CN113151010A - Microbial compound bacterium preparation and preparation method thereof - Google Patents
Microbial compound bacterium preparation and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a microbial compound bacterium preparation and a preparation method thereof. The active ingredients of the microbial compound bacteria preparation comprise microbial floras of the following genera: bacillus, Pseudomonas, Lactobacillus, Torulopsis, Aspergillus, Rhizopus, Penicillium, Mucor, Trichoderma, Glutinosa, Azotobacter and Nitrosomonas. The microbial compound bacteria preparation contains various active bacteria, and by reasonable compatibility of various fungi and bacteria, the microbial compound bacteria preparation exists as a dominant bacteria group in a biological treatment section of sewage treatment, so that the organic pollutants COD and NH in sewage are obviously reduced3The content of pollutant components such as-N, TN, TP and the like realizes the waste treatment of municipal sewage and domestic garbage leachateThe high-efficiency purification of water, kitchen and kitchen waste wastewater, river black and odorous water and industrial wastewater.
Description
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to a microorganism composite bacteria preparation and a preparation method thereof.
Background
With the continuous promotion of the urbanization process and the increasing development of industrial economy, domestic sewage, garbage leachate wastewater, kitchen and kitchen garbage wastewater, industrial wastewater, river black and odorous water bodies and the like in the urban area are more and more, the sewage components are more and more complex and the pollution is more and more serious, and a large amount of domestic and industrial sewage continuously flows into rivers and permeates into underground water. The urban sewage not only contains organic pollutants such as proteins, saccharides and waste oil, but also contains a large amount of organic matters and inorganic matters, nitrogen and phosphorus pollution and the like, and the pollution has great negative effects on the health of a human body and the sustainable development of the society. Therefore, effective treatment and utilization of sewage is a common concern.
At present, people strive to adopt various physical, chemical or biological methods to treat sewage and reduce the content of various pollutants in urban water, so as to improve the water quality of the water and further relieve the severe current situation of fresh water resource supply. For example, CN105214359B discloses a sewage treatment system, wherein a filtering membrane bracket and a filtering membrane self-cleaning device are disposed in a sewage treatment tank, and the filtering membrane self-cleaning device can prevent the filtering membrane from hardening, thereby reducing the cleaning frequency and maintenance cost of the sewage treatment tank, and further improving the sewage treatment efficiency; CN103043873B discloses a membrane bioreactor/microbial fuel cell sewage treatment device, which adds a microbial fuel cell structure on the membrane bioreactor to greatly reduce the total nitrogen content after sewage treatment; CN101381151B discloses an embedding immobilized microorganism particle fluidized bed sewage treatment method, which realizes the conversion and degradation of organic matters in sewage under the action of enzyme generated by microorganisms by filling spherical filler particles embedded with microorganism flora in a sewage treatment device; CN208700644U discloses a microbial filtration membrane for sewage treatment, wherein a microbial culture layer is tightly attached to a microbial layer, so that microbes on the microbial membrane can absorb and decompose harmful components in sewage, thereby achieving a good filtration effect; CN112079474A discloses a sewage treatment method for sludge reduction, which adopts various ways such as filtration, flocculation and COD remover to reduce the sludge content and the factors generating sludge, thereby improving the sewage treatment effect; CN105884137B A sewage treatment method of immobilized microorganisms, which prepares immobilized microorganisms by modifying sodium alginate with graphene oxide and applies the immobilized microorganisms to sewage treatment equipment, improves the stability of the immobilized microorganisms and improves the adaptability of the sewage treatment equipment in different types of sewage; CN103570132B an ecological restoration method of eutrophic water, which comprises culturing aquatic plants in anaerobic microorganism group to form a layer of microbial film on the aquatic plants, and then placing the cultured aquatic plants in pretreated river water to purify water. Compared with the traditional physical and chemical treatment modes, the biological treatment mode, especially the treatment of various harmful substances in the sewage through the metabolism of microorganisms, is an environment-friendly treatment mode which is not easy to cause secondary pollution.
The conventional microorganism treatment method is to consume or decompose pollutants in the sewage by using the activity of microorganisms so as to achieve the purpose of decomposing or reducing the purified water body. However, since the current urban polluted water has complex conditions and diversified pollutants, effective purification effect is difficult to achieve by treating sewage with a single microbial strain.
Therefore, in view of the current situation of sewage with complex components, how to utilize the activity of microorganisms is an urgent problem to be solved.
Disclosure of Invention
The invention aims to provide a microbial compound bacteria preparation for sewage treatment, which is derived from various soil bacteria in soil and various activated sludge bacteria of activated sludge, and can effectively remove excessive nitrogen and phosphorus, organic matters, inorganic matters, heavy metals and other pollutants in sewage by reasonably matching various bacteria, thereby realizing the purification of sewage.
The purpose of the invention is realized by the following technical scheme:
according to one aspect of the present invention, there is provided a complex microbial preparation, the active ingredients of which include the microbial flora of the following genera: bacillus (Bacillus) bacteria, Pseudomonas (Pseudomonas) bacteria, Lactobacillus (Lactobacillus) bacteria, Torulopsis (Torulopsis) fungi, Aspergillus (Aspergillus) fungi, Rhizopus (Rhizopus) fungi, Penicillium (Penicillium) fungi, Mucor (Mucor) fungi, Trichoderma (Trichoderma) fungi, Glucomyces (Thiocapsa) bacteria, Azotobacter (Azotobacter) bacteria, and Nitrosomonas (Nitrosomonas) bacteria.
Wherein, the microbial compound bacteria preparation comprises 65 to 85 portions of bacillus bacteria, 2 to 10 portions of pseudomonas bacteria, 0.1 to 2.5 portions of lactobacillus bacteria, 0.1 to 2.5 portions of Torulopsis fungi, 0.1 to 2.5 portions of Aspergillus fungi, 0.1 to 2.5 portions of Rhizopus fungi, 1 to 5 portions of Penicillium fungi, 0.1 to 2.5 portions of Mucor fungi, 1 to 5 portions of Trichoderma fungi, 0.1 to 2.5 portions of Streptomycete bacteria, 1 to 5 portions of Azotobacter bacteria and 1 to 5 portions of Nitrosomonas bacteria by weight.
Preferably, the microbial compound bacteria preparation comprises 70-80 parts of bacillus bacteria, 3-8 parts of pseudomonas bacteria, 0.5-2 parts of lactobacillus bacteria, 0.5-2 parts of Torulopsis fungi, 0.5-2 parts of Aspergillus fungi, 0.5-2 parts of Rhizopus fungi, 2-4 parts of Penicillium fungi, 0.5-2 parts of Mucor fungi, 2-4 parts of Trichoderma fungi, 0.3-2 parts of Glutinosa bacteria, 2-4 parts of azotobacter bacteria and 2-4 parts of nitrosomonas bacteria by weight.
Wherein, the microbial compound bacteria preparation also comprises the following microbial flora: eremothecium (Eremothecium) fungi, Streptomyces (Streptomyces) bacteria, Neurospora (Neurospora) fungi, and Acetobacter (Acetobacter) bacteria.
Wherein, according to the weight portion, the microorganism compound bacteria preparation also comprises: 0.1-2.5 parts of eremothecium fungus, 0.1-2.5 parts of streptomyces bacteria, 0.1-2.5 parts of neurospora fungus and 0.2-3 parts of acetobacter bacteria.
Preferably, the microbial compound bacteria preparation further comprises the following components in parts by weight: 0.2-2 parts of eremothecium fungi, 0.2-2 parts of streptomyces bacteria, 0.2-2 parts of neurospora fungi and 0.5-2 parts of acetobacter bacteria.
Wherein, in the composite bacteria preparation, the weight part ratio of the fungi to the bacteria is 1: (6-15); preferably, the ratio of the fungi to the bacteria in parts by weight is 1: (7-12).
Wherein, the microbial compound bacteria preparation also comprises a filler.
Wherein the filler is a sustained release granule comprising a sugar material. Wherein the sugar substance is selected from one or more of glucose, fructose, maltose, sucrose, galactose, lactose, mannose and cellobiose.
Wherein, the raw materials of the microbial compound bacteria preparation also comprise the following components in parts by weight: and 30-50 parts of a filler.
Among them, the Bacillus bacteria include, but are not limited to, Bacillus pumilus (Bacillus pumilus), Bacillus subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus megaterium (Bacillus megaterium), Bacillus thuringiensis (Bacillus thuringiensis), Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Bacillus cereus (Bacillus cereus), Bacillus polymyxa (Bacillus polymyxa), and Bacillus Natto (Bacillus Natto).
Wherein the Penicillium fungi include, but are not limited to, Penicillium chrysogenum (Penicillium chrysogenum), Penicillium Camemberti (Penicillium Camemberti), and Penicillium lilacinum (Penicillium Luteum).
According to another aspect of the present invention, there is provided a method for preparing a microbial complex preparation, comprising the steps of:
1) respectively activating and carrying out expanded culture on bacillus bacteria, pseudomonas bacteria, lactobacillus bacteria, Torulopsis fungi, Aspergillus fungi, Rhizopus fungi, Penicillium fungi, Mucor fungi, Trichoderma fungi, Glutinosa bacteria, azotobacter bacteria and nitrosomonas bacteria to obtain expanded cultures;
2) mixing and culturing the expanded culture of Bacillus, Pseudomonas, Lactobacillus, Glutinosa, Azotobacter and Nitrosomonas bacteria until the viable bacteria concentration in the liquid fermentation liquid is not less than 1 × 109CFU/ml to obtain mixed culture 1;
3) mixing and culturing the expanded culture of Torulopsis fungus, Aspergillus fungus, Rhizopus fungus, Penicillium fungus, Mucor fungus, and Trichoderma fungus until the viable bacteria concentration in the culture solution is not less than 1 × 109CFU/ml to obtain mixed culture 2;
4) and mixing the mixed culture 1 and the mixed culture 2, and drying to obtain the microbial compound bacteria preparation.
Optionally, in step 1), adding Eremothecium fungi, Streptomyces bacteria, Neurospora fungi and Acetobacter bacteria for liquid fermentation culture.
Optionally, step (5) is further followed by step 4): the obtained microorganism complex bacteria preparation contains slow-release granules of sugar substances. The sustained-release particles containing the sugar substances are obtained by adding a mixed solution of the sugar substances, stearic acid, agar powder and polyethylene glycol into dimethyl silicone oil.
The preparation method of the sustained-release granule containing the sugar substance comprises the following steps: adding sugar substance with final concentration of 50-80g/L, stearic acid 10-30g/L, agar powder 10-20g/L and polyethylene glycol 10-30g/L (PEG5000-7000) into deionized water to prepare mixed solution; sterilizing at 100-120 deg.C for 20-40min, dripping the mixed solution into aseptic ice bath dimethyl silicone oil under aseptic condition to obtain granules with particle diameter of about 2-4mm, and absorbing dimethyl silicone oil on the surface of the granules with aseptic paper to obtain the slow release granules containing sugar substances.
According to still another aspect of the present invention, there is provided a use of the above-mentioned microbial composite bacterial preparation or the microbial composite bacterial preparation produced by the production method according to claim 8 in sewage and wastewater treatment.
In particular, the sewage or wastewater is rich in N and P.
In particular, the application of the microbial composite bacterial preparation in treatment of municipal sewage, garbage leachate wastewater, kitchen and kitchen garbage wastewater, river black and odorous water body or industrial wastewater and the like is provided.
The technical scheme of the invention has the following advantages or beneficial effects:
the active bacteria in the microbial composite bacteria preparation of the invention act synergistically, and can effectively decompose N, P, organic pollutants and other components in sewage under the action of various active enzymes or organic acids secreted by microbes. The slow release granules containing sugar substances, as a carbon substrate during periods of vigorous microbial growth, particularly in the presence of insufficient carbon source, ensure better growth and reproduction of sewage treatment microorganisms. Compared with the prior art, the microbial compound bacteria preparation contains various active bacteria, and through reasonable compatibility of various fungi and bacteria, the contents of organic matters, N, P, heavy metals and other components in sewage are obviously reduced, so that the high-efficiency purification of municipal sewage, garbage leachate wastewater, kitchen and kitchen garbage wastewater, riverway black and odorous water body or industrial wastewater is realized.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments, and it is obvious that the described embodiments are for illustrative purposes only, and not for all purposes. Based on the embodiments of the present invention, those skilled in the art will better understand and appreciate the technical solutions claimed in the present invention and the technical effects achieved thereby.
Each of the species in the examples below was purchased from a green organism.
The biological rotating disk used in the embodiment of the invention is purchased from Japan Guangen industry Co., Ltd, the material of the biological rotating disk is mainly polyvinylidene chloride, and the main working parameters are as follows: one biological rotating disc device is provided with 28 rotatable biological rotating disc plates with the diameter of 2m and the distance of 50mm, and continuously operates to treat sewage which comes from inlet water of urban sewage plants and has the treated water amount of 2800m3And/or d.
Preparation of glucose slow-release granules
Adding 60g/L glucose, 20g/L stearic acid, 15g/L agar powder and 20g/L polyethylene glycol 6000 (the content is more than or equal to 99%) into deionized water to prepare a mixed solution; sterilizing at 110 deg.C for 30min, dripping the mixed solution into aseptic ice bath dimethyl silicone oil under aseptic condition, stirring, mixing to obtain granule with particle diameter of about 2mm, and removing dimethyl silicone oil from the surface of the granule with aseptic paper to obtain glucose-containing sustained release granule.
Weighing 50 g of glucose sustained-release granules, placing the granules into 1L of deionized water, taking out every 24 hours at room temperature, drying and weighing, and observing the release trend of the granules through the weight reduction amount of the granules. The results show that the sustained-release granules prepared according to the present invention released slowly at the beginning, the release rate gradually increased with time, and released glucose slowly in a substantially even trend after 2 weeks, as shown in table 1 below.
TABLE 1
| Time (d) | Glucose concentration (g/L) |
| 1 | 0.12 |
| 2 | 0.27 |
| 3 | 0.48 |
| 4 | 0.76 |
| 5 | 1.11 |
| 6 | 1.59 |
| 7 | 2.24 |
| 8 | 2.99 |
| 9 | 3.87 |
| 10 | 4.87 |
| 15 | 9.88 |
| 20 | 15.37 |
| 25 | 20.87 |
Preparation of (II) composite bacterium preparation
Example 1
1) Respectively culturing Bacillus megaterium, Bacillus natto, Pseudomonas aeruginosa (Pseudomonas aeruginosa), Lactobacillus acidophilus (Lactobacillus acidophilus), Torulopsis histolytica (Torulopsis histolytica), Aspergillus niger (Aspergillus niger), Rhizopus nigricans (Rhizopus nigricans), Penicillium camembertis (Penicillium camembertii), Penicillium lilacinum (Penicillium Luteum), Mucor racemosus (Mucor racemosus) fungi, Trichoderma viride (Trichoderma viride), Thielavia persicum (Thiocapsa roseopisina), Azotobacter chroococcum (Azotobacter chroococcum) and Nitrosomonas europaea (Nitrosomonas europaea) in a medium comprising 5g/L of yeast extract, 10g/L of pancreatic protein and 5g/L of sodium chloride, and expanding the culture;
2) according to the weight parts of the culture, expanded cultures of 75 parts of bacillus megatherium, 1 part of bacillus natto, 5 parts of pseudomonas aeruginosa, 1 part of lactobacillus acidophilus, 0.5 part of rhododendron persicum, 3 parts of azotobacter chroococcum and 2.5 parts of nitrosomonas europaea are inoculated into a liquid culture medium 1 according to the preset weight proportion (the formula is as follows: peptone 3g/L, NaCl 5g/L, K2HPO40.3g/L, glucose 10g/L and water in balance, pH 7.5), and mixed culturing at 28 deg.C until the viable bacteria concentration of each bacterium in the liquid fermentation broth is not less than 1 × 109CFU/ml to obtain mixed culture 1;
3) expanded cultures of 1 part of histolyticum Torulopsis glabrata, 1 part of Aspergillus niger, 1 part of Rhizopus nigricans, 1 part of penicillium salmonellae, 1 part of Penicillium chrysogenum, 1 part of Mucor racemosus and 3 parts of Trichoderma viride are inoculated into a mixed culture medium 2 (the formula is as follows: sucrose 30g/L, NaNO3 2g/L、K2HPO4 1g/L、MgSO4·7H2O 0.5g/L、KCl 0.5g/L、FeSO4·7H2O0.2 g/L and water in balance, pH 7.0), mixed and cultured at 28 deg.C until the viable bacteria concentration of each bacterium in the culture solution is not less than 1 × 109CFU/ml to obtain mixed culture 2;
4) and uniformly mixing the mixed culture 1 and the mixed culture 2, and performing centrifugal separation and drying to obtain the microbial compound bacteria preparation.
Example 2
A microbial composite preparation was prepared by following the same raw materials and procedures as in example 1, wherein each bacterial culture was mixed in the following proportions.
65 parts of bacillus megaterium, 2 parts of bacillus natto, 9 parts of pseudomonas aeruginosa, 0.2 part of lactobacillus acidophilus, 2.5 parts of sulfolobus persicae, 1 part of azotobacter chroococcum, 4 parts of nitrosomonas europaea, 0.2 part of torulopsis histolytica, 2 parts of aspergillus niger, 0.1 part of rhizopus nigricans, 2 parts of penicillium salmonellae, 2 parts of penicillium flavum, 2 parts of mucor racemosus and 5 parts of trichoderma viride.
Example 3
A microbial composite preparation was prepared by following the same raw materials and procedures as in example 1, wherein each bacterial culture was mixed in the following proportions.
78 parts of bacillus megaterium, 1 part of bacillus natto, 2 parts of pseudomonas aeruginosa, 2.3 parts of lactobacillus acidophilus, 0.2 part of sulfolobus persicae, 5 parts of azotobacter chroococcum, 1 part of nitrosomonas europaea, 0.5 part of torulopsis histolytica, 0.3 part of aspergillus niger, 2.5 parts of rhizopus nigricans, 1.5 parts of penicillium sambucinna, 0.5 part of penicillium flavum, 0.2 part of mucor racemosus and 2 parts of trichoderma viride.
Example 4
A microbial composite preparation was prepared by following the same raw materials and procedures as in example 1, wherein each bacterial culture was mixed in the following proportions.
85 parts of bacillus megatherium, 2 parts of pseudomonas aeruginosa, 0.1 part of lactobacillus acidophilus, 0.1 part of sulfolobus persicae, 1 part of azotobacter chroococcum, 1 part of nitrosomonas europaea, 2 parts of torulopsis histolytica, 0.3 part of aspergillus niger, 0.5 part of rhizopus nigricans, 1 part of penicillium salmonellae, 2 parts of penicillium flavum, 0.5 part of mucor racemosus and 1.5 parts of trichoderma viride.
Example 5
A microbial composite preparation was prepared by following the same raw materials and procedures as in example 1, wherein the following fungi and bacteria were further added in the following proportions.
0.5 part of eremothecium ashbyi, 0.5 part of red bread mold, 0.5 part of streptomyces olivaceus and 1.5 parts of acetobacter aceti.
Wherein, the streptomyces olivorubidus and the acetobacter aceti are mixed and cultured in the mixed culture medium 1 in the step 2); eremothecium ashbyii and Nostoc sphaeroides were mixed-cultured in the mixed medium 2 of step 3).
Example 6
A microbial composite preparation was prepared by following the same raw materials and procedures as in example 5, wherein 40 parts of the above-prepared glucose sustained-release granules were added in step 4).
Example 7
A microbial composite preparation was prepared according to the same raw materials and procedures as in example 1, wherein 40 parts of the above-prepared glucose-releasing granule was added in step 4).
Example 8
A microbial composite preparation was prepared by following the same raw materials and procedures as in example 1, wherein Penicillium lilacinum was replaced with Penicillium chrysogenum; the lactobacillus acidophilus is replaced by lactobacillus plantarum; trichoderma viride was replaced with Trichoderma koningii.
Comparative example 1
Except that the composition only contained 75 parts of bacillus megaterium, 1 part of bacillus natto, 5 parts of pseudomonas aeruginosa, 1 part of lactobacillus acidophilus, 0.5 part of tawnia thiothrix, 3 parts of azotobacter chroococcum and 2.5 parts of nitrosomonas europaea, the composition does not contain fungi, and the rest raw materials and steps are the same as those of the embodiment 1.
Comparative example 2
The raw materials and steps were the same as in example 1 except that only 1 part of Torulopsis histolytica, 1 part of Aspergillus niger, 1 part of Rhizopus nigricans, 1 part of Penicillium camorum, 1 part of Penicillium lilacinum, 1 part of Mucor racemosus and 3 parts of Trichoderma viride were contained and no bacteria were contained.
Application example 1 municipal domestic wastewater treatment
The first step is as follows: municipal domestic sewage (raw water: CODcr 317mg/L, BOD)5＝153mg/L，TN＝43mg/L，TP＝4.6mg/L，NH3-N ═ 33mg/L) through a coarse grid well, a fine grid well and a grit chamber in sequence to remove suspended matter and impurities in the wastewater and precipitate precipitates in the water;
the second step is that: enabling the effluent of the grit chamber to flow into an intermediate water tank for mixing and adjusting water quality and water quantity, wherein the effluent of the intermediate water tank automatically flows into a biological rotating disk tank provided with a biological rotating disk device, and the hydraulic retention time is about 30 minutes;
the third step: in order to fully utilize the carbon source in the raw water, the effluent of the biological rotating disk tank flows through a first-stage denitrification tank, a first-stage nitrification tank, a second-stage denitrification tank and a second-stage nitrification tank, wherein the first-stage nitrification tank and the second-stage nitrification tank are provided with microporous aeration disks, the first-stage nitrification tank is provided with an internal reflux pump to enable nitrified liquid to reflux to the first-stage denitrification tank, and the internal reflux quantity is 1Q. Wherein in the primary nitrification tank, the water is fed at a rate of 60g/m relative to the total volume of the water fed into the biological tank3Adding the prepared microbial compound bacteria preparation into the primary nitrification tank, and then adding a microbial nutrient (Bilaiqing, Shanghai Bilaiqing Biotech limited) into the primary nitrification tank according to an instruction book by 0.5% of COD load of inlet water to ensure the flora advantage of microbial compound bacteria;
the fourth step: the effluent of the secondary nitrification tank flows into a secondary sedimentation tank, the secondary sedimentation tank carries out solid-liquid separation on the aerated mixed liquid, the precipitated sludge is pumped to an intermediate water tank by an external reflux pump arranged in an external reflux pump tank, and the reflux quantity is 0.5Q;
the fifth step: and the effluent of the secondary sedimentation tank is discharged after flowing through the disinfection system.
Separately BOD of the treated sewage5(biological oxygen demand), CODcr (chemical oxygen demand), TN, TP, NH3-N (ammonia nitrogen) is measured using industry or national standards. Wherein, the CODcr is determined by potassium dichromate method (GBT11914-89) and BOD5The measurement adopts a dilution and inoculation method (HJ 505-3The determination of-N was carried out by gas phase molecular absorption spectroscopy (HJ/T195-.
The results of the municipal sewage measurement are shown in Table 2 below:
TABLE 2
Application example 2 and landfill leachate treatment
In addition, landfill leachate (initial: CODcr 12515mg/L, BOD)5＝6511mg/L，TN＝2243mg/L，NH31983mg/L) with a water treatment capacity of 60m3And/or d.
The first step is as follows: the suspended substances and grease in the sewage are removed by an adjusting tank and an air floatation tank, the sewage is treated by the composite microbial inoculum, the CODcr value of the discharged liquid is lower than 70mg/L, the TN value is lower than 25mg/L and NH is added3-N value lower than 10mg/L, second step: the effluent of the air floatation tank is left in the mixing tank, the effluent of the mixing tank flows into a biological rotating disk tank provided with a biological rotating disk device, and the hydraulic retention time is about 1 hour;
and thirdly, in order to fully utilize the carbon source in the raw water, the effluent of the biological rotating disk tank flows through a first-stage denitrification tank, a first-stage nitrification tank, a second-stage denitrification tank and a second-stage nitrification tank, wherein the first-stage nitrification tank and the second-stage nitrification tank are provided with jet flow aerators, the first-stage nitrification tank is provided with an internal reflux pump to enable the nitrified liquid to reflux to the first-stage denitrification tank, and the internal reflux quantity is 7Q.
The fourth step: the effluent of the secondary nitrification tank flows into a secondary sedimentation tank, the secondary sedimentation tank carries out solid-liquid separation on the aerated mixed solution, most of the precipitated sludge is pumped to an intermediate water tank through an external reflux pump arranged in an external reflux pump tank, and the reflux quantity is 3Q;
the fifth step: and the effluent of the secondary sedimentation tank enters a subsequent advanced treatment unit for treatment and then is discharged.
CODcr, TN and NH treated therein with the Complex microbial preparation of examples 5-63The best removal efficiency of-N (CODcr 41mg/L, TN 28mg/L and NH)3-N ═ 6 mg/L. In contrast, the effluent treated by the composite bacterial preparation of comparative examples 1-2 can not reach GB16889-2008 'pollutant control Standard for municipal solid waste landfill'.
As can be seen from the above, the complex bacteria preparation containing both fungi and bacteria is superior to the complex bacteria preparation containing no fungi or bacteriaBOD of the complex bacterial treatment5CODcr, TN and NH3The removal effect of the-N is better, which fully indicates that the coexistence of bacteria and fungi in the compound microorganism composite bacteria preparation of the invention is beneficial to the removal of excess N, P components and suspended matters in sewage. In addition, as can be seen from examples 5 and 6, the complex microbial inoculum pair comprising a combination of Eremothecium ashbyii, Gliocladium incanus, Streptomyces olivaceus and Acetobacter aceti5CODcr, TN, and NH3The removal effect of the-N is better, probably because certain synergistic action exists among the bacteria, and the interaction among certain organic acids or active enzyme substances secreted by the bacteria promotes the rapid growth and proliferation of various bacteria, particularly bacillus, in the composite microbial inoculum, thereby better promoting the decomposition and removal of sewage pollutants.
In addition, although the presence of the glucose sustained-release particles is directed to BOD in the early stage of normal operation of the sewage or landfill leachate treatment, i.e., about 1 week after the addition of the microbial composite inoculum (as shown in Table 2 above), the glucose sustained-release particles are present5CODcr, TN, and NH3The removal of-N had no significant effect. However, with the further extension of the treatment running time, the sewage purification effect of the composite microbial inoculum containing the glucose slow-release particles is obviously better than that of the composite microbial inoculum containing no glucose slow-release particles. Specifically, in the sewage treatment of application example 1, by using the microbial composite preparation of example 7, the sewage treatment effect was still satisfactory 25 days after the addition of the microbial composite preparation containing the glucose-releasing particles, specifically, the TN removal rate was still 77% or more and NH was still present3A BOD with a-N removal rate of 91% or more5The removal rate is more than 92% and the CODcr removal rate is more than 87%; on the 25 th day of sewage treatment, the treatment efficiency of the microbial compound bacteria preparation in example 1 is reduced, specifically, the TN removal rate is still 67%, and NH is still removed3The removal rate of-N is 85%, BOD5The removal rate is 86 percent and the CODcr removal rate is 80 percent, which shows that the composite microbial inoculum containing the glucose sustained-release particles is more beneficial to sewage purification along with the prolonging of the sewage treatment time, and probably because the carbon source in the sewage is sufficient in the initial stage of sewage treatment, and the carbon source is gradually consumed along with the prolonging of the time, so that the sewage is gradually purifiedThe normal C/N ratio required by the growth of the processing microorganisms, particularly the dominant flora is broken, the further growth and proliferation of the dominant flora are seriously influenced, and the glucose slow-release particles can continuously release glucose as a carbon source substrate, so that the later nutrient requirement of the dominant flora is met, the normal metabolic activity and high activity are maintained, and the high sewage purification efficiency is maintained.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Various alternatives, modifications and combinations of the features of the invention can be made without departing from the spirit and nature of the invention as claimed, and such simple variations and combinations should also be considered as disclosed in the present application, all falling within the scope of the invention.
Claims (10)
1. The microbial compound bacterium preparation is characterized in that active ingredients of the microbial compound bacterium preparation comprise microbial floras of the following genera: bacillus, Pseudomonas, Lactobacillus, Torulopsis, Aspergillus, Rhizopus, Penicillium, Mucor, Trichoderma, Glutinosa, Azotobacter and Nitrosomonas.
2. The microbial composite bacterial preparation according to claim 1, wherein the microbial composite bacterial preparation comprises 65 to 85 parts by weight of bacillus bacteria, 2 to 10 parts by weight of pseudomonas bacteria, 0.1 to 2.5 parts by weight of lactobacillus bacteria, 0.1 to 2.5 parts by weight of torulopsis fungi, 0.1 to 2.5 parts by weight of aspergillus fungi, 0.1 to 2.5 parts by weight of rhizopus fungi, 1 to 5 parts by weight of penicillium fungi, 0.1 to 2.5 parts by weight of mucor fungi, 1 to 5 parts by weight of trichoderma fungi, 0.1 to 2.5 parts by weight of thiobacillus bacteria, 1 to 5 parts by weight of azotobacter bacteria and 1 to 5 parts by weight of nitrosomonas bacteria.
3. The microbial composite preparation according to claim 1, further comprising microbial flora of the following genera: fungi of the genus eremothecium, bacteria of the genus streptomyces, fungi of the genus neurospora, and bacteria of the genus acetobacter.
4. The microbial composite bacterial preparation according to claim 2, further comprising, in parts by weight: 0.1-2.5 parts of eremothecium fungus, 0.1-2.5 parts of streptomyces bacteria, 0.1-2.5 parts of neurospora fungus and 0.2-3 parts of acetobacter bacteria.
5. The microbial compound bacteria preparation according to claim 1, wherein the ratio of fungi to bacteria in the microbial compound bacteria preparation is 1: (6-15); preferably, the ratio of the fungi to the bacteria in parts by weight is 1: (7-12).
6. The microbial composite bacterial preparation according to claim 1, further comprising a filler comprising sustained-release particles of a sugar substance.
7. The complex microbial preparation according to claim 2, further comprising 30 to 50 parts of a filler comprising sustained-release particles of a sugar material selected from one or more of glucose, fructose, maltose, sucrose, galactose, lactose, mannose and cellobiose.
8. The method for preparing a complex microbial preparation according to any one of claims 1 to 7, comprising the steps of:
1) respectively activating and carrying out expanded culture on bacillus bacteria, pseudomonas bacteria, lactobacillus bacteria, Torulopsis fungi, Aspergillus fungi, Rhizopus fungi, Penicillium fungi, Mucor fungi, Trichoderma fungi, Glutinosa bacteria, azotobacter bacteria and nitrosomonas bacteria to obtain expanded cultures;
2) mixing expanded cultures of Bacillus, Pseudomonas, Lactobacillus, Glucothiobacillus, Azotobacter and Nitrosomonas bacteriaCo-culturing until the viable bacteria concentration of each bacterium in the liquid fermentation broth is not less than 1 × 109CFU/ml to obtain mixed culture 1;
3) mixing and culturing the expanded culture of Torulopsis fungus, Aspergillus fungus, Rhizopus fungus, Penicillium fungus, Mucor fungus, and Trichoderma fungus until the viable bacteria concentration in the culture solution is not less than 1 × 109CFU/ml to obtain mixed culture 2;
4) mixing the mixed culture 1 and the mixed culture 2, and drying to obtain a microbial compound bacteria preparation;
optionally, there is a step 5): adding filler containing slow-release particles of sugar substances into the microbial compound bacteria preparation obtained in the step 4).
9. Use of the microbial composite bacterial preparation according to any one of claims 1 to 7 or the microbial composite bacterial preparation prepared by the preparation method according to claim 8 in sewage and wastewater treatment.
10. Use according to claim 9, characterized in that the sewage or wastewater is enriched with N and P.
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