CN102464438A - Method for degrading oily sludge of well site by using microorganisms - Google Patents
Method for degrading oily sludge of well site by using microorganisms Download PDFInfo
- Publication number
- CN102464438A CN102464438A CN201010550531XA CN201010550531A CN102464438A CN 102464438 A CN102464438 A CN 102464438A CN 201010550531X A CN201010550531X A CN 201010550531XA CN 201010550531 A CN201010550531 A CN 201010550531A CN 102464438 A CN102464438 A CN 102464438A
- Authority
- CN
- China
- Prior art keywords
- oily sludge
- well site
- weight ratio
- days
- degrading
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fertilizers (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to 6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg/kg to 1257 mg/kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 days in the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.
Description
Technical field
The present invention relates to a kind of utilization and from the oily sludge of well site, oil field, loess tableland district, cultivate, screen the method that obtains microbiological deterioration well site oily sludge.
Background technology
Oil-containing greasy filth complicated component, intractability is big.At present, the oily sludge treatment technology that adopts both at home and abroad is broadly divided into three types: physical chemistry processing, biological treatment and comprehensive utilization technique.Wherein physical chemistry handle with comprehensive utilization technique because in various degree have limitation such as secondary pollution, complex process, processing cost height, ME difficulty are big, be difficult to drop into large-scale application.
Bioremediation is divided two types, and the one, in oily sludge, add nutritive substance, aeration promotes mud indigenous microorganism growing multiplication, thereby realizes the degraded to pollutent.The 2nd, in oily sludge, add the microbiobacterial agent of efficient degradation petroleum hydrocarbon.The advantage of biologic treating technique is: the one, and can not form secondary pollution or cause pollutent to shift; The 2nd, technology is simple, and processing costs is low; The 3rd, treatment effect is good, and through biochemical treatment, the pollutent residual quantity can reduce significantly.Research shows, adds petroleum hydrocarbon degradation bacterium degradation rate is brought up to more than 50%.Utilize metabolism tensio-active agent mikrobe that oily sludge is handled; Improve petroleum hydrocarbon solubleness; Can improve the degradation efficiency of petroleum hydrocarbon, utilize biological treatment can remove PAHs class objectionable impurities, biological treatment can degrade simultaneously aliphatic hydrocarbon and aromatic hydrocarbon in the oily sludge; But there is limitation such as receiving such environmental effects is big, long processing period in bioremediation, and the treatment technology of the oily sludge of therefore carrying out a biological disposal upon does not also drop into mass-producing at home and uses.The efficient flora of research and establishment, optimal control condition, shortening the processing cycle is the main contents of this method research at present.
Summary of the invention
The purpose of this invention is to provide one group of bacterial classification that different components compound in the crude oil is had the efficient degradation ability; Construct the microorganism species that well site, oil field, loess tableland district oily sludge is had efficient repair, be used to solve well site lower concentration oil-containing mud pollution problems under the dispersion of well site, oil field, loess tableland district, the ungetable physical environment.
A kind of method of utilizing microbiological deterioration well site oily sludge of the present invention, concrete scheme is following:
1, efficient degrading bacteria group forms
From the oily sludge of well site, oil field, loess tableland district through cultivation, separation, screening, domestication, mutagenesis; Obtain Pseudomonas aeruginosa, micrococcus luteus, subtilis, 4 kinds of degradation bacteria of Acinetobacter junii, by weight 1: 1: 1: 1 additive capacity mixes to be formed;
2, the degradation condition that suits
The suitable degradation condition of mixing microorganisms flora is original ph=6, and carbon weight nitroxide ratio equals 100: 3, and the carbon phosphorus weight ratio equals 100: 0.6, and 30 ℃ of temperature, other condition do not have strict restriction, are provided by loess tableland district natural condition.
