CN112723558A - Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate - Google Patents
Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate Download PDFInfo
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Abstract
The invention provides application of paracoccus denitrificans in preparation of a microbial agent for degrading ammoniacal nitrogen in landfill leachate. The microbial agent comprises paracoccus denitrificans zymocyte liquid which is obtained by fermenting paracoccus denitrificans GBW-HB1904 with the preservation number of CGMCC No. 20363. The paracoccus denitrificans GBW-HB1904 disclosed by the invention has wide salt tolerance and can normally grow under the salt concentration of 25-45 per mill. The degradation rate of the paracoccus denitrificans zymocyte liquid to ammoniacal nitrogen in the high-salinity environment garbage penetrant is higher than 90%, and the biological treatment effect of ammoniacal nitrogen in high-salinity wastewater and the operation stability of a sewage treatment facility under the high-salinity extreme environment condition can be effectively improved and promoted.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and relates to application of paracoccus denitrificans in preparation of a microbial agent for degrading ammoniacal nitrogen in landfill leachate.
Background
With the improvement of living standard, a large amount of garbage is generated in production and living. At present, the garbage disposal method mainly comprises sanitary landfill, composting, incineration and the like; in the process of landfill, a large amount of landfill leachate is generated, and the composition of the landfill leachate is complex and mainly depends on the composition of the garbage, the particle size of the garbage, the compaction degree, the climate on the site, the hydrological conditions, the landfill time and the like. The landfill leachate belongs to high-concentration organic wastewater, the COD content is generally higher, but besides COD, ammoniacal nitrogen is also a conventional discharge index required to be controlled in the treatment process of the landfill leachate. The ammonia nitrogen in the percolate mainly comes from biodegradation of nitrogenous compounds such as protein and the like in the landfill garbage, and the concentration change of the ammonia nitrogen tends to rise along with the extension of the landfill time. The ammonia nitrogen content of the landfill leachate is too high, so that the ground water body is anoxic and the water quality is deteriorated; the nutrient substances such as nitrogen and phosphorus are the inducement of water eutrophication, so that the water source of drinking water can be seriously influenced, and the research on the removal of ammoniacal nitrogen in the landfill leachate has important significance on the treatment of the leachate.
However, since some coastal areas have applied seawater to industrial production and living, and materials such as pesticides, herbicides, organic peroxides, pharmaceuticals, dyes, etc. used in chemical manufacturing, meat processing plants, marine product processing plants, etc., landfill leachate contains a large amount of inorganic salts, resulting in high salinity of the leachate. However, these high salinity permeates have a strong inhibitory effect on most of the microorganisms used to treat the landfill permeate. Therefore, the strains or the microbial inoculum which is salt-tolerant or halophilic and can play a positive role in the treatment of the high-salt-content penetrating fluid has great significance for the treatment of the landfill leachate in the future.
Disclosure of Invention
The invention provides application of paracoccus denitrificans in preparation of a microbial agent for degrading ammoniacal nitrogen in landfill leachate, and the paracoccus denitrificans GBW-HB1904 has good salt resistance and can efficiently degrade ammoniacal nitrogen in high-salt landfill leachate.
In order to achieve the purpose of the invention, the invention is realized by the following technical scheme:
the invention provides application of paracoccus denitrificans in preparation of a microbial agent for degrading ammoniacal nitrogen in landfill leachate.
Further, the microbial agent comprises paracoccus denitrificans zymocyte liquid.
Further, the paracoccus denitrificans zymocyte liquid is obtained by fermenting paracoccus denitrificans GBW-HB1904 with the preservation number of CGMCC No. 20363.
Further, the paracoccus denitrificans GBW-HB1904 has wide salt tolerance.
Furthermore, the growth salinity range of the paracoccus denitrificans GBW-HB1904 is 25-45 per mill.
Further, the fermentation conditions of the paracoccus denitrificans GBW-HB1904 are as follows: the tank pressure is 0.1-0.2 MPa, the temperature is 28-32 ℃, the dissolved oxygen is more than or equal to 30%, the stirring speed is 180-200 rpm, and the fermentation time is 20-24 h.
