CN103352018B - Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation - Google Patents
Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation Download PDFInfo
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Abstract
The invention provides a compound microorganism preparation used for freshwater aquiculture water body improvement, which comprises the raw materials by weight percentage: 25-30wt% of bacillus subtilis, 25-30wt% of bacillus amyloliquefaciens and 15-20wt% of thiobacillus denitrificans. The invention further provides a preparation method of the microorganism preparation, which comprises the steps that bacillus subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluids are sequentially inoculated into a culture medium and fermented; an obtained bacterium fluid is mixed with a vector and a nutritional substance; and the microorganism preparation is obtained. The microorganism preparation can obviously improve the water quality of an aquiculture water body, and can improve the meat quality of an aquiculture animal.
Description
Technical field
The present invention relates to a kind of water improvement technical field, particularly relate to a kind of complex microorganism preparations for aquaculture water improvement and preparation method thereof.
Background technology
China is the first in the world aquaculture big country, and according to statistics, Chinese inland can water surface for aquaculture be 2,734 ten thousand hectares, and seawater can cultured area 4,380,000 hectares, and rice field fish culture area calendar year 2001 is 1,520,000 hectares.Especially the southern china region of rivers and lakes, aquaculture occupies critical role in agriculture production, and many areas all produce the important channel of getting rich using aquaculture as local farmers, and aquaculture had become one of mainstay industry cultivated in agricultural already gradually.
Meanwhile, China is also that aquaculture water pollutes one of the most serious country.The saying that China just has from ancient times " breed fish and first support water ", the quality of water quality is directly connected to the benefit of cultivation, and along with the development of aquaculture, high-density breeding needs to drop into a large amount of feeds, bait remnants and aquatic animal movement pollute aquaculture water, cause there is NH
3, H
2the objectionable impurities such as S, organism increases, and chemical oxygen demand (COD) and biological oxygen demand (BOD) (BOD) increase.At present, the method for multiple water treatment is had:
One, physical method
Machinery desilting is modal method, but mechanical desilting cost is high, and silt from the bottom of lake is not source of pollution to have scholar to think, desilting only can improve water quality in a short time; Refreshing the water periodically to culturing pool is also effective means, but cost is very high, and especially water resources is day by day in short supply, is difficult to carry out; Forward bottom current to upper strata, then discharge, can Eutrophic Extent be reduced, but water waste is serious; Utilize gelifying agent, plastic film covers bed mud, can internal load be reduced, but can water ecosystem be destroyed; The oxygen amount that exposure oxygenation can ensure needed for aquaculture organism, can prevent the amount reproduction of algae simultaneously, but generally be only applicable to small water, and needs and other Combination of Methods is applied.In addition, patent CN101544412B discloses a kind of material using mineral material and modified product composition thereof, to adsorb the objectionable impuritiess such as ammonia nitrogen, phosphorus, organism and heavy metal, but this patent uses a large amount of organic modifiers, other pollutent can be incorporated in water body.
Two, chemical process
Chemical agent can in-SH the radical reaction forming microprotein halfcystine,-SH base is made to be the enzymatic inactivation of active site, thus the cell walls of destroy microorganisms, cytolemma and internal substance, thus kill cell, that commonly uses at present has copper sulfate, copper chelate, chlorine or oxychloride etc.; As patent CN102730783A discloses the aquaculture water modifying agent of aluminum chloride, Tai-Ace S 150, gac composition, in addition, patent CN101481170A also discloses the application of thionamic acid in degrading nitrite and reduction water pH value.But water used for aquiculture and environment are subject to severe contamination in recent years, the situation of aquatic products medication is more and more serious, and cause pathogenic bacterium to produce resistance, aquaculture organism medicine residual exceeds standard, and brings some diseases to human consumer.
Three, bioelectric detecting and biological restoration
Microbial preparation is utilized to improve water quality, reduce and control the content of ammonia, nitrite in aquaculture water, and pathogenic micro-organism Growth and reproduction can be controlled, to improve the resistibility of animal, biotechnological formulation is harmless, have no side effect, the continuous action time is long, and be otherwise known as " beneficial microorganism " or " probiotics ".The application of current microbial preparation in aquatic products is more and more extensive.
