CN108529760B - Microbial remediation method for coupling easily-degradable organic wastewater and heavy metal polluted wastewater - Google Patents
Microbial remediation method for coupling easily-degradable organic wastewater and heavy metal polluted wastewater Download PDFInfo
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- CN108529760B CN108529760B CN201810383232.8A CN201810383232A CN108529760B CN 108529760 B CN108529760 B CN 108529760B CN 201810383232 A CN201810383232 A CN 201810383232A CN 108529760 B CN108529760 B CN 108529760B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
Abstract
A microorganism remediation method for coupling easily degradable organic wastewater and heavy metal polluted wastewater relates to a method for treating heavy metal polluted wastewater by adopting organic wastewater and microorganism adsorption, and belongs to the technical field of environmental protection. The invention aims to solve the technical problem that the cost of the external carbon source is overhigh in the existing method. The method comprises the following steps: mixing easily degradable organic wastewater and heavy metal polluted wastewater, adding a sewage treatment microbial inoculum, placing a filler at the bottom of a water body, placing a suspended filler, taking out the filler and the suspended filler at the bottom of the water body, and removing surface microbial residues until the chromium content in the water body is less than 1.5mg/L and the lead content is less than 0.5mg/L, thereby completing the process. According to the method, organic nutrients in the organic wastewater are utilized, the determination of key indexes is combined, the carbon source in the heavy metal wastewater is limited to be an organic wastewater adding node, an organic carbon source is provided for heavy metal adsorption microorganisms, the heavy metal is effectively adsorbed, the utilization rate of the organic wastewater is improved, and the purpose of treating wastes with processes of wastes against one another is achieved.
Description
Technical Field
The invention relates to a method for treating heavy metal polluted wastewater by adopting organic wastewater and microorganism adsorption, belonging to the technical field of environmental protection.
Background
Heavy metal pollution of a water body is one of common pollution, and countries in the world aim to seek a sewage treatment technology with low energy consumption, low cost and small secondary pollution. Heavy metals are widely available and mainly originate from waste water of fuel combustion, mining metallurgy, petroleum refining, chemical production and other industries. The industrial development leads to the excessive heavy metal in local water areas, which causes serious pollution to the ecological environment and seriously damages human health. The microorganisms have certain adaptability to heavy metal ions, and convert toxic metal ions into low-toxicity or non-toxic precipitates in an oxidation reduction mode through self metabolism and metabolites thereof, so that the harmless treatment of the heavy metal wastewater is realized. The adsorption of heavy metals by microorganisms mainly exists in two forms, namely active adsorption and passive adsorption. A large number of researches show that the microbial adsorption of heavy metals in wastewater is mainly passive adsorption. It is worth noting that the adsorption of the heavy metal by the microorganism is irreversible, and the heavy metal ions form inorganic micro-precipitates in cells and precipitate on the cell surfaces or in the cells, so that the secondary pollution of the heavy metal is reduced as much as possible.
In the traditional heavy metal sewage treatment, microorganisms are generally directly inoculated in the sewage, and microbial aerosol is formed under the action of aeration or mechanical agitation, so that certain pollution is caused to the surrounding environment. The strain is not easy to fix, easy to run off and slow to recover. Along with the metabolism of microorganisms in a water body, the type and the content of a carbon source directly influence the dephosphorization effect in the sewage treatment process, and in addition, the shortage of the carbon source becomes an important factor for limiting the sewage treatment quality. However, the cost of the added carbon source is too high, so most of the research is mainly focused on the optimization of the sewage treatment system and the utilization rate of the carbon source. However, waste water from food processing, paper industry, sugar industry, etc. contains a large amount of organic substances such as carbohydrates, fats, proteins, lignin, etc. If the organic wastewater is combined with the nutrient-limited heavy metal wastewater, the problem of carbon source limitation in the heavy metal wastewater treatment process can be effectively solved, the utilization of eutrophic wastewater can be effectively enhanced, and the purpose of treating waste by waste is achieved.
Disclosure of Invention
The invention aims to solve the technical problem of overhigh cost of an external carbon source in the existing method, and provides a microbial remediation method for coupling easily-degradable organic wastewater and heavy metal polluted wastewater.
The microbial remediation method for coupling the easily degradable organic wastewater and the heavy metal polluted wastewater comprises the following steps:
firstly, respectively selecting two polluted water bodies of easily degradable organic wastewater and heavy metal polluted wastewater;
secondly, mixing the easily degradable organic wastewater and the heavy metal polluted wastewater until the BOD content is 20-50mg/L, COD content is 50-100mg/L and the heavy metal content is 50-100mg/L in the water body, and adding a sewage treatment microbial inoculum, wherein the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1: 1000-;
thirdly, according to the area of the reservoir, placing filler at the bottom of the water body obtained in the second step according to the adding proportion of 2-5kg of solid filler in each square meter;
fourthly, placing a suspension type filler in the water body before the number of the microorganisms stops growing, namely the OD value of the microorganisms in the logarithmic growth phase is 0.6-0.8;
or when the organic carbon source in the wastewater becomes a limiting factor, namely the TOC concentration of the water body is lower than 20mg/L, placing a suspended filler in the water body;
placing the suspended filler in the water body until the microbial growth on the surface of the suspended filler reaches a peak value, taking out the filler and the suspended filler at the bottom of the water body, and removing microbial residues on the surface;
sixthly, according to the concentration of the heavy metals in the water body, the concentration of the heavy metals does not meet the pollutant discharge standard GB20426-2006, when the TOC concentration in the water body is lower than 20mg/L, organic wastewater is supplemented until the TOC concentration in the water body is 50-100mg/L, a sewage treatment microbial inoculum is added, the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1:1000 plus 5000, the steps from three to five are repeated until the total chromium content in the water body is less than 1.5mg/L and the lead content in the water body is less than 0.5mg/L, and then the microbial remediation of the coupling of the easily degradable organic wastewater and the heavy metal polluted wastewater is completed.
The easily degradable organic wastewater in the step one is the wastewater of processing aquatic products, meat and fresh vegetables; wastewater from dessert, beverage, and dairy food manufacturing; brewing and fermenting industrial wastewater.
The heavy metal polluted wastewater in the step one is industrial wastewater prepared from coal, metallurgy, electroplating, tanning and a catalyst.
The sewage treatment microbial inoculum in the step two is nitrobacteria, Klebsiella, fungus phoenix mushroom or Phanerochaete chrysosporium.
And in the third step, the filler is sugar-making industrial waste residue, food manufacturing workshop waste residue, brewing workshop industrial waste residue and fermentation workshop industrial waste residue.
The suspended filler in the fourth step is sugar-making industrial waste residue, food manufacturing workshop waste residue, brewing workshop industrial waste residue and fermentation workshop industrial waste residue.
The microbial number in the fourth step is measured by adopting plate counting, photoelectric turbidimetry and/or fluorescent quantitative PCR, and the organic carbon source limiting factor is subjected to correlation analysis (Pearson analysis or redundancy analysis) by a statistical method.
And placing the suspension type filler in the water body for 5-9 days in the step five.
The microbial agent in the sixth step is nitrobacteria, Klebsiella, Pleurotus sajor-caju or Phanerochaete chrysosporium.
And step six, determining the concentration of the heavy metal in the water body by adopting an atomic absorption method, an ultraviolet-visible spectrophotometry method or a liquid chromatography-mass spectrometry method.
The invention provides a method for treating heavy metal wastewater by using microorganisms, which is different from the traditional method for treating the heavy metal wastewater by using organic nutrients in the organic wastewater, combines the determination of key indexes, limits a carbon source in the heavy metal wastewater to be an organic wastewater adding node, provides an organic carbon source for heavy metal adsorption microorganisms, effectively adsorbs heavy metals, improves the utilization rate of the organic wastewater and achieves the aim of treating the wastewater by using waste.
The method has strong pertinence and accuracy, improves the utilization rate of the organic wastewater, and enhances the treatment of the heavy metal polluted wastewater.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the microorganism repairing method for coupling the easily degradable organic wastewater and the heavy metal polluted wastewater comprises the following steps:
firstly, respectively selecting two polluted water bodies of easily degradable organic wastewater and heavy metal polluted wastewater;
secondly, mixing the easily degradable organic wastewater and the heavy metal polluted wastewater until the BOD content is 20-50mg/L, COD content is 50-100mg/L and the heavy metal content is 50-100mg/L in the water body, and adding a sewage treatment microbial inoculum, wherein the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1: 1000-;
thirdly, according to the area of the reservoir, placing filler at the bottom of the water body obtained in the second step according to the adding proportion of 2-5kg of solid filler in each square meter;
fourthly, placing a suspension type filler in the water body before the number of the microorganisms stops growing, namely the OD value of the microorganisms in the logarithmic growth phase is 0.6-0.8;
or when the organic carbon source in the wastewater becomes a limiting factor, namely the TOC concentration of the water body is lower than 20mg/L, placing a suspended filler in the water body;
placing the suspended filler in the water body until the microbial growth on the surface of the suspended filler reaches a peak value, taking out the filler and the suspended filler at the bottom of the water body, and removing microbial residues on the surface;
sixthly, according to the concentration of the heavy metals in the water body, the concentration of the heavy metals does not meet the pollutant discharge standard GB20426-2006, when the TOC concentration in the water body is lower than 20mg/L, organic wastewater is supplemented until the TOC concentration in the water body is 50-100mg/L, a sewage treatment microbial inoculum is added, the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1:1000 plus 5000, the steps from three to five are repeated until the total chromium content in the water body is less than 1.5mg/L and the lead content in the water body is less than 0.5mg/L, and then the microbial remediation of the coupling of the easily degradable organic wastewater and the heavy metal polluted wastewater is completed.
The second embodiment is as follows: the difference between the first embodiment and the second embodiment is that the easily degradable organic wastewater in the first step is aquatic product, meat and vegetable fresh food processing wastewater; wastewater from dessert, beverage, and dairy food manufacturing; brewing and fermenting industrial wastewater. The rest is the same as the first embodiment.
The third concrete implementation mode: the embodiment is different from the first or second embodiment in that the heavy metal polluted wastewater in the first step is industrial wastewater prepared by coal, metallurgy, electroplating, tanning and catalysts. The other is the same as in one or both of the first and second embodiments.
The fourth concrete implementation mode: the present embodiment is different from the first to the third embodiments in that the microbial agent in the second step is nitrobacteria, klebsiella, phoenix mushroom or phanerochaete chrysosporium. The others are the same as in one of the first to third embodiments.
The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is that the filler in step three is sugar manufacturing industrial waste residue, food manufacturing plant waste residue, brewing plant industrial waste residue, and fermentation plant industrial waste residue. The other is the same as one of the first to fourth embodiments.
The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is that the suspended filler in step four is sugar manufacturing industrial waste residue, food manufacturing plant waste residue, brewing plant industrial waste residue, and fermentation plant industrial waste residue. The other is the same as one of the first to fifth embodiments.
The seventh embodiment: the difference between this embodiment and the first to the sixth embodiment is that the number of microorganisms in the fourth step is measured by plate counting, photoelectric turbidimetric and/or fluorescent quantitative PCR, and the organic carbon source limiting factor is statistically analyzed. The other is the same as one of the first to sixth embodiments.
The specific implementation mode is eight: the difference between the first embodiment and the seventh embodiment is that the suspension filler is placed in the water body for 5-9 days in the fifth embodiment. The other is the same as one of the first to seventh embodiments.
The specific implementation method nine: the present embodiment is different from the first to eighth embodiments in that the microbial agent in the sixth step is nitrobacteria, klebsiella, phoenix mushroom, or phanerochaete chrysosporium. The rest is the same as the first to eighth embodiments.
The detailed implementation mode is ten: the present embodiment differs from one of the first to ninth embodiments in that the concentration of heavy metals in the water in the sixth step is measured by an atomic absorption method, an ultraviolet-visible spectrophotometry method or a liquid chromatography-mass spectrometry method. The other is the same as one of the first to ninth embodiments.
The following experiments are adopted to verify the effect of the invention:
experiment one:
the microbial remediation method for coupling the easily degradable organic wastewater and the heavy metal polluted wastewater comprises the following steps:
firstly, respectively selecting two polluted water bodies of easily degradable organic wastewater and heavy metal polluted wastewater;
mixing the easily degradable organic wastewater and the heavy metal polluted wastewater until the BOD content is 50mg/L, COD content and the heavy metal content is 50mg/L in the water body, and adding a sewage treatment microbial inoculum, wherein the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1: 1000;
thirdly, according to the area of the reservoir, according to the adding proportion of 2kg of solid filler added per square meter, filling materials (sugar manufacturing industrial waste residues) are placed at the bottom of the water body obtained in the second step;
fourthly, extracting the DNA of the water body, performing fluorescent quantitative PCR amplification by using a 16SrDNA universal primer, determining the number of microorganisms in the water body, wherein the number of bacteria in the water body is 7.8 multiplied by 10 according to a fluorescent quantitative result8copying/mL, placing a suspension type filler (sugar manufacturing industrial waste residue) in a water body;
fifthly, placing the suspended filler in the water body for 5 days, extracting the filler DNA, and carrying out the fluorescence quantitative PCR experiment in the same way, wherein the number of bacteria in the filler is 1.5 multiplied by 1010Copying, taking out the filler and the suspended filler at the bottom of the water body, and removing microbial residues on the surface;
and sixthly, taking the treated water body, and measuring the concentration of the heavy metal by an atomic absorption method, wherein the concentration of lead is 0.32mg/L, and the concentration of chromium is 0.62mg/L, so that the treatment standard is met, and the microbial remediation of the coupling of the easily degradable organic wastewater and the heavy metal polluted wastewater is completed.
Claims (6)
1. The microbial remediation method for coupling the easily degradable organic wastewater with the heavy metal polluted wastewater is characterized by comprising the following steps of:
firstly, respectively selecting two polluted water bodies of easily degradable organic wastewater and heavy metal polluted wastewater;
secondly, mixing the easily degradable organic wastewater and the heavy metal polluted wastewater until the BOD content is 20-50mg/L, COD content is 50-100mg/L and the heavy metal content is 50-100mg/L in the water body, and adding a sewage treatment microbial inoculum, wherein the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1: 1000-;
thirdly, according to the area of the reservoir, placing filler at the bottom of the water body obtained in the second step according to the adding proportion of 2-5kg of solid filler in each square meter;
fourthly, placing a suspension type filler in the water body before the number of the microorganisms stops growing, namely the OD value of the microorganisms in the logarithmic growth phase is 0.6-0.8;
or when the organic carbon source in the wastewater becomes a limiting factor, namely the TOC concentration of the water body is lower than 20mg/L, placing a suspended filler in the water body;
placing the suspended filler in the water body until the microbial growth on the surface of the suspended filler reaches a peak value, taking out the filler and the suspended filler at the bottom of the water body, and removing microbial residues on the surface;
sixthly, according to the concentration of heavy metals in the water body, the concentration of the heavy metals does not meet the pollutant discharge standard GB20426-2006, when the TOC concentration in the water body is lower than 20mg/L, organic wastewater is supplemented until the TOC concentration in the water body is 50-100mg/L, a sewage treatment microbial inoculum is added, the volume ratio of the sewage treatment microbial inoculum to the water body to be treated is 1:1000 plus 5000, the steps from three to five are repeated until the total chromium content in the water body is less than 1.5mg/L and the lead content is less than 0.5mg/L, and then the microbial remediation of the coupling of the easily degradable organic wastewater and the heavy metal polluted wastewater is completed;
the filler in the third step is sugar-making industrial waste residue, food manufacturing workshop waste residue, brewing workshop industrial waste residue and fermentation workshop industrial waste residue;
the suspension type filler in the step four is sugar manufacturing industrial waste residue, food manufacturing workshop waste residue, brewing workshop industrial waste residue and fermentation workshop industrial waste residue;
the sewage treatment microbial inoculum in the step two is nitrobacteria, Klebsiella, fungus phoenix mushroom or Phanerochaete chrysosporium; the microbial agent in the sixth step is nitrobacteria, Klebsiella, Pleurotus sajor-caju or Phanerochaete chrysosporium.
2. The method for microbial remediation of coupling easily degradable organic wastewater with wastewater polluted by heavy metals according to claim 1, wherein the easily degradable organic wastewater in the step one is wastewater from fresh food processing of aquatic products, meat and vegetables; wastewater from dessert, beverage, and dairy food manufacturing; brewing and fermenting industrial wastewater.
3. The method for microbial remediation of easily degradable organic wastewater coupled with heavy metal contaminated wastewater according to claim 1, wherein the heavy metal contaminated wastewater in the step one is industrial wastewater prepared from coal, metallurgy, electroplating, tanning and catalysts.
4. The microbial remediation method of claim 1, wherein the amount of microorganisms in the fourth step is determined by plate counting, photoelectric turbidimetry and/or fluorescent quantitative PCR, and the organic carbon source limiting factor is analyzed by statistical methods for correlation.
5. The microbial remediation method of claim 1, wherein in step five, the suspended filler is placed in the water for 5-9 days.
6. The microbial remediation method of claim 1, wherein the concentration of heavy metals in the sixth step of water is determined by atomic absorption, ultraviolet-visible spectrophotometry or liquid chromatography-mass spectrometry.
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| CN115710554B (en) * | 2022-12-28 | 2024-01-30 | 吉林农业大学 | Pleurotus pulmonarius strain for decoloring and removing COD (chemical oxygen demand) in sewage and application thereof |
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| CN1978639A (en) * | 2005-12-02 | 2007-06-13 | 南京理工大学 | Method for preparing immobilized thallus and its use |
| CN101974471A (en) * | 2010-11-12 | 2011-02-16 | 东华大学 | Sphingosine monad DX-T3-03 strain and extracting method thereof |
| CN105130100A (en) * | 2015-07-26 | 2015-12-09 | 李娜 | Method of removing pollutant from cadmium-containing sewage |
| CN107352737A (en) * | 2017-07-17 | 2017-11-17 | 湖北三里枫香科技有限公司 | A kind of bioanalysis metal recovery processes |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1978639A (en) * | 2005-12-02 | 2007-06-13 | 南京理工大学 | Method for preparing immobilized thallus and its use |
| CN101974471A (en) * | 2010-11-12 | 2011-02-16 | 东华大学 | Sphingosine monad DX-T3-03 strain and extracting method thereof |
| CN105130100A (en) * | 2015-07-26 | 2015-12-09 | 李娜 | Method of removing pollutant from cadmium-containing sewage |
| CN107352737A (en) * | 2017-07-17 | 2017-11-17 | 湖北三里枫香科技有限公司 | A kind of bioanalysis metal recovery processes |
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