：The culture medium of preparing microorganism flora first；Configuration step is as follows：
S1-1：By 4-6g yeast extracts, 1-2g beef extracts, 1-2g arginine, 8-12g peptones, 8-12g sodium chloride, 1-3g
Maltose, 4-5g sodium carbonate, 2-4g agar and 8-9g chloride leaches are in the deionized water that 1000 ml temperature are 60-70 DEG C
In, it is sufficiently stirred for, obtains mixed liquor I；S1-2：Into the mixed liquor I, trace element is added, is then sufficiently mixed, so that
To the culture medium of microorganism species first；S2：Complex microbial community is entered in step S1 first culture medium
Row culture, obtains the first microflora degradation liquid；
The complex microbial community configuration step is as follows：
S2-1：By radioresistance acinetobacter calcoaceticus, bacillus subtilis, Acinetobacter lwoffii, Acinetobacter junii, Pseudomonas amygdali
With branch's arthrobacterium according to weight ratio 1:1-2:0.3-0.7:1-1.4:1:2-3 is mixed, so as to obtain complex microorganism
Flora；
S3：The culture medium of preparing microorganism flora second；Configuration step is as follows：
S3-1：1-2 g beef extracts and 4-6 g peptones are added in the mixed liquor I obtained to step S1-1,
It is sufficiently stirred for, obtains mixed liquor I I；S3-2：Into the mixed liquor I I, trace element is added, is then sufficiently mixed, so that
Obtain the culture medium of microorganism species second；S4：The first microflora degradation liquid is inoculated in second culture medium
Row culture, obtains the second microflora degradation liquid i.e. microbial degradation liquid of described oily sludge.
In the step S1-1, yeast extract, beef extract, peptone and agar etc. are all very known materials,
It can be bought and obtained by a variety of commercial channel, is no longer described in detail herein.
In the step S2-1,6 kinds of microbial bacterias are radioresistance acinetobacter calcoaceticus (Acinetobacter
Radioresistens), bacillus subtilis (Bacillus subtilis), Acinetobacter lwoffii (Acinetobacter
Lwoffii), Acinetobacter junii (Acinetobacter junii), Pseudomonas amygdali (Pseudomonas amygdale)
It is very known microbial bacteria with branch arthrobacterium (Arthrobacter ramosus), is no longer gone to live in the household of one's in-laws on getting married one by one herein
State.
It is further preferred that the step S1 specifically includes following steps：
S1-1：By 5g yeast extracts, 1.5g beef extracts, 1.5g arginine, 10g peptones, 10g sodium chloride, 2g maltose,
4.5g sodium carbonate, 3g agar and 8.5g chloride leaches are fully stirred in the deionized water that 1000 ml temperature are 60-70 DEG C
Mix, obtain mixed liquor I；
S1-2：Into the mixed liquor I, trace element water-soluble liquid, the mixed liquor I and the trace element water-soluble liquid are added
Volume ratio is 40:1, it is then sufficiently mixed, so as to obtain the culture medium of microorganism species first；The trace element water-soluble liquid
Be 0.1g sodium molybdates, 0.08g aluminum nitrates, 0.04g zinc gluconates, 0.02g zinc chloride, 0.07g copper sulphate, 0.01g boric acid,
0.12g magnesium nitrates, 0.02g copper chlorides, 0.05g cobalt chlorides, 0.03g manganese chlorides, 0.08g ferrous sulfate, 0.02g stannic chlorides and
0.06g potassium chloride is dissolved in obtained from 1000 ml distilled water.It is further preferred that culture step during the step S2 is specific
It is rapid as follows：
S2-2：The complex microbial community is added in the step S1 culture medium of microorganism species first, in 28-32
Shaken cultivation 20-24 hours at DEG C, so as to obtain the first microflora degradation liquid.
It is further preferred that in the step S2, by weight gram（g）The complex microbial community of meter is with pressing body
Product milliliter（ml）The ratio of the culture medium of the microorganism species first of meter is 1:3000-4000；For example can be 1:3000、 1:
3500 or 1:4000.
