CN109020126B - Construction process for bioremediation of oily sludge - Google Patents
Construction process for bioremediation of oily sludge Download PDFInfo
- Publication number
- CN109020126B CN109020126B CN201810770268.1A CN201810770268A CN109020126B CN 109020126 B CN109020126 B CN 109020126B CN 201810770268 A CN201810770268 A CN 201810770268A CN 109020126 B CN109020126 B CN 109020126B
- Authority
- CN
- China
- Prior art keywords
- soil
- oil
- dominant
- bacteria
- sludge sand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Processing Of Solid Wastes (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention provides a construction process for bioremediation of oily sludge, which comprises the following process steps: 1) taking an oil sludge sand sample, carrying out experimental analysis, selecting matched engineering bacteria, and adding acid, alkali and heavy metal elements contained in the oil sludge sand in the cultivation process to enable flora capable of adapting to or generating variation to survive to become dominant flora; 2) meanwhile, selecting nutrients; 3) aiming at oil sludge sand with different oil contents, mixing, crushing and maintaining the oil sludge sand added with the nutrient and the dominant microbial inoculum, and controlling the pH, EC, oxygen content and humidity of soil through a soil detector; 4) detecting the average number of dominant bacteria in soil once every 10 days in a laboratory, and supplementing dominant bacteria according to the activity and magnitude order of the dominant bacteria; 5) according to the repairing progress, sending a detection mechanism to detect the oil content of the soil about once every 15 days; has the advantages that: environment-friendly, no secondary pollution, low treatment price, simple and easy operation, high treatment efficiency and the like.
Description
Technical Field
The invention relates to the field of soil environmental protection, in particular to a process for resource comprehensive utilization and pollution control of oily sludge.
Background
The oil-containing silt is a mixture of oil and silt generated in the processes of oil and gas exploration, exploitation, gathering and transportation and wastewater (liquid) treatment, and oil-containing debris generated by using oil-based mud in the process of drilling. According to the characteristics of solid wastes of petroleum enterprises, the oil sludge is not only a waste in production, but also a valuable secondary resource.
According to the 5.2.1 th item in the technical requirements of comprehensive utilization of resources of oily sludge in onshore oil and gas exploitation and pollution control in the current industry standard, the total amount of petroleum hydrocarbon in the residual solid phase after the oily sludge is treated is required to be not more than 2%, and the residual solid phase after treatment is easy to be used for laying a drift well and paving well site base materials. The conventional physical and chemical treatment method for the oily sludge comprises the following steps: solvent extraction, incineration, coking, oil-containing sludge profile control, and the like. Most of the existing methods have high treatment cost, cannot meet the requirement on treatment effect and are easy to generate secondary pollution.
The biological treatment method (bioremediation) is a method for decomposing toxic substances into C02 and H2O by stimulating or adding hydrocarbon degradation microorganisms, has the advantages of environmental friendliness, no secondary pollution, low treatment price and the like, and is considered to be the most promising method for treating petroleum pollution at present.
At present, the technology is mature in the application of oil-containing sludge treatment in oil fields, the treatment effect completely meets the industrial standard through detection of a plurality of detection mechanisms such as Lu-loop academy of sciences, and the popularization and the application of the technology in the oil-containing sludge treatment in oil fields can be realized.
Disclosure of Invention
The invention aims to provide a construction process for bioremediation of oily sludge, which avoids the defects of the prior art.