3, implementation step
(1) greasy filth that the well site ground crude oil is polluted moves to the well site oily sludge and handles prefabricated bed bioreactor, pulverizes smooth, the irrigation in back, requires to add organic nitrogenous fertilizer, phosphate fertilizer according to degradation condition;
(2) with after the mixing microorganisms flora dilution for preparing, initial effective concentration is added in the oily sludge by 5pp, and executing the bacterium cyclomorphosis is 20 days, and in totally three processing cycles, the concentration that bacterium is executed in second and third time is 3ppm;
(3) field management utilizes reactor drum control system for handling temperature at 30 ℃, execute bacterium and fertilizer for the first time after, whenever added nitrogenous fertilizer, phosphate fertilizer in proportion at a distance from 15 days, whenever digged, ventilate to reactor drum at a distance from 5 days, water, finish to the entire treatment stage.
The high effective microbial community that the present invention constructs can reduce the content of the petroleum substance in the well site oily sludge (soil) significantly; Save a large amount of transportations, equipment loss and maintenance cost; Do not cause secondary pollution, be particularly useful under the dispersion of well site, oil field, loess tableland district, ungetable physical environment, solving well site lower concentration oil-containing mud pollution problems.
Embodiment
Embodiment 1
Synergistic effect to mikrobe is studied; Carry out oily sludge degradation capability analysis and research research according to the synergistic effect of different strain; Is 1: 1: 1 with the Pseudomonas aeruginosa that obtains, micrococcus luteus, subtilis, Acinetobacter junii according to adding weight: 1 mixed; In the laboratory, in the 72h, the mass concentration that adds the system for handling PetroChina Company Limited. class of mixed bacterium is reduced to 1257mg/kg from 17214mg/kg; Degradation rate is 92.7%, has improved 57.3% than the oil degradation rate of control group.
Embodiment 2
To on-the-spot oil length is that 10.55% mud carries out 56 days Processing Test
1, efficient degrading bacteria group forms
From the oily sludge of well site, oil field, loess tableland district through cultivation, separation, screening, domestication, mutagenesis; Obtain Pseudomonas aeruginosa, micrococcus luteus, subtilis, 4 kinds of degradation bacteria of Acinetobacter junii, by weight 1: 1: 1: 1 additive capacity mixes to be formed;
2, the degradation condition that suits
The suitable degradation condition of mixing microorganisms flora is original ph=6, and carbon weight nitroxide ratio equals 100: 3, and the carbon phosphorus weight ratio equals 100: 0.6, and 30 ℃ of temperature, other condition do not have strict restriction, are provided by loess tableland district natural condition.
3, implementation step
(1) greasy filth that the well site ground crude oil is polluted moves to the well site oily sludge and handles prefabricated bed bioreactor, pulverizes smooth, the irrigation in back, requires to add organic nitrogenous fertilizer, phosphate fertilizer according to degradation condition;
(2) with after the mixing microorganisms flora dilution for preparing, initial effective concentration is added in the oily sludge by 5pp, and executing the bacterium cyclomorphosis is 20 days, and in totally three processing cycles, the concentration that bacterium is executed in second and third time is 3ppm;
(3) field management utilizes reactor drum control system for handling temperature at 30 ℃, apply microbial inoculum and fertilizer for the first time after, whenever added nitrogenous fertilizer, phosphate fertilizer in proportion at a distance from 15 days, whenever digged, ventilate to reactor drum at a distance from 5 days, water, finish to the entire treatment stage.
Degradation bacteria is 89.1% to the clearance of oil.
Claims (1)
1. method of utilizing microbiological deterioration well site oily sludge is characterized in that:
(1) from the oily sludge of well site, oil field, loess tableland district through cultivation, separation, screening, domestication, mutagenesis; Obtaining Pseudomonas aeruginosa, micrococcus luteus, subtilis, 4 kinds of degradation bacteria of Acinetobacter junii, is 1: 1: 1 according to weight ratio: 1 is mixed into oily sludge degraded microorganism species;
(2) degradation condition that suits
The suitable degradation condition of microorganism species is original ph=6, and carbon weight nitroxide ratio equals 100: 3, and the carbon phosphorus weight ratio equals 100: 0.6, and 30 ℃ of temperature, other condition do not have strict restriction, are provided by loess tableland district natural condition;
(3) implementation step
1) greasy filth that the well site ground crude oil is polluted moves to the well site oily sludge and handles prefabricated bed bioreactor, pulverizes smooth, the irrigation in back, requires to add organic nitrogenous fertilizer, phosphate fertilizer according to degradation condition;
After the mixing degraded microorganism species dilution that 2) will prepare, initial effective concentration is added in the oily sludge by 5pp, and executing the bacterium cycle is 20 days, and in totally three processing cycles, bacterium is executed in second and third time, and concentration is 3ppm;
3) field management utilizes reactor drum control system for handling temperature at 30 ℃, execute bacterium and fertilizer for the first time after, whenever added nitrogenous fertilizer, phosphate fertilizer in proportion at a distance from 15 days, whenever digged, ventilate to reactor drum at a distance from 5 days, water, finish to the entire treatment stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010550531 CN102464438B (en) | 2010-11-18 | 2010-11-18 | Method for degrading oily sludge of well site by using microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010550531 CN102464438B (en) | 2010-11-18 | 2010-11-18 | Method for degrading oily sludge of well site by using microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102464438A true CN102464438A (en) | 2012-05-23 |
| CN102464438B CN102464438B (en) | 2013-07-31 |
Family
ID=46068605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010550531 Active CN102464438B (en) | 2010-11-18 | 2010-11-18 | Method for degrading oily sludge of well site by using microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102464438B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674648A (en) * | 2012-06-01 | 2012-09-19 | 中国石油天然气股份有限公司 | Method for treating oily sludge |
| CN102747023A (en) * | 2012-07-27 | 2012-10-24 | 长安大学 | Micrococcus luteus and application thereof |
| CN103275910A (en) * | 2013-06-27 | 2013-09-04 | 南开大学 | Method of degrading anthracene in polycyclic aromatic hydrocarbons with saline-alkali tolerant micrococcusluteus TJTB70 |
| CN103787508A (en) * | 2014-01-16 | 2014-05-14 | 兰州石化职业技术学院 | Application of microbial compound agent in treatment of petrochemical sewage and sludge |
| CN104140962A (en) * | 2013-10-29 | 2014-11-12 | 中国石油化工集团公司 | Immobilization method for petroleum degrading bacterial group |
| CN104450583A (en) * | 2014-12-12 | 2015-03-25 | 黑龙江省科学院大庆分院 | Preparation method of petroleum-degrading acinetobacter junii bacillus |
| CN104803721A (en) * | 2014-01-27 | 2015-07-29 | 中国石油天然气股份有限公司 | Biological composting processing method of oil-based drilling wastes |
| CN106565066A (en) * | 2016-11-10 | 2017-04-19 | 湖北亦胜环保生物技术有限公司 | Harmless biological comprehensive treatment method for oily sludge |
| CN107299066A (en) * | 2017-06-21 | 2017-10-27 | 中国石油化工股份有限公司 | The preparation method and degradation process method of a kind of microbial degradation liquid of oily sludge |
| CN109020126A (en) * | 2018-07-13 | 2018-12-18 | 胜利油田森诺胜利工程有限公司 | A kind of construction technology for bioremediation of oily sludge |
| CN109158093A (en) * | 2018-10-16 | 2019-01-08 | 西北师范大学 | A kind of preparation that loess loads pvdf membrane and the application in water remediation |
| CN109420671A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420675A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420677A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420672A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN110668665A (en) * | 2019-08-26 | 2020-01-10 | 西安建筑科技大学 | Preparation method of excess sludge treating agent |
| CN110713947A (en) * | 2019-10-30 | 2020-01-21 | 中国石油集团川庆钻探工程有限公司长庆井下技术作业公司 | Compound microbial agent for repairing petroleum pollution and preparation method thereof |
| CN115724563A (en) * | 2021-08-31 | 2023-03-03 | 中国石油天然气股份有限公司 | Mobile aerobic microorganism oil sludge treatment system and treatment method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| EP1978099A1 (en) * | 2005-11-04 | 2008-10-08 | AIM Asset Management Co., Ltd. | Antibacterial substance dm0507 and utilization of the same |
-
2010
- 2010-11-18 CN CN 201010550531 patent/CN102464438B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| EP1978099A1 (en) * | 2005-11-04 | 2008-10-08 | AIM Asset Management Co., Ltd. | Antibacterial substance dm0507 and utilization of the same |
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674648B (en) * | 2012-06-01 | 2013-09-04 | 中国石油天然气股份有限公司 | Method for treating oily sludge |
| CN102674648A (en) * | 2012-06-01 | 2012-09-19 | 中国石油天然气股份有限公司 | Method for treating oily sludge |
| CN102747023A (en) * | 2012-07-27 | 2012-10-24 | 长安大学 | Micrococcus luteus and application thereof |
| CN103275910B (en) * | 2013-06-27 | 2015-03-25 | 南开大学 | Method of degrading anthracene in polycyclic aromatic hydrocarbons with saline-alkali tolerant micrococcusluteus TJTB70 |
| CN103275910A (en) * | 2013-06-27 | 2013-09-04 | 南开大学 | Method of degrading anthracene in polycyclic aromatic hydrocarbons with saline-alkali tolerant micrococcusluteus TJTB70 |
| CN104140962A (en) * | 2013-10-29 | 2014-11-12 | 中国石油化工集团公司 | Immobilization method for petroleum degrading bacterial group |
| CN103787508A (en) * | 2014-01-16 | 2014-05-14 | 兰州石化职业技术学院 | Application of microbial compound agent in treatment of petrochemical sewage and sludge |
| CN104803721A (en) * | 2014-01-27 | 2015-07-29 | 中国石油天然气股份有限公司 | Biological composting processing method of oil-based drilling wastes |
| CN104450583A (en) * | 2014-12-12 | 2015-03-25 | 黑龙江省科学院大庆分院 | Preparation method of petroleum-degrading acinetobacter junii bacillus |
| CN106565066A (en) * | 2016-11-10 | 2017-04-19 | 湖北亦胜环保生物技术有限公司 | Harmless biological comprehensive treatment method for oily sludge |
| CN107299066A (en) * | 2017-06-21 | 2017-10-27 | 中国石油化工股份有限公司 | The preparation method and degradation process method of a kind of microbial degradation liquid of oily sludge |
| CN107299066B (en) * | 2017-06-21 | 2020-08-28 | 中国石油化工股份有限公司 | Preparation method and degradation treatment method of microbial degradation liquid containing oily sludge |
| CN109420671A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420675A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420677A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420672A (en) * | 2017-08-29 | 2019-03-05 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN109420675B (en) * | 2017-08-29 | 2021-12-21 | 中国石油化工股份有限公司 | Soil remediation composition and application and soil bioremediation method |
| CN109020126A (en) * | 2018-07-13 | 2018-12-18 | 胜利油田森诺胜利工程有限公司 | A kind of construction technology for bioremediation of oily sludge |
| CN109020126B (en) * | 2018-07-13 | 2021-09-07 | 山东宇尚节能环保科技有限公司 | Construction process for bioremediation of oily sludge |
| CN109158093A (en) * | 2018-10-16 | 2019-01-08 | 西北师范大学 | A kind of preparation that loess loads pvdf membrane and the application in water remediation |
| CN110668665A (en) * | 2019-08-26 | 2020-01-10 | 西安建筑科技大学 | Preparation method of excess sludge treating agent |
| CN110713947A (en) * | 2019-10-30 | 2020-01-21 | 中国石油集团川庆钻探工程有限公司长庆井下技术作业公司 | Compound microbial agent for repairing petroleum pollution and preparation method thereof |
| CN115724563A (en) * | 2021-08-31 | 2023-03-03 | 中国石油天然气股份有限公司 | Mobile aerobic microorganism oil sludge treatment system and treatment method |
| CN115724563B (en) * | 2021-08-31 | 2024-06-14 | 中国石油天然气股份有限公司 | Mobile aerobic microorganism sludge treatment system and treatment method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102464438B (en) | 2013-07-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102464438B (en) | Method for degrading oily sludge of well site by using microorganisms | |
| Bohutskyi et al. | Effects of inoculum size, light intensity, and dose of anaerobic digestion centrate on growth and productivity of Chlorella and Scenedesmus microalgae and their poly-culture in primary and secondary wastewater | |
| Gentili | Microalgal biomass and lipid production in mixed municipal, dairy, pulp and paper wastewater together with added flue gases | |
| Zhang et al. | Aeration-induced CO2 stripping, instead of high dissolved oxygen, have a negative impact on algae–bacteria symbiosis (ABS) system stability and wastewater treatment efficiency | |
| Gao et al. | Performance of piggery wastewater treatment and biogas upgrading by three microalgal cultivation technologies under different initial COD concentration | |
| Yu et al. | Coupling of biochar-mediated absorption and algal-bacterial system to enhance nutrients recovery from swine wastewater | |
| Wang et al. | Organic degrading bacteria and nitrifying bacteria stimulate the nutrient removal and biomass accumulation in microalgae-based system from piggery digestate | |
| Xu et al. | Effects of influent C/N ratios and treatment technologies on integral biogas upgrading and pollutants removal from synthetic domestic sewage | |
| CN101597576B (en) | Solid microbial agent to remedy soil contaminated by petroleum, preparation method thereof and application | |
| CN105174476B (en) | Activated sludge and microalgae coupling particle system and its structure and operation method for wastewater treatment | |
| Qi et al. | Enhancing fermentation wastewater treatment by co-culture of microalgae with volatile fatty acid-and alcohol-degrading bacteria | |
| CN103159529B (en) | Technical method for biological fermentation of liquid dung and biogas slurry | |
| Lv et al. | Microbial community composition and function in a pilot-scale anaerobic-anoxic-aerobic combined process for the treatment of traditional Chinese medicine wastewater | |
| Meng et al. | Assessment of nutrient removal and microbial population dynamics in a non-aerated vertical baffled flow constructed wetland for contaminated water treatment with composite biochar addition | |
| CN103084377B (en) | The process of changing food waste and recycling | |
| Koutra et al. | Microalgal post-treatment of anaerobically digested agro-industrial wastes for nutrient removal and lipids production | |
| CN106277366B (en) | A kind of pig-breeding wastewater treatment microbial microbial inoculum and preparation method and application | |
| Wicker et al. | Application of Nordic microalgal-bacterial consortia for nutrient removal from wastewater | |
| CN109482638A (en) | A kind of pseudomonas drop oil bacterium L-1 and its microorganism mix bacterium agent and oil-polluted soils in-situ remediation method | |
| CN101186889A (en) | Thermophilic microorganism preparation for treating sewage and sludge, preparation method and application thereof | |
| Liu et al. | Strengthen effects of dominant strains on aerobic digestion and stabilization of the residual sludge | |
| Chen et al. | Enhancing biodegradation of wastewater by microbial consortia with fractional factorial design | |
| Wu et al. | Temperature modulating sand-consolidating cyanobacterial biomass, nutrients removal and bacterial community dynamics in municipal wastewater | |
| CN1718721A (en) | Method of treating oil contaminated soil and its special bacterin group | |
| CN107299066A (en) | The preparation method and degradation process method of a kind of microbial degradation liquid of oily sludge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |