CN113149326A - Comprehensive utilization process of threonine mother liquor - Google Patents
Comprehensive utilization process of threonine mother liquor Download PDFInfo
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- CN113149326A CN113149326A CN202011462652.9A CN202011462652A CN113149326A CN 113149326 A CN113149326 A CN 113149326A CN 202011462652 A CN202011462652 A CN 202011462652A CN 113149326 A CN113149326 A CN 113149326A
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- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 239000004473 Threonine Substances 0.000 title claims abstract description 35
- 239000012452 mother liquor Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000008569 process Effects 0.000 title claims abstract description 24
- 239000003124 biologic agent Substances 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 14
- 238000004062 sedimentation Methods 0.000 claims abstract description 14
- 230000003213 activating effect Effects 0.000 claims abstract description 7
- 238000007599 discharging Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000003973 irrigation Methods 0.000 claims abstract description 7
- 230000002262 irrigation Effects 0.000 claims abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 34
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 34
- 239000000725 suspension Substances 0.000 claims description 31
- 239000001963 growth medium Substances 0.000 claims description 24
- 238000011218 seed culture Methods 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000002156 mixing Methods 0.000 claims description 20
- 241000589516 Pseudomonas Species 0.000 claims description 19
- 240000008042 Zea mays Species 0.000 claims description 18
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 18
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 18
- 229940041514 candida albicans extract Drugs 0.000 claims description 18
- 235000005822 corn Nutrition 0.000 claims description 18
- 239000012138 yeast extract Substances 0.000 claims description 18
- 238000001035 drying Methods 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 13
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 239000002131 composite material Substances 0.000 claims description 11
- 241001417524 Pomacanthidae Species 0.000 claims description 7
- 241000589755 Pseudomonas mendocina Species 0.000 claims description 7
- 241000223253 Rhodotorula glutinis Species 0.000 claims description 7
- 229960000892 attapulgite Drugs 0.000 claims description 7
- 229910052625 palygorskite Inorganic materials 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000005995 Aluminium silicate Substances 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 235000012211 aluminium silicate Nutrition 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 6
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 6
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000010902 straw Substances 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 32
- 230000001580 bacterial effect Effects 0.000 description 18
- 229910052757 nitrogen Inorganic materials 0.000 description 16
- 238000001179 sorption measurement Methods 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 229910052755 nonmetal Inorganic materials 0.000 description 5
- 239000003463 adsorbent Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- 239000002068 microbial inoculum Substances 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 235000010755 mineral Nutrition 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 230000008485 antagonism Effects 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 125000001477 organic nitrogen group Chemical group 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- -1 NO3- Chemical compound 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002734 clay mineral Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of biology, and discloses a comprehensive utilization process of threonine mother liquor, which comprises the following steps: the threonine mother liquor enters a sedimentation tank for sedimentation for 12-36 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank; taking out the biological agent, activating, then putting into a biological reaction tank, adding 10-30 g of the biological agent per cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a comprehensive utilization process of threonine mother liquor.
Background
Threonine, called Thr for short, with the chemical formula of C4H9NO3, is an essential amino acid, is the second limiting amino acid of pig feed and the third limiting amino acid of poultry feed, and gradually becomes the main limiting factor for influencing the growth of livestock and poultry along with the wide application of lysine and methionine synthetic products in compound feed. Threonine is mainly used as a feed additive, the production steps of the threonine are concentration, crystallization and extraction of mycoprotein, and mycoprotein and mother liquor generated in the process are difficult to treat and high in cost, and byproducts with high cost performance are difficult to obtain.
The amino acid fermentation mother liquor contains a large amount of total nitrogen, and is characterized in that the pollutants mainly comprise ammoniacal nitrogen, the COD content of the pollutants is higher, the content of organic matters in water is lower, and the biodegradability is poor. Total nitrogen exists in various forms, and is generally divided into inorganic nitrogen and organic nitrogen, including inorganic nitrogen such as NO3-, NO2-, and NH4+ and organic nitrogen such as protein, amino acid, and organic amine. A common sewage treatment enterprise only converts ammonia nitrogen into nitrate nitrogen to be discharged, the total nitrogen amount is not removed, the environmental problems of water eutrophication and the like cannot be reduced, only through further denitrification treatment, a carbon source is additionally added to generate nitrogen through biochemical reaction from the residual nitrate nitrogen, the nitrogen is removed from sewage, and finally harmless nitrogen discharge is realized, the ammonia nitrogen can be converted into the nitrogen only through multi-step relay required in the process, so that the process route is long, the system domestication period is long, the limiting factors are many, the occupied area of a sewage treatment tank is large, the investment cost is high, and the operation cost is large. How to stabilize and highly efficiently and deeply process the total nitrogen of threonine mother liquor is one of the problems to be solved urgently by amino acid fermentation enterprises at present.