It is further preferred that the step S3 specifically includes following steps：
S3-1：1.5 g beef extracts and 5 g peptones are added in the mixed liquor I obtained to step S1-1, are filled
Divide stirring, obtain mixed liquor I I；S3-2：Into the mixed liquor I I, add trace element water-soluble liquid, the mixed liquor I I with
The volume ratio of the trace element water-soluble liquid is 20:1, it is then sufficiently mixed, is cultivated so as to obtain the microorganism species second
Base；The trace element water-soluble liquid be 0.1g sodium molybdates, 0.08g aluminum nitrates, 0.04g zinc gluconates, 0.02g zinc chloride,
0.07g copper sulphate, 0.01g boric acid, 0.12g magnesium nitrates, 0.02g copper chlorides, 0.05g cobalt chlorides, 0.03g manganese chlorides, 0.08g sulphur
Sour ferrous, 0.02g stannic chlorides and 0.06g potassium chloride are dissolved in obtained from 1000 ml distilled water.
Namely：In step S3-1, relative to step S1, increase adds the consumption of beef extract and peptone so that
Beef extract in gained mixed liquor I I is twice in mixed liquor I, and peptone is 1.5 times in mixed liquor I.Namely：
In step S3-2, relative to step S1 (being specifically step S1-2), the consumption of the micro- aqueous solution is entered added by increase so that
Its addition is twice of addition in step S1-2.Inventor has found, so after the content of increase beef cattle medicinal extract and peptone,
Superior technique effect can be obtained, it should be the more nutriments of propagation needs of now microorganism, and so increased
Big content can more adapt to and promote the propagation of microorganism to breed and increase viable bacteria rate, so as to achieve superior technique effect.
And in step S3-2, be similarly increased to the consumption of the trace element water-soluble liquid twice in step S1-2, such as
This also achieves superior technique effect.It should be now micro- content increase, can further promote microorganism species
Propagation breed and increase viable bacteria rate.Wherein, in the step S1 and S3, in mixed liquor I and mixed liquor I I
" I " and " II " is intended merely to refer to the mixed liquor of each step, and (is only code name without specific concrete meaning
).
It is further preferred that the step S4 is specially：At room temperature, according to 1:40-46 volume ratio, by described
One microflora degradation liquid is added in second culture medium, and is warming up to 38- under agitation with 1 DEG C/min of heating rate
40 DEG C, shaken cultivation 5-6 hours at such a temperature, then 48 are warming up to 0.4-0.6 DEG C/min of heating rate again ±
1 DEG C, and cultivate 100-120 minutes at such a temperature, so as to obtain the second microflora degradation liquid i.e. microorganism of oily sludge
Degradation solution.
Then the present invention provides the degradation treatment side of the microbial degradation liquid of the oily sludge obtained by above-mentioned preparation method
Method, progress microbial degradation processing in oily sludge is added to by the second microflora degradation liquid.
Further, above-mentioned degradation process method comprises the following steps：
S5：Using the second microflora degradation liquid come oily sludge of degrading, microbial degradation processing is completed；It is specific as follows：
S5-1：The second microflora degradation liquid is diluted to 10-15 times with deionized water, microflora degradation dilution is obtained；
S5-2：The microflora degradation dilution is added in oily sludge, at 40-50 DEG C of temperature, degraded is sufficiently stirred for
10-20 days, so as to complete the microbial degradation processing of oily sludge.
In the step S5-1, the second microflora degradation liquid is diluted to 10-15 times with deionized water, such as dilute
Release to 10 times, 11 times, 12 times, 13 times, 14 times or 15 times.
It is further preferred that in the step S5-2, the microflora degradation dilution is also same when being added in oily sludge
When add composite assistant, the composite assistant is mass ratio 1:8-10 algae albumen glycolipid and mixing for neopelex
Compound.
It is further preferred that in the step S5-2, with volume milliliter（ml）The microflora degradation dilution of meter
With in terms of dry weight and with quality gram（g）The ratio of the oily sludge of meter is 100:20-30, will every 100 ml microflora degradations dilution
It is added to and is calculated as with dry weight in 20-30 g oily sludge in liquid, for example, can be added to 20 g, 25 g or 30 g with dry
In the oily sludge of restatement.
It is further preferred that the mass ratio of the composite assistant and the oily sludge counted using dry weight is 1:80-120, for example
Can be 1:80、 1:90、 1:100、 1:110 or 1:120.
Wherein, in the step S5-2, the calculating benchmark of the oily sludge be oily sludge in terms of dry weight (i.e.
Quality single-detector is less than 2% drying sludge).That is, carrying out consumption with the microflora degradation dilution and composite assistant
The oily sludge of contrast, be first be scaled quality single-detector less than 2% drying sludge it is (first that oily sludge is abundant
Dry to quality single-detector and be less than 2%, in this, as calculating benchmark), then carry out consumption contrast.