The technical scheme of the invention is as follows:
a construction process for bioremediation of oily sludge comprises the following process steps:
1) taking an oil sludge sand sample, carrying out experimental analysis, selecting matched engineering bacteria from a bacteria seed bank, adding acid, alkali and heavy metal elements contained in the oil sludge sand in the cultivation process, and simulating the growth inhibition and toxic action of pollutants on the bacteria, so that part of flora capable of adapting to or generating variation can survive to become dominant flora;
2) meanwhile, nutrients are selected, wherein the mixture ratio of the substances is 35% of humic acid, 11.2% of nitrogen, 2.1% of phosphorus, 3.2% of potassium, 1.34% of magnesium, 0.04% of zinc, 0.027% of manganese and 0.035% of copper;
3) aiming at oil sludge sand with different oil contents, 2% of nutrient and 1% of dominant microbial inoculum are added on site with the oil content below 8%, 2.5% of nutrient and 2% of dominant microbial inoculum are added with the oil content above 8%, the oil sludge sand added with the nutrient and the dominant microbial inoculum is mixed, crushed and maintained by a rotary cultivator, and the PH, EC and oxygen content and humidity of soil are controlled by a multifunctional digital soil detector;
4) detecting the average number of the dominant bacteria in the soil once by using a blood counting plate in a laboratory every 10 days, supplementing and adding the dominant bacteria according to the activity and the magnitude order of the dominant bacteria, and if the number of the bacteria in the soil is less than 3.75 × 107/kg, supplementing the dominant bacteria;
5) and sending a detection mechanism with CMA certification to detect the oil content of the soil once about every 15 days according to the repair progress until the detection result is less than three thousandths.
The engineering bacteria in the step 1) are Korvilus, Oilspirochete, Neptunomonas and the like.
The concrete numerical values of the soil in the step 3) are as follows: the temperature of the soil is between 15 and 45 ℃, the PH of the soil is between 5 and 8, the EC of the soil is between 0.4 and 5, the oxygen content of the soil is more than or equal to 15 percent, and the relative humidity is more than or equal to 70 percent.
Compared with the prior art, the method has the advantages of environmental friendliness, no secondary pollution, low treatment price, simple and easy operation, high treatment efficiency and the like.
Drawings
FIG. 1 is a process flow diagram of one embodiment of the present invention.
Detailed Description
A construction process for bioremediation of oily sludge comprises the following process steps:
1) taking an oil sludge sand sample, carrying out experimental analysis, selecting matched engineering bacteria from a bacteria seed bank, adding acid, alkali and heavy metal elements contained in the oil sludge sand in the cultivation process, and simulating the growth inhibition and toxic action of pollutants on the bacteria, so that part of flora capable of adapting to or generating variation can survive to become dominant flora;
2) meanwhile, nutrients are selected, wherein the mixture ratio of the substances is 35% of humic acid, 11.2% of nitrogen, 2.1% of phosphorus, 3.2% of potassium, 1.34% of magnesium, 0.04% of zinc, 0.027% of manganese and 0.035% of copper;
3) aiming at oil sludge sand with different oil contents, 2% of nutrient and 1% of dominant microbial inoculum are added on site with the oil content below 8%, 2.5% of nutrient and 2% of dominant microbial inoculum are added with the oil content above 8%, the oil sludge sand added with the nutrient and the dominant microbial inoculum is mixed, crushed and maintained by a rotary cultivator, and the PH, EC and oxygen content and humidity of soil are controlled by a multifunctional digital soil detector;
4) detecting the average number of the dominant bacteria in the soil once by using a blood counting plate in a laboratory every 10 days, supplementing and adding the dominant bacteria according to the activity and the magnitude order of the dominant bacteria, and if the number of the bacteria in the soil is less than 3.75 × 107/kg, supplementing the dominant bacteria;
5) and sending a detection mechanism with CMA certification to detect the oil content of the soil once about every 15 days according to the repair progress until the detection result is less than three thousandths.
The engineering bacteria in the step 1) are Korvilus, Oilspirochete, Neptunomonas and the like.
The concrete numerical values of the soil in the step 3) are as follows: the temperature of the soil is between 15 and 45 ℃, the PH of the soil is between 5 and 8, the EC of the soil is between 0.4 and 5, the oxygen content of the soil is more than or equal to 15 percent, and the relative humidity is more than or equal to 70 percent.
The first embodiment is as follows:
oily sludge treatment test project for certain united station
Sampling on site, and detecting the oil content, the PH value and the heavy metal content in a laboratory. The results are as follows:
selecting strains of the genera Corsveriella and Oilusspira from a strain library, and simulating the strains in the culture process
Under the adverse conditions, the strain DK-017 with suitable conditions is finally cultured through continuous domestication for 10 days.
After the on-site oil sludge sand is spread and aired, 2.5 percent of nutrient and 2 percent of microbial inoculum are added, and the rotary cultivator is used for rotary tillage on the oil sludge sand twice a day.
On the 10 th day, the 20 th day and the 30 th day, on-site oil sludge sand samples are respectively taken for strain technical observation, and the measured DK-017 quantity is 4.51 x 107/kg、5.45*107/kg、5.86*107And/kg, does not need to supplement microbial inoculum.
Four-point sampling inspection is carried out before and after oil sludge sand treatment, and the oil content is as follows:
example II test project for oily sludge treatment of acidic wastewater tank of certain plant
Sampling on site, and detecting the oil content, the PH value and the heavy metal content in a laboratory. The results are as follows:
selecting oil spirillum strain from the strain bank, simulating the above unfavorable conditions in the culture process, and continuously domesticating for 14 days to finally culture the strain ZQ-021 with proper conditions.
After the on-site oil sludge sand is spread and aired, 2% of nutrient and 1% of microbial inoculum are added, and rotary tillage is carried out on the oil sludge sand twice a day by using a rotary cultivator.
On the 10 th day, the 20 th day and the 30 th day, on-site oil sludge sand samples are respectively taken for strain technical observation, and the measured DK-017 quantity is 2.81 x 107/kg、4.75*107/kg、5.21*107And/kg, supplementing the microbial inoculum once.
Four-point sampling inspection is carried out before and after oil sludge sand treatment, and the oil content is as follows:
| 1 | 2 | 3 | 4 | |
| before treatment | 6.58% | 6.12% | 5.27% | 4.31% |
| 15 days intermediate sampling | 1.24% | 1.08% | 2.84% | 1.91% |
| After treatment | 0.17% | 0.28% | 0.22% | 0.14% |
Claims (1)
1. A construction process for bioremediation of oily sludge is characterized by comprising the following process steps:
1) taking an oil sludge sand sample, carrying out experimental analysis, selecting matched engineering bacteria from a bacteria seed bank, adding acid, alkali and heavy metal elements contained in the oil sludge sand in the cultivation process, and simulating the growth inhibition and toxic action of pollutants on the bacteria, so that part of flora capable of adapting to or generating variation can survive to become dominant flora;
2) meanwhile, nutrients are selected, wherein the mixture ratio of the substances is 35% of humic acid, 11.2% of nitrogen, 2.1% of phosphorus, 3.2% of potassium, 1.34% of magnesium, 0.04% of zinc, 0.027% of manganese and 0.035% of copper;
3) aiming at oil sludge sand with different oil contents, 2% of nutrient and 1% of dominant microbial inoculum are added on site with the oil content below 8%, 2.5% of nutrient and 2% of dominant microbial inoculum are added with the oil content above 8%, the oil sludge sand added with the nutrient and the dominant microbial inoculum is mixed, crushed and maintained by a rotary cultivator, and the pH, EC and oxygen content and humidity of soil are controlled by a multifunctional digital soil detector;
4) detecting the average number of the dominant bacteria in the soil once by using a blood counting plate in a laboratory every 10 days, supplementing and adding the dominant bacteria according to the activity and the magnitude order of the dominant bacteria, and if the number of the bacteria in the soil is less than 3.75 x 107Kg, then need to supplement;
5) sending a detection mechanism with CMA certification to detect the oil content of the soil once every 15 days according to the repair progress until the detection result is less than three thousandths;
the engineering bacteria in the step 1) are Corsvervia, Oilspira and Neptunomonas; the concrete numerical values of the soil in the step 3) are that the temperature of the soil is between 15 and 45 ℃, the pH of the soil is between 5 and 8, the EC of the soil is between 0.4 and 5, the oxygen content of the soil is more than or equal to 15 percent, and the relative humidity is more than or equal to 70 percent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810770268.1A CN109020126B (en) | 2018-07-13 | 2018-07-13 | Construction process for bioremediation of oily sludge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810770268.1A CN109020126B (en) | 2018-07-13 | 2018-07-13 | Construction process for bioremediation of oily sludge |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109020126A CN109020126A (en) | 2018-12-18 |
| CN109020126B true CN109020126B (en) | 2021-09-07 |
Family
ID=64642146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810770268.1A Active CN109020126B (en) | 2018-07-13 | 2018-07-13 | Construction process for bioremediation of oily sludge |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109020126B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110078223A (en) * | 2019-04-30 | 2019-08-02 | 中国石油集团川庆钻探工程有限公司 | A kind of drilling well solid waste micro-organism treatment process |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003276537A8 (en) * | 2002-10-08 | 2004-05-04 | Econova Ab | Process for conversion of biomass |
| CN1803680A (en) * | 2005-12-20 | 2006-07-19 | 王鸿飞 | Apparatus and method for reclaiming, purifying and reutilizing boring mud |
| CN102108000A (en) * | 2011-01-12 | 2011-06-29 | 中国海洋石油总公司 | Biological treatment method of oilfield drilling waste |
| CN102464438A (en) * | 2010-11-18 | 2012-05-23 | 中国石油天然气股份有限公司 | Method for degrading oily sludge of well site by using microorganisms |
| CN103435241A (en) * | 2013-09-18 | 2013-12-11 | 中国石油集团川庆钻探工程有限公司 | Treatment process for drilling waste sludge in oil and gas field |
| CN104086065A (en) * | 2014-06-18 | 2014-10-08 | 中国石油集团川庆钻探工程有限公司 | Microbiological treatment technology for waste mud generated in drilling operation of petroleum and natural gas |
| JP5767854B2 (en) * | 2011-05-16 | 2015-08-19 | 日鉄住金環境株式会社 | Organic wastewater treatment method |
| CN105540994A (en) * | 2015-12-01 | 2016-05-04 | 内蒙古恒盛环保科技工程有限公司 | Resource utilization technology of drilling well wastes |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5853450A (en) * | 1995-09-22 | 1998-12-29 | Medical College Of Ohio | Method for treating bioorganic and wastewater sludges |
| CN101402504A (en) * | 2008-11-24 | 2009-04-08 | 李波 | Treatment system for wastewater without sewage sludge |
| MX2012004676A (en) * | 2009-10-22 | 2012-10-05 | Univ Illinois | Hydrothermal processing (htp) of algae grown in htp waste streams. |
| CN103449695B (en) * | 2012-06-04 | 2014-12-17 | 江苏博大环保股份有限公司 | Microbiological oil sludge treatment process |
| JP2015146797A (en) * | 2014-02-04 | 2015-08-20 | 彬令 大木 | Raising seedling culture medium (bed soil) made of fully organic materials without sterilization using agricultural waste materials and excreta as main raw material, and manufacturing method of the same |
| US9328007B2 (en) * | 2014-02-27 | 2016-05-03 | Hong Kong Baptist University | Biogenic flocculant composition to enhance flocculation and dewaterability of chemically enhanced primary treatment sludge |
| WO2016167037A1 (en) * | 2015-04-13 | 2016-10-20 | 富士電機株式会社 | Method for treating wastewater, and activator for treating wastewater |
| CN106348456B (en) * | 2015-10-21 | 2019-03-08 | 广东中微环保生物科技有限公司 | Creek water pollution superior microorganism original position ecological restoring method |
| CN106799131A (en) * | 2015-11-25 | 2017-06-06 | 无锡物语环境科技有限公司 | Complex microorganism deodorant with improvement denitrifying bacterium mentha pulegium essential oil spirulina |
| CN108048376B (en) * | 2018-02-06 | 2020-08-04 | 北京大学 | Degradation strain JN3 for petroleum hydrocarbons in oily sludge and application thereof |
-
2018
- 2018-07-13 CN CN201810770268.1A patent/CN109020126B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003276537A8 (en) * | 2002-10-08 | 2004-05-04 | Econova Ab | Process for conversion of biomass |
| CN1803680A (en) * | 2005-12-20 | 2006-07-19 | 王鸿飞 | Apparatus and method for reclaiming, purifying and reutilizing boring mud |
| CN102464438A (en) * | 2010-11-18 | 2012-05-23 | 中国石油天然气股份有限公司 | Method for degrading oily sludge of well site by using microorganisms |
| CN102108000A (en) * | 2011-01-12 | 2011-06-29 | 中国海洋石油总公司 | Biological treatment method of oilfield drilling waste |
| JP5767854B2 (en) * | 2011-05-16 | 2015-08-19 | 日鉄住金環境株式会社 | Organic wastewater treatment method |
| CN103435241A (en) * | 2013-09-18 | 2013-12-11 | 中国石油集团川庆钻探工程有限公司 | Treatment process for drilling waste sludge in oil and gas field |
