CN102864107B - Microbial preparation for rehabilitating phenyl amine polluted water body - Google Patents
Microbial preparation for rehabilitating phenyl amine polluted water body Download PDFInfo
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- CN102864107B CN102864107B CN201210364480.0A CN201210364480A CN102864107B CN 102864107 B CN102864107 B CN 102864107B CN 201210364480 A CN201210364480 A CN 201210364480A CN 102864107 B CN102864107 B CN 102864107B
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Abstract
The invention relates to a microbial preparation for treating a phenyl amine polluted water body, and a preparation method and application thereof. The microbial preparation is prepared from the following raw materials in percentage by weight: 25% of Pseudomonas aeruginosa, 15% of nitrococcus, 15% of Bacillus subtilis, 15% of Bacillus foecalis alkaligenes, 10% of Saccharomyces cerevisiae, 10% of Rhodococcus, 5% of Bacillus megaterium and 5% of Aspergillus niger. According to the invention, the strains are matched with each other reasonably, and realize symbiotic coordination without mutual antagonism; and when the microbial preparation is thrown into a wastewater treatment system, nondegradable phenyl amine compounds can be favorably degraded. Thus, the microbial preparation is suitable for industrial sewage treatment, improves the quantity and quality of treated water, lowers the operating cost and promotes the standardized discharge.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of microbial preparation of processing amine polluted-water, and preparation method thereof.
Background technology
Along with day by day expanding and the development of industrial or agricultural of urban population, water environment pollution frequent accident, serious harm health and even the threat to life of people, animal.Industrial sewage composition is more complicated, and aniline is the industrial chemicals of widespread use, serious environment pollution and being detrimental to health, and there is carinogenicity.Because amino benzenes compounds is to biological toxicity, pay close attention to so be subject to people always.China lists the preferential priority pollutant of controlling in environment in amino benzenes compounds, and industrial water drainage is carried out to stringent regulations, has formulated the highest permissible discharge standard, and its concentration is 5mg/L.And drinking water standard is only 0.2mg/L.
The water body treating that amino benzenes compounds pollutes is difficulty comparatively, and domestic research is less, and up to now, most physics, chemical processes of adopting are processed, but these method processing costss are higher, and technical requirements is comparatively strict, is difficult to promote in practical application.
Bioremediation is to administer the effective ways of amino benzenes compounds polluted-water.It is the effect that utilizes microorganism, and amino benzenes compounds is decomposed.Because bioremediation is more much lower than physics, chemical process cost, non-secondary pollution again, and microorganism have again stronger can variability and adaptability, therefore it has become the Perfected process of processing amino benzenes compounds polluted-water.
Amino benzenes compounds degradation bacteria is very essential to the thorough degraded of amino benzenes compounds.The amino benzenes compounds in degradation property microbiological treatment trade effluent has been applied in many chemical plant, and obtain significant effect, but can't meet actual needs completely, quite a few industrial pollution enterprise of China would rather be punished to be also unwilling to invest and administer waste water, even if there is waste disposal plant operation also abnormal.Therefore, develop that a kind of construction investment is few, running cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Summary of the invention
The object of this invention is to provide a kind of effectively compound microbial inoculant for the treatment of amino benzenes polluted water and utilize high efficiency composition microbial inoculum to process the method for polluted-water.
Effectively compound microbial inoculant of the present invention, reasonable compatibility between each bacterial classification, symbiosis is coordinated, antagonism not mutually, its preparation method is easy, easy to implement the method, and it is easy and simple to handle, is beneficial to production.
The present invention adopts following technical scheme to realize:
Repair a microbial preparation for amino benzenes polluted water, the activeconstituents of said preparation comprises the raw material of following weight percent:
Pseudomonas aeruginosa 25%, nitrococcus 15%, subtilis 15%, Bacillus foecalis alkaligenes 15%, yeast saccharomyces cerevisiae 10%, rhodococcus 10%, bacillus megaterium 5%, aspergillus niger 5%.
