CN106630491A - Method for removing wastewater pollutant containing cadmium - Google Patents
Method for removing wastewater pollutant containing cadmium Download PDFInfo
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- CN106630491A CN106630491A CN201710061962.1A CN201710061962A CN106630491A CN 106630491 A CN106630491 A CN 106630491A CN 201710061962 A CN201710061962 A CN 201710061962A CN 106630491 A CN106630491 A CN 106630491A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/10—Solids, e.g. total solids [TS], total suspended solids [TSS] or volatile solids [VS]
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- C—CHEMISTRY; METALLURGY
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/24—Separation of coarse particles, e.g. by using sieves or screens
Abstract
The invention relates to a method for removing wastewater pollutant containing cadmium. The method comprises the following steps: firstly performing solid-liquid separation on wastewater through a solid-liquid separator, removing bulk solid granular materials, then enabling liquid to enter into a sedimentation tank, so as to precipitate the liquid for 12 hours, enabling the liquid to pass through a round hole filter screen, so as to remove solid flocculate, enabling the liquid filtered through the round hole filter screen to pass through a biological reaction tank, adjusting PH value to be 7, adding 10 grams of multiple species inoculants for every cubic meter of the liquid each time, adding one time every day, continuously adding for one week, finally leaving the liquid to stand for 3 days, and discharging the liquid. The method can effectively remove pollutant containing ammonia, nitrogen, sulphur, phosphorus and cadmium, is low in investment cost, and has a good application prospect.
Description
Technical field
The invention belongs to environmental technology field, and in particular to a kind of method of removal dirt water pollutant.
Background technology
Along with industrial development and the improvement of people's living standards, trade effluent amount and city domestic sewage
Amount just surges with surprising rapidity, these sewage or or has polluted river for the survival of mankind, river and lake, structure
One of the reason for environment for human survival being assisted into prestige.
In order to meet continuous improvement of the public to environmental quality requirement, country has worked out increasingly stricter discharge mark to nitrogen
Standard, research and development are economical, efficiently denitrogenate treatment technology has become the emphasis and focus of Water Pollution Control Engineering area research.Though
So there are many methods effectively to remove ammonia, such as physical method has counter-infiltration, distillation, soil irrigation;Chemical method has ion to hand over
Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysis;Biological method has nitrification and algae
Class is cultivated, but physical method treatment effect is not good, and than chemical method, biological method processes waste water and has the following advantages:1) it is every kind of
The very strong product of being both for property of chemical article, failure is possible to when other chemical substances are run into, and biologic product is to dirt
The removal of dye thing has light spectrality;2) chemical products can temporarily eliminate some harmful substances and cover stink, and lacking to hinder
The only generation of harmful substance;3) after using chemical products, residual is had in water body, secondary pollution may be caused.Biologic product institute
Containing natural microbial, without pathogenic bacteria and pathogen, these microorganisms under the catalytic action of enzyme, with the organic nutrition in sewage
Material is food, and after sewage is purified, these microbes are gradually decreased with the reduction of pollutant, until withering away;4)
Nontoxic, non-corrosiveness is easy to use, and equipment or engineering need not be added substantially, saves fund input.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients is complicated, particularly substantial amounts of artificial conjunction
Environment is entered into compound, this kind of material is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, due to these materials knot itself
The complexity of structure, can not be decomposed by the microorganisms at short notice utilization, and traditional method of wastewater treatment is tamed and dociled with activated sludge culture
The microorganism of change effectively can not be removed to these pollutants, and these materials are accumulated in the environment for a long time, be relied to us
Very big pollution is caused with the ecological environment survived, the physical and mental health for giving people class brings very big harm.Quite a few industry of China
Contaminating enterprises would rather be punished also be unwilling to invest and administer waste water, even if there is sewage-treatment plant operation also abnormal.Therefore, open
Sending out a kind of, construction investment is few, operating cost is low, good treatment efficiency sewage disposal technology is extremely urgent.
The content of the invention
In order to overcome the deficiencies in the prior art, effectively simply remove ammonia nitrogen sulphur phosphorus and heavy metal in sewage etc. and pollute
Thing, the invention provides a kind of method for removing dirty water pollutant, its technical scheme is realized by the following method:
A kind of to remove the method containing cadmium wastewater pollutants, it comprises the steps:
The preparation of composite bacteria agent:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, then stands 6
Hour, it is most rearmounted 4 DEG C at low temperature drying, be dried after water content control 6%, obtain final product;The carrier by bamboo charcoal, shitosan and
Diatomite is according to 2:2:1 mass ratio is mixed to get, particle diameter preferably 10 mesh of bamboo charcoal;
Sewage treatment step:First by sewage through solid-liquid separator, separation of solid and liquid is carried out, remove bulk solid particulate matter
Matter, subsequent liquid enters sedimentation basin, precipitates 12 hours, then liquid is removed into solid floccules, circular hole mistake by circular hole screen pack
The Circularhole diameter of filter screen be 0.1mm, biological oxidation:The liquid of Jing circular hole screen packs enters biological reaction pool, and it is 7 to adjust pH, is pressed
Every cubic metre of liquid adds every time 10 grams of composite bacteria agent, adds daily 1 time, continuously adds one week, finally 3 days is stood, by liquid
Discharge.
