CN106430865A - Method for removing mercury-containing sewage pollutants - Google Patents
Method for removing mercury-containing sewage pollutants Download PDFInfo
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- CN106430865A CN106430865A CN201611188009.5A CN201611188009A CN106430865A CN 106430865 A CN106430865 A CN 106430865A CN 201611188009 A CN201611188009 A CN 201611188009A CN 106430865 A CN106430865 A CN 106430865A
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- 239000010865 sewage Substances 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 10
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 10
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 title abstract description 7
- 229910052753 mercury Inorganic materials 0.000 title abstract 3
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims description 24
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 241000736131 Sphingomonas Species 0.000 claims description 12
- 239000002131 composite material Substances 0.000 claims description 7
- 241000194103 Bacillus pumilus Species 0.000 claims description 6
- 241000589614 Pseudomonas stutzeri Species 0.000 claims description 6
- 241001509286 Thiobacillus denitrificans Species 0.000 claims description 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 6
- 235000001014 amino acid Nutrition 0.000 claims description 6
- 235000018102 proteins Nutrition 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 5
- 241000187694 Rhodococcus fascians Species 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 5
- 239000002351 wastewater Substances 0.000 claims description 5
- 230000035772 mutation Effects 0.000 claims description 4
- 241001135759 Sphingomonas sp. Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000013618 particulate matter Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 238000009288 screen filtration Methods 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 4
- 239000011574 phosphorus Substances 0.000 abstract description 4
- 229910021529 ammonia Inorganic materials 0.000 abstract description 3
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract 1
- 238000007599 discharging Methods 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 229910052717 sulfur Inorganic materials 0.000 abstract 1
- 239000011593 sulfur Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- 238000011282 treatment Methods 0.000 description 7
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000010353 genetic engineering Methods 0.000 description 5
- 230000029087 digestion Effects 0.000 description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
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- 241000195493 Cryptophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- CVTZKFWZDBJAHE-UHFFFAOYSA-N [N].N Chemical class [N].N CVTZKFWZDBJAHE-UHFFFAOYSA-N 0.000 description 1
- CPJBEAMIONWDDK-UHFFFAOYSA-N [N].N.[P].[S] Chemical compound [N].N.[P].[S] CPJBEAMIONWDDK-UHFFFAOYSA-N 0.000 description 1
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- 238000012549 training Methods 0.000 description 1
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- 238000003911 water pollution Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
Abstract
The invention relates to a method for removing mercury-containing sewage pollutants. The method comprises the following steps: firstly enabling sewage to pass through a solid-liquid separator, carrying out solid-liquid separation, removing large solid particulate matters, subsequently introducing liquid into a precipitation tank for precipitating for 12 hours, enabling the liquid to pass through a circular-hole filtering net for removing solid flocculate, introducing the liquid filtered by the circular-hole filtering net into a biological reaction tank, regulating pH to be 7, adding 10g of a compound microbial agent into per cubic meter of the liquid in each time, adding the compound microbial agent once a day, continuously adding the compound microbial agent for one week, and finally standing for 3 days and discharging the liquid. The method is capable of effectively removing pollutants containing ammonia, nitrogen, sulfur, phosphorus and mercury, is low in investment cost and has a good application prospect.
Description
Technical field
The invention belongs to environmental technology field, and in particular to a kind of method of removal dirt water pollutant.
Background technology
Along with industrial development and the improvement of people's living standards, trade effluent amount and city domestic sewage
Amount just surges with surprising rapidity, these sewage or or has polluted river for the survival of mankind, river and lake, structure
One of the reason for becoming prestige association environment for human survival.