Commercial applications is photosynthetic bacterium the earliest, for regulating water quality, then, on this basis, in succession develops multiple-microorganism again, and the mixed culture of microorganism and synergy also more and more receive the concern of people.Xue Heng equality (fodder industry, 1997,18 (2): 23-26) uses genus bacillus, photosynthetic bacterium and Bdellovibrio composite fungus agent, finds that prawn virus disease sends out time lag; Photosynthetic bacterium is applied to foster soft-shelled turtle pond by Ye Yizuo etc. (aquatic science and technology information, 1996,23 (2): 51-55) together with actinomycetes, and COD clearance can reach more than 90%; Official to promote culture etc. (Chinese aquatic science, 2000,7 (2): 51-55) build bacterium, with photosynthetic bacterium, water quality improvement carried out to foster soft-shelled turtle pond with beautiful, dissolved oxygen, reduction COD and ammonia nitrogen can be increased; Luo Yongsheng etc. (agro-environment science journal, 2006,25 volume supplementary issue: 206-210) use photosynthetic bacterium and genus bacillus, degradable water body COD, and the two synergy obviously; Patent CN1266054C discloses the mixture of a kind of composite fungus agent and micro-algae, and bacterial classification comprises thiobacillus denitrificans, nitrobacteria, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, actinomycetes, streptomycete, genus bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria; Patent CN1226202C discloses a kind of microbial preparation for purifying water body, comprises nitrobacteria, nitrite bacteria, denitrifying bacterium, sulfide-oxidizing enzyme, photosynthetic bacterium.
Applied although microniological proudcts are existing in aquaculture, still there is preparation complexity, high in cost of production problem at present, need the new microbial preparation for aquaculture water improvement.
Summary of the invention
To achieve these goals, improve the water quality of aquaculture water, and improve the meat of cultivated animals, the invention provides a kind of microbial preparation for aquaculture water improvement, it comprises following technical scheme:
The present invention first aspect is to provide a kind of microbial preparation for freshwater aquiculture water improvement, and according to weight percent, raw material comprises following component:
Subtilis (Bacillus subtilis) 25-30wt%;
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) 25-30wt%;
Thiobacillus denitrificans (Thiobacillus denitrificans) 15-20wt%.
The complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, the microorganism of freshwater aquiculture water improvement can also be can be used for containing other, as Rhodopseudomonas palustris, Bu Ladi yeast etc., but be preferably made up of subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans, and do not comprise other bacterial classifications.
Wherein, with the gross weight of described microorganism for benchmark, the weight percent of subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans is respectively 25-30wt%, 25-30wt%, 15-20wt%, is preferably 20-25wt%, 24-32wt%, 10-18wt%.
The complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, viable count is preferably >=30 hundred million/g.
The complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, microbial preparation can be liquid preparation or solid preparation, be preferably solid preparation, be more preferably particle and/or powder, median size is preferably 10-100 order, be more preferably 20-80 order, be more preferably 30-70 order, most preferably be 40-60 order, as 50 orders.
The complex microorganism preparations of the aquaculture water improvement that the present invention is above-mentioned, wherein, microbial preparation can also comprise carrier, and described carrier can be liquid vehicle, as water; Also can be solid carrier, as any one or a few the mixture in zeolite, calcium carbonate, kaolin (comprising calcined kaolin), wilkinite.
The complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, can also comprise nutritive substance, described nutritive substance can be selected from any one or a few the mixture in starch (being preferably W-Gum), molasses, trehalose, sucrose, maltose, isomaltose, wood sugar, fructose, stachyose, raffinose.
According to a kind of preferred embodiment of complex microorganism preparations of the present invention, wherein, with the gross weight of carrier (be preferably solid carrier) and nutritive substance for benchmark, preferably, the weight percent of carrier is 80-90w%, and the weight percent of nutritive substance is 10-20wt%.
