CN111040965B - Bacterial-algae symbiotic system for strengthening pyridine biodegradation under micro-aerobic condition - Google Patents

Abstract

The invention discloses a symbiotic system of bacteria and algae for strengthening the biodegradation of pyridine under micro-aerobic conditions. The bacteria-algae symbiotic system is formed by the mixed culture of Paracoccus sp. NJUST47 with pyridine degradation function and Chorella sorokiniana FACHB-275 as inoculum. In the symbiotic system of bacteria and algae of the invention, Paracoccus grows with pyridine as the sole carbon source and nitrogen source, and Chlorella grows with ammonia nitrogen produced by pyridine degradation as the nitrogen source. In the symbiotic system of bacteria and algae, microalgae generate dissolved oxygen through photosynthesis, and Paracoccus uses the dissolved oxygen generated by microalgae photosynthesis to achieve efficient degradation of pyridine under microaerobic conditions, and the maximum degradation rate can reach 45.16±2.4mg/L/day , pyridine at a concentration of 100 mg/L can be completely degraded within 60 hours. At the same time, in the symbiotic system of bacteria and algae, the population growth and sedimentation performance of microalgae can be significantly improved.

Description

A bacterial-algal symbiotic system for enhanced pyridine biodegradation under microaerobic conditions

technical field

The invention belongs to the technical field of biological treatment of environmental organic pollutants, and relates to a symbiotic system of bacteria and algae for strengthening the biodegradation of pyridine under micro-aerobic conditions.

Background technique

Pyridine has biotoxic, teratogenic and carcinogenic properties, and its pollution has caused huge potential harm to human health and ecological environment. Biological removal of pyridine-containing wastewater has the advantages of no secondary pollution and good economy, and is currently the most widely used wastewater treatment technology. However, due to the stable structure and poor biodegradability of pyridine, ordinary biological treatment is ineffective or inefficient. In recent years, for refractory pollutants such as pyridine, the method of adding pyridine-degrading functional bacteria for bioaugmentation has gradually attracted the attention of environmental protection workers. Due to the high stability and strong biological toxicity of pyridine, the degradation efficiency of pyridine under anaerobic conditions is low. At present, most of the pyridine-degrading bacteria belong to the genus of aerobic bacteria, and most of the bioaugmentation of pyridine is limited to aerobic organisms. processing system. However, the supply of oxygen in the current aerobic biological treatment system of wastewater mainly relies on mechanical aeration. Due to the volatile nature of pyridine, the aerobic biodegradation system of pyridine has serious air pollution, which is difficult to meet the requirements of practical engineering applications. Therefore, the traditional oxygen supply mode that relies on mechanical aeration needs to be further improved.

In recent years, the application of microalgae in wastewater treatment has received extensive attention. However, most of the symbiotic systems of bacteria and algae are currently used in wastewater with high nitrogen and phosphorus characteristics and low toxicity (such as municipal wastewater, animal husbandry wastewater, etc.). For example, literature 1 uses a symbiotic system of bacteria and algae to remove ammonia nitrogen in anaerobic digestion of pig wastewater (Wang M, Yang H, Ergas S J, et al. Anovel shortcut nitrogen removal process using an algal-bacterial consortium in a photo-sequencing batch reactor (PSBR )[J].Water research, 2015, 87:38-48.), Literature 2 uses a symbiotic system of bacteria and algae to remove COD, TOC, TN and TP ( Van Den Hende S, Carré E, Cocaud E, et al. Treatment of industrialwastewaters by microalgal bacterial flocs in sequencing batch reactors [J]. Bioresource technology, 2014, 161: 245-254.). Reference 3 uses a symbiotic system of bacteria and algae to remove total dissolved nitrogen, total dissolved phosphorus and COD in non-toxic synthetic wastewater (Ji X, Jiang M, Zhang J, et al. The interactions of algae-bacteria symbiotic system and its effects on nutrients removal from synthetic wastewater[J].Bioresource technology, 2018, 247:44-50.). The application of the above bacterial-algal symbiosis system in highly toxic chemical wastewater is still limited. If pyridine-degrading bacteria and microalgae can be used to develop a symbiotic system with pyridine-degrading function, it is expected to achieve high-efficiency degradation of pyridine without external oxygen supply. very important meaning. However, whether microalgae can tolerate the high toxicity of pyridine in the pyridine wastewater treatment system has not yet been reported in the literature.

