CN103409404B - Biological preparation for treating domestic sewage - Google Patents

Biological preparation for treating domestic sewage Download PDF

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Publication number
CN103409404B
CN103409404B CN201310374623.0A CN201310374623A CN103409404B CN 103409404 B CN103409404 B CN 103409404B CN 201310374623 A CN201310374623 A CN 201310374623A CN 103409404 B CN103409404 B CN 103409404B
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China
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described
bacillus
mixed
phanerochaete chrysosporium
pseudomonas stutzeri
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CN201310374623.0A
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Chinese (zh)
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CN103409404A (en
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闫鹏
高建兰
赵月元
应通
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山东元通监测有限公司
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Abstract

The invention belongs to the technical field of environment friendliness, and discloses a biological preparation for treating domestic sewage. The biological preparation is formed by mixing a mixed bacterium liquid and an auxiliary preparation material at the weight ratio of 1:1. The invention further discloses a method for treating the domestic sewage via the biological preparation. In the biological preparation, various microorganisms are matched to perform actions of symbiotic coordination and cannot generate mutual antagonism; various pollutants in the sewage can be effectively removed.

Description

A kind of biotechnological formulation for the treatment of sanitary sewage

Technical field

The invention belongs to environmental technology field, be specifically related to a kind of biotechnological formulation for the treatment of sanitary sewage.

Background technology

Sanitary sewage is the sewage producing in human lives's process, is one of primary pollution source of water body.Mainly ight soil and wash water.The sanitary sewage amount that discharge everyone every day in city is 150-400L, and its amount has substantial connection with standard of living.In sanitary sewage, contain large amount of organic, as Mierocrystalline cellulose, starch, carbohydrate and fat protein etc.; Also often contain pathogenic bacteria, virus and parasitic ovum; The muriate of inorganic salts, vitriol, phosphoric acid salt, supercarbonate and sodium, potassium, calcium, magnesium etc.Total feature is nitrogenous, sulfur-bearing and phosphorous height, and post-processed is more difficult.People should water conservation. and China's actual conditions are more special, and water resources occupancy volume per person is few, and spatial distribution is uneven.Along with Chinese Urbanization's, industrialized acceleration, the demand gap of water resources also increases day by day.Under such background, sewage treatment industry becomes new industry, at present and tap water production, water supply, draining, middle water reuse industry in status of equal importance.

The main method of the pollutents such as sulphur phosphorus ammonia nitrogen in prior art in conventional removal sewage has: Physical, chemical method, biological process.Physical is containing treatment technologies such as reverse osmosis, distillation, soil irrigations; Chemical method is containing treatment technologies such as ion-exchange, ammonia stripping, break point chlorination, burning, chemical precipitation, catalytic pyrolysis, electrodialysis, electrochemistry; Biological process is containing treatment technologies such as algal culture, biological nitration, immobilized biotechnologies.Practical method has at present: break point chlorination method, selective ion exchange method, ammonia blow-off method, biological process and chemical precipitation method.Wherein, break point chlorination method, selective ion exchange method, ammonia blow-off method are not suitable for large-scale industrialization application, and chemical process easily causes that other pollute, and cost is higher, and the method for the most economic environmental protection is at present biological process.Prior art is in the urgent need to a kind of simple biotechnological formulation efficient for processing sanitary sewage.

Summary of the invention

In order to overcome the deficiencies in the prior art, efficiently process simply sanitary sewage, the invention provides a kind of biotechnological formulation for the treatment of sanitary sewage, its technical scheme realizes by the following method:

For the treatment of a biotechnological formulation for sanitary sewage, it is prepared from by the following method: mixed bacteria liquid and pharmaceutical adjunct are mixed according to the weight ratio of 1:1, stir, then put cryodrying at 4-6 ℃, dry rear water content is controlled at 68%, obtains; Described microbial inoculum carrier is mixed to get according to the mass ratio of 4:2:1 by sawdust, diatomite and chitosan; Described composite bacteria liquid is mixed by the raw material bacterium of following weight percent:

Bacillus pumilus 20%, bacillus cereus 18%, Arthrobacter globiformis 16%, Pseudomonas stutzeri 12%, sphingosine sporangium 10%, thiobacillus denitrificans 8%, Phanerochaete chrysosporium 6%, Bacillus licheniformis 6%, Chryseobacterium sp 4%; The concentration of above-mentioned raw materials bacterium is all controlled at 1 * 10 8individual/ml.

Preferably,

Described bacillus pumilus be bacillus pumilus (Bacillus pumilus) ATCC27142 (can be referring to International Journal of Pharmaceutics, Volume160, Issue1,1998, Pages75-81);

Described bacillus cereus be bacillus cereus (Bacillus cereus) ATCC10876 (NewJ.Chem. for example, 2007,31,748-755).

Described Arthrobacter globiformis is Arthrobacter globiformis (Arthrobacter globiformi s) CCTCC NO:M209015 (CN101492649A);

Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107 (referring to CN101705202A);

Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589 (visible CN102168054A);

Described thiobacillus denitrificans be thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259 (reference APPLIED AND ENVIRONMENTAL MICROBIOLOGY for example, May2007, p.3265-3271);

Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725 (for example APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714);

Described Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) CCTCC NO.M206082 (visible CN101037659A);

The preferred Chryseobacterium sp of described Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282 (visible CN102433277A).

Bacterium of the present invention all can be bought and obtain from commercial sources such as CGMCC, CCTCC and US mode culture collection warehousings (ATCC).

The enlarged culturing of each bacterial classification of the present invention is the cellar culture mode of this area, and the training method of recording in also can reference literature obtains.

The invention also discloses a kind of Technology of utilizing above-mentioned biotechnological formulation to process sanitary sewage.

The main beneficial effect that the present invention obtains is as follows:

The present invention has selected suitable bacterial classification by test of many times, carries out compatibility, environment friendly and pollution-free, with low cost, reasonable compatibility between each bacterial classification in microbiobacterial agent, symbiosis is coordinated, antagonism not mutually, its preparation method is easy, easy to implement the method, it is easy and simple to handle, is beneficial to suitability for industrialized production; The present invention utilizes biotechnological formulation to process sanitary sewage, has reduced the input of fixed capital, has reduced cost, has avoided the wasting of resources; Treatment process of the present invention has purified sanitary sewage effectively, and phosphorus and ammonia nitrogen remove simultaneously, has solved the contradiction that in traditional technology, dephosphorization and denitrification effect are difficult to take into account simultaneously.

Embodiment

By the mode that adopts specific embodiment, the present invention is further elaborated below, but it should not be construed the restriction to core initiative spirit of the present invention.

Embodiment 1

For the treatment of a biotechnological formulation for sanitary sewage, it is prepared from by the following method: mixed bacteria liquid and pharmaceutical adjunct are mixed according to the weight ratio of 1:1, stir, then put cryodrying at 6 ℃, dry rear water content is controlled at 8%, obtains; Described microbial inoculum carrier is mixed to get according to the mass ratio of 4:2:1 by sawdust, diatomite and chitosan; Described composite bacteria liquid is mixed by the raw material bacterium of following weight percent: bacillus pumilus 20%, bacillus cereus 18%, Arthrobacter globiformis 16%, Pseudomonas stutzeri 12%, sphingosine sporangium 10%, thiobacillus denitrificans 8%, Phanerochaete chrysosporium 6%, Bacillus licheniformis 6%, Chryseobacterium sp 4%; The concentration of above-mentioned raw materials bacterium is all controlled at 1 * 10 8individual/ml.

Using method: 10 grams of biotechnological formulations that at every turn add above-mentioned preparation according to every cubic metre of liquid, add every day 1 time, add continuously one week, the last standing clearance that detects afterwards pollutent in water sample for 5 days: the clearance of ammonia nitrogen and sulphur is respectively 99%, can't detect phosphor contaminant.

Embodiment 2

For the treatment of a biotechnological formulation for sanitary sewage, it is prepared from by the following method: mixed bacteria liquid and pharmaceutical adjunct are mixed according to the weight ratio of 1:1, stir, then put cryodrying at 4 ℃, dry rear water content is controlled at 6%, obtains; Described microbial inoculum carrier is mixed to get according to the mass ratio of 4:2:1 by sawdust, diatomite and chitosan; Described composite bacteria liquid is mixed by the raw material bacterium of following weight percent: bacillus pumilus 20%, bacillus cereus 18%, Arthrobacter globiformis 16%, Pseudomonas stutzeri 12%, sphingosine sporangium 10%, thiobacillus denitrificans 8%, Phanerochaete chrysosporium 6%, Bacillus licheniformis 6%, Chryseobacterium sp 4%; The concentration of above-mentioned raw materials bacterium is all controlled at 1 * 10 8individual/ml; Described bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142; Described bacillus cereus is bacillus cereus (Bacillus cereus) ATCC10876; Described Arthrobacter globiformis is Arthrobacter globiformis (Arthrobacter globiformis) CCTCC NO:M209015; Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107; Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589; Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259; Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725; Described Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) CCTCC NO.M206082; Described Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282.

Using method: sanitary sewage is selected near sewer line community, Lanshan District, according to every cubic metre of liquid, add 10 grams of the biotechnological formulations of above-mentioned preparation at every turn, add every day 1 time, add continuously one week, the last standing removal effect that detects afterwards pollutent in water sample for 5 days, pollutant load comparison before and after the sewage of processing, in Table 1:

Table 1

Project name Water quality before processing Water quality after processing CODcr 2700mg/L 17mg/L NH3-N 130mg/L 0.9mg/L S 29mg/L 0.2mg/L P 21mg/L 0.02mg/L

Although, above with general explanation and embodiment, this case having been done to detailed explanation, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the modification done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.

Claims (1)

1. for the treatment of a biotechnological formulation for sanitary sewage, it is prepared from by the following method: mixed bacteria liquid and pharmaceutical adjunct are mixed according to the weight ratio of 1:1, stir, then put cryodrying at 4-6 ℃, dry rear water content is controlled at 6-8%, obtains; Described pharmaceutical adjunct is mixed to get according to the mass ratio of 4:2:1 by sawdust, diatomite and chitosan; Described mixed bacteria liquid is mixed by the raw material bacterium of following weight percent: bacillus pumilus 20%, bacillus cereus 18%, Arthrobacter globiformis 16%, Pseudomonas stutzeri 12%, sphingosine sporangium 10%, thiobacillus denitrificans 8%, Phanerochaete chrysosporium 6%, Bacillus licheniformis 6%, Chryseobacterium sp 4%;
Described bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142; Described bacillus cereus is bacillus cereus (Bacillus cereus) ATCC10876; Described Arthrobacter globiformis is Arthrobacter globiformis (Arthrobacter globiformis) CCTCC NO:M209015; Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107; Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589; Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259; Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725; Described Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) CCTCC NO.M206082; Described Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282.
CN201310374623.0A 2013-08-23 2013-08-23 Biological preparation for treating domestic sewage CN103409404B (en)

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CN104528955B (en) * 2015-01-22 2016-08-24 曹俊萍 A kind of microorganism formulation for repairing chemical fertilizer factory's sewage
CN104528956B (en) * 2015-01-22 2016-08-24 马金晔 A kind of composite bacteria agent capable for administering coal chemical industry sewage
CN106310320A (en) * 2015-06-15 2017-01-11 仁维国际股份有限公司 Deodorant and application thereof
CN105779344A (en) * 2016-03-29 2016-07-20 江苏蓝星化工环保股份有限公司 Composite microbial agent
CN106862260A (en) * 2017-03-10 2017-06-20 刘晨 A kind of biochemical process for administering Cadmium Pollution in Soils

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US6325934B1 (en) * 2000-05-09 2001-12-04 James Francis Tobey, Jr. Enzyme and bacterial combination in a slowly dissolvable matrix for septic tanks, grease traps and waste treatment
CN100500831C (en) * 2003-02-14 2009-06-17 中国科学院南京土壤研究所 Denitrifying bacteria composition and its application
GB0407329D0 (en) * 2004-03-31 2004-05-05 Danisco Process
CN101570366B (en) * 2009-06-12 2012-05-30 北京金源化学集团有限公司 Compound liquid for enhancing sewage biological treatment
CN102492642B (en) * 2011-12-20 2013-04-17 江南大学 Denitrifying strain with nitrifying function, strain-containing water body improver of multiple active microorganisms and preparation method of water body improver

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