As described above, the invention provides the preparation method and degradation treatment of a kind of microbial degradation liquid of oily sludge
Method, methods described is special by multiple technologies such as unique microorganism species selection, cultural method selection and degradation technique operations
The composite portfolio levied and collaboration, so as to achieve good degradation effect, have a good application prospect in environmental improvement method
And industrial production potential.
Embodiment
Below by specific embodiment, the present invention is described in detail, but the purposes of these exemplary embodiments and
Purpose only be used for enumerate the present invention, not to the present invention real protection scope constitute it is any type of it is any limit, it is more non-will
Protection scope of the present invention is confined to this.
The preparation of trace element water-soluble liquid weighs 0.1g sodium molybdates, 0.08g aluminum nitrates, 0.04g Portugals with electronic balance respectively
Grape saccharic acid zinc, 0.02g zinc chloride, 0.07g copper sulphate, 0.01g boric acid, 0.12g magnesium nitrates, 0.02g copper chlorides, 0.05g chlorinations
Cobalt, 0.03g manganese chlorides, 0.08g ferrous sulfate, 0.02g stannic chlorides and 0.06g potassium chloride, then add above-mentioned various materials
Into 1000ml distilled water, dissolving is sufficiently stirred for completely, so as to obtain trace element water-soluble liquid.Unless otherwise prescribed and explanation,
Otherwise in following all embodiments and comparative example, used trace element water-soluble liquid be it is above-mentioned prepare it is micro
The element aqueous solution.And all room temperatures are 25 DEG C.The S1 of embodiment 1：The culture medium of preparing microorganism flora first, tool
Body comprises the following steps：S1-1：By 5 g yeast extracts, 1.5 g beef extracts, 1.5 g arginine, 10 g albumen
Peptone, 10 g sodium chloride, 2 g maltose, 4.5 g sodium carbonate, 3 g agar and 8.5 g chloride leaches are in 1000
Ml temperature is in 65 DEG C of deionized water, is sufficiently stirred for, obtains mixed liquor I；S1-2：Into the mixed liquor I, add
The volume ratio of trace element water-soluble liquid, the mixed liquor I and the trace element water-soluble liquid is 40:1, it is then sufficiently mixed,
So as to obtain the culture medium of microorganism species first.S2：Complex microbial community is cultivated described the first of step S1
Cultivated in base, obtain the first microflora degradation liquid, specifically include following steps：S2-1：By radioresistance acinetobacter calcoaceticus
(Acinetobacter radioresistens), bacillus subtilis (Bacillus subtilis), Acinetobacter lwoffii
(Acinetobacter lwoffii), Acinetobacter junii (Acinetobacter junii), Pseudomonas amygdali
(Pseudomonas amygdale) and branch's arthrobacterium (Arthrobacter ramosus) compare 1 according to weight:1.5:0.5:
1.2:1:2.5 are mixed, so as to obtain complex microbial community；S2-2：The complex microbial community is added to step
(complex microbial community of gram (g) meter is with pressing volume milliliter (ml) by weight in the S1 culture medium of microorganism species first
The ratio of the culture medium of the microorganism species first of meter is 1:3500), shaken cultivation 22 hours at 30 DEG C, so as to obtain
First microflora degradation liquid.S3：The culture medium of preparing microorganism flora second, specifically includes following steps：S3-1：To step S1-1
1.5 g beef extracts and 5 g peptones are added in the obtained mixed liquor I, are sufficiently stirred for, mixed liquor I I is obtained；
S3-2：Into the mixed liquor I I, trace element water-soluble liquid, the mixed liquor I I and the trace element water-soluble liquid body are added
Product is than being 20:1, it is then sufficiently mixed, so as to obtain the culture medium of microorganism species second.S4：By first flora drop
Solution liquid, which is inoculated in second culture medium, to be cultivated, and is obtained the second microflora degradation liquid, is specially：At room temperature, according to 1:43
Volume ratio, the first microflora degradation liquid is added in second culture medium, and under agitation with 1/DEG C minute
Heating rate be warming up to 39 DEG C, shaken cultivation 5.5 hours at such a temperature, then again with the liter of 0.5/DEG C minute
Warm speed is warming up to 48 DEG C, and cultivates 110 minutes at such a temperature, so as to obtain the second microflora degradation liquid.S5：Make
With the second microflora degradation liquid come oily sludge of degrading, microbial degradation processing is completed, following steps are specifically included：S5-1：
The second microflora degradation liquid is diluted to 12.5 times with deionized water, microflora degradation dilution is obtained；S5-2：By the bacterium
Group's degraded dilution and composite assistant are (for mass ratio 1:9 algae albumen glycolipid and the mixture of neopelex) plus
Enter into oily sludge (by volume milliliter (ml) meter the microflora degradation dilution and in terms of dry weight and in terms of quality gram (g)
Oily sludge ratio be 100:25, the composite assistant and the mass ratio of oily sludge counted using dry weight is 1:100), in temperature
At 45 DEG C of degree, degraded 15 days is sufficiently stirred for, so as to complete the microbial degradation processing of oily sludge.The S1 of embodiment 2：
The culture medium of preparing microorganism flora first, specifically includes following steps：S1-1：By 5 g yeast extracts, 1.5 g beef
Cream, 1.5 g arginine, 10 g peptones, 10 g sodium chloride, 2 g maltose, 4.5 g sodium carbonate, 3 g fine jades
Fat and 8.5 g chloride leaches are sufficiently stirred in the deionized water that 1000 ml temperature are 60 DEG C, obtain mixed liquor
I；S1-2：Into the mixed liquor I, trace element water-soluble liquid, the mixed liquor I and the trace element water-soluble liquid are added
Volume ratio be 40:1, it is then sufficiently mixed, so as to obtain the culture medium of microorganism species first.S2：By complex microorganism
Flora is cultivated in step S1 first culture medium, is obtained the first microflora degradation liquid, is specifically included following steps：
S2-1：By radioresistance acinetobacter calcoaceticus (Acinetobacter radioresistens), bacillus subtilis (Bacillus
Subtilis), Acinetobacter lwoffii (Acinetobacter lwoffii), Acinetobacter junii (Acinetobacter
Junii), Pseudomonas amygdali (Pseudomonas amygdale) and branch's arthrobacterium (Arthrobacter ramosus) are pressed
Compare 1 according to weight:1:0.7:1:1:3 are mixed, so as to obtain complex microbial community；S2-2：By the complex microorganism
Flora is added in the step S1 culture medium of microorganism species first (the complex microbial community that gram (g) is counted by weight
Ratio with the culture medium of the microorganism species first based on volume milliliter (ml) is 1:3000), the shaken cultivation at 28 DEG C
24 hours, so as to obtain the first microflora degradation liquid.S3：The culture medium of preparing microorganism flora second, specifically includes following steps：
S3-1：1.5 g beef extracts and 5 g peptones are added in the mixed liquor I obtained to step S1-1, are fully stirred
Mix, obtain mixed liquor I I；S3-2：Into the mixed liquor I I, add trace element water-soluble liquid, the mixed liquor I I with it is described
The volume ratio of trace element water-soluble liquid is 20:1, it is then sufficiently mixed, so as to obtain the culture medium of microorganism species second.
S4：The first microflora degradation liquid is inoculated in second culture medium and cultivated, the second microflora degradation liquid is obtained, specifically
For：At room temperature, according to 1:40 volume ratio, the first microflora degradation liquid is added in second culture medium, and
Be warming up to 38 DEG C under stirring with the heating rate of 1/DEG C minute, at such a temperature shaken cultivation 6 hours, then again with
The heating rate of 0.4/DEG C minute is warming up to 49 DEG C, and cultivates 120 minutes at such a temperature, so as to obtain described second
Microflora degradation liquid.S5：Using the second microflora degradation liquid come oily sludge of degrading, microbial degradation processing, specific bag are completed
Include following steps：S5-1：The second microflora degradation liquid is diluted to 10 times with deionized water, microflora degradation dilution is obtained
Liquid；S5-2：(it is mass ratio 1 by the microflora degradation dilution and composite assistant:8 algae albumen glycolipid and detergent alkylate
The mixture of sodium sulfonate) be added in oily sludge (by volume milliliter (ml) meter the microflora degradation dilution and with dry weight
The ratio of meter and the oily sludge counted using quality gram (g) is 100:20, the matter of the composite assistant and the oily sludge in terms of dry weight
Amount is than being 1:80), at 40 DEG C of temperature, degraded 10 days is sufficiently stirred for, so as to complete at the microbial degradation of oily sludge
Reason.The S1 of embodiment 3：The culture medium of preparing microorganism flora first, specifically includes following steps：S1-1：5 g yeast are carried
Take thing, 1.5 g beef extracts, 1.5 g arginine, 10 g peptones, 10 g sodium chloride, 2 g maltose, 4.5 g
Sodium carbonate, 3 g agar and 8.5 g chloride leaches are fully stirred in the deionized water that 1000 ml temperature are 70 DEG C
Mix, obtain mixed liquor I；S1-2：Into the mixed liquor I, add trace element water-soluble liquid, the mixed liquor I with it is described
The volume ratio of trace element water-soluble liquid is 40:1, it is then sufficiently mixed, so as to obtain the culture medium of microorganism species first.
S2：Complex microbial community is cultivated in step S1 first culture medium, the first microflora degradation liquid is obtained, is had
Body comprises the following steps：S2-1：By radioresistance acinetobacter calcoaceticus (Acinetobacter radioresistens), bacillus subtilis
Bacterium (Bacillus subtilis), Acinetobacter lwoffii (Acinetobacter lwoffii), Acinetobacter junii
(Acinetobacter junii), Pseudomonas amygdali (Pseudomonas amygdale) and branch's arthrobacterium
(Arthrobacter ramosus) compares 1 according to weight:2:0.3:1.4:1:2 are mixed, so as to obtain complex microorganism
Flora；S2-2：The complex microbial community is added in the step S1 culture medium of microorganism species first (by weight gram
(g) ratio of the complex microbial community of meter and the culture medium of the microorganism species first based on volume milliliter (ml) is
1:4000), shaken cultivation 20 hours at 32 DEG C, so as to obtain the first microflora degradation liquid.S3：Preparing microorganism flora
Two culture mediums, specifically include following steps：S3-1：1.5 g oxen are added in the mixed liquor I obtained to step S1-1
Meat medicinal extract and 5 g peptones, are sufficiently stirred for, obtain mixed liquor I I；S3-2：Into the mixed liquor I I, trace element is added
The volume ratio of the aqueous solution, the mixed liquor I I and the trace element water-soluble liquid is 20:1, it is then sufficiently mixed, so as to obtain institute
State the culture medium of microorganism species second.S4：The first microflora degradation liquid is inoculated in second culture medium and cultivated,
The second microflora degradation liquid is obtained, is specially：At room temperature, according to 1:46 volume ratio, the first microflora degradation liquid is added
Into second culture medium, and 40 DEG C are warming up to the heating rate of 1/DEG C minute under agitation, at such a temperature
Shaken cultivation 5 hours, is then warming up to 47 DEG C, and cultivate at such a temperature with the heating rate of 0.6/DEG C minute again
100 minutes, so as to obtain the second microflora degradation liquid.S5：Using the second microflora degradation liquid come oily sludge of degrading,
Microbial degradation processing is completed, following steps are specifically included：S5-1：The second microflora degradation liquid is diluted to deionized water
15 times, obtain microflora degradation dilution；S5-2：(it is mass ratio 1 by the microflora degradation dilution and composite assistant:10
The mixture of algae albumen glycolipid and neopelex) be added in oily sludge (by volume milliliter (ml) in terms of it is described
The ratio of microflora degradation dilution and the oily sludge counted and counted using quality gram (g) using dry weight is 100:30, the composite assistant with
The mass ratio for the oily sludge counted using dry weight is 1:120), under temperature 50 C, degraded 20 days is sufficiently stirred for, so as to complete
The microbial degradation processing of oily sludge.Comparative example 1-3 comparative examples 1：Step S1-S2, S3-2 and S4-S5 are with real
Example 1 is applied, it is the mixed liquor I in the step S1-1 of embodiment 1 to differ only in the mixed liquor I I in step S3-1
(not adding extra 1.5 g beef extracts and 5 g peptones further).Comparative example 2：Step S1-S2, S3-
1 and S4-S5 be the same as Examples 2, differ only in the body of the mixed liquor I I and trace element water-soluble liquid in step S3-2
Product is than being 40:1 (the addition of trace element water-soluble liquid is not increased and is twice).Comparative example 3：Step S1-S2 and
S4-S5 be the same as Examples 3, differ only in the step S3-1 in step S3 with comparative example 1, and step S-2 is with contrast
(i.e. mixed liquor I I in step S3-1 is the mixed liquor I in the step S1-1 of embodiment 3, step S3-2 to example 2
In the volume ratio of mixed liquor I I and trace element water-soluble liquid be still 40:1, in more detail, it is described that step S3 is obtained
The culture medium of microorganism species second is the culture medium of the microorganism species first in step S1).4-6 pairs of comparative example
Ratio 4：Step S1-S2, S3 and S5 be the same as Example 1, differs only in step S4, is specially：At room temperature, according to
1:43 volume ratio, the first microflora degradation liquid is added in second culture medium, and under agitation with 1/DEG C
The heating rate of minute is warming up to 48 DEG C, and cultivates 458 minutes at such a temperature, so as to obtain second microflora degradation
