CN101974471A - Sphingosine monad DX-T3-03 strain and extracting method thereof - Google Patents
Sphingosine monad DX-T3-03 strain and extracting method thereof Download PDFInfo
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Abstract
The invention relates to a sphingosine monad DX-T3-03 strain and an extracting method thereof. The sphingosine monad DX-T3-03 strain has high resistance against zinc and phenol degradation capability, is separated and extracted from the soil of a tailing dam of a mine, achieves high tolerance to the zinc at 4 mM/L and high degradation rate of phenol at 88 percent, has low requirements on nutrition and important application potential in biologically treating double pollution of phenol-containing industrial waste water and heavy metals; in addition, the extracting method is simple, convenient, fast, low in cost and environmental-friendly.
Description
Technical field
The invention belongs to Sphingol single-cell bacterial strain field, particularly a kind of Sphingol single-cell DX-T3-03 bacterial strain and extracting method thereof.
Background technology
Along with the development of mining industry and heavy metal electroplate, the application of aspects such as metallurgical, chemical industry and pharmacy industry, heavy metal contamination is on the rise.Because characteristics such as heavy metal difficult degradation in soil, difficult migration, harm phase length have made that heavy metal pollution of soil becomes one of environmental problem of domestic and international common concern.Wherein heavy metal zinc is important nutritive element, also is pollution element.Zinc once was described as " flower of life ", was to keep the growth of body normal growth, metabolic important substance.China produces zinc big country, and maximum consumer field is zinc-plated industry, and consumption accounts for 30%40% of zinc ultimate production.In addition, the zinc-containing water of other industry generation also can not be ignored.Many experiments and epidemiology survey are verified, if zinc is too high at people's in-vivo content, will suppress cytophagous activity and sterilizing power, thereby reduce the immunologic function of human body, and disease susceptibility is increased.Nearest scientific research shows, the zinc of excess intake can cause certain injury to human body, even can cause poisoning, so that dead; Zinc poisoning can cause multicomponent system (Muitisystem) dysfunction even death in pancreas, kidney and the body simultaneously.
For the processing of heavy metal, because traditional physics or chemical process often follow secondary pollution, and the working cost height, complicated operation more and more is not suitable for the needs of modern Pollution abatement.In recent years, the potential application foreground that microorganism is administered in heavy metal contamination makes it become a domestic and international novel research direction on the processing heavy metal pollution problem.Therefore, new microorganism with high resistance capacity of screening and the resistance mechanism of studying them also become one of current main task.
Phenol is a kind of organic pollutant that produces in the commercial runs such as papermaking, oil refining, pharmacy, coking, can produce poisonous even fatal effect to animal and plant.Discharging and the accumulation deterioration day by day that caused ecotope in soil and water body of a large amount of aldehydes matters and derivative thereof, many countries are listed it in the Black List of environment priority pollutants.In recent years, along with the development of biological new technology, utilize microbial degradation method to handle high-concentration phenolic wastewater and be a kind of economical and efficient and can not produce the method for secondary pollution that the research of related fields more and more is subjected to people's attention.But contain the heavy metal ion that often contains higher concentration in the phenol trade effluent, and the existence of high density heavy metal ion can suppress microorganism Pyrogentisinic Acid's biological degradation.Therefore, obtain have the phenol degrading bacterium of certain tolerance heavy metal ability in the biological treatment that contains the phenol trade effluent, will have outstanding application potential.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Sphingol single-cell DX-T3-03 bacterial strain and extracting method thereof, this bacterial strain is to the tolerance of zinc up to 4mM/L the time, the degradation rate of phenol is up to 88%, nutritional requirement is not high, the Pyrogentisinic Acid has degradation capability preferably, contains in phenol trade effluent and the dual pollution of heavy metal at biological treating to have the important application potentiality; Simple and convenient extraction is quick, and cost is low, and is environmentally friendly.
A kind of Sphingol single-cell DX-T3-03 bacterial strain of the present invention is characterized in that: this bacterial strain has high resistance and has the degradation of phenol ability zinc.
The extracting method of a kind of Sphingol single-cell DX-T3-03 bacterial strain of the present invention comprises:
(1) directed domestication
Fetch earth in the mining area and to make soil supension and be inoculated in the minimal medium, with gradient pressure type method for domesticating domestication 2 months by the access amount of 5wt%;
(2) concentration and separation of bacterial strain and purifying
Sample after the above-mentioned domestication is left standstill, get the upper strata water sample and make gradient dilution, every kind of weaker concn is got 1mL bacterium liquid respectively and is carried out enrichment culture in being inoculated into solid medium then, and so repeated multiple times guarantees to grow pure single bacterial strain, inoculate slant medium, get final product.
