CN101899397A - Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application - Google Patents
Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application Download PDFInfo
- Publication number
- CN101899397A CN101899397A CN 201010218533 CN201010218533A CN101899397A CN 101899397 A CN101899397 A CN 101899397A CN 201010218533 CN201010218533 CN 201010218533 CN 201010218533 A CN201010218533 A CN 201010218533A CN 101899397 A CN101899397 A CN 101899397A
- Authority
- CN
- China
- Prior art keywords
- culture
- composite
- bacillus
- highly effective
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a composite efficient microbial preparation for treating difficultly-degradable waste water. The composite efficient microbial preparation comprises the following active ingredients of: bacillus, pseudomonas, alcaligenes eutrophus, aspergillus and yeast. The composite efficient microbial preparation has a unique treating effect on high-concentration organic sewage and high ammonia and nitrogen sewage which are difficult to treat by the conventional activated sludge process and contain macromolecules and difficultly-degradable harmful constituents.
Description
Technical field
The present invention relates to a kind of composite and highly effective microorganism preparation and preparation and application that used water difficult to degradate is handled that be used for.
Background technology
The biologic treating technique of waste water is the dominant technology of current field of waste water treatment, and it is improving the water body environment quality, preventing from bringing into play important effect aspect the water pollution as a kind of economy, reasonable, effective wastewater processing technology.
The biological treatment of waste water is the metabolism that utilizes microorganism, and the decomposition and inversion pollutants in waste water reaches the purpose that purifies water.Its core is to cultivate the superior microorganism flora that domestication is fit to waste water characteristic, and maintenance and contaminated wastewater the substrate concentration microbial biomass and the activity that adapt, the superior microorganism flora is handled in best degrading activity, give full play to its degradation function, improve the biological treatment ability and the processing efficiency of system to greatest extent.
Nature culturing sludge acclimated microorganism is generally adopted in the biological treatment of waste water at present, and active sludge has been brought into play important role as traditional microorganism culturing domestication mode in field of waste water treatment especially municipal wastewater process field.But, in recent decades because the continuous development of industry, new technology, the continuous use of novel material, make the pollutent in the environment become complicated more, particularly a large amount of artificial-synthetic compounds enter environment, this class material mainly is an alkanes, olefines, alicyclic hydrocarbon type, arene, the organism of synthetic, especially macromole, difficult degradation, hazardous and noxious substances such as phenols (phenol, chlorophenol, cresols, nitrophenol etc.), arene (benzene, toluene, dimethylbenzene, oil of mirbane, phenol type substances, naphthalene, anthracene, quinone etc.), the cyanogen class, amine and ammonia nitrogen etc. are along with the waste water or the solid useless environment that enters of factory's discharging, because complicacy of these these body structures of material and biological strange property, can not be utilized by microbiological degradation at short notice, traditional method of wastewater treatment is cultivated acclimated microorganism with active sludge and can not be removed these pollutents effectively, these materials accumulate in environment for a long time, the ecotope of depending on for existence to us causes very big pollution, brings very big harm for human physical and mental health.In addition, because the continuous development of urban construction, urban land is nervous year by year, and the traditional activated sludge process processing technological flow is long, and floor space is big, and contradictions such as complicated operation are also outstanding day by day.And can bring into play the potentiality of microorganism well at the special composite and highly effective microorganism preparation of preparing of the pollutent of these difficult degradations, can effectively degrade and remove these difficult degradations, poisonous and hazardous pollutent, purify our ecotope, effectively address these problems and contradiction.
Composite and highly effective microorganism preparation is exactly efficient bacterial strain that filters out from nature at different difficult degradation pollutents or the engineering bacteria of cultivating domestication by the biotechnology means, according to special formula, with the various bacterial classifications that can form dominant microflora, be mixed with high-efficiency microorganism preparation, be added in the Waste Water Treatment by a certain amount of, can cultivate at short notice and tame out the dominant microflora that is fit to waste water characteristic, improve microbic activity and quantity greatly, quicken the degraded of microorganism to pollutent, to improve the biological treatment efficient of system, guarantee system stable operation.So the high-effective microorganism Study on Technology is rapid with the application development in recent years, has broad application prospects.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of active high, degradation capability is strong, processing efficiency is high, to composite and highly effective microorganism preparation and the preparation and the application that used water difficult to degradate is handled that be used for of difficult degradation, hazardous and noxious substances and ammonia nitrogen degradation ability and strong shock resistance.Above-mentioned used water difficult to degradate is meant the organism that contains alkanes, olefines, alicyclic hydrocarbon type, arene, synthetic, especially the waste water of macromole, difficult degradation, hazardous and noxious substances such as phenols (phenol, chlorophenol, cresols, nitrophenol etc.), arene (benzene,toluene,xylene, oil of mirbane, phenol type substances, naphthalene, anthracene, quinone etc.), cyanogen class, amine and ammonia nitrogen.
The activeconstituents of composite and highly effective microorganism preparation provided by the invention comprises: genus bacillus, pseudomonas, Alcaligenes, aspergillus and yeast.
As preferably, above-mentioned genus bacillus is a kind of bacterial classification of bacillus or the arbitrary combination of two or more bacterial classifications, described pseudomonas is a kind of bacterial classification of Rhodopseudomonas or the arbitrary combination of two or more bacterial classifications, above-mentioned Alcaligenes is a kind of or its combination in Bacillus foecalis alkaligenes and the denitration Alcaligenes, above-mentioned aspergillus is a kind of or its combination in aspergillus niger and the aspergillus oryzae, and above-mentioned yeast is a kind of or its combination in yeast saccharomyces cerevisiae and the Candida utilis.
As preferably, above-mentioned genus bacillus is subtilis, bacillus amyloliquefaciens, separate one or more arbitrary combination in sugared clostridium and the Bacillus sphaericus; Above-mentioned pseudomonas is a kind of in Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas alcaligenes and the denitrifying pseudomonas or combination that they are two or more.
As preferably, the mentioned microorganism composite fungus agent is made up of the culture of bacterium and fungi, comprises following compositions in portion by weight:
Bacillus culture 25～35,
Rhodopseudomonas culture 15～25,
Alkaligenes culture 10～20,
Aspergillus culture 10～20,
Saccharomycodes culture 10～20,
Wherein, described bacillus culture is the culture of a kind of bacterial classification or two or more bacterial classification combination in the bacillus, and wherein the content of genus bacillus is hundred million of 50-100/gram; Described Rhodopseudomonas culture is the culture of a kind of bacterial classification or two or more bacterial classification combination in the Rhodopseudomonas, and wherein the content of pseudomonas is hundred million of 50-100/gram; Described Alkaligenes culture is the culture of a kind of or its combination in Bacillus foecalis alkaligenes and the denitration Alcaligenes, and wherein the content of Alcaligenes is hundred million of 50-100/gram; Described Aspergillus culture is the culture of a kind of or its combination in aspergillus niger and the aspergillus oryzae, wherein hundred million/gram of aspergillar content 10-50; Described Saccharomycodes culture is the culture of a kind of or its combination in yeast saccharomyces cerevisiae and the Candida utilis, and wherein saccharomycetic content is hundred million of 10-50/gram.
The invention provides a kind of method for preparing above-mentioned composite and highly effective microorganism preparation, comprise the steps:
1) from bacillus, Rhodopseudomonas, Alkaligenes, Aspergillus and Saccharomycodes, respectively selects one or more bacterial classifications, carry out seed enlarged culturing step by step respectively, obtain the kind daughter bacteria liquid of enlarged culturing;
2) the kind daughter bacteria liquid of the enlarged culturing that step 1) is obtained inserts fermentor tank respectively, inoculum size by weight or volumeter, be the 5-10% of fermention medium, different Pseudomonas carries out fermentation culture respectively separately, and the bacterial classification more than 2 or 2 of same Pseudomonas carries out mixing fermentation culture;
3) fermention medium initial p H is adjusted to 6.0-8.0, and controlled temperature 30-40 ℃, fermentation time 24-48 hour, fermenting process adopted mechanical stirring or feeds the filtrated air oxygen supply;
4) with bacillus culture, Rhodopseudomonas culture, Alkaligenes culture, Aspergillus culture and the Saccharomycodes culture of described amount, mix, obtain composite and highly effective microorganism preparation, preserve.
As preferably, step 2) described fermention medium is a liquid nutrient medium, contains the aqueous solution of following concentration composition: glucose 40000-60000mg/L, saltpetre 1500-2500mg/L, ammonium nitrate 1500-2000
Mg/L, calcium chloride 400-500mg/L, sal epsom 450-500mg/L, potassium primary phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganous sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, potassiumiodide 0.5-1.0mg/L, Sodium orthomolybdate 0.2-0.3mg/L and copper sulfate 0.02-0.03mg/L.The described composite and highly effective microorganism preparation of step 4) is the liquid composite and highly effective microorganism preparation, preserves with canning sealing.
As preferably, step 2) described fermention medium is made up of 1: 1 solid medium of weight ratio and liquid nutrient medium, wherein, described solid medium is made of by weight percentage following ingredients: wheat bran 80-90%, Semen Maydis powder 5-10% and soybean cake powder 5-10%, described liquid nutrient medium are the aqueous solution that contains following concentration composition: glucose 40000-60000mg/L, saltpetre 1500-2500mg/L, ammonium nitrate 1500-2000mg/L, calcium chloride 400-500mg/L, sal epsom 450-500mg/L, potassium primary phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganous sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, potassiumiodide 0.5-1.0mg/L, Sodium orthomolybdate 0.2-0.3mg/L and copper sulfate 0.02-0.03
mg/L。The described composite and highly effective microorganism preparation of step 4) is the solid composite and highly effective microorganism preparation, the oven dry packed preservation in back under 40-45 ℃ of temperature.
The present invention also provides the application of above-mentioned composite and highly effective microorganism preparation in used water difficult to degradate is handled, and above-mentioned composite and highly effective microorganism preparation is added in the wastewater treatment biochemistry pool.Addition method and dosage are: activated sludge process adds by the biochemistry pool volume, and every cubic metre of biochemistry pool volume adds above-mentioned composite and highly effective microorganism preparation 20-30 gram at every turn, adds every day 1 time, adds continuously 10-15 days; Biomembrance process adds by the volume of filler, and every cubic metre of packing volume adds above-mentioned composite and highly effective microorganism preparation 30-50 gram at every turn, adds every day 1 time, adds continuously 10-15 days.
The present invention can reach following technique effect:
Composite and highly effective microorganism preparation of the present invention contains the multiple microorganism that the difficult degradation pollutent is had good degradation capability, active high, biomass is big, breeding is fast, add in Waste Water Treatment, the cultivation acclimation period is short, can in 10-30 days, fast culture tame out the superior microorganism flora that is fit to waste water characteristic, especially to macromole, difficult degradation, hazardous and noxious substances such as phenols (phenol, chlorophenol, cresols, nitrophenol etc.), arene (benzene, toluene, dimethylbenzene, oil of mirbane, phenol type substances, naphthalene, anthracene, quinone etc.), the cyanogen class, amine and ammonia nitrogen, phosphorus etc. have good degradation effect, and reluctant high concentration organic sewage of traditional activated sludge process and high-ammonia-nitrogen sewage are had unique treatment effect.Be particularly suitable for the cultivation domestication of newly-built industrial sewage treatment plant superior microorganism and the dilatation and the technological transformation of old sewage work, can improve and handle the water yield and water quality treatment, reduce working cost, the assurance qualified discharge.Be simple, economic, the effective modern technique that quick startup of new system and old sewage work carry out extending capacity reformation, can save investment cost greatly, obtain the optimum handling effect.
Embodiment
The invention will be further described below in conjunction with specific embodiment, can be implemented so that those skilled in the art can better understand the present invention also, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1:
1, selects subtilis, Pseudomonas fluorescens, Bacillus foecalis alkaligenes, aspergillus niger and yeast saccharomyces cerevisiae, above-mentioned bacterial classification is carried out seed enlarged culturing step by step respectively.
2, add the clean tap water of 5000mL in the container after disinfects, add glucose 200g, saltpetre 7.5g, ammonium nitrate 7.5g, calcium chloride 2.0g, sal epsom 2.25g, potassium primary phosphate 750mg, inositol 750mg, ferrous sulfate 125mg, manganous sulfate 100mg, zinc sulfate 25mg, boric acid 25mg, potassiumiodide 2.5mg, Sodium orthomolybdate 1.0mg, copper sulfate 0.1mg respectively, fully stirring and dissolving, mix, regulate PH to 6.0.Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 1000mL.
3, in each beaker, inoculate the kind liquid 100mL of subtilis, Pseudomonas fluorescens, Bacillus foecalis alkaligenes, aspergillus niger and yeast saccharomyces cerevisiae enlarged culturing successively, put into 30 ℃ thermostat container fermentation culture 24 hours after stirring, stirred 1 time every 2 hours between yeast phase, control PH is to 6.0-8.0 in the fermenting process, after fermentation was finished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram.
4, by weight with cultured fermented product, press following mixed: 25 parts of Bacillus subtillis cultures, 25 parts of Pseudomonas fluorescens cultures, 20 parts of Bacillus foecalis alkaligenes cultures, 10 parts of aspergillus niger cultures, 10 parts of yeast saccharomyces cerevisiae cultures mix the back canning sealing.
Embodiment 2:
1, choose shuttles shape genus bacillus, Pseudomonas alcaligenes, denitration Alcaligenes, aspergillus oryzae and Candida utilis bacterium are with above-mentioned bacterial classification, carry out seed enlarged culturing step by step respectively.
2, the clean tap water that adds 2500mL in the container after disinfects, add glucose 150g, saltpetre 6.25g, ammonium nitrate 5g, calcium chloride 1.25g, sal epsom 1.25g, potassium primary phosphate 500mg, inositol 625mg, ferrous sulfate 87.5mg, manganous sulfate 62 respectively, 5mg, zinc sulfate 25mg, boric acid 25mg, potassiumiodide 2.5mg, Sodium orthomolybdate 0.75mg, copper sulfate 0.075mg, fully stirring and dissolving, mix, regulate PH to 8.0.Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 500mL.
3, in the beaker of above-mentioned 5 nutrient solutions, add wheat bran 400g, Semen Maydis powder 50g, soybean cake powder 50g after sterilising treatment respectively, mixing and stirring.
4, in each beaker, inoculate the kind liquid 50mL of Bacillus sphaericus, Pseudomonas alcaligenes, denitration Alcaligenes, aspergillus oryzae and Candida utilis bacterium enlarged culturing successively, put into 40 ℃ thermostat container fermentation culture 48 hours after stirring, stirred 1 time every 2 hours between yeast phase, control PH is to 6.0-8.0 in the fermenting process, after fermentation was finished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram.
5, by weight with cultured fermented product, press following mixed: 35 parts of Bacillus sphaericus cultures, 15 parts of Pseudomonas alcaligenes cultures, 10 parts of denitration Alcaligenes cultures, 20 parts of aspergillus oryzae cultures, 20 parts of Candida utilis bacterium cultures.It is packed to mix oven dry back under 40-45 ℃ of temperature, back.
Embodiment 3:
1, selects the bacillus amyloliquefaciens of bacillus to conciliate Bacillus foecalis alkaligenes and denitration Alcaligenes, the aspergillus niger of Aspergillus and the yeast saccharomyces cerevisiae and the Candida utilis of aspergillus oryzae and Saccharomycodes of the Pseudomonas aeruginosa of sugared clostridium, Rhodopseudomonas and denitrifying pseudomonas, Alkaligenes, above-mentioned bacterial classification, each bacterial classification are carried out seed enlarged culturing step by step respectively.
2, add the clean tap water of 2500mL in the container after disinfects, add glucose 125g, saltpetre 5g, ammonium nitrate 4.5g, calcium chloride 1.2g, sal epsom 1.2g, potassium primary phosphate 450mg, inositol 500mg, ferrous sulfate 75mg, manganous sulfate 55mg, zinc sulfate 20mg, boric acid 20mg, potassiumiodide 2mg, Sodium orthomolybdate 0.7mg, copper sulfate 0.07mg respectively, fully stirring and dissolving, mix, regulate PH to 7.0.Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 500mL.
3, in the beaker of above-mentioned 5 nutrient solutions, add wheat bran 450g, Semen Maydis powder 25g, soybean cake powder 25g after sterilising treatment respectively, mixing and stirring.
4, in each beaker, inoculate the kind liquid of bacillus, Rhodopseudomonas, Alkaligenes, Aspergillus, Saccharomycodes enlarged culturing successively, the kind liquid of two strain expanded culture in each Pseudomonas meets 50mL respectively, put into 35 ℃ thermostat container fermentation culture 36 hours after stirring, stirred 1 time every 2 hours between yeast phase, control PH 6.0-8.0 in the fermenting process, after fermentation was finished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram.
5, by weight with cultured fermented product, press following mixed: 30 parts of Bacillus cultures, 20 parts of Rhodopseudomonas cultures, 15 parts of Alkaligenes cultures, 17 parts of Aspergillus cultures, 18 parts of Saccharomycodes cultures, packed after the oven dry under 40-45 ℃ of temperature after mixing.
The present invention enumerates the several application example to further specify the present invention according to example:
Application examples 1: adopt composite and highly effective microorganism preparation of the present invention to handle high saliferous aniline, oil of mirbane, nitrophenol wastewater, treatment process is anaerobic biofilter+BAF, add composite and highly effective microorganism preparation 30 grams that embodiment 1 makes by every cubic metre of filtrate at every turn, add every day 1 time, added continuously 15 days; Cultivate domestication after 20 days, treatment effect such as following table:
| Oil of mirbane | 1478 | 698.4 | 1.0 | 0.2 | 60-90 | 99.97 |
| Nitrophenol | 3000 | 1700 | 2.6 | 0.23 | 60-90 | 99.98 |
| Chlorion | 38000 |
Application examples 2: adopt composite and highly effective microorganism preparation of the present invention to handle coal chemical industrial waste water, treatment process is the filter of A/O+ precipitation+aeration and biological, the A/O pond adds composite and highly effective microorganism preparation 20 grams that embodiment 2 makes by every cubic metre of biochemistry pool volume at every turn, add every day 1 time, added continuously 15 days; BAF adds this composite and highly effective microorganism preparation 40 grams by every cubic meter filler, adds every day 1 time, adds continuously 10 days; Cultivate domestication after 25 days, treatment effect such as following table:
Application examples 3: adopt composite and highly effective microorganism preparation Treatment of Wastewater in Coking of the present invention, treatment process is acidification hydrolization+BAF+MBR, add composite and highly effective microorganism preparation 50 grams that embodiment 3 makes by every cubic metre of filler at every turn, add every day once, added continuously 15 days; Cultivate domestication after 30 days, treatment effect such as following table:
Application examples 4: adopt composite and highly effective microorganism preparation of the present invention to handle medicine intermediate waste water, treatment process is UASB+ catalytic oxidation+BAF, UASB adds composite and highly effective microorganism preparation 30 grams that embodiment 3 makes by every cubic metre of biochemistry pool volume at every turn, add every day 1 time, added continuously 15 days; Catalytic oxidation and BAF add this composite and highly effective microorganism preparation 30 grams by every cubic metre of filler, add every day 1 time, add continuously 15 days; Cultivate domestication after 30 days, treatment effect such as following table:
The above embodiment is the preferred embodiment that proves absolutely that the present invention lifts, and protection scope of the present invention is not limited thereto.Being equal to that those skilled in the art are done on basis of the present invention substitutes or conversion, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.
Claims (10)
1. one kind is used for the composite and highly effective microorganism preparation that used water difficult to degradate is handled, and it is characterized in that the activeconstituents of described composite and highly effective microorganism preparation comprises: genus bacillus, pseudomonas, Alcaligenes, aspergillus and yeast.
2. composite and highly effective microorganism preparation according to claim 1, it is characterized in that, described genus bacillus is a kind of bacterial classification or the two or more bacterial classification arbitrary combination of bacillus, described pseudomonas is a kind of bacterial classification or the two or more bacterial classification arbitrary combination of Rhodopseudomonas, described Alcaligenes is a kind of or its arbitrary combination in Bacillus foecalis alkaligenes and the denitration Alcaligenes, described aspergillus is a kind of or its arbitrary combination in aspergillus niger and the aspergillus oryzae, and described yeast is a kind of or its arbitrary combination in yeast saccharomyces cerevisiae and the Candida utilis.
3. composite and highly effective microorganism preparation according to claim 1 is characterized in that, described genus bacillus is subtilis, bacillus amyloliquefaciens, separate one or more arbitrary combination in sugared clostridium and the Bacillus sphaericus; Described pseudomonas is a kind of in Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas alcaligenes and the denitrifying pseudomonas or combination that they are two or more.
4. composite and highly effective microorganism preparation according to claim 1 is characterized in that described complex microbial inoculum is made up of the culture of bacterium and fungi, comprises following compositions in portion by weight:
Bacillus culture 25～35,
Rhodopseudomonas culture 15～25,
Alkaligenes culture 10～20,
Aspergillus culture 10～20,
Saccharomycodes culture 10～20,
Wherein, described bacillus culture is the culture of a kind of bacterial classification or two or more bacterial classification combination in the bacillus, and wherein the content of genus bacillus is hundred million of 50-100/gram; Described Rhodopseudomonas culture is the culture of a kind of bacterial classification or two or more bacterial classification combination in the Rhodopseudomonas, and wherein the content of pseudomonas is hundred million of 50-100/gram; Described Alkaligenes culture is the culture of a kind of or its combination in Bacillus foecalis alkaligenes and the denitration Alcaligenes, and wherein the content of Alcaligenes is hundred million of 50-100/gram; Described Aspergillus culture is the culture of a kind of or its combination in aspergillus niger and the aspergillus oryzae, wherein hundred million/gram of aspergillar content 10-50; Described Saccharomycodes culture is the culture of a kind of or its combination in yeast saccharomyces cerevisiae and the Candida utilis, and wherein saccharomycetic content is hundred million of 10-50/gram.
5. prepare the method for the described composite and highly effective microorganism preparation of claim 1～4, it is characterized in that, comprise the steps:
1) from bacillus, Rhodopseudomonas, Alkaligenes, Aspergillus and Saccharomycodes, respectively selects one or more bacterial classifications, carry out seed enlarged culturing step by step respectively, obtain the kind daughter bacteria liquid of enlarged culturing;
2) the kind daughter bacteria liquid of the enlarged culturing that step 1) is obtained inserts fermentor tank respectively, inoculum size by weight or volumeter, be the 5-10% of fermention medium, different Pseudomonas carries out fermentation culture respectively separately, and the bacterial classification more than 2 or 2 of same Pseudomonas carries out mixing fermentation culture;
3) fermention medium PH is adjusted to 6.0-8.0, and controlled temperature 30-40 ℃, fermentation time 24-48 hour, the air oxygen supply after fermenting process adopts mechanical stirring or feeds filtration;
4) with bacillus culture, Rhodopseudomonas culture, Alkaligenes culture, Aspergillus culture and the Saccharomycodes culture of described amount, mix, obtain composite and highly effective microorganism preparation, preserve.
6. method according to claim 5, it is characterized in that, step 2) described fermention medium is a liquid nutrient medium, contains the aqueous solution of following concentration composition: glucose 40000-60000mg/L, saltpetre 1500-2500mg/L, ammonium nitrate 1500-2000mg/L, calcium chloride 400-500mg/L, sal epsom 450-500mg/L, potassium primary phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganous sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, potassiumiodide 0.5-1.0mg/L, Sodium orthomolybdate 0.2-0.3mg/L and copper sulfate 0.02-0.03mg/L.
7. method according to claim 6 is characterized in that, the described composite and highly effective microorganism preparation of step 4) is the liquid composite and highly effective microorganism preparation, preserves with canning sealing.
8. method according to claim 5, it is characterized in that, step 2) described fermention medium is made up of 1: 1 solid medium of weight ratio and liquid nutrient medium, wherein, described solid medium is made of by weight percentage following ingredients: wheat bran 80-90%, Semen Maydis powder 5-10% and soybean cake powder 5-10%, described liquid nutrient medium are the aqueous solution that contains following concentration composition: glucose 40000-60000mg/L, saltpetre 1500-2500mg/L, ammonium nitrate 1500-2000mg/L, calcium chloride 400-500mg/L, sal epsom 450-500mg/L, potassium primary phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganous sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, potassiumiodide 0.5-1.0mg/L, Sodium orthomolybdate 0.2-0.3mg/L and copper sulfate 0.02-0.03mg/L.
9. method according to claim 8 is characterized in that, the described composite and highly effective microorganism preparation of step 4) is the solid composite and highly effective microorganism preparation, the oven dry packed preservation in back under 40-45 ℃ of temperature.
10. the application of the described composite and highly effective microorganism preparation of claim 1～3 in used water difficult to degradate is handled is characterized in that, described composite and highly effective microorganism preparation is added in the wastewater treatment biochemistry pool.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010218533 CN101899397B (en) | 2010-07-06 | 2010-07-06 | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010218533 CN101899397B (en) | 2010-07-06 | 2010-07-06 | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101899397A true CN101899397A (en) | 2010-12-01 |
| CN101899397B CN101899397B (en) | 2012-12-05 |
Family
ID=43225319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010218533 Active CN101899397B (en) | 2010-07-06 | 2010-07-06 | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101899397B (en) |
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766588A (en) * | 2012-04-13 | 2012-11-07 | 轻工业环境保护研究所 | Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof |
| CN102864107A (en) * | 2012-09-26 | 2013-01-09 | 朱文霞 | Microbial preparation for rehabilitating phenyl amine polluted water body |
| CN102864109A (en) * | 2012-09-26 | 2013-01-09 | 李丽萍 | Preparation for trinitrotoluene-contained sewage treatment and using method of preparation |
| CN102888374A (en) * | 2012-09-26 | 2013-01-23 | 闫家怡 | Biological agent for processing industrial sewage and preparation method and application |
| CN103214143A (en) * | 2013-03-21 | 2013-07-24 | 杭州天凯生物科技有限公司 | High efficiency combined restoration technology for urban river water bodies |
| CN103351061A (en) * | 2013-05-15 | 2013-10-16 | 钱臻 | Organic pollution water body bioremediation agent and preparation method thereof |
| CN103667144A (en) * | 2013-12-11 | 2014-03-26 | 山东华亚环保科技有限公司 | Domestic sewage treatment microbial inoculant |
| CN105002127A (en) * | 2015-08-24 | 2015-10-28 | 周俊冉 | Composite bacterium and application thereof to treatment of garlic processing wastewater |
| CN105039228A (en) * | 2015-09-05 | 2015-11-11 | 内蒙古阜丰生物科技有限公司 | Biological agent for glutamate wastewater treatment |
| CN105036352A (en) * | 2015-07-07 | 2015-11-11 | 东南大学 | Microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution |
| CN105084557A (en) * | 2015-09-05 | 2015-11-25 | 内蒙古阜丰生物科技有限公司 | Technology for removing industrial COD and ammonia nitrogen from monosodium glutamate wastewater |
| CN105174654A (en) * | 2015-10-16 | 2015-12-23 | 中国科学院水生生物研究所 | Deodorization method for landscape water body |
| CN105399213A (en) * | 2015-12-12 | 2016-03-16 | 常州大学 | Microbial degradation method for phenol-containing waste water |
| CN107164276A (en) * | 2017-06-21 | 2017-09-15 | 北京大学 | One plant tolerance zinc ion toxicity pseudomonas aeruginosa and its application |
| CN107265658A (en) * | 2017-06-22 | 2017-10-20 | 浙江工商大学 | Regulate and control pseudomonas aeruginosa aerobic denitrification using quorum sensing in the method except nitrate in waste water |
| CN107653209A (en) * | 2017-11-15 | 2018-02-02 | 中国环境科学研究院 | Phenol degrading microbial bacterial agent, immobilized spherule and preparation method |
| CN108441444A (en) * | 2018-03-22 | 2018-08-24 | 中电建水环境治理技术有限公司 | A kind of complex micro organism fungicide administered suitable for black and odorous water |
| CN108624531A (en) * | 2018-05-15 | 2018-10-09 | 北京师范大学 | A kind of complex microorganism water purification agent and its application |
| CN109852557A (en) * | 2018-11-30 | 2019-06-07 | 北京国环清华环境工程设计研究院有限公司 | A kind of composite bacteria agent and the preparation method and application thereof for the processing of cities and towns storage waste stabilisation |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
| CN111137972A (en) * | 2020-02-26 | 2020-05-12 | 北京联合大学 | Method for quickly generating activated sludge for wastewater treatment |
| CN113151010A (en) * | 2021-05-07 | 2021-07-23 | 马东兵 | Microbial compound bacterium preparation and preparation method thereof |
| CN114230019A (en) * | 2021-10-25 | 2022-03-25 | 盐城工学院 | Application of probiotic activated sludge in sewage treatment |
| CN114590911A (en) * | 2022-05-09 | 2022-06-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Denitrifying microbial inoculum with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4159944A (en) * | 1978-02-13 | 1979-07-03 | Erickson Lennart G | Wastewater energy recycling method |
-
2010
- 2010-07-06 CN CN 201010218533 patent/CN101899397B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4159944A (en) * | 1978-02-13 | 1979-07-03 | Erickson Lennart G | Wastewater energy recycling method |
Non-Patent Citations (2)
| Title |
|---|
| 《中国环境保护优秀论文集（2005）下册》 20051231 李清华等 复合微生物菌剂的研究应用现状 , 2 * |
| 《广州环境科学》 20060313 刘达伟等 复合菌种在污水处理中的应用 第21卷, 第1期 2 * |
Cited By (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766588B (en) * | 2012-04-13 | 2014-01-01 | 轻工业环境保护研究所 | Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof |
| CN102766588A (en) * | 2012-04-13 | 2012-11-07 | 轻工业环境保护研究所 | Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof |
| CN102864107A (en) * | 2012-09-26 | 2013-01-09 | 朱文霞 | Microbial preparation for rehabilitating phenyl amine polluted water body |
| CN102864109A (en) * | 2012-09-26 | 2013-01-09 | 李丽萍 | Preparation for trinitrotoluene-contained sewage treatment and using method of preparation |
| CN102888374A (en) * | 2012-09-26 | 2013-01-23 | 闫家怡 | Biological agent for processing industrial sewage and preparation method and application |
| CN102864109B (en) * | 2012-09-26 | 2014-02-05 | 李丽萍 | Preparation for trinitrotoluene-contained sewage treatment and using method of preparation |
| CN102864107B (en) * | 2012-09-26 | 2014-08-13 | 朱文霞 | Microbial preparation for rehabilitating phenyl amine polluted water body |
| CN103214143A (en) * | 2013-03-21 | 2013-07-24 | 杭州天凯生物科技有限公司 | High efficiency combined restoration technology for urban river water bodies |
| CN103351061B (en) * | 2013-05-15 | 2014-12-24 | 钱臻 | Organic pollution water body bioremediation agent and preparation method thereof |
| CN103351061A (en) * | 2013-05-15 | 2013-10-16 | 钱臻 | Organic pollution water body bioremediation agent and preparation method thereof |
| CN103667144A (en) * | 2013-12-11 | 2014-03-26 | 山东华亚环保科技有限公司 | Domestic sewage treatment microbial inoculant |
| CN103667144B (en) * | 2013-12-11 | 2015-08-19 | 山东华亚环保科技有限公司 | One way of life sewage treatment microbial inoculant |
| CN105036352A (en) * | 2015-07-07 | 2015-11-11 | 东南大学 | Microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution |
| CN105036352B (en) * | 2015-07-07 | 2017-03-15 | 东南大学 | A kind of microbial process for repairing aromatic hydrocarbons heavy metal ion combined pollution |
| CN105002127A (en) * | 2015-08-24 | 2015-10-28 | 周俊冉 | Composite bacterium and application thereof to treatment of garlic processing wastewater |
| CN105039228A (en) * | 2015-09-05 | 2015-11-11 | 内蒙古阜丰生物科技有限公司 | Biological agent for glutamate wastewater treatment |
| CN105084557A (en) * | 2015-09-05 | 2015-11-25 | 内蒙古阜丰生物科技有限公司 | Technology for removing industrial COD and ammonia nitrogen from monosodium glutamate wastewater |
| CN105174654A (en) * | 2015-10-16 | 2015-12-23 | 中国科学院水生生物研究所 | Deodorization method for landscape water body |
| CN105399213A (en) * | 2015-12-12 | 2016-03-16 | 常州大学 | Microbial degradation method for phenol-containing waste water |
| CN107164276B (en) * | 2017-06-21 | 2020-08-04 | 北京大学 | Zinc ion toxicity-resistant pseudomonas aeruginosa and application thereof |
| CN107164276A (en) * | 2017-06-21 | 2017-09-15 | 北京大学 | One plant tolerance zinc ion toxicity pseudomonas aeruginosa and its application |
| CN107265658A (en) * | 2017-06-22 | 2017-10-20 | 浙江工商大学 | Regulate and control pseudomonas aeruginosa aerobic denitrification using quorum sensing in the method except nitrate in waste water |
| CN107653209A (en) * | 2017-11-15 | 2018-02-02 | 中国环境科学研究院 | Phenol degrading microbial bacterial agent, immobilized spherule and preparation method |
| CN108441444A (en) * | 2018-03-22 | 2018-08-24 | 中电建水环境治理技术有限公司 | A kind of complex micro organism fungicide administered suitable for black and odorous water |
