CN115784466A - Denitrifying agent for residual antibiotic-containing polluted water body in low-temperature environment and treatment method thereof - Google Patents
Denitrifying agent for residual antibiotic-containing polluted water body in low-temperature environment and treatment method thereof Download PDFInfo
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Abstract
The invention discloses a denitrifying agent for a residual antibiotic-containing polluted water body in a low-temperature environment and a treatment method thereof, belonging to the technical field of sewage treatment. The invention combines a first microbial inoculum and a second microbial inoculum consisting of sphingomonas, pandalata and Kurthia, performs denitrification treatment by adopting a biofilm reactor-biofilter process, and simultaneously adds zinc acetate in the biofilter treatment to realize the efficient removal of residual antibiotics and total nitrogen in wastewater under a low-temperature environment, thereby eliminating the inhibition of the residual antibiotics on microorganisms in the wastewater treatment and eliminating the potential risk of the residual antibiotics diffusing to the environment.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a denitrifying agent for a residual antibiotic-containing polluted water body in a low-temperature environment and a treatment method thereof.
Background
The wastewater discharged by livestock and poultry breeding industry and the like has complex components, high organic matter content and high ammonia nitrogen concentration, and generally has the characteristics of high nitrogen, residual antibiotics and the like. In winter in northern China, the outdoor temperature is low, which can cause that the inlet water temperature is too low in the wastewater treatment process, further cause that microorganisms in a wastewater treatment system proliferate and grow slowly, and influence the denitrification efficiency. Meanwhile, antibiotics are used in the culture process, so that part of antibiotic residues exist in the discharged fecal sewage, and the biological activity of denitrifying bacteria is further inhibited. At present, the existing low-temperature enhanced biological membrane denitrification methods comprise the following steps: optimizing process parameters (e.g., carrier, hydraulic retention time, etc.), adding chemicals (e.g., ferrous ions, rhamnolipids, etc.), adding denitrifying bacteria (e.g., aerobic denitrifying bacteria), etc.
The search shows that the Chinese patent application with application publication No. CN110745966A and application publication No. 2020, 2, month and 4 discloses a binary system of quorum sensing-aerobic denitrifying bacteria for enhancing biomembrane denitrification under low temperature condition, a construction method and application, firstly, aerobic denitrifying bacteria with high quorum sensing capability and high denitrification capability are separated from an aerobic biomembrane reactor which stably runs at low temperature; secondly, optimizing an optimal binary system consisting of the strain and quorum sensing bacteria with strong signal molecule production activity; finally, the binary system is fixed on a carrier and then is added into a biomembrane reactor to strengthen low-temperature denitrification. However, the present invention does not provide for treatment of residual antibiotics in wastewater discharged from livestock and poultry breeding industry. Therefore, it is difficult to achieve effective denitrification of wastewater under low temperature conditions for contaminated water bodies that are high in nitrogen and contain antibiotic residues.
Disclosure of Invention
1. Problems to be solved
Aiming at the problem that the denitrification treatment of high-nitrogen wastewater containing antibiotics is difficult to realize under the existing low-temperature condition, the invention provides a denitrifying agent for a water body polluted by residual antibiotics in a low-temperature environment and a treatment method thereof. The method comprises the steps of configuring a combination of a first microbial agent and a second microbial agent consisting of sphingomonas, pandalata and Kurthia, performing denitrification treatment by adopting a biofilm reactor-biofilter process, inoculating the first microbial agent into a primary biofilm reactor, inoculating the second microbial agent into a secondary biofilm reactor, and simultaneously adding zinc acetate in the biofilter treatment to realize efficient removal of residual antibiotics and total nitrogen in the wastewater in a low-temperature environment.
2. Technical scheme
In order to solve the problems, the technical scheme adopted by the invention is as follows:
the denitrifying agent for the polluted water body containing the residual antibiotics in the low-temperature environment comprises a first microbial agent and a second microbial agent, wherein the first microbial agent consists of sphingomonas, pandalata and Kurthia, the second microbial agent is aeromonas, and the mass ratio of the first microbial agent to the second microbial agent is 1: (2.5-3).
