CN103449681B - Method for treating sewage by using microbial preparation - Google Patents

Method for treating sewage by using microbial preparation Download PDF

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Publication number
CN103449681B
CN103449681B CN201310419226.0A CN201310419226A CN103449681B CN 103449681 B CN103449681 B CN 103449681B CN 201310419226 A CN201310419226 A CN 201310419226A CN 103449681 B CN103449681 B CN 103449681B
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liquid
sewage
parts
microbial
mixed
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CN201310419226.0A
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CN103449681A (en
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何洋
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何洋
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Abstract

The invention belongs to the technical field of environmental protection and discloses a method for treating sewage by using a microbial preparation. The method comprises the steps of preparing the microbial preparation, pre-treating the sewage, carrying out microbial oxidation, and the like. The invention further discloses the microbial preparation for sewage treatment. The method disclosed by the invention has the advantages that the operating methods are simple, the investment is small, and pollutants, such as ammonia, nitrogen, sulfur, phosphorus and the like, in the sewage can be effectively removed.

Description

A kind of method of utilizing microbial preparation to dispose of sewage
Technical field
The invention belongs to environmental technology field, be specifically related to a kind of method of utilizing microbial preparation to dispose of sewage.
Background technology
Sewage, be often referred to be subject to certain that pollute, from life and the waste water produced.Sewage mainly contains sanitary sewage and trade effluent.In the water pollution that human production activity causes, the water pollution that industry causes is the most serious.As trade effluent, it is many containing pollutent, and complicated component is not only difficult for purifying, and processes also more difficult in water.Trade effluent is the most important reason that industrial pollution causes water pollution.It accounts for the major part of the pollutent of industry discharge.The contained pollutent of trade effluent varies because factory's kind is different, even same class factory, production process is different, and the quality and quantity of its contained pollutent is also different.The industrial waste water except discharge directly injects water body to be caused pollution, and solid waste and waste gas also can polluted-waters.Sanitary sewage is the sewage producing in human lives's process, is one of primary pollution source of water body.Mainly ight soil and wash water.The sanitary sewage amount that discharge everyone every day in city is 150-400L, and its amount has substantial connection with standard of living.In sanitary sewage, contain large amount of organic, as Mierocrystalline cellulose, starch, carbohydrate and fat protein etc.; Also often contain pathogenic bacteria, virus and parasitic ovum; The muriate of inorganic salts, vitriol, phosphoric acid salt, supercarbonate and sodium, potassium, calcium, magnesium etc.Total feature is nitrogenous, sulfur-bearing and phosphorous height, and post-processed is more difficult.People should water conservation. and China's actual conditions are more special, and water resources occupancy volume per person is few, and spatial distribution is uneven.Along with Chinese Urbanization's, industrialized acceleration, the demand gap of water resources also increases day by day.Under such background, sewage treatment industry becomes new industry, at present and tap water production, water supply, draining, middle water reuse industry in status of equal importance.
Sulphur phosphorus ammonia nitrogen is the principal pollutant in sewage, is also modal pollutent.The main method of the pollutents such as sulphur phosphorus ammonia nitrogen in prior art in conventional removal sewage has: Physical, chemical method, biological process.Physical is containing treatment technologies such as reverse osmosis, distillation, soil irrigations; Chemical method is containing treatment technologies such as ion-exchange, ammonia stripping, break point chlorination, burning, chemical precipitation, catalytic pyrolysis, electrodialysis, electrochemistry; Biological process is containing treatment technologies such as algal culture, biological nitration, immobilized biotechnologies.Practical method has at present: break point chlorination method, selective ion exchange method, ammonia blow-off method, biological process and chemical precipitation method.Wherein, break point chlorination method, selective ion exchange method, ammonia blow-off method are not suitable for large-scale industrialization application, and chemical process easily causes that other pollute, and cost is higher, and the method for the most economic environmental protection is at present microbial method.Prior art is in the urgent need to a kind of effectively dispose of sewage method of the pollutents such as middle ammonia nitrogen sulphur phosphorus of microbial preparation of utilizing.
Summary of the invention
In order to overcome the deficiencies in the prior art, effectively remove simply the pollutents such as ammonia nitrogen sulphur phosphorus in sewage, the invention provides a kind of method of utilizing microbial preparation to dispose of sewage, its technical scheme realizes by the following method:
A method of utilizing microbial preparation to dispose of sewage, it comprises the steps:
The preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, standing 6-8 hour then, cryodrying at the most rearmounted 4 ℃, dry after water content be controlled at 6%, obtain; By bamboo charcoal and chitosan, the mass ratio according to 1: 1 is mixed to get described carrier, and the particle diameter of bamboo charcoal is 10 orders preferably;
Sewage pre-treatment: first, by sewage process solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitates 12 hours, then liquid is removed to solid throw out by circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm
Microbiological oxidation: the liquid through circular hole filtering net enters microbial reaction pond, regulating pH is 7, by every cubic metre of liquid, adds 10 grams of microbial preparations at every turn, adds every day 1 time, adds continuously one week, last standing 3 days, liquid is discharged.
Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part:
10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps; The concentration of above-mentioned each raw material bacterium is all controlled at (1-2) * 10 8individual/ml.Above-mentioned bacterial classification can be the conventional bacterial strain of prior art,
Preferably,
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259 (reference APPLIED AND ENVIRONMENTAL MICROBIOLOGY for example, May 2007, p.3265-327 1);
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107 (for example CN101705202A);
Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589 (for example CN102168054A);
Described bacillus pumilus be bacillus pumilus (Bacilhs pumilus) ATCC 27142 (International Journal of Pharmaceutics for example, Volume 160, Issue 1,1998, Pages75-81);
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725 (for example APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714);
Described Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282 (for example CN102433277A).
Bacterium of the present invention all can be bought and obtain from commercial sources such as CGMCC, CCTCC and US mode culture collection warehousings (ATCC).
The enlarged culturing of each bacterial classification of the present invention is the cellar culture mode of this area, and the training method of recording in also can reference literature obtains.
The main beneficial effect that the present invention obtains is as follows: the present invention adopts pure microorganism treating sewage, environment friendly and pollution-free, with low cost, reasonable compatibility between each bacterial classification in microbiobacterial agent, symbiosis is coordinated, antagonism not mutually, and its preparation method is easy, easy to implement the method, it is easy and simple to handle, is beneficial to suitability for industrialized production; Process operations method of the present invention is simple, has reduced the wasting of resources, and less input, has reduced the cost of disposing of sewage; Treatment process of the present invention has purified sewage effectively, and dephosphorization, denitrogenation remove simultaneously, has solved the contradiction that in traditional technology, dephosphorization and denitrification effect are difficult to take into account simultaneously.
Embodiment
By the mode that adopts specific embodiment, the present invention is further elaborated below, but it should not be construed the restriction to core initiative spirit of the present invention.
Embodiment 1
A method of utilizing microbial preparation to dispose of sewage, comprising:
The preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, then standing 6 hours, cryodrying at the most rearmounted 4 ℃, dry after water content be controlled at 6%, obtain; By particle diameter, to be 10 object bamboo charcoals and chitosan be mixed to get according to the mass ratio of 1: 1 described carrier; Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part: 10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps; The concentration of above-mentioned each raw material bacterium is all controlled at 1 * 10 8individual/ml.
Sewage pre-treatment: first (NH3-N is 198mg/L by sewage, sulfide 38 mg/L, P is 26 mg/L) through solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, again liquid is removed to solid throw out by circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm
Microbiological oxidation: the liquid through circular hole filtering net enters microbial reaction pond, regulating pH is 7, by every cubic metre of liquid, adds 10 grams of microbial preparations at every turn, adds every day 1 time, adds continuously one week, last standing 3 days, liquid is discharged.After testing, the clearance of ammonia nitrogen in sewage, sulphur and phosphor contaminant can reach respectively 98%, 98% and 99%.
Embodiment 2
A method of utilizing microbial preparation to dispose of sewage, comprising:
The preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, then standing 8 hours, cryodrying at the most rearmounted 4 ℃, dry after water content be controlled at 6%, obtain; By particle diameter, to be 10 object bamboo charcoals and chitosan be mixed to get according to the mass ratio of 1: 1 described carrier; Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part: 10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps; The concentration of above-mentioned each raw material bacterium is all controlled at 2 * 10 8individual/ml; Above-mentioned thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259; Above-mentioned Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107; Above-mentioned sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589; Above-mentioned bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC 27142; Above-mentioned Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725; Above-mentioned Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282;
Sewage pre-treatment: first by sewage (sewage sample is from industry park, development area river wastewater) process solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, again liquid is removed to solid throw out by circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm
Microbiological oxidation: the liquid through circular hole filtering net enters micronic dust thing reaction tank, regulating pH is 7, by every cubic metre of liquid, adds 10 grams of microbial preparations at every turn, adds every day 1 time, adds continuously one week, last standing 3 days, liquid is discharged.After testing, pollutant load comparison before and after the sewage of processing, in Table 1:
Table 1
Although, above with general explanation and embodiment, this case having been done to detailed explanation, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the modification done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.

Claims (1)

1. a method of utilizing microbial preparation to dispose of sewage, is characterized in that, described method comprises the steps:
1) preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, standing 6-8 hour then, cryodrying at the most rearmounted 4 ℃, dry after water content be controlled at 6%, obtain; Described mixed bacteria liquid is mixed by the raw material bacterium of following weight part: 10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps;
2) sewage pre-treatment: first, by sewage process solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, then liquid is removed to solid throw out by circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm;
3) 10 grams of the microbial preparations of microbiological oxidation: the liquid after the filtration of circular hole filtering net enters microbial reaction pond, and regulating pH is 7, adds step 1 by every cubic metre of liquid at every turn) preparing, add every day 1 time, add continuously one week, last standing 3 days, liquid is discharged;
By bamboo charcoal and chitosan, the mass ratio according to 1: 1 is mixed to get described carrier;
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacil1us denitrificans) ATCC25259;
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107;
Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589;
Described bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142;
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725;
Described Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282.
CN201310419226.0A 2013-09-13 2013-09-13 Method for treating sewage by using microbial preparation CN103449681B (en)

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CN104402174A (en) * 2014-11-28 2015-03-11 云南圣清环保科技有限公司 Deep treatment method of coal chemical industry wastewater
CN105331598B (en) * 2015-07-16 2018-05-22 裴桂良 A kind of biological agent for being used to handle cable duct ash flushing water
CN106587506B (en) * 2015-07-26 2019-07-05 湖北康创科技有限公司 A method of removal wastewater pollutants containing arsenic
CN105036471B (en) * 2015-07-26 2017-10-27 广东利诚检测技术有限公司 A kind of method for removing leaded wastewater pollutants
CN105174654B (en) * 2015-10-16 2018-08-21 中国科学院水生生物研究所 A method of being used for landscape water body deodorization
CN105907673B (en) * 2016-04-29 2018-02-06 广州市佳境水处理技术工程有限公司 A kind of method using microorganism formulation enhanced water treatment biochemical effects
CN106045228A (en) * 2016-08-03 2016-10-26 吴小慧 Purification method of high-phosphorus-content wastewater
CN106862260A (en) * 2017-03-10 2017-06-20 刘晨 A kind of biochemical process for administering Cadmium Pollution in Soils

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