Furthermore, the growth temperature of the paracoccus denitrificans GBW-HB1904 is 15-40 ℃, and the optimal growth temperature is 28-32 ℃.
Furthermore, the growth pH value of the paracoccus denitrificans GBW-HB1904 is 6-9, and the optimal growth pH value is 7.0-8.0.
Furthermore, paracoccus denitrificans GBW-HB1904 is facultative anaerobe, and the concentration of dissolved oxygen for growth is 0.2-4 mg/L.
Further, the paracoccus denitrificans GBW-HB1904 enters a logarithmic growth phase after being cultured for 5 hours, enters a last logarithmic growth phase after 18-20 hours, and the bacterial count reaches 1 multiplied by 1010CFU/mL。
Further, the culture medium formula for the suitable growth of paracoccus denitrificans GBW-HB1904 comprises the following components: 50g/L glucose, 10g/L, NaCl 5g/L, NaNO g corn steep liquor dry powder3 2g/L、KH2PO4 0.2g/L、FeSO4·7H2O 0.05g/L、Na2HPO4 5g/L、MgSO4·7H2O 0.1g/L。
Further, when the biological treatment system is applied, the addition amount of the paracoccus denitrificans zymocyte liquid is 1-3 per mill of the volume of an aerobic pool in the garbage penetrating liquid biochemical treatment system.
Further, the bacterial content of paracoccus denitrificans GBW-HB1904 in the paracoccus denitrificans zymocyte liquid is not less than 1.0 multiplied by 1010CFU/mL。
Furthermore, the degradation rate of the paracoccus denitrificans zymocyte liquid on ammoniacal nitrogen in the landfill leachate is higher than 90%.
Further, the microbial agent comprises liquid, powder and granular.
Compared with the prior art, the invention has the following advantages and technical effects:
the paracoccus denitrificans GBW-HB1904 disclosed by the invention is separated from a high-salt water environment, has wide salt tolerance and can grow well within a salinity range of 25-45%. Paracoccus denitrificans GBW-HB1904 is cultured in vitro and propagated quickly, and can reach the late logarithmic phase within 18-20 h; in addition, the bacteria have the capability of rapidly degrading ammoniacal nitrogen in the landfill leachate in a high-salinity environment, and the degradation rate can reach more than 90 percent, so that the bacteria can be used for developing products for removing ammoniacal nitrogen in the high-salinity landfill leachate and applied to the treatment of high-salinity wastewater. The paracoccus denitrificans GBW-HB1904 is simple in use method, only needs to be prepared into zymocyte liquid and directly added into a penetrating fluid treatment system, can effectively solve the problems of poor microbial biochemical property, low pollutant removal efficiency and the like of a high-salinity sewage system, can effectively improve and promote the biological treatment effect of ammoniacal nitrogen in high-salinity wastewater and the operation stability of sewage treatment facilities under high-salinity extreme environmental conditions, and has wide application prospect and value.
Drawings
FIG. 1 is a colony morphology of the paracoccus denitrificans GBW-HB1904 on a modified NB solid medium plate.
FIG. 2 is a growth curve of the Paracoccus denitrificans GBW-HB 1904.
FIG. 3 shows the influence of the paracoccus denitrificans GBW-HB1904 on the degradation rate of ammoniacal nitrogen in landfill leachate.
Detailed Description
The technical solution of the present invention will be further described in detail with reference to the following specific examples.
In the following examples, unless otherwise specified, the experimental methods used were all conventional methods, and materials, reagents and the like used were all available from biological or chemical reagents companies.
The formulations of the media required in the examples are as follows:
1. enriching and screening a liquid culture medium by using high-salinity nitrate nitrogen degrading bacteria: glucose 10g, NaNO3 2g，Na2CO3 1.5g，K2HPO4 1.0g，MgSO4·7H2O 0.05g，FeSO4·7H20.5g of O, 40g of NaCl, 7.0-8.0 of pH and 1L of distilled water.