Preferably, the first microbial agent comprises 10 parts by mass of sphingomonas, 1 part by mass of panduraemia and 7 parts by mass of kurthia sp.
Preferably, the number of sphingomonas in the first bacterial agent is 10 9 ～10 10 The number of the bacteria is 10 in CFU/mL and Pandora 6 ～10 7 The number of CFU/mL and Kurthia sp is 10 8 ～10 9 CFU/mL。
The invention relates to a denitrification treatment method for a polluted water body containing residual antibiotics in a low-temperature environment, which comprises the steps of inoculating a first microbial inoculum into a primary biofilm reactor, inoculating a second microbial inoculum into a secondary biofilm filter, and respectively starting the primary biofilm reactor and the secondary biofilm filter; then, sequentially carrying out denitrification treatment on the high-nitrogen wastewater containing the antibiotics through a primary biomembrane reactor and a secondary biological filter to obtain a water body after denitrification treatment; wherein, in the process of treatment in the secondary biofilter, zinc acetate is added into the secondary biofilter, the first microbial inoculum consists of sphingomonas, pandalata and Kurthia, and the second microbial inoculum is aeromonas.
Preferably, the inoculation volume of the first microbial inoculum is 55-65% of the effective volume of the primary biofilm reactor, and the inoculation volume of the second microbial inoculum is 40-55% of the effective volume of the secondary biofilter.
Preferably, in the process of starting the primary biofilm reactor and the secondary biofilm filter, the dissolved oxygen concentration in the primary biofilm reactor and the secondary biofilm filter is controlled to be 1.5-2.5 mg/L respectively.
Preferably, the first microbial inoculum is inoculated into a primary biofilm reactor and subjected to field planting culture for 48-72 h; and inoculating the second microbial inoculum into the secondary biofilter, and planting and culturing for 72-84 h.
Preferably, in the process of sequentially carrying out denitrification treatment on the high-nitrogen wastewater containing the antibiotics through a primary biofilm reactor and a secondary biofilm filter, the pH value of the primary biofilm reactor is controlled to be 6.8-7.5, and the concentration of dissolved oxygen in the reactor is 3.5-4.5 mg/L; controlling the pH value of the secondary biological filter to be 7.8-8.5 and controlling the concentration of dissolved oxygen in the filter to be 2.8-3.5 mg/L.
Preferably, in the process of treatment in the secondary biofilter, zinc acetate is added into the secondary biofilter to maintain the concentration of zinc ions in the wastewater to be 150-300 mg/L.
Preferably, the primary biomembrane reactor is filled with polypropylene composite filler, the filling rate of the polypropylene composite filler is 25-35%, the polypropylene composite filler consists of plastic rings and fiber bundles, the plastic rings are made of polypropylene materials, and the fiber bundles are made of hydroformylation fiber materials; and a zeolite filter material is filled in the secondary biological filter, and the filling rate of the zeolite filter material is 20-25%.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
(1) The denitrifying agent for the low-temperature environment polluted water body containing the residual antibiotics comprises a first microbial agent consisting of sphingomonas, pandora and Kurthia and a second microbial agent consisting of aeromonas, and is configured and combined with the low-temperature resistant bacteria, so that extracellular polymers can be generated under the low-temperature condition, a biological film is easy to form, and effective ammonia nitrogen conversion and antibiotic degradation can be realized;
(2) According to the denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment, the denitrification treatment is carried out by adopting a combined process of the biofilm reactor and the biological filter, which is completely different from the traditional A/O denitrification treatment process, glucose or methanol and the like do not need to be additionally added as a carbon source, and the return treatment of the nitrified liquid is also not needed;
(3) Compared with the traditional activated sludge process for denitrification treatment, the denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment can realize high-efficiency denitrification of a biochemical treatment system under the low-temperature condition, maintain the treatment efficiency at a higher level, realize high-efficiency degradation and removal of the residual antibiotics in the wastewater, remove the inhibition effect of the residual antibiotics on the biochemical system, and eliminate the pollution risk of the residual antibiotics discharged to the environment.