Preferably, the composite bacteria agent is mixed by the raw material bacterium of following weight portion:
10 parts of Rhodococcus sp, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingol single-cell, bacillus pumilus
5 parts, 2 parts of Phanerochaete chrysosporium;The concentration of above-mentioned each raw material bacterium is controlled in (1-2) × 108Individual/ml.Above-mentioned bacterial classification can be with
It is the conventional bacterial strain of prior art;
Preferably,
The Rhodococcus sp is Rhodococcus sp (Rhodococcusrhodochrous) ATCC15906;
The thiobacillus denitrificans are thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259;
The Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107;
The Sphingol single-cell is Sphingol single-cell (Sphingomonas sp.) CGMCC N0.4589;
The bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142;
The Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium)
ATCC24725。
Bacterium of the present invention can be from the business such as CGMCC, CCTCC and American Type Culture collection warehousing (ATCC)
Approach is commercially available.
Preferably, described Phanerochaete chrysosporium is the encoding gene for having proceeded to following any one protein variant of coding
Phanerochaete chrysosporium (Phanerochaete chrysosporium) bacterial strains of ATCC 24725, the protein variant is relative
In albumen original amino acid be mutated as follows respectively 71S/T, 89T/Y, 121T/A, 143D/S, 184S/Q, 239W/G,
246V/P, 269K/N, 304P/S, 353K/E, 369G/D, 372C/S, 422D/R, 448G/I, albumen original amino acid ginseng
See GenBank:NP_744955.1.
Present invention simultaneously provides a kind of transgenic strain for being capable of efficient absorption cadmium, the bacterial strain by by amino acid sequence such as
Genbank:Sequence shown in NP_744955.1 is imported to and realize in Phanerochaete chrysosporium its purpose.
And described amino acid sequence can have a mutation in following different sites, 71S/T, 89T/Y, 121T/A,
143D/S、184S/Q、239W/G、246V/P、269K/N、304P/S、353K/E、369G/D、372C/S、422D/R、448G/I。
(71S/T represents that the S amino acid substitutions in original series the 71st are T amino acid).Amino acid sequence after these mutation is not grudged
The bacterial strain for having imported the amino acid sequence after the mutation equally has similar effect.And applicant is by substantial amounts of experiment card
Real, not all replacing all have similar effect, there is Phanerochaete chrysosporium and other bacterium after substantial amounts of replacement
Strain does not have together the effect of preferable sewage disposal.
Using DNAMAN software Design primers, BamHI and SalI restriction enzyme sites are separately added into, according to amino acid sequence
The described nucleotide sequence HP genes of NP_744955.1 full genomes synthesis, by carrying out amplification purpose fragment, PCR amplifications are obtained
Acquisition genes of interest HP (while corresponding mutational site is incorporated in gene order by multiplex PCR, so as to obtain difference
Mutant gene), double digestion PCR primer is carried out with BamHI and SalI, with the expression for also passing through BamHI and SalI double digestions
Carrier PWB980 is connected, and the recombinant plasmid transformed being proved to be successful is entered in the Phanerochaete chrysosporium, obtains degraded cadmium
Genetic engineering bacterium.
The Amplification Culture of each bacterial classification of the present invention is the cellar culture mode of this area, it is also possible to described in reference literature
Training method obtain.
What the present invention was obtained mainly has the beneficial effect that:The present invention processes sewage using pure microbial inoculum, has effectively purified dirt
Water, and dephosphorization, denitrogenation removal simultaneously, the heavy metal cadmium that can remove sewage Central China simultaneously on this basis solves traditional work
Dephosphorization and denitrification effect are difficult to while take into account the contradiction of heavy metals removal in skill, with preferable application prospect.
Specific embodiment
Embodiment 1
A kind of method for removing dirty water pollutant, it comprises the steps:
The preparation of composite bacteria agent:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, then stands 6
Hour, it is most rearmounted 4 DEG C at low temperature drying, be dried after water content control 6%, obtain final product;Carrier is by bamboo charcoal, shitosan and diatom
Soil is according to 2:2:1 mass ratio is mixed to get;Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight portion:Rhodococcus sp
10 parts, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingol single-cell, 5 parts of bacillus pumilus, yellow archespore hair is put down
2 parts of lead fungi;The concentration of above-mentioned each raw material bacterium is controlled 1 × 108Individual/ml.