2. Separating and purifying the solid culture medium by the high-salinity nitrate nitrogen degrading bacteria: glucose 10g, NaNO3 2g，Na2CO3 1.5g，K2HPO4 1.0g，MgSO4·7H2O 0.05g，FeSO4·7H20.5g of O, 40g of NaCl, 15g of agar powder, 7.0-8.0 of pH and 1L of distilled water.
3. Modified Nutrient Broth (NB) medium: beef extract 5g, peptone 10g, sodium chloride 5g, NaNO3 2g/L、FeSO4·7H2O 0.05g/L、MgSO4·7H20.1g/L of O and 1L, pH 7.0.0-7.5 of water.
4. Modified Nutrient Broth (NB) solid medium: beef extract 5g, peptone 10g, sodium chloride 5g, NaNO3 2g/L、FeSO4·7H2O 0.05g/L、MgSO4·7H20.1g/L of O, 15g of agar powder and 1L, pH 7.0.0-7.5 of water.
5. Fermentation medium: 50g/L glucose, 10g/L, NaCl 5g/L, NaNO g corn steep liquor dry powder3 2g/L、KH2PO40.2g/L、FeSO4·7H2O 0.05g/L、Na2HPO4 5g/L、MgSO4·7H20.1g/L of O, the balance of water, and the pH value of the liquid culture medium is 7.0-7.5.
The above culture medium is sterilized at 121 deg.C for 20min before use, and then stored at room temperature.
Example 1: screening, separation and identification of paracoccus denitrificans GBW-HB1904
1. Screening and purifying paracoccus denitrificans GBW-HB1904
Taking 5g of landfill leachate and food factory pickled wastewater respectively, adding into 100mL of PBS buffer solution, oscillating for 30min to fully mix the samples, centrifuging at 1000rpm for 2min, and collecting the sample supernatant for later use. And (3) adding 5mL of the sample supernatant into conical flasks filled with 100mL of the high-salinity nitrate nitrogen degrading bacteria enrichment screening liquid culture medium, and carrying out shaking culture at 30 ℃ and 160rpm for 2d for enrichment 3 times. After the cultured bacterial suspension is diluted in a gradient way, 100 mu L of the bacterial suspension is coated on a solid culture medium for enriching and screening the high-salinity nitrate nitrogen degrading bacteria and is cultured in an incubator at 30 ℃. After 48h, single colonies of different morphologies were picked and streaked on modified Nutrient Broth (NB) solid medium, and isolation and purification were repeated 3 times to obtain a single colony, which was named as GBW-HB 1904.
As shown in figure 1, the bacterial colony of the bacterial strain GBW-HB1904 on a modified Nutrient Broth (NB) solid medium plate is circular, milk white, 0.2-0.4mm in diameter, smooth, moist and glossy in surface, slightly convex in middle, opaque, neat in edge and free of halo.
2. Identification of Paracoccus denitrificans GBW-HB1904
The DNA of the strain GBW-HB1904 is used as a template, 16S rRNA universal primers are used for amplification, the sequence of an amplified fragment is determined, the 16S rDNA sequencing result of the obtained strain GBW-HB1904 is compared with the sequence in GenBank for analysis, and the result shows that the homology of the strain GBW-HB1904 and Paracoccus denittificans is the highest, so that the strain GBW-HB1904 is determined to be Paracoccus denitrificans.
And (3) performing strain preservation on the screened strain GBW-HB1904, wherein the preservation unit of paracoccus denitrificans GBW-HB 1904: china general microbiological culture Collection center (CGMCC); address: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: year 2020, month 07, 15; the deposit number of Paracoccus denitrificans Paracoccus dentiricans is as follows: CGMCC No. 20363.