Drawings
FIG. 1 is a schematic flow diagram of a denitrification treatment method for a residual antibiotic-containing polluted water body in a low-temperature environment according to the present invention.
Detailed Description
As shown in figure 1, the denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment mainly comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient gravy agar culture medium, culturing to logarithmic growth phase, mixing 3 bacterial solutions, and controlling the amount of Sphingomonas in the mixed bacterial solution to 10 9 ～10 10 The number of the bacteria is 10 in CFU/mL and Pandora 6 ～10 7 The number of CFU/mL and Kurthia sp is 10 8 ～10 9 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum;
inoculating aeromonas to an RS culture medium, culturing until the logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 9 ～10 10 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 55-65% of the effective volume of the primary biofilm reactor, and the rest volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. The primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 25-35%, the polypropylene combined filler consists of a plastic ring and a fiber bundle, the plastic ring is made of polypropylene materials, and the fiber bundle is made of hydroformylation fiber materials; controlling the dissolved oxygen concentration in the primary biofilm reactor to be 1.5-2.5 mg/L, and completing field planting culture for 48-72 h;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 40-55% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. And zeolite filter materials are filled in the secondary biological filter, the filling rate of the zeolite filter materials is 20-25%, the dissolved oxygen concentration in the secondary biological filter is controlled to be 1.5-2.5 mg/L, the field planting culture is carried out for 72-84 h, and the secondary biological filter is started. Wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1: (2.5-3).
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is below 10 ℃, preferably 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3-1/4 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 6.8-7.5, and the concentration of dissolved oxygen in the reactor is 3.5-4.5 mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 7.8-8.5, the concentration of dissolved oxygen in the filter is 2.8-3.5 mg/L, and zinc acetate is added into the secondary biological filter to maintain the concentration of zinc ions in the wastewater to be 150-300 mg/L.
It should be noted that the number of the Sphingomonas used in the invention is CGMCC 1.15275, the number of the Pandora is CGMCC 1.4968, the number of the Kutt's strain is CGMCC 1.15268, and the number of the Aeromonas is CGMCC 1.1816, which can be purchased commercially. The aerobic activated sludge is conventional aerobic activated sludge sold in the market.
In the primary biomembrane reactor, sphingomonas, panduraceae and Kurthia are all low-temperature resistant bacteria which can grow and propagate under the low-temperature condition. Meanwhile, the three bacteria can produce extracellular polymeric substances, so that a biomembrane is easily formed, and the biofilm formation in the biomembrane reactor is conveniently finished. Sphingomonas and Pandora have high ammonia monooxygenase and hydroxylamine oxidoreductase activities, and can convert NH in water 4+ Catalytic conversion to NO 2- . The Kurthia has high hydrolase activity, can hydrolyze and destroy the structure of tetracycline, promote the degradation of the tetracycline, and remove the inhibition effect of residual antibiotics in water on other microorganisms.
In the secondary biofilter, aeromonas has activity of nitrite reductase, the enzyme is psychrophilic enzyme, and NO in water can still be absorbed under the condition of low temperature 2- Catalytic reduction to N 2 。
In addition, in the secondary biofilter, zinc acetate is added to create good biochemical reaction conditions for aeromonas and nitrite reductase generated by the aeromonas, so that the nitrogen can be removed by the treatment system under the low-temperature condition. Zinc ions in the zinc acetate can be used as a cofactor of the nitrite reductase to improve the enzyme catalysis reaction efficiency of the nitrite reductase, and acetate ions in the zinc acetate can be used as a carbon source of the aeromonas, so that better nutrition is provided for the growth and planting of the aeromonas.
The invention realizes the removal of residual antibiotics and total nitrogen in a low-temperature environment by arranging two stages of biological treatment devices, namely a first-stage biofilm reactor and a second-stage biofilter, respectively inoculating different bacterial systems and carrying out different functional division.
The invention is further described with reference to specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The test materials and reagents used in the following examples are commercially available unless otherwise specified.
Example 1
The denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient broth agar culture medium, culturing to logarithmic growth phase, mixing 3 kinds of bacteria liquid, and controlling the amount of Sphingomonas in the mixed bacteria liquid to 10 9 The amount of CFU/mL and Pandora is 10 6 The number of CFU/mL and Kurthia sp is 10 8 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of pandora and 7 parts by mass of kurthella.
Inoculating aeromonas to an RS culture medium, culturing until the logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 9 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 55 percent of the effective volume of the primary biofilm reactor, the rest volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics, and 1g/L of aerobic activated sludge is inoculated. The primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 25%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 1.5mg/L, the field planting culture is carried out for 48h, and the primary biofilm reactor is started;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 40% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. Filling zeolite filter materials into the secondary biological filter, wherein the filling rate of the zeolite filter materials is 20%, controlling the dissolved oxygen concentration in the secondary biological filter to be 1.5mg/L, planting and culturing for 72h, and finishing the starting of the secondary biological filter; wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 6.8, and the concentration of dissolved oxygen in the reactor is 3.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 7.8, the dissolved oxygen concentration in the filter is 2.8mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 150mg/L.
After the denitrification treatment method of the embodiment is used for stable operation treatment, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 8.3mg/L, and the total nitrogen removal rate is 91.5%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the tetracycline content in the outlet water is 1.36mg/L, and the tetracycline removal rate in the water is 91.4%.
Example 2
The basic contents of this embodiment are the same as embodiment 1, except that: the denitrification treatment method for the residual antibiotic-containing polluted water body in the low-temperature environment comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient broth agar culture medium, culturing to logarithmic growth phase, mixing 3 kinds of bacteria liquid, and controlling the amount of Sphingomonas in the mixed bacteria liquid to 10 10 The number of the bacteria is 10 in CFU/mL and Pandora 7 CFU/mL, the number of Kurthia sp is 10 9 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of pandora and 7 parts by mass of kurthella.
Inoculating aeromonas to an RS culture medium, culturing until the logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 10 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 65 percent of the effective volume of the primary biofilm reactor, the rest volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics, and 1g/L of aerobic activated sludge is inoculated. The primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 35%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 2.5mg/L, field planting culture is carried out for 72h, and the primary biofilm reactor is started;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 55% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. Filling a zeolite filter material into the secondary biological filter, wherein the filling rate of the zeolite filter material is 25%, controlling the dissolved oxygen concentration in the secondary biological filter to be 2.5mg/L, planting and culturing for 84h, and finishing the start of the secondary biological filter; wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/4 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 7.5, and the concentration of dissolved oxygen in the reactor is 4.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 8.5, the dissolved oxygen concentration in the filter is 3.5mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 300mg/L.
After the denitrification treatment method of the embodiment is used for stable operation treatment, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 5.6mg/L, and the total nitrogen removal rate is 94.3%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the tetracycline content in the outlet water is 0.68mg/L, and the tetracycline removal rate in the water is 95.7%.
Comparative example 1
The comparative example is basically the same as example 1 except that: and S30, in the process of operating the treatment device at low temperature, zinc acetate is not added into the secondary biofilter.
The denitrification treatment method of the comparative example specifically comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient gravy agar culture medium, culturing to logarithmic growth phase, mixing 3 bacterial solutions, and controlling the amount of Sphingomonas in the mixed bacterial solution to 10 10 The number of the bacteria is 10 in CFU/mL and Pandora 7 The number of CFU/mL and Kurthia sp is 10 9 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of panduraemia and 7 parts by mass of Kurthia sp.
Inoculating aeromonas into RS culture medium, culturing to logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 10 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 65% of the effective volume of the primary biofilm reactor, the residual volume is supplemented and filled with high-nitrogen aquaculture wastewater containing antibiotics, and inoculating 1g/L of aerobic activated sludge. The primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 35%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 2.5mg/L, field planting culture is carried out for 72h, and the primary biofilm reactor is started;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 55% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. Filling a zeolite filter material into the secondary biological filter, wherein the filling rate of the zeolite filter material is 25%, controlling the dissolved oxygen concentration in the secondary biological filter to be 2.5mg/L, planting and culturing for 84h, and finishing the start of the secondary biological filter; wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/4 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 7.5, and the concentration of dissolved oxygen in the reactor is 4.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biofilter, the pH of the secondary biofilter is controlled to be 8.5, and the dissolved oxygen concentration in the biofilter is 3.5mg/L.
After the stable operation treatment of the denitrification treatment method of the comparative example, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 21.2mg/L, and the total nitrogen removal rate is 78.4%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the tetracycline content in the outlet water is 2.61mg/L, the removal rate of the tetracycline in the water is 83.5 percent, and the removal effect on the antibiotic and the total nitrogen is obviously lower than that of the embodiment 1-2.
Comparative example 2
The comparative example is basically the same as example 1 except that: the strains in the first microbial inoculum and the second microbial inoculum are different in quantity.
The denitrification treatment method of the comparative example specifically comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient gravy agar culture medium, culturing to logarithmic growth phase, mixing 3 bacterial solutions, and controlling the amount of Sphingomonas in the mixed bacterial solution to 10 5 The amount of CFU/mL and Pandora is 10 8 The number of CFU/mL and Kurthia sp is 10 7 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of panduraemia and 7 parts by mass of Kurthia sp.
Inoculating aeromonas into RS culture medium, culturing to logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 7 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 55% of the effective volume of the primary biofilm reactor, the residual volume is supplemented and filled with high-nitrogen aquaculture wastewater containing antibiotics, and inoculating 1g/L of aerobic activated sludge. The primary biofilm reactor is filled with a polypropylene combined filler, the filling rate of the polypropylene combined filler is 25%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 1.5mg/L, field planting culture is carried out for 48 hours, and the primary biofilm reactor is started;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 40% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. Filling a zeolite filter material into the secondary biological filter, wherein the filling rate of the zeolite filter material is 20%, controlling the dissolved oxygen concentration in the secondary biological filter to be 1.5mg/L, planting and culturing for 72h, and finishing the starting of the secondary biological filter; wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 6.8, and the concentration of dissolved oxygen in the reactor is 3.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 7.8, the dissolved oxygen concentration in the filter is 2.8mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 150mg/L.
After the stable operation treatment of the denitrification treatment method of the comparative example, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 26.8mg/L, and the total nitrogen removal rate is 72.7 percent; the concentration of the tetracycline in the inlet water is 15.8mg/L, the tetracycline content in the outlet water is 2.39mg/L, the removal rate of the tetracycline in the water is 84.9 percent, and the removal effect on the antibiotic and the total nitrogen is obviously lower than that of the embodiment 1-2.
Comparative example 3
The basic contents of this comparative example are the same as example 1, except that: the inoculation sequence of the first microbial inoculum and the second microbial inoculum is different.
The denitrification treatment method of the comparative example specifically comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient broth agar culture medium, culturing to logarithmic growth phase, mixing 3 kinds of bacteria liquid, and controlling the amount of Sphingomonas in the mixed bacteria liquid to 10 9 The number of the bacteria is 10 in CFU/mL and Pandora 6 The number of CFU/mL and Kurthia sp is 10 8 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of panduraemia and 7 parts by mass of Kurthia sp.
Inoculating Aeromonas onto RS culture medium, culturing until logarithmic growth phase, and controllingThe number of Aeromonas in the culture broth was 10 9 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a second microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the second microbial inoculum is 55% of the effective volume of the primary biofilm reactor, the residual volume is supplemented and filled with high-nitrogen aquaculture wastewater containing antibiotics, and inoculating 1g/L of aerobic activated sludge. The primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 25%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 1.5mg/L, the field planting culture is carried out for 48h, and the primary biofilm reactor is started;
inoculating a first microbial inoculum into the secondary biological filter, wherein the inoculation volume of the first microbial inoculum is 40% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. And zeolite filter materials are filled in the secondary biological filter, the filling rate of the zeolite filter materials is 20%, the dissolved oxygen concentration in the secondary biological filter is controlled to be 1.5mg/L, the planting culture is carried out for 72h, and the secondary biological filter is started.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 6.8, and the concentration of dissolved oxygen in the reactor is 3.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 7.8, the dissolved oxygen concentration in the filter is 2.8mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 150mg/L.
