CN113998832A - Method for advanced treatment of total nitrogen in amino acid wastewater - Google Patents
Method for advanced treatment of total nitrogen in amino acid wastewater Download PDFInfo
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 58
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 33
- 239000002351 wastewater Substances 0.000 title claims abstract description 32
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000003124 biologic agent Substances 0.000 claims abstract description 17
- 230000001376 precipitating effect Effects 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 26
- 238000011218 seed culture Methods 0.000 claims description 26
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 25
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 25
- 239000000725 suspension Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 229940041514 candida albicans extract Drugs 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 18
- 239000012138 yeast extract Substances 0.000 claims description 18
- 241000589516 Pseudomonas Species 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 13
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 235000005822 corn Nutrition 0.000 claims description 12
- 238000004062 sedimentation Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 241001417524 Pomacanthidae Species 0.000 claims description 7
- 241000223253 Rhodotorula glutinis Species 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 230000003213 activating effect Effects 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 6
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 241000589755 Pseudomonas mendocina Species 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 239000002068 microbial inoculum Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000008485 antagonism Effects 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 125000001477 organic nitrogen group Chemical group 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- -1 NO3- Chemical compound 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
Abstract
The invention belongs to the technical field of biology, and discloses a method for advanced treatment of total nitrogen in amino acid wastewater, which comprises the following steps: 1) preparing biological agent, 2) precipitating and adjusting pH, and 3) deeply treating. The invention can deeply repair the amino acid wastewater and quickly reduce the content of organic matters and total nitrogen.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a method for deeply treating total nitrogen in amino acid wastewater.
Background
The amino acid fermentation supplements nitrogen sources to ensure the normal metabolism of microorganisms, the wastewater generated in the post-extraction process of the fermentation liquor contains a large amount of total nitrogen, the COD content of pollutants is higher, the content of organic matters in the water is lower, and the biodegradability is poor. Total nitrogen exists in various forms, and is generally divided into inorganic nitrogen and organic nitrogen, including inorganic nitrogen such as NO3-, NO2-, and NH4+ and organic nitrogen such as protein, amino acid, and organic amine. A common sewage treatment enterprise only converts ammonia nitrogen into nitrate nitrogen to be discharged, the total nitrogen amount is not removed, the environmental problems of water eutrophication and the like cannot be reduced, only through further denitrification treatment, a carbon source is additionally added to generate nitrogen through biochemical reaction from the residual nitrate nitrogen, the nitrogen is removed from sewage, and finally harmless nitrogen discharge is realized, multiple steps required in the process can finish conversion of ammonia nitrogen into nitrogen, so that the process route is long, the system domestication period is long, the number of limiting factors is large, the occupied area of a sewage treatment tank is large, the investment cost is high, and the operation cost is large. How to stabilize and highly efficiently and deeply treat the total nitrogen of the amino acid wastewater is one of the problems to be solved urgently by amino acid fermentation enterprises at present.
In the prior patent technology of the applicant, the biological preparation for degrading organic matters in amino acid mother liquor is prepared by respectively inoculating beer yeast, rhodotorula glutinis and Angel yeast to a seed culture medium and culturing the seed culture medium until the concentration is 108cfu/ml, and then uniformly mixing to obtain a bacterial suspension; mixing the bacterial suspension and diatomite, stirring, drying at 20-22 deg.C under low temperature, drying to water content of 8-10%, and refrigerating. The biological agent is mainly used for degrading organic matters and has limited capability of degrading total nitrogen.
The strains are reasonably compatible to achieve synergistic symbiosis, and the problems can be effectively solved. However, screening for a suitable combination of agents is difficult. The composite microbial inoculum is taken as an organic whole, various strains in the microbial inoculum possibly have mutual antagonism or mutual promotion, various strains have mutual action in function, and the total technical effect is not the simple sum of the effects of all the parts. The mutual antagonism or mutual promotion of the same compound microbial inoculum in different fermentation processes can not be expected. If only the strains with similar functions or complementary strains are simply mixed, mutual antagonism among the strains is likely to occur, and the effect of the complex microbial inoculum is influenced.
Disclosure of Invention
The invention aims to provide a method for deeply treating total nitrogen in amino acid wastewater aiming at the defects of the traditional process.
In order to realize the purpose of the invention, the following technical scheme is adopted:
the method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps:
the method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps of: 1) preparing biological agent, 2) precipitating and adjusting pH, and 3) deeply treating.
Further, the 1) preparing the biological preparation comprises the following steps:
(1) preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
(2) respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
(3) preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
(4) inoculating Pseudomonas mendocina to Pseudomonas seed culture medium, and culturing to concentration of 108cfu/ml to obtain a bacterial suspension B;
(5) mixing the bacterial suspension A, the bacterial suspension B and the diatomite according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, keeping the water content of 8-10% after drying, and finally refrigerating at 4 ℃ to obtain the biological preparation.
Further, the 2) precipitation and pH adjustment:
the amino acid wastewater enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the amino acid wastewater enters a biological reaction tank.
Further, the 3) deep processing:
and (3) taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, and treating for 36-48 h.
The beneficial effects achieved by the invention mainly comprise but are not limited to the following aspects:
the pseudomonas belongs to non-fermentation bacteria, does not ferment saccharides, but can quickly degrade total nitrogen, and the yeast combination can effectively utilize organic matters in the amino acid wastewater; the invention makes up the defect of poor capability of utilizing total nitrogen in the wastewater by using yeast by combining different pseudomonas and yeast, and tests show that the combination of the pseudomonas mendocina and the composite yeast is optimal, the amino acid wastewater can be deeply repaired, the content of organic matters and total nitrogen is rapidly reduced, and the combination mode is superior to that of other pseudomonas.
Detailed Description
Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the products and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations and modifications, or appropriate alterations and combinations, of the products and methods described herein may be made and utilized without departing from the spirit, scope, and spirit of the invention. For a further understanding of the present invention, reference will now be made in detail to the following examples.
Example 1
The method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps of:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas mendocina to Pseudomonas seed culture medium, and culturing to concentration of 108cfu/ml to obtain a bacterial suspension B;
mixing the bacterial suspension A, B and diatomaceous earth at a mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 deg.C, drying to water content of 8-10%, and refrigerating at 4 deg.C.
The using method comprises the following steps:
the amino acid wastewater enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the amino acid wastewater enters a biological reaction tank;
and (3) taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, and treating for 36-48 h.
Example 2
The method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps of:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas fluorescens to a Pseudomonas seed culture medium, and culturing to a concentration of 108cfu/ml to obtain a bacterial suspension B;
mixing the bacterial suspension A, the bacterial suspension B and the diatomite according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, keeping the water content of 8-10% after drying, and finally refrigerating at 4 ℃ to obtain the biological preparation.
The using method comprises the following steps:
the amino acid wastewater enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the amino acid wastewater enters a biological reaction tank;
and (3) taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, and treating for 36-48 h.
Example 3
The method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps of:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas aeruginosa to a Pseudomonas aeruginosa seed culture medium, and culturing to a concentration of 108cfu/ml to obtain a bacterial suspension B;
mixing the bacterial suspension A, the bacterial suspension B and the diatomite according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, keeping the water content of 8-10% after drying, and finally refrigerating at 4 ℃ to obtain the biological preparation.
The using method comprises the following steps:
the amino acid wastewater enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the amino acid wastewater enters a biological reaction tank;
and (3) taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, and treating for 36-48 h.
Example 4
The method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps of:
preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
respectively inoculating beer yeast, Rhodotorula glutinis and Angel yeast to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
inoculating Pseudomonas faecalis to a Pseudomonas seed culture medium, and culturing to a concentration of 108cfu/ml to obtain a bacterial suspension B;
mixing the bacterial suspension A, the bacterial suspension B and the diatomite according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, keeping the water content of 8-10% after drying, and finally refrigerating at 4 ℃ to obtain the biological preparation.
The using method comprises the following steps:
the amino acid wastewater enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the amino acid wastewater enters a biological reaction tank;
and (3) taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, and treating for 36-48 h.
Example 5
Examples 1-4 treatment of each main component in amino acid wastewater.
TABLE 1
As can be seen from the table 1, the defect of poor capability of utilizing the total nitrogen in the wastewater by the yeast is overcome by combining different pseudomonas and composite yeast.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (4)
1. The method for deeply treating the total nitrogen in the amino acid wastewater comprises the following steps of: 1) preparing biological agent, 2) precipitating and adjusting pH, and 3) deeply treating.
2. The method of claim 1, wherein 1) preparing the biological agent comprises the steps of:
(1) preparing a yeast seed culture medium: taking 10g of yeast extract powder, 20g of peptone, 20g of glucose and 20g of agar, adding water, diluting to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the yeast extract powder;
(2) respectively separating beer yeast, Rhodotorula glutinis and Angel yeastRespectively inoculating to seed culture medium, and culturing to concentration of 108cfu/ml, and then uniformly mixing according to the equal volume proportion to obtain a bacterial suspension A;
(3) preparing a pseudomonas seed culture medium: taking 10g of yeast extract powder, 20g of corn steep liquor, 5g of ammonium chloride, 2g of dipotassium phosphate, 0.5g of magnesium sulfate heptahydrate and 0.3g of sodium chloride, adding water, fixing the volume to 1000ml, sterilizing at 121 ℃ for 20min, and naturally cooling to obtain the corn steep liquor;
(4) inoculating Pseudomonas mendocina to Pseudomonas seed culture medium, and culturing to concentration of 108cfu/ml to obtain a bacterial suspension B;
(5) mixing the bacterial suspension A, the bacterial suspension B and the diatomite according to the mass ratio of 1:1:2, stirring at 100rpm for 3min, drying at low temperature of 20-22 ℃, keeping the water content of 8-10% after drying, and finally refrigerating at 4 ℃ to obtain the biological preparation.
3. The method of claim 1 or 2, wherein the 2) precipitating and adjusting the pH:
the amino acid wastewater enters a sedimentation tank for sedimentation for 24 hours, then the pH value is adjusted to 6-7, and then the amino acid wastewater enters a biological reaction tank.
4. The method of claim 1 or 3, wherein the 3) deep processing:
and (3) taking out the biological agent, activating, then putting into a biological reaction tank, adding 20g of the biological agent into each cubic meter of liquid, and treating for 36-48 h.
Priority Applications (1)
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