Example 2: growth determination and physiological and biochemical characteristics of paracoccus denitrificans GBW-HB1904
1. Growth assay for Paracoccus denitrificans GBW-HB1904
Inoculating paracoccus denitrificans GBW-HB1904 cultured by a slant to an improved NB culture medium, performing shake culture at the constant temperature of 28-32 ℃ for 26h at the pH of 7.0-7.5 to prepare GBW-HB1904 bacterial liquid, sampling every 1.0h, measuring the light absorption value at OD600 nm, and drawing a growth curve. As shown in FIG. 2, the experimental results show that GBW-HB1904 is in the growth retardation phase in the first 5h of culture, then enters the logarithmic growth phase, and enters the terminal logarithmic growth phase when being cultured for 18-20h, and the bacterial count reaches 1.0 × 1010CFU/mL, 20-24h for the growth stationary phase, followed by the decline phase, completing the entire growth cycle.
2. Physiological and biochemical characteristics of paracoccus denitrificans GBW-HB1904
The prepared GBW-HB1904 bacterial liquid is further cultured in an NB medium, and then the paracoccus denitrificans GBW-HB1904 is detected according to a strain physiological and biochemical detection method of Bergey bacteria identification manual. As shown in Table 1, Paracoccus denitrificans GBW-HB1904 can normally grow at a temperature of 15-40 ℃, and the optimal growth temperature is 28-32 ℃; can grow in the pH value range of 6-9, and the optimum growth pH value is 7.0-8.0; the paracoccus denitrificans GBW-HB1904 belongs to facultative anaerobes, and can normally grow within the dissolved oxygen content of 0.2-4 mg/L, the optimal growth dissolved oxygen concentration is 1-3 mg/L; the strain can produce urease, beta-glucosidase and glycerol, and can degrade power-nitrate, simon citrate and semi-solid agar. In addition, the medium formulation suitable for growth of Paracoccus denitrificans GBW-HB1904 is as follows: 50g/L glucose, 10g/L, NaCl 5g/L, NaNO g corn steep liquor dry powder3 2g/L、KH2PO4 0.2g/L、FeSO4·7H2O 0.05g/L、Na2HPO4 5g/L、MgSO4·7H20.1g/L of O, the balance of water, and the pH value of the liquid culture medium is 7.0-7.5.
TABLE 1 physio-biochemical characteristics of Paracoccus denitrificans GBW-HB1904
Note: < + > represents positive, and < - > represents negative.
Example 3: salinity tolerance test of Paracoccus denitrificans GBW-HB1904
In order to examine the survival performance of paracoccus denitrificans GBW-HB1904 under high salinity conditions, the growth of the paracoccus denitrificans GBW-HB1904 under different salinity conditions was analyzed and detected. Adjusting the amount of sodium chloride in the NB medium to prepare culture media with different salt concentrations, inoculating paracoccus denitrificans GBW-HB1904 cultured by a slant to the NB medium with different salt concentrations, culturing for 20h in a constant temperature shaking table with the pH of 7.0-7.5 and the temperature of 30 ℃ to obtain a paracoccus denitrificans GBW-HB1904 culture solution, and detecting the growth conditions of the paracoccus denitrificans in the culture media with different salt concentrations. As shown in Table 2, GBW-HB1904 shows broad salt tolerance and can grow well in a salinity range of 25 to 45 ‰.
TABLE 2 growth of Paracoccus denitrificans GBW-HB1904 in different salt concentrations
Example 4: ammonia nitrogen removal test under high salt conditions
Inoculating paracoccus denitrificans GBW-HB1904 cultured by a slant to an improved NB culture medium, and culturing for 20h in a constant-temperature shaking table with the pH of 7.0-7.5 and the temperature of 30 ℃ to obtain paracoccus denitrificans GBW-HB1904 seed solution; inoculating paracoccus denitrificans GBW-HB1904 seed liquid into a fermentation medium according to the volume ratio of 10%, adjusting the tank pressure to be 0.1MPa, the temperature to be 30 ℃, the dissolved oxygen to be more than or equal to 30%, the stirring speed to be 200rpm, fermenting for 20h to obtain paracoccus denitrificans GBW-HB1904 zymocyte liquid, wherein the bacterium content in the zymocyte liquid is not less than 1.0 multiplied by 1010CFU/mL。
And (3) detecting the ammoniacal nitrogen concentration of the water body by adopting a Neel's reagent colorimetric method (GB-7479.87). Under the condition of simulating high salinity (salinity is 40 per mill, pH is 7.5, temperature is 28 ℃, and dissolved oxygen is more than or equal to 2mg/L), paracoccus denitrificans GBW-HB1904 is used for treating 48h ammonia nitrogen removal application experiments of water bodies containing ammonia nitrogen with different concentrations, the ammonia nitrogen removal rate is calculated, and 3 repeated experiments are set for each concentration gradient.