SUMMARY OF THE INVENTION

Aiming at the pollution problem of pyridine volatilization caused by aeration and oxygen supply in the aerobic biological treatment system of pyridine, the present invention provides a symbiotic system of bacteria and algae for enhancing the biodegradation of pyridine under micro-aerobic conditions. The closed bacteria-algae symbiotic system produces oxygen through microalgae photosynthesis, realizes the enhancement of pyridine biodegradation under micro-aerobic conditions, and provides a culture method for the bacteria-algae symbiotic system with pyridine degradation function.

The present invention first provides a Paracoccus with pyridine degradation function, which is Paracoccus sp.NJUST47, which was deposited in the China Collection of Type Cultures (CCTCC) on May 24, 2019, and the address of the preservation unit is Wuhan University, Wuhan City, Hubei Province, China , the deposit number is CCTCC NO: M2019392.

The inventors used activated sludge for treating pyridine wastewater as the bacterial source, and used the screening medium with pyridine as the sole carbon source and nitrogen source to enrich, purify and separate the strains, and obtained a strain that can use pyridine as the sole source of bacteria. The pyridine-degrading functional strain of carbon and nitrogen sources was identified as Paracoccus by molecular biology and named as Paracoccus sp.NJUST47. Paracoccus sp. NJUST47 could grow using pyridine as the sole carbon and nitrogen source, but the degradation rate of pyridine was low under anaerobic conditions.

The invention also provides the application of the above Paracoccus sp. NJUST47 in the treatment of pyridine-containing wastewater. The specific method is as follows: inoculating the Paracoccus sp. NJUST47 seed liquid into the pyridine-containing wastewater for cultivation, the cultivation temperature is 30°C, and the cultivation pH is 7.2±0.05.

The bacteria-algae symbiotic system for enhancing pyridine biodegradation under micro-aerobic conditions of the present invention is composed of a single species of Paracoccus sp. NJUST47 and a single species of Chlorella sorokiniana FACHB-275 mixed culture.

The Chlorella sorokiniana FACHB-275 of the present invention comes from the Freshwater Algae Seed Bank (FACHB) of the Chinese Academy of Sciences, and has been expanded in the laboratory. The dissolved oxygen concentration can reach a level of more than 5mg/L.

Preferably, when the OD ₆₀₀ of Paracoccus sp. NJUST47 = 0.2: the total pigment of Chorella sorokiniana FACHB-275 is 0.34 mg/L, the ratio of bacteria and algae is 1:1, in the symbiotic system of bacteria and algae, the bacteria and algae are The ratio is 1 to 3:3, more preferably 1 to 2:3.

The present invention provides the application of the above-mentioned symbiotic system of bacteria and algae in the treatment of pyridine-containing wastewater.

Further, the application of the above-mentioned bacteria-algae symbiotic system in the treatment of pyridine-containing wastewater, the concrete method is:

(1) after the Chlorella sorokiniana FACHB-275 is expanded and cultivated, the liquid algae is centrifuged, the residual nutrients are removed by cleaning, and the algal body sediment obtained by centrifugation again is used as the microalgal inoculum;

(2) After Paracoccus sp.NJUST47 is expanded and cultivated, the bacterial liquid is centrifuged, and the residual nutrients are removed by cleaning, and the bacterial sediment obtained by centrifugation is used as the bacterial inoculum;

(3) Mix the microalgae inoculum and the bacterial inoculum in proportion to form the bacterial algae inoculum, add it to the wastewater containing pyridine, use light-dark cycle light, set the temperature to 26-30°C, pH=7.2±0.05, and carry out Degradation of pyridine.

