CN106587506A - Method for removing pollutants from sewage containing arsenic - Google Patents
Method for removing pollutants from sewage containing arsenic Download PDFInfo
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- CN106587506A CN106587506A CN201611187827.3A CN201611187827A CN106587506A CN 106587506 A CN106587506 A CN 106587506A CN 201611187827 A CN201611187827 A CN 201611187827A CN 106587506 A CN106587506 A CN 106587506A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/103—Arsenic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Abstract
The invention relates to a method for removing pollutants from sewage containing arsenic, and the method includes the following steps: first, the sewage is subjected to solid-liquid separation by a solid-liquid separator so as to remove large solid particles; then, the liquid flows into a sedimentation tank and is subjected to sedimentation for 12 hours, a solid flocculate is removed from the liquid through a round hole filter net, the filtered liquid through the round hole filter net enters a biological reaction tank, pH value is adjusted to 7, 10 grams of compound microbial inoculum are added to each cubic meter of liquid each time, wherein addition of the compound microbial inoculum is conducted once a day and continuously conducted for a week, and finally the materials are allowed to stand for 3 days, and the liquid is discharged. The method of the invention can efficiently remove the pollutants containing ammonia, nitrogen, sulfur, phosphorus and arsenic, and is low in investment cost and has good application prospect.
Description
Technical field
The invention belongs to environmental technology field, and in particular to a kind of method of removal dirt water pollutant.
Background technology
Along with industrial development and the improvement of people's living standards, trade effluent amount and city domestic sewage
Amount just surges with surprising rapidity, these sewage or or has polluted river for the survival of mankind, river and lake, structure
One of the reason for environment for human survival being assisted into prestige.
In order to meet continuous improvement of the public to environmental quality requirement, country has worked out increasingly stricter discharge mark to nitrogen
Standard, research and development are economical, efficiently denitrogenate treatment technology has become the emphasis and focus of Water Pollution Control Engineering area research.Though
So there are many methods effectively to remove ammonia, such as physical method has reverse osmosiss, distillation, soil irrigation;Chemical method has ion to hand over
Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysiss;Biological method has nitrification and algae
Class is cultivated, but physical method treatment effect is not good, and than chemical method, biological method processes waste water and has the following advantages:1) it is every kind of
The very strong product of being both for property of chemical article, failure is possible to when other chemical substances are run into, and biological preparation is to dirt
The removal of dye thing has light spectrality;2) chemical productss can temporarily eliminate some harmful substances and cover stink, and lacking to hinder
The only generation of harmful substance;3) after using chemical productss, residual is had in water body, secondary pollution may be caused.Biological preparation institute
Containing natural microbial, without pathogenic bacterium and pathogen, these microorganisms under the catalytic action of enzyme, with the organic nutrition in sewage
Material is food, and after sewage is purified, these microbes are gradually decreased with the reduction of pollutant, until withering away;4)
Nontoxic, non-corrosiveness is easy to use, and equipment or engineering need not be added substantially, saves fund input.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients is complicated, particularly substantial amounts of artificial conjunction
Environment is entered into compound, this kind of material is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, due to these materials knot itself
The complexity of structure, can not be decomposed by the microorganisms at short notice utilization, and traditional method of wastewater treatment is tamed and dociled with activated sludge culture
The microorganism of change can not be removed effectively to these pollutant, and these materials are accumulated in the environment for a long time, be relied to us
Very big pollution is caused with the ecological environment survived, the physical and mental health for giving people class brings very big harm.Quite a few industry of China
Contaminating enterprises would rather be punished also be unwilling to invest and administer waste water, even if there is sewage-treatment plant operation also abnormal.Therefore, open
Sending out a kind of, construction investment is few, operating cost is low, good treatment efficiency sewage disposal technology is extremely urgent.