In mentioned microorganism preparation:
Described Pseudomonas aeruginosa is preferably Pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC15442 (for example, referring to document Adaptation of Pseudomonas aeruginosa ATCC15442to didecyldimethylammonium bromide induces changes in membrane fatty acid composition and in resistance of cells, Journal of Applied Microbiology, 2001);
Described nitrococcus is preferably Nitrosomonas europaea (Nitrosomonas europaea) CCTCC No:M2010002 (CN101955885 openly uses);
Described subtilis is preferably subtilis (Bacillus subtilis) CGMCC No:0954 (CN1554744 openly uses);
Described Bacillus foecalis alkaligenes is that Bacillus foecalis alkaligenes (Alcaligenes faecalis) ATCC31555 is (for example, referring to document Structural studies of an extracellular polysaccharide (S-130) elaborated by Alcaligenes ATCC31555, Carbohydrate Research, 1986);
Described yeast saccharomyces cerevisiae is preferably yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CCTCC No:M208110 (CN101434911 openly uses);
Described rhodococcus is preferably prunosus red coccus (Rhodococcus rhodochrous) ATCC15906 (for example, referring to Cloning and Characterization of Benzoate Catabolic Genes in the Gram-Positive Polychlorinated Biphenyl DegraderRhodococcus sp.Strain RHA1, J.Bacteriol.November2001);
Described bacillus megaterium is preferably bacillus megaterium (Bacillus megatherium) CGMCC No:2267 (CN101215532 openly uses);
Described aspergillus niger is preferably aspergillus niger (Aspergillus niger) CCTCC No:M206034 (CN1924000 openly uses).
Bacterial classification of the present invention all can be from Chinese microorganism strain preservation management committee's common micro-organisms center (CGMCC), Chinese Typical Representative culture collection center (CCTCC) and US mode culture collection warehousing (ATCC) are bought and obtained.
Wherein each strain fermentating liquid preparation process is:
1, the independent enlarged culturing of various bacterial classifications:
The cultivation of A, subtilis or bacillus megaterium: first subtilis or bacillus megaterium test tube kind are seeded on beef-protein medium, 28-30 DEG C, making primary inclined plane cultivates, then be inoculated into and in triangular flask, do vibration secondary liquid culture, then proceed to liquid fermentation tank and do three grades of liquid culture, finally be inoculated into and on solid medium, make level Four and cultivate, reach 2.0-4.0 × 10 to viable count in product
8individual/gram.
The cultivation of B, rhodococcus: rhodococcus bacterial classification is seeded in respectively on nutrient broth medium (with reference to microbiology test textbook), 28-30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 2.0-5.0 × 10
8individual/gram.
The cultivation of C, yeast saccharomyces cerevisiae or aspergillus niger: first yeast saccharomyces cerevisiae or aspergillus niger are seeded on potato dextrose agar (PDA), 28-30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0 × 10
8individual/gram.
D, Pseudomonas aeruginosa cultivate or Bacillus foecalis alkaligenes: Pseudomonas aeruginosa or Bacillus foecalis alkaligenes are first on substratum, and 28-30 DEG C, makes primary inclined plane and cultivate, and then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0 × 10
8individual/gram, described medium component is: NH
4cl1.0g, CH
3cOONa3.5g, MgCl
20.1g, CaCl
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000mI, pH7.2.
The cultivation of E, nitrococcus: nitrococcus is first on substratum, and 28-30 DEG C, makes primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0 × 10
8individual/gram, described medium component is: 2% glucose (w/v), 1.5% extractum carnis (w/v), 1.5% peptone (w/v), 0.058% magnesium sulfate (w/v), 0.025% manganous sulfate (w/v), 0.22% sodium acetate (w/v), 0.2% dipotassium hydrogen phosphate (w/v), solvent is water;
By above Pseudomonas aeruginosa, nitrococcus, subtilis, Bacillus foecalis alkaligenes, yeast saccharomyces cerevisiae, rhodococcus, bacillus megaterium, aspergillus niger, the bacterium liquid of cultivating is mixed to get liquid bacterial agent according to mass ratio;
F, get aforesaid liquid microbial inoculum and carrier is uniformly mixed, preferably taking diatomite (40-80 order) as carrier, according to microbial inoculum: carrier is 3-4: 1 weight ratio is mixed.Dry: will mix material and be dried, drying temperature is 20-50 DEG C, dry rear water content is 20-30%; Inspection, packaging: by quality standard inspection, finished product is packed by weight, obtains solid preparation.
Note, in above-mentioned steps, strain expanded culture and the method for preparing solid preparation are not unique, those skilled in the art can select suitable substratum and enlarged culturing method according to general knowledge, make viable count reach 10
8individual/gram, and prepare the method preparation of microbial solid preparation according to routine.
