CN106587506B - A method of removal wastewater pollutants containing arsenic - Google Patents

A method of removal wastewater pollutants containing arsenic Download PDF

Info

Publication number
CN106587506B
CN106587506B CN201611187827.3A CN201611187827A CN106587506B CN 106587506 B CN106587506 B CN 106587506B CN 201611187827 A CN201611187827 A CN 201611187827A CN 106587506 B CN106587506 B CN 106587506B
Authority
CN
China
Prior art keywords
liquid
parts
solid
adds
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201611187827.3A
Other languages
Chinese (zh)
Other versions
CN106587506A (en
Inventor
李娜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hubei Kang Chuang Technology Co., Ltd.
Original Assignee
Hubei Kang Chuang Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hubei Kang Chuang Technology Co Ltd filed Critical Hubei Kang Chuang Technology Co Ltd
Priority to CN201611187827.3A priority Critical patent/CN106587506B/en
Publication of CN106587506A publication Critical patent/CN106587506A/en
Application granted granted Critical
Publication of CN106587506B publication Critical patent/CN106587506B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F2001/007—Processes including a sedimentation step
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/103—Arsenic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds

Abstract

The present invention relates to a kind of methods for removing the wastewater pollutants containing arsenic, include the following steps: first by sewage by solid-liquid separator, it is separated by solid-liquid separation, remove bulk solid particulate matter, subsequent liquid enters sedimentation basin, precipitating 12 hours, liquid is removed into solid floccules by circular hole filter screen again, liquid through circular hole filter screen filtration enters biological reaction pool, and adjusting PH is 7, adds 10 grams of composite bacteria agent every time by every cubic metre of liquid, it adds 1 time daily, it continuously adds one week, finally stands 3 days, liquid is discharged.Method of the invention can be effectively removed the pollutant containing ammonia nitrogen sulphur phosphorus and arsenic, and input cost is low, have preferable application prospect.