In a specific embodiment of the present invention, in step (1), the medium of Chorella sorokiniana FACHB-275 adopts BG-11 medium.

In a specific embodiment of the present invention, in step (1), the centrifugal speed is 8000 rpm, the centrifugal time is 10 min, and the cleaning solution is a phosphate buffered solution.

In a specific embodiment of the present invention, in step (2), the centrifugal speed is 6000 rpm, the centrifugal time is 10 min, and the cleaning solution is a phosphate buffered solution.

In the specific embodiment of the present invention, in step (3), the light intensity used is 10050 lux.

In the specific embodiment of the present invention, in step (3), the adopted illumination period is light/dark=12h/12h.

The bacteria-algae symbiosis system of the invention can achieve enhanced pyridine biodegradation under micro-aerobic conditions by generating oxygen through microalgae photosynthesis without mechanical aeration and oxygen supply. The pyridine-degrading strain Paracoccus sp. NJUST47 and Chorella sorokiniana FACHB-275 were used as inoculum to form a symbiotic system of bacteria and algae through mixed culture. Further, the degradation of pyridine by the pyridine-degrading bacteria was enhanced under micro-aerobic conditions.

Compared with the prior art, the present invention has the following significant advantages:

(1) The present invention finds for the first time that Chorella sorokiniana FACHB-275 can tolerate the high toxicity of pyridine, and has a synergistic effect with Paracoccus sp. The generation of dissolved oxygen by algae through photosynthesis can significantly promote the biodegradation of pyridine under microaerobic conditions, while removing nitrogen in the system, achieving effective treatment of sewage, and reducing _CO2 emissions, with a maximum pyridine degradation rate of 45.16±2.4mg /L/day, 100mg/L pyridine can be completely degraded within 60 hours;

(2) The bacteria-algae symbiosis system of the present invention can improve the sedimentation performance of the microalgae and improve the quality of the effluent.

Description of drawings

Figure 1 shows the effect of Paracoccus sp. NJUST47 on aerobic degradation of pyridine when using traditional oxygen supply mode, (a) is the change of pyridine concentration, (b) is the change of TOC concentration, (c) is ammonia nitrogen Concentration change graph.

Fig. 2 is a graph showing the changes of pyridine concentration (a) and TOC concentration (b) in pure bacteria Paracoccus sp. NJUST47 system, pure bacteria Chorella sorokiniana FACHB-275 system and bacteria and algae symbiotic system in Example 3.

FIG. 3 is a graph showing the change of dissolved oxygen concentration in the symbiotic system of bacteria and algae in Example 3. FIG.

FIG. 4 is a graph (a) (b) and a graph (c) (d) of the change of pyridine concentration in the symbiotic system of bacteria and algae with different proportions of bacteria and algae in Example 4.

5 is a scanning electron microscope image of microorganisms in pure bacteria Paracoccus sp. NJUST47 system (a), pure Chlorella sorokiniana FACHB-275 system (b) and bacteria and algae symbiosis system (c) in Example 3.

Detailed ways

The present invention will be further described in detail below with reference to specific embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto. For process parameters not particularly noted, reference may be made to conventional techniques.

Example 1

Screening, isolation and identification of Paracoccus sp.NJUST47

(1) Isolation of strains

The existing SBR activated sludge reactor used to treat pyridine wastewater in the laboratory has achieved good results after more than one year of operation. Take the aerobic granular sludge from the SBR reactor, and dilute it to ^10-10 times with sterile water after grinding. Prepare inorganic salt agar solid medium, spread 20 μL of the diluted culture solution on LB agar solid medium containing 500 mg/L pyridine, and culture in a biochemical incubator at 30±2°C for three days. A single colony with obvious differences on the petri dish was selected, purified and cultured by streaking on a plate, and after five consecutive purifications, a single bacterial strain was obtained and stored on a slant. Prepare the inorganic salt liquid medium containing pyridine and put it into a conical flask, inoculate the pure strain obtained by separation and purification after high temperature sterilization at 121 °C, and cultivate in a constant temperature shaking incubator at 180 rpm and 35 °C, and monitor the cultivation process. Changes in the concentration of pyridine. The strain that can effectively remove pyridine in the medium was selected and named as NJUST47, which was stored at -80°C with 20% glycerol.

