CN108611253A - A kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device and breeding method - Google Patents

A kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device and breeding method Download PDF

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Publication number
CN108611253A
CN108611253A CN201810414311.0A CN201810414311A CN108611253A CN 108611253 A CN108611253 A CN 108611253A CN 201810414311 A CN201810414311 A CN 201810414311A CN 108611253 A CN108611253 A CN 108611253A
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activated sludge
cultivation reactor
cultivation
black liquor
salt tolerant
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邓文海
冯睿
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Tianjin E Tech Energy Technology Development Co Ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention discloses a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge to prepare storage device and its breeding method, including:Cultivation reactor, the cultivation reactor include strain filling line, activated sludge filling line, nutritive salt filling line, fresh water filling pipe road and compressed air filling line;On the outside of the cultivation reactor, it is equipped with plate and frame filter press;The pneumatic diaphragm slush pump of conveying mud is equipped between the cultivation reactor and the plate and frame filter press;The y-type filter of filtering overburden is equipped between the valve C and the pneumatic diaphragm slush pump;Check-valves is equipped between the pneumatic diaphragm slush pump and the plate and frame filter press;It is equipped with filtrate return pipeline between the plate and frame filter press and the cultivation reactor, storage mud bucket is equipped with below the plate and frame filter press;The cultivation reactor is equipped with the exhaust pipe with tarpaulin.Cultivation operation can be rapidly completed in production platform in the present invention, carry out sanitary sewage disposal.

Description

A kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device and breeding method
Technical field
The present invention relates to field of environment engineering technology, and storage dress is prepared more particularly to a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge It sets and breeding method.
Background technology
Production platform mostly uses greatly the processing that biochemical process carries out sanitary sewage at present, is initially thrown in sewage treatment unit It is required for carrying out the cultivation domestication work of activated sludge when fortune, just can guarantee the normal operation of processing unit.But it is light in order to save Water, production platform more at present all use seawater toilet-flushing, this results in sanitary sewage salt content higher, and then makes sewage disposal It is more difficult that activated sludge when device initially puts into operation cultivates work.
Under the conditions of current activated sludge Cultivating techniques, the activated sludge that Efficient salt-tolerant is tamed out in culture needs 20-30 days, The domestication time of more normal activated sludge is 1-2 times long, and is drained after in-situ processing often not up to standard.
Invention content
The present invention is directed to above-mentioned technical problem of the existing technology, and providing one kind can be rapidly completed in production platform The salt tolerant Black Liquor with Efficient Bacteria activated sludge for cultivating operation prepares storage device and breeding method.
The present invention is adopted the technical scheme that solve technical problem present in known technology:
A kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device, including:Cultivation reactor, the cultivation reactor include Strain filling line, sludge filling line, nutritive salt filling line, fresh water filling pipe road and compressed air filling line living;Institute It states and is equipped with valve A between fresh water filling pipe road and the cultivation reactor;The compressed air filling line and the cultivation are anti- It answers and is equipped with valve B between device;
On the outside of the cultivation reactor, it is equipped with plate and frame filter press;The cultivation reactor and the plate and frame filter press it Between be equipped with conveying mud pneumatic diaphragm slush pump;Control is equipped between the pneumatic diaphragm slush pump and the cultivation reactor The valve C of mud speed rate processed;The Y type mistakes of filtering overburden are equipped between the valve C and the pneumatic diaphragm slush pump Filter;Check-valves is equipped between the pneumatic diaphragm slush pump and the plate and frame filter press;The check-valves and the sheet frame pressure Pressure gauge and valve D are equipped between filter;Filtrate recurrent canal is equipped between the plate and frame filter press and the cultivation reactor Road, the plate and frame filter press lower section are equipped with storage mud bucket;The cultivation reactor is equipped with the exhaust pipe with tarpaulin.
