CN106587197A - Nano biological cycle water treatment agent - Google Patents

Nano biological cycle water treatment agent Download PDF

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CN106587197A
CN106587197A CN201611182541.6A CN201611182541A CN106587197A CN 106587197 A CN106587197 A CN 106587197A CN 201611182541 A CN201611182541 A CN 201611182541A CN 106587197 A CN106587197 A CN 106587197A
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culture medium
parts
bacterium
bacterium solution
culture
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CN106587197B (en
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薛小虎
刘成柱
黄超
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Jiangsu Green Environmental Protection Technology Co Ltd
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Jiangsu Green Environmental Protection Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
    • C02F1/5245—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using basic salts, e.g. of aluminium and iron
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
    • C02F1/5254—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using magnesium compounds and phosphoric acid for removing ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The invention relates to a nano biological cycle water treatment agent which comprises the following raw materials in parts by mass: 10-20 parts of sepiolite fibers, 100-150 parts of pure diatom, 10-20 parts of volcanic carbon, 60-100 parts of attapulgite, 6-10 parts of zeolite, 10-20 parts of an enzymatic agent, 0.02-0.07 part of magnesium hydroxide, 0.02-0.07 part of ferric oxide, 30-50 parts of glucose, 50-80 parts of an aerobic bacterium agent, 20-30 parts of an anaerobic bacterium agent, 10-20 parts of a nitrifying bacterium agent, 10-20 parts of a denitrifying bacterium agent, 10-20 parts of a phosphorus-accumulating bacterium agent and 10-20 parts of a hydrolytic acidification bacterium agent. The preparation method comprises the following steps: sufficiently mixing various materials which are weighed according to a mass ratio, and performing packaging, thereby obtaining a finished product. The water treatment agent can be applied to treatment of various types of hardly-degradable industrial wastewater and has excellent effects of degrading and removing multiple pollutants, and the moisture content of sludge can be reduced.

Description

Nano biological circulating water treatment agent
Technical field
The present invention relates to effluent cycle inorganic agent.
Background technology
At present, countries in the world water consumption sharply increase, water resources crisis it is increasingly serious, while various environmental regulations（Water is dirty Contaminate anti-method for the treatment of）Formulate in succession, and it is increasingly stricter, therefore especially the quick of sewage disposal technology sends out to promote green technology Exhibition.Water treatment agent play the role of in water treatment procedure it is very important, although water technology has biochemical process, Physical, physics Chemical method, and constantly have new technology and Technical investment market, but be all difficult to substitute chemical agent completely.Therefore, water treatment agent and Water treatment facilities are known as two wings of water treatment industry.
As the fast-developing and living standards of the people of China's process industry are greatly improved, water resource excessively develops profit Also increasingly severe with, the pollution that causes to natural water body of commercial production and sanitary sewage, water pollutant composition is also more next It is more complicated, including Some Organic Pollutants, heavy metal element, high concentration salts etc..Although having some water treatment agents at present Come into operation, such as coagulant, flocculation aid, but merely by these medicaments add can not meet present water process will Ask, especially for difficult de- steady colloid, ammonia nitrogen, heavy metal ion excessive problem, its treatment effect is unsatisfactory.Meanwhile, some Water treatment agent can produce secondary pollution during production and use, increase the harmful components in water body.Therefore, research and develop a kind of High efficiency, applied range, environmental protection, safe sewage-treating agent are very necessary.
The content of the invention
It is an object of the invention to provide a kind of nano biological circulating water treatment agent, can be recycled, it is suitable for extensive Property, non-secondary pollution reduces sludge moisture content.
The present invention the adopted technical scheme that solves the above problems is：A kind of nano biological circulating water treatment agent, raw material matter Measuring proportioning is：Sepiolite fibre, 10-20 parts；Pure silicon algae, 100-150 parts；Volcanic rock charcoal, 10-20 parts；Attapulgite, 60-100 Part；Zeolite, 6-10 parts；Growth-promoting enzyme agent, 10-20 parts；Magnesium hydroxide, 0.02-0.07 parts；Ferrum oxide, 0.02-0.07 parts；Fructus Vitis viniferae Sugar, 30-50 parts；Aerobic microbial inoculum 50-80 parts；Anaerobism microbial inoculum, 20-30 parts；Nitrification microbial inoculum, 10-20 parts；Denitrification microbial inoculum, 10-20 Part；PolyP bacteria agent, 10-20 parts；Hydrolysis acidification microbial inoculum, 10-20 parts；Above-mentioned each material that example in mass ratio is measured is sufficiently mixed Afterwards packaging is finished product.
The principle of each raw material configuration of the application and effect
Sepiolite fibre, volcanic rock charcoal, attapulgite, zeolite etc. can be effectively gone in eliminating water with performances such as good absorption, filtrations Turbidity, colourity, heavy metal ion, Organic substance, oils and other polluters；Pure silicon algae not only has absorption well, filters Performance, while the flocculation of pollutant, precipitation can be promoted, and can be effectively improved the property of sludge, make what is formed in water treatment procedure Sludge has good dewatering, reduces the water content of sludge, no longer needs additionally to add Treatment of Sludge medicine in subsequent treatment Agent, reduces follow-up Treatment of Sludge cost；Magnesium hydroxide and ferrum oxide can strengthen flocculation sediment function；Aerobic bacteria, anaerobe, hydrolysis Acidifying bacterium etc. acts on the polluter in degradation water by microbial metabolism, and nitrifier, denitrifying bacterium are mainly used in denitrogenation, poly- phosphorus Bacterium is mainly used in dephosphorization；Glucose provides energy for microbial metabolism；The agent of growth-promoting enzyme is used to promote microbial metabolism to act on.
Sepiolite fibre：Meerschaum composition ＞ 85%, granularity：250～300 mesh, 2～2.5g/cm of density3, CaO ＜ 1.5%, Fe2O3＜ 0.03%；
Pure silicon algae：SiO2 >=88, true density 2.3g/cm3, the g/cm of bulk density 0.20～0.303, fineness：100～500 mesh；
Volcanic rock charcoal；Particle diameter：1-2mm, density 0.75g/cm3；
Attapulgite：Active substance content >=90%, granularity：100～200 mesh, heap 0.5 ± 1g/cm of density3, water content≤8%；
Zeolite：Clinoptilolite, granularity：180～200 mesh, density 1.92g/cm3, ammonia absorption value ＞ 100mg equivalents/100g, moisture≤ 1.8%。
The preparation method of above-mentioned aerobic microbial inoculum, step is as follows,
（1）Aerobic bacteria strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
Aerobic bacteria strain includes Bacillus licheniformis, Pseudomonas aeruginosa, Nitrosomonass, belleville sulfur bacterium, each strain list Solely culture, obtains corresponding bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Lichens 2% part of bacillus cereuss, 3% part of Pseudomonas aeruginosa, 2% part of Nitrosomonass, 4% part of belleville sulfur bacterium；Mixed culture temperature 25～30 DEG C, incubation time 24～48 hours obtains aerobic bacteria mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained aerobic bacteria bacterium solution is by being dried to obtain aerobic microbial inoculum.It is dried using centrifugation drying or lyophilization.
The preparation method of above-mentioned anaerobism microbial inoculum, step is as follows,
（1）Anaerobe strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
Anaerobe strain includes methane phase bacillus, methane phase coccus, desulfovibrio, and each strain single culture obtains corresponding Bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Produce first 2% part of alkane bacillus, 3% part of methane phase coccus, 2% part of desulfovibrio；25～30 DEG C of temperature of mixed culture, incubation time 24 ～48 hours, obtain anaerobe mixed bacteria liquid.The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, Portugal Grape sugar 0.5%, NH4Cl20.5%th, balance of water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained anaerobe bacterium solution is by being dried to obtain anaerobism microbial inoculum.
The preparation method of above-mentioned nitrification microbial inoculum, step is as follows,
（1）Nitrifier strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains nitrifier bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, it is balance of Water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained nitrifier bacterium solution is by being dried to obtain nitrification microbial inoculum.
The preparation method of above-mentioned denitrification microbial inoculum, step is as follows,
（1）Denitrifying bacterium strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast Cream 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium is through 121 DEG C 20 points Clock high temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains denitrifying bacterium bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, it is balance of Water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained denitrifying bacterium bacterium solution is by being dried to obtain denitrification microbial inoculum.
The preparation method of above-mentioned polyP bacteria agent, step is as follows,
（1）PolyP bacteria strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains polyP bacteria bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, it is balance of Water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained polyP bacteria bacterium solution is by being dried to obtain polyP bacteria agent.
The preparation method of above-mentioned hydrolysis acidification microbial inoculum, step is as follows,
（1）Hydrolysis acidification bacterium strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Ferment Female cream 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium is through 121 DEG C 20 Minute high temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
Hydrolysis acidification bacterium strain includes Cellumomonas flavigena, starch Clostridium, Bacillus cercuses, succinic acid bacteroid, Each strain single culture, obtains corresponding bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Produce yellow 2% part of cellulomonas cartae, 3% part of starch Clostridium, 2% part of Bacillus cercuses, 4% part of succinic acid bacteroid；Mixing 25～30 DEG C of cultivation temperature, incubation time 24～48 hours obtains hydrolysis acidification bacterium mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained hydrolysis acidification bacterium bacterium solution is by being dried to obtain hydrolysis acidification microbial inoculum.
Growth-promoting enzyme according to the present invention agent includes 1～2 part of organic carbon source, 1～2 part of organic nitrogen source, compound dimension life per 10 parts Element 0.5～1 part, 1～2 part of organic acid remaining as enzyme, the organic acid selected from citric acid, lactic acid, acetic acid, gluconic acid, One or more in malic acid, kojic acid, propanoic acid, succinic acid, ascorbic acid, salicylic acid；The enzyme be protease, Lipase or cellulase.The agent of growth-promoting enzyme provides nutrient substance for microorganism, promotes the growth breeding of microorganism, and can improve micro- Biological activity, allow microorganism in relatively rugged environment can rapid, high volume growth and breeding, form good zoogloea, improve The degradation capability of microbe.
Compared with prior art, it is an advantage of the current invention that：
（1）Set multiple technologies principle, applied range.Some water treatment agent know-why, ranges of application in the market It is relatively simple, and treatment effect is not very good.This product in combination with biological, physics and chemical degradation technology, no Only with the addition of the professional mushroom composition for being adapted to the various property waste water of degraded, but also with strengthen chemical reaction, adsorption reaction with And the effect of flocculation sediment.Can be applicable to all kinds of indegradable industrial effluents to process, and have excellent drop to multiple pollutant matter Solution and removal effect.
（2）Green Water Treatment Reagents, non-secondary pollution.There is in the market the water treatment agent such as polyacrylamide of similar functions Amine, aluminium polychlorid, all kinds of disinfectant etc., may be containing heavy metal element, chlorine residue, poisonous during production process and use Organic solution and various harmful derivants.This product is safe and reliable, stable performance, will not produce secondary pollution, belongs to green The technology category of water treatment agent, meets the development trend of current Innovation ＆ environment water treatment agent.
（3）Achievable sludge reduction.A large amount of generations of municipal sewage plant excess sludge are the another of current field of environment protection A difficult problem, the sludge dewatering produced in general water treatment process is very difficult, and sludge moisture content is very high, increased the place of sludge Reason difficulty, process needs to add medicament, and there is a problem of secondary pollution.If adding the water process of the application in processing procedure Agent, can accelerate flocculation sediment speed, sludge is quickly condensed, and improve dewatering, realize mud decrement 5%～10%, and sludge In do not exist because adding water treatment agent the harmful substance that produces, any chemical agent need not be added in sludge handling process.
（4）Recycle, reducing energy consumption.Some water treatment agent products are heavy dose of consumable goodss, such as various flocculant, sterilization Agent etc., constantly consumes during use, and needs continue quantitative addition.This product can be recycled after once adding, and use A small amount of consumption is only existed in journey, only a small amount of medicament need to be by phased manner supplemented, is reached and is ensured the same of quality of water treatment requirement When reduce cost of water treatment and energy-saving purpose.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Biological cycle water treatment agent according to the present invention, main component include sepiolite fibre, pure silicon algae, volcanic rock charcoal, Attapulgite, zeolite, growth-promoting enzyme agent, magnesium hydroxide, ferrum oxide, glucose, aerobic microbial inoculum, anaerobism microbial inoculum nitrifies microbial inoculum, denitrification Microbial inoculum, polyP bacteria agent, hydrolysis acidification microbial inoculum.
The concrete proportioning raw materials of each embodiment are shown in Table 1.
The water treatment agent component list of table 1（Weight portion）
The preparation method of the application nano biological circulating water treatment agent, is that each raw material that will be prepared is sufficiently mixed uniformly, and packaging is Can, direct plunge in sewage when using.
Aerobic microbial inoculum in embodiment, anaerobism microbial inoculum nitrifies microbial inoculum, denitrification microbial inoculum, polyP bacteria agent, hydrolysis acidification microbial inoculum Concrete cultural method it is as follows
First, the preparation method of aerobic microbial inoculum, step is as follows,
（1）Aerobic bacteria strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, 28 DEG C of cultivation temperature, incubation time 36 hours；
Aerobic bacteria strain includes Bacillus licheniformis, Pseudomonas aeruginosa, Nitrosomonass, belleville sulfur bacterium, each strain list Solely culture, obtains corresponding bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Lichens 2% part of bacillus cereuss, 3% part of Pseudomonas aeruginosa, 2% part of Nitrosomonass, 4% part of belleville sulfur bacterium；Mixed culture temperature 25～30 DEG C, incubation time 24～48 hours obtains aerobic bacteria mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained aerobic bacteria bacterium solution is dried using centrifugation drying or lyophilization by being dried to obtain aerobic microbial inoculum.
2nd, the preparation method of anaerobism microbial inoculum, step is as follows,
（1）Anaerobe strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, 28 DEG C of cultivation temperature, incubation time 36 hours；
Anaerobe strain includes methane phase bacillus, methane phase coccus, desulfovibrio, and each strain single culture obtains corresponding Bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Produce first 2% part of alkane bacillus, 3% part of methane phase coccus, 2% part of desulfovibrio；25～30 DEG C of temperature of mixed culture, incubation time 24 ～48 hours, obtain anaerobe mixed bacteria liquid.The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, Portugal Grape sugar 0.5%, NH4Cl20.5%th, balance of water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained anaerobe bacterium solution is by being dried to obtain anaerobism microbial inoculum.
3rd, the preparation method of microbial inoculum is nitrified, step is as follows,
（1）Nitrifier strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, 28 DEG C of cultivation temperature, incubation time 36 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains nitrifier bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, it is balance of Water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained nitrifier bacterium solution is by being dried to obtain nitrification microbial inoculum.
4th, the preparation method of denitrification microbial inoculum, step is as follows,
（1）Denitrifying bacterium strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast Cream 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium is through 121 DEG C 20 points Clock high temperature sterilize, 28 DEG C of cultivation temperature, incubation time 36 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains denitrifying bacterium bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, it is balance of Water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained denitrifying bacterium bacterium solution is by being dried to obtain denitrification microbial inoculum.
5th, the preparation method of polyP bacteria agent, step is as follows,
（1）PolyP bacteria strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, 28 DEG C of cultivation temperature, incubation time 36 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains polyP bacteria bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, it is balance of Water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained polyP bacteria bacterium solution is by being dried to obtain polyP bacteria agent.
6th, the preparation method of hydrolysis acidification microbial inoculum, step is as follows,
（1）Hydrolysis acidification bacterium strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Ferment Female cream 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium is through 121 DEG C 20 Minute high temperature sterilize, 28 DEG C of cultivation temperature, incubation time 36 hours；
Hydrolysis acidification bacterium strain includes Cellumomonas flavigena, starch Clostridium, Bacillus cercuses, succinic acid bacteroid, Each strain single culture, obtains corresponding bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Produce yellow 2% part of cellulomonas cartae, 3% part of starch Clostridium, 2% part of Bacillus cercuses, 4% part of succinic acid bacteroid；Mixing 25～30 DEG C of cultivation temperature, incubation time 24～48 hours obtains hydrolysis acidification bacterium mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained hydrolysis acidification bacterium bacterium solution is by being dried to obtain hydrolysis acidification microbial inoculum.
The wastewater treatment efficiency of circulating water treatment agent of the present invention, is shown in Table 2
Process test is carried out to town-sewage factory biochemistry pool waste water, it is as a result as shown in the table：
In addition to the implementation, present invention additionally comprises there is other embodiment, all employing equivalents or equivalence replacement mode The technical scheme of formation, all should fall within the scope of the hereto appended claims.

Claims (9)

1. a kind of nano biological circulating water treatment agent, it is characterised in that：Raw material mass mixture ratio is：Sepiolite fibre, 10-20 parts； Pure silicon algae, 100-150 parts；Volcanic rock charcoal, 10-20 parts；Attapulgite, 60-100 parts；Zeolite, 6-10 parts；Growth-promoting enzyme agent, 10-20 Part；Magnesium hydroxide, 0.02-0.07 parts；Ferrum oxide, 0.02-0.07 parts；Glucose, 30-50 parts；Aerobic microbial inoculum 50-80 parts；Detest Oxygen microbial inoculum, 20-30 parts；Nitrification microbial inoculum, 10-20 parts；Denitrification microbial inoculum, 10-20 parts；PolyP bacteria agent, 10-20 parts；Hydrolysis acidification Microbial inoculum, 10-20 parts；
Packaging is finished product after above-mentioned each material that example in mass ratio is measured is sufficiently mixed.
2. nano biological circulating water treatment agent according to claim 1, it is characterised in that：The preparation side of the aerobic microbial inoculum Method, step is as follows,
（1）Aerobic bacteria strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
Aerobic bacteria strain includes Bacillus licheniformis, Pseudomonas aeruginosa, Nitrosomonass, belleville sulfur bacterium, each strain list Solely culture, obtains corresponding bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Lichens 2% part of bacillus cereuss, 3% part of Pseudomonas aeruginosa, 2% part of Nitrosomonass, 4% part of belleville sulfur bacterium；Mixed culture temperature 25～30 DEG C, incubation time 24～48 hours obtains aerobic bacteria mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained aerobic bacteria bacterium solution is by being dried to obtain aerobic microbial inoculum.
3. nano biological circulating water treatment agent according to claim 1, it is characterised in that：The preparation side of the anaerobism microbial inoculum Method, step is as follows,
（1）Anaerobe strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
Anaerobe strain includes methane phase bacillus, methane phase coccus, desulfovibrio, and each strain single culture obtains corresponding Bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Produce first 2% part of alkane bacillus, 3% part of methane phase coccus, 2% part of desulfovibrio；25～30 DEG C of temperature of mixed culture, incubation time 24 ～48 hours, obtain anaerobe mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained anaerobe bacterium solution is by being dried to obtain anaerobism microbial inoculum.
4. nano biological circulating water treatment agent according to claim 1, it is characterised in that：The preparation side of the nitrification microbial inoculum Method, step is as follows,
（1）Nitrifier strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains nitrifier bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, balance of water, Culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained nitrifier bacterium solution is by being dried to obtain nitrification microbial inoculum.
5. nano biological circulating water treatment agent according to claim 1, it is characterised in that：The preparation of the denitrification microbial inoculum Method, step is as follows,
（1）Denitrifying bacterium strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast Cream 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium is through 121 DEG C 20 points Clock high temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains denitrifying bacterium bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, balance of water, Culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained denitrifying bacterium bacterium solution is by being dried to obtain denitrification microbial inoculum.
6. nano biological circulating water treatment agent according to claim 1, it is characterised in that：The preparation side of the polyP bacteria agent Method, step is as follows,
（1）PolyP bacteria strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Yeast extract 1%th, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium through 121 DEG C 20 minutes High temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
（2）Measure above-mentioned bacterium solution to be inoculated in bacterium culture medium, the inoculum concentration of bacterium solution is 5% part by percent by volume；Culture temperature 25～30 DEG C of degree, incubation time 24～48 hours obtains polyP bacteria bacterium solution；
The quality proportioning of bacterium culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, balance of water, Culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained polyP bacteria bacterium solution is by being dried to obtain polyP bacteria agent.
7. nano biological circulating water treatment agent according to claim 1, it is characterised in that：The system of the hydrolysis acidification microbial inoculum Preparation Method, step is as follows,
（1）Hydrolysis acidification bacterium strain is activated in the medium respectively, amplification culture：The quality proportioning of culture medium is：Ferment Female cream 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%、K2HPO40.5%th, balance of water, culture medium is through 121 DEG C 20 Minute high temperature sterilize, cultivation temperature 25-30 DEG C, incubation time 24-48 hours；
Hydrolysis acidification bacterium strain includes Cellumomonas flavigena, starch Clostridium, Bacillus cercuses, succinic acid bacteroid, Each strain single culture, obtains corresponding bacterium solution；
（2）Measure each bacterium solution respectively to be inoculated in mixed bacteria culture medium, the inoculum concentration of each bacterium solution is by percent by volume：Produce yellow 2% part of cellulomonas cartae, 3% part of starch Clostridium, 2% part of Bacillus cercuses, 4% part of succinic acid bacteroid；Mixing 25～30 DEG C of cultivation temperature, incubation time 24～48 hours obtains hydrolysis acidification bacterium mixed bacteria liquid；
The quality proportioning of mixed bacteria culture medium is：Yeast extract 1%, peptone 1.5%, glucose 0.5%, NH4Cl20.5%th, surplus For water, culture medium is through 121 DEG C of 20 minutes high temperature sterilizes；
（3）Gained hydrolysis acidification bacterium bacterium solution is by being dried to obtain hydrolysis acidification microbial inoculum.
8. nano biological circulating water treatment agent according to claim 1, it is characterised in that：Include per 10 parts of growth-promoting enzyme agent 1～2 part of organic carbon source, 1～2 part of organic nitrogen source, 0.5～1 part of compound vitamin, 1～2 part of organic acid remaining as enzyme,
The organic acid selected from citric acid, lactic acid, acetic acid, gluconic acid, malic acid, kojic acid, propanoic acid, succinic acid, ascorbic acid, One or more in salicylic acid；
The enzyme is protease, lipase or cellulase.
9. nano biological circulating water treatment agent according to claim 1, it is characterised in that：
Sepiolite fibre：Meerschaum composition ＞ 85%, granularity：250～300 mesh, 2～2.5g/cm of density3, CaO ＜ 1.5%, Fe2O3 ＜ 0.03%；
Pure silicon algae：SiO2 >=88, true density 2.3g/cm3, the g/cm of bulk density 0.20～0.303, fineness：100～500 mesh；
Volcanic rock charcoal；Particle diameter：1-2mm, density 0.75g/cm3；
Attapulgite：Active substance content >=90%, granularity：100～200 mesh, heap 0.5 ± 1g/cm of density3, water content≤8%；
Zeolite：Clinoptilolite, granularity：180～200 mesh, density 1.92g/cm3, ammonia absorption value ＞ 100mg equivalents/100g, moisture≤ 1.8%。
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