Composite fungus agent of the present invention is by the various bacterial classifications that can form dominant microflora, be mixed with high-efficiency microorganism preparation, be added in Waste Water Treatment by a certain amount of, accelerate the degraded of microbe, to improve the biological treatment efficiency of system, ensure system stable operation.It contains the multiple microorganism that difficult degradation pollutent is had to good degradation capability, reasonable compatibility between each bacterial classification, and symbiosis is coordinated, antagonism not mutually, active high, biomass is large, and breeding is fast, add in Waste Water Treatment, amino benzenes polluted water is had to good degradation effect, be suitable for industrial sewage processing, can improve and process the water yield and water quality treatment, reduce working cost, promote qualified discharge.
Embodiment
Embodiment 1:
Repair a microbial preparation for amino benzenes polluted water, the activeconstituents of said preparation comprises the raw material of following weight percent:
Pseudomonas aeruginosa 25%, nitrococcus 15%, subtilis 15%, Bacillus foecalis alkaligenes 15%, yeast saccharomyces cerevisiae 10%, rhodococcus 10%, bacillus megaterium 5%, aspergillus niger 5%.
In mentioned microorganism preparation:
Described Pseudomonas aeruginosa is preferably Pseudomonas aeruginosa (Pseudomonasaeruginosa) ATCC15442;
Described nitrococcus is preferably Nitrosomonas europaea (Nitrosomonas europaea) CCTCC No:M2010002;
Described subtilis is preferably subtilis (Bacillus subtilis) CGMCC No:0954;
Described Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes (Alcaligenes faecalis) ATCC31555;
Described yeast saccharomyces cerevisiae is preferably yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CCTCC No:M208110;
Described rhodococcus is preferably prunosus red coccus (Rhodococcus rhodochrous) ATCC15906;
Described bacillus megaterium is preferably bacillus megaterium (Bacillus megatherium) CGMCC No:2267;
Described aspergillus niger is preferably aspergillus niger (Aspergillusniger) CCTCC No:M206034.
Bacterial classification of the present invention all can be from Chinese microorganism strain preservation management committee's common micro-organisms center (CGMCC) and Chinese Typical Representative culture collection center (CCTCC) and US mode culture collection warehousing (ATCC), Chinese Typical Representative culture collection center buy and obtain.
Wherein each strain fermentating liquid preparation process is:
1, the independent enlarged culturing of various bacterial classifications:
The cultivation of A, subtilis or bacillus megaterium: first subtilis or bacillus megaterium test tube kind are seeded on beef-protein medium, 28-30 DEG C, making primary inclined plane cultivates, then be inoculated into and in triangular flask, do vibration secondary liquid culture, then proceed to liquid fermentation tank and do three grades of liquid culture, finally be inoculated into and on solid medium, make level Four and cultivate, reach 2.0-4.0 × 10 to viable count in product
8individual/gram.
The cultivation of B, rhodococcus: rhodococcus bacterial classification is seeded in respectively on nutrient broth medium (with reference to microbiology test textbook), 28-30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 2.0-5.0 × 10
8individual/gram.
The cultivation of C, yeast saccharomyces cerevisiae or aspergillus niger: first yeast saccharomyces cerevisiae or aspergillus niger are seeded on potato dextrose agar (PDA), 28-30 DEG C, make primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0 × 10
8individual/gram.
D, Pseudomonas aeruginosa cultivate or Bacillus foecalis alkaligenes: Pseudomonas aeruginosa or Bacillus foecalis alkaligenes are first on substratum, and 28-30 DEG C, makes primary inclined plane and cultivate, and then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0 × 10
8individual/gram, described medium component is: NH
4cl1.0g, CH
3cOONa3.5g, MgCl
20.1g, CaCl
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000mI, pH7.2.
The cultivation of E, nitrococcus: nitrococcus is first on substratum, and 28-30 DEG C, makes primary inclined plane and cultivate, then secondary seed cultivation, mixing fermentation culture etc. to viable count in product reaches 1.0-5.0 × 10
8individual/gram, described medium component is: 2% glucose (w/v), 1.5% extractum carnis (w/v), 1.5% peptone (w/v), 0.058% magnesium sulfate (w/v), 0.025% manganous sulfate (w/v), 0.22% sodium acetate (w/v), 0.2% dipotassium hydrogen phosphate (w/v), solvent is water;
By above nitrococcus, subtilis, Bacillus foecalis alkaligenes, Pseudomonas aeruginosa, yeast saccharomyces cerevisiae, rhodococcus, bacillus megaterium, aspergillus niger, the bacterium liquid of cultivating is mixed to get liquid bacterial agent according to mass ratio;
F, get aforesaid liquid microbial inoculum and carrier is uniformly mixed, preferably taking diatomite (40-80 order) as carrier, according to microbial inoculum: carrier is 3-4: 1 weight ratio is mixed.Dry: will mix material and be dried, drying temperature is 20-50 DEG C, dry rear water content is 20-30%; Inspection, packaging: by quality standard inspection, finished product is packed by weight, obtains solid preparation.
Note, in above-mentioned steps, strain expanded culture and the method for preparing solid preparation are not unique, those skilled in the art can select suitable substratum and enlarged culturing method according to general knowledge, make viable count reach 10
8individual/gram, and prepare the method preparation of microbial solid preparation according to routine.
Embodiment 2
The using method of the microbial solid preparation that embodiment 1 prepares: solid preparation is put in the polluted-water of aniline, wherein the part by weight of solid preparation and water body is 1: 500, wherein the polluted-water of aniline is artificial preparation, aniline starting point concentration is 48.79mg/L, then shaking culture after 24 hours under normal temperature, the density loss of aniline is to 0.01mg/L, and degradation rate can reach 99.9%.
Embodiment 3
The microbial solid preparation that adopts embodiment 1 to prepare is processed certain chemical plant sewage, in sewage, COD concentration is 420mg/L, the concentration of dinitrotoluene (DNT)+trotyl is 120mg/L, concentration of aniline is 58mg/L, adds by every cubic metre of sewage 20 grams, the preparation that embodiment 1 makes at every turn, adds every day 1 time, add continuously after 10 days, the treatment effect detecting is as following table: effluent COD concentration is 20mg/L, and nitrobenzene concentration is below 1mg/L, and phenyl amines concentration is 0mg/L.
Although, above with general explanation and embodiment, this case being done to detailed explanation, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (2)
1. repair the microbial preparation of amino benzenes polluted water for one kind, the activeconstituents of said preparation comprises the raw material of following weight percent: Pseudomonas aeruginosa 25%, nitrococcus 15%, subtilis 15%, Bacillus foecalis alkaligenes 15%, yeast saccharomyces cerevisiae 10%, rhodococcus 10%, bacillus megaterium 5%, aspergillus niger 5%;
Described Pseudomonas aeruginosa is Pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC15442;
Described nitrococcus is Nitrosomonas europaea (Nitrosomonas europaea) CCTCC No:M2010002;
Described subtilis is subtilis (Bacillus subtilis) CGMCC No:0954;
Described Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes (Alcaligenes faecalis) ATCC31555;
Described yeast saccharomyces cerevisiae is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) CCTCC No:M208110;
Described rhodococcus is prunosus red coccus (Rhodococcus rhodochrous) ATCC15906;
Described bacillus megaterium is bacillus megaterium (Bacillus megatherium) CGMCC No:2267;
Described aspergillus niger is aspergillus niger (Aspergillus niger) CCTCC No:M206034.
2. the application of microbial preparation in amino benzenes polluted water is processed described in claim 1.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103667144B (en) * | 2013-12-11 | 2015-08-19 | 山东华亚环保科技有限公司 | One way of life sewage treatment microbial inoculant |
| CN105621626B (en) * | 2014-11-05 | 2018-09-28 | 江苏元捷环境科技有限公司 | A kind of high-concentration chemical industry sewage composite bacteria agent and its application |
| CN104529061B (en) * | 2014-12-13 | 2016-02-17 | 山东永泰化工有限公司 | Rubber accelerant wastewater treatment process |
| CN104694427B (en) * | 2015-02-12 | 2018-04-06 | 青岛楷博晶瑞生物科技有限公司 | A kind of preparation technology for being used to repair the microorganism formulation of nitrotoleune contaminated soil |
| CN105543150B (en) * | 2016-02-22 | 2020-03-13 | 杭州富阳高博信息技术服务有限公司 | Bioremediation preparation for aniline polluted water |
| CN108504585B (en) * | 2017-08-17 | 2021-05-14 | 北京沃太斯环保科技发展有限公司 | Benzene degrading bacterium for treating atmospheric pollution and preparation method and application thereof |
| CN112553112B (en) * | 2020-12-19 | 2023-01-17 | 武汉水之国环保科技有限公司 | Denitrifying bacillus subtilis and application thereof in strain detoxification |
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