Further, a method of it preparing storage device using the above salt tolerant Black Liquor with Efficient Bacteria activated sludge and is cultivated, including Following steps:
Step 1 cultivates goods and materials preparation:
(1):100N L salt tolerant high-efficiency strains are added into the cultivation reactor by the strain filling line;
(2):100N L activated sludge is added into cultivation reactor by the activated sludge filling line;
(3):The compounding nutritive salt of 10N kg is added into cultivation reactor by the nutritive salt filling line;
(4):By adding N m in the fresh water filling pipe road direction cultivation reactor3Fresh water;
(5):It opens valve B and allows compressed air into cultivation reactor, exposed by every interruption for stopping 1 hour in 6 hours that is aerated Gas maintains the content of dissolved oxygen in cultivation reactor in 6-10mg/L;
Step (5) continues to cultivation operation to terminate;
The salt tolerant high-efficiency strain includes:Candida lipolytica bacterium 20%, saccharomyces ellipsoideus bacterium 20%, stearothermophilus gemma bar Bacterium 20%, pseudomonad class 20%, denitrogenation bacillus etc. microorganism 20%;
The activated sludge is that life sludge of sewage treatment plant pond is press-filtered out the activated sludge filtrate come, the activated sludge Aqueous 90% and be free of flocculant;
The compounding nutritive salt uses food-grade glucose and ammonium dihydrogen phosphate, by C:N:P=100:5:1 ratio is matched System;
The fresh water is tap water;
Step 2 cultivates operation:
(1):Daily detection COD in morning contents, dissolved oxygen content, pH value, temperature and activated sludge settling ratio SV30;According to COD situations are detected, addition compounding nutritive salt maintains cultivation reactor COD contents in 9.5-10.5g/L;Make PH in cultivation reactor It it is 15-30 DEG C for 6-9, temperature;The content of dissolved oxygen maintains 6-10mg/L in cultivation reactor;
Step (1) is repeated until SV30 reaches 70% or more;
(2):NaCl is added in three times into cultivation reactor by nutritive salt filling line:
When SV30 reaches 75%, adding a NaCl makes the goods and materials salinity in cultivation reactor reach 0.5%;Three days with Afterwards, when SV30 remains as 75%, then a NaCl is added, the goods and materials salinity in cultivation reactor is made to reach 1.0%;Three days with Afterwards, when SV30 remains as 75%, NaCl is added for the last time, the goods and materials salinity in cultivation reactor is made to reach 1.5%;
(3):After three days, when SV30 remains as 75%, 85% salt tolerant Black Liquor with Efficient Bacteria activated sludge is filtered out, is dropped using SV30 As low as 10%.
(4):It is press-filtered out salt tolerant Black Liquor with Efficient Bacteria activated sludge using the plate and frame filter press;The salt tolerant Black Liquor with Efficient Bacteria come is press-filtered out to live Property sewage sludge storage is in special storage mud bucket;Obtained filtrate returns to cultivation reactor by the filtrate return pipeline, continues The operation of step (1) to (3), until being press-filtered out 200N L salt tolerant Black Liquor with Efficient Bacteria activated sludge.
(5):Compounding nutritive salt is added in the salt tolerant Black Liquor with Efficient Bacteria activated sludge that step (4) obtains, keeps the salt tolerant efficient The ratio of bacterium activated sludge and compounding nutritive salt is 20:1, stand for standby use after addition.
Further, PH is 6 in cultivation reactor in step 2, temperature is 22 DEG C;The content of dissolved oxygen is in cultivation reactor 6mg/L;COD is 9.8g/L.
Further, the N=1.
Further, the volume of the cultivation reactor is 200L;500 type of plate and frame filter press model, volume are 1m3;The volume of the storage mud tank is 200L.
Further, the CFU/g10 of salt tolerant high-efficiency strain described in step 1 is 5.0x109.
The invention has the advantages and positive effects that:
A kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge of the present invention prepares storage device and breeding method, by cultivation reactor In add cultivation goods and materials, through cultivation operate, be satisfied the activated sludge of sewage disposal requirement.The salt tolerant of the present invention is efficient Bacterium activated sludge prepares storage device and breeding method through production platform field practice, has smoothly completed live sanitary sewage disposal The cultivation work of device, sewage treatment unit drain normal, stably reaching standard;Improve current activated sludge Cultivating techniques Under the conditions of, the activated sludge acclimatization time is long, drains problem not up to standard.
Description of the drawings
Attached drawing 1 is the structure chart of the preferred embodiment of the present invention;
Wherein, 1, cultivation reactor;1-1, strain filling line;1-2, activated sludge filling line;1-3, nutritive salt note Enter pipeline;1-4, fresh water filling pipe road;1-5, compressed air filling line;2, plate and frame filter press;3, mud bucket is stored up;4, valve A;5、 Valve B;6, pneumatic diaphragm slush pump;7, valve C;8, y-type filter;9, check-valves;10, pressure gauge;11, valve D;12, it filters Liquid return pipeline;13, exhaust pipe.
Specific implementation mode
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and coordinate attached drawing Detailed description are as follows:
Referring to Fig. 1, a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device, including:Cultivation reactor 1, the training Bacterium reactor 1 includes strain filling line 1-1, activated sludge filling line 1-2, nutritive salt filling line 1-3, fresh water filling pipe Road 1-4 and compressed air filling line 1-5;Preferably, it is set between the fresh water filling pipe road 1-4 and the cultivation reactor 1 There is valve A4;Likewise, being equipped with valve B5 between the compressed air filling line 1-5 and the cultivation reactor 1;
In 1 outside of the cultivation reactor, it is equipped with plate and frame filter press 2;The cultivation reactor 1 and the plate and frame filter press The pneumatic diaphragm slush pump 6 of conveying mud is equipped between 2;Between the pneumatic diaphragm slush pump 6 and the cultivation reactor 1 Valve C7 equipped with control mud speed rate;It is equipped between the valve C7 and the pneumatic diaphragm slush pump 6 miscellaneous in filtering mud The y-type filter 8 of matter;Check-valves 9 is equipped between the pneumatic diaphragm slush pump 6 and the plate and frame filter press 2;The check-valves Pressure gauge 10 and valve D11 are equipped between 9 and the plate and frame filter press 2;In the plate and frame filter press 2 and the cultivation reactor Filtrate return pipeline 12 is equipped between 1,2 lower section of the plate and frame filter press is equipped with storage mud bucket 3;The cultivation reactor 1 is equipped with Exhaust pipe 13 with tarpaulin.
Embodiment
The present embodiment to cultivate using above-mentioned salt tolerant Black Liquor with Efficient Bacteria activated sludge prepare storage device cultivate 200L salt tolerants it is efficient Bacterium activated sludge, uses following methods and step:
The equipment of use:
The volume of the cultivation reactor 1 is 200L;
2 model of the plate and frame filter press, 500 type, volume 1m3
The volume of the storage mud bucket 3 is 200L;
Step 1 cultivates goods and materials preparation:
(1) 100NL salt tolerant high-efficiency strains are added into the cultivation reactor 1 by the strain filling line 1-1;
(2) 100NL activated sludge is added into cultivation reactor 1 by the activated sludge filling line 1-2;
(3) the compounding nutritive salt of 10Nkg is added into cultivation reactor 1 by the nutritive salt filling line 1-3;
(4) Nm is added into cultivation reactor 1 by the fresh water filling pipe road 1-43Fresh water;
(5) it opens valve B5 and allows compressed air into cultivation reactor 1, maintained in cultivation reactor 1 by being interrupted to be aerated The content of dissolved oxygen is in 6-10mg/L;Stop 1 hour specifically, being often aerated 6 hours;
Above step (1) to step (4) adds the sequence of cultivation goods and materials in no particular order;
Step (5) continues to cultivation operation to terminate;
Preferably, the salt tolerant high-efficiency strain includes:Candida lipolytica bacterium 20%, saccharomyces ellipsoideus bacterium 20%, thermophilic fat Fat bacillus 20%, pseudomonad class 20%, denitrogenation bacillus etc. microorganism 20%;Preferably, CFU/g10 about 5.0x109 It is a;
Preferably, the activated sludge is that life sludge of sewage treatment plant pond is press-filtered out the activated sludge filtrate come, described Activated sludge aqueous 90% and be free of flocculant;
Preferably, the compounding nutritive salt uses food-grade glucose and ammonium dihydrogen phosphate, by C:N:P=100:5:1 Ratio is prepared;
Preferably, the fresh water is tap water;
Step 2 cultivates operation:
(1) daily detection COD in morning contents, dissolved oxygen content, pH value, temperature and activated sludge settling ratio SV30;According to COD situations are detected, addition compounding nutritive salt maintains cultivation reactor 1COD contents in 10g/L or so;
Preferably, PH is 6-9 in cultivation reactor 1, temperature is 15-30 DEG C;The content dimension of dissolved oxygen in cultivation reactor 1 It holds in 6-10mg/L;COD is that 9.5-10.5g/L grows conducive to salt tolerant high-efficiency strain;
Preferably, PH is 6 in cultivation reactor 1, temperature is 22 DEG C;The content of dissolved oxygen is 6mg/ in cultivation reactor 1 L;COD is 9.8g/L.
Step (1) is repeated until SV30 reaches 70% or more;
(2) NaCl is added by nutritive salt filling line 1-3 in three times into cultivation reactor 1:
When SV30 reaches 75%, adding a NaCl makes the goods and materials salinity in cultivation reactor 1 reach 0.5%;Three days
After, when SV30 remains as 75%, then a NaCl is added, the goods and materials salinity in cultivation reactor 1 is made to reach 1.0%;
After three days, when SV30 remains as 75%, NaCl is added for the last time, makes the goods and materials salinity in cultivation reactor 1
Reach 1.5%;
After (3) three days, when SV30 remains as 75%, 85% salt tolerant Black Liquor with Efficient Bacteria activated sludge is filtered out, is dropped using SV30 As low as 10%.
(4) it is press-filtered out salt tolerant Black Liquor with Efficient Bacteria activated sludge using the plate and frame filter press 2;The salt tolerant Black Liquor with Efficient Bacteria come is press-filtered out to live Property sewage sludge storage in storage mud bucket 3;Obtained filtrate returns to cultivation reactor 1 by the filtrate return pipeline 12, continues The operation of step (1) to (3), until being press-filtered out 200L salt tolerant Black Liquor with Efficient Bacteria activated sludge.
(5) compounding nutritive salt is added in the salt tolerant Black Liquor with Efficient Bacteria activated sludge that step (4) obtains, makes the salt tolerant Black Liquor with Efficient Bacteria The ratio of activated sludge and compounding nutritive salt is 20:1, stand for standby use after addition.
The salt tolerant Black Liquor with Efficient Bacteria activated sludge of the present invention prepares storage device and breeding method through production platform field practice, suitable Profit completes the cultivation work of live sewage treatment unit, and sewage treatment unit drains normal, stably reaching standard;And this The activated sludge acclimatization time of invention, which has, to be obviously shortened.
The embodiments of the present invention have been described in detail above, but content is only the preferred embodiment of the present invention, It should not be construed as limiting the practical range of the present invention.Any changes and modifications in accordance with the scope of the present application, It should all still fall within the scope of the patent of the present invention.

Claims (6)

1. a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device, including:Cultivation reactor (1), the cultivation reactor (1) Including strain filling line (1-1), activated sludge filling line (1-2), nutritive salt filling line (1-3), fresh water filling pipe road (1-4) and compressed air filling line (1-5);
It is characterized in that:Valve A (4) is equipped between the fresh water filling pipe road (1-4) and the cultivation reactor (1);It is described Valve B (5) is equipped between compressed air filling line (1-5) and the cultivation reactor (1);
On the outside of the cultivation reactor (1), it is equipped with plate and frame filter press (2);The cultivation reactor (1) and the plate compression The pneumatic diaphragm slush pump (6) of conveying mud is equipped between machine (2);The pneumatic diaphragm slush pump (6) and the cultivation are anti- Answer the valve C (7) that control mud speed rate is equipped between device (1);Between the valve C (7) and the pneumatic diaphragm slush pump (6) Y-type filter (8) equipped with filtering overburden;Between the pneumatic diaphragm slush pump (6) and the plate and frame filter press (2) Equipped with check-valves (9);Pressure gauge (10) and valve D (11) are equipped between the check-valves (9) and the plate and frame filter press (2); Filtrate return pipeline (12), the plate and frame filter press are equipped between the plate and frame filter press (2) and the cultivation reactor (1) (2) lower section is equipped with storage mud bucket (3);The cultivation reactor (1) is equipped with the exhaust pipe (13) with tarpaulin.
2. a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge using described in claim 1 prepares the method that storage device is cultivated, It is characterized in that:Include the following steps:
Step 1 cultivates goods and materials preparation:
(1) 100N L salt tolerant high-efficiency strains are added into the cultivation reactor (1) by the strain filling line (1-1);
(2) 100N L activated sludge is added into cultivation reactor (1) by the activated sludge filling line (1-2);
(3) the compounding nutritive salt of 10N kg is added into cultivation reactor (1) by the nutritive salt filling line (1-3);
(4) N m are added into cultivation reactor (1) by the fresh water filling pipe road (1-4)3Fresh water;
(5) it opens valve B (5) and allows compressed air into cultivation reactor (1), exposed by every interruption for stopping 1 hour in 6 hours that is aerated Gas maintains the content of cultivation reactor (1) interior dissolved oxygen in 6-10mg/L;
Step (5) continues to cultivation operation to terminate;
The salt tolerant high-efficiency strain includes:Candida lipolytica bacterium 20%, saccharomyces ellipsoideus bacterium 20%, bacillus stearothermophilus 20%, pseudomonad class 20%, denitrogenation bacillus etc. microorganism 20%;
The activated sludge is that life sludge of sewage treatment plant pond is press-filtered out the activated sludge filtrate come, and the activated sludge is aqueous 90% and be free of flocculant;
The compounding nutritive salt uses food-grade glucose and ammonium dihydrogen phosphate, by C:N:P=100:5:1 ratio is prepared;
The fresh water is tap water;
Step 2 cultivates operation:
(1) daily detection COD in morning contents, dissolved oxygen content, pH value, temperature and activated sludge settling ratio SV30;According to detection COD situations, addition compounding nutritive salt maintain cultivation reactor (1) COD contents in 9.5-10.5g/L;Make in cultivation reactor (1) PH is 6-9, temperature is 15-30 DEG C;The content of cultivation reactor (1) interior dissolved oxygen maintains 6-10mg/L;
Step (1) is repeated until SV30 reaches 70% or more;
(2) NaCl is added by nutritive salt filling line (1-3) in three times into cultivation reactor (1):
When SV30 reaches 75%, adding a NaCl makes the goods and materials salinity in cultivation reactor (1) reach 0.5%;Three days with Afterwards, when SV30 remains as 75%, then a NaCl is added, the goods and materials salinity in cultivation reactor (1) is made to reach 1.0%;Three days After, when SV30 remains as 75%, NaCl is added for the last time, the goods and materials salinity in cultivation reactor (1) is made to reach 1.5%;
After (3) three days, when SV30 remains as 75%, 85% salt tolerant Black Liquor with Efficient Bacteria activated sludge is filtered out, is reduced to using SV30 10%.
(4) it is press-filtered out salt tolerant Black Liquor with Efficient Bacteria activated sludge using the plate and frame filter press (2);It is press-filtered out the salt tolerant Black Liquor with Efficient Bacteria activity come Sewage sludge storage is in the storage mud bucket (3);Obtained filtrate returns to cultivation reactor (1) by the filtrate return pipeline (12), Continue the operation of step (1) to (3), until being press-filtered out 200N L salt tolerant Black Liquor with Efficient Bacteria activated sludge.
(5) compounding nutritive salt is added in the salt tolerant Black Liquor with Efficient Bacteria activated sludge that step (4) obtains, makes the salt tolerant Black Liquor with Efficient Bacteria activity The ratio of sludge and compounding nutritive salt is 20:1, stand for standby use after addition.
3. a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge as claimed in claim 2 prepares the method that storage device is cultivated, special Sign is:PH in step 2 in cultivation reactor (1) is 6, temperature is 22 DEG C;The content of cultivation reactor (1) interior dissolved oxygen is 6mg/L;COD is 9.8g/L.
4. a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge as claimed in claim 2 prepares the method that storage device is cultivated, special Sign is:The N=1.
5. a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge as claimed in claim 4 prepares the method that storage device is cultivated, special Sign is:The volume of the cultivation reactor (1) is 200L;500 type of plate and frame filter press (2) model, volume 1m3; Storage mud bucket (3) volume is 200L.
6. a kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge as claimed in claim 4 prepares the method that storage device is cultivated, special Sign is:The CFU/g10 of salt tolerant high-efficiency strain described in step 1 is 5.0x109.
CN201810414311.0A 2018-05-03 2018-05-03 A kind of salt tolerant Black Liquor with Efficient Bacteria activated sludge prepares storage device and breeding method Pending CN108611253A (en)

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Application publication date: 20181002