The composition of LB medium was as follows: tryptone (10 g/L), yeast extract (5 g/L), sodium chloride (10 g/L).

The composition of the inorganic salt medium (MSM) was as follows: Na ₂ HPO ₄ ·12H ₂ O (3.057 g/L), KH ₂ PO ₄ (0.760 g/L), MgCl ₂ ·4H ₂ O (0.136 g/L), CaCl ₂ (0.05g/L), trace element solution SL-4 (10mL/L). Composition of trace element SL-4: EDTA (0.5g/L), FeSO ₄ ·7H ₂ O (0.2g/L), trace element SL-6 (100mL/L). Composition of trace elements SL-6: ZnSO ₄ ·7H ₂ O (0.01g/L), MnCl ₂ ·4H ₂ O (0.03g/L), H ₃ BO ₄ (0.3g/L), CoCl ₂ ·6H ₂ O (0.2 _g /L), _CuCl2.2H2O (0.01 _g /L), NiCl2.6H2O (0.02 _g /L), _{Na2MoO4.2H2O (} 0.03 _g /L). The amount of pyridine was added according to the experimental needs.

Add 2g/L of agar powder to the liquid medium, sterilize by autoclaving at 121°C for 20 minutes in a sterilizing pot, pour it into a sterile petri dish and cool to room temperature to obtain inorganic salt agar solid medium.

(2) Identification of strains

Morphological, physiological and biochemical tests were performed on the strain NJUST47. Determine the 16S rRNA gene sequence of the strain (see SEQ ID NO. 1 in the sequence listing), compare the 16S rRNA gene sequence of the strain with the gene sequence in the GenBank database for homology and analyze the results, and determine the strain from the molecular biology level. 's species.

(2.1) Morphological characteristics: When grown on LB medium, NJUST47 colonies were creamy yellow, with smooth and moist surfaces and neat edges. The cells of this strain were short rod-shaped, with a size of 0.42-0.49 μm×0.85-1.61 μm. Figure 1 and Figure 2 are the solid medium plate colony image and scanning electron microscope image of bacteria NJUST47, respectively.

(2.2) Physiological and biochemical characteristics: Gram-negative, contact enzyme, oxidase-positive, aerobic, inactive, the optimum degradation pH range is 6.5-7.5, and the optimum growth temperature is 30-35°C.

(2.3) Molecular biological identification: Using the nuclear DNA of the NJUST47 strain as a template, PCR amplification was carried out with the universal primers for bacterial amplification, and the gene sequence of the strain NJUST47 was determined. The 16S rRNA gene sequence of the strain was submitted to the GenBank database for homology comparison. The results showed that the sequence similarity between NJUST47 and Paracoccus sp.JQ8-2 was over 98%.

According to the morphological, physiological and biochemical tests and molecular biological analysis of NJUST47, NJUST47 was identified as Paracoccus sp. and named as Paracoccus sp. NJUST47. Paracoccus sp.NJUST47 was deposited in CCTCC, China Type Culture Collection on May 24, 2019. The address of the deposit is Wuhan University, Wuhan City, Hubei Province, China, and the deposit number is CCTCC NO: M 2019392.

Example 2

Effect of Paracoccus sp. NJUST47 on the aerobic biodegradation of pyridine when using conventional oxygen supply mode.