Liquid is (i.e. without culture under outlet temperature in the first stage 5.5 hours, but when cultivating total under the outlet temperature of second stage
Between, include the time needed for second stage heating in embodiment 1 total time).Comparative example 5：Step S1-S2, S3
With S5 be the same as Examples 2, step S4 is differed only in, is specially：At room temperature, according to 1:40 volume ratio, by described first
Microflora degradation liquid is added in second culture medium, and is warming up to 38 under agitation with the heating rate of 1/DEG C minute
DEG C, shaken cultivation 507.5 minutes (only cultivates total time under outlet temperature, when this is total in the first stage at such a temperature
Between include time needed for the heating of second stage in embodiment 2).Comparative example 6：Step S1-S2, S3 and S5 are with real
Example 3 is applied, step S4 is differed only in, is specially：At room temperature, according to 1:46 volume ratio, by first microflora degradation
Liquid is added in second culture medium, and is warming up to 40 DEG C under agitation with the heating rate of 1/DEG C minute, then
47 DEG C are warming up to the heating rate of 0.6/DEG C minute again, and is cultivated 400 minutes at such a temperature, so as to obtain institute
State the second microflora degradation liquid and (only cultivate total time under second stage outlet temperature, the total time is the in embodiment 3
The incubation time of single order segment endpoint temperature and the incubation time sum of second stage outlet temperature).Comparative example 7-9 comparative examples
7：Step S1-S4 be the same as Examples 1, differ only in and do not add the composite assistant in step S5-2.Comparative example 8：Step
Rapid S1-S4 be the same as Examples 2, differ only in the composite assistant in step S5-2 and replace with single component algae albumen
Glycolipid (its consumption is the same amount of original composite assistant).Comparative example 9：Step S1-S4 be the same as Examples 2, difference only exists
The composite assistant in step S5-2 replaces with single component neopelex, and (its consumption helps for original be combined
The same amount of agent).Degradation property tests 1, for the oil content in the degradation property sludge to be tested containing petroleum sludge
For 30 mg/kg, degraded, after the completion of degraded, measured again dirty according to the method for above-described embodiment and comparative example respectively
Oil content in mud, petroleum degradation rate has been obtained so as to calculate, and concrete outcome see the table below 1.
Wherein, for embodiment 1-3 degradation rate, " for 94.5,93.7,94.1 ", its implication refers to embodiment
1 degradation rate is that the degradation rate of 94.5%, embodiment 2 is that the degradation rate of 93.7%, embodiment 3 is 94.1%.It is other
Class likelihood data also has so mutual corresponding relation, no longer repeats one by one below., as can be seen here：1st, implementation of the invention
Example 1-3 has excellent petroleum degradation rate；2 and without additionally adding beef extract and peptone in the step S3-1,
Or when there is no the consumption for increasing trace element water-soluble liquid in step S3-2, all petroleum degradation rate will be caused to have significant drop
Low (see comparative example 1-2)；And when not increasing the consumption of this three simultaneously, degradation rate have reduction the most significant (see pair
Ratio 3).Increase three consumption can use to obtain unexpected technique effect while this proves such；3rd, for step S4
Two-part heating culture, can obtain best technique effect, and when changing any one technical characteristic, will all cause drop
Solution rate is significantly reduced (see comparative example 4-6)；4th, the composite assistant in step S5, which is used, can also significantly affect final skill
Art effect, when using any single component when, will all cause to be significantly reduced, and, it is surprising that when without using
During any auxiliary agent, degradation rate is higher than only using only degradation rate during neopelex (see comparative example 7 and 9 on the contrary
Contrast), this proves only to be used only neopelex on the contrary without any improvement, and only simultaneously using compound
Unexpected synergy has been played between auxiliary agent, two kinds of components, best degradation rate is achieved.