In the prior art, physical adsorption is adopted for wastewater treatment, and an adsorption method mainly depends on the huge specific surface area of an adsorbent and removes pollutants in water through physical adsorption or chemical adsorption. The activated carbon has a rich pore structure and a huge specific surface area, has good chemical stability and strong adsorption capacity, is widely used as an important adsorbent material, but has high cost. And non-metal ion minerals such as attapulgite are used for adsorption, but natural non-metal minerals as adsorbents have the following limitations: the natural non-metal mineral has larger density and limited specific surface area, the surface of the natural non-metal mineral is negatively charged, and natural non-metal powder ore such as clay mineral is directly used as an adsorbent, so that the problem that solid and liquid are difficult to separate after adsorption exists.
In the prior patent technology of the applicant, the biological preparation for degrading organic matters in amino acid mother liquor is prepared by respectively inoculating beer yeast, rhodotorula glutinis and Angel yeast to a seed culture medium and culturing the seed culture medium until the concentration is 108cfu/ml, and then uniformly mixing to obtain a bacterial suspension; mixing the bacterial suspension and diatomite, stirring, drying at 20-22 deg.C under low temperature, drying to water content of 8-10%, and refrigerating. The biological agent is mainly used for degrading organic matters and has limited capability of degrading total nitrogen.
The strains are reasonably compatible to achieve synergistic symbiosis, and the problems can be effectively solved. However, screening for a suitable combination of agents is difficult. The composite microbial inoculum is taken as an organic whole, various strains in the microbial inoculum possibly have mutual antagonism or mutual promotion, various strains have mutual action in function, and the total technical effect is not the simple sum of the effects of all the parts. The mutual antagonism or mutual promotion of the same compound microbial inoculum in different fermentation processes can not be expected. If only the strains with similar functions or complementary strains are simply mixed, mutual antagonism among the strains is likely to occur, and the effect of the complex microbial inoculum is influenced.
Disclosure of Invention
The invention aims to provide a comprehensive utilization process of threonine mother liquor, aiming at the defects of the traditional process.
In order to realize the purpose of the invention, the following technical scheme is adopted:
a comprehensive utilization process of threonine mother liquor comprises the following steps:
the threonine mother liquor enters a sedimentation tank for sedimentation for 12-36 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank; taking out the biological agent, activating, then putting into a biological reaction tank, adding 10-30 g of the biological agent per cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
Further, the air conditioner is provided with a fan,
the process comprises the following steps: ,
mixing yeast suspension, pseudomonas suspension and carrier according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, drying to water content of 8-10%, and refrigerating at 4 ℃.
Further, the air conditioner is provided with a fan,
the preparation method of the yeast suspension comprises the following steps:
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to yeast seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain the yeast suspension.
Further, the air conditioner is provided with a fan,
the preparation method of the suspension pseudomonas suspension comprises the following steps: inoculating Pseudomonas mendocina to Pseudomonas seed culture medium, and culturing to concentration of 108cfu/ml, to obtain a Pseudomonas suspension.
Further, in the present invention,
the preparation method of the carrier comprises the following steps: taking 10 parts of attapulgite, 5 parts of corn straw powder and 3 parts of kaolin according to the parts by weight, uniformly mixing, and grinding until the particle size is 100 meshes to obtain the composite material.
Further, in the present invention,
the preparation method of the yeast seed liquid comprises the following steps:
taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract.
Further, in the present invention,
the preparation method of the pseudomonas seed culture medium comprises the following steps: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor.
The beneficial effects achieved by the invention mainly comprise but are not limited to the following aspects:
the pseudomonas belongs to non-fermentation bacteria, does not ferment saccharides, but can quickly degrade total nitrogen, and the yeast combination can effectively utilize organic matters in threonine mother liquor; the invention combines different pseudomonas and composite yeast, makes up the defect of poor capability of yeast in utilizing total nitrogen in wastewater, and finds that the combination of the pseudomonas mendocina and the composite yeast is optimal, the threonine mother liquor can be deeply repaired, the content of organic matters and total nitrogen can be rapidly reduced, and the combination mode is superior to that of other pseudomonas.
Detailed Description
Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the products and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations and modifications, or appropriate alterations and combinations, of the products and methods described herein may be made and utilized without departing from the spirit, scope, and spirit of the invention. For a further understanding of the present invention, reference will now be made in detail to the following examples.
Example 1
A comprehensive utilization process of threonine mother liquor comprises the following steps:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas mendocina to Pseudomonas seed culture medium, and culturing to concentration of 108cfu/ml to obtain a bacterial suspension B;
preparing a carrier: taking 10 parts of attapulgite, 5 parts of corn straw powder and 3 parts of kaolin according to the parts by weight, uniformly mixing, and grinding until the particle size is 100 meshes to obtain the composite material.
