CN101955885B - High-efficiency denitrification mixed bacterial agent and application thereof - Google Patents
High-efficiency denitrification mixed bacterial agent and application thereof Download PDFInfo
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- CN101955885B CN101955885B CN2010101074536A CN201010107453A CN101955885B CN 101955885 B CN101955885 B CN 101955885B CN 2010101074536 A CN2010101074536 A CN 2010101074536A CN 201010107453 A CN201010107453 A CN 201010107453A CN 101955885 B CN101955885 B CN 101955885B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a high-efficiency denitrification mixed bacterial agent and application thereof, and a novel bacterial strain capable of efficiently denitrifying obtained by screening. The high-efficiency denitrification mixed bacterial agent consists of CCTCC No:M 2010001 and nitrosobacteria CCTCC No:M 2010002. The invention mainly has the advantages of providing a high-efficiency denitrification mixed bacterial agent, greatly improving the efficiency for treating sewage, not causing secondary pollution, and having significance for improving ecological environment.
Description
(1) technical field
The object of the invention provides a kind of mix bacterium agent and application thereof of efficient denitrification, but and the new bacterial strain of the efficient denitrification of screening acquisition.
(2) background technology
Along with the continuous development with industrial or agricultural of expanding day by day of urban population, the water environment pollution accident takes place frequently, serious harm the health and even the life of people, animal.Many lakes and reservoir cause body eutrophication because of the discharging of nitrogen, phosphorus, and serious threat is to the Human's production life and the eubiosis.And ammonia nitrogen is one of principal element that causes body eutrophication.In order to satisfy the public to the improving constantly of environmental quality requirement, country has worked out more and more strict emission control standards to nitrogen, and research and development are economical, denitrogenate emphasis and the focus that treatment technology has become water pollution control engineering area research efficiently.Though have many methods can both remove ammonia effectively, r-o-, distillation, soil irrigation arranged like physical method; Chemical method has ion exchange method, ammonia stripping, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatment, catalytic pyrolysis; Biological method has nitrated and algal culture, but it is applied to the processing of trade effluent, must have convenient in application, handling property and stablize, is adapted to waste water quality and advantage such as both economical.
That handles at present that the method for ammonia nitrogen mainly adopts denitrogenates process air stripping, breakpoint chlorination and biological nitration and denitrification etc.
1, air stripping
Under alkaline condition (pH＞10.5), the ammonia nitrogen in the waste water is mainly with NH
3Form exist.Let waste water fully contact, then volatile NH in the water with air
3To shift to gas phase by liquid phase, thereby remove the ammonia nitrogen in the water.Wooden or the plastic slat filler of filling in the stripping tower, airflow is got into by the bottom of tower, and waste water then drops down onto water collecting basin at the bottom of the tower by cat head.The principal element that influences ammonia stripping effect has: (1) pH value generally is increased to 10.8～11.5 with the pH value; (2) temperature, the solubleness of ammonia increased when water temperature reduced, and stripping efficient reduces; (3) hydraulic load, general hydraulic load is 2.5～5m
3/ m
2.h; (4) GWR for certain tower height, increases air flow quantity, can improve the ammonia clearance; But along with air flow quantity increases, pressure drop also increases, so air flow quantity has a limit value.Generally, gas/water is than desirable 2500～5000 (m
3/ m
2); (5) filler configuration and height, owing to dabble repeatedly and form the key that water droplet is the ammonia stripping, so the shape of filler, size, spacing, arrangement mode are enough all influential to the stripping effect.Generally, filler spacing 40～50mm, packed height is 6～7.5m.If increase the filler spacing, then need bigger packed height; (6) fouling control, filler fouling (CaCO
3) the special processing efficiency that reduces stripping tower.The measure of control fouling has: with high pressure water washing dirt layer; In water inlet, add Scale inhibitors: adopt not conform to or contain less CO
2Air stripping (absorb removing ammonia like tail gas recycles); Adopt less scaling plastic filler replacing wood etc.