Sewage disinfection treatment:First by sewage (NH3-N is 300mg/L, sulfide 80mg/L, is 70mg/L containing phosphor contaminant,
Cadmium content is 40mg/ml, through solid-liquid separator, carries out separation of solid and liquid, removes bulk solid particulate matter, and subsequent liquid is entered
Sedimentation basin, precipitates 12 hours, then liquid is removed into solid floccules by circular hole screen pack, and the Circularhole diameter of circular hole screen pack is
0.1mm;
Biological oxidation:The liquid of Jing circular hole screen packs enters biological reaction pool, and it is 7.0 to adjust pH, by every cubic metre of liquid
10 grams of composite bacteria agent being added every time, being added I time daily, continuously added one week, finally stand 3 days, liquid is discharged.After testing,
The content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, is 2.5mg/L containing phosphor contaminant, and clearance reaches
More than 95%;And cadmium content is 37mg/mL, removal effect is not notable.
Embodiment 2
Using DNAMAN software Design primers, BamHI and SalI restriction enzyme sites are separately added into, according to amino acid sequence
The described nucleotide sequence HP genes of NP_744955.1 full genomes synthesis, by carrying out amplification purpose fragment, PCR amplifications are obtained
Genes of interest HP is obtained (while passing through multiplex PCR by corresponding mutational site 71S/T, 89T/Y, 121T/A, 143D/S, 184S/
Q, 239W/G, 246V/P, 269K/N, 304P/S, 353K/E, 369G/D, 372C/S, 422D/R, 448G/I are incorporated into gene sequence
In row, so as to obtain different mutants gene), double digestion PCR primer is carried out with BamHI and SalI, and also pass through BamHI
It is connected with the cloning vector PWB980 of SalI double digestions, the recombinant plasmid transformed being proved to be successful is entered into into the yellow archespore
In flat lead fungi (Phanerochaete chrysosporium) ATCC24725 of hair, the genetic engineering bacterium of degraded cadmium is obtained.
The Phanerochaete chrysosporium genetic engineering bacterium of embodiment 3 is verified with the wastewater treatment efficiency of remaining several microbial inoculum
According to the method for embodiment 1, corresponding sewage disposal experiment is carried out, its sewage belongs to same with the sewage of embodiment 1
One batch, the pollutant levels with same concentrations.The each group of wherein microbial inoculum is grouped into identical with embodiment 1.
The genetic engineering bacterium that the different mutational site of the acquisition of embodiment 2 is respectively adopted is removed the reaction of cadmium, passes through
Experiment find, 71S/T, 89T/Y, 121T/A, 143D/S, 184S/Q, 239W/G, 246V/P, 269K/N, 304P/S, 353K/E,
369G/D, 372C/S, 422D/R, 448G/I this several mutant bacteria all have and have the effect being remarkably reinforced relative to original bacteria.
It is not more than total processing time 4 days, its result is as follows:Sewage is with a collection of sewage, so as to guaranteed conditions are consistent.
Each pollutant levels of sewage:NH3-N is 300mg/L, sulfide 80mg/L, is 70mg/L containing phosphor contaminant, cadmium
Content is 40mg/ml.
As can be seen from the above results, the Phanerochaete chrysosporium for being built using genetic engineering is constituted with other several bacterial strains
Microbial inoculum in addition to the original preferable effect for removing ammonia nitrogen, sulfide and phosphorus, also with preferably removing cadmium
Effect, and in terms of ammonia nitrogen, sulfide and phosphorus is removed engineered Phanerochaete chrysosporium and other several bacterial strains it
Between also have enhanced synergy.
Claims (4)
1. a kind of to remove the method containing cadmium wastewater pollutants, it comprises the steps:First by sewage through solid-liquid separator, enter
Row separation of solid and liquid, removes bulk solid particulate matter, and subsequent liquid enters sedimentation basin, precipitates 12 hours, then by liquid by circle
Hole screen pack removes solid floccules, and the liquid of Jing circular hole filter screen filtrations enters biological reaction pool, and it is 7 to adjust PH, by often standing
Square rice liquid adds every time 10 grams of composite bacteria agent, adds daily 1 time, continuously adds one week, finally stands 3 days, and liquid is discharged.
2. the method for claim 1, it is characterised in that:The composite bacteria agent is by the raw material bacterium mixing of following weight portion
Into:10 parts of Rhodococcus sp, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingol single-cell, 5 parts of bacillus pumilus,
2 parts of Phanerochaete chrysosporium;The concentration of above-mentioned each raw material bacterium is controlled in (1-2) × 108Individual/ml.
3. method as claimed in claim 2, it is characterised in that:Described Phanerochaete chrysosporium is as follows to have proceeded to coding
Phanerochaete chrysosporium (Phanerochaete chrysosporium) bacterium of ATCC 24725 of the encoding gene of protein variant
Strain, the protein variant is to be mutated as follows relative to albumen original amino acid:369G/D, albumen Original amino
Sequence is referring to GenBank:NP_744955.1.
4. a kind of protein variant, it is characterised in that:The protein variant be carry out relative to albumen original amino acid it is as follows
Mutation:369G/D, albumen original amino acid is referring to GenBank:NP_744955.1.
Priority Applications (1)
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CN201710061962.1A CN106630491B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
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CN201510441587.4A CN105130100B (en) | 2015-07-26 | 2015-07-26 | It is a kind of to remove the method containing cadmium wastewater pollutants |
CN201710061962.1A CN106630491B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
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CN201710061959.XA Pending CN106630490A (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061960.2A Active CN106542706B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201611187983.XA Active CN106746216B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061963.6A Active CN106745817B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061967.4A Pending CN106630493A (en) | 2015-07-26 | 2015-07-26 | Method for removing contaminants from cadmium-containing sewage |
CN201710061966.XA Pending CN106630492A (en) | 2015-07-26 | 2015-07-26 | Method for removing pollutant in cadmium-containing sewage |
CN201710061962.1A Active CN106630491B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201510441587.4A Expired - Fee Related CN105130100B (en) | 2015-07-26 | 2015-07-26 | It is a kind of to remove the method containing cadmium wastewater pollutants |
CN201710061964.0A Active CN106517702B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061971.0A Active CN106630495B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061958.5A Pending CN106517701A (en) | 2015-07-26 | 2015-07-26 | Method for removing pollutants in cadmium-containing sewage |
CN201710061968.9A Expired - Fee Related CN106630494B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061945.8A Active CN106630488B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061957.0A Pending CN106630489A (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
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CN201710061960.2A Active CN106542706B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201611187983.XA Active CN106746216B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061963.6A Active CN106745817B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061967.4A Pending CN106630493A (en) | 2015-07-26 | 2015-07-26 | Method for removing contaminants from cadmium-containing sewage |
CN201710061966.XA Pending CN106630492A (en) | 2015-07-26 | 2015-07-26 | Method for removing pollutant in cadmium-containing sewage |
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CN201510441587.4A Expired - Fee Related CN105130100B (en) | 2015-07-26 | 2015-07-26 | It is a kind of to remove the method containing cadmium wastewater pollutants |
CN201710061964.0A Active CN106517702B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061971.0A Active CN106630495B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061958.5A Pending CN106517701A (en) | 2015-07-26 | 2015-07-26 | Method for removing pollutants in cadmium-containing sewage |
CN201710061968.9A Expired - Fee Related CN106630494B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
CN201710061945.8A Active CN106630488B (en) | 2015-07-26 | 2015-07-26 | Method for removing cadmium-containing sewage pollutants |
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CN111066572B (en) * | 2019-12-27 | 2022-01-14 | 福州康来生物科技有限公司 | Cultivation process of agaricus blazei murill with low heavy metal content |
CN114317373B (en) * | 2022-01-14 | 2023-05-02 | 湘潭大学 | Sphingomonas PAH02, microbial preparation and application thereof as crop cadmium-reducing selenium-enriched functional conditioner |
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CN106746216A (en) | 2017-05-31 |
CN106745817B (en) | 2020-12-15 |
CN106630494B (en) | 2021-10-29 |
CN106630495B (en) | 2021-10-15 |
CN106630493A (en) | 2017-05-10 |
CN106746216B (en) | 2020-04-28 |
CN106517702B (en) | 2021-03-12 |
CN106630490A (en) | 2017-05-10 |
CN105130100B (en) | 2017-06-06 |
CN106630494A (en) | 2017-05-10 |
CN106745817A (en) | 2017-05-31 |
CN106630488A (en) | 2017-05-10 |
CN106517701A (en) | 2017-03-22 |
CN106630488B (en) | 2021-09-24 |
CN106517702A (en) | 2017-03-22 |
CN106630495A (en) | 2017-05-10 |
CN106630491B (en) | 2021-10-15 |
CN106630489A (en) | 2017-05-10 |
CN106542706A (en) | 2017-03-29 |
CN105130100A (en) | 2015-12-09 |
CN106542706B (en) | 2021-07-09 |
CN106630492A (en) | 2017-05-10 |
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