Description

A method of removal wastewater pollutants containing arsenic
Technical field
The invention belongs to environmental technology fields, and in particular to a method of remove dirty water pollutant.
Background technique
Along with industrial development and the improvement of people's living standards, trade effluent amount and city domestic sewage Amount just surges at an amazing speed, these sewage or or is polluting river, river and lake for the survival of mankind, structure One of the reason of assisting environment for human survival at prestige.
In order to meet continuous improvement of the public to environmental quality requirement, country has worked out the discharge mark being increasingly stringenter to nitrogen Standard, research and development are economical, efficiently denitrogenate emphasis and hot spot that processing technique has become Water Pollution Control Engineering area research.Though It so can be effectively removed ammonia there are many method, as physical method has reverse osmosis, distillation, soil to irrigate；Chemical method has ion friendship Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysis；Biological method has nitrification and algae Class cultivation, however physical method treatment effect is bad, compared with chemical method, biological method processing waste water has the following advantages: 1) every kind Chemical article is possible to fail both for the very strong product of property when encountering other chemical substances, and biological agent is to dirt The removal for contaminating object has spectrum；2) chemical products can temporarily eliminate certain harmful substances and cover stink, and lacking cannot hinder The only generation of harmful substance；3) using after chemical products, residual is had in water body, may cause secondary pollution.Biological agent institute Containing natural microbial, pathogenic bacteria and pathogen are free of, these microorganisms are under the catalytic action of enzyme, with the organic nutrition in sewage Substance is food, and after purifying the sewage, these microbes are gradually decreased with the reduction of pollutant, until withering away；4) It is nontoxic, it is non-corrosive, it is easy to use, addition equipment or engineering are not needed substantially, save capital investment.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients is complicated, especially a large amount of artificial conjunction Enter environment at compound, this substance is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, since these substances itself are tied The complexity of structure, cannot be decomposed by the microorganisms utilization in a short time, and traditional wastewater treatment method is tamed and dociled with activated sludge culture The microorganism of change cannot effectively remove these pollutants, these substances accumulate in the environment for a long time, rely to us Very big pollution is caused with the ecological environment of existence, brings very big harm to the physical and mental health of the mankind.Quite a few industry of China Contaminating enterprises, which would rather be punished, is also unwilling that waste water is administered in investment, abnormal having sewage-treatment plant operation.Therefore, it opens Sending out a kind of, construction investment is few, operating cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Summary of the invention
For overcome the deficiencies in the prior art, the pollution such as ammonia nitrogen sulphur phosphorus and heavy metal in sewage is effectively simply removed Object, the present invention provides a kind of method for removing dirty water pollutant, technical solution is realized by the following method:
A method of removal wastewater pollutants containing arsenic comprising following steps:
The preparation of composite bacteria agent: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs evenly, is then allowed to stand 6 Hour, low temperature drying at most 4 DEG C of postposition, it is dry after water content control 6% to get；The carrier by bamboo charcoal, chitosan and Diatomite is mixed to get according to the mass ratio of 2:2:1, the partial size of bamboo charcoal preferably 10 mesh；
Sewage disinfection treatment: passing through solid-liquid separator for sewage first, be separated by solid-liquid separation, and removes bulk solid particulate matter Matter, subsequent liquid enter sedimentation basin, precipitate 12 hours, then liquid is removed solid floccules, circular hole mistake by circular hole filter screen The Circularhole diameter of strainer is 0.1mm, and biological oxidation: the liquid through circular hole filter screen enters biological reaction pool, and adjusting pH is 7, is pressed Every cubic metre of liquid adds 10 grams of composite bacteria agent every time, adds I times daily, continuously adds one week, finally 3 days is stood, by liquid Discharge.
Preferably, the composite bacteria agent is mixed by the raw material bacterium of following parts by weight:
10 parts of Rhodococcus sp, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, bacillus pumilus 5 parts, 2 parts of Phanerochaete chrysosporium；The concentration of above-mentioned each raw material bacterium is controlled in (1-2) X108A/ml.Above-mentioned strain can be The conventional bacterial strain of the prior art,
Preferably,
The Rhodococcus sp is Rhodococcus sp (Rhodococcusrhodochrous) ATCC15906；
The thiobacillus denitrificans are thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259；
The Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107；
The Sphingomonas is Sphingomonas (Sphingomonas sp.) CGMCC N0.4589；
The bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142；
The yellow flat lead fungi of raw wool of embracing is yellow armful the flat lead fungi of raw wool (Phanerochaete chrysosporium) ATCC24725。
Bacterium of the present invention can be from the business such as CGMCC, CCTCC and American Type Culture collection warehousing (ATCC) Approach is commercially available.
Preferably, the Rhodococcus sp is the ATCC15906 for being transferred to the encoding gene for encoding any one following protein variant Bacterial strain, the protein variant be carry out being mutated as follows respectively relative to albumen original amino acid 62K/G, 80E/V, 93V/H, 120R/I, 131F/P, 143Q/S, 152P/Q, 169G/I, 179Q/A, 192Q/G, 241W/S, 258R/T, 263R/P, proteinogen Beginning, amino acid sequence was referring to GenBank:NP_745067.1.
Be capable of the transgenic strain of efficient absorption arsenic present invention simultaneously provides one kind, the bacterial strain by by amino acid sequence such as Sequence shown in Genbank:NP_745067.1, which is imported into Rhodococcus sp, realizes its purpose.
And the amino acid sequence can have the mutation in following unused site, 62K/G, 80E/V, 93V/H, 120R/I、131F/P、143Q/S、152P/Q、169G/I、179Q/A、192Q/G、241W/S、258R/T、263R/P。(62K/G Indicate that in original series the 62nd K amino acid substitution be G amino acid).Amino acid sequence after these mutation does not grudge importing The bacterial strain of amino acid sequence after the mutation equally has similar effect.And applicant confirms through a large number of experiments, not That all substitutions all have similar effect, have the Rhodococcus sp after a large amount of replace together with other bacterial strains and do not have compared with The effect of good sewage treatment.
Using DNAMAN software Design primers, it has been separately added into restriction enzyme site, it is complete according to amino acid sequence NP_745067.1 Nucleotide sequence PHY gene described in gene chemical synthesis obtains target fragment by carrying out amplification, and PCR amplification obtains target gene PHY (while corresponding mutational site being introduced into gene order by multiplex PCR, to obtain different mutants base Cause), double digestion PCR product is carried out with restriction endonuclease, is connected with the expression vector pEASY for also passing through double digestion, will be proved to be successful Recombinant plasmid transformed enter in the Rhodococcus sp, obtain degradation arsenic genetic engineering bacterium.
The expansion culture of each strain of the invention is the routine culture mode of this field, is also referred to record in document Training method obtain.
What the present invention obtained mainly has the beneficial effect that: the present invention handles sewage using pure microbial inoculum, has effectively purified dirt Water, and dephosphorization, denitrogenation remove simultaneously, and the heavy metal arsenic that can remove sewage Central China simultaneously on this basis solves traditional work Dephosphorization and denitrification effect are difficult to combine the contradiction of heavy metals removal in skill, with good application prospect.
Specific embodiment
Embodiment 1
A method of removing dirty water pollutant comprising following steps:
The preparation of composite bacteria agent: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs evenly, is then allowed to stand 6 Hour, low temperature drying at most 4 DEG C of postposition, it is dry after water content control 6% to get；Carrier is by bamboo charcoal, chitosan and diatom Soil is mixed to get according to the mass ratio of 2:2:1；Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following parts by weight: Rhodococcus sp 10 parts, 9 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of bacillus pumilus, yellow archespore hair is flat 2 parts of lead fungi；The concentration of above-mentioned each raw material bacterium is controlled 1 × 108A/ml.
Sewage disinfection treatment: being first 300mg/L, sulfide 80mg/L by sewage NH3-N, be 70mg/L containing phosphor contaminant, Arsenic content is 20mg/ml, by solid-liquid separator, is separated by solid-liquid separation, and bulk solid particulate matter is removed, and subsequent liquid enters Sedimentation basin precipitates 12 hours, then liquid is removed solid floccules by circular hole filter screen, and the Circularhole diameter of circular hole filter screen is 0.1mm,
Biological oxidation: the liquid through circular hole filter screen enters biological reaction pool, and adjusting pH is 7.0, by every cubic metre of liquid 10 grams of composite bacteria agent are added every time, is added daily 1 time, is continuously added one week, is finally stood 3 days, liquid is discharged.Through detecting, The content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, is 2.5mg/L containing phosphor contaminant, and removal rate reaches 96% or more；And arsenic content is 17mg/mL, removal effect is not significant.
Embodiment 2
Using DNAMAN software Design primers, it has been separately added into restriction enzyme site, it is complete according to amino acid sequence NP_745067.1 Nucleotide sequence PHY gene described in gene chemical synthesis obtains target fragment by carrying out amplification, and PCR amplification obtains target gene PHY (while by multiplex PCR by corresponding mutational site 62K/G, 80E/V, 93V/H, 120R/I, 131F/P, 143Q/S, 152P/Q, 169G/I, 179Q/A, 192Q/G, 241W/S, 258R/T, 263R/P are introduced into gene order, to obtain not Same mutant gene), double digestion PCR product is carried out with the restriction enzyme site of addition, is carried with the clonal expression for also passing through double digestion Body pEASY is connected, and the recombinant plasmid transformed being proved to be successful is entered in the Rhodococcus sp ATCC15906 bacterial strain, is degraded The genetic engineering bacterium of arsenic.
The wastewater treatment efficiency of 3 Rhodococcus genes engineering bacteria of embodiment and remaining several microbial inoculum is verified
According to the method for embodiment 1, corresponding sewage treatment experiment is carried out, sewage and the sewage of embodiment 1 belong to together One batch, the pollutant concentration with same concentrations.Wherein each group of microbial inoculum is grouped as identical with embodiment 1.
The genetic engineering bacterium that different mutational site as described in example 2 is respectively adopted is removed the reaction of arsenic, passes through Experiment discovery, 62K/G, 80E/V, 93V/H, 120R/I, 131F/P, 143Q/S, 152P/Q, 169G/I, 179Q/A, 192Q/G, 241W/S, 258R/T, 263R/P these types mutant bacteria, which all have the effect of having relative to original bacteria, to be remarkably reinforced.When total processing Between be not more than 4 days, result is as follows: sewage is with a collection of sewage, so that guaranteed conditions are consistent.
Each pollutant concentration of sewage: NH3-N 300mg/L, sulfide 80mg/L are 70mg/L, arsenic containing phosphor contaminant Content is 20mg/ml.
As can be seen from the above results, it is removed using the microbial inoculum that the Rhodococcus sp that genetic engineering constructs is constituted with other several bacterial strains Have the effect of also having the effect of preferably going arsenic removal except original preferable removal ammonia nitrogen, sulfide and phosphorus, and Also there is cooperateing with for enhancing between the Rhodococcus sp and other several bacterial strains of genetic engineering in terms of removal ammonia nitrogen, sulfide and the phosphorus Effect.

