CN103449681A - Method for treating sewage by using microbial preparation - Google Patents
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- CN103449681A CN103449681A CN2013104192260A CN201310419226A CN103449681A CN 103449681 A CN103449681 A CN 103449681A CN 2013104192260 A CN2013104192260 A CN 2013104192260A CN 201310419226 A CN201310419226 A CN 201310419226A CN 103449681 A CN103449681 A CN 103449681A
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- 241000611330 Chryseobacterium Species 0.000 claims description 10
- WWUZIQQURGPMPG-KRWOKUGFSA-N sphingosine Chemical compound CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](N)CO WWUZIQQURGPMPG-KRWOKUGFSA-N 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 8
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 8
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 229910021529 ammonia Inorganic materials 0.000 abstract description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract description 3
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- 231100000719 pollutant Toxicity 0.000 abstract description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
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- 238000011017 operating method Methods 0.000 abstract 1
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
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- 229920002472 Starch Polymers 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
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- 241000700605 Viruses Species 0.000 description 1
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- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
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- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
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Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of environmental protection and discloses a method for treating sewage by using a microbial preparation. The method comprises the steps of preparing the microbial preparation, pre-treating the sewage, carrying out microbial oxidation, and the like. The invention further discloses the microbial preparation for sewage treatment. The method disclosed by the invention has the advantages that the operating methods are simple, the investment is small, and pollutants, such as ammonia, nitrogen, sulfur, phosphorus and the like, in the sewage can be effectively removed.
Description
Technical field
The invention belongs to environmental technology field, be specifically related to a kind of method of utilizing microbial preparation to dispose of sewage.
Background technology
Sewage, be often referred to be subject to certain that pollute, from life and the waste water produced.Sewage mainly contains sanitary sewage and trade effluent.In the water pollution that the human production activity causes, the water pollution that industry causes is the most serious.As trade effluent, it is many containing pollutent, and complicated component not only is difficult for purifying, and processes also more difficult in water.Trade effluent is the most important reason that industrial pollution causes water pollution.It accounts for the major part of the pollutent of industry discharge.The contained pollutent of trade effluent varies because factory's kind is different, even same class factory, the production process difference, the quality and quantity of its contained pollutent is also different.The industrial waste water except discharge directly injects water body to be caused pollution, and solid waste and waste gas also can polluted-waters.Sanitary sewage is the sewage produced in human lives's process, is one of primary pollution source of water body.Mainly ight soil and wash water.The sanitary sewage amount that discharge everyone every day in city is 150-400L, and its amount has substantial connection with standard of living.Contain large amount of organic in sanitary sewage, as Mierocrystalline cellulose, starch, carbohydrate and fat protein etc.; Also often contain pathogenic bacteria, virus and parasitic ovum; The muriate of inorganic salts, vitriol, phosphoric acid salt, supercarbonate and sodium, potassium, calcium, magnesium etc.Total characteristics are nitrogenous, sulfur-bearing and phosphorous height, and post-processed is more difficult.People should water conservation. and China's actual conditions are more special, and water resources occupancy volume per person is few, the spatial distribution imbalance.Along with Chinese Urbanization's, industrialized acceleration, the demand gap of water resources also increases day by day.Under such background, sewage treatment industry becomes new industry, at present and tap water production, water supply, draining, middle water reuse industry in status of equal importance.
Sulphur phosphorus ammonia nitrogen is the principal pollutant in sewage, is also modal pollutent.The main method of the pollutents such as sulphur phosphorus ammonia nitrogen in prior art in removal sewage commonly used has: Physical, chemical method, biological process.Physical is containing treatment technologies such as reverse osmosis, distillation, soil irrigations; Chemical method is containing treatment technologies such as ion-exchange, ammonia stripping, break point chlorination, burning, chemical precipitation, catalytic pyrolysis, electrodialysis, electrochemistry; Biological process is containing treatment technologies such as algal culture, biological nitration, immobilized biotechnologies.Practical method has at present: break point chlorination method, selective ion exchange method, ammonia blow-off method, biological process and chemical precipitation method.Wherein, break point chlorination method, selective ion exchange method, ammonia blow-off method are not suitable for the large-scale industrialization application, and chemical process easily causes other pollutions, and cost is higher, and the method for the most economic environmental protection at present is microbial method.Prior art is in the urgent need to a kind of effectively dispose of sewage method of the pollutents such as middle ammonia nitrogen sulphur phosphorus of microbial preparation of utilizing.