| CN108441444B (en) * | 2018-03-22 | 2020-09-25 | 中电建生态环境集团有限公司 | Compound microbial agent suitable for black and odorous water treatment |
| CN108624531A (en) * | 2018-05-15 | 2018-10-09 | 北京师范大学 | A kind of complex microorganism water purification agent and its application |
| CN109852557A (en) * | 2018-11-30 | 2019-06-07 | 北京国环清华环境工程设计研究院有限公司 | A kind of composite bacteria agent and the preparation method and application thereof for the processing of cities and towns storage waste stabilisation |
| CN110156174B (en) * | 2019-05-30 | 2022-01-25 | 杭州秀川科技有限公司 | Mixed strain fermentation biological pretreatment method for high-concentration high-salt pharmaceutical wastewater |
| CN110156174A (en) * | 2019-05-30 | 2019-08-23 | 杭州秀川科技有限公司 | A kind of fermented by mixed bacterium biologic pretreatment method for highly concentrated pharmacy waste water with high salt |
| CN111137972A (en) * | 2020-02-26 | 2020-05-12 | 北京联合大学 | Method for quickly generating activated sludge for wastewater treatment |
| CN111137972B (en) * | 2020-02-26 | 2022-04-19 | 北京联合大学 | Method for quickly generating activated sludge for wastewater treatment |
| CN113151010A (en) * | 2021-05-07 | 2021-07-23 | 马东兵 | Microbial compound bacterium preparation and preparation method thereof |
| CN113151010B (en) * | 2021-05-07 | 2023-03-14 | 马东兵 | Microbial compound bacterium preparation and preparation method thereof |
| CN114230019A (en) * | 2021-10-25 | 2022-03-25 | 盐城工学院 | Application of probiotic activated sludge in sewage treatment |
| CN114590911A (en) * | 2022-05-09 | 2022-06-07 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Denitrifying microbial inoculum with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101899397B (en) | 2012-12-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101899397B (en) | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application | |
| CN104528955B (en) | A kind of microorganism formulation for repairing chemical fertilizer factory's sewage | |
| CN102888374B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN102888345B (en) | Biological agent for processing industrial sewage and preparation method and application | |
| CN102616941B (en) | Sea cucumber microecological water quality conditioning agent and method for preparing same | |
| CN104478098B (en) | For the treatment of the microbial preparation of coal chemical industrial waste water | |
| CN102071141B (en) | Denitrified microbial agent as well as preparation method and application thereof | |
| CN106635861B (en) | A kind of de- COD denitrification microorganism microbial inoculum of salt tolerant and preparation method thereof | |
| CN107873017B (en) | Microbial compositions and methods for denitrification at high dissolved oxygen levels | |
| CN101050423A (en) | Composite preparation microbiological, and preparation method | |
| CN105174491B (en) | For deodorant microorganism formulation of water body and its preparation method and application | |
| CN106745828A (en) | A kind of broad-spectrum biological preparation administered for black and odorous water and its application method | |
| CN105331554A (en) | Compound microorganism bacterium agent and preparation method and application thereof | |
| CN102776138A (en) | Compound microorganism preparation for materialized sludge treatment and method for preparing same | |
| CN104694443A (en) | Improved biological microbial agent for disposing industrial sewage and preparation method and application thereof | |
| CN104086001A (en) | Treatment process applicable to industrial wastewater and application of industrial wastewater in preparation of bacterial manure | |
| CN105505831A (en) | Compound microbial inoculum for sewage purification and preparation method thereof | |
| CN106635883A (en) | Composite microbial preparation for treating watercourse organic matter | |
| CN104528956A (en) | Composite microbial inoculant for treating coal chemical industry sewage and application thereof | |
| KR101191326B1 (en) | Method for treating organic waste using carrier | |
| CN104496031B (en) | A kind of sewage disposal is held concurrently water plant planting matrix fill preparation method and application | |
| CN104071902B (en) | Method for treating household wastewater | |
| CN102220240A (en) | PM-I sludge reduction microbial agent | |
| CN103305443B (en) | Preparation and method for restoring ammonia nitrogen-containing industrial sewage | |
| CN105152478B (en) | Treatment method of industrial wastewater produced during preparation of sodium glutamate through concentration isoelectric process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |