CN106430865B - A method of removing mercurous wastewater pollutants - Google Patents
A method of removing mercurous wastewater pollutants Download PDFInfo
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- CN106430865B CN106430865B CN201611188009.5A CN201611188009A CN106430865B CN 106430865 B CN106430865 B CN 106430865B CN 201611188009 A CN201611188009 A CN 201611188009A CN 106430865 B CN106430865 B CN 106430865B
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 10
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 10
- 239000002351 wastewater Substances 0.000 title claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 241000894006 Bacteria Species 0.000 claims abstract description 25
- 239000010865 sewage Substances 0.000 claims abstract description 25
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
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- 239000007787 solid Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 239000013618 particulate matter Substances 0.000 claims abstract description 4
- 238000004062 sedimentation Methods 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 238000009288 screen filtration Methods 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 241000194103 Bacillus pumilus Species 0.000 claims description 6
- 241001509286 Thiobacillus denitrificans Species 0.000 claims description 6
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 5
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 5
- 230000035772 mutation Effects 0.000 claims description 5
- 241001135759 Sphingomonas sp. Species 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052753 mercury Inorganic materials 0.000 abstract description 5
- CPJBEAMIONWDDK-UHFFFAOYSA-N [N].N.[P].[S] Chemical compound [N].N.[P].[S] CPJBEAMIONWDDK-UHFFFAOYSA-N 0.000 abstract description 2
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 12
- 241000736131 Sphingomonas Species 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 238000011282 treatment Methods 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 6
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000010353 genetic engineering Methods 0.000 description 6
- 241000589614 Pseudomonas stutzeri Species 0.000 description 5
- 125000003275 alpha amino acid group Chemical group 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
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- 241001330002 Bambuseae Species 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- CVTZKFWZDBJAHE-UHFFFAOYSA-N [N].N Chemical class [N].N CVTZKFWZDBJAHE-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
Abstract
The present invention relates to a kind of methods for removing mercurous wastewater pollutants, include the following steps: first by sewage by solid-liquid separator, it is separated by solid-liquid separation, remove bulk solid particulate matter, subsequent liquid enters sedimentation basin, precipitating 12 hours, liquid is removed into solid floccules by circular hole filter screen again, liquid through circular hole filter screen filtration enters biological reaction pool, and adjusting PH is 7, adds 10 grams of composite bacteria agent every time by every cubic metre of liquid, it adds 1 time daily, it continuously adds one week, finally stands 3 days, liquid is discharged.Method of the invention can be effectively removed the pollutant containing ammonia nitrogen sulphur phosphorus and mercury, and input cost is low, have preferable application prospect.
Description
Technical field
The invention belongs to environmental technology fields, and in particular to a method of remove dirty water pollutant.
Background technique
Along with industrial development and the improvement of people's living standards, trade effluent amount and city domestic sewage
Amount just surges at an amazing speed, these sewage or or is polluting river, river and lake for the survival of mankind, structure
One of the reason of assisting environment for human survival at prestige.
In order to meet continuous improvement of the public to environmental quality requirement, country has worked out the discharge mark being increasingly stringenter to nitrogen
Standard, research and development are economical, efficiently denitrogenate emphasis and hot spot that processing technique has become Water Pollution Control Engineering area research.Though
It so can be effectively removed ammonia there are many method, as physical method has reverse osmosis, distillation, soil to irrigate；Chemical method has ion friendship
Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysis；Biological method has nitrification and algae
Class cultivation, however physical method treatment effect is bad, compared with chemical method, biological method processing waste water has the following advantages: 1) every kind
Chemical article is possible to fail both for the very strong product of property when encountering other chemical substances, and biological agent is to dirt
The removal for contaminating object has spectrum；2) chemical products can temporarily eliminate certain harmful substances and cover stink, and lacking cannot hinder
The only generation of harmful substance；3) using after chemical products, residual is had in water body, may cause secondary pollution.Biological agent institute
Containing natural microbial, pathogenic bacteria and pathogen are free of, these microorganisms are under the catalytic action of enzyme, with the organic nutrition in sewage
Substance is food, and after purifying the sewage, these microbes are gradually decreased with the reduction of pollutant, until withering away；4)
It is nontoxic, it is non-corrosive, it is easy to use, addition equipment or engineering are not needed substantially, save capital investment.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients is complicated, especially a large amount of artificial conjunction
Enter environment at compound, this substance is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, since these substances itself are tied
The complexity of structure, cannot be decomposed by the microorganisms utilization in a short time, and traditional wastewater treatment method is tamed and dociled with activated sludge culture
The microorganism of change cannot effectively remove these pollutants, these substances accumulate in the environment for a long time, rely to us
Very big pollution is caused with the ecological environment of existence, brings very big harm to the physical and mental health of the mankind.Quite a few industry of China
Contaminating enterprises, which would rather be punished, is also unwilling that waste water is administered in investment, abnormal having sewage-treatment plant operation.Therefore, it opens
Sending out a kind of, construction investment is few, operating cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Summary of the invention
For overcome the deficiencies in the prior art, the pollution such as ammonia nitrogen sulphur phosphorus and heavy metal in sewage is effectively simply removed
Object, the present invention provides a kind of method for removing dirty water pollutant, technical solution is realized by the following method:
A method of removing mercurous wastewater pollutants comprising following steps:
The preparation of composite bacteria agent: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs evenly, is then allowed to stand 6
Hour, low temperature drying at most 4 DEG C of postposition, it is dry after water content control 6% to get；The carrier by bamboo charcoal, chitosan and
Diatomite is mixed to get according to the mass ratio of 2:2:1, the partial size of bamboo charcoal preferably 10 mesh；
Sewage disinfection treatment: passing through solid-liquid separator for sewage first, be separated by solid-liquid separation, and removes bulk solid particulate matter
Matter, subsequent liquid enter sedimentation basin, precipitate 12 hours, then liquid is removed solid floccules, circular hole mistake by circular hole filter screen
The Circularhole diameter of strainer is 0.1mm, and biological oxidation: the liquid through circular hole filter screen enters biological reaction pool, and adjusting pH is 7, is pressed
Every cubic metre of liquid adds 10 grams of composite bacteria agent every time, adds daily 1 time, continuously adds one week, finally 3 days is stood, by liquid
Discharge.
Preferably, the composite bacteria agent is mixed by the raw material bacterium of following parts by weight:
10 parts of Rhodococcus sp, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, bacillus pumilus
5 parts, 2 parts of Phanerochaete chrysosporium；The concentration of above-mentioned each raw material bacterium is controlled at (1-2) × 108A/ml.Above-mentioned strain can be with
It is the conventional bacterial strain of the prior art,
Preferably,
The Rhodococcus sp is Rhodococcus sp (Rhodococcusrhodochrous) ATCC15906；
The thiobacillus denitrificans are thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259；
The Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107；
The Sphingomonas is Sphingomonas (Sphingomonas sp.) CGMCC N0.4589；
The bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142；
The Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium)
ATCC24725。
Bacterium of the present invention can be from the business such as CGMCC, CCTCC and American Type Culture collection warehousing (ATCC)
Approach is commercially available.
Preferably, the Sphingomonas has been transferred to the sphingol for encoding the encoding gene of any one following protein variant
Sporangium (Sphingomonas sp.) CGMCC N0.4589, the protein variant are relative to PPKL1 albumen Original amino
Sequence carry out being mutated as follows respectively 21L/Q, 59F/S, 75R/V, 121E/D, 165A/P, 199H/R, 242D/V, 421L/Y,
480A/S, 544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V, albumen original amino acid referring to
GenBank:NP_744926.1.
Be capable of the transgenic strain of efficient absorption mercury present invention simultaneously provides one kind, the bacterial strain by by amino acid sequence such as
Sequence shown in Genbank:NP_744926.1, which is imported into Sphingomonas, realizes its purpose.
And the amino acid sequence can have the mutation in following unused site, 21L/Q, 59F/S, 75R/V,
121E/D、165A/P、199H/R、242D/V、421L/Y、480A/S、544R/I、602A/L、690N/Q、729T/G、842A/G、
901Y/V.(21L/Q indicates that in original series the 21st L amino acid substitution be Q amino acid).Amino acid sequence after these mutation
Arranging the bacterial strain for not being willing the amino acid sequence after having imported the mutation equally has similar effect.And applicant passes through largely in fact
Verifying is real, and not all substitution all has similar effect, have Sphingomonas after a large amount of replace with it is other
Bacterial strain together and do not have preferable sewage treatment effect.
Using DNAMAN software Design primers, it has been separately added into BamHI and SalI restriction enzyme site, it is complete according to amino acid sequence
Nucleotide sequence PDA gene described in gene chemical synthesis obtains target fragment by carrying out amplification, and PCR amplification obtains target gene
PDA (while corresponding mutational site being introduced into gene order by multiplex PCR, to obtain different mutants base
Cause), double digestion PCR product is carried out with BamHI and SalI, with the expression vector pBPSE for also passing through BamHI and SalI double digestion
It is connected, the recombinant plasmid transformed being proved to be successful is entered in the Sphingomonas, obtains the genetic engineering bacterium of degradation mercury.
The expansion culture of each strain of the invention is the routine culture mode of this field, is also referred to record in document
Training method obtain.
What the present invention obtained mainly has the beneficial effect that: the present invention handles sewage using pure microbial inoculum, has effectively purified dirt
Water, and dephosphorization, denitrogenation remove simultaneously, and the heavy metal Hg that can remove sewage Central China simultaneously on this basis solves traditional work
Dephosphorization and denitrification effect are difficult to combine the contradiction of heavy metals removal in skill, with good application prospect.
Specific embodiment
Embodiment 1
A method of removing dirty water pollutant comprising following steps:
The preparation of composite bacteria agent: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs evenly, is then allowed to stand 6
Hour, low temperature drying at most 4 DEG C of postposition, it is dry after water content control 6% to get；Carrier is by bamboo charcoal, chitosan and diatom
Soil is mixed to get according to the mass ratio of 2:2:1；Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following parts by weight: Rhodococcus sp
10 parts, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of bacillus pumilus, yellow archespore hair is flat
2 parts of lead fungi；The concentration of above-mentioned each raw material bacterium is controlled 1 × 108A/ml.
Sewage disinfection treatment: being first 300mg/L, sulfide 80mg/L by sewage NH3-N, be 70mg/L containing phosphor contaminant,
Hg content is that 20mg/ml is separated by solid-liquid separation by solid-liquid separator, removes bulk solid particulate matter, and subsequent liquid enters
Sedimentation basin precipitates 12 hours, then liquid is removed solid floccules by circular hole filter screen, and the Circularhole diameter of circular hole filter screen is
0.1mm,
Biological oxidation: the liquid through circular hole filter screen enters biological reaction pool, and adjusting pH is 7.0, by every cubic metre of liquid
10 grams of composite bacteria agent are added every time, is added I times daily, is continuously added one week, is finally stood 3 days, liquid is discharged.Through detecting,
The content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, is 2.5mg/L containing phosphor contaminant, and removal rate reaches
95% or more；And Hg content is 15mg/mL, removal effect is not significant.
Embodiment 2
Using DNAMAN software Design primers, it has been separately added into BamHI and SalI restriction enzyme site, it is complete according to amino acid sequence
Nucleotide sequence PDA gene described in gene chemical synthesis obtains target fragment by carrying out amplification, and PCR amplification obtains target gene
PDA (while by multiplex PCR by corresponding mutational site 21L/Q, 59F/S, 75R/V, 121E/D, 165A/P, 199H/R,
242D/V, 421L/Y, 480A/S, 544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V are introduced into gene order
In, to obtain different mutants gene), carry out double digestion PCR product with BamHI and SalI, with also pass through BamHI and
The cloning vector pBPSE of SalI double digestion is connected, and the recombinant plasmid transformed being proved to be successful is entered the sphingol list
In spore bacterium CGMCC N0.4589, the genetic engineering bacterium of degradation mercury is obtained.
The wastewater treatment efficiency of 3 Sphingomonas genetic engineering bacterium of embodiment and remaining several microbial inoculum is verified
According to the method for embodiment 1, corresponding sewage treatment experiment is carried out, sewage and the sewage of embodiment 1 belong to together
One batch, the pollutant concentration with same concentrations.Wherein each group of microbial inoculum is grouped as identical with embodiment 1.
The genetic engineering bacterium that the different mutational sites that embodiment 2 is prepared are respectively adopted is removed the reaction of mercury,
Be found through experiments that, 21L/Q, 59F/S, 75R/V, 121E/D, 165A/P, 199H/R, 242D/V, 421L/Y, 480A/S,
544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V these types mutant bacteria all have relative to original bacteria with bright
The effect of aobvious enhancing.Total processing time is not more than 4 days, and result is as follows: sewage is with a collection of sewage, thus guaranteed conditions one
It causes.
Each pollutant concentration of sewage: NH3-N 300mg/L, sulfide 80mg/L are 70mg/L, Hg containing phosphor contaminant
Content is 20mg/ml.
As can be seen from the above results, it is constituted using the Sphingomonas and other several bacterial strains of genetic engineering building
Microbial inoculum also has the effect of preferable removal Hg other than having the effect of original preferable removal ammonia nitrogen, sulfide and phosphorus
Fruit, and in terms of removal ammonia nitrogen, sulfide and the phosphorus between the Sphingomonas and other several bacterial strains of genetic engineering also
Synergistic effect with enhancing.
Claims (2)
1. a kind of method for removing mercurous wastewater pollutants, characterized by the following steps: sewage is passed through solid-liquid first
Separator is separated by solid-liquid separation, and bulk solid particulate matter is removed, and subsequent liquid enters sedimentation basin, is precipitated 12 hours, then by liquid
Body removes solid floccules by circular hole filter screen, and the liquid through circular hole filter screen filtration enters biological reaction pool, adjusts pH and is
7,10 grams of composite bacteria agent are added every time by every cubic metre of liquid, is added daily 1 time, are continuously added one week, are finally stood 3 days, it will
Liquid discharge；The composite bacteria agent is mixed by the raw material bacterium of following parts by weight: 10 parts of Rhodococcus sp, 9 parts of thiobacillus denitrificans, being applied
7 parts of family name pseudomonad, 6 parts of Sphingol single-cell, 5 parts of bacillus pumilus, 2 parts of Phanerochaete chrysosporium；Above-mentioned each raw material bacterium
Concentration control at (1-2) × 108A/ml；The Sphingol single-cell has been transferred to the coding base of the following protein variant of coding
Sphingol single-cell (Sphingomonas sp.) CGMCC N0.4589 of cause, the protein variant are relative to PPKL1 albumen
Original amino acid carries out following mutation 59F/S, and albumen original amino acid is referring to GenBank:NP_744926.1.
2. a kind of protein variant, it is characterised in that: the protein variant is as follows relative to the progress of albumen original amino acid
Mutation: 59F/S, albumen original amino acid is referring to GenBank:NP_744926.1.
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| CN201710100910.0A Active CN106830526B (en) | 2015-07-26 | 2015-07-26 | Method for removing mercury-containing sewage pollutants |
| CN201710101106.4A Active CN106865781B (en) | 2015-07-26 | 2015-07-26 | Method for removing mercury-containing sewage pollutants |
| CN201710101117.2A Pending CN106830530A (en) | 2015-07-26 | 2015-07-26 | A kind of method for removing mercurous wastewater pollutants |
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| CN105130101A (en) | 2015-12-09 |
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| CN106746371B (en) | 2021-10-22 |
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| CN106865781B (en) | 2021-08-20 |
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| CN106746369B (en) | 2021-11-05 |
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| CN106430865A (en) | 2017-02-22 |
| CN106745823A (en) | 2017-05-31 |
| CN106830530A (en) | 2017-06-13 |
| CN106830526A (en) | 2017-06-13 |
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