CN105130100A - Method of removing pollutant from cadmium-containing sewage - Google Patents

Method of removing pollutant from cadmium-containing sewage Download PDF

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CN105130100A
CN105130100A CN201510441587.4A CN201510441587A CN105130100A CN 105130100 A CN105130100 A CN 105130100A CN 201510441587 A CN201510441587 A CN 201510441587A CN 105130100 A CN105130100 A CN 105130100A
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sewage
liquid
solid
cadmium
remove
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CN105130100B (en
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李娜
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Henan Provincial Geological Environment Planning & Designing Co Ltd
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Priority to CN201710061958.5A priority Critical patent/CN106517701A/en
Priority to CN201710061968.9A priority patent/CN106630494B/en
Priority to CN201710061959.XA priority patent/CN106630490A/en
Priority to CN201510441587.4A priority patent/CN105130100B/en
Priority to CN201710061960.2A priority patent/CN106542706B/en
Priority to CN201710061963.6A priority patent/CN106745817B/en
Priority to CN201710061962.1A priority patent/CN106630491B/en
Application filed by Individual filed Critical Individual
Priority to CN201611187983.XA priority patent/CN106746216B/en
Priority to CN201710061966.XA priority patent/CN106630492A/en
Priority to CN201710061971.0A priority patent/CN106630495B/en
Priority to CN201710061957.0A priority patent/CN106630489A/en
Priority to CN201710061945.8A priority patent/CN106630488B/en
Priority to CN201710061967.4A priority patent/CN106630493A/en
Priority to CN201710061964.0A priority patent/CN106517702B/en
Publication of CN105130100A publication Critical patent/CN105130100A/en
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Publication of CN105130100B publication Critical patent/CN105130100B/en
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    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F2001/007—Processes including a sedimentation step
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/101—Sulfur compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
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    • C02F2303/00—Specific treatment goals
    • C02F2303/24—Separation of coarse particles, e.g. by using sieves or screens

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Abstract

The invention relates to a method of removing pollutants from cadmium-containing sewage, wherein the method includes the steps of: (a) enabling the sewage to pass through a solid-liquid separator and performing solid-liquid separation to remove large solid granular substances; (b) enabling the sewage to enter into a sedimentation tank, performing sedimentation for 12 h, and enabling the sewage to pass through a circular-mesh filter screen to remove solid flocculates; (c) feeding the filtered sewage into a biological reaction tank, regulating the pH to 7, adding a composite microbial inoculant to the sewage by 10 g per cubic meter of the sewage by one time per day, wherein the microbial inoculants is added continuously for one week; and (d) allowing the sewage for stand for 3 days and discharging the water. The method can effectively remove pollutants containing ammonia nitrogen, sulfur and phosphorus and cadmium, is low in investment cost and has excellent application prospect.

Description

A kind of method removed containing cadmium wastewater pollutants
Technical field
The invention belongs to environmental technology field, be specifically related to a kind of method removing dirty water pollutant.
Background technology
Along with the raising of industrial development and people's living standard, industrial sewage amount and city domestic sewage amount are surged just with surprising rapidity, these sewage or or polluted the river that the mankind depend on for existence, river and lake, have formed one of reason of prestige association environment for human survival.
In order to meet the public's improving constantly environmental quality requirement, country has worked out more and more stricter emission standard to nitrogen, researchs and develops economy, denitrogenates emphasis and the focus that treatment technology has become Water Pollution Control Engineering area research efficiently.Although there are many methods effectively can remove ammonia, as physical method has reverse osmosis, distillation, soil irrigation; Chemical method has ion exchange method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatment, catalytic pyrolysis; Biological method has nitrated and algal culture, but physical method treatment effect is not good, than chemical method, biological method process waste water has the following advantages: 1) often kind of chemical article is all the product that specific aim is very strong, just likely lost efficacy when running into other chemical substances, and the removal of biotechnological formulation to pollutent has spectrum; 2) chemical products can temporarily eliminate some objectionable impurities and mask malodors, lacks the generation that can not stop objectionable impurities; 3), after using chemical products, have residual in water body, may secondary pollution be caused.Natural microbial contained by biotechnological formulation, not containing pathogenic bacterium and pathogenic agent, these microorganisms are under the katalysis of enzyme, with the organic nutrient substance in sewage for food, after sewage is purified, these microbes reduce gradually with the reduction of pollutent, until wither away; 4) nontoxic, non-corrosiveness, easy to use, substantially do not need interpolation equipment or engineering, save fund input.
Sewage mainly contains sanitary sewage and trade effluent.Industrial sewage comparison of ingredients is complicated, particularly a large amount of artificial-synthetic compound's entered environments, this kind of material is ammonia nitrogen class, sulfide and P contained compound mainly, due to the complicacy of these these body structures of material, utilization can not be decomposed by the microorganisms at short notice, the microorganism of traditional method of wastewater treatment activated sludge culture can not be removed these pollutents effectively, these materials accumulate for a long time in the environment, the ecotope of depending on for existence to us causes very large pollution, brings very large harm to the physical and mental health of the mankind.Quite a few industrial pollution enterprise of China would rather be punished also to be unwilling to invest and administer waste water, even if there have waste disposal plant to run to be also abnormal.Therefore, develop that a kind of construction investment is few, running cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Summary of the invention
In order to overcome the deficiencies in the prior art, the pollutents such as the ammonia nitrogen sulphur phosphorus effectively simply in removal sewage and heavy metal, the invention provides a kind of method removing dirty water pollutant, its technical scheme realizes by the following method:
Remove the method containing cadmium wastewater pollutants, it comprises the steps:
The preparation of composite fungus agent: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stir, then leaves standstill 6 hours, cryodrying under the most rearmounted 4 ° of C, and after dry, water content control is 6%, to obtain final product; Described carrier is mixed to get by bamboo charcoal, chitosan and the diatomite mass ratio according to 2:2:1, particle diameter preferably 10 orders of bamboo charcoal;
Sewage treatment step: first by sewage through solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, again liquid is removed solid floccules by circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm, bio-oxidation: the liquid through circular hole filtering net enters biological reaction tank, regulates pH to be 7, adds composite fungus agent 10 grams by every cubic metre of liquid at every turn, add 1 every day, add one week continuously, finally leave standstill 3 days, liquid is discharged.
Preferably, described composite fungus agent is mixed by the raw material bacterium of following weight part:
Rhodococcus 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts, bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts; The concentration of above-mentioned each raw material bacterium all controls in (1-2) × 10 8individual/ml.Above-mentioned bacterial classification can be the conventional bacterial strain of prior art;
Preferably,
Described rhodococcus is rhodococcus (Rhodococcusrhodochrous) ATCC15906;
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillusdenitrificans) ATCC25259;
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonasstutzeri) CCTCCNO:M209107;
Described Sphingomonas is Sphingomonas (Sphingomonassp.) CGMCCN0.4589;
Described bacillus pumilus is bacillus pumilus (Bacilluspumilus) ATCC27142;
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaetechrysosporium) ATCC24725.
Bacterium of the present invention all can be bought from commercial sources such as CGMCC, CCTCC and American Type Culture collection warehousings (ATCC) and obtain.
Preferably, described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaetechrysosporium) the ATCC24725 bacterial strain of the encoding gene having proceeded to coding any one protein variant following, described protein variant is suddenly change as follows respectively relative to albumen original amino acid 71S/T, 89T/Y, 121T/A, 143D/S, 184S/Q, 239W/G, 246V/P, 269K/N, 304P/S, 353K/E, 369G/D, 372C/S, 422D/R, 448G/I, and albumen original amino acid is see GenBank:NP_744955.1.
The present invention provide simultaneously a kind of can the transgenic strain of efficient absorption cadmium, this bacterial strain realizes its object by the sequence of aminoacid sequence as shown in Genbank:NP_744955.1 being imported in Phanerochaete chrysosporium.
And described aminoacid sequence can have the sudden change in sites different as follows, 71S/T, 89T/Y, 121T/A, 143D/S, 184S/Q, 239W/G, 246V/P, 269K/N, 304P/S, 353K/E, 369G/D, 372C/S, 422D/R, 448G/I.(71S/T represent replace with T amino acid at the S amino acid of original series the 71st).The bacterial strain that aminoacid sequence after these sudden changes is not willing the aminoacid sequence after having imported this sudden change has similar effect equally.And applicant is confirmed by a large amount of experiments, not all replacement all has similar effect, has the Phanerochaete chrysosporium after a large amount of replacements not have the effect of good sewage disposal together with other bacterial strain.
Utilize DNAMAN software design primer, add BamHI and SalI restriction enzyme site respectively, nucleotide sequence HP gene according to the synthesis of aminoacid sequence NP_744955.1 full genome, object fragment is obtained by carrying out amplification, pcr amplification obtains goal gene HP(and is incorporated in gene order by multiplex PCR by corresponding mutational site simultaneously, thus obtain different mutants gene), double digestion PCR primer is carried out with BamHI and SalI, be connected with the same expression vector PWB980 through BamHI with SalI double digestion, the recombinant plasmid transformed be proved to be successful is entered into described Phanerochaete chrysosporium, obtain the genetic engineering bacterium of degraded cadmium.
The enlarged culturing of each bacterial classification of the present invention is the cellar culture mode of this area, and the training method also can recorded in reference literature obtains.
The main beneficial effect that the present invention obtains is as follows: the present invention adopts pure microbial inoculum to dispose of sewage, effectively purify sewage, and dephosphorization, denitrogenation are removed simultaneously, the heavy metal cadmium simultaneously can removing sewage Central China on this basis solves the contradiction that dephosphorization and denitrification effect in traditional technology are difficult to take into account heavy metals removal simultaneously, has good application prospect.
Embodiment
Embodiment 1
Remove a method for dirty water pollutant, it comprises the steps:
The preparation of composite fungus agent: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stir, then leaves standstill 6 hours, cryodrying under the most rearmounted 4 ° of C, and after dry, water content control is 6%, to obtain final product; Carrier is mixed to get by bamboo charcoal, chitosan and the diatomite mass ratio according to 2:2:1; Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part: rhodococcus 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts, bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts; The concentration of above-mentioned each raw material bacterium all controls 1 × 10 8individual/ml.
Sewage disinfection treatment: first (NH3-N is 300mg/L by sewage, sulfide 80mg/L, be 70mg/L containing phosphor contaminant, cadmium content is 40mg/ml, through solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitates 12 hours, again liquid is removed solid floccules by circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm;
Bio-oxidation: the liquid through circular hole filtering net enters biological reaction tank, regulate pH to be 7.0, add composite fungus agent 10 grams by every cubic metre of liquid, every day adds I time at every turn, adds one week continuously, finally leaves standstill 3 days, is discharged by liquid.After testing, the content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, and be 2.5mg/L containing phosphor contaminant, clearance all reaches more than 95%; And cadmium content is 37mg/mL, removal effect is not remarkable.
Embodiment 2
Utilize DNAMAN software design primer, add BamHI and SalI restriction enzyme site respectively, nucleotide sequence HP gene according to the synthesis of aminoacid sequence NP_744955.1 full genome, object fragment is obtained by carrying out amplification, pcr amplification obtain goal gene HP(simultaneously by multiplex PCR by corresponding mutational site 71S/T, 89T/Y, 121T/A, 143D/S, 184S/Q, 239W/G, 246V/P, 269K/N, 304P/S, 353K/E, 369G/D, 372C/S, 422D/R, 448G/I is incorporated in gene order, thus obtain different mutants gene), double digestion PCR primer is carried out with BamHI and SalI, be connected with the same cloning vector PWB980 through BamHI with SalI double digestion, the recombinant plasmid transformed be proved to be successful is entered into described Phanerochaete chrysosporium (Phanerochaetechrysosporium) ATCC24725, obtain the genetic engineering bacterium of degraded cadmium.
The wastewater treatment efficiency of embodiment 3 Phanerochaete chrysosporium genetic engineering bacterium and all the other several microbial inoculums is verified
According to the method for embodiment 1, carry out corresponding sewage disposal experiment, the sewage of its sewage and embodiment 1 belongs to same batch, has the Pollutant levels of same concentrations.Wherein each component composition of microbial inoculum and the identical of embodiment 1.
The genetic engineering bacterium in the different mutational site adopting embodiment 2 to obtain respectively carries out removing the reaction of cadmium, found through experiments, this several mutant bacteria of 71S/T, 89T/Y, 121T/A, 143D/S, 184S/Q, 239W/G, 246V/P, 269K/N, 304P/S, 353K/E, 369G/D, 372C/S, 422D/R, 448G/I all has and has relative to original bacteria the effect obviously strengthened.Be not more than 4 days overall treatment time, its result is as follows: sewage is with a collection of sewage, thus guaranteed conditions is consistent.
Each Pollutant levels of sewage: NH3-N is 300mg/L, sulfide 80mg/L, is 70mg/L containing phosphor contaminant, and cadmium content is 40mg/ml.
Strain name NH3-N residual concentration (mg/L) Sulfide residual concentration (mg/L) Containing phosphor contaminant residual concentration (mg/L) Cadmium residual concentration (mg/L)
Phanerochaete chrysosporium original strain 12.5 4.5 2.5 37
HBY5-（71S/T） 1.1 0.8 0.7 0.2
HBY7-（89T/Y） 1.2 0.7 0.7 0.3
HBY8-（121T/A） 1.3 0.8 0.6 0.1
HBY10-（143D/S） 1.1 0.9 0.6 0.2
HBY12-（184S/Q） 1.0 0.7 0.5 0.3
HBY13-（239W/G） 1.1 0.6 0.6 0.3
HBY14-（246V/P） 1.3 0.8 0.6 0.2
HBY19-（269K/N） 1.0 0.9 0.7 0.4
HBY22-（304P/S） 1.4 0.7 0.8 0.3
HBY26-（353K/E） 0.9 0.6 0.8 0.2
HBY28-（369G/D） 1.0 0.8 0.6 0.3
HBY33-（372C/S） 1.3 0.8 0.7 0.2
HBY40-（422D/R） 1.0 0.7 0.7 0.5
HBY45-（448G/I） 0.9 0.9 0.8 0.1
As can be seen from the above results, the microbial inoculum that the Phanerochaete chrysosporium adopting genetically engineered to build and other several bacterial strains are formed is except having the effect of original good removal ammonia nitrogen, sulfide and phosphorus, also there is the effect removing cadmium preferably, and removing the synergy also between engineered Phanerochaete chrysosporium and other several bacterial strains in ammonia nitrogen, sulfide and phosphorus with enhancing.

Claims (1)

1. remove the method containing cadmium wastewater pollutants for one kind, its comprise the steps: first by sewage through solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, again liquid is removed solid floccules by circular hole filtering net, liquid through circular hole filter screen filtration enters biological reaction tank, regulates PH to be 7, adds composite fungus agent 10 grams by every cubic metre of liquid at every turn, add 1 every day, add one week continuously, finally leave standstill 3 days, liquid is discharged.
CN201510441587.4A 2015-07-26 2015-07-26 It is a kind of to remove the method containing cadmium wastewater pollutants Expired - Fee Related CN105130100B (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
CN201710061959.XA CN106630490A (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201510441587.4A CN105130100B (en) 2015-07-26 2015-07-26 It is a kind of to remove the method containing cadmium wastewater pollutants
CN201710061960.2A CN106542706B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061963.6A CN106745817B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061962.1A CN106630491B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061971.0A CN106630495B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201611187983.XA CN106746216B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061966.XA CN106630492A (en) 2015-07-26 2015-07-26 Method for removing pollutant in cadmium-containing sewage
CN201710061958.5A CN106517701A (en) 2015-07-26 2015-07-26 Method for removing pollutants in cadmium-containing sewage
CN201710061957.0A CN106630489A (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061945.8A CN106630488B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061968.9A CN106630494B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061964.0A CN106517702B (en) 2015-07-26 2015-07-26 Method for removing cadmium-containing sewage pollutants
CN201710061967.4A CN106630493A (en) 2015-07-26 2015-07-26 Method for removing contaminants from cadmium-containing sewage

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Application Number Priority Date Filing Date Title
CN201510441587.4A CN105130100B (en) 2015-07-26 2015-07-26 It is a kind of to remove the method containing cadmium wastewater pollutants

Related Child Applications (13)

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