The strain Paracoccus sp. NJUST47 was inoculated into the LB medium containing 100 mg/L pyridine, and cultivated in a shaker at 180 rpm at 30°C to enrich the NJUST47 strain. After the strain entered the logarithmic growth phase (about 48 hours) ), the obtained bacterial liquid is centrifuged for 10 minutes (6000 rev/min) with a centrifuge to obtain sedimentary thalline, resuspended with the sterilized inorganic salt liquid medium, centrifuged, and repeated washing three times, the thalline is resuspended in sterile In liquid inorganic salt medium, seed liquid (control OD ₆₀₀ is about 1.7) is obtained.

The inorganic salt liquid medium with 1500mg/L pyridine as the sole carbon source and nitrogen source was prepared as simulated wastewater, the above-mentioned seed solution was added to the simulated pyridine wastewater, the inoculum amount was 5%, and the inoculum was 180 rpm at 30 °C. The rotating speed shaker was cultured, and the Erlenmeyer flask was sealed with sterile gauze and kept ventilated to maintain aerobic conditions. The concentration changes of pyridine, total organic carbon (TOC) and ammonia nitrogen were monitored during the culture.

Figure 1 shows the effect of Paracoccus sp. NJUST47 on aerobic degradation of pyridine when using traditional oxygen supply mode, (a) is the change of pyridine concentration, (b) is the change of TOC concentration, (c) is Changes in ammonia nitrogen concentration. It can be seen from Figure 1 that Paracoccus sp.NJUST47 can completely degrade pyridine within 37 hours, indicating that the isolated strain Paracoccus sp.NJUST47 can use pyridine as the only carbon and nitrogen source for metabolism and metabolism under aerobic conditions. growth while achieving efficient removal of pyridine.

Example 3

This example is used to illustrate the cultivation of the symbiotic system of bacteria and algae and the process of enhancing the biodegradation of pyridine.

(1) Preparation of inoculum: After culturing Chorella sorokiniana FACHB-275 in BG-11 medium for one month, the liquid algae were centrifuged at 8000 rpm for 10 minutes, and then phosphate buffered solution was used. The residual nutrients were washed away, and the resulting algal body sediment was used as a microalgal inoculum after centrifugation again. Paracoccus sp.NJUST47 was inoculated into a nutrient broth (NB) containing 100mg/L of pyridine with a concentration of 18g/L, and after 48 hours of shaking, the bacterial liquid was centrifuged at 6000 r/min. After 10 minutes, the residual nutrients were washed away with phosphate buffer solution, and the obtained bacterial cell sediment was used as bacterial inoculum after centrifugation again.

(2) Cultivation of the symbiotic system of bacteria and algae: The simulated wastewater of BG-11 with a pyridine concentration of 100 mg/L and no nitrogen source was added to the conical flask. The bacteria and algae inoculum was added to the reaction flask and put into the light incubator for the experiment. The light source was set on the top, and the LED cold light source with the light intensity of 10050 lux was used. The light cycle of light/dark=12h/12h was used, and the temperature was set to 26 ~30°C, pH=7.2±0.05, stirring speed is 100 rpm, the reaction flask is made of glass fully transparent conical flask, equipped with a water quality detector that can detect dissolved oxygen concentration, temperature and pH value. During the reaction, the reaction flask was in a nitrogen operating environment, and the pyridine concentration, total organic carbon concentration, dissolved oxygen concentration, temperature and pH in the system were detected during the reaction. In the treatment of pyridine wastewater, slight differences in light, temperature and stirring conditions will affect the degradation effect of pyridine to a certain extent. Since the suitable temperature of microalgae does not exceed 30 degrees, the temperature is controlled at 26-30 degrees Celsius.