Summary of the invention
In order to overcome the deficiencies in the prior art, effectively remove simply the pollutents such as ammonia nitrogen sulphur phosphorus in sewage, the invention provides a kind of method of utilizing microbial preparation to dispose of sewage, its technical scheme realizes by the following method:
A kind of method of utilizing microbial preparation to dispose of sewage, it comprises the steps:
The preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, standing 6-8 hour then, the most rearmounted 4 ℃ of lower cryodryings, dry after water content be controlled at 6%, obtain; By bamboo charcoal and chitosan, the mass ratio according to 1: 1 is mixed to get described carrier, and the particle diameter of bamboo charcoal is 10 orders preferably;
Sewage pre-treatment: at first, by sewage process solid-liquid separator, carry out solid-liquid separation, remove the bulk solid particulate matter, liquid enters settling tank subsequently, precipitates 12 hours, then liquid is removed to the solid throw out by the circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm
Microbiological oxidation: the liquid through the circular hole filtering net enters the microbial reaction pond, and regulating pH is 7, by every cubic metre of liquid, adds microbial preparation 10 grams at every turn, adds every day 1 time, adds continuously one week, last standing 3 days, liquid is discharged.
Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part:
10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps; The concentration of above-mentioned each raw material bacterium all is controlled at (1-2) * 10
8individual/ml.Above-mentioned bacterial classification can be the conventional bacterial strain of prior art,
Preferably,
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259 (reference APPLIED AND ENVIRONMENTAL MICROBIOLOGY for example, May 2007, p.3265-327 1);
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107 (for example CN101705202A);
Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589 (for example CN102168054A);
Described bacillus pumilus be bacillus pumilus (Bacilhs pumilus) ATCC 27142 (International Journal of Pharmaceutics for example, Volume 160, Issue 1,1998, Pages75-81);
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725 (for example APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714);
Described Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282 (for example CN102433277A).
Bacterium of the present invention all can be bought and obtain from commercial sources such as CGMCC, CCTCC and US mode culture collection warehousings (ATCC).
The enlarged culturing of each bacterial classification of the present invention is the cellar culture mode of this area, and the training method of putting down in writing in also can reference literature obtains.
The main beneficial effect that the present invention obtains is as follows: the present invention adopts pure microorganism treating sewage, environment friendly and pollution-free, with low cost, reasonable compatibility between each bacterial classification in microbiobacterial agent, symbiosis is coordinated, antagonism not mutually, and its preparation method is easy, easy to implement the method, it is easy and simple to handle, is beneficial to suitability for industrialized production; Process operations method of the present invention is simple, has reduced the wasting of resources, and less input, has reduced the cost of disposing of sewage; Treatment process of the present invention has purified sewage effectively, and dephosphorization, denitrogenation remove simultaneously, has solved the contradiction that in the traditional technology, dephosphorization and denitrification effect are difficult to take into account simultaneously.
Embodiment
The present invention is further elaborated below will to adopt the mode of specific embodiment, but it should not be construed the restriction to core initiative spirit of the present invention.
Embodiment 1
A kind of method of utilizing microbial preparation to dispose of sewage comprises:
The preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, then standing 6 hours, the most rearmounted 4 ℃ of lower cryodryings, dry rear water content is controlled at 6%, obtains; By particle diameter, to be 10 purpose bamboo charcoals and chitosan be mixed to get according to the mass ratio of 1: 1 described carrier; Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part: 10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps; The concentration of above-mentioned each raw material bacterium all is controlled at 1 * 10
8individual/ml.
The sewage pre-treatment: at first by sewage, (NH3-N is 198mg/L, sulfide 38 mg/L, P is 26 mg/L) through solid-liquid separator, carry out solid-liquid separation, remove the bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, again liquid is removed to the solid throw out by the circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm
Microbiological oxidation: the liquid through the circular hole filtering net enters the microbial reaction pond, and regulating pH is 7, by every cubic metre of liquid, adds microbial preparation 10 grams at every turn, adds every day 1 time, adds continuously one week, last standing 3 days, liquid is discharged.After testing, the clearance of ammonia nitrogen in sewage, sulphur and phosphor contaminant can reach respectively 98%, 98% and 99%.
Embodiment 2
A kind of method of utilizing microbial preparation to dispose of sewage comprises:
The preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, then standing 8 hours, the most rearmounted 4 ℃ of lower cryodryings, dry rear water content is controlled at 6%, obtains; By particle diameter, to be 10 purpose bamboo charcoals and chitosan be mixed to get according to the mass ratio of 1: 1 described carrier; Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight part: 10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps; The concentration of above-mentioned each raw material bacterium all is controlled at 2 * 10
8individual/ml; Above-mentioned thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259; Above-mentioned Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107; Above-mentioned sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589; Above-mentioned bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC 27142; Above-mentioned Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725; Above-mentioned Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282;
Sewage pre-treatment: at first by sewage (the sewage sample is from industry park, development area river wastewater) process solid-liquid separator, carry out solid-liquid separation, remove the bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, again liquid is removed to the solid throw out by the circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm
Microbiological oxidation: the liquid through the circular hole filtering net enters micronic dust thing reaction tank, and regulating pH is 7, by every cubic metre of liquid, adds microbial preparation 10 grams at every turn, adds every day 1 time, adds continuously one week, last standing 3 days, liquid is discharged.After testing, before and after the sewage of processing, pollutant load compares, in Table 1:
Table 1
Although, above with general explanation and embodiment, this case having been done to detailed explanation, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the modification done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (3)
1. a method of utilizing microbial preparation to dispose of sewage, is characterized in that, described method comprises the steps:
1) preparation of microbial preparation: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1: 1, stir, standing 6-8 hour then, the most rearmounted 4 ℃ of lower cryodryings, dry after water content be controlled at 6%, obtain; Described mixed bacteria liquid is mixed by the raw material bacterium of following weight part: 10 parts of thiobacillus denitrificans, 8 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, 5 parts of bacillus pumilus, 3 parts of Phanerochaete chrysosporiums, 2 parts of Chryseobacterium sps;
2) sewage pre-treatment: at first, by sewage process solid-liquid separator, carry out solid-liquid separation, remove the bulk solid particulate matter, liquid enters settling tank subsequently, precipitate 12 hours, then liquid is removed to the solid throw out by the circular hole filtering net, the Circularhole diameter of circular hole filtering net is 0.1mm;
3) microbiological oxidation: the liquid after the circular hole filtering net filters enters the microbial reaction pond, and regulating pH is 7, by every cubic metre of liquid, adds step 1 at every turn) microbial preparation 10 grams that prepare, add every day 1 time, add continuously one week, last standing 3 days, liquid is discharged.
2. the method for claim 1, is characterized in that, by bamboo charcoal and chitosan, the mass ratio according to 1: 1 is mixed to get described carrier.
3. method as claimed in claim 1 or 2, is characterized in that, described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacil1us denitrificans) ATCC25259; Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107; Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589; Described bacillus pumilus is bacillus pumilus (Bacillus pumilus) ATCC27142; Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725; Described Chryseobacterium sp is Chryseobacterium sp (Chryseobacterium sp.) CGMCC NO.5282.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104402174A (en) * | 2014-11-28 | 2015-03-11 | 云南圣清环保科技有限公司 | Deep treatment method of coal chemical industry wastewater |
| CN105036471A (en) * | 2015-07-26 | 2015-11-11 | 李娜 | Method for removing sewage pollutants containing lead |
| CN105060620A (en) * | 2015-07-26 | 2015-11-18 | 李娜 | Method for removing pollutants in arsenic-containing sewage |
| CN105174654A (en) * | 2015-10-16 | 2015-12-23 | 中国科学院水生生物研究所 | Deodorization method for landscape water body |
| CN105331598A (en) * | 2015-07-16 | 2016-02-17 | 国网山东省电力公司临沂供电公司 | Biological agent for treating ash sluicing water of cable channel |
| CN105907673A (en) * | 2016-04-29 | 2016-08-31 | 广州市佳境水处理技术工程有限公司 | Method for strengthening biochemical water treatment effects by microbial preparation |
| CN106045228A (en) * | 2016-08-03 | 2016-10-26 | 吴小慧 | Purification method of high-phosphorus-content wastewater |
| CN106862260A (en) * | 2017-03-10 | 2017-06-20 | 刘晨 | A kind of biochemical process for administering Cadmium Pollution in Soils |
| CN110002672A (en) * | 2019-04-07 | 2019-07-12 | 吉林化工学院 | A method of recycled water of the processing containing Chryseobacterium meningosepticum |
| CN112961124A (en) * | 2021-02-19 | 2021-06-15 | 杭州楠大环保科技有限公司 | Method for treating sewage by using microbial preparation |
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Cited By (14)
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| CN104402174A (en) * | 2014-11-28 | 2015-03-11 | 云南圣清环保科技有限公司 | Deep treatment method of coal chemical industry wastewater |
| CN105331598B (en) * | 2015-07-16 | 2018-05-22 | 裴桂良 | A kind of biological agent for being used to handle cable duct ash flushing water |
| CN105331598A (en) * | 2015-07-16 | 2016-02-17 | 国网山东省电力公司临沂供电公司 | Biological agent for treating ash sluicing water of cable channel |
| CN105036471A (en) * | 2015-07-26 | 2015-11-11 | 李娜 | Method for removing sewage pollutants containing lead |
| CN105060620A (en) * | 2015-07-26 | 2015-11-18 | 李娜 | Method for removing pollutants in arsenic-containing sewage |
| CN105174654A (en) * | 2015-10-16 | 2015-12-23 | 中国科学院水生生物研究所 | Deodorization method for landscape water body |
| CN105174654B (en) * | 2015-10-16 | 2018-08-21 | 中国科学院水生生物研究所 | A method of being used for landscape water body deodorization |
| CN105907673B (en) * | 2016-04-29 | 2018-02-06 | 广州市佳境水处理技术工程有限公司 | A kind of method using microorganism formulation enhanced water treatment biochemical effects |
| CN105907673A (en) * | 2016-04-29 | 2016-08-31 | 广州市佳境水处理技术工程有限公司 | Method for strengthening biochemical water treatment effects by microbial preparation |
| CN106045228A (en) * | 2016-08-03 | 2016-10-26 | 吴小慧 | Purification method of high-phosphorus-content wastewater |
| CN106862260A (en) * | 2017-03-10 | 2017-06-20 | 刘晨 | A kind of biochemical process for administering Cadmium Pollution in Soils |
| CN110002672A (en) * | 2019-04-07 | 2019-07-12 | 吉林化工学院 | A method of recycled water of the processing containing Chryseobacterium meningosepticum |
| CN112961124A (en) * | 2021-02-19 | 2021-06-15 | 杭州楠大环保科技有限公司 | Method for treating sewage by using microbial preparation |
| CN112961124B (en) * | 2021-02-19 | 2022-06-21 | 杭州楠大环保科技有限公司 | Method for treating sewage by using microbial preparation |
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Address after: 510010 Room A510, Fifth Floor, Block A, Hongyun Building, 37 Station West Road, Yuexiu District, Guangzhou City, Guangdong Province Patentee after: Guangzhou Jianisi Anti-mildew and Antibacterial Technology Co., Ltd. Address before: 510010 Room A510, Fifth Floor, Block A, Hongyun Building, 37 Station West Road, Yuexiu District, Guangzhou City, Guangdong Province Patentee before: GOOD NICE ANTI-MOLD CO., LTD. |
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