60mL of paracoccus denitrificans GBW-HB1904 zymocyte liquid is taken for centrifugation, 15mL of sterile water is added for re-suspending and uniformly mixing the precipitated thalli, 1mL of bacterial suspension is taken and respectively added into 5 test liquids with different initial ammonia nitrogen concentrations, the system of which is 100mL, and the test of 48h ammonia nitrogen removal rate is carried out. The results are shown in Table 3, after 48h treatment, paracoccus denitrificans GBW-HB1904 has obvious removal effect on ammonia nitrogen with different initial concentrations under the high salinity condition, and the removal rate is over 90 percent, which indicates that the strain has the capability of quickly and effectively removing ammonia nitrogen in water under the high salinity condition, and has great application prospect and value in the aspect of removing ammonia nitrogen in high salinity wastewater.
TABLE 3 48h Ammonia Nitrogen removal test results of Paracoccus denitrificans GBW-HB1904
Example 5: evaluation of paracoccus denitrificans GBW-HB1904 on removal rate of ammonia nitrogen in landfill leachate
The method comprises the steps of collecting a sewage water sample in an aerobic pool of a certain landfill leachate sewage treatment biochemical system in Tianjin, equally and separately loading sewage into 8 wide-mouth beakers of 2L, wherein each beaker is separately loaded with 1.5L of leachate wastewater and is totally divided into 4 groups, each group is 2 in parallel, a CK group is a blank control group without adding paracoccus denitrificans GBW-HB1904 zymogen liquid, other test conditions are the same as those of the test groups, the test groups are S1, S2 and S3 groups, and the addition amount of the zymogen liquid corresponding to each group is 1 per thousand, 2 per thousand and 3 per thousand respectively. The initial concentration of ammoniacal nitrogen in each group of samples is reserved and detected before the test is started, the pH value of each group is controlled to be 6-9, each group is aerated by using a small aerator with the same model, the dissolved oxygen in water of each group is controlled to be more than or equal to 2mg/L, the pH value and the ammoniacal nitrogen concentration in each group of samples are tracked and detected at intervals of 6 hours, the degradation rate of ammoniacal nitrogen in a water sample is analyzed, and the whole test period is 48 hours. The test results are shown in fig. 3, compared with a blank control group, the test groups can obviously improve the degradation rate of ammoniacal nitrogen in a water sample, the final degradation rate is over 90%, and in addition, the test groups with different zymophyte liquid added have no obvious difference in the aspect of improving the degradation rate of ammoniacal nitrogen, which shows that the paracoccus denitrificans GBW-HB1904 zymophyte liquid also has the capability of efficiently degrading ammoniacal nitrogen in landfill leachate under the condition of low addition amount.
The paracoccus denitrificans GBW-HB1904 has stable property, wide salt resistance and simple and convenient application, and can be prepared into paracoccus denitrificans GBW-HB1904 with the bacterium content of not less than 1.0 multiplied by 1010After the fermentation broth is CFU/mL, the fermentation broth is directly added into a sewage biochemical treatment system from a water inlet of an aerobic tank according to the use proportion of 1-3 per mill of the volume of the aerobic tank in the biochemical treatment system, and the bacteria can particularly and obviously improve the removal efficiency of ammoniacal nitrogen in garbage penetrating fluid, so the bacteria have wide market prospect and industrial value.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (9)
1. Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate.