In the preferred embodiment of complex microorganism preparations of the present invention, wherein, described carrier is the combination of zeolite powder and calcium carbonate, and described nutritive substance is the combination of W-Gum and molasses; Wherein, preferably, zeolite powder weight percent is 65-75wt%, and calcium carbonate weight percent is 10-20wt%; The weight percent of W-Gum is 5-15wt%, and the weight percent of molasses is 1-10wt%.
The present invention second aspect is to provide a kind of method preparing the above-mentioned complex microorganism preparations for aquaculture water improvement, said method comprising the steps of:
Step 1, provides subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluid;
Step 2, subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluid are inoculated in substratum successively, fermentation, then concentrate and obtain high-concentration bacterial liquid;
Step 3, gained high-concentration bacterial liquid mixes with carrier, nutritive substance, obtains described microbial preparation.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, in step 1, the preparation method of subtilis, bacillus amyloliquefaciens seed culture fluid preferably cultivates respectively under 30-35 ° of C condition, obtains described seed culture fluid.Culturing process culture medium matrix component used is preferably and is made up of the water of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium-chlor 0.2-0.25wt%, ferric sulfate 30-35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% and surplus, and the pH value of substratum is 6.5-6.8.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, in step 1, the incubation time of subtilis, bacillus amyloliquefaciens is preferably 36-54 hour, be more preferably 40-55 hour, most preferably be 45-50 hour, as 47 hours, 48 hours; The incubation time of thiobacillus denitrificans is preferably 5-10 days, is more preferably 6-9 days, most preferably is 6-8 days.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, in step 1, the preparation method of thiobacillus denitrificans seed culture fluid preferably cultivates under 29-33 ° of C and intensity of illumination are preferably 4500-10000lx condition, obtains described seed culture fluid.Culturing process culture medium matrix component used is preferably by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, the water composition of peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt% and surplus, the pH value of substratum is 6.5-6.8.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, in step 2, described nutrient media components is preferably by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, the water composition of peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt% and surplus, pH value is 6.5-6.8.
Wherein, in above-mentioned nutrient media components, growth factor solution preferably comprises the vitamin H aqueous solution of 2-2.3wt% mass concentration, the vitamins B aqueous solution of 12-15wt% mass concentration.Described vitamins B can be any one or mixture multiple arbitrarily in vitamin B group.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, in described seeded process, inoculum size is preferably the 2%-5% of substratum gross weight described in step 2.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, in described fermenting process, temperature preferably controls arbitrary temp within the scope of 35-40 ° of C or temperature range, mixing speed is preferably 200r/min-280r/min, air flow be carry out under the condition being preferably 1: 1, the time is preferably and continues 30-50 hour, be more preferably 33-46 hour, most preferably be 36-42 hour.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, described concentrating is carried out preferably by centrifugation, and the rotating speed of centrifugation is preferably 3000-5000r/min.
The preparation method of the microbial preparation for aquaculture water improvement that the present invention is above-mentioned, wherein, described carrier is solid carrier, as the mixture of any one or a few in zeolite, calcium carbonate, kaolin, wilkinite.
The preparation method of the complex microorganism preparations for aquaculture water improvement that the present invention is above-mentioned, wherein, described nutritive substance can be selected from any one or a few the mixture in starch (being preferably W-Gum), molasses, trehalose, sucrose, maltose.
According to the first preferred embodiment of preparation method of the above-mentioned complex microorganism preparations for aquaculture water improvement of the present invention, with the gross weight of carrier (be preferably solid carrier) and nutritive substance for benchmark, the weight percent of carrier is 70-90w%, and the weight percent of nutritive substance is 10-30wt%.
In the first preferred embodiment of the preparation method of the complex microorganism preparations for aquaculture water improvement stated on the invention, described carrier is zeolite powder and calcium carbonate, and described nutritive substance is W-Gum and molasses; Wherein, zeolite powder weight percent is 60-70wt%, and calcium carbonate weight percent is 10-20wt%; The weight percent of W-Gum is 5-15wt%, and the weight percent of molasses is 5-15wt%.
According to the second preferred embodiment of preparation method of the above-mentioned complex microorganism preparations for aquaculture water improvement of the present invention, with the gross weight of described microbial preparation for benchmark, the weight percent of subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans is respectively 15-40wt%, 15-40wt%, 10-20wt%.
According to the third preferred embodiment of preparation method of the above-mentioned complex microorganism preparations for aquaculture water improvement of the present invention, in step 3, after granulation, carry out drying, and to be preferably dried to moisture content be 5-7%.
According to the 4th kind of preferred embodiment of preparation method of the above-mentioned complex microorganism preparations for aquaculture water improvement of the present invention, also comprise step 4: pulverized by step 3 gained mixture, the temperature in crushing process controls arbitrary temp within the scope of 10-45 ° of C or temperature range.
In the 4th kind of preferred embodiment of the preparation method of the complex microorganism preparations for aquaculture water improvement stated on the invention, the step of carrying out screening can also be comprised after pulverizing, described screening is preferably and adopts 10-100 mesh sieve to implement, be more preferably 20-80 mesh sieve, be more preferably 30-70 mesh sieve, be more preferably 40-60 mesh sieve, as 50 mesh sieves.
Above-mentioned technical scheme tool has the following advantages or beneficial effect:
Complex microorganism preparations for aquaculture water improvement provided by the invention, water body BOD, ammonia-nitrogen content, nitrite content and pH value can be reduced fast, increase dissolved oxygen amount, can realize throwing in breeding process, and the meat of cultivated animals can be improved; The method of the described microbial preparation of preparation provided by the invention, can obtain the microbial preparation of high viable bacteria content, and preparation method is simple, and the kind of required bacterial classification is few, and cost is low.
Embodiment
Below describe several preferred embodiment of the present invention in detail:
Embodiment 1
Raw material forms:
Preparation method:
Step 1,
Aseptically be inoculated in the first substratum by subtilis and bacillus amyloliquefaciens respectively, 30 ° of C-35 ° of C, single spawn culture 48 hours, obtain subtilis and bacillus amyloliquefaciens seed culture fluid, its viable count is all greater than 5.0 × 10
9cfu/ml.Wherein, first substratum comprises the aqueous solution of vitamin H 2-2.3wt%, vitamin B12-15wt% by molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium-chlor 0.2-0.25wt%, ferric sulfate 30-35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt%() and the water of surplus form, pH value is 6.5-6.8.
Aseptically be inoculated in by thiobacillus denitrificans in the second substratum, intensity of illumination is 4500-10000lx, temperature is under 29 ° of C-33 ° of C conditions, cultivate 6-8 days, obtain thiobacillus denitrificans seed culture fluid, its viable count is greater than 5.0 × 109cfu/ml.Wherein, second substratum is by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt%(comprise the aqueous solution of vitamin H 2-2.3wt%, vitamin B complexes 12-15wt%) and surplus water composition, pH value is 6.5-6.8.
Step 2,
The subtilis seed culture fluid obtained in step 1, bacillus amyloliquefaciens seed culture fluid, thiobacillus denitrificans seed culture fluid are aseptically inoculated into successively in proportion in the 3rd substratum, inoculum size is the 2%-5% of this substratum total amount.
35-40 ° of C, mixing speed be 200r/min-280r/min, under air flow is the condition of 1: 1, fermentation 36-42 hour, the viable count obtaining subtilis is greater than 3.0 × 10
9the viable count of cfu/ml, bacillus amyloliquefaciens is greater than 3.0 × 10
9the viable count of cfu/ml, thiobacillus denitrificans is greater than 1.0 × 10
9the fermented liquid of cfu/ml; By fermented liquid high speed centrifugation under the rotating speed of 3000-5000r/min, obtain the concentrated bacterium liquid of high density as mother liquor.
Wherein, 3rd substratum is by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt%(comprise the aqueous solution of vitamin H 2-2.3wt%, vitamin B complexes 12-15wt%) and surplus water composition, pH value is 6.5-6.8.
Step 3,
Moved into by concentrated bacterium liquid in the matrix of zeolite powder+feed calcium carbonate+W-Gum+molasses composition and mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, with matrix (carrier and nutritive substance) gross weight for benchmark, matrix is made up of zeolite powder 70wt%, calcium carbonate 15wt%, W-Gum 10wt%, molasses 5wt%.
Step 4,
By the material obtained in step 3 in temperature not higher than 45 DEG C and under being not less than the condition of 10 DEG C, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, obtains moisture content≤6%, the microbial preparation for freshwater aquiculture water improvement that viable count is 3,000,000,000/g.
Embodiment 2
Raw material forms:
Preparation method:
Step 1,
According to the method described in embodiment 1, provide subtilis seed culture fluid, bacillus amyloliquefaciens seed culture fluid, thiobacillus denitrificans seed culture fluid.
Step 2,
According to the method described in embodiment 1, obtain the concentrated bacterium liquid of high density as mother liquor.
Step 3,
Moved into by concentrated bacterium liquid in the matrix of zeolite powder+feed calcium carbonate+W-Gum+molasses composition and mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, with matrix (carrier and nutritive substance) gross weight for benchmark, matrix is made up of zeolite powder 75wt%, calcium carbonate 10wt%, W-Gum 8wt%, molasses 7wt%.
Step 4,
By the material obtained in step 3 in temperature not higher than 45 DEG C and under being not less than the condition of 10 DEG C, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, obtains moisture content≤6%, the microbial preparation for freshwater aquiculture water improvement that viable count is 3,000,000,000/g.
Embodiment 3
Raw material forms:
Preparation method:
Step 1,
According to the method described in embodiment 1, provide subtilis seed culture fluid, bacillus amyloliquefaciens seed culture fluid, thiobacillus denitrificans seed culture fluid.
Step 2,
According to the method described in embodiment 1, obtain the concentrated bacterium liquid of high density as mother liquor.
Step 3,
Moved into by concentrated bacterium liquid in the matrix of zeolite powder+feed calcium carbonate+W-Gum+molasses composition and mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, with matrix (carrier and nutritive substance) gross weight for benchmark, matrix is made up of zeolite powder 65wt%, calcium carbonate 15wt%, W-Gum 5wt%, molasses 5wt%.
Step 4,
By the material obtained in step 3 in temperature not higher than 45 DEG C and under being not less than the condition of 10 DEG C, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, obtains moisture content≤6%, the microbial preparation for freshwater aquiculture water improvement that viable count is about 3,000,000,000/g.
Embodiment 4
Raw material forms:
Preparation method:
Step 1:
According to the method described in embodiment 1, provide subtilis seed culture fluid, bacillus amyloliquefaciens seed culture fluid, thiobacillus denitrificans seed culture fluid.
Step 2,
According to the method described in embodiment 1, obtain the concentrated bacterium liquid of high density as mother liquor.
Step 3,
Moved into by concentrated bacterium liquid in the matrix of zeolite powder+feed calcium carbonate+W-Gum+molasses composition and mix, mixed fluid is granulated on wobbler, then carries out fluidized drying, and dried biodiversity per-cent is 5-7%.
Wherein, with matrix (carrier and nutritive substance) gross weight for benchmark, matrix is made up of zeolite powder 70wt%, calcium carbonate 10wt%, W-Gum 10wt%, molasses 10wt%.
Step 4,
By the material obtained in step 3 in temperature not higher than 45 DEG C and under being not less than the condition of 10 DEG C, proceed between pulverizing and pulverize, after 50 eye mesh screens, after sieve, coarse fodder is pulverized again, obtains moisture content≤6%, the microbial preparation for freshwater aquiculture water improvement that viable count is about 3,000,000,000/g.
Microbial preparation provided by the invention uses in breeding process, when not affecting cultivated animals, after one month, in water body, the horizontal range of decrease >=45% of BOD, the ammonia-nitrogen content range of decrease >=30%, the nitrite content range of decrease >=40%, dissolved oxygen amount increased value >=28%, pH value drop to 7.8-8.1, in cultivated animals, protein content increased value >=3%, the lipid content range of decrease >=2%.And, microbial preparation provided by the present invention, still there is function after six months that improve water quality He improve cultivated animals meat, after six months, in water body, the horizontal range of decrease >=80% of BOD, the ammonia-nitrogen content range of decrease >=70%, the nitrite content range of decrease >=80%, dissolved oxygen amount increased value >=55%, pH value drop to 7.4-7.8, in cultivated animals, protein content increased value >=5%, the lipid content range of decrease >=3%.
Complex microorganism preparations for freshwater aquiculture water improvement provided by the invention, water body BOD, ammonia-nitrogen content, nitrite content and pH value can be reduced fast, increase dissolved oxygen amount, can realize throwing in breeding process, and the meat of cultivated animals can be improved; The method of the described microbial preparation of preparation provided by the invention, can obtain the microbial preparation of high viable bacteria content, and preparation method is simple, and the kind of required bacterial classification is few, and cost is low.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (10)
1. for a complex microorganism preparations for freshwater aquiculture water improvement, it is characterized in that, according to weight percent, microorganism is:
Subtilis 25-30wt%;
Bacillus amyloliquefaciens 25-30wt%;
Thiobacillus denitrificans 15-20wt%.
2. complex microorganism preparations according to claim 1, is characterized in that, also comprises carrier and/or nutritive substance.
3. complex microorganism preparations according to claim 2, is characterized in that, described carrier is selected from any one or a few the mixture in zeolite, calcium carbonate, kaolin, wilkinite.
4. complex microorganism preparations according to claim 2, it is characterized in that, described nutritive substance is selected from any one or a few the mixture in starch, molasses, trehalose, sucrose, maltose, isomaltose, wood sugar, fructose, stachyose, raffinose.
5. according to the complex microorganism preparations in claim 2-4 described in any one, it is characterized in that, comprise carrier and nutritive substance, and with carrier and nutritive substance gross weight for benchmark, the weight percent of carrier is 80-90w%, and the weight percent of nutritive substance is 10-20wt%.
6. microbial preparation according to claim 5, is characterized in that, described carrier is the combination of zeolite powder and calcium carbonate, and described nutritive substance is the combination of W-Gum and molasses; Wherein, with carrier and nutritive substance gross weight for benchmark, zeolite powder weight percent is 65-75wt%, and calcium carbonate weight percent is 10-20wt%; The weight percent of W-Gum is 5-15wt%, and the weight percent of molasses is 5-15wt%.
7. prepare a method for microbial preparation as claimed in claim 1, it is characterized in that, comprise the steps:
Step 1, provides subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluid;
Step 2, subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluid are inoculated in substratum successively, fermentation, then concentrate and obtain high-concentration bacterial liquid;
Step 3, gained high-concentration bacterial liquid mixes with carrier, nutritive substance, obtains described microbial preparation.
8. method according to claim 7, it is characterized in that, nutrient media components described in step 2 is by molasses 1-1.2wt%, corn steep liquor 0.8-1.0wt%, the water composition of peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, potassium primary phosphate 0.3-0.5wt%, Sodium phosphate dibasic 0.2-0.3wt%, magnesium sulfate 0.01-0.05wt%, growth factor solution 1-1.2wt% and surplus, pH value is 6.5-6.8; Wherein, growth factor solution comprises the vitamin H aqueous solution of 2-2.3wt% mass concentration, the vitamins B aqueous solution of 12-15wt% mass concentration.
9. method according to claim 8, is characterized in that, in fermenting process described in step 2, temperature controls arbitrary temp within the scope of 35-40 DEG C or temperature range.
10. method according to claim 7, is characterized in that, also comprises step 4: pulverized by step 3 gained mixture, and the temperature in crushing process controls arbitrary temp within the scope of 10-45 DEG C or temperature range.
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| CN1266054C (en) * | 2002-11-12 | 2006-07-26 | 上海创博生态工程有限公司 | Modifying agent for microorganism breeding water and preparing method thereof |
| CN101717724A (en) * | 2009-12-17 | 2010-06-02 | 天津市农业生物技术研究中心 | Compound microecological preparation used for water body purification of aquaculture |
| CN102583775B (en) * | 2012-02-14 | 2013-05-15 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
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