CN114751520A - A method for treating wastewater from glucosamine processing by using fungal-microalgae symbiosis system - Google Patents

Abstract

The invention discloses a method for treating wastewater from ammonia sugar processing by utilizing a fungal-microalgae symbiotic system, belonging to the technical field of biological environment protection. By constructing a fungal-microalgae symbiosis system, the present invention domesticates Euglena slenderness, black mold, and mucor mold to form algal microspheres, which not only ensures the sewage treatment effect, but also enables the algal flora to settle naturally, and reduces the processing capacity of centrifugal recovery. At the same time, the problem of pipeline blockage caused by the suspension of bacterial mass is avoided. The algae-bacteria symbiosis system is used to directly treat ammonia sugar processing wastewater, replacing the current process of first chemical treatment and then biological treatment, so as to avoid the problem of secondary pollution caused by the use of chemical reagents. The glucosamine processing wastewater obtained by the method of the present invention complies with the water pollutant discharge standard of extraction pharmaceutical industry, and has important promoting significance for promoting the processing and production of glucosamine.

Description

A method for treating wastewater from glucosamine processing by using fungal-microalgae symbiosis system

technical field

The invention relates to a method for using a fungal-microalgae symbiotic system to process waste water from glucosamine processing, and belongs to the technical field of biological environment protection.

Background technique

Glucosamine (GlcN), full name of glucosamine, molecular formula C ₆ H ₁₃ NO ₅ , is formed by the substitution of a hydroxyl group of glucose by an amino group. It usually exists in the form of polysaccharide or conjugated polysaccharide in shrimp, crab shells and fungal cell walls in nature. This organic substance is necessary for the synthesis of proteoglycan in human articular cartilage matrix, and its chondroitin agent is widely used in the prevention and treatment of bone and joint diseases.

However, the development of GlcN industry faces enormous environmental pressure. The raw material library for GlcN preparation is mainly shrimp shells. The main preparation process includes: decalcification, deproteinization, and decolorization of shrimp shells to obtain chitin; chitin is acidly hydrolyzed at high temperature to obtain crude GlcN; the crude GlcN is filtered to remove residue and recrystallize Afterwards, the purified product was obtained. Among them, the decalcification of shrimp shells needs to be soaked in hydrochloric acid with a concentration of 5%; the acid hydrolysis of chitin needs to use hydrochloric acid with a concentration of 30%. For every 1 ton of GlcN prepared, about 8.5 tons of hydrochloric acid with a concentration of 30% needs to be consumed. GlcN processing wastewater has the characteristics of high COD value and high chloride content (Cl ^- concentration 3%-5%). Chloride in GlcN wastewater mostly exists in inorganic form (Cl ^- ), and forms chloride salts together with metal ions in the water body, while light metal chloride salts generally have good water solubility and are not easy to remove from the water body. After these waste water is discharged into the water body, it will destroy fishery production, aquaculture and fresh water resources, and in serious cases will also pollute groundwater and drinking water sources. In serious cases, under certain specific conditions, Cl ^- can produce chemical effects with organic matter in the water. , to generate a series of chlorinated hydrocarbons, which have a certain degree of carcinogenic effect on the human body. At present, wastewater treatment has become an urgent problem to be solved in the process of industrial upgrading of GlcN processing enterprises.

At present, people often use dilution method to reduce the concentration of Cl ^- in wastewater, that is, mixing the original wastewater with wastewater with low Cl ^- load, and after air flotation, oxidation and sedimentation treatment, the COD (chemical oxygen demand) of water quality is reduced to 0.8g/ About L, and then further diluted to make the water quality reach the third-level discharge standard (COD<0.5g/L). The dilution method has low operating cost and is easy to operate, but requires large space and water, and the total amount of COD does not decrease, so it is not long-term. Other Cl ^- removal methods with smaller application range include: (1) precipitation method, using chemicals and Cl ^- to generate precipitates, and removing the precipitates by centrifugation or filtration. Since light metal chloride salts are easily soluble in water, heavy metal-containing agents, such as silver nitrate and polyferrous sulfate, need to be used. (2) Ion exchange method, adsorb Cl ^- on the positively charged polymer material, and then replace Cl ^- with OH ^- or other anions, thereby collecting Cl ^- . (3) Membrane separation method, which is further divided into two ways: electrodialysis and reverse osmosis, that is, driven by an external DC electric field or pressure, the Cl ^- permeable membrane material moves to one side to achieve the effect of enrichment, and then remove. These treatment technologies generally have the characteristics of high cost or secondary pollution, which limit their application in practical engineering.

Removal of chloride ions is mainly to achieve subsequent biological treatment of wastewater and meet the chloride standard of reuse water, and biological treatment can simultaneously remove Cl ^- , COD and BOD (biological oxygen demand). Biological treatment purifies wastewater through microorganisms. Microorganisms convert or degrade various substances in wastewater for their own growth and reproduction, or indirectly promote the flocculation and sedimentation of various ions, so that wastewater can be purified and biologically treated. There are three types of aerobic treatment, anaerobic treatment and anaerobic/aerobic combined process. The aerobic method has great advantages in the treatment of low salinity wastewater, but when the Cl ^- concentration in the wastewater is greater than 3g/L, it will inhibit the aerobic biological treatment system, and the microbial degradation ability will be greatly reduced; Under oxygen conditions, the growth of microorganisms is slow, but the biological activity is improved. When anions and cations exist at the same time, an antagonistic effect can be produced, which can effectively reduce the salt concentration of high-salinity wastewater. Growth of salt-tolerant microorganisms.

The fermentation of anaerobic or facultative anaerobic salt-tolerant microorganisms under anaerobic conditions can steadily and effectively reduce the concentration of Cl ^- in high ^- salt wastewater. There is a positive correlation between processing temperature and time. For example, Mendez uses a mesophilic anaerobic filter to treat high-salt industrial wastewater. When the COD value of the wastewater is in the range of 10-50g/L, the Cl- removal rate increases from 68% to 85% with the increase of the COD value. Even if the Cl ^- concentration is increased to 13%, the experiment still runs smoothly, and the COD removal rate reaches 64%. When the high temperature anaerobic filter is used for treatment, the COD removal rate increases to 73%. Nobel et al. used a downflow anaerobic mixed bed reactor to treat pig farm wastewater (salt content of 1.5%), the hydraulic retention time was extended from 12h to 96h, and the COD removal rate was increased from 68% to 90%. However, the most commonly used method in the industry is to remove Cl ^- by the first physicochemical method. In the biological treatment of dechlorinated wastewater, the main reason is that the bacterial micelle flocs are loose and suspended in the water body, and the sewage treatment capacity and treatment load are not high. , and often cause pipeline blockage, affecting the normal operation of the system.

When filamentous fungi and microalgae grow together under suitable conditions, the secretion of fungi can promote the growth of microalgae, and with the microalgae as the core, the fungal hyphae can be formed into agglomerated microspheres, and the sedimentation effect is good. The bacterial symbiosis system has a wide range of applications in the removal of eutrophication in water bodies. For example, when Chlorella vulgaris and Mucor circinelloides are co-cultured, the water COD removal rate can reach up to 86%, and the gravitational sedimentation of microorganisms The rate reaches 99%, which effectively reduces the processing capacity of centrifugal recycling. The co-growth of mold (Aspergillus niger) and chlorella also increased the biomass production in the water body by 67%. After the exponential growth period, the removal rate of ammonia in the water body reached 30% (v/v) and the removal rate of phosphorus. 62(v/v). However, the symbiotic relationship between microalgae and fungi is selective. Only suitable strains can grow together and act together in sewage treatment. Unsuitable fungi have an algae-lytic effect, and the algal cells will grow with the extension of the co-cultivation time. and decline. Therefore, if a fungal-microalgae symbiosis system suitable for the treatment of glucosamine processing wastewater can be constructed, it is of great significance to promote the processing and production of GlcN.

SUMMARY OF THE INVENTION

In order to solve the above-mentioned technical problems, the present invention proposes a method for using a fungal-microalgae symbiosis system to process wastewater from glucosamine processing. The wastewater from glucosamine processing is biologically treated, especially inorganic chloride ions therein. On the basis of the method for treatment, a green and environmental protection treatment method is added to solve the problem of secondary pollution; and the present invention uses two filamentous fungi and one microalgae to form a symbiotic system to treat the wastewater, so as to solve the problem that the suspension of bacteria micelles hinders the sewage Addresses issues with the normal operation of the facility.

For achieving the above object, the present invention provides the following scheme:

The invention provides a method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

(1) Microalgae pre-cultivation: Euglena gracilis var. saccharophila was cultured in Hunter medium three months before use, and then transferred to glucosamine processing wastewater for culture to obtain algal cells;

(2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, and then transferred to new G-YPD medium for several times. Transfer culture, and finally use sterile 0.05% Tween solution to collect two fungal spores;

(3) construct algal-bacteria symbiosis system: put two kinds of fungal spores obtained in step (2) into glucosamine processing wastewater, add the algal cells obtained in step (1) after outdoor culture, start aeration simultaneously, and When co-cultivation is carried out, when the aggregated microspheres with a diameter of 6-10 mm are visible to the naked eye in the reaction tank, the aeration is stopped after that, the algal microspheres are allowed to sink to the bottom, the supernatant is discharged, and new glucosamine processing wastewater is continuously injected to continue the cultivation. And carry out 3 to 4 cycles to obtain an algae-bacteria symbiosis system; the algae-bacteria symbiosis system is easy to construct, operates stably, and can be transferred multiple times, and the concentrated algae and bacteria can be stored separately at low temperature when idle to avoid regular investment required for maintenance.

(4) Pretreatment of glucosamine processing wastewater: After the glucosamine processing wastewater is treated by grids, screens and grit chambers, the pH is adjusted to be between 4 and 6, and then pre-aerated for 3 to 5 hours to obtain pretreated wastewater. Glucosamine processing wastewater;

(5) Biological treatment of glucosamine processing wastewater: inject the pretreated glucosamine processing wastewater obtained in step (4) into the reaction tank containing the algal-bacterial symbiosis system prepared in step (3), perform aeration, and then stop aeration to make ammonia The sugar processing wastewater is drained to the sedimentation tank through the filter screen (ф5mm), and then the supernatant liquid is drained to the secondary sedimentation tank through the filter screen (ф2mm), and the algaecide is added to the secondary sedimentation tank, stir evenly, and take the overflow. It can realize the treatment of glucosamine processing wastewater.

Further, in step (1), the total number of Euglena slender cells is 1×10 ⁹ to 1.7×10 ⁹ added to 200 ml of Hunter culture solution, and the culture condition in the Hunter culture solution is normal temperature light shock culture, and the culture time is 3 to 4 sky.

Further, in step (1), the concrete steps of transferring into glucosamine processing wastewater and culturing are as follows: after culturing in Hunter nutrient solution for 3 to 4 days, transfer 100 ml of glucosamine processing wastewater, and then add 100 ml to the culture system every two weeks. For glucosamine processing wastewater, when the volume of the culture solution reaches 600ml, transfer all the culture solution into a 10L large-mouth glass jar container, add the glucosamine processing wastewater to the mark, and culture it outdoors for 3 days with sealing and ventilation to obtain algal cells.

Further, step (2) is specifically as follows: inoculate black mold (Aspergillus niger) and Mucorhiemalis (Mucorhiemalis) in G-YPD medium for 7 to 9 days at room temperature, and then transfer to a new G-YPD medium , for each transfer, the nutrient components of the original YPD medium are reduced by 12% to 20%, until the final plate medium contains only two components of ammonia sugar processing wastewater and agar, and finally use sterile 0.05% Tween solution to collect the two components Fungal spores.

Further, the G-YPD medium contains yeast extract 1% (w/v), peptone 2% (w/v), glucose 2% (w/v), agar powder 2% (w/v) ), which is prepared by sterilizing and sterilizing the components after mixing and suspending the various components in the waste water of glucosamine processing.

Further, in step (3), the dosage of black mold into the glucosamine processing wastewater is (5.0×10 ¹⁰ ~ 6.0×10 ¹⁰ ) pieces/cubic meter, and the dosage of mucor mold is about (1.0×10 ¹² ) ～1.6×10 ¹² )/cubic meter, and the dosage of algal cells is (1×10 ¹² ～1.5×10 ¹² )/cubic meter.

Further, in step (3), two kinds of fungal spores are added for outdoor cultivation for 4-6 days, the aeration is a daily interval of 10-12 hours, and the number of days of the co-cultivation is 10-14 days.

Further, in step (5), the algal bacterial symbiosis system is added to the pretreated glucosamine processing wastewater so that the concentration of the algal bacterial microspheres is 5-6.2 g/L.

Further, in step (5), the time that each batch of pretreated glucosamine processing wastewater stays in the reaction tank is 8 to 10 hours, the time to stay in the sedimentation tank is 5 to 8 hours, and the time to stay in the secondary sedimentation tank is 12～16h.

Further, the concentration of the algicide potassium monopersulfate in step (5) is 0.01-0.06 ppm.

Further, the indexes such as suspended solids, Cl ⁻ , COD _Cr , BOD ₅ , pH and the like in the overflow liquid described in step (5) all meet the discharge standard for extracting pharmaceutical industry water pollutants (GB21905-2008).

The present invention discloses the following technical effects:

(1) The present invention forms algal microspheres by constructing a symbiotic system of microalgae and fungi, which not only ensures the effect of sewage treatment, but also enables the natural settlement of algal colonies, reduces the processing capacity of centrifugal recovery, and simultaneously avoids suspended colonies of colonies. pipe blockage problem.

(2) In the present invention, the algae-bacteria symbiosis system is used to directly treat the wastewater of ammonia sugar processing, replacing the current process of first chemical treatment and then biological treatment, so as to avoid the problem of secondary pollution caused by the use of chemical reagents.

(3) The glucosamine processing wastewater obtained by the method of the present invention complies with the extraction standard for the discharge of water pollutants in the pharmaceutical industry (GB 21905-2008).

Detailed ways

Various exemplary embodiments of the present invention will now be described in detail, which detailed description should not be construed as a limitation of the invention, but rather as a more detailed description of certain aspects, features, and embodiments of the invention.

It should be understood that the terms described in the present invention are only used to describe particular embodiments, and are not used to limit the present invention. Additionally, for numerical ranges in the present disclosure, it should be understood that each intervening value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range, and any other stated value or intervening value in that stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention relates. Although only the preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials in connection with which the documents are referred. In the event of conflict with any incorporated document, the content of this specification controls.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present invention without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the present invention. The description and examples of the present invention are exemplary only.

As used herein, "comprising," "including," "having," "containing," and the like, are open-ended terms, meaning including but not limited to.

The Euglena slenderum used in the examples of the present invention was purchased from the algae library of the University of Texas (No. UTEX 752), and the black mold and mucor mold were both isolated and purified by the inventor from mangrove wetlands, and the methods of isolation and purification were the same as The conventional technical means in the field are not regarded as invention points, so they will not be described in detail.

The G-YPD medium, Hunter culture solution, Tween solution and the algaecide potassium monopersulfate used in the practice of the present invention are all purchased related components, prepared by the inventor, and the components of the above solution are all components of those skilled in the art. I know, I won't go into too much detail.

The glucosamine processing wastewater used in the embodiment of the present invention was obtained from Fujian Huakang Pharmaceutical Co., Ltd.

The specific preparation steps of the G-YPD medium used in the embodiment of the present invention are as follows: taking the preparation of 1 L of medium as an example, suspend 10 g of yeast extract, 20 g of peptone, and 20 g of agar powder in 900 ml of glucosamine processing wastewater, and sterilize at 121° C. for 15 minutes at high pressure. Another 20 g of glucose was dissolved in 60 mL of glucosamine processing wastewater, and sterilized by filtration with a 0.22 μm filter membrane. Mix the two sterilization liquids, add sterilized glucosamine processing wastewater to 1L.

The technical solutions of the present invention will be further illustrated by the following examples.

Example 1

A method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

(1) Microalgae pre-culture: Euglena gracilis var. saccharophila was cultured in a 2000ml shake flask with 200ml of Hunter medium three months before use, so that the total number of Euglena gracilis var. saccharophila cells was 1×10 ⁹ , cultured under normal temperature and light for 3 days, then transferred to 100ml of ammonia sugar processing wastewater, and then added 100ml of ammonia sugar processing wastewater to the culture system every two weeks. When the volume of the culture solution reached 600ml, all the culture solution was transferred to In a 10L large-mouth glass jar container, add ammonium sugar processing wastewater to the mark, and seal it outdoors for 3 days and cultivate it for 3 days to obtain algal cells;

(2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, cultured at room temperature for 8 days, and then transferred to a new G-YPD culture In the base, the nutrient components of the original YPD medium are reduced by 20% for each transfer, until the final plate medium contains only two components of glucosamine processing wastewater and agar, and finally the two fungi are collected using sterile 0.05% Tween solution. spore;

(3) Construct algae-bacteria symbiosis system: inject the slagging and primary precipitation ammonia sugar processing wastewater into the open reaction tank, put the two kinds of fungal spores obtained in step (2) into the ammonia sugar processing wastewater, and throw the black mold into the wastewater. The dosage is 5.0×10 ¹⁰ /cubic meter, the dosage of Mucor mold is about 1.0×10 ¹² /cubic meter, cultivated outdoors for 5 days, and then add the algal cells obtained in step (1). The added amount is 1.5×10 ¹² /m3, and the aeration is started at the same time, and the aeration is carried out at intervals of 12 hours every day, and co-cultivation is carried out for 12 days. The algal bacteria microspheres sink to the bottom, discharge the supernatant, continue to inject new glucosamine processing wastewater, continue to cultivate and carry out 4 cycles to obtain the algal bacteria symbiosis system;

(4) Pretreatment of glucosamine processing wastewater: After the glucosamine processing wastewater is treated by grids, screens and grit chambers, the pH is adjusted to 4, and then pre-aerated for 3 hours to obtain pretreated glucosamine processing wastewater;

(5) Biological treatment of glucosamine processing wastewater: inject the pretreated glucosamine processing wastewater obtained in step (4) into the reaction tank, add the algal-bacteria symbiosis system prepared in step (3), and make the algal-bacteria symbiotic system after the algal-bacteria symbiotic system The concentration of the ball is 5g/L, and aeration is carried out, so that the pretreated wastewater from glucosamine processing stays in the reaction tank for 9 hours, then the aeration is stopped, and the wastewater from glucosamine processing is drained through the filter screen (ф5mm) within 4 hours. Go to the conical sedimentation tank, stay for 6 hours, then drain the supernatant to the secondary sedimentation tank through a filter (ф2mm), and add the algicidal fungicide potassium monopersulfate to the secondary sedimentation tank, stir evenly, and stay for 15h , the treatment of glucosamine processing wastewater can be realized by taking the overflow liquid.

Example 2

A method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

(1) microalgae pre-cultivation: with embodiment 1;

(2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, cultured at room temperature for 9 days, and then transferred to a new G-YPD culture In the base, the nutrient components of the original YPD medium are reduced by 20% for each transfer, until the final plate medium contains only two components of glucosamine processing wastewater and agar, and finally the two fungi are collected using sterile 0.05% Tween solution. spore;

(3) Construct algae-bacteria symbiosis system: inject the slagging and primary precipitation ammonia sugar processing wastewater into the open reaction tank, put the two kinds of fungal spores obtained in step (2) into the ammonia sugar processing wastewater, and throw the black mold into the wastewater. The dosage is 5.5×10 ¹⁰ /cubic meter, and the dosage of Mucor mold is about 1.3×10 ¹² /cubic meter. After 4 days of outdoor culture, the algal cells obtained in step (1) are added. The added amount is 1.25×10 ¹² /m3, and the aeration is started at the same time, and the aeration is carried out at intervals of 12 hours every day, and co-cultivation is carried out for 14 days. The algal bacteria microspheres sink to the bottom, discharge the supernatant, continue to inject new glucosamine processing wastewater, continue to cultivate and carry out 3 cycles to obtain the algal bacteria symbiosis system;

(4) Pretreatment of glucosamine processing wastewater: After the glucosamine processing wastewater is treated with grids, screens and grit chambers, the pH is adjusted to pH 5, and then pre-aerated for 4 hours to obtain pretreated glucosamine processing wastewater;

(5) Biological treatment of glucosamine processing wastewater: inject the pretreated glucosamine processing wastewater obtained in step (4) into the reaction tank, add the algal-bacteria symbiosis system prepared in step (3), and make the algal-bacteria symbiotic system after the algal-bacteria symbiotic system The concentration of the balls is 5.6g/L, and aeration is carried out to make the pretreated wastewater from glucosamine processing stay in the reaction tank for 8 hours. After that, the aeration is stopped, and the wastewater from glucosamine processing is discharged through a filter (ф5mm) within 3 hours. Dry into the conical sedimentation tank, stay for 5h, then drain the supernatant through the filter (ф2mm) to the secondary sedimentation tank, and add the algicidal fungicide potassium monopersulfate to the secondary sedimentation tank, stir evenly, and stay 16h, taking the overflow liquid can realize the treatment of glucosamine processing wastewater.

Example 3

A method for utilizing a fungal-microalgae symbiotic system to process wastewater from glucosamine processing, comprising the following steps:

(1) microalgae pre-cultivation: with embodiment 1;

(2) Fungal pre-culture: Aspergillus niger and Mucor hiemalis were inoculated into G-YPD medium three months before use, cultured at room temperature for 7 days, and then transferred to a new G-YPD culture In the base, the nutrient components of the original YPD medium are reduced by 20% for each transfer, until the final plate medium contains only two components of glucosamine processing wastewater and agar, and finally the two fungi are collected using sterile 0.05% Tween solution. spore;

(3) Construct algae-bacteria symbiosis system: inject the slagging and primary precipitation ammonia sugar processing wastewater into the open reaction tank, put the two kinds of fungal spores obtained in step (2) into the ammonia sugar processing wastewater, and throw the black mold into the wastewater. The dosage is 6.0×10 ¹⁰ /cubic meter, and the dosage of Mucor mold is about 1.6×10 ¹² /cubic meter. After 6 days of outdoor cultivation, the algal cells obtained in step (1) are added. The addition amount is 1.5×10 ¹² /m3, and the aeration is started at the same time, and the aeration is carried out at intervals of 12 hours every day, and they are co-cultured for 10 days. The algal bacteria microspheres sink to the bottom, discharge the supernatant, continue to inject new glucosamine processing wastewater, continue to cultivate and carry out 4 cycles to obtain the algal bacteria symbiosis system;

(4) Pretreatment of glucosamine processing wastewater: After the wastewater from glucosamine processing is treated with grids, screens and grit chambers, the pH is adjusted to a pH of 5.2, and then pre-aerated for 3 hours to obtain pretreated glucosamine processing wastewater ;

(5) Biological treatment of glucosamine processing wastewater: inject the pretreated glucosamine processing wastewater obtained in step (4) into the reaction tank, add the algal-bacteria symbiosis system prepared in step (3), and make the algal-bacteria symbiotic system after the algal-bacteria symbiotic system The concentration of the ball is 6.2g/L, and aeration is carried out to make the pretreated wastewater from glucosamine processing stay in the reaction tank for 10 hours. After that, the aeration is stopped, and the wastewater from glucosamine processing is discharged through the filter screen (ф5mm) within 2 hours. Dry into the conical sedimentation tank, stay for 8h, then drain the supernatant through the filter (ф2mm) to the secondary sedimentation tank, and add the algicidal fungicide potassium monopersulfate to the secondary sedimentation tank, stir evenly, and stay 12h, taking the overflow liquid can realize the treatment of glucosamine processing wastewater.

Result test of ammonia sugar processing wastewater treatment

The overflow liquid obtained by the treatment of Examples 1 to 3 and the untreated glucosamine processing wastewater are compared for the indexes of suspended solids, Cl ^- , COD _Cr , BOD ₅ , and pH. The standards on which each index is detected are respectively: suspended solids According to GB/T 11901-1989; Cl ^- according to GB/T11896-1989; COD _Cr according to GB/T 11901-1989; BOD ₅ according to GB/T 7488-1987; pH according to GB/T6920-1986, the results are shown in Table 1.

Table 1 Test results (unit: mg/L)

As can be seen from the content of Table 1, the suspended solids, ^Cl- , COD _Cr , BOD ₅ and pH indicators in the glucosamine processing wastewater processed by the method of Example 1, 2 or 3 all meet the extraction standard for the discharge of water pollutants in the pharmaceutical industry ( GB 21905-2008), and in the process of wastewater treatment, there is no pipeline blockage caused by bacterial suspension, and the algal-bacteria symbiosis system is used to directly treat the wastewater from ammonia sugar processing, replacing the current process of chemical treatment and biological treatment. Avoid secondary pollution problems caused by the use of chemical reagents.

The above-mentioned embodiments are only to describe the preferred modes of the present invention, but not to limit the scope of the present invention. Without departing from the design spirit of the present invention, those of ordinary skill in the art can make various modifications to the technical solutions of the present invention. Variations and improvements should fall within the protection scope determined by the claims of the present invention.

Claims (8)

1. a method utilizing fungal microalgae symbiotic system to process glucosamine processing wastewater, is characterized in that, comprises the following steps: (1) Microalgae pre-cultivation: Euglena slenderness is cultivated in Hunter trace element culture solution (Hunter culture solution), and then transferred to glucosamine processing wastewater and cultured to obtain algal cells; (2) Fungal pre-cultivation: Black mold and Mucor mold were inoculated into yeast extract peptone glucose medium (G-YPD) prepared from glucosamine processing wastewater, and then transferred to a new G-YPD medium. Second transfer culture, and finally use sterile 0.05% Tween solution to collect two kinds of fungal spores; (3) Construct an algal-bacterial symbiosis system: put two kinds of fungal spores obtained in step (2) into the waste water of glucosamine processing, add the algal cells obtained in step (1) after outdoor cultivation, start aeration at the same time, and carry out Co-cultivation, then stop aeration, make the algal bacteria microspheres sink to the bottom, discharge the supernatant, continue to inject new ammonia sugar processing wastewater, continue to cultivate and circulate to obtain the algal bacteria symbiosis system; (4) Pretreatment of glucosamine processing wastewater: after the glucosamine processing wastewater is treated by grids, screens and grit chambers, the pH is adjusted, and then pre-aerated to obtain pretreated glucosamine processing wastewater; (5) Biological treatment of glucosamine processing wastewater: inject the pretreated glucosamine processing wastewater obtained in step (4) into the reaction tank containing the algal-bacterial symbiosis system prepared in step (3), perform aeration, and then stop aeration to make ammonia The sugar processing wastewater is drained to the sedimentation tank through the filter screen, and then the supernatant liquid is drained to the secondary sedimentation tank through the filter screen. Treatment of sugar processing wastewater. 2 . The method for treating glucosamine processing wastewater according to claim 1 , wherein the total number of Euglena slender cells added to 200 ml of Hunter culture solution in step (1) is 1×10 ⁹ to 1.7×10 ⁹ . The culture condition in the culture medium is normal temperature light shock culture, and the culture time is 3-4 days. 3. the method for processing glucosamine processing wastewater according to claim 1, is characterized in that, in step (1), the concrete step that is transferred into glucosamine processing wastewater and cultivated is: after culturing in Hunter nutrient solution for 3～4 days, transfer Add 100ml of glucosamine processing wastewater, then add 100 ml of glucosamine processing wastewater to the culture system every two weeks, when the volume of the culture solution reaches 600ml, transfer all the culture solution into a 10L container, add glucosamine processing wastewater to the mark, The algal cells can be obtained by culturing with aeration outdoors. 4. the method for processing glucosamine processing wastewater according to claim 1 is characterized in that, step (2) is specially: inoculate black mold and mucor mold in G-YPD substratum, cultivate 7～9 days at room temperature, After transferring to new G-YPD medium, the nutrient components in the original YPD medium were reduced by 12% to 20% for each transfer, until the final plate medium only contained two groups of glucosamine processing wastewater and agar. The two fungal spores were finally collected using sterile 0.05% Tween solution. 5. The method for treating glucosamine processing wastewater according to claim 1, characterized in that, in step (3), the dosage of black mold into the glucosamine processing wastewater is (5.0×10 ¹⁰ ～6.0×10 ¹⁰ ). )/cubic meter, the dosage of Mucor fungi is about (1.0×10 ¹² ～ 1.6×10 ¹² )/cubic meter, and the dosage of algal cells is (1×10 ¹² ～1.5×10 ¹² )/cubic Meter. 6. The method for treating glucosamine processing wastewater according to claim 1, wherein in step (3), two kinds of fungal spores are added for outdoor cultivation for 4-6 days, and the aeration is a daily interval aeration of 10-12h , the co-cultivation days are 10-14 days. 7. The method for processing glucosamine processing wastewater according to claim 1, characterized in that in step (5), after adding the algal bacteria symbiotic system to the pretreated glucosamine processing wastewater, the algal bacteria microsphere concentration is 5～5～ 6.2g/L. 8. the method for processing glucosamine processing wastewater according to claim 1, is characterized in that, in step (5), the time that each batch of pretreated glucosamine processing wastewater stays in the reaction tank is 8～10h, in the sedimentation tank The residence time in the secondary sedimentation tank is 5 to 8 hours, and the residence time in the secondary sedimentation tank is 12 to 16 hours.