In order to meet continuous improvement of the public to environmental quality requirement, country has worked out increasingly stricter discharge mark to nitrogen
Standard, research and development are economical, efficiently denitrogenate treatment technology has become emphasis and the focus of Water Pollution Control Engineering area research.Though
Many methods are so had effectively to remove ammonia, such as physical method has reverse osmosiss, distillation, soil irrigation；Chemical method has ion to hand over
Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysiss；Biological method has nitrification and algae
Class is cultivated, but physical method treatment effect is not good, and than chemical method, biological method processes waste water and has the following advantages:1) every kind of
The very strong product of being both for property of chemical article, is possible to failure, and biological preparation is to dirt when other chemical substances are run into
The removal of dye thing has light spectrality；2) chemical productss can temporarily eliminate some harmful substances and cover stink, lack and can not hinder
The only generation of harmful substance；3) using after chemical productss, residual is had in water body, may cause secondary pollution.Biological preparation institute
Containing natural microbial, without pathogenic bacterium and pathogen, these microorganisms under the catalytic action of enzyme, with the organic nutrition in sewage
Material is food, and after sewage is purified, these microbes are gradually decreased with the reduction of pollutant, until withering away；4)
Nontoxic, non-corrosiveness, easy to use, equipment or engineering need not be added substantially, save fund input.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients complexity, particularly substantial amounts of artificial conjunction
Compound is become to enter environment, this kind of material is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, as these materials itself are tied
The complexity of structure, can not be decomposed by the microorganisms utilization at short notice, and traditional method of wastewater treatment is tamed and dociled with activated sludge culture
The microorganism of change effectively can not be removed to these pollutant, and these materials are accumulated for a long time in the environment, relied to us
Very big pollution is caused with the ecological environment of existence, the physical and mental health for giving people class brings very big harm.Quite a few industry of China
Contaminating enterprises would rather be punished also and be unwilling that waste water is administered in investment, even if there is sewage-treatment plant operation also abnormal.Therefore, open
Sending out a kind of, construction investment is few, operating cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Content of the invention
In order to overcome the deficiencies in the prior art, effectively simply remove ammonia nitrogen sulfur phosphorus and heavy metal in sewage etc. and pollute
Thing, the invention provides a kind of method for removing dirty water pollutant, its technical scheme is realized by the following method:
A kind of method for removing mercurous wastewater pollutants, which comprises the steps:
The preparation of composite bacteria agent capable:By mixed bacteria liquid and carrier according to 1:1 weight is stirred than mixing, then stands 6
Hour, cold drying at most rearmounted 4 DEG C, after drying, water content control is obtained final product 6%；The carrier by bamboo charcoal, shitosan and
Kieselguhr is according to 2:2:1 mass ratio is mixed to get, particle diameter preferably 10 mesh of bamboo charcoal；
Sewage disinfection treatment:First by sewage through solid-liquid separator, solid-liquid separation is carried out, remove bulk solid particulate matter
Matter, subsequent liquid enters sedimentation tank, precipitates 12 hours, then liquid is removed solid floccules, circular hole mistake by circular hole drainage screen
The Circularhole diameter of filter screen is 0.1mm, biological oxidation:Liquid through circular hole drainage screen enters biological reaction pool, and it is 7 to adjust pH, presses
Per cubic metre of liquid adds 10 grams of composite bacteria agent capable every time, adds daily 1 time, continuously adds one week, finally stands 3 days, by liquid
Discharge.
Preferably, the composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
10 parts of Rhodococcus fascians, 9 parts of thiobacillus denitrificanss, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, Bacillus pumilus
5 parts, 2 parts of Phanerochaete chrysosporium；The concentration of above-mentioned each raw material bacterium all controls in (1-2) × 108Individual/ml.Above-mentioned strain is permissible
It is the conventional bacterial strain of prior art,
Preferably,
The Rhodococcus fascians are Rhodococcus fascians (Rhodococcusrhodochrous) ATCC15906；
The thiobacillus denitrificanss are thiobacillus denitrificanss (Thiobacillus denitrificans) ATCC25259；
The Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107；
The Sphingomonas are Sphingomonas (Sphingomonas sp.) CGMCC N0.4589；
The Bacillus pumilus are Bacillus pumilus (Bacillus pumilus) ATCC27142；
The Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium)
ATCC24725.
Bacterium of the present invention all can be from the business such as CGMCC, CCTCC and American Type Culture collection warehousing (ATCC)
Approach is commercially available.
Preferably, the Sphingomonas have proceeded to the sphingol of the encoding gene of coding any one protein variant following
Sporangium (Sphingomonas sp.) CGMCC N0.4589, the protein variant be with respect to PPKL1 albumen Original amino
Sequence be mutated as follows respectively 21L/Q, 59F/S, 75R/V, 121E/D, 165A/P, 199H/R, 242D/V, 421L/Y,
480A/S, 544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V, albumen original amino acid referring to
GenBank：NP_744926.1.
Present invention simultaneously provides a kind of transgenic strain for being capable of efficient absorption hydrargyrum, the bacterial strain is by by aminoacid sequence such as
Genbank：Sequence shown in NP_744926.1 is imported in Sphingomonas realizes its purpose.
And described aminoacid sequence can have a mutation in no site as follows, 21L/Q, 59F/S, 75R/V,
121E/D、165A/P、199H/R、242D/V、421L/Y、480A/S、544R/I、602A/L、690N/Q、729T/G、842A/G、
901Y/V.(21L/Q represents that the L amino acid substitution in original series the 21st is Q aminoacid).Aminoacid sequence after these mutation
Row are not willing the bacterial strain of the aminoacid sequence after having imported the mutation and equally have similar effect.And applicant is by substantial amounts of reality
Checking is real, and not all replacements all has similar effect, the Sphingomonas having after substantial amounts of replacement and other
Bacterial strain does not have the effect of preferable sewage disposal together.
Using DNAMAN software Design primers, BamHI and SalI restriction enzyme site is separately added into, complete according to aminoacid sequence
Nucleotide sequence PDA gene described in gene chemical synthesis, obtains purpose fragment by carrying out amplification, and PCR amplification obtains genes of interest
PDA (while by multiplex PCR, corresponding mutational site is incorporated in gene order, so as to obtain different mutants base
Cause), double digestion PCR primer is carried out with BamHI and SalI, with the expression vector pBPSE for also passing through BamHI and SalI double digestion
It is connected, the recombinant plasmid transformed being proved to be successful is entered in the Sphingomonas, obtains the genetic engineering bacterium of degraded hydrargyrum.
The amplification culture of each strain of the present invention is the cellar culture mode of this area, it is also possible to described in reference literature
Training method obtain.
What the present invention was obtained mainly has the beneficial effect that:The present invention processes sewage using pure microbial inoculum, has effectively purified dirt
Water, and dephosphorization, denitrogenation are while remove, the heavy metal Hg that can remove sewage Central China on this basis solves traditional work simultaneously
In skill, dephosphorization is difficult to denitrification effect while take into account the contradiction of heavy metals removal, with preferable application prospect.
Specific embodiment
Embodiment 1
A kind of method for removing dirty water pollutant, which comprises the steps:
The preparation of composite bacteria agent capable:By mixed bacteria liquid and carrier according to 1:1 weight is stirred than mixing, then stands 6
Hour, cold drying at most rearmounted 4 DEG C, after drying, water content control is obtained final product 6%；Carrier is by bamboo charcoal, shitosan and diatom
Soil is according to 2:2:1 mass ratio is mixed to get；Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight portion:Rhodococcus fascians
10 parts, 9 parts of thiobacillus denitrificanss, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of Bacillus pumilus, yellow archespore hair is put down
2 parts of lead fungi；The concentration of above-mentioned each raw material bacterium all controls 1 × 108Individual/ml.
Sewage disinfection treatment:It 300mg/L, sulfide 80mg/L, is 70mg/L containing phosphor contaminant by sewage NH3-N to be first,
Hg content is 20mg/ml, through solid-liquid separator, carries out solid-liquid separation, removes bulk solid particulate matter, and subsequent liquid is entered
Sedimentation tank, precipitates 12 hours, then liquid is removed solid floccules by circular hole drainage screen, and the Circularhole diameter of circular hole drainage screen is
0.1mm,
Biological oxidation:Liquid through circular hole drainage screen enters biological reaction pool, and it is 7.0 to adjust pH, by per cubic metre of liquid
Add 10 grams of composite bacteria agent capable every time, add I time daily, continuously add one week, finally 3 days are stood, liquid is discharged.After testing,
The content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, is 2.5mg/L containing phosphor contaminant, and clearance all reaches
More than 95%；And Hg content is that 15mg/mL, removal effect is not notable.
Embodiment 2
Using DNAMAN software Design primers, BamHI and SalI restriction enzyme site is separately added into, complete according to aminoacid sequence
Nucleotide sequence PDA gene described in gene chemical synthesis, obtains purpose fragment by carrying out amplification, and PCR amplification obtains genes of interest
PDA (while by multiplex PCR by corresponding mutational site 21L/Q, 59F/S, 75R/V, 121E/D, 165A/P, 199H/R,
242D/V, 421L/Y, 480A/S, 544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V are incorporated into gene order
In, so as to obtain different mutants gene), carry out double digestion PCR primer with BamHI and SalI, with also pass through BamHI and
The cloning vector pBPSE of SalI double digestion is connected, and the recombinant plasmid transformed being proved to be successful is entered into the sphingol list
In spore bacterium CGMCC N0.4589, the genetic engineering bacterium of degraded hydrargyrum is obtained.
The wastewater treatment efficiency of 3 Sphingomonas genetic engineering bacterium of embodiment and remaining several microbial inoculum is verified
According to the method for embodiment 1, corresponding sewage disposal experiment is carried out, its sewage belongs to same with the sewage of embodiment 1
One batch, the pollutant levels with same concentrations.The each group of wherein microbial inoculum is grouped into identical with embodiment 1.
The genetic engineering bacterium for being respectively adopted the different mutational site that embodiment 2 is prepared is removed the reaction of hydrargyrum,
Be found through experiments, 21L/Q, 59F/S, 75R/V, 121E/D, 165A/P, 199H/R, 242D/V, 421L/Y, 480A/S,
This several mutant bacteria of 544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V all have with respect to original bacteria have bright
Show enhanced effect.Total processing time is not more than 4 days, and its result is as follows：Sewage be with a collection of sewage, so as to guaranteed conditions one
Cause.
Each pollutant levels of sewage：NH3-N is 300mg/L, sulfide 80mg/L, is 70mg/L, Hg containing phosphor contaminant
Content is 20mg/ml.
As can be seen from the above results, the Sphingomonas for being built using genetic engineering are constituted with other several bacterial strains
Microbial inoculum also has, in addition to the original preferable effect for removing ammonia nitrogen, sulfide and phosphorus, the effect for preferably removing Hg
Really, and go back between engineered Sphingomonas and other several bacterial strains in terms of ammonia nitrogen, sulfide and phosphorus is removed
With enhanced synergism.
Claims (4)
1. a kind of method for removing mercurous wastewater pollutants, which comprises the steps:First by sewage through solid-liquid separator, enter
Row solid-liquid separation, removes bulk solid particulate matter, and subsequent liquid enters sedimentation tank, precipitates 12 hours, then by liquid by circle
Hole drainage screen removes solid floccules, and the liquid through circular hole filter screen filtration enters biological reaction pool, and it is 7 to adjust PH, by often standing
Square rice liquid adds 10 grams of composite bacteria agent capable every time, adds daily 1 time, continuously adds one week, finally stands 3 days, liquid is discharged.
2. the method for claim 1, it is characterised in that：The composite bacteria agent capable is by the raw material bacterium mixing of following weight portion
Become:10 parts of Rhodococcus fascians, 9 parts of thiobacillus denitrificanss, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingol single-cell, 5 parts of Bacillus pumilus,
2 parts of Phanerochaete chrysosporium；The concentration of above-mentioned each raw material bacterium all controls in (1-2) × 108Individual/ml.
3. method as claimed in claim 2, it is characterised in that：The Sphingomonas proceeded to coding following any one
Sphingol single-cell (Sphingomonas sp.) the CGMCC N0.4589 of the encoding gene of protein variant, the protein variant
Be with respect to PPKL1 albumen original amino acid be mutated as follows respectively 59F/S, 75R/V, 121E/D, 165A/P,
199H/R, 242D/V, 421L/Y, 480A/S, 544R/I, 602A/L, 690N/Q, 729T/G, 842A/G, 901Y/V, proteinogen
Beginning, aminoacid sequence was referring to GenBank：NP_744926.1.
4. a kind of protein variant, it is characterised in that：The protein variant is to be carried out with respect to albumen original amino acid respectively
Following mutation: 59F/S、75R/V、121E/D、165A/P、199H/R、242D/V、421L/Y、480A/S、544R/I、602A/
L, 690N/Q, 729T/G, 842A/G, 901Y/V, albumen original amino acid is referring to GenBank：NP_744926.1.
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| CN201710101117.2A Pending CN106830530A (en) | 2015-07-26 | 2015-07-26 | A kind of method for removing mercurous wastewater pollutants |
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| CN201710101100.7A Pending CN106830527A (en) | 2015-07-26 | 2015-07-26 | A kind of method for removing mercurous wastewater pollutants |
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| CN201710101109.8A Active CN106746370B (en) | 2015-07-26 | 2015-07-26 | Method for removing mercury-containing sewage pollutants |
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| CN106830359A (en) | 2017-06-13 |
| CN106830529A (en) | 2017-06-13 |
| CN106830530A (en) | 2017-06-13 |
| CN106830359B (en) | 2021-11-09 |
| CN106746370A (en) | 2017-05-31 |
| CN106830526A (en) | 2017-06-13 |
| CN106745823A (en) | 2017-05-31 |
| CN105130101A (en) | 2015-12-09 |
| CN106746370B (en) | 2020-12-11 |
| CN106830529B (en) | 2020-12-18 |
| CN106830527A (en) | 2017-06-13 |
| CN106830526B (en) | 2020-12-11 |
| CN106746371A (en) | 2017-05-31 |
| CN106745824B (en) | 2020-12-01 |
| CN105130101B (en) | 2017-05-24 |
| CN106830360B (en) | 2020-12-18 |
| CN106746371B (en) | 2021-10-22 |
| CN106830360A (en) | 2017-06-13 |
| CN106865781A (en) | 2017-06-20 |
| CN106745824A (en) | 2017-05-31 |
| CN106746369B (en) | 2021-11-05 |
| CN106865781B (en) | 2021-08-20 |
| CN106745823B (en) | 2020-12-11 |
| CN106830528A (en) | 2017-06-13 |
| CN106746369A (en) | 2017-05-31 |
| CN106430865B (en) | 2019-07-12 |
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Denomination of invention: A method for removing mercury containing wastewater pollutants Granted publication date: 20190712 Pledgee: China Everbright Bank Co.,Ltd. Lanzhou Branch Pledgor: ZHANGYE LANBIAO BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980002984 |