, for the degradation property of condensed-nuclei aromatics
The sludge containing condensed-nuclei aromatics compound is prepared, is specially：Naphthalene content is 50 mg/kg, and it is 45 that benzo [a] pyrene, which contains,
Mg/kg and fluoranthene content are 62 mg/kg.Degraded, degraded according to the method for above-described embodiment and comparative example respectively
Cheng Hou, measures the content of each condensed-nuclei aromatics compound in sludge, its respective degradation rate has been obtained so as to calculate, have again
Body result see the table below 2.
Wherein, by taking the degradation rate of naphthalene as an example, embodiment 1-3 degradation rate is " 95.6-96.3% ", and its implication refers to reality
Apply a 1-3 to be located within 95.6-96.3% interval the degradation rate of naphthalene, other same expression ways are also so to refer to
For implication, no longer list one by one.
As can be seen here, microbial degradation method of the invention has very high drop for the condensed-nuclei aromatics in oily sludge
Solution rate, for toxicity very big benzo [a] pyrene, degradation effect is very good (same to the degradation rule of condensed-nuclei aromatics
Table 1, is no longer repeated herein).So as to industrially have a good application prospect and industrializing implementation potentiality.
As described above, the invention provides a kind of biodegradation process for treating of oily sludge, methods described passes through only
Composite portfolio and the collaboration that multiple technical characteristics are operated etc. with degradation technique are selected in special microorganism species selection, cultural method, from
And good degradation effect is achieved, had a good application prospect and industrial production potential in environmental improvement method.
Although for the purpose illustrated and described, and describing the above-mentioned embodiment of the present invention, these are not detailed
Most description, can not scope of the invention is limited to this.It will be understood by those skilled in the art that can be to above-mentioned reality of the invention
The mode of applying makes numerous modifications and variations, and these all modifications and/or change are included in such as the claim of the present invention
Within limited range, the scope and spirit of the present invention limited without departing from claim as described.
Claims (10)
1. the preparation method of the microbial degradation liquid of a kind of oily sludge, it is characterised in that the preparation method includes following step
Suddenly： S1：The culture medium of preparing microorganism flora first；Configuration step is as follows：
S1-1：By 4-6g yeast extracts, 1-2g beef extracts, 1-2g arginine, 8-12g peptones, 8-12g sodium chloride, 1-3g
Maltose, 4-5g sodium carbonate, 2-4g agar and 8-9g chloride leaches are in the deionized water that 1000 ml temperature are 60-70 DEG C
In, it is sufficiently stirred for, obtains mixed liquor I； S1-2：Into the mixed liquor I, trace element is added, is then sufficiently mixed, so that
Obtain the culture medium of microorganism species first； S2：By complex microbial community in step S1 first culture medium
Cultivated, obtain the first microflora degradation liquid；
The complex microbial community configuration step is as follows：
S2-1：By radioresistance acinetobacter calcoaceticus, bacillus subtilis, Acinetobacter lwoffii, Acinetobacter junii, Pseudomonas amygdali
With branch's arthrobacterium according to weight ratio 1:1-2:0.3-0.7:1-1.4:1:2-3 is mixed, so as to obtain complex microorganism
Flora；
S3：The culture medium of preparing microorganism flora second；Configuration step is as follows：
S3-1：1-2 g beef extracts and 4-6 g peptones are added in the mixed liquor I obtained to step S1-1,
It is sufficiently stirred for, obtains mixed liquor I I； S3-2：Into the mixed liquor I I, trace element is added, is then sufficiently mixed, from
And obtain the culture medium of microorganism species second； S4：The first microflora degradation liquid is inoculated in second culture medium
Cultivated, obtain the second microflora degradation liquid i.e. microbial degradation liquid of described oily sludge.
2. the preparation method of the microbial degradation liquid of oily sludge according to claim 1, it is characterised in that the step
S1 specifically includes following steps：
S1-1：By 5g yeast extracts, 1.5g beef extracts, 1.5g arginine, 10g peptones, 10g sodium chloride, 2g maltose,
4.5g sodium carbonate, 3g agar and 8.5g chloride leaches are fully stirred in the deionized water that 1000 ml temperature are 60-70 DEG C
Mix, obtain mixed liquor I；
S1-2：Into the mixed liquor I, trace element water-soluble liquid, the mixed liquor I and the trace element water-soluble liquid are added
Volume ratio is 40:1, it is then sufficiently mixed, so as to obtain the culture medium of microorganism species first；The trace element water-soluble liquid
Be 0.1g sodium molybdates, 0.08g aluminum nitrates, 0.04g zinc gluconates, 0.02g zinc chloride, 0.07g copper sulphate, 0.01g boric acid,
0.12g magnesium nitrates, 0.02g copper chlorides, 0.05g cobalt chlorides, 0.03g manganese chlorides, 0.08g ferrous sulfate, 0.02g stannic chlorides and
0.06g potassium chloride is dissolved in obtained from 1000 ml distilled water.
3. the preparation method of the microbial degradation liquid of oily sludge according to claim 1, it is characterised in that the step
Incubation step is as follows during S2 is specific：
S2-2：The complex microbial community is added in the step S1 culture medium of microorganism species first, in 28-32
Shaken cultivation 20-24 hours at DEG C, so as to obtain the first microflora degradation liquid.
4. the preparation method of the microbial degradation liquid of oily sludge according to claim 1, it is characterised in that in the step
In rapid S2, the complex microbial community of gram meter is cultivated with the microorganism species first based on volume milliliter by weight
The ratio of base is 1:3000-4000.
5. the preparation method of the microbial degradation liquid of oily sludge according to claim 1, it is characterised in that the step
Rapid S3 specifically includes following steps： S3-1：1.5 g beef are added in the mixed liquor I obtained to step S1-1
Medicinal extract and 5 g peptones, are sufficiently stirred for, obtain mixed liquor I I； S3-2：Into the mixed liquor I I, trace element is added
The volume ratio of the aqueous solution, the mixed liquor I I and the trace element water-soluble liquid is 20:1, it is then sufficiently mixed, so as to obtain
The culture medium of microorganism species second；The trace element water-soluble liquid is 0.1g sodium molybdates, 0.08g aluminum nitrates, 0.04g Portugals
Grape saccharic acid zinc, 0.02g zinc chloride, 0.07g copper sulphate, 0.01g boric acid, 0.12g magnesium nitrates, 0.02g copper chlorides, 0.05g chlorinations
Cobalt, 0.03g manganese chlorides, 0.08g ferrous sulfate, 0.02g stannic chlorides and 0.06g potassium chloride be dissolved in 1000 ml distilled water and
Obtain.
6. the preparation method of the microbial degradation liquid of the oily sludge according to claim any one of 1-5, its feature exists
In the step S4 is specially：At room temperature, according to 1:40-46 volume ratio, the first microflora degradation liquid is added to
In second culture medium, and 38-40 DEG C is warming up to 1 DEG C/min of heating rate under agitation, shaken at such a temperature
Culture 5-6 hours is swung, then 48 ± 1 DEG C are warming up to again with 0.4-0.6 DEG C/min of heating rate, and train at such a temperature
Support 100-120 minutes, so as to obtain the second microflora degradation liquid i.e. microbial degradation liquid of oily sludge.
7. the degradation process method of the microbial degradation liquid for the oily sludge that any preparation methods of claim 1-6 are obtained,
Characterized in that, the second microflora degradation liquid is added into progress microbial degradation processing in oily sludge.
8. the degradation process method of microbial degradation liquid according to claim 7, it is characterised in that comprise the following steps：
S5：Using the second microflora degradation liquid come oily sludge of degrading, microbial degradation processing is completed；It is specific as follows：
S5-1：The second microflora degradation liquid is diluted to 10-15 times with deionized water, microflora degradation dilution is obtained；
S5-2：The microflora degradation dilution is added in oily sludge, at 40-50 DEG C of temperature, the 10- that degrades is sufficiently stirred for
20 days, so as to complete the microbial degradation processing of oily sludge.
9. the biodegradation process for treating according to claim 8, it is characterised in that：It is described in the step S5-2
Composite assistant is also added when microflora degradation dilution is added in oily sludge simultaneously, the composite assistant is mass ratio 1:8-
10 algae albumen glycolipid and the mixture of neopelex.
10. the biodegradation process for treating according to claim 9, it is characterised in that：In the step S5-2,
The microflora degradation dilution and the ratio for the oily sludge counted and counted using quality gram using dry weight counted using volume milliliter is 100:
20-30；The mass ratio of the composite assistant and the oily sludge counted using dry weight is 1:80-120.
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