CN1563342A - Microbe preparation in use for treating high difficult wastewater and preparation method - Google Patents
Microbe preparation in use for treating high difficult wastewater and preparation method Download PDFInfo
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- CN1563342A CN1563342A CN 200410005196 CN200410005196A CN1563342A CN 1563342 A CN1563342 A CN 1563342A CN 200410005196 CN200410005196 CN 200410005196 CN 200410005196 A CN200410005196 A CN 200410005196A CN 1563342 A CN1563342 A CN 1563342A
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Abstract
A microbiological preparation made from 105 types of acclimatized and integrated microorganisms belonging to 47 genuses can be used to process highly difficult waste waters of coking waste water, gourment powder waste water, antibiotic waste water, pigment waste water, paper making waste water and fluorating waste water.
Description
Technical field
The present invention relates to a kind of microbial preparation that is used for wastewater treatment and preparation method thereof.
Background technology
So-called highly difficult waste water is meant to contain the COD height, and BOD
5(biochemical oxygen demand (BOD)) is on the low side relatively, and contains the waste water of toxicant.Mainly be distributed in oil refining industry, petrochemical industry industry, dye well dyeing and finishing industry, pharmacy industry (particularly microbiotic production), agricultural chemicals industry, monosodium glutamate industry, coking industry, paper pulp production, wool textile industry, chemical industry production, rubber industry etc.Because toxic substance often has stronger restraining effect to microorganism, adopt ordinary activated sludge to handle highly difficult waste water and often be difficult to reach processing requirements, trace it to its cause mainly is that microbial activity in the mud is not high, to the every pollutent bad adaptability in the sewage, can't thoroughly decompose all contaminations.
Present stage, mainly there is following problem in the highly difficult waste water utilizing ordinary activated sludge to handle.
1. the biochemical treatment problem of difficult decomposing organic matter.
Usually the waste water that it is believed that BOD/COD＜0.3 is for being difficult to biochemistry.Prolong the residence time, changing the microbial growth condition is to receive some effects, but a large amount of power consumption meetings increases considerably production cost.It is also infeasible to add sanitary sewage, and one comes the collection of municipal effluent relatively more difficult, if two difficulty with dining room or other sewage introducing meeting increase processing.Thus, the processing of waste water such as coking chemical waste water, waste water from dyestuff, saccharin wastewater, nitrobenzene waste water, black liquid, PTA waste water is an a great problem.
2.SO
4 2-Inhibition to anaerobic system.
For highly difficult waste water, generally adopt the A-O method.Promptly earlier with after waste water acidifying, hydrolysis, the methanation, again through the aerobic treatment qualified discharge.Because SO
4 2-Existence, make SO in the anaerobic process
4 2-Reduction bacterium and methanobacteria competition nutrition, SO
4 2-Be converted into H
2S, H
2S has stronger toxic action to methanobacteria, and in anaerobic process, so the rate controlling step that is generated as anaerobic stages (bottleneck) of methane is finite concentration SO
4 2-Existence can make the removal of anaerobic stages BOD lose effect, thereby cause system deterioration, the water of releasing can't be up to standard.All can run into this situation in the wastewater treatment of citric acid, glutamate production enterprise, key is how at high SO
4 2-Handle well under the concentration, the balanced relation between the anaerobic bacteria flora, both allowed SO
4 2-The reduction bacteria growing does not become the dominant bacteria that suppresses methanobacteria again.
3.CL
-Influence to microorganism growth.
Because the requirement of manufacturing process can contain higher CL in some waste water
-, need carry out a large amount of dilutions as carrying out biochemical treatment, dilution can make investment and running cost all be doubled and redoubled in a large number, and has wasted water resources.Pharmacy waste water, saccharin wastewater, some waste water from dyestuff are all owing to high CL
-Content can't normally move the routine biochemistry treatment system.
4. contain the processing of ammonia nitrogen and phosphorus waste water.
Ammonia nitrogen and phosphorus are one of source of pollution in the water, are again the inhibitor of microorganism.The pollution in basin largely is the problem of eutrophication, removing ammonia nitrogen effectively can address this problem well, what domestic use was many is nitration denitrification technology, the removal of every kilogram of ammonia nitrogen of Theoretical Calculation need add alkali 3～4kg, also need to add carbon source in most technologies and carry out denitrification denitrogenation, cause running cost too high, or can't normally move.
