CN105621626A - High-concentration chemical sewage composite inocula and use thereof - Google Patents
High-concentration chemical sewage composite inocula and use thereof Download PDFInfo
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- CN105621626A CN105621626A CN201410619848.2A CN201410619848A CN105621626A CN 105621626 A CN105621626 A CN 105621626A CN 201410619848 A CN201410619848 A CN 201410619848A CN 105621626 A CN105621626 A CN 105621626A
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- 239000010865 sewage Substances 0.000 title claims abstract description 71
- 239000002131 composite material Substances 0.000 title claims abstract description 37
- 239000000126 substance Substances 0.000 title claims abstract description 23
- 241000191938 Micrococcus luteus Species 0.000 claims abstract description 22
- 241000588813 Alcaligenes faecalis Species 0.000 claims abstract description 21
- 241000193752 Bacillus circulans Species 0.000 claims abstract description 21
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 21
- 240000001929 Lactobacillus brevis Species 0.000 claims abstract description 21
- 235000013957 Lactobacillus brevis Nutrition 0.000 claims abstract description 21
- 241000186840 Lactobacillus fermentum Species 0.000 claims abstract description 21
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 21
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 21
- 229940005347 alcaligenes faecalis Drugs 0.000 claims abstract description 21
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 21
- 241000589597 Paracoccus denitrificans Species 0.000 claims abstract description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 17
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 17
- 241000605272 Acidithiobacillus thiooxidans Species 0.000 claims abstract description 5
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 5
- 241000131747 Exiguobacterium acetylicum Species 0.000 claims abstract description 5
- 241000588915 Klebsiella aerogenes Species 0.000 claims abstract description 5
- 229940092559 enterobacter aerogenes Drugs 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 32
- 239000003795 chemical substances by application Substances 0.000 claims description 31
- 150000003839 salts Chemical class 0.000 claims description 24
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 16
- 241000193468 Clostridium perfringens Species 0.000 claims description 16
- 241001468125 Exiguobacterium Species 0.000 claims description 16
- 241000605118 Thiobacillus Species 0.000 claims description 16
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims description 16
- 230000008569 process Effects 0.000 claims description 16
- 241000187694 Rhodococcus fascians Species 0.000 claims description 14
- 241000206596 Halomonas Species 0.000 claims description 13
- 239000012531 culture fluid Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 10
- 230000003203 everyday effect Effects 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 7
- 239000000945 filler Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 229940075612 bacillus cereus Drugs 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 4
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 4
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 4
- 229920002635 polyurethane Polymers 0.000 claims description 4
- 239000004814 polyurethane Substances 0.000 claims description 4
- 235000015099 wheat brans Nutrition 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008485 antagonism Effects 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 239000002068 microbial inoculum Substances 0.000 description 6
- -1 alkyl halide hydro carbons Chemical class 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 239000008176 lyophilized powder Substances 0.000 description 3
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- LQNUZADURLCDLV-UHFFFAOYSA-N nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1 LQNUZADURLCDLV-UHFFFAOYSA-N 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- CMQUQOHNANGDOR-UHFFFAOYSA-N 2,3-dibromo-4-(2,4-dibromo-5-hydroxyphenyl)phenol Chemical class BrC1=C(Br)C(O)=CC=C1C1=CC(O)=C(Br)C=C1Br CMQUQOHNANGDOR-UHFFFAOYSA-N 0.000 description 1
- 241000324499 Alkalibacillus Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000607565 Photobacterium phosphoreum Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- UHOVQNZJYSORNB-UHFFFAOYSA-N benzene Substances C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
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- 230000004151 fermentation Effects 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 208000014451 palmoplantar keratoderma and congenital alopecia 2 Diseases 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.
Description
Technical field
The invention belongs to sewage treatment area, be specifically related to the formula of a kind of high-concentration chemical industry sewage composite bacteria agent capable.