Air stripping method removes ammonia, and clearance can reach 60～95%, and flow process is simple, and treatment effect is stable, and capital cost and running cost are lower, but treatment of high concentration is closed ammonia waste water.But stripping efficient was low when temperature was low, fill out section's fouling and often seriously disturb operation, and the ammonia that stripping goes out produces secondary pollution to environment.
2, breakpoint chlorination
Add excess chlorine or Youxiaolin, make that the ammonia complete oxidation is N in the waste water
2Method, be called break point chlorination, its reaction can be expressed as: NH
4 ++ 1.5HOCl → 0.5N
2+ 1.5H
2O+2.5H
++ 1.5Cl
-Can know that by reaction formula the theory that arrives break needs chlorine (Cl
2) amount is for 7.6kg/kg (NH
3-N), and actual chlorine demand is at 8～10kg/kg (NH
3-N).React in pH=6～7, then dosage can be minimum.Be generally 0.5～2h duration of contact.Strict control pH value and chlorine-throwed quantity can reduce generating deleterious chloramines in the reaction (like NCl
3) and the chlorinatedorganic break point chlorination ammonia-N removal rate is reached 90～100%, treatment effect is stable, not influenced by water temperature, capital cost is not high yet.But its working cost is high; Residual chlorine and chlorinatedorganic must carry out aftertreatment.
In the denitrification process that adopts at present, physics such as air stripping and breakpoint chlorination and chemical method are owing to existing the running cost height, environment being caused secondary pollution problems, and practical application receives certain limitation.
3, biological nitration and denitrification
Biological nitration through the effect of nitrite bacteria and nitrate salt bacterium, is oxidized to the process of nitrite nitrogen and nitrate nitrogen with ammonia nitrogen under aerobic condition, be called the biological nitration effect.Under anoxia condition, because the effect of facultative denitrifier (denitrifying bacteria), with NO
2--N and NO
3--N is reduced into N
2Process, be called denitrification.
The principal element that influences nitrifying process has: (1) pH value, when the pH value is 8.0～8.4 (20 ℃), nitrification is fastest.(2) temperature, when temperature was high, nitrated speed was fast.(3) sludge retention time, in order to keep a certain amount of nitrifying bacteria community in the pond, sludge retention time must be greater than the minimum generation time of nitrifier; (4) dissolved oxygen generally carries out nitratedly in the activated sludge process aeration tank, and dissolved oxygen should remain on more than 2～3mg/L; (5) BOD loading, nitrated for will fully carrying out, BOD
5Load should maintain 0.3kg (BOD
5)/kg (SS) is below the .d.Influence denitrifying principal element: (1) temperature, temperature wants big to denitrifying other biological wastewater treatment process of influence comparison.Generally, be advisable to keep 20～40 ℃.If in temperature low excessively winter, can take to increase sludge retention time, reduce measure such as load, to keep good denitrification effect; (2) pH value, the pH value of denitrification process is controlled at 7.0～8.0; (3) dissolved oxygen, generally dissolved oxygen should be controlled at (activated sludge process) below the 0.5mg/L or (biomembrance process) below the 1mg/L in denitrification reactor; (4) organic carbon source contains enough organic carbon sources, BOD in waste water
5During/TN＞(3～5), can need not additional carbon.When the contained carbon of waste water, when the nitrogen ratio is lower than this ratio, just need add organic carbon in addition.Add organic carbon and adopt methyl alcohol more.
Biological process is compared with chemical substance has following advantage:
1) every kind of chemical article all is the very strong product of specific aim, when running into other chemical substances, just might lose efficacy, and biotechnological formulation has spectrum property to the removal of pollutent.
2) chemical products can temporarily be eliminated some objectionable impurities and mask malodors, lacks the generation that can not stop objectionable impurities.