The content of the invention
In order to overcome the deficiencies in the prior art, effectively simply remove ammonia nitrogen sulfur phosphorus and heavy metal in sewage etc. and pollute
Thing, the invention provides a kind of method for removing dirty water pollutant, its technical scheme is realized by the following method:
A kind of to remove the method containing arsenic wastewater pollutants, it comprises the steps:
The preparation of composite bacteria agent capable:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, then stands 6
Hour, it is most rearmounted 4 DEG C at cold drying, be dried after water content control 6%, obtain final product;The carrier by bamboo charcoal, shitosan and
Kieselguhr is according to 2:2:1 mass ratio is mixed to get, particle diameter preferably 10 mesh of bamboo charcoal;
Sewage disinfection treatment:First by sewage through solid-liquid separator, solid-liquid separation is carried out, remove bulk solid particulate matter
Matter, subsequent liquid enters sedimentation tank, precipitates 12 hours, then liquid is removed into solid floccules, circular hole mistake by circular hole drainage screen
The Circularhole diameter of filter screen be 0.1mm, biological oxidation:The liquid of Jing circular hole drainage screens enters biological reaction pool, and it is 7 to adjust pH, is pressed
Every cubic metre of liquid adds every time 10 grams of composite bacteria agent capable, adds I time daily, continuously adds one week, finally 3 days is stood, by liquid
Discharge.
Preferably, the composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
10 parts of Rhodococcus fascians, 9 parts of thiobacillus denitrificanss, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, Bacillus pumilus
5 parts, 2 parts of Phanerochaete chrysosporium;The concentration of above-mentioned each raw material bacterium is controlled in (1-2) X108Individual/ml.Above-mentioned strain can be
The conventional bacterial strain of prior art,
Preferably,
The Rhodococcus fascians are Rhodococcus fascians (Rhodococcusrhodochrous) ATCC15906;
The thiobacillus denitrificanss are thiobacillus denitrificanss (Thiobacillus denitrificans) ATCC25259;
The Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107;
The Sphingomonas are Sphingomonas (Sphingomonas sp.) CGMCC N0.4589;
The Bacillus pumilus are Bacillus pumilus (Bacillus pumilus) ATCC27142;
The yellow flat lead fungi of raw wool of embracing embraces the flat lead fungi of raw wool (Phanerochaete chrysosporium) for Huang
ATCC24725。
Bacterium of the present invention can be from the business such as CGMCC, CCTCC and American Type Culture collection warehousing (ATCC)
Approach is commercially available.
Preferably, the Rhodococcus fascians are the ATCC15906 of the encoding gene for having proceeded to following any one protein variant of coding
Bacterial strain, the protein variant be relative to albumen original amino acid be mutated as follows respectively 62K/G, 80E/V, 93V/H,
120R/I, 131F/P, 143Q/S, 152P/Q, 169G/I, 179Q/A, 192Q/G, 241W/S, 258R/T, 263R/P, proteinogen
Beginning, aminoacid sequence was referring to GenBank:NP_745067.1.
Present invention simultaneously provides a kind of transgenic strain for being capable of efficient absorption arsenic, the bacterial strain by by aminoacid sequence such as
Genbank:Sequence shown in NP_745067.1 is imported to and realize in Rhodococcus fascians its purpose.
And described aminoacid sequence can have a mutation in following no site, 62K/G, 80E/V, 93V/H,
120R/I、131F/P、143Q/S、152P/Q、169G/I、179Q/A、192Q/G、241W/S、258R/T、263R/P。(62K/G
Represent that the K amino acid substitutions in original series the 62nd are G aminoacid).Aminoacid sequence after these mutation does not grudge importing
The bacterial strain of the aminoacid sequence after the mutation equally has similar effect.And applicant is by substantial amounts of it is experimentally confirmed that not
Be it is all of replacement all have similar effect, have the Rhodococcus fascians after substantial amounts of replacement together with other bacterial strains have compared with
The effect of good sewage disposal.
Using DNAMAN software Design primers, restriction enzyme site is separately added into, it is complete according to aminoacid sequence NP_745067.1
Nucleotide sequence PHY genes described in gene chemical synthesis, by carrying out amplification purpose fragment is obtained, and PCR amplifications obtain genes of interest
PHY (while corresponding mutational site is incorporated in gene order by multiplex PCR, so as to obtain different mutants base
Cause), double digestion PCR primer is carried out with restriction endonuclease, it is connected with the expression vector pEASY for also passing through double digestion, will be proved to be successful
Recombinant plasmid transformed enter in the Rhodococcus fascians, obtain degraded arsenic genetic engineering bacterium.
The amplification culture of each strain of the present invention is the cellar culture mode of this area, it is also possible to described in reference literature
Training method obtain.
What the present invention was obtained mainly has the beneficial effect that:The present invention processes sewage using pure microbial inoculum, has effectively purified dirt
Water, and dephosphorization, denitrogenation removal simultaneously, the heavy metal arsenic that can remove sewage Central China simultaneously on this basis solves traditional work
Dephosphorization and denitrification effect are difficult to while take into account the contradiction of heavy metals removal in skill, with preferable application prospect.
Specific embodiment
Embodiment 1
A kind of method for removing dirty water pollutant, it comprises the steps:
The preparation of composite bacteria agent capable:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, then stands 6
Hour, it is most rearmounted 4 DEG C at cold drying, be dried after water content control 6%, obtain final product;Carrier is by bamboo charcoal, shitosan and diatom
Soil is according to 2:2:1 mass ratio is mixed to get;Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight portion:Rhodococcus fascians
10 parts, 9 parts of thiobacillus denitrificanss, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of Bacillus pumilus, yellow archespore hair is put down
2 parts of lead fungi;The concentration of above-mentioned each raw material bacterium is controlled 1 × 108Individual/ml.
Sewage disinfection treatment:It is 300mg/L, sulfide 80mg/L first by sewage NH3-N, is 70mg/L containing phosphor contaminant,
Arsenic content is 20mg/ml, through solid-liquid separator, carries out solid-liquid separation, removes bulk solid particulate matter, and subsequent liquid is entered
Sedimentation tank, precipitates 12 hours, then liquid is removed into solid floccules by circular hole drainage screen, and the Circularhole diameter of circular hole drainage screen is
0.1mm,
Biological oxidation:The liquid of Jing circular hole drainage screens enters biological reaction pool, and it is 7.0 to adjust pH, by every cubic metre of liquid
10 grams of composite bacteria agent capable being added every time, being added daily 1 time, continuously added one week, finally stand 3 days, liquid is discharged.After testing,
The content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, is 2.5mg/L containing phosphor contaminant, and clearance reaches
More than 96%;And arsenic content is 17mg/mL, removal effect is not notable.
Embodiment 2
Using DNAMAN software Design primers, restriction enzyme site is separately added into, it is complete according to aminoacid sequence NP_745067.1
Nucleotide sequence PHY genes described in gene chemical synthesis, by carrying out amplification purpose fragment is obtained, and PCR amplifications obtain genes of interest
PHY (while by multiplex PCR by corresponding mutational site 62K/G, 80E/V, 93V/H, 120R/I, 131F/P, 143Q/S,
152P/Q, 169G/I, 179Q/A, 192Q/G, 241W/S, 258R/T, 263R/P are incorporated in gene order, so as to obtain not
Same mutant gene), double digestion PCR primer is carried out with the restriction enzyme site for adding, carry with the clonal expression for also passing through double digestion
Body pEASY is connected, and the recombinant plasmid transformed being proved to be successful is entered in the Rhodococcus fascians ATCC15906 bacterial strains, is degraded
The genetic engineering bacterium of arsenic.
The Rhodococcus genes engineering bacteria of embodiment 3 is verified with the wastewater treatment efficiency of remaining several microbial inoculum
According to the method for embodiment 1, corresponding sewage disposal experiment is carried out, its sewage belongs to same with the sewage of embodiment 1
One batch, the pollutant levels with same concentrations.The each group of wherein microbial inoculum is grouped into identical with embodiment 1.
The genetic engineering bacterium that the different mutational site described in embodiment 2 is respectively adopted is removed the reaction of arsenic, passes through
Experiment find, 62K/G, 80E/V, 93V/H, 120R/I, 131F/P, 143Q/S, 152P/Q, 169G/I, 179Q/A, 192Q/G,
241W/S, 258R/T, 263R/P this several mutant bacteria all have and have the effect being remarkably reinforced relative to original bacteria.During total process
Between be not more than 4 days, its result is as follows:Sewage is with a collection of sewage, so as to guaranteed conditions are consistent.
Each pollutant levels of sewage:NH3-N is 300mg/L, sulfide 80mg/L, is 70mg/L containing phosphor contaminant, arsenic
Content is 20mg/ml.
As can be seen from the above results, the microbial inoculum that the Rhodococcus fascians for being built using genetic engineering are constituted with other several bacterial strains is removed
Outside with the original preferable effect for removing ammonia nitrogen, sulfide and phosphorus, also with the effect for preferably going arsenic removal, and
Also there is enhanced collaboration in terms of ammonia nitrogen, sulfide and phosphorus is removed between engineered Rhodococcus fascians and other several bacterial strains
Effect.
Claims (4)
1. a kind of to remove the method containing arsenic wastewater pollutants, it comprises the steps:First by sewage through solid-liquid separator, enter
Row solid-liquid separation, removes bulk solid particulate matter, and subsequent liquid enters sedimentation tank, precipitates 12 hours, then by liquid by circle
Hole drainage screen removes solid floccules, and the liquid of Jing circular hole filter screen filtrations enters biological reaction pool, and it is 7 to adjust PH, by often standing
Square rice liquid adds every time 10 grams of composite bacteria agent capable, adds I time daily, continuously adds one week, finally stands 3 days, and liquid is discharged.
2. the method for claim 1, it is characterised in that:Described composite bacteria agent capable is mixed by the raw material bacterium of following weight portion
Form:10 parts of Rhodococcus fascians, 9 parts of thiobacillus denitrificanss, 7 parts of Pseudomonas stutzeri, 6 parts of Sphingol single-cell, Bacillus pumilus 5
Part, 2 parts of Phanerochaete chrysosporium;The concentration of above-mentioned each raw material bacterium is controlled in (1-2) X 108Individual/ml.
3. method as claimed in claim 2, it is characterised in that:The Rhodococcus fascians encode following any one albumen to have proceeded to
The ATCC15906 bacterial strains of the encoding gene of variant, the protein variant is to carry out respectively relative to albumen original amino acid
Following mutation: 80E/V、93V/H、120R/I、131F/P、143Q/S、152P/Q、169G/I、179Q/A、192Q/G、241W/
S, 258R/T, 263R/P, albumen original amino acid is referring to GenBank:NP_745067.1.
4. a kind of protein variant, it is characterised in that:The protein variant is to carry out respectively relative to albumen original amino acid
Following mutation: 80E/V、93V/H、120R/I、131F/P、143Q/S、152P/Q、169G/I、179Q/A、192Q/G、241W/
S, 258R/T, 263R/P, albumen original amino acid is referring to GenBank:NP_745067.1.
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CN201611187827.3A CN106587506B (en) | 2015-07-26 | 2015-07-26 | A method of removal wastewater pollutants containing arsenic |
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CN201510441623.7A CN105060620B (en) | 2015-07-26 | 2015-07-26 | It is a kind of to remove the method containing arsenic wastewater pollutants |
CN201611187827.3A CN106587506B (en) | 2015-07-26 | 2015-07-26 | A method of removal wastewater pollutants containing arsenic |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09314185A (en) * | 1996-05-30 | 1997-12-09 | Ebara Corp | Method and apparatus for treating organic sewage |
CN101050030A (en) * | 2007-03-21 | 2007-10-10 | 李昕 | Method for treating arsenic waste solution of hazardous waste by using composite functional bacteria |
CN103449680A (en) * | 2013-09-13 | 2013-12-18 | 李丽萍 | Method for removing pollutants in sewage |
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CN103449681B (en) * | 2013-09-13 | 2014-11-26 | 何洋 | Method for treating sewage by using microbial preparation |
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- 2015-07-26 CN CN201611187827.3A patent/CN106587506B/en active Active
- 2015-07-26 CN CN201510441623.7A patent/CN105060620B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09314185A (en) * | 1996-05-30 | 1997-12-09 | Ebara Corp | Method and apparatus for treating organic sewage |
CN101050030A (en) * | 2007-03-21 | 2007-10-10 | 李昕 | Method for treating arsenic waste solution of hazardous waste by using composite functional bacteria |
CN103449680A (en) * | 2013-09-13 | 2013-12-18 | 李丽萍 | Method for removing pollutants in sewage |
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CN105060620A (en) | 2015-11-18 |
CN105060620B (en) | 2017-05-31 |
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Effective date of registration: 20190610 Address after: 432000 Three Li village, Changjiang port office, Yingcheng City, Xiaogan, Hubei Applicant after: Hubei Kang Chuang Technology Co., Ltd. Address before: 100193 China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing Applicant before: Li Na |
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