Background technology
The environmental protection industry of China is started late, but quickly grow, all quickly propelling in all kinds of waste water treatment process technology of sewage treatment area and device fabrication at present, all trades and professions have been built up large number of throughout the country, treatment of sewage factory (station) in large scale, achieves good environmental benefit and social benefit. simultaneously, the environmental protection industry of China also faces many Tough questions, due to eager for instant success and quick profits, substantial amounts of manpower and materials flower in process modification in the introducing process of green technology, in the optimization of equipment retrofit and growth of microorganism environment, but the microorganism that biochemical treatment is played a decisive role, unexpectedly few people make inquiries, to such an extent as to AO method, AAO method, AOAO method, SBR method, CASS method, the numerous sewage treatment process of the item such as CAST method is come on stage one after another, patent and the manufacturer of various fillers are too numerous to mention, but still have at present account for significant percentage of sewage treatment plant (station) cannot be properly functioning, particularly in some high-concentration chemical industry sewage process field, manufacturing enterprise spend huge sums build sewage treatment facility, most ruuning situation is not good enough, not only operating cost is high, but also can not qualified discharge, still discharge beyond standards expense is paid to local environmental administration, what have is even forced to stop production.
The trade effluent complicated component of high concentration, including alkyl halide hydro carbons, olefines, alicyclic hydrocarbon type, heteroaromatic hydro carbons, halogeno-benzene alkanes, tetrabromobisphenol class, nitrobenzene, furan, pyridine, the organic pollution of sulfate and various synthetic is along with the waste water of plant emissions or solid waste entered environment, complexity and biological strange property due to these these body structures of material, these pollutant can not be removed by the microorganism of traditional method of wastewater treatment activated sludge culture effectively, these materials accumulate for a long time in the environment, the ecological environment depended on for existence to us causes very big pollution, very big harm is brought to the physical and mental health of the mankind. quite a few industrial pollution enterprise of China would rather be punished also to be unwilling to invest and administer waste water, even if there have sewage-treatment plant to run to be also abnormal.
Therefore, in a kind of unit volume of exploitation, Biomass is many and biofacies enriches, biological activity is high, rapid decomposable organic substance, and the complex microbial inoculum improving COD, BOD, TOC, ammonia nitrogen and certain specific hard-degraded substance clearance is extremely urgent to current sewage disposal technology.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing defect, it is provided that a kind of high-concentration chemical industry sewage composite bacteria agent capable, and this composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Bacillus cercus 180��220, Bacillus circulans 150��160 parts, acetyl Exiguobacterium sp 75��85 parts, Bacillus coagulans 90��120 parts, Pseudomonas aeruginosa 150��170 parts, micrococcus luteus 20��25 parts, thiobacillus thiooxidant 120��140 parts, Rhodococcus fascians 180��230 parts, Lactobacillus brevis 20��30 parts, Paracoccus denitrificans 50��65 parts, alcaligenes faecalis 95��112 parts, pseudomonas putida 110��125 parts, clostridium perfringen 100��130 parts, addicted to salt Halomonas 200��250 parts, beer yeast 280��320 parts, Lactobacillus fermenti 140��160 parts.
Further, this composite bacteria agent capable is mixed by the raw material bacterium of following weight portion: Bacillus cercus 200 parts, Bacillus circulans 155 parts, acetyl Exiguobacterium sp 80 parts, Bacillus coagulans 100 parts, Pseudomonas aeruginosa 160 parts, micrococcus luteus 23 parts, thiobacillus thiooxidant 130 parts, addicted to salt alkali bacillus 200 parts, halo micrococcus luteus 100 parts, photobacterium phosphoreum 30 parts, Lactobacillus brevis 25 parts, Paracoccus denitrificans 60 parts, alcaligenes faecalis 103 parts, pseudomonas putida 120 parts, clostridium perfringen 120 parts, addicted to salt Halomonas 230 parts, beer yeast 300 parts, Lactobacillus fermenti 150 parts.
Strain of the present invention all can from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), China typical culture collection center and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center (ACCC) be commercially available.
The existence form of the composite bacteria agent capable of the present invention is preferably the mixture of lyophilized powder, microbial inoculum or microbial inoculum and solid adjuvant material.
When the existence form of described microbial inoculum is lyophilized powder, preparation method includes Bacillus cercus, Bacillus circulans, acetyl Exiguobacterium sp, Bacillus coagulans, Pseudomonas aeruginosa, micrococcus luteus, Wei Si Salmonella, thiobacillus thiooxidant, Rhodococcus fascians, Lactobacillus brevis, Paracoccus denitrificans, alcaligenes faecalis, pseudomonas putida, clostridium perfringen, beer yeast, Lactobacillus fermenti prepare fermentation liquid respectively, after mixing according to mass ratio, make lyophilized powder by this area conventional method.
A kind of method that high-concentration chemical industry sewage composite bacteria agent capable utilizing the present invention processes sewage, it is characterised in that include following step:
1), polyurethane block filler that diameter the is 0.5��3cm carrier as composite bacteria agent capable is being laid at the bottom of the pond of sewage reactor;
2), adding the 5LCOD diluted treatment sewage at 800��1000mg/L in sewage reactor, add glucose 150��160g, dipotassium hydrogen phosphate 6��8g, Magnesium sulfate heptahydrate 6��8g, wheat bran 40��60g, stirring and dissolving makes culture fluid; The pH of culture fluid is adjusted to 7��7.5;
3), in culture fluid, composite bacteria agent capable 50��80g is added; It is uniformly mixed;
4), water temperature control, at 30��35 DEG C, to stir every day 3��5 times, ventilative half an hour every time;
5), the clearance of the COD of sewage, ammonia nitrogen etc. in every day assaying reaction device; Guarantee that COD of sewage is reducing day by day, represent that strain starts growth, after COD of sewage drops to below 300mg/L, step up concentration and the salinity of influent COD, but total salt concentration need to be controlled and be not more than 40000mg/L, when after 5��10 days circulate, start to continue through the treatment sewage of not diluted.
The invention has the beneficial effects as follows: the sewage disposal composite flora of the present invention can adopt a dosing method, easy and simple to handle, is beneficial to production, it is possible to quickly form dominant microflora; Reasonable compatibility between each strain in formula, symbiosis is coordinated, mutually not antagonism, and the survival rate of microbial inoculum is high. The method that the microbial inoculum using this formula processes sewage is simple, macromole, difficult degradation, poisonous and harmful substance such as phenols, arene and ammonia nitrogen, phosphorus, high-sulfate etc. are had the treatment effect of uniqueness, COD clearance may be up to 94.17%, there is significantly high Treatment Stability and utilization rate, be suitable to high-concentration chemical industry sewage process, the process water yield and water quality treatment can be improved, reduce operating cost, promote qualified discharge.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is merely to illustrate and explains the present invention, is not intended to limit the present invention.
Embodiment 1
One utilizes high-concentration chemical industry sewage composite bacteria agent capable, and this composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Bacillus cercus 200 parts, Bacillus circulans 155 parts, acetyl Exiguobacterium sp 80 parts, Bacillus coagulans 100 parts, Pseudomonas aeruginosa 160 parts, micrococcus luteus 23 parts, thiobacillus thiooxidant 130 parts, Lactobacillus brevis 25 parts, alcaligenes faecalis 103 parts, pseudomonas putida 120 parts, clostridium perfringen 120 parts, addicted to salt Halomonas 230 parts, beer yeast 300 parts, Lactobacillus fermenti 150 parts.