Mixing the bacterial suspension A, the bacterial suspension B and the carrier according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, drying to obtain a mixture with the water content of 8-10%, and finally refrigerating at 4 ℃.
The using method comprises the following steps:
the threonine mother liquor enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank;
taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
Example 2
A comprehensive utilization process of threonine mother liquor comprises the following steps:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas fluorescens to a Pseudomonas seed culture medium, and culturing to a concentration of 108cfu/ml to obtain a bacterial suspension B;
preparing a carrier: taking 10 parts of attapulgite, 5 parts of corn straw powder and 3 parts of kaolin according to the parts by weight, uniformly mixing, and grinding until the particle size is 100 meshes to obtain the composite material.
Mixing the bacterial suspension A, the bacterial suspension B and the carrier according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, drying to obtain a mixture with the water content of 8-10%, and finally refrigerating at 4 ℃.
The using method comprises the following steps:
the threonine mother liquor enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank;
taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
Example 3
A comprehensive utilization process of threonine mother liquor comprises the following steps:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas aeruginosa to a Pseudomonas aeruginosa seed culture medium, and culturing to a concentration of 108cfu/ml to obtain a bacterial suspension B;
preparing a carrier: taking 10 parts of attapulgite, 5 parts of corn straw powder and 3 parts of kaolin according to the parts by weight, uniformly mixing, and grinding until the particle size is 100 meshes to obtain the composite material.
Mixing the bacterial suspension A, the bacterial suspension B and the carrier according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, drying to obtain a mixture with the water content of 8-10%, and finally refrigerating at 4 ℃.
The using method comprises the following steps:
the threonine mother liquor enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank;
taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
Example 4
A comprehensive utilization process of threonine mother liquor comprises the following steps:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas faecalis to a Pseudomonas seed culture medium, and culturing to a concentration of 108cfu/ml to obtain a bacterial suspension B;
preparing a carrier: taking 10 parts of attapulgite, 5 parts of corn straw powder and 3 parts of kaolin according to the parts by weight, uniformly mixing, and grinding until the particle size is 100 meshes to obtain the composite material.
Mixing the bacterial suspension A, the bacterial suspension B and the carrier according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, drying to obtain a mixture with the water content of 8-10%, and finally refrigerating at 4 ℃.
The using method comprises the following steps:
the threonine mother liquor enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank;
taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
Example 5
Taking a Fufeng production workshop, threonine mother liquor (wet basis organic matter 3.12%, COD 4.26g/L and total nitrogen 389 mg/L), and treating main components in the threonine mother liquor in examples 1-4.
TABLE 1
And (4) conclusion: through verifying the utilization conditions of different embodiments on the threonine mother liquor, the embodiment 1 finds that the combination of the pseudomonas mendocina and the composite yeast selected in the embodiment is superior to the combination of other pseudomonas and the composite yeast, has better cooperative symbiosis performance, and can quickly restore the threonine mother liquor.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. The comprehensive utilization process of threonine mother liquor is characterized by comprising the following steps:
the threonine mother liquor enters a sedimentation tank for sedimentation for 12-36 hours, then the pH value is adjusted to 6-7, and then the threonine mother liquor enters a biological reaction tank; taking out the biological agent, activating, then putting into a biological reaction tank, adding 10-30 g of the biological agent per cubic meter of liquid, treating for 36-48h, filtering by a plate frame to collect microorganisms, and then discharging the liquid for field irrigation.
2. The process of claim 1, wherein the biological agent is prepared by the following method:
mixing yeast suspension, Pseudomonas mendocina suspension and carrier at a mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at 20-22 deg.C, drying to water content of 8-10%, and refrigerating at 4 deg.C.
3. The process according to claim 2, wherein the yeast suspension is prepared by:
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to yeast seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain the yeast suspension.
4. The process according to claim 2, wherein the suspension of Pseudomonas bacteria is prepared by: inoculating Pseudomonas mendocina to Pseudomonas seed culture medium, and culturing to concentration of 108cfu/ml, obtaining the Pseudomonas mendocina suspension.
5. The process according to claim 2, wherein the carrier is prepared by the method comprising: taking 10 parts of attapulgite, 5 parts of corn straw powder and 3 parts of kaolin according to the parts by weight, uniformly mixing, and grinding until the particle size is 100 meshes to obtain the composite material.
6. The process of claim 3, wherein the yeast seed culture medium is prepared by:
taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract.
7. The process of claim 4, wherein the pseudomonad seed medium is prepared by: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor.
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