After the denitrification treatment method of the comparative example is used for stable operation treatment, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 36.7mg/L, and the total nitrogen removal rate is 62.6%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the tetracycline content in the outlet water is 3.10mg/L, the removal rate of the tetracycline in the water is 80.4 percent, and the removal effect on the antibiotic and the total nitrogen is obviously lower than that of the embodiment 1-2.
Comparative example 4
The basic contents of this comparative example are the same as example 1, except that: the step S20 differs in the activation condition for activating the processing device.
The denitrification treatment method of the comparative example specifically comprises the following steps:
s10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient broth agar culture medium, culturing to logarithmic growth phase, mixing 3 kinds of bacteria liquid, and controlling the amount of Sphingomonas in the mixed bacteria liquid to 10 9 The amount of CFU/mL and Pandora is 10 6 CFU/mL, the number of Kurthia sp is 10 8 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of pandora and 7 parts by mass of kurthella.
Inoculating aeromonas to an RS culture medium, culturing until the logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 9 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 40% of the effective volume of the primary biofilm reactor, the rest volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics, and 1g/L of aerobic activated sludge is inoculated. The primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 15%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 3.0mg/L, planting culture is carried out for 24h, and the primary biofilm reactor is started;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 30% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. Filling a zeolite filter material into the secondary biological filter, wherein the filling rate of the zeolite filter material is 30%, controlling the dissolved oxygen concentration in the secondary biological filter to be 1.0mg/L, planting and culturing for 36h, and finishing the start of the secondary biological filter; wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 6.8, and the concentration of dissolved oxygen in the reactor is 3.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 7.8, the dissolved oxygen concentration in the filter is 2.8mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 150mg/L.
After the stable operation treatment of the denitrification treatment method of the comparative example, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 39.8mg/L, and the total nitrogen removal rate is 59.4%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the content of the tetracycline in the outlet water is 3.38mg/L, the removal rate of the tetracycline in the water is 78.6 percent, and the removal effect on the antibiotic and the total nitrogen is obviously lower than that of the embodiment 1-2.
Comparative example 5
The basic contents of this comparative example are the same as example 1, except that: the operation conditions of the low-temperature operation processing device in step S30 are different.
S10, strain propagation culture
Respectively inoculating Sphingomonas, pandora and Kurthia to nutrient gravy agar culture medium, culturing to logarithmic growth phase, mixing 3 bacterial solutions, and controlling the amount of Sphingomonas in the mixed bacterial solution to be 10 9 The amount of CFU/mL and Pandora is 10 6 The number of CFU/mL and Kurthia sp is 10 8 CFU/mL. The mixed fermentation liquor is used as a first microbial inoculum; wherein the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of pandora and 7 parts by mass of kurthella.
Inoculating aeromonas into RS culture medium, culturing to logarithmic growth phase, and controlling the number of aeromonas in the culture solution to be 10 9 CFU/mL. The fermentation liquor is used as a second microbial inoculum.
S20, starting processing device
Inoculating a first microbial inoculum into the primary biofilm reactor, wherein the inoculation volume of the first microbial inoculum is 55% of the effective volume of the primary biofilm reactor, the residual volume is supplemented and filled with high-nitrogen aquaculture wastewater containing antibiotics, and inoculating 1g/L of aerobic activated sludge. The primary biofilm reactor is filled with a polypropylene combined filler, the filling rate of the polypropylene combined filler is 25%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 1.5mg/L, field planting culture is carried out for 48 hours, and the primary biofilm reactor is started;
inoculating a second microbial inoculum into the secondary biological filter, wherein the inoculation volume of the second microbial inoculum is 40% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. Filling zeolite filter materials into the secondary biological filter, wherein the filling rate of the zeolite filter materials is 20%, controlling the dissolved oxygen concentration in the secondary biological filter to be 1.5mg/L, planting and culturing for 72h, and finishing the starting of the secondary biological filter; wherein the mass ratio of the first microbial inoculum to the second microbial inoculum is 1.
S30, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH value of the primary biomembrane reactor is controlled to be 8.2, and the concentration of dissolved oxygen in the reactor is 1.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 6.8, the dissolved oxygen concentration in the filter is 2.0mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 100mg/L.
After the stable operation treatment of the denitrification treatment method of the comparative example, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 46.2mg/L, and the total nitrogen removal rate is 52.9%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the content of the tetracycline in the outlet water is 2.78mg/L, the removal rate of the tetracycline in the water is 82.4 percent, and the removal effect on the antibiotic and the total nitrogen is obviously lower than that of the embodiment 1-2.
Comparative example 6
The comparative example is a traditional denitrification treatment method of activated sludge, and no microbial inoculum is added. The denitrification treatment method of the comparative example specifically comprises the following steps:
s10, starting processing device
Inoculating activated sludge into a primary biomembrane reactor, wherein the inoculation volume of the activated sludge is 55 percent of the effective volume of the primary biomembrane reactor, the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics, and inoculating aerobic activated sludge of 1g/L. The primary biofilm reactor is filled with a polypropylene combined filler, the filling rate of the polypropylene combined filler is 25%, the dissolved oxygen concentration in the primary biofilm reactor is controlled to be 1.5mg/L, field planting culture is carried out for 48 hours, and the primary biofilm reactor is started;
inoculating activated sludge into the secondary biological filter, wherein the inoculation volume of the activated sludge is 40% of the effective volume of the secondary biological filter, and the residual volume is supplemented and filled with high-nitrogen culture wastewater containing antibiotics. And filling a zeolite filter material into the secondary biological filter, wherein the filling rate of the zeolite filter material is 20%, the dissolved oxygen concentration in the secondary biological filter is controlled to be 1.5mg/L, the planting culture is carried out for 72h, and the secondary biological filter is started.
S20, low-temperature operation processing device
After the primary biofilm reactor and the secondary biofilm filter are started, high-nitrogen wastewater containing antibiotics sequentially passes through the primary biofilm reactor and the secondary biofilm filter, the operation temperature is 5 ℃, and the inflow volume of the wastewater is controlled to be 1/3 of the total volume of the primary biofilm reactor and the secondary biofilm filter. The specific operation process of the primary biofilm reactor and the secondary biofilm filter is as follows: high-nitrogen wastewater containing antibiotics passes through a primary biomembrane reactor, the pH of the primary biomembrane reactor is controlled to be 6.8, and the concentration of dissolved oxygen in the reactor is 3.5mg/L; and then, the wastewater treated by the primary biofilm reactor passes through a secondary biological filter, the pH of the secondary biological filter is controlled to be 7.8, the dissolved oxygen concentration in the filter is 2.8mg/L, and zinc acetate is added into the secondary biological filter to maintain the zinc ion concentration in the wastewater to be 150mg/L.
After the stable operation treatment of the denitrification treatment method of the comparative example, the total nitrogen content of inlet water is 98mg/L, the total nitrogen content of outlet water is 73.6mg/L, and the total nitrogen removal rate is 24.9%; the concentration of the tetracycline in the inlet water is 15.8mg/L, the tetracycline content in the outlet water is 10.30mg/L, and the tetracycline removal rate in the water is 34.8%. Compared with the traditional denitrification treatment method for activated sludge, the embodiment 1-2 of the invention has obviously improved removal effect on antibiotics and total nitrogen.
The invention and its embodiments have been described above schematically, the description is not restrictive, the data used are only one of the embodiments of the invention, and the actual data combination is not limited to this. Therefore, if the person skilled in the art receives the teaching, the embodiments and examples similar to the above technical solutions shall not be designed in an inventive manner without departing from the spirit of the present invention, and shall fall within the protection scope of the present invention.
Claims (10)
1. A denitrifier for a residual antibiotic-containing polluted water body in a low-temperature environment is characterized in that: the microbial preparation comprises a first microbial agent and a second microbial agent, wherein the first microbial agent consists of sphingomonas, pandalata and Kurthia, the second microbial agent is aeromonas, and the mass ratio of the first microbial agent to the second microbial agent is 1: (2.5-3).
2. The denitrifying agent for the low-temperature environment polluted water body containing residual antibiotics according to claim 1, wherein: the first microbial inoculum comprises 10 parts by mass of sphingomonas, 1 part by mass of panduraemia and 7 parts by mass of Kurthia sp.
3. The denitrifying agent for low-temperature polluted water bodies containing residual antibiotics according to claim 2, wherein the denitrifying agent comprises the following components in percentage by weight: the number of sphingomonas in the first microbial inoculum is 10 9 ～10 10 The number of the bacteria is 10 in CFU/mL and Pandora 6 ～10 7 The number of CFU/mL and Kurthia sp is 10 8 ～10 9 CFU/mL。
4. A denitrification treatment method for a polluted water body containing residual antibiotics in a low-temperature environment is characterized by comprising the following steps: inoculating a first microbial inoculum into a primary biofilm reactor, inoculating a second microbial inoculum into a secondary biofilter, and respectively starting the primary biofilm reactor and the secondary biofilter; then sequentially carrying out denitrification treatment on the high-nitrogen wastewater containing the antibiotics by a primary biomembrane reactor and a secondary biological filter at the operation treatment temperature of below 10 ℃ to obtain a water body after denitrification treatment; wherein, in the process of treatment in the secondary biofilter, zinc acetate is added into the secondary biofilter, the first microbial inoculum consists of sphingomonas, pandora and Kurthia, and the second microbial inoculum is aeromonas.
5. The denitrification treatment method for the low-temperature-environment water body polluted by residual antibiotics according to claim 4, wherein the denitrification treatment method comprises the following steps: the inoculation volume of the first microbial inoculum is 55-65% of the effective volume of the primary biological membrane reactor, and the inoculation volume of the second microbial inoculum is 40-55% of the effective volume of the secondary biological filter.
6. The denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment according to the claim 4, characterized in that: in the process of starting the primary biofilm reactor and the secondary biofilm filter, the dissolved oxygen concentration in the primary biofilm reactor and the secondary biofilm filter is controlled to be 1.5-2.5 mg/L respectively.
7. The denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment according to the claim 4, characterized in that: inoculating the first microbial inoculum into a primary biofilm reactor, and planting and culturing for 48-72 h; and inoculating the second microbial inoculum into a secondary biological filter, and planting and culturing for 72-84 h.
8. The denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment according to the claim 4, characterized in that: in the process of denitrifying the high-nitrogen wastewater containing the antibiotics through a primary biofilm reactor and a secondary biofilm filter in sequence, controlling the pH of the primary biofilm reactor to be 6.8-7.5 and controlling the concentration of dissolved oxygen in the reactor to be 3.5-4.5 mg/L; controlling the pH value of the secondary biological filter to be 7.8-8.5 and controlling the concentration of dissolved oxygen in the filter to be 2.8-3.5 mg/L.
9. The denitrification treatment method for the low-temperature-environment water body polluted by residual antibiotics according to claim 4, wherein the denitrification treatment method comprises the following steps: and in the process of treating the wastewater in the secondary biofilter, adding zinc acetate into the secondary biofilter to maintain the concentration of zinc ions in the wastewater to be 150-300 mg/L.
10. The denitrification treatment method for the polluted water body containing the residual antibiotics in the low-temperature environment according to any one of claims 4-9, wherein the denitrification treatment method comprises the following steps: the primary biofilm reactor is filled with polypropylene combined filler, the filling rate of the polypropylene combined filler is 25-35%, the polypropylene combined filler consists of a plastic ring and a fiber bundle, the plastic ring is made of polypropylene materials, and the fiber bundle is made of hydroformylation fiber materials; and a zeolite filter material is filled in the secondary biological filter, and the filling rate of the zeolite filter material is 20-25%.
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