CN105176900A - Microbial preparation for urban garbage percolate treatment and application thereof - Google Patents
Microbial preparation for urban garbage percolate treatment and application thereof Download PDFInfo
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Abstract
Provided is a microbial preparation for urban garbage percolate treatment and an application thereof. The invention relates to a microbial preparation for urban sewage treatment and an application thereof. Viable bacteria in the microbial preparation comprise euryhaline bacteria bacillus cereus, Bacillus marisflavi, rhodopuesdomonas pulstris and rhodopseudomonas acidophila, Nitrosomonas europaea, marine pseudomonas and paracoccus denitrificans and yeast rhodotorula glutinis. The invention further provides a production method of the microbial preparation. According to the microbial preparation, the effect on treating garbage percolate is obvious, operation is convenient, and the microbial preparation has better application and promotion value.
Description
Technical field
The invention belongs to microbiobacterial agent scope, particularly the preparation of composite fungus agent that efficiently processes of a kind of percolate and the application in refuse treatment thereof.
Background technology
Along with the raising of human substance life horizontal, the continuous increase of city numbers and scale, domestic waste has become serious social concern.Data display according to the relevent statistics, China's rubbish average growth rate per annum reaches more than 10%, become one of country that garbage-surrounded city is the most serious in the world, in the urgent need to effectively controlling the pollution problem of domestic refuse to environment, needing badly and domestic waste being carried out effective process and disposed comprehensively.
Garbage sanitary filling disposal options is since appearance, due to possess skills reliable, technique simple, convenient management and reduced investment, working cost are low, applied widely, to component of refuse without strict demand, can the series of advantages such as ultimate disposal be made, be widely used in many countries, 70% of whole garbage treatment quantity is accounted at China's landfill disposal, and within the long duration from now on, sanitary landfill will be China's domestic rubbish disposal and the irreplaceable Main Means of disposal.But existing traditional garbage sanitary filling process disposal technology only using landfill yard as a passive system accepting rubbish, the natural degradation speed of landfill waste is very slow, landfill leachate treatment difficulty, still need in significant period of time to carry out maintenance and management to landfill yard after closing, exist and take a large amount of soil, safeguard after closing that the supervision phase is long, landfill yard uses the drawbacks such as difficulty again, does not meet the shortcomings such as the strategy of sustainable development.Therefore, reform traditional landfill process, the correlation technique of Landfill Stabilization Rate process is accelerated in research, has become extremely urgent problem.
City domestic refuse percolation liquid complicated component, pollutant concentration are high, are to carry out technological design and the comparatively thorny class polluted-water of operational management, are main pollution source of water body recognized in the world.
In China, percolate process elapsed time is more late, greatly most three phases.To the early 1990s at the bottom of the eighties, the process of percolate adopts biochemical (anoxic ten is aerobic) two step process, to remove most organic pollution materials and nitrogen, phosphorus Eutrophication materials.Due to the growth in rubbish age, naturally running of landfill yard " biochemical system ", the imbalance of carbon, nitrogen, phosphorus ratio in percolate, NH3-N too high levels in percolate, suppresses microbial growth.Mostly adopt the combination process of " materialization (lime)+stripping+biochemistry " to process percolate in subordinate phase, to solve the impact of high density NH3-N on biochemical operation, be expected under optimal operating conditions to reach secondary discharge standard, and reality was all difficult to reach secondary discharge standard in first and second improvement stage.And a large amount of lime improves pH value, need that acid is counter adjusts pH value.And lime operation labour intensity is large, pipeline very easily blocks.Present percolate process is tending towards the treatment process adopting " biochemistry+reverse osmosis (RO) ", to reach the effluent quality standard of high-quality.But the working cost of great number makes us forbidding.Certainly, many Strong oxdiative technology are still in the experimental study stage, do not carry out industrialization operation, and some external mature technologies wouldn't be applicable to the national conditions of China.From the three phases of percolate Treatment process, biochemical treatment occupies very important dominant position in each stage.Because only have biochemical treatment just can remove solubility organic pollution materials in percolate, and the material that materialization can not be removed.Therefore, adopt new processing technological flow, first carry out materializing strategy, then adopt high-performance bio microbial inoculum, extract effective Pseudomonas, strengthening biochemical effects, improving the overall effluent quality standard of percolate process, reduce pollution substance total amount, is the key point of this technological invention and enforcement.
Carbon, nitrogen, phosphorus ratio numerous imbalances in percolate, therefore the emphasis administering percolate is COD and ammonia nitrogen, adopting conventional treatment process to be difficult to the qualified discharge realizing percolate, having occurred the multiple technology and equipment for processing percolate at present.In CN1485280A patent document, such as disclose a kind of percolate utilizing submerged combustion evaporation technique to carry out landfill yard carry out treatment process, this technique is mainly by by oxidation operation being CO2 and water and the mode of carrying out evaporating and concentrate processes percolate; The technology utilizing anaerobism molecular breakdown method to carry out treating refuse percolate is disclosed in patent document CN1440941, the method comprises predecomposition step, anaerobism step, decomposes oxidation step, adsorption step, step of flocculation precipitation and filtration step, and the method combines the means of physical chemistry process and biological treatment two aspects; Similar, garbage leachate treatment process disclosed in patent document CN1478737 also uses materializing strategy and the scheme combined of carrying out a biological disposal upon, and in the process, utilizes ceramic membrane to carry out reverse-osmosis treated to the percolate through electrolytic oxidation process; A kind of process for treating percolate from garbage filling field disclosed in patent CN99124025, be by percolate with internal layer recycling mode to carrying out in the garbage loading embeading district of earthing, utilize the biological anaerobic degradation of waste layer, and then carry out aerobic degradation and membrane filtration by submerged membrane biological plant, then carry out reverse-osmosis treated; Be first adopt ammonia aeration to send out disclosed in patented invention CN1544365A, then carry out UBF anaerobic biological treatment, then through A-SBR two benches aeration, it is three grades of emission limits that the draining water quality after process can reach.
In some above-mentioned existing treatment process, especially for process ammonia nitrogen and the method adopted has and adds alkali blow-off method, chlorine break method, ion-exchange-boiling point method and biological process.But, with regard to current state of the art, add alkali blow-off method owing to suspending object height and processing cost high (20 yuan/M3) and cannot put it in waste water; Chlorine break method because of ammonia-nitrogen content high and need a large amount of chlorine and NAOH and make processing cost very high (30 yuan/M3), and the chlorine used also exists unsafe factor in storage, transport etc.; Ion-exchange-boiling point rule is only applicable to the advanced treatment of micro-ammonia nitrogen; What traditional biological denitrificaion method utilized is the nitrated of microorganism and denitrification principle, all cannot solve the technological problems that such as dissolved oxygen etc. is a lot of from theoretical and engineering practice; In addition, if ammonia nitrogen concentration is greater than 200MG/L, then the activity of nitrobacteria will be suppressed, thus reduce processing efficiency.Therefore, current refuse leachate treatment technology also exists the difficulty being much difficult to go beyond.
In prior art, activated sludge process and biomembrance process is usually adopted to perform biological treatment to percolate.Activated sludge process (oxidation ditch, SBR and plug-flow aeration tank) process operation is comparatively stable, ripe, but floor space is larger, power consumption is high, operational management is complicated, the culturing sludge time is longer, especially mud easy in inactivation between factory's turn(a)round, sewage disposal again runs mud and must again cultivate.Biomembrance process has blodisc, catalytic oxidation and BAF, and blodisc and catalytic oxidation must arrange second pond, increases soil floor space and processing cost.BAF integrates absorption, is oxidized and filters, treatment effect is good, sludge quantity is few, power consumption is low, effluent quality is good, it is the advanced technologies of current water treatment, common BAF is owing to selecting the material such as glass, pottery as filtrate, than great, therefore the maximum difficult problem run into that is in operation is that back flushing is comparatively difficult.Ammonia nitrogen too high in traditional biological treatment will produce suppression to microorganism, therefore be difficult to landfill leachate treatment to an emission standard at present.Certainly, making a final check of RO technology, reach the effluent quality of serious hope, and processing cost is bound to arouse fear.
Summary of the invention
The object of the invention is to the deficiency overcoming prior art, a kind of microbiobacterial agent percolate being had to fine treatment effect is provided.
Technical scheme of the present invention is summarized as follows:
A kind of microbiobacterial agent, it is made up of following each component: wide salt form bacterium bacillus cereus, Bacillusmarisflavi, Rhodopseudomonas palustris and rhodopseudomonas acidophilus, Nitrosomonaseuropaea, marine pseudomonas and Paracoccus denitrificans, yeast rhodotorula glutinis.
Described wide salt form bacterium bacillus cereus is the wide salt form bacterium bacillus cereus of the encoding gene having proceeded to coding any one protein variant following, described protein variant is the 59A/S that suddenlys change as follows respectively relative to albumen original amino acid, 82N/R, 100E/P, 123M/F, 139L/S, 147R/G, 179R/H, 191I/S, 203Q/K, 214A/R, albumen original amino acid represent see GenBank:WP_014597038.1(59A/S replace with S amino acid at the A amino acid of original series the 59th).Aminoacid sequence after these sudden changes makes the bacterial strain of the aminoacid sequence after having imported this sudden change have to have better effect relative to the bacterial strain do not suddenlyd change.And applicant is confirmed by a large amount of experiments, not all replacement all has similar effect, has the wide salt form bacterium bacillus cereus after a large amount of replacements not have the effect of good percolate process together with other bacterial strain.
Utilize DNAMAN software analysis, nucleotide sequence SL gene according to the synthesis of aminoacid sequence GenBank:WP_014597038.1 full genome, object fragment is obtained by carrying out amplification, pcr amplification obtains goal gene SL(and is incorporated in gene order by multiplex PCR by corresponding mutational site simultaneously, thus obtain different mutants gene), double digestion PCR primer is carried out with restriction enzyme, be connected with the same expression vector pSDK-1 through double digestion, the recombinant plasmid be proved to be successful is transformed and enters into described wide salt form bacterium bacillus cereus by the mode of the plasmid transfer commonly used by this area from intestinal bacteria, obtain the genetic engineering bacterium of efficient treating refuse percolate.
A kind of microbial preparation for the treatment of percolate of the present invention have employed salt tolerant or halophilic bacterium strain, to the strong adaptability of salinity altercation, all can grow very well in the substratum of high Cl-concentration.Compare with traditional biological treatment, the present invention has the advantages that to improve hardly degraded organic substance and ammonia nitrogen removal frank in high saliferous percolate.Experiment proves to be added in the organic waste water of supersalinity still have very high biological activity, improves the removal effect of COD and ammonia nitrogen in percolate.
Embodiment
Below in conjunction with the explanation of specific embodiment Dui Zuo Jin of the present invention mono-Walk.
Embodiment 1
Obtaining liq substratum I: take 1 part of bitter salt, 0.2 part of Repone K, 2.47 parts of sodium-chlor, 0.3 part of Trisodium Citrate, 1 part of yeast leaching powder, 0.5 part of Tryptones by quality, add water to 100 parts, regulates pH 7.0 to make liquid nutrient medium I;
Obtaining liq medium ii: take 1.2 parts of bitter salts, 0.2 part of Repone K, 2.4 parts of sodium-chlor, 0.3 part of Trisodium Citrate, 0.2 part of ammonium chloride, 0.5 part of yeast leaching powder, 0.15 part of Tryptones, 0.7 part of starch, 0.5 part of glucose by quality, add water to 100 parts, regulate pH 7.0 to make liquid nutrient medium II.
The preparation of the microbial preparation of embodiment 2 treating refuse percolate and application thereof
(1) in the liquid nutrient medium I prepared by access 1000mL embodiment 1 after wide salt form bacterium bacillus cereus activation, at 28 DEG C, 160rpm, normal pressure cultivates 36h; In the liquid nutrient medium I that gained bacterium liquid access 10L embodiment 1 is prepared, at 28 DEG C, 160rpm, normal pressure cultivates 36h; In liquid nutrient medium II gained bacterium liquid access 200L embodiment 2 prepared again, carry out scale production, at 30 DEG C, normal pressure is cultivated 48h and is obtained bacillus cereus bacteria suspension;
In the liquid nutrient medium I that after being activated by wide salt form bacterium Bacillusmarisflavi prepared by access 1000mL embodiment 1, at 28 DEG C, 160rpm, normal pressure cultivates 36h; In the liquid nutrient medium I that gained bacterium liquid access 10L embodiment 2 is prepared, at 28 DEG C, 160rpm, normal pressure cultivates 36h; In liquid nutrient medium II gained bacterium liquid access 200L embodiment 2 prepared again, carry out scale production, at 30 DEG C, normal pressure cultivates 48h, obtains Bacillusmarisflavi bacteria suspension;
Photosynthetic bacteria Rhodopseudomonas palustris and rhodopseudomonas acidophilus are activated liquid nutrient medium II prepared by rear access embodiment 1, at 25 DEG C, pH7.5, normal pressure is cultivated 36h and is obtained photosynthetic bacteria bacteria suspension;
The liquid nutrient medium II that after being activated by nitrifier Nitrosomonaseuropaea prepared by access embodiment 2, at 28 DEG C, pH8.0, oxygen supply situation 3.2mg.L
-1, normal pressure cultivate 36h obtain nitrifier bacteria suspension;
By liquid nutrient medium II prepared by access embodiment 2 after denitrifying bacteria marine pseudomonas, Paracoccus denitrificans activation, at 28 DEG C, pH7.1, leaves standstill airtight cultivation 36h and obtains denitrifying bacteria bacteria suspension;
By liquid nutrient medium n prepared by access embodiment 2 after the activation of yeast rhodotorula glutinis, at 28 DEG C, pH value 4.8, normal pressure is cultivated 32h and is obtained yeast bacteria suspension;
(2) be the ratio of 2:1:2:1:1:2 by volume, namely described Bacilluscereus bacteria suspension, Bacillusmarisflavi bacteria suspension, photosynthetic bacteria bacteria suspension, nitrifier bacteria suspension, denitrifying bacteria bacteria suspension, the mixing of yeast bacteria suspension are made a kind of microbial preparation for the treatment of percolate.
A kind of microbial preparation for the treatment of percolate of preparation is accessed the DAT section in continuum micromeehanics-continuous intermittent aeration (DAT-IAT) pond in certain refuse landfill garbage percolation liquid treating system upflow anaerobic sludge blanket process (UASB) according to 1 ‰ envelope-bulk to weight ratios, every 5 days monitoring COD, run after 20 days, add the treatment system COD clearance comparatively contradistinction system raising 20% of microbial preparation, this after-treatment system still can run 60 days in stability and high efficiency ground.
The acquisition of embodiment 3 transgenosis wide salt form bacterium bacillus cereus
Utilize DNAMAN software analysis, nucleotide sequence SL gene according to the synthesis of aminoacid sequence GenBank:WP_014597038.1 full genome, object fragment is obtained by carrying out amplification, pcr amplification obtain goal gene SL(simultaneously by multiplex PCR by corresponding mutational site 59A/S, 82N/R, 100E/P, 123M/F, 139L/S, 147R/G, 179R/H, 191I/S, 203Q/K, 214A/R is incorporated in gene order, thus obtain different mutants gene), double digestion PCR primer is carried out with restriction enzyme, be connected with the same expression vector pSDK-1 through double digestion, the recombinant plasmid be proved to be successful is transformed and enters into described wide salt form bacterium bacillus cereus by the mode of the plasmid transfer commonly used by this area from intestinal bacteria, obtain the genetic engineering bacterium of efficient treating refuse percolate.
Embodiment 4: the wastewater treatment efficiency of wide salt form bacterium bacillus cereus genetic engineering bacterium and all the other several microbial inoculums is verified
According to the method for embodiment 2, carry out corresponding percolate process experiment, the percolate of its percolate and embodiment 1 belongs to same batch, has the Pollutant levels of same concentrations.Wherein each component composition of microbial inoculum and the identical of embodiment 2.
The genetic engineering bacterium in different mutational sites is adopted to react respectively, found through experiments, 59A/S, 82N/R, 100E/P, 123M/F, 139L/S, 147R/G, 179R/H, the wide salt form bacterium bacillus cereus bacterium of this several sudden change of 191I/S, 203Q/K, 214A/R has and has relative to original bacteria wide salt form bacterium bacillus cereus the effect obviously strengthened.Its result is as follows:
As can be seen from the above results, the microbial inoculum that the wide salt form bacterium bacillus cereus adopting genetically engineered to build and other several bacterial strains are formed has the effect processing percolate preferably.
Claims (5)
1. a microbiobacterial agent, it is made up of eight kinds of microorganisms.
2. a microbiobacterial agent, it is made up of following each component: wide salt form bacterium bacillus cereus, Bacillusmarisflavi, Rhodopseudomonas palustris and rhodopseudomonas acidophilus, Nitrosomonaseuropaea, marine pseudomonas and Paracoccus denitrificans, yeast rhodotorula glutinis.
3. microbial inoculum as claimed in claim 2, is characterized in that: described wide salt form bacterium bacillus cereus is the anaerobism clostridium bacterial strain of the encoding gene having proceeded to coding any one protein variant following, and described protein variant is the 59A/S that suddenlys change as follows respectively relative to albumen original amino acid, 82N/R, 100E/P, 123M/F, 139L/S, 147R/G, 179R/H, 191I/S, 203Q/K, 214A/R, albumen original amino acid is see GenBank:WP_014597038.1.
4. the application of the microbial inoculum as described in any one of claim 1-3 in treating refuse percolate.
5. an albumen, its sequence is suddenly change as follows respectively relative to albumen original amino acid 59A/S, 82N/R, 100E/P, 123M/F, 139L/S, 147R/G, 179R/H, 191I/S, 203Q/K, 214A/R, albumen original amino acid is see GenBank:WP_014597038.1.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111439828A (en) * | 2020-04-09 | 2020-07-24 | 厦门道多生物科技有限公司 | Kitchen garbage penetrating fluid sewage treatment system |
| CN112723558A (en) * | 2020-12-16 | 2021-04-30 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate |
| CN113149326A (en) * | 2020-12-14 | 2021-07-23 | 呼伦贝尔东北阜丰生物科技有限公司 | Comprehensive utilization process of threonine mother liquor |
| CN116282574A (en) * | 2023-03-17 | 2023-06-23 | 湖南科美洁环保科技有限公司 | Efficient treatment method for landfill leachate |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434907A (en) * | 2008-12-18 | 2009-05-20 | 天津大学 | Microbial preparation for processing refuse leachate and preparation |
-
2015
- 2015-09-22 CN CN201510605543.0A patent/CN105176900A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434907A (en) * | 2008-12-18 | 2009-05-20 | 天津大学 | Microbial preparation for processing refuse leachate and preparation |
Non-Patent Citations (2)
| Title |
|---|
| NCBI: "WP_014597038.1", 《GENBANK》 * |
| 周晓黎等: "一株施氏假单胞菌Pseudomonas stutzeri DN-LWX19 的脱氮性能", 《环境工程学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111439828A (en) * | 2020-04-09 | 2020-07-24 | 厦门道多生物科技有限公司 | Kitchen garbage penetrating fluid sewage treatment system |
| CN113149326A (en) * | 2020-12-14 | 2021-07-23 | 呼伦贝尔东北阜丰生物科技有限公司 | Comprehensive utilization process of threonine mother liquor |
| CN112723558A (en) * | 2020-12-16 | 2021-04-30 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate |
| CN112723558B (en) * | 2020-12-16 | 2021-12-14 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate |
| CN116282574A (en) * | 2023-03-17 | 2023-06-23 | 湖南科美洁环保科技有限公司 | Efficient treatment method for landfill leachate |
| CN116282574B (en) * | 2023-03-17 | 2024-04-26 | 湖南科美洁环保科技有限公司 | Efficient treatment method for landfill leachate |
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Application publication date: 20151223 |