Claims (2)

1. a kind of method for removing the wastewater pollutants containing arsenic comprising following steps: sewage is passed through into solid-liquid separator first, into Row is separated by solid-liquid separation, and removes bulk solid particulate matter, and subsequent liquid enters sedimentation basin, is precipitated 12 hours, then liquid is passed through circle Hole filter screen removes solid floccules, and the liquid through circular hole filter screen filtration enters biological reaction pool, and adjusting pH is 7, by often standing Square rice liquid adds 10 grams of composite bacteria agent every time, adds I times daily, continuously adds one week, finally stands 3 days, liquid is discharged, The composite bacteria agent is mixed by the raw material bacterium of following parts by weight: 10 parts of Rhodococcus sp, 9 parts of thiobacillus denitrificans, Amur is false single 7 parts of born of the same parents bacterium, 6 parts of Sphingol single-cell, 5 parts of bacillus pumilus, 2 parts of Phanerochaete chrysosporium；The concentration of above-mentioned each raw material bacterium Control is in (1-2) X108A/ml, the Rhodococcus sp are the encoding gene for being transferred to the following protein variant of coding ATCC15906 bacterial strain, the protein variant are to be mutated as follows relative to albumen original amino acid: 80E/V, proteinogen Beginning, amino acid sequence was referring to GenBank:NP_745067.1.
2. a kind of protein variant, it is characterised in that: the protein variant is as follows relative to the progress of albumen original amino acid Mutation: 80E/V, albumen original amino acid is referring to GenBank:NP_745067.1.
CN201611187827.3A 2015-07-26 2015-07-26 A method of removal wastewater pollutants containing arsenic Active CN106587506B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611187827.3A CN106587506B (en) 2015-07-26 2015-07-26 A method of removal wastewater pollutants containing arsenic

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201611187827.3A CN106587506B (en) 2015-07-26 2015-07-26 A method of removal wastewater pollutants containing arsenic
CN201510441623.7A CN105060620B (en) 2015-07-26 2015-07-26 It is a kind of to remove the method containing arsenic wastewater pollutants

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201510441623.7A Division CN105060620B (en) 2015-07-26 2015-07-26 It is a kind of to remove the method containing arsenic wastewater pollutants

Publications (2)

Publication Number Publication Date
CN106587506A CN106587506A (en) 2017-04-26
CN106587506B true CN106587506B (en) 2019-07-05

Family

ID=54490210

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201510441623.7A Active CN105060620B (en) 2015-07-26 2015-07-26 It is a kind of to remove the method containing arsenic wastewater pollutants
CN201611187827.3A Active CN106587506B (en) 2015-07-26 2015-07-26 A method of removal wastewater pollutants containing arsenic

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201510441623.7A Active CN105060620B (en) 2015-07-26 2015-07-26 It is a kind of to remove the method containing arsenic wastewater pollutants

Country Status (1)

Country Link
CN (2) CN105060620B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09314185A (en) * 1996-05-30 1997-12-09 Ebara Corp Method and apparatus for treating organic sewage
CN101050030A (en) * 2007-03-21 2007-10-10 李昕 Method for treating arsenic waste solution of hazardous waste by using composite functional bacteria
CN103449680A (en) * 2013-09-13 2013-12-18 李丽萍 Method for removing pollutants in sewage

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103449681B (en) * 2013-09-13 2014-11-26 何洋 Method for treating sewage by using microbial preparation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09314185A (en) * 1996-05-30 1997-12-09 Ebara Corp Method and apparatus for treating organic sewage
CN101050030A (en) * 2007-03-21 2007-10-10 李昕 Method for treating arsenic waste solution of hazardous waste by using composite functional bacteria
CN103449680A (en) * 2013-09-13 2013-12-18 李丽萍 Method for removing pollutants in sewage

Also Published As

Publication number Publication date
CN106587506A (en) 2017-04-26
CN105060620B (en) 2017-05-31
CN105060620A (en) 2015-11-18

Similar Documents

Publication Publication Date Title
CN105036471B (en) A kind of method for removing leaded wastewater pollutants
CN105130100B (en) It is a kind of to remove the method containing cadmium wastewater pollutants
CN106007243B (en) A method of removal high concentration chrome wastewater pollutant
CN106430865B (en) A method of removing mercurous wastewater pollutants
CN106587506B (en) A method of removal wastewater pollutants containing arsenic

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20190610

Address after: 432000 Three Li village, Changjiang port office, Yingcheng City, Xiaogan, Hubei

Applicant after: Hubei Kang Chuang Technology Co., Ltd.

Address before: 100193 China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing

Applicant before: Li Na

GR01 Patent grant
GR01 Patent grant