In biochemical processing, the vigor of microorganism is the key of treatment technology and basic, so should solve adaptability and the capacity of decomposition of microorganism from start with at all.
Summary of the invention
What the present invention will solve is the technical problem that common sludge is difficult to handle highly difficult waste water, provides a kind of and can adapt to various highly difficult waste water, the microbial preparation of the various toxic substances of degrading well.
Further aim of the present invention provides a kind of preparation method of this microbial preparation.
The present invention adopts following technical scheme: be used to handle the microbial preparation (being designated hereinafter simply as the HSBEMBM preparation) of highly difficult waste water, contain following 105 kinds of microbial strainss from 47 genus:
Acetobacter aceti acetic acid acetobacter
The Acetobacter liquefaciens acetobacter that liquefies
Acetobacter xylinum acetobacter xylinum
The dry achromobacter of Achromobacter xerosis
Aeromonans hydrophila Aeromonas hydrophila
Aeromonas media Aeromonas media
Aeromonans sobria Aeromonas sobria
Bacillus alvei branch genus bacillus
Bacillus coagulans Bacillus coagulans
Bacillus subtilis subtilis
Bacillus leutis bacillus lentus
Bacillus firmus bacillus firmus
Bacillus mycoides shape genus bacillus
Bacillus megaterium bacillus megaterium
Bacillus alcalophilus Alkaliphilic bacillus
Bacillus cereus bacillus cereus
Bacillus licheniformis Bacillus licheniformis
Bacillus pumilus bacillus pumilus
Bacillus spaericus Bacillus sphaericus
Bacillus marinus bacillus marinus
Alcaligenes denitrificans denitrification subspecies
Alcaligenes faecalis Alcaligenes faecalis
Alcaligenes xylosoxydans Alcaligenes xylosoxidans
Brevibacterium acetylicum acetylene tyrothricin
Brevibacterium ammoniagenes separates the ammonia tyrothricin
Brevibacterium casei brevibacterium casei
The short genus bacillus of Brevibacillus brevis
Enterobacter cloacae enterobacter cloacae
Enterobacter aerogenes enteroaerogen
Enterobacter agglomerans enterobacter agglomerans
Thiobacillus?novellas
Thiobacillus?thioparus
Thiobacillus denitrificans denitrification thiobacillus
Thiobacillus thiooxidans thiobacillus thiooxidant
Thiorhodococcus minus sulphur rhodococcus
Rhodopseudomonas palustris Rhodopseudomonas palustris
Rhodopseudomonas acidphia rhodopseudomonas acidophilus
Gluconobacter albidus Qian Jingshi gluconobacter sp
Gluconobacte oxydans glucose oxidation and bacillus
Lactobacillus fermentum lactobacillus fermentum
Lactobacillus plantarum plant lactobacillus
Lactobacillus alimentarius digests Bacterium lacticum
Lactobacillus amylophillus food starch milk bacillus
Lactobacillus ruminis lactobacillus ruminis
Lactobacillus bervis short lactobacillus
Micrococcus leutus micrococcus luteus
Micrococcus halobius Micrococcus halobius
Pseudomonas alcaligenes Pseudomonas alcaligenes
Pseudomonas aureofaciens causes yellow pseudomonas
Pseudomonas chlororaphis Pseudomonas chlororaphis
The nitrated pseudomonas of Pseudomonas nitroreducens
Pseudomonas riboflavina riboflavin pseudomonas
Pseudomonas putina pseudomonas putida
Pseudomonas facilis Pseudomonas facilis
Paenibacillus gluconolyticus separates the glucan genus bacillus
Paenibacillus thiaminlyticus separates the VitB1 series bacillus
Saccharomyces?telluris
Beggiatoa?alba
Nitrobacter winogradskyi bacterium nitrobacter
Nitrosomonas europaea Nitrosomonas
Nitrosococcus?nitrosus
The narrow and small luminous bacillus of Photobacterium angustum
Photobacterium phosphoreum photobacterium phosphoreum
Photobacterium leiognathi abalone luminous bacillus
The richly endowed bacterium of Haloferax denitrificans denitrification salt
The richly endowed bacterium of Haloferax mediterranei Mediterranean Sea salt
Methanobacterium bryantii Bu Shi methagen
Methanobacterium paluster marsh methagen
Methanobacterium uliginosum mire methagen
Cellulomonas biazotes dinitrogen cellulomonas cartae
Cellulomonas fimi muck cellulomonas cartae
Kurthia zopfii Zuo Shi Al Kut Salmonella
Thiosphaera?pantotropha
Alcaligenes?sp
Chlorobium limicola mud is given birth to green bacterium
The long red bacterium of Erythrobacter longus
The red Zymomonas mobilis of Erothromonas ursincola
Azomonas macrocytogenes giant cell nitrogen Zymomonas mobilis
The yellow bacillus flavus of Xanthobacter flavus
Methylcoccus capsulatus pod membrane methyl coccus
Alteromonas denitrificans denitrification replaces Zymomonas mobilis
Alteromonas nigrifaciens produces black alternately Zymomonas mobilis
Telluria mxita mixes the refreshing bacterium in ground
Bacteroides cellulosovens Bacteroides cellulosolvens
Bacteroides stercoris Bacteroides stercoris
Many nutritive muds of Ilyobacter ploytropus bacillus
The false butyric acid vibrios of Pseudobutyrivibrio ruminis cud
Zymomonas mobilis is supported in Syntrophomonas wolfei Ao Shi syntrophism
The simple pimelobacter sp of Pimelobacter simplex
Pimelobacter tumescens swelling pimelobacter sp
The short shape bacillus of Brachybacterium faecium excrement
Jonesia denitrificans denitrification Jones Salmonella
The rotten rare bacillus of Rarobacter faecitabidus slag
Eubacterium formicigenerans Eubacterium formicigenerans
Eubacterium nitritogenes Eubacterium nitrotogenes
Eubacterium xylanophilum has a liking for the polyxylose Eubacterium
The aurantia small bacillus of Exiguobacterium aurantiacum
Bacillus is supported in Synteophobacter wolinii Wo Shi syntrophism
Bacillus is supported in Synteophobacter pnnigii Fen Shi syntrophism
Pelobacter acetylenicus acetylene occupies the mud bacillus
Pelobacter propionicus produces propionic acid and occupies the mud bacillus
Thiodictyon elegans U.S. Open Tennis sulphur bacterium
The purple capsule sulphur of Thiocystis violacea bacterium
Anaerovibrio glycerini glycerine anaerobism vibrios
Anaerovibrio lipolytica Anaerovibrio lipolytica
For reaching second purpose of the present invention; the technical scheme that is adopted is: after the process microbe to screen is handled with domestication in the laboratory with active sludge; bacterial classification after the screening is mixed; and contain above-mentioned 105 kinds of bacterial strains in the bacterial classification mixture after guaranteeing to be mixed; bacterial classification after being mixed adopts aerobic respectively and anaerobism is cultivated; bacterial classification after will cultivating is again cultivated bacterial classification by anaerobism: aerobic cultivation bacterial classification is 1: 3 a mixed; add stablizer and bacterial classification protective material, promptly get HSBEMBM preparation disclosed in this invention.
Wherein, three kinds of substratum are adopted in aerobic cultivation, be respectively bacterium perfect medium (as beef-protein medium), glucose perfect medium, cane sugar inorganic salt substratum, three kinds of substratum carry out inoculation culture respectively, culture condition is 30～32 ℃, and 120 rev/mins, the time is 24～36 hours, and adjust according to the growth situation in practice, cultivate and finish after 1: 1: 1 mixed is pressed in check.
Anaerobic culture medium adopts the glucose perfect medium, and culture temperature is 32～35 ℃, every two hours stirs 5 minutes, and incubation time is 48 hours.
Than general active sludge, the microbial preparation that is used to handle highly difficult waste water disclosed in this invention has the following advantages.
1. bacterial classification A wide selection of colours and designs, abundant amount can be formed different degradation biological chains at all contaminations, thoroughly pollutent is transformed and decomposes.
2. bacterial classification kind amount is enough to adapt to poisonous environment, and itself is nontoxic.
3. it is fast to remove pollution speed such as COD, SS, BOD, and soil removability is strong.
4. decomposing N H
3, thiomethyl alcohol, H
2S and organic acid ability are strong, so can deodorizing.
5. use microbial preparation of the present invention to handle highly difficult waste water, devices needed is simple, and is with low cost, and failure rate is low.
6. use microbial preparation of the present invention to handle highly difficult waste water, the mud generation is few, removes about 0.05 kilogram of per kilogram BOD excess sludge.
7. nitrogen removal efficiency is more than 85%, and tp removal rate is more than 90%, and the removal of sulphur rate is more than 60%.
