CN109486718A - Complex microbial inoculum and preparation and application for wash water processing of weaving - Google Patents
Complex microbial inoculum and preparation and application for wash water processing of weaving Download PDFInfo
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- CN109486718A CN109486718A CN201811533087.3A CN201811533087A CN109486718A CN 109486718 A CN109486718 A CN 109486718A CN 201811533087 A CN201811533087 A CN 201811533087A CN 109486718 A CN109486718 A CN 109486718A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
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Abstract
The invention discloses a kind of complex microbial inoculums and preparation and application for wastewater treatment after wash water materialization of weaving, the microbial inoculum includes: bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, micrococcus luteus, Lactococcus lactis, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus, Sphingobacterium.CODcr, ammonia nitrogen in water can be effectively reduced by biological reinforcing technology in the complex micro organism fungicide, reduce the processing load of backend process, improve treatment effeciency, reduce cost.
Description
Technical field
The invention belongs to textile waste processing technology fields, at waste water after a kind of wash water materialization that is used to weave
The complex microbial inoculum and preparation and application of reason.
Background technique
Clothes laundering and dyeing trade is novel industry increasingly burning hoter in recent years.Laundry service is as the society's clothes to match with dress ornament
Business industry, it is closely bound up with daily life.With economic development and improvement of living standard, socialization is whole wash it is more next
More, increasingly scale is bound to cause a large amount of laundrywastes, contain in laundrywastes there are many rich nutritive material, without
Direct emission is handled, water eutrophication certainly will be will cause, destroys aquatic ecological balance.So in the shape of current shortage of water resources
Under gesture, processing is carried out to the waste water of laundry factory and is very necessary.
As the improvement of people's living standards, more and more laundry factories appear in city.Laundry factory is mostly with laundry
Based on powder and liquid detergent, oils, the fatty acid, phosphorus of more surfactant and high concentration are often contained in waste water
Hydrochlorate and other synthetic detergents add object ingredient.Laundrywastes mainly includes washes, cleaning waste water and drying waste water.It is each useless
Water has the characteristics that different: containing a large amount of staple fibers and foams of washing powder in laundrywastes, CODcr value is higher, more muddy, normal band
There is very deep color；It is big to clean wastewater flow rate, has a small amount of foam, contained suspended matter is less, and CODcr value is also smaller, relatively transparent；It gets rid of
Dry wastewater flow rate is small, and it is often mixed laundrywastes in real life that water quality, which is slightly better than, which cleans waste water,.
By screening, tame, be building up to the complex microbial inoculum of practical application, by laboratory shake flask experiment, lab scale,
Process of the pilot scale to industrial experiment, it is ensured that the feasibility for the treatment of process and the high efficiency of bacteria agent.Therefore, microorganism is compound
Microbial inoculum applies to wastewater treatment mesophytization process section, is the main trend of laundrywastes development.
Summary of the invention
It efficient, nontoxic, biodegradable is used for it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
The complex microbial inoculum and preparation and application of wastewater treatment after weaving wash water materialization.
In order to achieve the above objectives, the technical scheme is that
A kind of complex microbial inoculum for wastewater treatment after wash water materialization of weaving, which is characterized in that by following microbial inoculum
It is formulated by bacterium solution volume ratio:
Bacillus subtilis 8%-16%；
Bacillus licheniformis 8%-16%；
Bacillus amyloliquefaciens subspecies 8%-16%；
Micrococcus luteus 8%-16%；
Lactococcus lactis 4%-10%；
Acetobacter 4%-10%；
Klebsiella 4%-10%；
Bacillus 4%-10%；
Saccharomycete 4%-10%；
Bacillus cereus 2%-8%；
Bacillus flexus 10%-20%；
Sphingobacterium 10%-20%.
The present invention also provides a kind of preparation sides for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving
Method, comprising the following steps:
S1- strain prepare: bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, micrococcus luteus,
Lactococcus lactis, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus, sheath ammonia
Each one plant of the strain of alcohol bacillus；
S2- actication of culture: by bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, M. luteus
Bacterium, Lactococcus lactis, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus,
Sphingobacterium is activated with fluid nutrient medium into original seed respectively；
S3- expands culture: the original seed bacterium solution of activation is inoculated into respectively in corresponding culture medium and carries out shaken cultivation, then
By fermentor batch fermentation, when microbial total bacterium number is greater than 109When CFU/ml, the bacterium solution of each strain is collected simultaneously using storage tank
Storage tank is closed, it is saved under conditions of being placed in environment temperature lower than 20 DEG C；
S4- matches bacteriostat: the bacterium solution of collection is configured to complex micro organism fungicide by volume.
The present invention also provides the application methods of mentioned microorganism composite bacteria agent: wastewater treatment after to weaving wash water materialization
Biochemical reaction tank inlet adds complex microbial inoculum, and dosage is determined according to waste water water draw rate after weaving wash water materialization,
It is generally continuously added in 0.1~0.5% ratio of water draw rate, more active in summer microorganism growth metabolism, microorganism is multiple
The dosage of combined bacteria agent can be reduced, and generally continuously be added in 0.05~0.1% ratio of water draw rate.
This programme the utility model has the advantages that (1) complex microbial inoculum is made of a variety of environmentally friendly microorganisms.It is making
In the process, the metabolite of microorganism is not extracted, this mode has not only saved cost, but also keeps depositing for viable bacteria
Be conducive to the utilization rate of viable bacteria and merged with activated sludge in reaction tank is supported well.(2) after biochemical reaction tank adds microbial inoculum, energy
The stability of raising system, impact resistance enhancing, good denitrification dephosphorization effect are less prone to sludge bulking, it can be achieved that sewage
High treating effect, Treatment of Sludge are easy.(3) the effective viable bacteria composition contained in complex microbial inoculum can use weaving wash water
Assimilable organic carbon does not only reach the effect for removing organic matter as nutrient source in after materialization, and by the life of microorganism
Life activity generates metabolite, can effectively purify water.(4) complex microorganism can generate a variety of degrading enzymes, advantage point
Carbon system, the nitrogen system, phosphorus system, sulphur system pollutant in water body are solved, forms good population structure in activated sludge, improves biochemical system
Processing capacity.
Specific embodiment
Technical solution in the present invention is clearly and completely described, it is clear that described embodiment is only the present invention
Preferred embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of complex microbial inoculum for wastewater treatment after wash water materialization of weaving presses bacterium solution volume ratio by following microbial inoculum
It is formulated:
Bacillus subtilis 16%；
Bacillus licheniformis 16%；
Bacillus amyloliquefaciens subspecies 10%；
Micrococcus luteus 10%；
Lactococcus lactis 4%；
Acetobacter 4%；
Klebsiella 4%；
Bacillus 4%；
Saccharomycete 4%；
Bacillus cereus 4%；
Bacillus flexus 12%；
Sphingobacterium 12%.
Embodiment 2
A kind of complex microbial inoculum for wastewater treatment after wash water materialization of weaving presses bacterium solution volume ratio by following microbial inoculum
It is formulated:
Bacillus subtilis 16%；
Bacillus licheniformis 16%；
Bacillus amyloliquefaciens subspecies 10%；
Micrococcus luteus 10%；
Lactococcus lactis 4%；
Acetobacter 4%；
Klebsiella 4%；
Bacillus 4%；
Saccharomycete 4%；
Bacillus cereus 4%；
Bacillus flexus 12%；
Sphingobacterium 12%.
Embodiment 3
A kind of complex microbial inoculum for wastewater treatment after wash water materialization of weaving presses bacterium solution volume ratio by following microbial inoculum
It is formulated:
Bacillus subtilis 10%；
Bacillus licheniformis 10%；
Bacillus amyloliquefaciens subspecies 14%；
Micrococcus luteus 14%；
Lactococcus lactis 5%；
Acetobacter 5%；
Klebsiella 6%；
Bacillus 6%；
Saccharomycete 3%；
Bacillus cereus 6%；
Bacillus flexus 8%；
Sphingobacterium 13%.
A kind of preparation method for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving, including following step
It is rapid:
S1- strain prepare: bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, micrococcus luteus,
Lactococcus lactis, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus, sheath ammonia
Each one plant of the strain of alcohol bacillus；
S2- actication of culture: by bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, M. luteus
Bacterium, Lactococcus lactis, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus,
Sphingobacterium is activated with fluid nutrient medium into original seed respectively；
Wherein, bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, micrococcus luteus, saccharomycete,
Bacillus cereus, Bacillus flexus, Sphingobacterium can be activated with LB liquid medium into original seed；Lactococcus lactis,
Acetobacter can be activated with acid-producing bacteria fluid nutrient medium into original seed；It is Klebsiella, bacillus, bacillus cereus, curved
Bent bacillus, Sphingobacterium can be activated with aerobic denitrification fluid nutrient medium into original seed, and saccharomycete can use brewer's wort
Fluid nutrient medium is activated into original seed；
S3- expands culture: the original seed bacterium solution of activation is inoculated into respectively in above-mentioned corresponding culture medium and carries out shaken cultivation,
Again by fermentor batch fermentation, when microbial total bacterium number is greater than 109When CFU/ml, the bacterium solution of each strain is collected using storage tank
And storage tank is closed, it is saved under conditions of being placed in environment temperature lower than 20 DEG C；
S4- matches bacteriostat: the bacterium solution of collection is configured to complex micro organism fungicide by volume.
A kind of application method for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving, to weaving wash water object
The biochemical reaction tank inlet of wastewater treatment adds complex microbial inoculum after change, and dosage is according to waste water after weaving wash water materialization
Water draw rate determine, generally continuously added in 0.1~0.5% ratio of water draw rate, summer microorganism growth metabolism more
Active, the dosage of complex microbial inoculum can be reduced, and generally continuously be added in 0.05~0.1% ratio of water draw rate.
The application experiment case of this complex microbial inoculum: it after to weaving wash water materialization in wastewater treatment simulator, presses
It is continuously added according to 0.05% ratio of water draw rate, experiment wastewater is certain weaving laundry factory waste water.It is compound to microorganism is added
Before microbial inoculum, system stablize after water quality CODcr, NH3- N is monitored and detects, referring to table 1:
Table 1 adds the front and back index (average value) of complex microbial inoculum
As can be seen from Table 1: after adding complex microbial inoculum, the removal rate of CODcr has reached 83.02%, simultaneously
The removal rate of NH3-N has also reached 97.69%.The CODcr and NH3-N in textile waste can be effectively removed, rear end is by sterilization
After processing, can qualified discharge standard meet I grade of " textile dyeing and finishing industrial water pollution object discharge standard " (GB 4287-1992)
Discharge standard.
After wash water materialization of weaving in wastewater treatment process, waste water can be effectively reduced by adding complex microbial inoculum
CODcr, and reach 83.02% removal rate, fully meet wastewater treatment requirement after weaving wash water materialization.Simultaneously to weaving wash water
The NH of waste water after materialization3- N has good removal rate.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of complex microbial inoculum for wastewater treatment after wash water materialization of weaving, which is characterized in that pressed by following microbial inoculum
Bacterium solution volume ratio is formulated:
Bacillus subtilis 8%-16%；
Bacillus licheniformis 8%-16%；
Bacillus amyloliquefaciens subspecies 8%-16%；
Micrococcus luteus 8%-16%；
Lactococcus lactis 4%-10%；
Acetobacter 4%-10%；
Klebsiella 4%-10%；
Bacillus 4%-10%；
Saccharomycete 4%-10%；
Bacillus cereus 2%-8%；
Bacillus flexus 10%-20%；
Sphingobacterium 10%-20%.
2. the complex microbial inoculum according to claim 1 for wastewater treatment after wash water materialization of weaving, feature exist
In: it is formulated by following microbial inoculum by bacterium solution volume ratio:
Bacillus subtilis 16%；
Bacillus licheniformis 16%；
Bacillus amyloliquefaciens subspecies 10%；
Micrococcus luteus 10%；
Lactococcus lactis 4%；
Acetobacter 4%；
Klebsiella 4%；
Bacillus 4%；
Saccharomycete 4%；
Bacillus cereus 4%；
Bacillus flexus 12%；
Sphingobacterium 12%.
3. the complex microbial inoculum according to claim 1 for wastewater treatment after wash water materialization of weaving, feature exist
In: it is formulated by following microbial inoculum by bacterium solution volume ratio:
Bacillus subtilis 12%；
Bacillus licheniformis 12%；
Bacillus amyloliquefaciens subspecies 12%；
Micrococcus luteus 12%；
Lactococcus lactis 6%；
Acetobacter 6%；
Klebsiella 5%；
Bacillus 5%；
Saccharomycete 5%；
Bacillus cereus 5%；
Bacillus flexus 10%；
Sphingobacterium 10%.
4. the complex microbial inoculum according to claim 1 for wastewater treatment after wash water materialization of weaving, feature exist
In: it is formulated by following microbial inoculum by bacterium solution volume ratio:
Bacillus subtilis 10%；
Bacillus licheniformis 10%；
Bacillus amyloliquefaciens subspecies 14%；
Micrococcus luteus 14%；
Lactococcus lactis 5%；
Acetobacter 5%；
Klebsiella 6%；
Bacillus 6%；
Saccharomycete 3%；
Bacillus cereus 6%；
Bacillus flexus 8%；
Sphingobacterium 13%.
5. a kind of preparation method for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving, which is characterized in that packet
Include following steps:
S1- strain prepares: bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, micrococcus luteus, lactic acid
Galactococcus, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus, sphingol bar
Each one plant of the strain of bacterium；
S2- actication of culture: by bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens subspecies, micrococcus luteus, cream
Yogurt coccus, acetobacter, Klebsiella, bacillus, saccharomycete, bacillus cereus, Bacillus flexus, sphingol
Bacillus is activated with fluid nutrient medium into original seed respectively；
S3- expands culture: the original seed bacterium solution of activation is inoculated into respectively in corresponding culture medium and carries out shaken cultivation, then is passed through
Fermentor batch fermentation, when microbial total bacterium number is greater than 109When CFU/ml, the bacterium solution of each strain is collected using storage tank；
S4- matches bacteriostat: the bacterium solution of collection is configured to complex micro organism fungicide by volume.
6. the preparation side according to claim 5 for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving
Method, which is characterized in that in step s3, the storage tank for collecting bacterium solution is closed, saves under conditions of being placed in environment temperature lower than 20 DEG C.
7. a kind of application method for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving, which is characterized in that
The biochemical reaction tank inlet of wastewater treatment adds complex microbial inoculum after weaving wash water materialization.
8. the user according to claim 7 for the complex microbial inoculum of wastewater treatment after wash water materialization of weaving
Method, which is characterized in that the dosage of complex microbial inoculum is continuously added in the ratio of the 0.05-0.5% of water draw rate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113136351A (en) * | 2021-04-25 | 2021-07-20 | 重庆融极浩瀚生物技术有限公司 | Composite microbial flocculant and preparation method and application thereof |
| CN114437954A (en) * | 2020-11-03 | 2022-05-06 | 中国石油天然气集团有限公司 | Bacillus flexus for degrading sulfonated drilling fluid, mixed bacteria and application of bacillus flexus |
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| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
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2018
- 2018-12-14 CN CN201811533087.3A patent/CN109486718B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563342A (en) * | 2004-02-10 | 2005-01-12 | 凌亮 | Microbe preparation in use for treating high difficult wastewater and preparation method |
| CN108101228A (en) * | 2017-12-13 | 2018-06-01 | 浙江海洋大学 | A kind of wastewater treatment microbial microbial inoculum |
Non-Patent Citations (4)
| Title |
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| CHAO-QUN DING ET AL: "Study on community structure of microbial consortium for the degradation of viscose fiber wastewater", 《BIORESOUR BIOPROCESS》 * |
| 王慧荣等: "微生物菌剂对印染废水强化处理的效果及活性污泥菌群的变化", 《工业用水与废水》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114437954A (en) * | 2020-11-03 | 2022-05-06 | 中国石油天然气集团有限公司 | Bacillus flexus for degrading sulfonated drilling fluid, mixed bacteria and application of bacillus flexus |
| CN113136351A (en) * | 2021-04-25 | 2021-07-20 | 重庆融极浩瀚生物技术有限公司 | Composite microbial flocculant and preparation method and application thereof |
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