| CN104086065A (en) * | 2014-06-18 | 2014-10-08 | 中国石油集团川庆钻探工程有限公司 | Microbiological treatment technology for waste mud generated in drilling operation of petroleum and natural gas |
| CN105540994A (en) * | 2015-12-01 | 2016-05-04 | 内蒙古恒盛环保科技工程有限公司 | Resource utilization technology of drilling well wastes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109020126A (en) | 2018-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gomez et al. | Optimization of field scale biopiles for bioremediation of petroleum hydrocarbon contaminated soil at low temperature conditions by response surface methodology (RSM) | |
| Zhang et al. | Characterizing microbial communities dedicated for conversion of coal to methane in situ and ex situ | |
| Camenzuli et al. | On-site and in situ remediation technologies applicable to petroleum hydrocarbon contaminated sites in the Antarctic and Arctic | |
| Antizar-Ladislao | Bioremediation: working with bacteria | |
| Banning et al. | Development of microbial diversity and functional potential in bauxite residue sand under rehabilitation | |
| CN105170644B (en) | A kind of biological renovation method of oil-polluted soils | |
| Ke et al. | Bioremediation of oily sludge by solid complex bacterial agent with a combined two-step process | |
| Dos Santos et al. | Exploratory analysis of the microbial community profile of the municipal solid waste leachate treatment system: A case study | |
| CN106045255A (en) | Microbiological treatment method of oily sludge | |
| Harkness et al. | Use of statistical tools to evaluate the reductive dechlorination of high levels of TCE in microcosm studies | |
| CN109020126B (en) | Construction process for bioremediation of oily sludge | |
| Du et al. | Bacteria and archaea involved in anaerobic mercury methylation and methane oxidation in anaerobic sulfate–rich reactors | |
| Dann et al. | Investigation and optimization of a passively operated compost-based system for remediation of acidic, highly iron-and sulfate-rich industrial waste water | |
| CN114480243A (en) | Microbial remediation method applied to non-foreign soil mining area | |
| CN104988087B (en) | Oil contaminated soil complex microorganism renovation agent and preparation method thereof | |
| Pereyra et al. | Effect of bioaugmentation and biostimulation on sulfate-reducing column startup captured by functional gene profiling | |
| Ujang et al. | Removal behaviour of residual pollutants from biologically treated palm oil mill effluent by Pennisetum purpureum in constructed wetland | |
| Sathish et al. | Biodegradation of leather solid waste and manipulation of methanogens and chromium-resistant microorganisms | |
| Steliga et al. | Optimisation research of petroleum hydrocarbon biodegradation in weathered drilling wastes from waste pits | |
| Li et al. | Bacterial communities fluctuate in abundance and diversity under simulated oil-contaminated seawater conditions | |
| Wang et al. | Activating soil nitrification by co-application of peanut straw biochar and organic fertilizer in a rare earth mining soil | |
| Dubey et al. | A study of the effect of red mud amendments on the growth of cyanobacterial species | |
| Okonofua et al. | Determination of suitable TPH remediation approach via MANOVA and inferential statistics assessment | |
| Tuo et al. | Decades of weathering improves coal waste biogeochemical properties by altering dissolved organic matter and microbial communities: A case‐study of a coal waste pile | |
| Uba et al. | Kinetics of biodegradation of total petroleum hydrocarbon in diesel contaminated soil as mediated by organic and inorganic nutrients |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20191216 Address after: 257000 No. 819, North Road, Dongying District, Shandong, Dongying Applicant after: Shandong Yu Yu energy-saving environmental protection technology Co., Ltd. Address before: 257000 Shandong Province, Dongying city the Yellow River Road No. 721 senuo Victory Mansion Applicant before: Shengli Oilfield Sino Shengli Engineering Co., Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |