CN103496791A - Production method for compound microbial preparation for aquatic products - Google Patents
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- CN103496791A CN103496791A CN201310449207.2A CN201310449207A CN103496791A CN 103496791 A CN103496791 A CN 103496791A CN 201310449207 A CN201310449207 A CN 201310449207A CN 103496791 A CN103496791 A CN 103496791A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 230000000813 microbial effect Effects 0.000 title abstract description 6
- 150000001875 compounds Chemical class 0.000 title abstract description 4
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 27
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 27
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 27
- 241000589516 Pseudomonas Species 0.000 claims abstract description 26
- 241000894006 Bacteria Species 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 244000005700 microbiome Species 0.000 claims description 38
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 25
- 150000003839 salts Chemical class 0.000 claims description 25
- 241000726221 Gemma Species 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 239000010871 livestock manure Substances 0.000 claims description 5
- 238000012805 post-processing Methods 0.000 claims description 5
- 239000002195 soluble material Substances 0.000 claims description 5
- 241000194031 Enterococcus faecium Species 0.000 abstract description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 abstract description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 abstract description 2
- 241000187561 Rhodococcus erythropolis Species 0.000 abstract description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 23
- 241001465754 Metazoa Species 0.000 description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000003643 water by type Substances 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 3
- 241000694873 Paralichthyidae Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000209094 Oryza Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000001706 oxygenating effect Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000202383 Halomonas sulfidaeris Species 0.000 description 1
- 231100000570 acute poisoning Toxicity 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention aims to provide a production method for a compound microbial preparation for aquatic products. The production method comprises the following steps: respectively carrying out high-density liquid fermentation on strains of bacillus cereus, bacillus licheniformis, halophilic pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium to obtain ultra-concentrated bacterium powder; and then preparing according to a ratio to obtain the compound microbial preparation. The production process is simple in process, is applicable to most existing fermentation processes and equipment and is good for being popularized and applied in a large scale.
Description
Technical field
The present invention relates to technical field of microbe application, be specifically related to the production method of a kind of aquatic products with complex microorganism preparations.
Background technology
Usually under state of nature, the indigenous bacterium in water body, by self-purification, can maintain the normal material cycle of water body.But, along with the development of high-density aquiculture, the organic loading in aquaculture water is well beyond the self-purification capacity of original flora.The organic matter be not used effectively becomes the greatest contamination source in water body, consume a large amount of dissolved oxygens, cause oxygen debt in water body, and then suppress organic complete oxidation, form a large amount of incomplete oxidation intermediates, and these incomplete oxidation products, be again often the maximum toxic substance of harm in aquaculture, as ammonia nitrogen, nitrite and hydrogen sulfide etc., cause the chronic and acute poisoning of cultivated animals, serious large-scale dead fish, the financial loss heaviness of causing.
In recent years, microbial preparation starts extensively should be in the cultivation field, for purifying water body, improve cultivated animals food consumption, reduce the generation of disease etc.The beneficial microorganism of rendering in water body is bred by raised growth, secrete abundant extracellular enzyme, organism is effectively decomposed, microorganism is in the growth metabolism process, can utilize ammonia nitrogen, the energy metabolism nitrite, can oxidation of sulfureted hydrogen etc., thereby realizes the degraded toxic substance, purify water, improve the purpose of culture benefit.
The microbial preparation of purifying water body at present commonly used single bacterial strain or the two or three kind of bacterial strain compound action of adopting more, viable count in preparation is on the low side, can not comprehensively, effectively improve the quality of aquaculture water, therefore, the task of top priority at present is the novel complex micro organism fungicide of active development, improves the cultivation present situation comprehensively.
Summary of the invention
The present invention is for solving the prior art problem, and a kind of complex microorganism preparations and production method thereof that can purifying water body be provided.Said preparation, by the collaborative promoter action of multiple-microorganism, has significant water transfer look, falls ammonia nitrogen, nitrite, enhances the transparency, and promotes the functions such as cultivated animals growth.
The applicant finds by long-term screening study, bacillus cereus, Bacillus licheniformis, have a liking between salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris and faecium and can produce synergy, ammonia nitrogen, nitrite in effective degraded water body, improve water quality, thereby facilitate the present invention.
One aspect of the present invention provides a kind of complex microorganism preparations, by bacillus cereus (
bacillus cereus), Bacillus licheniformis (
bacillus licheniformis), have a liking for the salt pseudomonas (
halomonas sulfidaeris), Lactobacterium acidophilum (
lactobacillus acidophilus), red city rhodococcus (
rhodococcus erythropolis), Rhodopseudomonas palustris (
rhodopseudomonas palustris) and faecium (
enterococcus faecium) form.
The preferred bacillus cereus CGMCC1.10163 of component, Bacillus licheniformis CGMCC1.10257 in above-mentioned complex microorganism preparations, have a liking for salt pseudomonas CCTCC M 2013070, Lactobacterium acidophilum CGMCC1.3915, red city rhodococcus CGMCC1.3613, Rhodopseudomonas palustris CGMCC1.2352 and faecium CGMCC1.2334.
In above-mentioned complex microorganism preparations, each component and content thereof are respectively: bacillus cereus 20-50 part, Bacillus licheniformis 10-30 part, have a liking for salt pseudomonas 5-15 part, Lactobacterium acidophilum 1-5 part, red city rhodococcus 1-5 part, Rhodopseudomonas palustris 10-15 part, faecium 10-30 part.
In above-mentioned complex microorganism preparations, the content of each component is preferably respectively: 40 parts of bacillus cereuses, 25 parts of Bacillus licheniformis, have a liking for 10 parts of salt pseudomonass, 3 parts of Lactobacterium acidophilums, 3 parts of red city rhodococcus, 12 parts of Rhodopseudomonas palustriss, 22 parts of faeciums.
One aspect of the present invention provides the application of above-mentioned complex microorganism preparations in purification of water quality.
The consumption of above-mentioned complex microorganism preparations is 20-40 g/acre of rice, uses once week about.
The present invention provides the production method of above-mentioned complex microorganism preparations on the other hand, comprises the steps:
1) by above-mentioned bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, manure enterococcin strain and adopt respectively the high density liquid fermentation, by the zymotechnique post-processing technology, make gemma content up to 10
11-10
12the hyperconcentration bacterium powder of CFU/g;
2) by hyperconcentration bacterium powder according to bacillus cereus 20-50 part, Bacillus licheniformis 10-30 part, have a liking for salt pseudomonas 5-15 part, Lactobacterium acidophilum 1-5 part, red city rhodococcus 1-5 part, Rhodopseudomonas palustris 10-15 part, faecium 10-30 part and prepared;
3) adopt water-soluble material that the complex microorganism preparations made is packed.
beneficial effect
In complex microorganism preparations of the present invention, viable count is up to 8 * 10
11cFU/g, can be widely used in fresh water, sea farming waters.Ammonia nitrogen, the nitrite that by the synergy between bacterial strain, can effectively degrade in water body, suppress the generation of wawter bloom, thereby purifying aquatic water improves the growing environment of cultivated animals, improves production performance.Production method provided by the invention is simple, is adapted to the existing zymotechnique of great majority and equipment, is conducive to large-scale promotion application.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only are not used in and limit the scope of the invention for the present invention is described.Should be understood that in addition after having read content of the present invention, those skilled in the art can make various changes or modification to the present invention, and these equivalent form of values fall into the application's appended claims limited range equally.
The all ingredients the present invention relates to and equipment, all can be selected from commercially available any one; The present invention relates in bacterial strain bacillus cereus purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.10163; Bacillus licheniformis is purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.10257; Having a liking for the salt pseudomonas is Qingdao Weilan Biology Group Co., Ltd.'s patented strain, deposit number CCTCC M 2013070; Lactobacterium acidophilum is purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.3915; Red city rhodococcus is purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.3613; Rhodopseudomonas palustris is purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.2352; Faecium is purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.2334.
embodiment 1
A kind of complex microorganism preparations, by bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, faecium and form, each component and content thereof are respectively: 20 parts of bacillus cereuses, 30 parts of Bacillus licheniformis, have a liking for 15 parts of salt pseudomonass, 5 parts of Lactobacterium acidophilums, 5 parts of red city rhodococcus, 10 parts of Rhodopseudomonas palustriss, 30 parts of faeciums.
The production method of above-mentioned complex microorganism preparations, comprise the steps:
1) by above-mentioned bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, manure enterococcin strain and adopt respectively the high density liquid fermentation, by the zymotechnique post-processing technology, make gemma content up to 10
11-10
12the hyperconcentration bacterium powder of CFU/g;
2) by hyperconcentration bacterium powder according to 20 parts of bacillus cereuses, 30 parts of Bacillus licheniformis, have a liking for 30 parts of 15 parts of salt pseudomonass, 5 parts of Lactobacterium acidophilums, 5 parts of red city rhodococcus, 10 parts of Rhodopseudomonas palustriss, faecium and prepared;
3) adopt water-soluble material that the complex microorganism preparations made is packed.
embodiment 2
A kind of complex microorganism preparations, by bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, faecium and form, each component and content thereof are respectively: 50 parts of bacillus cereuses, 10 parts of Bacillus licheniformis, have a liking for 5 parts of salt pseudomonass, 1 part of Lactobacterium acidophilum, 1 part of red city rhodococcus, 15 parts of Rhodopseudomonas palustriss, 10 parts of faeciums.
The production method of above-mentioned complex microorganism preparations, comprise the steps:
1) by above-mentioned bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, manure enterococcin strain and adopt respectively the high density liquid fermentation, by the zymotechnique post-processing technology, make gemma content up to 10
11-10
12the hyperconcentration bacterium powder of CFU/g;
2) by hyperconcentration bacterium powder according to 50 parts of bacillus cereuses, 10 parts of Bacillus licheniformis, have a liking for 10 parts of 5 parts of salt pseudomonass, 1 part of Lactobacterium acidophilum, 1 part of red city rhodococcus, 15 parts of Rhodopseudomonas palustriss, faecium and prepared;
3) adopt water-soluble material that the complex microorganism preparations made is packed.
embodiment 3
A kind of complex microorganism preparations, by bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, faecium and form, each component and content thereof are respectively: 40 parts of bacillus cereuses, 25 parts of Bacillus licheniformis, have a liking for 10 parts of salt pseudomonass, 3 parts of Lactobacterium acidophilums, 3 parts of red city rhodococcus, 12 parts of Rhodopseudomonas palustriss, 22 parts of faeciums.
The production method of above-mentioned complex microorganism preparations, comprise the steps:
1) by above-mentioned bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, manure enterococcin strain and adopt respectively the high density liquid fermentation, by the zymotechnique post-processing technology, make gemma content up to 10
11-10
12the hyperconcentration bacterium powder of CFU/g;
2) by hyperconcentration bacterium powder according to 40 parts of bacillus cereuses, 25 parts of Bacillus licheniformis, have a liking for 22 parts of 10 parts of salt pseudomonass, 3 parts of Lactobacterium acidophilums, 3 parts of red city rhodococcus, 12 parts of Rhodopseudomonas palustriss, faecium and prepared;
3) adopt water-soluble material that the complex microorganism preparations made is packed.
embodiment 4
The complex microorganism preparations that above-described embodiment 1-3 is made carries out live bacterial count, and experimental result is as shown in table 1:
In table 1 complex microorganism preparations, viable count relatively
| Sample | Viable count |
| Embodiment 1 | 5×10 11CFU/g |
| Embodiment 2 | 2×10 11CFU/g |
| Embodiment 3 | 8×10 11CFU/g |
the application of embodiment 5 complex microorganism preparations in lefteye flounder cultivation waters
Select two lefteye flounder culturing pools that the water body environment feature is more close, water surface area is 1 mu, and approximately 1.5 meters of the depth of waters, be divided into control group and test group.Control group is left intact, and the complex microorganism preparations that test group prepares the above embodiment of the present invention by 25 g/acres of rice consumptions is directly shed near oxygenating machine, starts oxygenating machine 1 hour, and microbial preparation is fully scattered, and action effect is more abundant.Every other day the same period is got respectively control group and the test group water sample carries out water quality detection, continuous detecting 7 days, and result is as described in Table 2.
Table 2 lefteye flounder cultivation waters water quality detection result
"-" means not detect.
As can be seen from Table 2, complex microorganism preparations of the present invention can significantly improve the transparency of cultivation waters water body, and the objectionable impuritiess such as degradation of ammonia nitrogen, nitrite and hydrogen sulfide, alleviate environmental pollution, for cultivated animals provides healthy growing environment, improve the production performance of cultivated animals.
Claims (4)
1. the production method of a complex microorganism preparations, comprise the steps:
1) by above-mentioned bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, manure enterococcin strain and adopt respectively the high density liquid fermentation, by the zymotechnique post-processing technology, make gemma content up to 10
11-10
12the hyperconcentration bacterium powder of CFU/g;
2) by hyperconcentration bacterium powder according to bacillus cereus 20-50 part, Bacillus licheniformis 10-30 part, have a liking for salt pseudomonas 5-15 part, Lactobacterium acidophilum 1-5 part, red city rhodococcus 1-5 part, Rhodopseudomonas palustris 10-15 part, faecium 10-30 part and prepared;
3) adopt water-soluble material that the complex microorganism preparations made is packed.
2. production method as claimed in claim 1, it is characterized in that, described bacillus cereus, Bacillus licheniformis, have a liking for salt pseudomonas, Lactobacterium acidophilum, red city rhodococcus, Rhodopseudomonas palustris, faecium and be respectively bacillus cereus CGMCC1.10163, Bacillus licheniformis CGMCC1.10257, have a liking for salt pseudomonas CCTCC M 2013070, Lactobacterium acidophilum CGMCC1.3915, red city rhodococcus CGMCC1.3613, Rhodopseudomonas palustris CGMCC1.2352 and faecium CGMCC1.2334.
3. production method as claimed in claim 1, it is characterized in that step 2) by hyperconcentration bacterium powder according to 40 parts of bacillus cereuses, 25 parts of Bacillus licheniformis, have a liking for 22 parts of 10 parts of salt pseudomonass, 3 parts of Lactobacterium acidophilums, 3 parts of red city rhodococcus, 12 parts of Rhodopseudomonas palustriss, faecium and prepared.
4. the complex microorganism preparations that the described production method of the arbitrary claim of claim 1-3 prepares.
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| CN104186960A (en) * | 2014-07-02 | 2014-12-10 | 广州大学 | Epinephelus coioides feed additive and application method thereof |
| CN104611279A (en) * | 2015-03-02 | 2015-05-13 | 中蓝连海设计研究院 | Rhodococcus erythropolis LH-N13 as well as microbial agent and use thereof |
| CN105918620A (en) * | 2016-05-06 | 2016-09-07 | 江苏东南生物科技有限公司 | Microbial agent for aquatic product feeds |
| CN108179113A (en) * | 2018-01-23 | 2018-06-19 | 黄永根 | A kind of production hydrogen composite biological agent |
| CN108179112A (en) * | 2018-01-23 | 2018-06-19 | 黄永根 | The method of chlorella pyrenoidosa joint mushroom production hydrogen |
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| CN104186960A (en) * | 2014-07-02 | 2014-12-10 | 广州大学 | Epinephelus coioides feed additive and application method thereof |
| CN104611279A (en) * | 2015-03-02 | 2015-05-13 | 中蓝连海设计研究院 | Rhodococcus erythropolis LH-N13 as well as microbial agent and use thereof |
| CN104611279B (en) * | 2015-03-02 | 2017-06-09 | 中蓝连海设计研究院 | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes |
| CN105918620A (en) * | 2016-05-06 | 2016-09-07 | 江苏东南生物科技有限公司 | Microbial agent for aquatic product feeds |
| EP3649233A4 (en) * | 2017-07-03 | 2020-12-16 | Oakbio Inc. | Novel microbial biomass based feed products |
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| US11439172B2 (en) | 2017-07-03 | 2022-09-13 | Oakbio, Inc. | Microbial biomass based feed products |
| WO2019010116A1 (en) | 2017-07-03 | 2019-01-10 | Oakbio Inc. | Novel microbial biomass based feed products |
| JP2020526230A (en) * | 2017-07-03 | 2020-08-31 | オークバイオ インコーポレイテッドＯａｋｂｉｏ Ｉｎｃ． | Feed products based on new microbial biomass |
| CN108179112B (en) * | 2018-01-23 | 2021-05-18 | 杭州富阳佳畅机械有限公司 | Method for producing hydrogen by chlorella pyrenoidosa combined bacteria |
| CN108179113B (en) * | 2018-01-23 | 2021-05-18 | 杭州富阳佳畅机械有限公司 | Hydrogen-producing composite biological agent |
| CN108179112A (en) * | 2018-01-23 | 2018-06-19 | 黄永根 | The method of chlorella pyrenoidosa joint mushroom production hydrogen |
| CN108179113A (en) * | 2018-01-23 | 2018-06-19 | 黄永根 | A kind of production hydrogen composite biological agent |
| CN109497343A (en) * | 2018-11-09 | 2019-03-22 | 南宁学院 | A kind of Pangasius sutchi disease-resistant growth-promoting preparation |
| CN111909865A (en) * | 2020-07-06 | 2020-11-10 | 薛松晓 | Compound microbial preparation for air deodorization and application thereof |
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