Wherein said Bacillus cercus is Bacillus cercus (Bacilluscereus): CGMCCNO.3836;
Bacillus circulans is Bacillus circulans is (Bacilluscirculans): CGMCC1.554;
Acetyl Exiguobacterium sp is acetyl Exiguobacterium sp (Exiguobacteriumacetylicum): CGMCCNo.4137;
Bacillus coagulans is Bacillus coagulans is (Bacilluscoagulans) CGMCCNo.7646;
Pseudomonas aeruginosa is Pseudomonas aeruginosa (Pseudomonasaeruginosa): ATCC15442;
Micrococcus luteus is micrococcus luteus (Micrococcusluteus): CGMCCNo.6072;
Thiobacillus thiooxidant is thiobacillus thiooxidant (Thiobacillusthiooxidans) CGMCCNO.2158;
Rhodococcus fascians is Rhodococcus fascians (Rhodococcussp.) CGMCCNo.5287;
Lactobacillus brevis is Lactobacillus brevis (Lactobacillusbrevis) CGMCCNo.6683;
Paracoccus denitrificans is Paracoccus denitrificans (Paracoccusdenitrificans) ACCC10489;
Alcaligenes faecalis is alcaligenes faecalis (Alcaligenesfaecalis) CCTCCNO:M2013176;
Pseudomonas putida is pseudomonas putida (Pseudomonasputida) CCTCCNO:M2013457;
Clostridium perfringen is clostridium perfringen (Enterobacteraerogenes) CGMCC1969;
It is addicted to salt Halomonas (Halomonashalophile) CGMCC12316 addicted to salt Halomonas;
Beer yeast is beer yeast (Saccharomycescerevisiac) ACCC20203;
Lactobacillus fermenti is Lactobacillus fermenti is (Lactobacillusfermentum) CGMCCNo.4648.
Embodiment 2
One utilizes high-concentration chemical industry sewage composite bacteria agent capable, and this composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Bacillus cercus 180 parts, Bacillus circulans 150 parts, acetyl Exiguobacterium sp 75 parts, Bacillus coagulans 90 parts, Pseudomonas aeruginosa 150 parts, micrococcus luteus 20 parts, thiobacillus thiooxidant 120 parts, Rhodococcus fascians 180 parts, Lactobacillus brevis 20 parts, Paracoccus denitrificans 50 parts, alcaligenes faecalis 95 parts, pseudomonas putida 110 parts, clostridium perfringen 100 parts, beer yeast 280 parts, Lactobacillus fermenti 140 parts;
Wherein said Bacillus cercus is Bacillus cercus (Bacilluscereus): CGMCCNo.6074;
Bacillus circulans is Bacillus circulans is (Bacilluscirculans): CGMCC1.383;
Acetyl Exiguobacterium sp is acetyl Exiguobacterium sp (Exiguobacteriumacetylicum): CGMCCNo.4137;
Bacillus coagulans is Bacillus coagulans (Bacilluscoagulans) CCTCCM206012
Pseudomonas aeruginosa is Pseudomonas aeruginosa (Pseudomonasaeruginosa): CGMCCNo.1.239;
Micrococcus luteus is micrococcus luteus (Micrococcusluteus): CGMCCNo.7584;
Thiobacillus thiooxidant is thiobacillus thiooxidant (Thiobacillusthiooxidans): CGMCCNO.0759;
Rhodococcus fascians is Rhodococcus fascians (Rhodococcussp.): CGMCCNo.4762;
Lactobacillus brevis is Lactobacillus brevis (Lactobacillusbrevis): CGMCCNO.1306;
Paracoccus denitrificans is Paracoccus denitrificans (Paracoccusdenitrificans): CGMCCNo.3658;
Alcaligenes faecalis is alcaligenes faecalis (Alcaligenesfaecalis): CCTCCNo.M205095;
Pseudomonas putida is pseudomonas putida (Pseudomonasputida): CCTCCNO:2010342;
Clostridium perfringen is clostridium perfringen (Enterobacteraerogenes) CGMCCNo.4155;
It is addicted to salt Halomonas (Halomonashalophile) CGMCC12316 addicted to salt Halomonas;
Beer yeast is beer yeast (Saccharomycescerevisiac): ACCC20203
Lactobacillus fermenti is Lactobacillus fermenti (Lactobacillusfermentum): CGMCCNo.0843.
Embodiment 3
One utilizes high-concentration chemical industry sewage composite bacteria agent capable, and this composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Bacillus cercus 220 parts, Bacillus circulans 160 parts, acetyl Exiguobacterium sp 85 parts, Bacillus coagulans 120 parts, Pseudomonas aeruginosa 170 parts, micrococcus luteus 25 parts, thiobacillus thiooxidant 140 parts, Rhodococcus fascians 230 parts, Lactobacillus brevis 30 parts, Paracoccus denitrificans 65 parts, alcaligenes faecalis 112 parts, pseudomonas putida 125 parts, clostridium perfringen 130 parts, beer yeast 320 parts, Lactobacillus fermenti 160 parts.
Wherein said Bacillus cercus is Bacillus cercus (Bacilluscereus): CGMCCNo.6074;
Bacillus circulans is Bacillus circulans is (Bacilluscirculans): ATCC13403;
Acetyl Exiguobacterium sp acetyl Exiguobacterium sp is (Exiguobacteriumacetylicum): CGMCCNo.4137;
Bacillus coagulans is Bacillus coagulans (Bacilluscoagulans) CGMCCNo.7646;
Pseudomonas aeruginosa is Pseudomonas aeruginosa (Pseudomonasaeruginosa): ACCC02722;
Micrococcus luteus is micrococcus luteus (Micrococcusluteus): CGMCCNo.7584;
Thiobacillus thiooxidant is thiobacillus thiooxidant (Thiobacillusthiooxidans) CGMCCNO.2158;
Rhodococcus fascians is Rhodococcus fascians (Rhodococcussp.) CGMCCNo.4762;
Lactobacillus brevis is Lactobacillus brevis (Lactobacillusbrevis) CGMCCNO.1306;
Paracoccus denitrificans is Paracoccus denitrificans (Paracoccusdenitrificans) ACCC10489;
Alcaligenes faecalis is alcaligenes faecalis (Alcaligenesfaecalis) CCTCCNo.M205095
Pseudomonas putida is pseudomonas putida (Pseudomonasputida): CCTCCNO:M209146;
Clostridium perfringen is clostridium perfringen: (Enterobacteraerogenes) CGMCCNo.3163;
It is addicted to salt Halomonas (Halomonashalophile) CGMCC12316 addicted to salt Halomonas;
Beer yeast is beer yeast (Saccharomycescerevisiac) ACCC20203;
Lactobacillus fermenti is Lactobacillus fermenti (Lactobacillusfermentum) CGMCCNo.4648.
Embodiment 4
The formula flora of a kind of composite bacteria agent capable utilized in embodiments of the invention 1 processes the method for sewage:
1) it is, the 0.5 polyurethane block filler carrier as composite bacteria agent capable laying diameter at the bottom of the pond of sewage reactor;
2), adding the 5LCOD diluted treatment sewage at 800g/L, total salt concentration about 4000mg/L in sewage reactor, add glucose 150g, dipotassium hydrogen phosphate 6g, Magnesium sulfate heptahydrate 6g, wheat bran 60g, stirring and dissolving makes culture fluid; The pH of culture fluid is adjusted to about 7;
3), in culture fluid, composite bacteria agent capable 50g is added; It is uniformly mixed;
4), water temperature control, at 35 DEG C, to stir every day 3��5 times, ventilative half an hour every time;
5), the clearance of the COD of sewage, ammonia nitrogen etc. in every day assaying reaction device; Guarantee that COD of sewage is reducing day by day, represent that strain starts growth, after COD of sewage drops to below 300mg/L, passing into COD and be diluted in 1500g/L, total salt concentration is diluted in the treatment sewage of about 7500mg/L, after COD of sewage drops to below 300mg/L, pass into COD and be diluted in 3500g/L, total salt concentration is diluted in the treatment sewage of about 18000mg/L, starts, at full capacity by continuous print sewage, to be no longer diluted after COD of sewage drops to below 300mg/L. After the method for the present embodiment processes 2 weeks, the clearance of the COD of water outlet is up to 91.8%, and the clearance of ammonia nitrogen is up to 97.5%, and total salt concentration also has the clearance of 9.6% simultaneously, and result is as shown in table 1.
The high-concentration chemical industry sewage composite bacteria agent capable treatment effect statistics of table 1 present invention
| Project name | Water quality before treatment | Water quality after process | Clearance |
| COD(mg/L) | 5500 | 451 | 91.8% |
| Ammonia nitrogen (mg/L) | 650 | 16 | 97.5% |
| Salinity (mg/L) | 25000 | 22600 | 9.6% |
Embodiment 5
The formula flora of a kind of composite bacteria agent capable utilized in embodiments of the invention 3 processes the method for sewage:
1), polyurethane block filler that diameter the is 3cm carrier as composite bacteria agent capable is being laid at the bottom of the pond of sewage reactor;
2), adding 5LCOD in sewage reactor at 1000mg/L, total salt concentration is the diluted treatment sewage of about 3500mg/L, adds glucose 160g, dipotassium hydrogen phosphate 8g, Magnesium sulfate heptahydrate 8g, wheat bran 60g, and stirring and dissolving makes culture fluid; The pH of culture fluid is adjusted to about 7.5;
3), in culture fluid, composite bacteria agent capable 80g is added; It is uniformly mixed;
4), water temperature control, at 32��35 DEG C, to stir every day 3��5 times, ventilative half an hour every time;
5), the clearance of the COD of sewage, ammonia nitrogen etc. in every day assaying reaction device; Guarantee that COD of sewage is reducing day by day, represent that strain starts growth, after COD of sewage drops to below 300mg/L, passing into COD and be diluted in 2000g/L, total salt concentration is diluted in the treatment sewage of about 7000mg/L, after COD of sewage drops to below 300mg/L, pass into COD and be diluted in 5000g/L's, total salt concentration is diluted in the treatment sewage of 19000mg/L, starts, at full capacity by continuous print sewage, to be no longer diluted after COD of sewage drops to below 300mg/L. After the method for the present embodiment processes 2 weeks, the clearance of the COD of water outlet is up to 92.8%, and the clearance of ammonia nitrogen is up to 95.7%, and total salt also has the clearance of 8.7% simultaneously, and concrete outcome is as shown in table 2.
The high-concentration chemical industry sewage composite bacteria agent capable treatment effect statistics of table 2 present invention
| Project name | Water quality before treatment | Water quality after process | Clearance |
| COD(mg/L) | 6200 | 446 | 92.8% |
| Ammonia nitrogen (mg/L) | 539 | 23 | 95.7% |
| Salinity (mg/L) | 23000 | 21000 | 8.7% |
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement. All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (4)
1. a high-concentration chemical industry sewage composite bacteria agent capable, this composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Bacillus cercus 180��220 parts, Bacillus circulans 150��160 parts, acetyl Exiguobacterium sp 75��85 parts, Bacillus coagulans 90��120 parts, Pseudomonas aeruginosa 150��170 parts, micrococcus luteus 20��25 parts, thiobacillus thiooxidant 120��140 parts, Rhodococcus fascians 180��230 parts, Lactobacillus brevis 20��30 parts, Paracoccus denitrificans 50��65 parts, alcaligenes faecalis 95��112 parts, pseudomonas putida 110��125 parts, clostridium perfringen 100��130 parts, addicted to salt Halomonas 200��250 parts, beer yeast 280��320 parts, Lactobacillus fermenti 140��160 parts.
2. a kind of high-concentration chemical industry sewage composite bacteria agent capable as claimed in claim 1, it is characterized in that, described composite bacteria agent capable is mixed by the raw material bacterium of following weight portion: Bacillus cercus 200 parts, Bacillus circulans 155 parts, acetyl Exiguobacterium sp 80 parts, Bacillus coagulans 100 parts, Pseudomonas aeruginosa 160 parts, micrococcus luteus 23 parts, thiobacillus thiooxidant 130 parts, Rhodococcus fascians 200 parts, Lactobacillus brevis 25 parts, Paracoccus denitrificans 60 parts, alcaligenes faecalis 103 parts, pseudomonas putida 120 parts, clostridium perfringen 120 parts, addicted to salt Halomonas 230 parts, beer yeast 300 parts, Lactobacillus fermenti 150 parts.
3. a kind of high-concentration chemical industry sewage composite bacteria agent capable as claimed in claim 2, it is characterised in that wherein said Bacillus cercus is Bacillus cercus (Bacilluscereus): CGMCCNO.3836;
Bacillus circulans is Bacillus circulans is (Bacilluscirculans): CGMCC1.554;
Acetyl Exiguobacterium sp is acetyl Exiguobacterium sp (Exiguobacteriumacetylicum): CGMCCNo.4137;
Bacillus coagulans is Bacillus coagulans is (Bacilluscoagulans) CGMCCNo.7646;
Pseudomonas aeruginosa is Pseudomonas aeruginosa (Pseudomonasaeruginosa): ATCC15442;
Micrococcus luteus is micrococcus luteus (Micrococcusluteus): CGMCCNo.6072;
Thiobacillus thiooxidant is thiobacillus thiooxidant (Thiobacillusthiooxidans) CGMCCNO.2158;
Rhodococcus fascians is Rhodococcus fascians (Rhodococcussp.) CGMCCNo.5287;
Lactobacillus brevis is Lactobacillus brevis (Lactobacillusbrevis) CGMCCNo.6683;
Paracoccus denitrificans is Paracoccus denitrificans (Paracoccusdenitrificans) ACCC10489;
Alcaligenes faecalis is alcaligenes faecalis (Alcaligenesfaecalis) CCTCCNO:M2013176;
Pseudomonas putida is pseudomonas putida (Pseudomonasputida) CCTCCNO:M2013457;
Clostridium perfringen is clostridium perfringen (Enterobacteraerogenes) CGMCC1969;
It is addicted to salt Halomonas (Halomonashalophile) CGMCC12316 addicted to salt Halomonas;
Beer yeast is beer yeast (Saccharomycescerevisiac) ACCC20203;
Lactobacillus fermenti is Lactobacillus fermenti is (Lactobacillusfermentum) CGMCCNo.4648.
4. the method that the high-concentration chemical industry sewage composite bacteria agent capable described in any one of claim 1-3 utilizing the present invention processes sewage, it is characterised in that include following step:
1), polyurethane block filler that diameter the is 0.5��3cm carrier as composite bacteria agent capable is being laid at the bottom of the pond of sewage reactor;
2), adding the 5LCOD diluted treatment sewage at 800��1000mg/L in sewage reactor, add glucose 150��160g, dipotassium hydrogen phosphate 6��8g, Magnesium sulfate heptahydrate 6��8g, wheat bran 40��60g, stirring and dissolving makes culture fluid; The pH of culture fluid is adjusted to 7��7.5;
3), in culture fluid, composite bacteria agent capable 50��80g is added; It is uniformly mixed;
4), water temperature control, at 30��35 DEG C, to stir every day 3��5 times, ventilative half an hour every time;
5), the clearance of the COD of sewage, ammonia nitrogen etc. in every day assaying reaction device; Guarantee that COD of sewage is reducing day by day, represent that strain starts growth, after COD of sewage drops to below 300mg/L, step up concentration and the salinity of influent COD, but the total salt concentration that need to control sewage is not more than 40000mg/L, when after 5��10 days circulate, start to continue through the treatment sewage of not diluted.
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