2. The use of claim 1, wherein the microbial agent comprises paracoccus denitrificans zymogen liquid.
3. The use of claim 2, wherein the paracoccus denitrificans zymocyte solution is obtained by fermenting paracoccus denitrificans GBW-HB1904 with the preservation number of CGMCC No. 20363.
4. The use according to claim 3, wherein the paracoccus denitrificans GBW-HB1904 has broad salt tolerance.
5. The use according to claim 4, wherein the paracoccus denitrificans GBW-HB1904 has a growth salinity ranging from 25 to 45% o.
6. The use according to claim 3, wherein the fermentation conditions of paracoccus denitrificans GBW-HB1904 are: the tank pressure is 0.1-0.2 MPa, the temperature is 28-32 ℃, the dissolved oxygen is more than or equal to 30%, the stirring speed is 180-200 rpm, and the fermentation time is 20-24 h.
7. The use of claim 2, wherein the addition amount of the paracoccus denitrificans zymocyte liquid is 1-3 per mill of the volume of an aerobic pool in the garbage leachate biochemical treatment system.
8. The use of claim 3, wherein the bacterial content of Paracoccus denitrificans GBW-HB1904 in the paracoccus denitrificans fermented liquid is not less than 1.0 x 1010CFU/mL。
9. The use of claim 3, wherein the paracoccus denitrificans zymocyte liquid has a degradation rate of ammoniacal nitrogen in landfill leachate higher than 90%.
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| CN113830902A (en) * | 2021-09-23 | 2021-12-24 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater |
| KR20230039435A (en) * | 2021-09-14 | 2023-03-21 | 코스맥스 주식회사 | Scalp microbiome complex and its use for improving hair or scalp condition |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176900A (en) * | 2015-09-22 | 2015-12-23 | 宁波创蓝环境科技有限公司 | Microbial preparation for urban garbage percolate treatment and application thereof |
| US20160167993A1 (en) * | 2012-06-15 | 2016-06-16 | Microvi Biotech, Inc. | Biocatalyst compositions and processes for use |
| CN106635858A (en) * | 2015-11-04 | 2017-05-10 | 中国石油化工股份有限公司 | Paracoccus sp. and culture application thereof |
| CN107090418A (en) * | 2017-05-17 | 2017-08-25 | 武汉科缘生物发展有限责任公司 | One strain denitrogen paracoccus and its application in livestock and poultry farm wastewater treatment |
-
2020
- 2020-12-16 CN CN202011490450.5A patent/CN112723558B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160167993A1 (en) * | 2012-06-15 | 2016-06-16 | Microvi Biotech, Inc. | Biocatalyst compositions and processes for use |
| CN105176900A (en) * | 2015-09-22 | 2015-12-23 | 宁波创蓝环境科技有限公司 | Microbial preparation for urban garbage percolate treatment and application thereof |
| CN106635858A (en) * | 2015-11-04 | 2017-05-10 | 中国石油化工股份有限公司 | Paracoccus sp. and culture application thereof |
| CN107090418A (en) * | 2017-05-17 | 2017-08-25 | 武汉科缘生物发展有限责任公司 | One strain denitrogen paracoccus and its application in livestock and poultry farm wastewater treatment |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230039435A (en) * | 2021-09-14 | 2023-03-21 | 코스맥스 주식회사 | Scalp microbiome complex and its use for improving hair or scalp condition |
| WO2023043111A1 (en) * | 2021-09-14 | 2023-03-23 | 코스맥스 주식회사 | Scalp microbiome complex and use thereof for improving hair or scalp condition |
| KR102558084B1 (en) | 2021-09-14 | 2023-07-21 | 코스맥스 주식회사 | Scalp microbiome complex and its use for improving hair or scalp condition |
| CN113830902A (en) * | 2021-09-23 | 2021-12-24 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater |
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