8. sludge settling is good, the tightness height, and stability is high.
The microbial preparation that is used to handle highly difficult waste water disclosed in this invention can be used for Treatment of Wastewater in Coking, gourmet powder waste water, antibiotic waste water, waste water from dyestuff, black liquid and fluorine chemical waste water.In conjunction with different treatment process, can obtain the good treatment effect.
Description of drawings
Fig. 1 is the preparation flow figure that is used to handle the microbial preparation of highly difficult waste water disclosed in this invention.
Embodiment
Embodiment 1: preparation is used to handle the microbial preparation of highly difficult waste water
After the process microbe to screen is handled with domestication in the laboratory with active sludge, bacterial classification after the screening is mixed, wherein the anerobe combination cooperates with the ratio of aerobic bacteria combination in 1: 1, and contain 105 kinds of bacterial strains disclosed in this invention in the bacterial classification mixture after guaranteeing to be mixed, flora after will being mixed then carries out aerobic fermentation cultivation and anaerobically fermenting respectively and cultivates in two.
Three kinds of substratum are adopted in wherein aerobic cultivation, be respectively bacterium perfect medium (as beef-protein medium), glucose perfect medium, cane sugar inorganic salt substratum, three kinds of substratum carry out inoculation culture respectively, culture condition is 30 ℃, 120 rev/mins, time is 36 hours, cultivates and finishes after 1: 1: 1 mixed is pressed in check.
Wherein the component of beef-protein medium is: 0.5 part of extractum carnis, and 1.0 parts of peptones, 0.5 part in sodium-chlor, 100 parts in water, each component is mixed the back and is regulated pH to 7.0, sterilizes 20 minutes promptly for 121 ℃.
The component of glucose perfect medium is: 0.5 part of extractum carnis, and 1.0 parts of peptones, 1.0 parts of glucose, 100 parts in water, each component is mixed the back and is regulated pH to 7.0, sterilizes 20 minutes promptly for 121 ℃.
The component of cane sugar inorganic salt substratum is: 1.0 parts of glucose, 1.5 parts of sucrose, 0.02 part in calcium chloride, 0.05 part in sal epsom, 0.5 part of potassium primary phosphate, 0.5 part in ammonium sulfate, 0.02 part in sodium-chlor, 100 parts in water, each component mix the back and regulate pH to 7.0, sterilize 20 minutes promptly for 121 ℃.
Anaerobic culture medium adopts the glucose perfect medium, and culture temperature is 32 ℃, every two hours stirs 5 minutes, and incubation time is 48 hours.
With after the assay was approved through anaerobism cultivate and aerobic cultivation after flora be mixed with 1: 3 ratio, adding stablizer and bacterial classification protective material promptly get the microbial preparation that is used to handle highly difficult waste water disclosed in this invention after the assay was approved.The standard of check is exceeded to contain 105 kinds of bacterial classifications disclosed in this invention in the flora.
Embodiment 2: preparation is used to handle the microbial preparation of highly difficult waste water
Three kinds of medium pHs that aerobic cultivation is adopted are adjusted to 7.4,121 ℃ of sterilizations 15 minutes, and the culture condition of aerobic cultivation is 32 ℃, and 120 rev/mins, the time is 24 hours.The culture temperature that anaerobism is cultivated is 32 ℃, every two hours stirs 5 minutes, and incubation time is 48 hours.All the other preparation conditions and preparation process are with embodiment 1.
Embodiment 3: be used for Treatment of Wastewater in Coking
The coking chemical waste water component reaches tens of kinds more than, mainly is the heterogeneous ring compound of phenol compound, arene and polynuclear aromatics compounds of group, nitrogenous, oxygen or sulphur, is a kind of typical difficult organic trade effluent that decomposes.Application technology is an O-A-O technology during Treatment of Wastewater in Coking, promptly comprise pre-exposure system and two sections biochemical systems, the pre-exposure system is made up of a pre-exposure pond and a heavy pond, two sections biochemical systems are by anaerobism or oxygen compatibility pool, Aerobic Pond and second pond are formed, remove COD and large amount of organic by the pre-exposure pond, anaerobism or oxygen compatibility pool carry out denitrification denitrogenation, Aerobic Pond carries out nitrification, do not have additional carbon in the whole technological process, and each directly adds the HSBEMBM microbial preparation disclosed in this invention of volume 1.5%～4.0% separately and the gac of volume 1.0%～2.5% separately in advance in pre-exposure pond and anaerobism or oxygen compatibility pool.Pre-exposure pond controlled temperature is at 15～40 ℃, pH value 6.5～9.0, dissolved oxygen 0.6～3.2mg/L, sludge settling ratio SV
30Be 10%～25%, 12～28 hours residence time, water inlet COD index 1500～3500mg/L, water outlet COD index 400～650mg/L, COD degradation efficiency 40%～75% variously during water outlet is no more than 50mg/L to the influential various indexs of nitrification and denitrification; Through a heavy pond precipitation, mud is back to the pre-exposure pond to the waste water that comes out from the pre-exposure pond, return sludge ratio example 1: 1～1: 2 again.Water collecting basin is collected the water outlet after pre-exposure is handled, and gets to two sections oxygen compatibility pools in the biochemical system with pump again and carries out denitrification denitrogenation, and controlled temperature is at 20～35 ℃, pH value 6.5～7.5, and below the dissolved oxygen 0.5mg/L, sludge settling ratio SV
30Be 15%～30%, 16～24 hours residence time, water inlet COD index 400～650mg/L, water outlet COD index 150～300mg/L, water inlet nitrite and nitrate content are 150～400mg/L.Waste water after oxygen compatibility pool is handled enters Aerobic Pond again and carries out nitrification and further degraded COD, and controlled temperature adds alkali control pH value 6.8～9.0, dissolved oxygen 1.5～4.2mg/L, sludge settling ratio SV at 20～38 ℃
30Be 15%～30%, 24～48 hours residence time, water outlet COD index is 60～150mg/L; Mud in the Aerobic Pond and nitrification liquid are back to oxygen compatibility pool or anaerobic pond, and the overall return sludge ratio example of two sections biochemical systems is 1: 1～1: 5.The waste water that goes out Aerobic Pond is through releasing after second pond is collected mud and refluxed again, to keep the stable of mud in the system.Adopt this treatment process, water outlet can reach national grade one discharge standard under the normal circumstances.The correlationship of each processing parameter and microorganism species addition and COD and ammonia-N removal rate is as shown in the table.
| Group | The microorganism species addition | The gac addition | Temperature ℃ | ?pH | ?SV30 ?％ | Dissolved oxygen mg/l | Pre-exposure residence time h | Two sections residence time h | Total residence time | COD clearance % | Ammonia nitrogen removal frank % | Return sludge ratio |
| The 1st group | ?1.5％ | ?2.5％ | 27 | ?7.5 | ?24 | ?4.2 | ?16 | ?40 | ?60 | ?96 | ?95.7 | ?1∶1 |
| The 2nd group | ?1.8％ | ?2.4％ | 25 | ?7.8 | ?21 | ?3.3 | ?9 | ?48 | ?61 | ?92 | ?96.5 | ?1∶1.2 |
| The 3rd group | ?2.1％ | ?2.2％ | 20 | ?8.2 | ?30 | ?＜0.5 | ?12 | ?50 | ?66 | ?95 | ?94. | ?1∶2.1 |
| The 4th group | ?2.4％ | ?2.0％ | 15 | ?8.5 | ?27 | ?0.8 | ?10 | ?42 | ?56 | ?93 | ?94.4 | ?1∶2.5 |
| The 5th group | ?2.7％ | ?1.8％ | 10 | ?9?0 | ?18 | ?1.5 | ?8 | ?30 | ?42 | ?95.5 | ?98.2 | ?1∶3 |
| The 6th group | ?3.0％ | ?1.6％ | 42 | ?5.0 | ?22 | ?2.2 | ?14 | ?36 | ?54 | ?94 | ?98 | ?1∶3.5 |
| The 7th group | ?3.3％ | ?1.4％ | 35 | ?5.5 | ?20 | ?2.5 | ?18 | ?24 | ?46 | ?94.5 | ?97.6 | ?1∶3.8 |
| The 8th group | ?3.6％ | ?1.2％ | 33 | ?7.0 | ?12 | ?3.0 | ?11 | ?27 | ?42 | ?93.8 | ?96 | ?1∶4 |
| The 9th group | ?4.0％ | ?1.0％ | 30 | ?6.5 | ?15 | ?3.5 | ?17 | ?45 | ?66 | ?92.7 | ?95 | ?1∶5.5 |
Embodiment 4: be used to handle gourmet powder waste water
Gourmet powder waste water acidity is very big, Organic pollutants degree height, and contain the very SO of high density
4 2-And ammonia nitrogen.SO wherein
4 2-With ammonia nitrogen microorganism there is the obvious suppression effect.The most of waste water of at present domestic monosodium glutamate industry can't qualified discharge, handle gourmet powder waste water with HSBEMBM microbial preparation disclosed in this invention, can adopt SBR method or microbial film facture, adopt SBR method treatment effect stable, but continuity is relatively poor, is difficult for adopting control automatically.Adopt biomembrance process, can earlier the HSBEMBM microbial preparation be carried out biofilm and handle, and then waste water is handled, stable through the wastewater effluent that HSBEMBM microbial preparation biofilm is handled, the film difficult drop-off, treatment effect is good.
Embodiment 5: be used to handle antibiotic waste water
Operations such as antibiotic fermentation, extraction, synthetic, exquisiteness all produce a large amount of waste water, metabolism and time meta-bolites that fermenting process is wherein arranged, the chemical that extracts employed special use in the building-up process is arranged, and contain a large amount of acid ions, wherein the product of fermenting process major part is the ring-type macromole, the biochemical treatment difficulty is big, and the variation of osmotic pressure almost can't be survived conventional microorganism.Adopt the HSBEMBM microbial preparation, can be in conjunction with following art breading.
Antibiotic waste water → adjusting settling tank → oxygen treating pond → microbial film aerobic treatment pond → settling tank → the draining of holding concurrently
The HSBEMBM microbial preparation disclosed in this invention of each treating pond volume 1.5%～4.0% of prior interpolation and the gac of each treating pond volume 1.0%～2.5% are wherein all arranged in hold concurrently oxygen treating pond and microbial film aerobic treatment pond.
Hold concurrently oxygen treating pond controlled temperature at 20～35 ℃, pH value 6.5～7.5, below the dissolved oxygen 0.5mg/L, sludge settling ratio SV
30Be 15%～30%, 16～24 hours residence time, microbial film aerobic treatment pond controlled temperature adds alkali control pH value 6.8～9.0, dissolved oxygen 1.5～4.2mg/L, sludge settling ratio SV at 20～38 ℃
30Be 15%～30%, 24～48 hours residence time.
Antibiotic waste water is removed solid matters such as thalline through regulating precipitation process, enter system for anaerobic treatment then, carry out anaerobic treatment, degraded part microbiotic and other organism, carry out aerobic treatment then, can reach discharging standards fully under the waste water normal circumstances after handling through the anaerobic and aerobic microbial film.
Embodiment 6: be used for dye wastewater treatment using
Contain unreacted reactant, by product, dyestuff intermediate, dyestuff in the dyestuff manufacturing processed in the waste water from dyestuff, the complicated component coefficient of dispersion is big, and wherein aromatic hydrocarbons, condensed ring, nitro, aminocompound are typical difficult degradation compositions.Adopt HSBEMBM microbial preparation disclosed in this invention, handle in conjunction with flocculence, can obtain the good treatment effect, technical process is as follows:
Dyestuff (printing and dyeing) waste water → grid processing → flocculation treatment → first Aerobic Pond → oxygen compatibility pool → second Aerobic Pond → water outlet
The HSBEMBM microbial preparation disclosed in this invention of the volume separately 1.5%～4.0% that adds in advance and the gac of each treating pond volume 1.0%～2.5% are wherein respectively arranged in oxygen compatibility pool and first, second Aerobic Pond.The process control condition of oxygen compatibility pool and first, second Aerobic Pond is with embodiment 3.
The clearance of principal pollutant is all higher, average removal rate COD
CrCan reach 95%, BOD
5Be 98%, SS is 95%, and colourity is 94%, and effect is very remarkable.
Embodiment 7: be used to handle black liquid
Adopt the HSBEMBM microbial preparation, in conjunction with following technology:
Black liquor → mistake filtering fiber → acid neutralization → clarification → first Aerobic Pond → anaerobic pond → second Aerobic Pond → draining
The HSBEMBM microbial preparation disclosed in this invention of each the treating pond volume 1.5%～4.0% that adds in advance and the gac of each treating pond volume 1.0%～2.5% are wherein respectively arranged in oxygen compatibility pool and first, second Aerobic Pond.The process control condition of oxygen compatibility pool and first, second Aerobic Pond is with embodiment 3.
Treated black liquor COD clearance can reach more than 95%, and other indexs such as colourity can reach discharging standards, and treatment effect is stable, and expense is low than other treatment processs.
Embodiment 8: be used to handle comprehensive wastewater from chemical industry
Comprehensive wastewater from chemical industry contains category-A, category-B waste water, complicated component, contain organic pollutants such as formaldehyde, methyl alcohol, Caprolactam, methane chloride, pimelinketone, tetramethylolmethane, urotropine, chlorobenzene more, adopt the oxygen of holding concurrently to add the treatment process of aerobic (A-O-A-O), and the HSBEMBM microbial preparation disclosed in this invention of each the treating pond volume 1.5%～4.0% that adds in advance and the gac of each treating pond volume 1.0%～2.5% are arranged in oxygen compatibility pool and Aerobic Pond.The process control condition of oxygen compatibility pool and Aerobic Pond can be obtained the good treatment effect with embodiment 3, and less investment.
Claims (4)
1. be used to handle the microbial preparation of highly difficult waste water, it is characterized in that containing microbial strains below 105 kinds from 47 genus.
Acetobacter aceti acetic acid acetobacter
The Acetobacter liquefaciens acetobacter that liquefies
Acetobacter xylinum acetobacter xylinum
The dry achromobacter of Achromobacter xerosis
Aeromonans hydrophila Aeromonas hydrophila
Aeromonas media Aeromonas media
Aeromonans sobria Aeromonas sobria
Bacillus alvei branch genus bacillus
Bacillus coagulans Bacillus coagulans
Bacillus subtilis subtilis
Bacillus leutis bacillus lentus
Bacillus firmus bacillus firmus
Bacillus mycoides shape genus bacillus
Bacillus megaterium bacillus megaterium
Bacillus alcalophilus Alkaliphilic bacillus
Bacillus cereus bacillus cereus
Bacillus licheniformis Bacillus licheniformis
Bacillus pumilus bacillus pumilus
Bacillus spaericus Bacillus sphaericus
Bacillus marinus bacillus marinus
Alcaligenes denitrificans denitrification subspecies
Alcaligenes faecalis Alcaligenes faecalis
Alcaligenes xylosoxydans Alcaligenes xylosoxidans
Brevibacterium acetylicum acetylene tyrothricin
Brevibacterium ammoniagenes separates the ammonia tyrothricin
Brevibacterium casei brevibacterium casei
The short genus bacillus of Brevibacillus brevis
Enterobacter cloacae enterobacter cloacae
Enterobacter aerogenes enteroaerogen
Enterobacter agglomerans enterobacter agglomerans
Thiobacillus?novellas
Thiobacillus?thioparus
Thiobacillus denitrificans denitrification thiobacillus
Thiobacillus thiooxidans thiobacillus thiooxidant
Thiorhodococcus minus sulphur rhodococcus
Rhodopseudomonas palustris Rhodopseudomonas palustris
Rhodopseudomonas acidphia rhodopseudomonas acidophilus
Gluconobacter albidus Qian Jingshi gluconobacter sp
Gluconobacte oxydans glucose oxidation and bacillus
Lactobacillus fermentum lactobacillus fermentum
Lactobacillus plantarum plant lactobacillus
Lactobacillus alimentarius digests Bacterium lacticum
Lactobacillus amylophillus food starch milk bacillus
Lactobacillus ruminis lactobacillus ruminis
Lactobacillus bervis short lactobacillus
Micrococcus leutus micrococcus luteus
Micrococcus halobius Micrococcus halobius
Pseudomonas alcaligenes Pseudomonas alcaligenes
Pseudomonas aureofaciens causes yellow pseudomonas
Pseudomonas chlororaphis Pseudomonas chlororaphis
The nitrated pseudomonas of Pseudomonas nitroreducens
Pseudomonas riboflavina riboflavin pseudomonas
Pseudomonas putina pseudomonas putida
Pseudomonas facilis Pseudomonas facilis
Paenibacillus gluconolyticus separates the glucan genus bacillus
Paenibacillus thiaminlyticus separates the VitB1 series bacillus
Saccharomyces?telluris
Beggiatoa?alba
Nitrobacter winogradskyi bacterium nitrobacter
Nitrosomonas europaea Nitrosomonas
Nitrosococcus?nitrosus
The narrow and small luminous bacillus of Photobacterium angustum
Photobacterium phosphoreum photobacterium phosphoreum
Photobacterium leiognathi abalone luminous bacillus
The richly endowed bacterium of Haloferax denitrificans denitrification salt
The richly endowed bacterium of Haloferax mediterranei Mediterranean Sea salt
Methanobacterium bryantii Bu Shi methagen
Methanobacterium paluster marsh methagen
Methanobacterium uliginosum mire methagen
Cellulomonas biazotes dinitrogen cellulomonas cartae
Cellulomonas fimi muck cellulomonas cartae
Kurthia zopfii Zuo Shi Al Kut Salmonella
Thiosphaera?pantotropha
Alcaligenes?sp
Chlorobium limicola mud is given birth to green bacterium
The long red bacterium of Erythrobacter longus
The red Zymomonas mobilis of Erothromonas ursincola
Azomonas macrocytogenes giant cell nitrogen Zymomonas mobilis
The yellow bacillus flavus of Xanthobacter flavus
Methylcoccus capsulatus pod membrane methyl coccus
Alteromonas denitrificans denitrification replaces Zymomonas mobilis
Alteromonas nigrifaciens produces black alternately Zymomonas mobilis
Telluria mxita mixes the refreshing bacterium in ground
Bacteroides cellulosovens Bacteroides cellulosolvens
Bacteroides stercoris Bacteroides stercoris
Many nutritive muds of Ilyobacter ploytropus bacillus
The false butyric acid vibrios of Pseudobutyrivibrio ruminis cud
Zymomonas mobilis is supported in Syntrophomonas wolfei Ao Shi syntrophism
The simple pimelobacter sp of Pimelobacter simplex
Pimelobacter tumescens swelling pimelobacter sp
The short shape bacillus of Brachybacterium faecium excrement
Jonesia denitrificans denitrification Jones Salmonella
The rotten rare bacillus of Rarobacter faecitabidus slag
Eubacterium formicigenerans Eubacterium formicigenerans
Eubacterium nitritogenes Eubacterium nitrotogenes
Eubacterium xylanophilum has a liking for the polyxylose Eubacterium
The aurantia small bacillus of Exiguobacterium aurantiacum
Bacillus is supported in Synteophobacter wolinii Wo Shi syntrophism
Bacillus is supported in Synteophobacter pnnigii Fen Shi syntrophism
Pelobacter acetylenicus acetylene occupies the mud bacillus
Pelobacter propionicus produces propionic acid and occupies the mud bacillus
Thiodictyon elegans U.S. Open Tennis sulphur bacterium
The purple capsule sulphur of Thiocystis violacea bacterium
Anaerovibrio glycerini glycerine anaerobism vibrios
Anaerovibrio lipolytica Anaerovibrio lipolytica
2. be used to handle the preparation method of the microbial preparation of highly difficult waste water; it is characterized in that it being after the process microbe to screen is handled with domestication in the laboratory with active sludge; bacterial classification after the screening is mixed; and contain 105 kinds of bacterial strains as claimed in claim 1 in the bacterial classification mixture after guaranteeing to be mixed; bacterial classification after being mixed adopts aerobic respectively and anaerobism is cultivated; bacterial classification after will cultivating is again cultivated bacterial classification by anaerobism: aerobic cultivation bacterial classification is 1: 3 a mixed, adds stablizer and bacterial classification protective material.
3. the preparation method who is used to handle the microbial preparation of highly difficult waste water as claimed in claim 2, it is characterized in that three kinds of substratum of aerobic cultivation employing, be respectively the bacterium perfect medium, the glucose perfect medium, the cane sugar inorganic salt substratum, three kinds of substratum carry out inoculation culture respectively, culture condition is 30～32 ℃, 120 rev/mins, time is 24～36 hours, cultivate and finish after 1: 1: 1 mixed is pressed in check, anaerobic culture medium adopts the glucose perfect medium, culture temperature is 32～35 ℃, every two hours stirs 5 minutes, and incubation time is 48 hours.
4. the preparation method who is used to handle the microbial preparation of highly difficult waste water as claimed in claim 2 is characterized in that the bacterium perfect medium is a beef-protein medium.
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| CN 200410005196 CN1563342A (en) | 2004-02-10 | 2004-02-10 | Microbe preparation in use for treating high difficult wastewater and preparation method |
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| CN 200410005196 CN1563342A (en) | 2004-02-10 | 2004-02-10 | Microbe preparation in use for treating high difficult wastewater and preparation method |
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