Minimal medium in the described step (1) is (NH
4)
2SO
410.0g, KH
2PO
47.5g, Na
2HPO
410.0g, FeCl
30.0125g, MgSO
47H
2O 0.015g, distilled water 1000mL, pH=6.5～7.0.
Gradient pressure type method for domesticating in the described step (1) is in minimal medium, adds the bacteriological filtration phenol and the bacteriological filtration Zn of gradient concentration simultaneously
2+Solution makes and contains phenol and Zn in the substratum simultaneously
2+And two kinds of material concentrations all raise successively, i.e. phenol 300,400,500,600,700,800,900,1000mg/L, Zn
2+Concentration 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0mM/L, be 6～7d the pitch time that changes concentration, in 30～35 ℃, tames cultivation under the oscillation rate 150r/min condition.
Gradient dilution in the described step (2) is 10
-1～10
-10Gradient dilution.
Solid medium in the described step (2) is the agar that adds massfraction 1.5% on the enrichment liquid medium base, 1 * 10
5The Pa 30min that sterilizes, cooling back and the substratum that forms, wherein the enrichment liquid nutrient medium is peptone 0.25g, Tryptones 0.25g, yeast powder 0.5g, glucose 0.5g, MgSO
47H
2O 0.03g, distilled water 1000mL, pH=6.5～7.0.Slant medium is got clean test tube for solid medium heating is dissolved, and pours about 5mL solid medium in the test tube into, will try nose end to be placed on the glass stick, makes pipe interior substratum formation inclined-plane and forms.
Enrichment culture is meant bacterium liquid is cultivated 48h after being inoculated into enrichment medium in 30 ℃ of incubator in the described step (2), the single clearly bacterium colony of picking form, numbering, on solid medium, rule, culture dish is inverted in 30 ℃ of incubators cultivates 48h, observations is observed colonial morphology under electron microscope again, 2～4 times repeatedly, guarantee to grow pure single bacterial strain.
The 16S rDNA sequence of Sphingol single-cell DX-T3-03 bacterial strain is as follows:
CCAATGGCGGCTGCCTACACATGCAGTCGAACGAGATCTTCGGGTCTAGTGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTTTGGTTCGGAATAACAGTTGGAAACGACTGCTAATACCGGATGATGACGAAAGTCCAAAGATTTATCGCCAGAGGATGAGCCCGCGTAGGATTAGCTAGTTGGTGTGGTAAAGGCGCACCAAGGCGACGATCCTTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTTACCCGGGATGATAATGACAGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTTTGTAAGTTAGAGGTGAAAGCCTGGAGCTCAACTCCAGAACTGCCTTTAAGACTGCATCGCTTGAATCCAGGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGGCTCACTGGACTGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAACTAGCTGTCCGGGGACTTGGTCTTTGGGTGGCGCAGCTAACGCATTAAGTTATCCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCTGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCGTTTGACATGTCCGGACGATTTCCAGAGATGGATCTCTTCCCTTCGGGGACTGGAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCTTTAGTTACCATCATTTAGTTGGGGACTCTAAAGGAACCGCCGGTGATAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTACGCGCTGGGCTACACACGTGCTACAATGGCGGTGACAGTGGGCAGCAAACTCGCGAGAGTGCGCTAATCTCCAAAAGCCGTCTCAGTTCGGATTGTTCTCTGCAACTCGAGAGCATGAAGGCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCAGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGGTTCACCCGAAGGCGTTGCGCTAACTCGCAAGAGAGGCAGGCGACCACGGTGCTAGCGTGT。
The gene complete sequence of bacterial strain 16S rDNA is analyzed in ncbi database relatively, be accredited as Sphingol single-cell, qualification result is seen Fig. 2, its systematic evolution tree is seen Fig. 3, with Sphingomonas aquatilis strain JSS-7 (AF131295) homology up to 99%, called after Sphingomonas sp.Strain DX-T3-03.
Described Sphingol single-cell Sphingomonas sp.Strain DX-T3-03 is the Gram-negative tyrothricin, and is aerobic, and the thalline size is about (0.4～0.6) μ m * (1.0～2.0) μ m, and Fig. 4 is seen in its Electronic Speculum surface sweeping; Bacterium colony is rounded in solid medium, projection, and milky white is slightly faint yellow, and smooth surface is moistening, and is opaque, and regular edges is neat, very easily provokes; Optimum growing condition pH is about 6.7, and temperature is 30～35 ℃, and shaking table concussion speed is 150r/min.
This Sphingol single-cell (Sphingomonas sp.strain DX-T3-03) can carry out biology absorption and biological accumulation to heavy metal zinc effectively, can be to grow in the 55mM/L zinc concentration solid plate at maximum concentration, and 12h cultivation back just can well growth in the nutrient solution of 25mM/L zinc concentration.
This Sphingol single-cell (Sphingomonas sp.Strain DX-T3-03) can be 0.2,0.4 with concentration, the phenol of 0.6g/L, almost completely degraded in 24h; When phenol concentration 0.8,1.0g/L, can reach about 70% at the 36h degradation rate; The 1.2g/L phenol of will degrading fully also needs certain hour, illustrates that this bacterial strain Pyrogentisinic Acid has degradation capability preferably, and adds 4mM/LZn
2+Can't produce obvious restraining effect to this bacterium degradation of phenol, this is very important to the effect of bacterial strain in the processing that contains the phenol trade effluent that contains heavy metal contamination is used.
Sphingomonas sp.Strain DX-T3-03 has the double effects of degradation of phenol and preventing from heavy metal, because the coexistence of heavy metal and pollutent has suppressed the degradation capability of contaminant degradation bacterium.Therefore, in the extreme environment of heavy metal contamination, only have the bacterium that degradation of organic substances pollutes single ability and will be difficult to bring into play its degradation efficiency, and the screening of this bacterial strain provides reference for the phenol degrading bacterium that utilizes anti-heavy metal carries out biological restoration.
Beneficial effect
Up to 4mM/L the time, the degradation rate of phenol is up to 88% to the tolerance of zinc for bacterial strain of the present invention, and nutritional requirement is not high, and the Pyrogentisinic Acid has degradation capability preferably, contains in phenol trade effluent and the dual pollution of heavy metal at biological treating to have the important application potentiality; Simple and convenient extraction is quick, and cost is low, and is environmentally friendly.
Description of drawings
Fig. 1 is the molecular structural formula of phenol;
Fig. 2 is the qualification result of the 16S rDNA sequence of Sphingomonas sp.Strain DX-T3-03;
Fig. 3 is that tree graph is grown in Sphingomonas sp.Strain DX-T3-03 heredity;
Fig. 4 is the sem photograph of Sphingomonas sp.Strain DX-T3-03;
Bacterium was to different concns phenol degrading rate figure when Fig. 5 was interpolation 4mM/L heavy metal zinc.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
(1) get certain mill tailings dam and fetch earth that to make that soil supension is inoculated in phenol by the access amount of 5wt% be the containing in the zinc minimal medium of sole carbon source, wherein minimal medium is (NH
4)
2SO
410.0g, KH
2PO
47.5g, Na
2HPO
410.0g, FeCl
30.0125g, MgSO
47H
2O 0.015g, distilled water 1000mL, pH=6.5～7.0.The bacteriological filtration phenol and the bacteriological filtration Zn that add gradient concentration simultaneously
2+Solution makes and contains phenol and Zn in the substratum simultaneously
2+And two kinds of material concentrations all raise successively, are phenol 500,600,700,800,900,1000mg/L, Zn
2+Concentration 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0mM/L, be 6～7d the pitch time that changes concentration, in 30～35 ℃, tames under the oscillation rate 150r/min condition and cultivated 2 months;
(2) get the sample of domestication after 2 months, left standstill 30 minutes, get upper strata water sample 1mL, do 10
-1～10
-10Gradient dilution.Every kind of weaker concn is got 1mL bacterium liquid respectively in culture dish, after pouring solid medium into culture dish is put on the table, after coming the back rotation culture dish, culture dish is inverted in 30 ℃ of incubators cultivates 48h, the single clearly bacterium colony of picking form, numbering, on solid medium, rule, culture dish is inverted in 30 ℃ of incubators cultivates 48h, observations again, repeatedly several times, and under electron microscope, observe, guarantee it is behind the pure single bacterial strain, isolated bacterial classification inoculation to slant medium, is stored under 4 ℃ and treats the follow-up test of degrading in the refrigerator.Wherein solid medium is the agar that adds massfraction 1.5% on the enrichment liquid medium base, 1 * 10
5The Pa 30min that sterilizes, cooling back and the substratum that forms, wherein the enrichment liquid nutrient medium is peptone 0.25g, Tryptones 0.25g, yeast powder 0.5g, glucose 0.5g, MgSO
47H
2O 0.03g, distilled water 1000mL, pH=6.5～7.0.Slant medium is got clean test tube for solid medium heating is dissolved, and pours about 5mL solid medium in the test tube into, will try nose end to be placed on the glass stick, makes pipe interior substratum formation inclined-plane and forms;
(3) the difference picking in minimal medium, all contains phenol and the 4mM/L Zn of 0.6g/L through the single bacterium colony after the separation and purification in each minimal medium
2+In 30 ℃, 150r/min, 250mL Erlenmeyer flask, cultivate under the condition of loadings 100mL, incubation time is 24h, cultivate the residual concentration of back, thereby determine phenol efficient degrading bacterial strain with anti-zinc characteristic by phenol in electron microscope observation bacterial growth situation and the mensuration solution.
This bacterial strain is about 6.7 in initial pH value, and temperature is 30 ℃, and oscillation rate is 150r/min, and the liquid amount of 250mL Erlenmeyer flask is under the condition of 100mL, and initial phenol concentration is 0.6g/L, heavy metal Zn
2+Be 4mM/L incubation time 24h, utilize 4-aminoantipyrene method Pyrogentisinic Acid concentration to measure then, the mensuration wavelength is 510nm; The degradation rate of phenol is up to 88%.
Claims (6)
1. Sphingol single-cell DX-T3-03 bacterial strain, it is characterized in that: this bacterial strain has high resistance and has the degradation of phenol ability zinc; Its 16S rDNA sequence is as follows:
CCAATGGCGGCTGCCTACACATGCAGTCGAACGAGATCTTCGGGTCTAGTGGCGCACGG
GTGCGTAACGCGTGGGAATCTGCCCTTTGGTTCGGAATAACAGTTGGAAACGACTGCTA
ATACCGGATGATGACGAAAGTCCAAAGATTTATCGCCAGAGGATGAGCCCGCGTAGGAT
TAGCTAGTTGGTGTGGTAAAGGCGCACCAAGGCGACGATCCTTAGCTGGTCTGAGAGGA
TGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGG
GAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGATGAAGGCCT
TAGGGTTGTAAAGCTCTTTTACCCGGGATGATAATGACAGTACCGGGAGAATAAGCTCCG
GCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC
TGGGCGTAAAGCGCACGTAGGCGGCTTTGTAAGTTAGAGGTGAAAGCCTGGAGCTCAA
CTCCAGAACTGCCTTTAAGACTGCATCGCTTGAATCCAGGAGAGGTGAGTGGAATTCCG
AGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGGCTCACT
GGACTGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCT
GGTAGTCCACGCCGTAAACGATGATAACTAGCTGTCCGGGGACTTGGTCTTTGGGTGGC
GCAGCTAACGCATTAAGTTATCCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAAT
GAATTGACGGGGGCCTGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGC
AGAACCTTACCAGCGTTTGACATGTCCGGACGATTTCCAGAGATGGATCTCTTCCCTTCG
GGGACTGGAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTT
AAGTCCCGCAACGAGCGCAACCCTCGCCTTTAGTTACCATCATTTAGTTGGGGACTCTAA
AGGAACCGCCGGTGATAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCC
TTACGCGCTGGGCTACACACGTGCTACAATGGCGGTGACAGTGGGCAGCAAACTCGCG
AGAGTGCGCTAATCTCCAAAAGCCGTCTCAGTTCGGATTGTTCTCTGCAACTCGAGAGC
ATGAAGGCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCAGG
CCTTGTACACACCGCCCGTCACACCATGGGAGTTGGGTTCACCCGAAGGCGTTGCGCTA
ACTCGCAAGAGAGGCAGGCGACCACGGTGCTAGCGTGT。
2. the extracting method of a Sphingol single-cell DX-T3-03 bacterial strain comprises:
(1) directed domestication
Fetch earth in the mining area and to make soil supension and be inoculated in the minimal medium, with gradient pressure type method for domesticating domestication 2 months by the access amount of 5wt%;
(2) concentration and separation of bacterial strain and purifying
Sample after the above-mentioned domestication is left standstill, get the upper strata water sample and make gradient dilution, every kind of weaker concn is got 1mL bacterium liquid respectively and is carried out enrichment culture in being inoculated into solid medium then; Pure single bacterial strain with cultivating is inoculated into slant medium, gets final product.3. the extracting method of a kind of Sphingol single-cell DX-T3-03 bacterial strain according to claim 2 is characterized in that: the minimal medium in the described step (1) is (NH
4)
25O
410.0g, KH
2PO
47.5g, Na
2HPO
410.0g, FeCl
30.0125g, MgSO
47H
2O 0.015g, distilled water 1000mL, pH=6.5～7.0.
4. the extracting method of a kind of Sphingol single-cell DX-T3-03 bacterial strain according to claim 2 is characterized in that: the gradient pressure type method for domesticating in the described step (1) adds the bacteriological filtration phenol and the bacteriological filtration Zn of gradient concentration simultaneously in minimal medium
2+Solution makes and contains phenol and Zn in the substratum simultaneously
2+And two kinds of material concentrations all raise successively, i.e. phenol 300,400,500,600,700,800,900,1000mg/L, Zn
2+Concentration 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0mM/L, be 6～7d the pitch time that changes concentration, in 30～35 ℃, tames cultivation under the oscillation rate 150r/min condition.
5. the extracting method of a kind of Sphingol single-cell DX-T3-03 bacterial strain according to claim 2 is characterized in that: the gradient dilution in the described step (2) is 10
-1～10
-10Gradient dilution.
6. the extracting method of a kind of Sphingol single-cell DX-T3-03 bacterial strain according to claim 2 is characterized in that: the solid medium in the described step (2) is the agar that adds massfraction 1.5% on the enrichment liquid medium base, 1 * 10
5The Pa 30min that sterilizes, cooling back and the substratum that forms, wherein the enrichment liquid nutrient medium is peptone 0.25g, Tryptones 0.25g, yeast powder 0.5g, glucose 0.5g, MgSO
47H
2O 0.03g, distilled water 1000mL, pH=6.5～7.0.
7. the extracting method of a kind of Sphingol single-cell DX-T3-03 bacterial strain according to claim 2, it is characterized in that: enrichment culture is meant bacterium liquid is cultivated 48h after being inoculated into enrichment medium in 30 ℃ of incubator in the described step (2), the single clearly bacterium colony of picking form, numbering is rule on solid medium, culture dish is inverted in 30 ℃ of incubators cultivates 48h, observations again, under electron microscope, observe colonial morphology, 2～4 times repeatedly, guarantee to grow pure single bacterial strain.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586132A (en) * | 2011-12-14 | 2012-07-18 | 建设部水处理新技术产业化基地(天津港保税区水处理新技术产业化基地) | Sphingobacteria sp. for removing ammonia nitrogen from sewage at low temperature and separate culturing method thereof |
| CN104974968A (en) * | 2015-07-28 | 2015-10-14 | 吉首大学 | Strain YAB-3 for degrading PFOA (perfluorooctanoic acid) and application thereof |
| CN105907688A (en) * | 2016-06-27 | 2016-08-31 | 南京工业大学 | Strain for degrading phenol compounds and application thereof |
| CN108529760A (en) * | 2018-04-26 | 2018-09-14 | 东北农业大学 | The repairing method of microorganism that degradable organic wastewater is coupled with heavy metal polluted waste water |
| CN110257280A (en) * | 2019-05-31 | 2019-09-20 | 华南理工大学 | The Sphingol single-cell and its acclimation method of a kind of triphenyl phosphate that can degrade and application |
| CN112410262A (en) * | 2020-12-07 | 2021-02-26 | 北京工商大学 | New strain of sphingosine monad of North industry and commerce and application thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586132A (en) * | 2011-12-14 | 2012-07-18 | 建设部水处理新技术产业化基地(天津港保税区水处理新技术产业化基地) | Sphingobacteria sp. for removing ammonia nitrogen from sewage at low temperature and separate culturing method thereof |
| CN104974968A (en) * | 2015-07-28 | 2015-10-14 | 吉首大学 | Strain YAB-3 for degrading PFOA (perfluorooctanoic acid) and application thereof |
| CN104974968B (en) * | 2015-07-28 | 2017-10-17 | 吉首大学 | One kind degraded PFOA bacterial strains YAB 3 and its application |
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| CN108529760A (en) * | 2018-04-26 | 2018-09-14 | 东北农业大学 | The repairing method of microorganism that degradable organic wastewater is coupled with heavy metal polluted waste water |
| CN108529760B (en) * | 2018-04-26 | 2022-03-04 | 东北农业大学 | Microbial remediation method for coupling easily-degradable organic wastewater and heavy metal polluted wastewater |
| CN110257280A (en) * | 2019-05-31 | 2019-09-20 | 华南理工大学 | The Sphingol single-cell and its acclimation method of a kind of triphenyl phosphate that can degrade and application |
| CN110257280B (en) * | 2019-05-31 | 2022-04-22 | 华南理工大学 | Sphingosine monad capable of degrading triphenyl phosphate and domestication method and application thereof |
| CN112410262A (en) * | 2020-12-07 | 2021-02-26 | 北京工商大学 | New strain of sphingosine monad of North industry and commerce and application thereof |
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