CN114751520B - Method for treating ammonia sugar processing wastewater by utilizing fungus microalgae symbiotic system - Google Patents
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Abstract
The invention discloses a method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system, belonging to the technical field of biological environment environmental protection. According to the invention, the fungus microalgae symbiotic system is constructed, and euglena gracilis, melanomyces nigricans and mucor gracilis are domesticated to form the phycomycete microspheres, so that the sewage treatment effect is ensured, the phycomycete groups can be naturally settled, the treatment capacity of centrifugal recovery is reduced, and the problem of pipeline blockage caused by bacterial group suspension is avoided. The algicidal symbiotic system is used for directly treating the ammonia sugar processing wastewater to replace the prior processes of chemical treatment and regenerant treatment, so that the problem of secondary pollution caused in the use process of chemical reagents is avoided. The ammonia sugar processing wastewater obtained by the treatment of the method of the invention meets the emission standard of extracted pharmaceutical industry water pollutants, and has important promotion significance for promoting ammonia sugar processing production.
Description
Technical Field
The invention relates to a method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system, belonging to the technical field of biological environment protection.
Background
Amino sugar (GlcN), known as glucosamine, formula C 6 H 13 NO 5 The polysaccharide is formed by replacing one hydroxyl group of glucose by an amino group, and is usually present in shrimp and crab shells and fungal cell walls in the form of polysaccharide or conjugated polysaccharide in nature. This organic substance is a substance essential for proteoglycan synthesis in the matrix of human articular cartilage, and it is used as a basic substanceChondroitin agents are widely used for the prevention and treatment of osteoarticular diseases.
However, the GlcN industry is under tremendous environmental pressure. The raw material library for GlcN preparation is mainly shrimp shells, and the main preparation process comprises the following steps: removing calcium carbonate, deproteinizing and decoloring shrimp shells to obtain chitin; carrying out acidolysis on chitin at a high temperature to obtain a GlcN crude product; and filtering and deslagging the GlcN crude product, and recrystallizing to obtain a purified product. Wherein, the shrimp shell calcium carbonate removal needs to be soaked by hydrochloric acid with the concentration of 5 percent; the acidolysis of chitin requires the use of hydrochloric acid with a concentration of 30%, and about 8.5 tons of hydrochloric acid with a concentration of 30% is consumed for each 1 ton of GlcN produced, thereby resulting in high COD value and high chloride content (Cl) of GlcN processing wastewater - Concentration 3% -5%). Chlorine radicals in GlcN wastewater are mostly in inorganic form (Cl) - ) The chlorine salt is formed together with metal ions in the water body, while the light metal chlorine salt generally has good water solubility and is not easy to be removed from the water body, if the treatment is not thorough, the waste water discharged into the water body can destroy fishery production, aquaculture and fresh water resources, seriously can pollute underground water and drinking water sources, and seriously can lead to Cl under certain specific conditions - Can produce chemical action with organic matters in water to generate a series of chlorohydrocarbons, and has a certain carcinogenic effect on human bodies. At present, wastewater treatment becomes a problem to be solved urgently in the industrial upgrading process of GlcN processing enterprises.
At present, the dilution method is commonly used for reducing Cl in wastewater - Concentration of (2), i.e. raw wastewater with low Cl - Mixing the loaded waste water, air-floating, oxidizing and precipitating to reduce COD (chemical oxygen demand) of water to about 0.8g/L, further diluting to make the water reach three-stage discharge standard (COD)<0.5 g/L). The dilution method has the advantages of low operation cost and simple and convenient operation, but the requirements of sites and water quantity are large, the total COD (chemical oxygen demand) is not reduced, and the method has no long-term property. Cl for other applications - The removing method comprises the following steps: (1) Precipitation method, use of pharmaceutical agents and Cl - The precipitate is removed by centrifugation or filtration. Since light metal chloride salts are readily soluble in water, heavy metal-containing agents such as silver nitrate and polymeric ferrous sulfate are required. (2) The ion-exchange method is adopted, and the ion exchange method is adopted,adding Cl - Adsorbing onto polymer material with positive charge, and adding OH - Or other anions with Cl - Instead, cl was collected - . (3) Membrane separation, which is divided into two ways of electrodialysis and reverse osmosis, i.e. Cl driven by an external direct current electric field or pressure - The permeation membrane material moves to one side to achieve the enrichment effect and then is removed. The treatment technologies have the characteristics of high cost or secondary pollution, and limit the application in practical engineering.
The removal of chloride ions is mainly to realize the subsequent biological treatment of wastewater and reach the standard of recycled water chloride, and the biological treatment can simultaneously remove Cl - COD and BOD (biological oxygen demand). The biological treatment purifies the waste water by microorganisms, the microorganisms convert or degrade various substances in the waste water for the growth and reproduction of the microorganisms, or indirectly promote the flocculation and sedimentation of various ions, so that the waste water is purified, and the biological treatment comprises three processes, namely aerobic treatment, anaerobic treatment and anaerobic/aerobic combined process. Aerobic processes have great advantages in treating low salinity wastewater, but when Cl is present in the wastewater - When the concentration is more than 3g/L, the inhibition effect on an aerobic biological treatment system is generated, and the degradation capability of microorganisms is greatly reduced; under the anaerobic condition, the growth of microorganisms is slow, the biological activity is improved, and when anions and cations exist simultaneously, antagonism is generated, so that the salt concentration of the high-salinity wastewater can be effectively reduced, and people always screen and domesticate the salt-tolerant microorganisms capable of growing under the anaerobic condition.
The anaerobic or facultative anaerobic salt-tolerant microbes are fermented under the anaerobic condition, and can stably and effectively reduce Cl in the high-salinity wastewater - And in a certain COD concentration range, cl - The removal rate is in positive correlation with the COD value of the wastewater, the treatment temperature and the time. For example, mendez utilizes mesophilic anaerobic filters to treat high salt industrial wastewater, and increases Cl-clearance from 68% to 85% with increasing COD value, even though Cl-is present in wastewater with COD value in the range of 10-50g/L - The concentration is increased to 13 percent, the experiment still runs stably, the COD removal rate reaches 64 percent, and when the high-temperature anaerobic filter tank is adopted for treatment, the COD removal rate is increased to73 percent. Nobel et al used a downflow anaerobic mixed bed reactor to treat piggery wastewater (1.5% salt), the hydraulic retention time was extended from 12 hours to 96h and the COD removal rate was increased from 68% to 90%. However, the most common method in industry is to remove Cl by a physicochemical method - In the biological treatment of dechlorination waste water, the reason is mainly that zoogloea flocs are loose and are in a suspension state in a water body, the sewage treatment capacity and treatment load are not high, and pipelines are often blocked, so that the normal operation of a system is influenced.
When filamentous fungi and microalgae are co-grown under a proper condition, the secretion of the fungi can promote the growth of the microalgae, and the microalgae is used as a core to promote hyphae of the fungi to form clustered microspheres, so that the sedimentation effect is good, and the phycomycete symbiotic system has wide application in the field of removing water eutrophication, for example, chlorella (Chlorella vulgaris) and Mucor circinelloides (Mucor circinelloides) are co-cultured, the COD removal rate of the water can reach 86% at most, the gravity sedimentation rate of the microorganisms reaches 99%, and the treatment amount of centrifugal recovery is effectively reduced. The symbiotic growth of the mold (Aspergillus niger) and the chlorella, the biomass yield in the water body is also improved by 67 percent, and after the exponential growth phase is finished, the removal rate of ammonia in the water body reaches 30 percent (v/v) and the removal rate of phosphorus reaches 62 percent (v/v). However, the symbiotic relationship between microalgae and fungi is selective, only suitable strains can jointly grow and act on sewage treatment together, unsuitable fungi have an algae dissolving effect, and algal cells can decline along with the prolonging of the co-culture time. Therefore, if a fungal microalgae symbiotic system suitable for treating ammonia sugar processing wastewater can be constructed, the fungal microalgae symbiotic system has important promotion significance for promoting GlcN processing production.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system, which is used for carrying out biological treatment on the ammonia sugar processing wastewater, particularly treating inorganic chloride ions in the ammonia sugar processing wastewater, and adding a green and environment-friendly treatment method on the basis of adopting a physical or chemical method for treatment to solve the problem of secondary pollution; in addition, the invention uses two filamentous fungi and one microalgae to form a symbiotic system to treat the wastewater, thereby solving the problem that the zoogloea suspension hinders the normal operation of sewage treatment facilities.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system, which comprises the following steps:
(1) Pre-culturing microalgae: euglena gracilis var. Saccharophila is cultured in Hunter's culture solution three months before use, and then cultured in ammonia sugar processing wastewater to obtain algae cells;
(2) Pre-culturing fungi: inoculating Aspergillus niger and Mucor hiemalis in G-YPD culture medium for the first three months, inoculating into new G-YPD culture medium, performing multiple transfer culture, and collecting two fungal spores with sterilized 0.05% Tween solution;
(3) Constructing an algae and bacteria symbiotic system: adding the two fungal spores obtained in the step (2) into the ammonia sugar processing wastewater, adding the algal cells obtained in the step (1) after outdoor culture, simultaneously starting aeration, carrying out co-culture, stopping aeration when the spherical microspheres with the diameter of 6-10 mm are visible by naked eyes in a reaction tank, settling the algal bacteria microspheres, discharging supernatant, continuously injecting new ammonia sugar processing wastewater, continuously culturing, and carrying out 3-4 cycles to obtain an algal bacteria symbiotic system; the algae-bacteria symbiotic system is easy to construct, stable in operation and capable of being switched repeatedly, concentrated algae seeds and strains can be stored at low temperature when the system is idle, and investment required by periodic maintenance is avoided.
(4) Pretreatment of ammonia sugar processing wastewater: treating the ammonia sugar processing wastewater by a grid, a screen and a grit chamber, adjusting the pH value to 4-6, and then pre-aerating for 3-5 h to obtain the pretreated ammonia sugar processing wastewater;
(5) Biological treatment of ammonia sugar processing wastewater: and (3) injecting the pretreated ammonia sugar processing wastewater obtained in the step (4) into a reaction tank containing the phycomycete symbiotic system prepared in the step (3), aerating, stopping aerating, draining the ammonia sugar processing wastewater to a sedimentation tank through a filter screen (phi 5 mm), draining the supernatant to a secondary sedimentation tank through a filter screen (phi 2 mm), adding an algicide agent into the secondary sedimentation tank, uniformly stirring, and taking an overflow liquid to realize the treatment of the ammonia sugar processing wastewater.
Further, in the step (1), 200ml of Hunter's culture solution was added to the culture solution so that the total number of Euglena lanuginosa cells was 1X 10 9 ～1.7×10 9 The culture condition in Hunter culture solution is normal temperature illumination shake culture, and the culture time is 3-4 days.
Further, the specific steps of transferring the glucosamine into the wastewater from the glucosamine processing step (1) for culturing are as follows: culturing in Hunter culture solution for 3-4 days, transferring to 100ml of ammonia sugar processing wastewater, then adding 100ml of ammonia sugar processing wastewater into every two circumferential culture systems, when the volume of the culture solution reaches 600ml, transferring all the culture solution into a 10L large-opening glass jar container, adding ammonia sugar processing wastewater to the scale, sealing the outdoor, and culturing for 3 days in an aerated manner to obtain the algae cells.
Further, the step (2) is specifically as follows: the black mold (Aspergillus niger) and the Mucor hiemalis are inoculated in a G-YPD culture medium and cultured for 7 to 9 days at normal temperature, then the culture medium is transferred to a new G-YPD culture medium, the nutrient components of the original YPD culture medium are reduced by 12 to 20 percent once the culture medium is transferred until the final plate culture medium only contains two components of ammonia sugar processing wastewater and agar, and finally, the two fungal spores are collected by using a sterilized 0.05 percent Tween solution.
Further, the G-YPD medium contains 1% (w/v) of yeast extract, 2% (w/v) of peptone, 2% (w/v) of glucose and 2% (w/v) of agar powder, and is prepared by mixing and suspending the components in ammonia sugar processing wastewater and sterilizing.
Further, the amount of the black mold added to the ammonia sugar processing wastewater in the step (3) is (5.0X 10) 10 ～6.0×10 10 ) The addition amount of mucor is about (1.0X 10) per cubic meter 12 ～1.6×10 12 ) Per cubic meter, the dosage of algae cell is (1 × 10) 12 ～1.5×10 12 ) One per cubic meter.
Further, adding two kinds of fungal spores in the step (3) for outdoor culture for 4-6 days, wherein aeration is performed at intervals of 10-12 hours every day, and the number of days of co-culture is 10-14 days.
Further, in the step (5), the phycomycete symbiotic system is added into the pretreated ammonia sugar processing wastewater to ensure that the concentration of the phycomycete microspheres is 5-6.2 g/L.
Further, in the step (5), the retention time of each batch of pretreated ammonia sugar processing wastewater in the reaction tank is 8-10 h, the retention time in the sedimentation tank is 5-8 h, and the retention time in the secondary sedimentation tank is 12-16 h.
Further, the concentration of the algicide agent potassium monopersulfate in the step (5) is 0.01 to 0.06ppm.
Further, suspended matters and Cl in the overflow liquid in the step (5) - 、COD Cr 、BOD 5 And the indexes such as pH value and the like all accord with the emission standard of extracted pharmaceutical industrial water pollutants (GB 21905-2008).
The invention discloses the following technical effects:
(1) According to the invention, the microalgae microsphere is formed by constructing the microalgae-fungi symbiotic system, so that the sewage treatment effect is ensured, the algal cenospheres can be naturally settled, the treatment capacity of centrifugal recovery is reduced, and the problem of pipeline blockage caused by cenospheres suspension is avoided.
(2) The invention applies the algae bacterium symbiotic system to directly treat the ammonia sugar processing wastewater, replaces the prior processes of chemical treatment and regeneration treatment, and avoids the problem of secondary pollution caused in the use process of chemical reagents.
(3) The ammonia sugar processing wastewater obtained by the treatment of the method meets the emission standard of extracted pharmaceutical industrial water pollutants (GB 21905-2008).
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every intervening value, to the extent any stated value or intervening value in a stated range, and any other stated or intervening value in a stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The euglena gracilis used in the embodiment of the invention is purchased from an algae seed bank (with the number of UTEX 752) of the university of Texas in the United states, the melanomyces and the trichoderma are obtained by separating and purifying from the mangrove wetland by the inventor, and the methods for separating and purifying are all conventional technical means in the field and do not serve as the invention points, so that redundant description is not repeated.
The G-YPD culture medium, the Hunter culture solution, the Tween solution and the algicide agent potassium monopersulfate which are used for implementing the invention are all related purchased components and prepared by the inventor, and the components of the solutions are all known to the skilled person and are not described in detail.
The wastewater from the glucosamine processing in the embodiment of the invention is obtained from Fujian Huakang pharmaceutical industry Co.
The specific preparation steps of the G-YPD culture medium used in the embodiment of the invention are as follows: taking preparation of 1L of culture medium as an example, 10g of yeast extract, 20g of peptone and 20g of agar powder are suspended in 900ml of ammonia sugar processing wastewater, and sterilized at 121 ℃ under high pressure for 15min. Another 20g of glucose was dissolved in 60mL of ammonia sugar processing wastewater and sterilized by filtration through a 0.22 μm filter. Mixing the two sterilized solutions, and adding sterilized ammonia sugar processing wastewater to 1L.
The technical solution of the present invention is further illustrated by the following examples.
Example 1
A method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system comprises the following steps:
(1) Pre-culturing microalgae: euglena gracilis var. Saccharophila was cultured in a 2000ml shake flask with 200ml Hunter broth three months before use, so that the total number of Euglena gracilis cells was 1X 10 9 Performing shake culture for 3 days under normal temperature illumination, then transferring into 100ml of glucosamine processing wastewater, then adding 100ml of glucosamine processing wastewater into every two circumferential culture systems, when the volume of a culture solution reaches 600ml, transferring all the culture solution into a 10L large-opening glass jar container, adding the glucosamine processing wastewater to a scale, and sealing and ventilating outdoors for 3 days to obtain algal cells;
(2) Pre-culturing fungi: inoculating black mold (Aspergillus niger) and Mucor hiemalis (Mucor hiemalis) into G-YPD culture medium for three months, culturing at normal temperature for 8 days, transferring into new G-YPD culture medium, reducing nutrient components of original YPD culture medium by 20% every time of transferring until the final plate culture medium only contains two components of ammonia sugar processing wastewater and agar, and collecting two fungal spores by using sterilized 0.05% Tween solution;
(3) Constructing an algae and bacteria symbiotic system: the ammonia sugar processing wastewater after deslagging and primary sedimentation is injected into an open reaction tank, the two fungal spores obtained in the step (2) are added into the ammonia sugar processing wastewater, and the adding amount of the melanomyces is 5.0 multiplied by 10 10 The addition amount of mucor is about 1.0 × 10 per cubic meter 12 Culturing for 5 days in outdoor environment, adding the algae cells obtained in the step (1), wherein the adding amount of the algae cells is 1.5 multiplied by 10 12 Per cubic meter while aeration is started every dayAerating for 12h at intervals, co-culturing for 12 days, stopping aeration when the spherical microspheres with the diameter of 9mm are visible in the reaction tank by naked eyes, settling the phycomycete microspheres, discharging supernatant, continuously injecting new ammonia sugar processing wastewater, continuously culturing and carrying out 4 cycles to obtain the phycomycete symbiotic system;
(4) Pretreatment of ammonia sugar processing wastewater: treating the ammonia sugar processing wastewater by a grid, a screen and a grit chamber, adjusting the pH value to 4, and then pre-aerating for 3 hours to obtain pretreated ammonia sugar processing wastewater;
(5) Biological treatment of ammonia sugar processing wastewater: injecting the pretreated ammonia sugar processing wastewater obtained in the step (4) into a reaction tank, adding the algae bacteria symbiotic system prepared in the step (3), enabling the concentration of algae bacteria microspheres to be 5g/L after the algae bacteria symbiotic system, aerating, enabling the pretreated ammonia sugar processing wastewater to stay in the reaction tank for 9h, then stopping aeration, draining the ammonia sugar processing wastewater into a conical sedimentation tank through a filter screen (phi 5 mm) within 4h, staying for 6h, draining supernatant into a secondary sedimentation tank through a filter screen (phi 2 mm), adding algicidal agent potassium monopersulfate into the secondary sedimentation tank, uniformly stirring, staying for 15h, and taking overflow liquid to realize the treatment of the ammonia sugar processing wastewater.
Example 2
A method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system comprises the following steps:
(1) Pre-culturing microalgae: the same as example 1;
(2) Pre-culturing fungi: inoculating black mold (Aspergillus niger) and Mucor hiemalis (Mucor hiemalis) into G-YPD culture medium for three months, culturing at normal temperature for 9 days, transferring into new G-YPD culture medium, reducing nutrient components of original YPD culture medium by 20% every time of transferring until the final plate culture medium only contains two components of ammonia sugar processing wastewater and agar, and collecting two fungal spores by using sterilized 0.05% Tween solution;
(3) Constructing an algae and bacteria symbiotic system: the ammonia sugar processing wastewater after deslagging and primary sedimentation is injected into an open reaction tank, the two fungal spores obtained in the step (2) are added into the ammonia sugar processing wastewater, and the adding amount of the melanomyces is 5.5 multiplied by 10 10 Standing/standingThe addition amount of the square rice and the mucor is about 1.3 multiplied by 10 12 Culturing for 4 days in the open air per cubic meter, and adding the algae cells obtained in the step (1) in an amount of 1.25 × 10 12 Aerating per cubic meter simultaneously, aerating for 12h every day at intervals, culturing for 14 days together, stopping aeration when the spherical microspheres with the diameter of 6mm are visible by naked eyes in the reaction tank, settling the phycomycetes microspheres, discharging supernatant, continuously injecting new glucosamine processing wastewater, continuously culturing and carrying out 3 cycles to obtain the phycomycetes symbiotic system;
(4) Pretreatment of ammonia sugar processing wastewater: treating the ammonia sugar processing wastewater by a grid, a screen and a grit chamber, adjusting the pH value to 5, and then pre-aerating for 4 hours to obtain pretreated ammonia sugar processing wastewater;
(5) Biological treatment of ammonia sugar processing wastewater: injecting the pretreated ammonia sugar processing wastewater obtained in the step (4) into a reaction tank, adding the phycobiont system prepared in the step (3) to ensure that the concentration of phycomycete microspheres is 5.6g/L after the phycomycete symbiont system, aerating to ensure that the pretreated ammonia sugar processing wastewater stays in the reaction tank for 8h, then stopping aerating, draining the ammonia sugar processing wastewater into a conical sedimentation tank through a filter screen (phi 5 mm) within 3h, staying for 5h, then draining supernatant into a secondary sedimentation tank through a filter screen (phi 2 mm), adding algicidal potassium monopersulfate into the secondary sedimentation tank, uniformly stirring, staying for 16h, and taking overflow liquid to realize the treatment of the ammonia sugar processing wastewater.
Example 3
A method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system comprises the following steps:
(1) Pre-culturing microalgae: the same as example 1;
(2) Pre-culturing fungi: inoculating black mold (Aspergillus niger) and Mucor hiemalis (Mucor hiemalis) into G-YPD culture medium for three months, culturing at normal temperature for 7 days, transferring into new G-YPD culture medium, reducing nutrient components of original YPD culture medium by 20% every time of transferring until the final plate culture medium only contains two components of ammonia sugar processing wastewater and agar, and collecting two fungal spores by using sterilized 0.05% Tween solution;
(3) Constructing an algae and bacteria symbiotic system: the ammonia sugar processing wastewater after deslagging and primary sedimentation is injected into an open reaction tank, the two fungal spores obtained in the step (2) are added into the ammonia sugar processing wastewater, and the adding amount of the melanomyces is 6.0 multiplied by 10 10 The addition amount of mucor is about 1.6 x 10 per cubic meter 12 Culturing for 6 days in outdoor environment, adding the algae cells obtained in the step (1), wherein the adding amount of the algae cells is 1.5 multiplied by 10 12 Aerating per cubic meter simultaneously, aerating for 12 hours every day at intervals, culturing for 10 days together, stopping aeration when the spherical microspheres with the diameter of 10mm are visible by naked eyes in the reaction tank, settling the phycomycete microspheres, discharging supernatant, continuously injecting new ammonia sugar processing wastewater, continuously culturing and carrying out 4 cycles to obtain the phycomycete symbiotic system;
(4) Pretreatment of ammonia sugar processing wastewater: treating the ammonia sugar processing wastewater by a grid, a screen and a grit chamber, adjusting the pH value to 5.2, and then pre-aerating for 3h to obtain pretreated ammonia sugar processing wastewater;
(5) Biological treatment of ammonia sugar processing wastewater: injecting the pretreated ammonia sugar processing wastewater obtained in the step (4) into a reaction tank, adding the algae bacteria symbiotic system prepared in the step (3), enabling the concentration of algae bacteria microspheres to be 6.2g/L after the algae bacteria symbiotic system, aerating, enabling the pretreated ammonia sugar processing wastewater to stay in the reaction tank for 10h, then stopping aeration, draining the ammonia sugar processing wastewater into a conical sedimentation tank through a filter screen (phi 5 mm) within 2h, staying for 8h, draining supernatant into a secondary sedimentation tank through the filter screen (phi 2 mm), adding an algicidal agent potassium monopersulfate into the secondary sedimentation tank, uniformly stirring, staying for 12h, and taking an overflow liquid to realize the treatment of the ammonia sugar processing wastewater.
Results of treatment of glucosamine processing wastewater
The overflow liquid obtained by the treatment of the examples 1 to 3 and the untreated ammonia sugar processing wastewater are subjected to suspension and Cl - 、COD Cr 、BOD 5 And comparing pH indexes, wherein the standard for detecting each index is as follows: the suspension is according to GB/T11901-1989; cl - According to GB/T11896-1989; COD Cr According to GB/T11901-1989; BOD 5 According to GB/T7488-1987;the pH was according to GB/T6920-1986, the results are shown in Table 1.
TABLE 1 test results (unit: mg/L)
As is clear from the contents of Table 1, the ammonia sugar processing wastewater treated by the method of example 1, 2 or 3 contains suspended matter and Cl - 、COD Cr 、BOD 5 The pH index accords with the discharge standard of the extracted pharmaceutical industrial water pollutants (GB 21905-2008), and in the wastewater treatment process, the problem of pipeline blockage caused by the suspension of the bacterial colony does not occur, the trehalose symbiotic system is applied to directly treat the ammonia sugar processing wastewater, the existing processes of chemical treatment and regenerant treatment are replaced, and the problem of secondary pollution caused in the use process of chemical reagents is avoided.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (5)
1. A method for treating ammonia sugar processing wastewater by utilizing a fungus microalgae symbiotic system is characterized by comprising the following steps:
(1) Pre-culturing microalgae: culturing euglena gracilis in Hunter culture solution, and culturing in ammonia sugar processing wastewater to obtain algae cells;
(2) Pre-culturing fungi: inoculating black mold and trichoderma into a yeast leaching peptone glucose culture medium, namely a G-YPD culture medium prepared from ammonia sugar processing wastewater, then transferring the culture medium into a new G-YPD culture medium, performing transfer culture for multiple times, and finally collecting two fungal spores by using a sterilized 0.05% Tween solution;
(3) Constructing an algae-bacteria symbiotic system: adding two fungal spores obtained in the step (2) into the ammonia sugar processing wastewater, adding the algal cells obtained in the step (1) after outdoor culture, simultaneously starting aeration, carrying out co-culture, then stopping aeration to enable the algal bacteria microspheres to settle, discharging supernatant, continuously injecting new ammonia sugar processing wastewater, continuously culturing and circulating to obtain an algal bacteria symbiotic system;
(4) Pretreatment of ammonia sugar processing wastewater: treating the ammonia sugar processing wastewater by a grid, a screen and a grit chamber, adjusting the pH value, and then pre-aerating to obtain pretreated ammonia sugar processing wastewater;
(5) Biological treatment of ammonia sugar processing wastewater: injecting the pretreated ammonia sugar processing wastewater obtained in the step (4) into a reaction tank containing the phycomycete symbiotic system prepared in the step (3), aerating, stopping aerating, draining the ammonia sugar processing wastewater to a sedimentation tank through a filter screen, draining supernatant to a secondary sedimentation tank through the filter screen, adding an algicide agent into the secondary sedimentation tank, stirring uniformly, and taking overflow liquid to realize the treatment of the ammonia sugar processing wastewater;
the addition amount of the black mold added into the ammonia sugar processing wastewater in the step (3) is (5.0 multiplied by 10) 10 ~6.0×10 10 ) The addition amount of mucor is (1.0X 10) per cubic meter 12 ~1.6×10 12 ) Per cubic meter, the dosage of algae cell is (1 × 10) 12 ~1.5×10 12 ) Per cubic meter;
adding two fungal spores into the mixture in the step (3) for outdoor culture for 4~6 days, wherein aeration is carried out for 10 to 12h every day at intervals, and the number of days of co-culture is 10 to 14 days;
and (5) adding an algal-bacteria symbiotic system into the pretreated ammonia sugar processing wastewater to ensure that the concentration of the algal-bacteria microspheres is 5 to 6.2g/L.
2. The method for treating wastewater from ammonia sugar process according to claim 1, wherein the total amount of Euglena tenuis cells added to 200ml of Hunter's culture solution in step (1) is 1X 10 9 ~1.7×10 9 Culturing in Hunter culture solution under normal temperature illumination shake culture for 3-4 days.
3. The method for treating wastewater from glucosamine processing according to claim 1, wherein the step of transferring to wastewater from glucosamine processing in step (1) comprises the steps of: culturing 3~4 days in Hunter culture solution, transferring 100ml of ammonia sugar processing wastewater, adding 100ml of ammonia sugar processing wastewater into every two circumferential culture systems, transferring all the culture solution into a 10L container when the volume of the culture solution reaches 600ml, adding ammonia sugar processing wastewater to the scale, sealing the outdoor, and culturing in an aerated manner to obtain the algae cells.
4. The method for treating wastewater from glucosamine processing according to claim 1, wherein the step (2) comprises: the method comprises the steps of inoculating black mold and mucor into a G-YPD culture medium, culturing at normal temperature for 7~9 days, transferring into a new G-YPD culture medium, reducing nutrient components in the original YPD culture medium by 12% -20% every time of transferring until the final plate culture medium only contains two components of ammonia sugar processing wastewater and agar, and finally collecting two fungal spores by using a sterilized 0.05% Tween solution.
5. The method for treating wastewater from glucosamine processing as defined in claim 1, wherein each batch of wastewater from step (5) is allowed to stay in the reaction tank for 8 to 10h, in the sedimentation tank for 5 to 8h, and in the secondary sedimentation tank for 12 to 169h.
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