3) use chemical products after, have residually in the water body, possibly cause secondary pollution.The contained natural microbial of biotechnological formulation does not contain pathogenic bacterium and pathogenic agent, and these mikrobes are under the katalysis of enzyme; With the organic nutrient substance in the sewage is food; After sewage was purified, these mikrobes can reduce with the reduction of pollutent gradually, until extinction.
4) nontoxic, non-corrosiveness, easy to use, need not add equipment or engineering basically, save fund input.
(3) summary of the invention
The object of the invention provides a kind of mix bacterium agent and application thereof of efficient denitrification, but and the new bacterial strain of the efficient denitrification of screening acquisition.
The technical scheme that the present invention adopts is:
A kind of mix bacterium agent of efficient denitrification is made up of nitrifier CCTCC No:M 2010001 and nitrococcus CCTCC No:M 2010002.Main points of the present invention are: the denitrogenation processing that nitrifier CCTCC No:M 2010001 and nitrococcus CCTCC No:M 2010002 is mixed for nitrogen-containing wastewater; Its blending ratio can be unrestricted; In fact, because influencing each other and restricting between the different microorganisms flora, under the nitrifier of in Sewage treatment systems, initially dropping into and the uncertain situation of nitrococcus ratio; Through acclimation shaking culture, two kinds of floras can reach certain balance in the final treatment system.
Preferably, the ratio of the biomass of nitrifier CCTCC No:M 2010001 and nitrococcus CCTCC No:M 2010002 is 0.5～2: 1 in the said mix bacterium agent.
More preferred, the ratio of the biomass of nitrifier CCTCC No:M 2010001 and nitrococcus CCTCC No:M 2010002 is 1: 1 in the said mix bacterium agent.
Mix bacterium agent of the present invention is from the dominant bacteria of occurring in nature screening or the high-efficiency strain of producing through assortment of genes technology, wherein contains the compound microbial of various elite types, when specifically using; Also can add zymin pointedly; Nutritive substance and mineral salt put it in useless, the sewage, and intrasystem microbe population is increased; Improve the efficient of WWT, and the use of biotechnological formulation can not cause secondary pollution.
The invention still further relates to the application of described mix bacterium agent in handling nitrogen-containing wastewater.
Concrete, said being applied as: said mix bacterium agent is seeded to the anaerobic pond of sewage works, move 5～10 days, obtain the active sludge after the domestication, nitrogen-containing wastewater is carried out aerobic treatment with the active sludge after taming.
The bacterial strain that the present invention relates to is the new bacterial strain that screens:
Nitrobacter (Nitrobacter sp.) Sundawater X-5 is preserved in Chinese typical culture collection center, the address: Chinese Wuhan Wuhan University, 430072, preservation date on January 8th, 2010, deposit number CCTCC No:M 2010001.
Nitrosification Pseudomonas (Nitrosomonas sp.) Sundawater X-6 is preserved in Chinese typical culture collection center, the address: Chinese Wuhan Wuhan University, 430072, preservation date on January 8th, 2010, deposit number CCTCC No:M 2010002.
Efficient denitrification mix bacterium agent of the present invention has following certain benefits: (1) is rapidly from by recovering organism or inhibition impact load, hydraulic load excess or the burst nitrification tangleweed that loss of solids caused; (2) improve nitrification efficiency, or stably produce nitrification in the operational system midium or long term; (3) be provided for the nitrated inoculation that season starts, for inoculating in the large scale system factory; (4) reduce owing to nitrated hydrochlorate is assembled the chlorine consumption that causes; (5) system that SF is not enough drops to minimum to the influence of nitrification; (6) improvement system is to be used as the biogenic amine clearing device in the lower sewage of BOD content; (7) the approximate tolerance of salinity: salinity was similar to the tolerance of salinity 100% at 0% o'clock; Salinity was similar to the tolerance of salinity 92% at 1.5% o'clock; Salinity was similar to the tolerance of salinity 44% at 2.5% o'clock; Salinity was similar to the tolerance of salinity 11% at 3.5% o'clock.
And following characteristic is arranged: the nitrated mikrobe that exists with liquid form is contained in (1);
(2) specification is based on the rate of oxidation to ammonia nitrogen;
(3) can concentrate be easy to refrigeration;
(4) do not contain dhdps enzyme, phenotype promoting agent or solvent;
(5) can be attached on the kinds of surface that helps the microbial film form.
Beneficial effect of the present invention is mainly reflected in: a kind of mix bacterium agent of efficient denitrification is provided, can have improved the efficient of WWT greatly, and the use of this microbial inoculum can not cause secondary pollution, and for the improvement of ecotope, significant.
(4) embodiment
Below in conjunction with specific embodiment the present invention is described further, but protection scope of the present invention is not limited in this:
Embodiment 1:
Denitrifying bacteria culturing process of the present invention:
The substratum proportioning is: 2% glucose (w/v), and 1.5% Carnis Bovis seu Bubali cream (w/v), 1.5% peptone (w/v), 0.058% sal epsom (w/v), 0.025% manganous sulfate (w/v), 0.22% sodium acetate (w/v), 0.2% potassium hydrogenphosphate (w/v), solvent are water.Above-mentioned per-cent is mass and size per-cent, contains this component 1g in certain concentration of component 1% expression 100mL substratum.
Fermentation condition: initial pH value is 5.8, inoculates CCTCC No:M 2010001 and CCTCC No:M 2010002 respectively, keeps 2% glucose (w/v) and 0.22% sodium succinate (w/v) during the fermentation.Under 30 ℃, mixing speed is 400r/min, and air flow is 1vvm (a 7L jar), cultivates 22h, and the bacterium number can reach 1.17 * 10 in the fermented liquid
10Cfu/mL.
The sludge acclimatization process:
The microbial fermentation solution that obtains of fermentation is by after 1: 1 volume mixture, adds anaerobic pond according to the dosage of 5% (v/v) (the microbial fermentation mixed solution that adds 5ml in the treatment sewage of every 100ml), keeps the active sludge after obtaining taming 7 days.
Embodiment 2:
Huzhou City weighs million village's ecological model village sewages, and raw water quality is BOD5 (BOD Biology Oxygen Demmand on the five) 250mg/L, COD (chemical oxygen demand; Chemicaloxygen demand) 400mg/L; Total nitrogen 30mg/L, ammonia nitrogen 10mg/L respectively gets 1000ml, places Glass Containers; Carry out the aerobic stage processing with this village's sewage works mud and by the mud after the domestication of embodiment 1 method respectively, aeration rate is 1vvm.Behind the reaction 18h, the water sample water quality BOD5 20mg/L of raw waste water station sludge treatment, COD 40mg/L, total nitrogen 20mg/L, ammonia nitrogen 6mg/L; The water sample water quality of utilizing this patent acclimation sludge to handle is BOD5 6mg/L, COD 20mg/L, total nitrogen 7mg/L, ammonia nitrogen 3mg/L; Adopt mix bacterium agent of the present invention, overall efficiency improves about 50%.
Embodiment 3:
Huzhou City weighs million village's ecological model village sewages under special circumstances; Raw water quality is BOD5550mg/L, COD 1000mg/L, total nitrogen 60mg/L; Ammonia nitrogen 25mg/L respectively gets 1000ml; Place Glass Containers, carry out aerobic stage with this village's sewage works mud and the mud of taming by embodiment 1 method respectively and handle, aeration rate is 1vvm.Behind the reaction 24h, the water sample water quality BOD5 50mg/L of raw waste water station sludge treatment, COD 100mg/L, total nitrogen 30mg/L, ammonia nitrogen 15mg/L; The water sample water quality of utilizing this patent acclimation sludge to handle is BOD5 8mg/L, COD 30mg/L, total nitrogen 8mg/L, ammonia nitrogen 4mg/L; Impact resistance has obtained checking.
Embodiment 4:
Wuxi City Lu Zhuan domestic sewage factory; Raw water quality is BOD5 150mg/L, COD 300mg/L, total nitrogen 32mg/L; Ammonia nitrogen 12mg/L respectively gets 1000ml; Place Glass Containers, carry out aerobic stage with this sludge of sewage treatment plant and the mud of taming by embodiment 1 method respectively and handle, aeration rate is 1vvm.Behind the reaction 18h, the water sample water quality BOD515mg/L of raw waste water station sludge treatment, COD 20mg/L, total nitrogen 16mg/L, ammonia nitrogen 5mg/L; The water sample water quality of utilizing this patent acclimation sludge to handle is BOD5 5mg/L, COD 17mg/L, total nitrogen 4mg/L, ammonia nitrogen 2mg/L; Adopt mix bacterium agent of the present invention, overall efficiency improves about 50%.
Claims (4)
1. the mix bacterium agent of an efficient denitrification is that Nitrobacter (Nitrobacter sp.) the Sundawater X-5 of CCTCC No:M 2010001 and nitrosification Pseudomonas (Nitrosomonas sp.) Sundawater X-6 that deposit number is CCTCC No:M 2010002 form by deposit number.
2. the application of mix bacterium agent as claimed in claim 1 in handling nitrogen-containing wastewater.
3. application as claimed in claim 2; It is characterized in that said being applied as: the anaerobic pond that said mix bacterium agent is seeded to sewage works; Move 5～10 days, the active sludge after obtaining to tame carries out aerobic treatment with the active sludge after the domestication to nitrogen-containing wastewater.
4. Nitrobacter (Nitrobacter sp.) Sundawater X-5 is preserved in Chinese typical culture collection center, the address: Chinese Wuhan Wuhan University, 430072, preservation date on January 8th, 2010, deposit number CCTCC No:M 2010001.
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| CN102864107B (en) * | 2012-09-26 | 2014-08-13 | 朱文霞 | Microbial preparation for rehabilitating phenyl amine polluted water body |
| CN102888374B (en) * | 2012-09-26 | 2014-08-13 | 闫家怡 | Biological agent for processing industrial sewage and preparation method and application |
| CN102864109B (en) * | 2012-09-26 | 2014-02-05 | 李丽萍 | Preparation for trinitrotoluene-contained sewage treatment and using method of preparation |
| CN103086502A (en) * | 2013-01-08 | 2013-05-08 | 北京工业大学 | Rapid styrene butadiene rubber (SBR) nitrosoation recovery method |
| CN103102009A (en) * | 2013-02-25 | 2013-05-15 | 北京工业大学 | Method for induced recovery of SBR nitrosation |
| CN103320610B (en) * | 2013-07-03 | 2014-08-06 | 山东黄金矿业(沂南)有限公司 | Sorting technique for valuable elements associated with gold |
| CN103382057B (en) * | 2013-07-28 | 2014-06-25 | 华盛江泉集团有限公司 | Method for treating cultivation wastewater |
| CN105543150B (en) * | 2016-02-22 | 2020-03-13 | 杭州富阳高博信息技术服务有限公司 | Bioremediation preparation for aniline polluted water |
| CN105505841B (en) * | 2016-02-24 | 2019-08-16 | 杭州富阳高博信息技术服务有限公司 | For handling the microbial bacterial agent of industrial wastewater pollution |
| CN107828773A (en) * | 2017-11-08 | 2018-03-23 | 常州文诺纺织品有限公司 | A kind of preparation method of the domestication agent of the activated sludge of brine waste processing |
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Application publication date: 20110126 Assignee: ZHEJIANG SUNDA ENVIRONMENTAL PROTECTION Co.,Ltd. Assignor: ZHEJIANG SUNDAWATER Co.,Ltd. Contract record no.: 2012330000476 Denomination of invention: High-efficiency denitrification mixed bacterial agent and application thereof Granted publication date: 20120704 License type: Exclusive License Record date: 20120827 |
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