The components of BG-11 medium are: NaNO ₃ 1500mg/L, KH ₂ PO ₄ 3H ₂ O 40mg/L, MgCl ₂ 7H ₂ O 0.16g/L, CaCl ₂ 2H ₂ O 36mg/L, EDTA 1mg/L, Na ₂ CO ₃ 20mg/L, ferric citrate 6mg/L, ferric ammonium citrate 6mg/L, trace element A5+Co 1mL/L (A5+Co mother liquor is H ₃ BO ₃ 2.86g/L; MnCl ₂ ·4H ₂ O 1.81g/L; ZnSO ₄ ·7H ₂ O 0.222g/L; CuSO ₄ ·5H ₂ O 0.079g/L; NaMoO ₄ ·2H ₂ O 0.390g/L; Co(NO ₃ ) ₂ 6H ₂ O 0.0494 g/L).

The composition of the simulated wastewater is: pyridine 100mg/L, Na ₂ HPO ₄ 12H ₂ O 1.53g/L, KH ₂ PO ₄ 0.38g/L, KH ₂ PO ₄ 3H ₂ O 40mg/L, MgCl ₂ 7H ₂ O 0.16g/L, CaCl ₂ ·2H ₂ O 36mg/L, EDTA 1mg/L, Na ₂ CO ₃ 20mg/L, ferric citrate 6mg/L, ferric ammonium citrate 6mg/L, trace elements A5+Co 1mL /L (A5+Co mother liquor is H ₃ BO ₃ 2.86g/L; MnCl ₂ 4H ₂ O 1.81g/L; ZnSO ₄ 7H ₂ O 0.222g/L; CuSO 5H ₂ O 0.079g/L; NaMoO ₄ ·2H ₂ O 0.390 g/L; Co(NO ₃ ) ₂ ·6H ₂ O 0.0494 g/L).

Figure 2 is a graph showing the changes of pyridine concentration (a) and TOC concentration (b) in pure bacteria Paracoccus sp. Fig. 3 is a graph showing the change of dissolved oxygen concentration in the symbiotic system of bacteria and algae. It can be seen from Figure 2 that the pure algae system has no degradation effect on pyridine, and Chlorella cannot degrade pyridine alone. The degradation rate of pyridine in the pure bacteria system was 17.8±0.02 mg/L/day without oxygen supply, and the pyridine degradation rate in the bacteria-algae symbiotic system was 30.82±1.62 mg/L/day. In the symbiotic system of bacteria and algae, the concentration of dissolved oxygen increases with the illumination, and bacteria and microalgae use each other to synergize, resulting in green bacteria and algae co-flocs. The symbionts of bacteria and algae can generate dissolved oxygen through photosynthesis without external aeration, forming a micro-aerobic environment, which can significantly promote the biodegradation of pyridine. After 100mg/L pyridine was completely degraded, the mature symbiotic system of bacteria and algae was basically formed. It can be seen from the scanning electron microscope (SEM) image in Fig. 5 that the short rod-shaped Paracoccus sp. NJUST47 (a) and the spherical Chorella sorokiniana FACHB-275 (b) were mixed and cultured in the simulated wastewater containing pyridine. , forming a bacterial-microalgae symbiosis (c).

Example 4

In this example, on the basis of Example 2, bacteria and microalgae were inoculated in different proportions, and the effects of different inoculation proportions of bacteria and algae on the enhanced pyridine degradation of the symbiotic system of bacteria and algae were discussed.

Figure 4 shows the changes of pyridine concentration (a) (b) and the TOC concentration (c) (d) in the bacteria-algae symbiotic system with different proportions of bacteria and algae. When the ratio of bacteria and algae described in Figure 4 is 1:1, the OD ₆₀₀ of Paracoccus sp. NJUST47 = 0.2: the total pigment of Chorella sorokiniana FACHB-275 is 0.34 mg/L, and the bacteria are changed based on this Inoculation ratio with microalgae.

It can be seen from Figure 4 that increasing the inoculum of microalgae did not significantly improve the degradation effect of pyridine by the symbiotic system of bacteria and algae, while increasing the inoculum of pyridine degrading bacteria can improve the degradation effect of pyridine by the symbiotic system of bacteria and algae to a certain extent , which indicates that in the symbiotic system of bacteria and algae, the role of microalgae is mainly to generate dissolved oxygen through photosynthesis, which promotes the aerobic degradation of pyridine by pyridine-degrading bacteria, and the main role of pyridine-degrading bacteria is to degrade pyridine. Due to the complex coexistence relationship in the symbiotic system of bacteria and algae, the mutual beneficial symbiosis and the competition relationship exist at the same time, so bacteria and microalgae need to maintain a certain proportion to produce good synergy. In the formed symbiotic system of bacteria and algae, all showed better pyridine degradation effect than pure bacteria system. In comparison, the proportion of bacteria and algae inoculated was Paracoccus sp. NJUST47, OD ₆₀₀ = 0.4: Chorella sorokiniana FACHB-275 When the total amount of pigment was 1.07 mg/L, the bacterial-algal symbiotic system showed the best pyridine degradation effect.

sequence listing

<110> Nanjing University of Science and Technology

<120> Symbiotic system of bacteria and algae for enhanced biodegradation of pyridine under micro-aerobic conditions

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<170> SIPOSequenceListing 1.0

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Claims (10)

1. Paracoccus with pyridine degradation function, which is Paracoccus sp. NJUST47, and the deposit number is CCTCCNO: M2019392. 2. the application of Paracoccus according to claim 1 in pyridine-containing waste water treatment, it is characterized in that, concrete method is: Paracoccus Paracoccus sp.NJUST47 seed liquid is inoculated in pyridine-containing waste water, and cultivation temperature is 30 ℃, The culture pH was 7.2±0.05. 3. The bacteria-algae symbiotic system for enhancing the biodegradation of pyridine under micro-aerobic conditions, characterized in that it is composed of a single species of Paracoccus sp. NJUST47 and a single species of Chlorella sorokiniana FACHB-275 mixed culture. 4. The symbiotic system of bacteria and algae according to claim 3, is characterized in that, the ratio of bacteria and algae when the total pigment amount of Chorella sorokiniana FACHB-275 is 0.34mg/L with OD ₆₀₀ of Paracoccus sp.NJUST47=0.2 is 1:1, and in the symbiotic system of bacteria and algae, the ratio of bacteria and algae is 1-3:3. 5 . The symbiotic system of bacteria and algae according to claim 4 , wherein the ratio of bacteria and algae is 1-2:3. 6 . 6. The application of the bacteria-algae symbiotic system according to claim 3 in the treatment of pyridine-containing wastewater. 7. application according to claim 6, is characterized in that, concrete method is: (1) after Chlorella sorokiniana FACHB-275 is expanded and cultivated, the liquid algae is centrifuged, the residual nutrients are removed by cleaning, and the algal body sediment obtained by centrifugation again is used as the microalgal inoculum; (2) after the expansion of Paracoccus sp.NJUST47, the bacterial liquid is centrifuged, and the residual nutrients are removed by cleaning, and the thalline sediment obtained by centrifugation again is used as bacterial inoculum; (3) Mix the microalgae inoculum and the bacterial inoculum in proportion to form the bacterial algae inoculum, add it to the pyridine-containing wastewater, use light-dark cycle light, set the temperature to 26-30°C, pH=7.2±0.05, and carry out Degradation of pyridine. 8. application according to claim 7, is characterized in that, in step (1), the substratum of Chlorella sorokinianaFACHB-275 adopts BG-11 substratum, and described centrifugal speed is 8000 rev/min, centrifugal The time was 10 min, and the washing solution was phosphate buffered solution. 9. application according to claim 7, is characterized in that, in step (2), described centrifugal speed is 6000 rev/min, centrifugal time is 10min, and cleaning solution is phosphate buffered solution. 10. The application according to claim 7, characterized in that, in step (3), the adopted light intensity is 10050 lux, and the adopted light cycle is light/dark=12h/12h.

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