CN112592853A - Microbial agent containing alcaligenes faecalis and application thereof - Google Patents

Microbial agent containing alcaligenes faecalis and application thereof Download PDF

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CN112592853A
CN112592853A CN202011490779.1A CN202011490779A CN112592853A CN 112592853 A CN112592853 A CN 112592853A CN 202011490779 A CN202011490779 A CN 202011490779A CN 112592853 A CN112592853 A CN 112592853A
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alcaligenes faecalis
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microbial agent
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王金龙
王熙涛
袁绍辉
赵金超
张晓春
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Qingdao Shangde Biotechnology Co ltd
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    • C02F2101/16—Nitrogen compounds, e.g. ammonia
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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract

The invention provides a microbial agent containing alcaligenes faecalis and application thereof. The microbial agent comprises the microbial content of not less than 5.0 x 108CFU/g of alcaligenes faecalis powder and a carrier, wherein the mass ratio of the alcaligenes faecalis powder to the carrier is 1: 50-100. The Alcaligenes faecalis powder is prepared from Alcaligenes faecalis GBW-HB190 with the preservation number of CGMCC No.203645, the strain has broad-salt tolerance, and the growth salinity range is 15-35 per mill. The microbial inoculum has the ability of rapidly and efficiently removing COD, ammonia nitrogen and total nitrogen in slaughter wastewater, thereby further improving the treatment efficiency of COD, ammonia nitrogen and total nitrogen in the wastewater, improving the operation stability of a wastewater treatment system and ensuring that the effluent index is stable and reaches the standard.

Description

Microbial agent containing alcaligenes faecalis and application thereof
Technical Field
The invention belongs to the technical field of microorganisms, and relates to a microbial agent containing alcaligenes faecalis and application thereof.
Background
Along with the economic development and the continuous improvement of living standard in recent years, the living demand of people on meat products is continuously increased, the operation scale of each slaughterhouse is continuously expanded, the slaughter industry reaches unprecedented expansion and development, and the wastewater discharge amount is also continuously increased. Wherein, the slaughter wastewater mainly comprises slaughter industries from livestock raising, poultry, fishes and the like. According to statistics, at present, the number of pig killing industries in China is more than 1000, the total annual discharged wastewater amount reaches about 3 billion cubic meters, and the discharged wastewater amount accounts for about 6 percent of national industrial wastewater discharge amount. If the waste water is directly discharged without being treated, the environmental protection supervision department can cause serious pollution to the surrounding water environment and great harm to the health of people and livestock, so that the environmental protection supervision department can forcibly require that a special waste water treatment station must be established in a slaughterhouse to treat the waste water generated in the slaughtering process.
The slaughtering wastewater has obvious characteristics that firstly, the slaughtering wastewater has very unpleasant fishy smell, secondly, the slaughtering wastewater is generally red brown, and the wastewater also contains a large amount of blood, grease, animal internal organs, fur, meat scraps, undigested food in animal stomach, excrement and the like, thereby seriously polluting and influencing social environment. The main pollution factors contained in slaughtering wastewater comprise COD, ammonia nitrogen, grease, total nitrogen and the like, and the treatment of the wastewater is one of the problems of great concern and headache in China.
Therefore, in the process of treating the wastewater by using a biochemical treatment mode, finding microbial strains which can efficiently degrade and remove COD, ammonia nitrogen and total nitrogen in the wastewater becomes one of the key factors influencing the standard-reaching harmless treatment of the wastewater.
Disclosure of Invention
The invention provides a microbial agent containing alcaligenes faecalis and application thereof. The microbial agent comprises bacillus foecalis alkaligenes GBW-HB1905 bacterial powder and a carrier, and has the function of efficiently removing COD, ammonia nitrogen and total nitrogen in slaughter wastewater.
In order to achieve the purpose of the invention, the invention is realized by the following technical scheme:
the invention provides a microbial agent containing alcaligenes faecalis, which comprises a microbial agent with the bacterium content of not less than 5.0 multiplied by 108CFU/g Alcaligenes faecalis powder and a carrier.
Further, the mass ratio of the alcaligenes faecalis powder to the carrier is 1: 50-100.
Further, the alcaligenes faecalis powder is prepared from alcaligenes faecalis GBW-HB1905 with the preservation number of CGMCC No. 20364.
Further, the preparation method of the alcaligenes faecalis powder comprises the following steps:
(1) inoculating the Alcaligenes faecalis GBW-HB1905 into an improved NB culture medium, and culturing for 22h in a constant-temperature shaking table with the pH of 7.5-8.5 and the temperature of 28 ℃ to obtain an Alcaligenes faecalis GBW-HB1905 seed solution;
(2) inoculating 8-10% of the Alcaligenes faecalis GBW-HB1905 seed solution into a fermentation medium according to the volume ratio, adjusting the tank pressure to 0.1-0.2 MPa, the temperature to 28-30 ℃, the dissolved oxygen to be not less than 25%, stirring at 160-180 rpm, and fermenting for 16-20h to obtain an Alcaligenes faecalis GBW-HB1905 zymocyte solution;
(3) mixing the bacillus alcaligenes faecalis GBW-HB1905 zymocyte liquid and a carrier according to the volume-mass ratio of 1: 2-4, and carrying out vacuum freeze drying at the temperature of-40 to-50 ℃ to obtain bacillus alcaligenes faecalis GBW-HB1905 bacterial powder.
Furthermore, the Alcaligenes faecalis GBW-HB1905 has broad salt tolerance, and the growth salinity range is 15-35 per mill.
Furthermore, the growth temperature of the Alcaligenes faecalis GBW-HB1905 is 15-40 ℃, and the optimal growth temperature is 25-32 ℃.
Further, the growth pH value of the Alcaligenes faecalis GBW-HB1905 is 6-9, and the optimum pH value is 7.5-8.5.
Furthermore, the Alcaligenes faecalis GBW-HB1905 can efficiently degrade COD, ammonia nitrogen and total nitrogen in pollutants in slaughter wastewater.
Furthermore, the Alcaligenes faecalis GBW-HB1905 has a high propagation speed, enters a logarithmic growth phase after being cultured for 4 hours, enters a terminal logarithmic growth phase after being cultured for 14-16 hours, and the number of bacteria reaches 6.0 multiplied by 109CFU/mL。
Further, suitable growth media for the Alcaligenes faecalis GBW-HB1905 include glucose, yeast powder, peptone and corn steep liquor dry powder.
Further, the carrier is at least one of calcium carbonate, talcum powder and diatomite.
The invention also provides application of the microorganism bacterium agent containing the alcaligenes faecalis in preparing a preparation for degrading pollutants in slaughter wastewater.
Further, the use method of the microbial agent comprises the following steps: the microbial agent is directly added into an anoxic tank and an aerobic tank, the dissolved oxygen in the water of the aerobic tank is kept to be more than or equal to 2mg/L, the dissolved oxygen in the water of the anoxic tank is kept to be less than or equal to 0.5mg/L, and the pH values of the anoxic tank and the aerobic tank are controlled to be 7.0-8.0.
Furthermore, the adding concentrations of the microbial inoculum in the anoxic tank and the aerobic tank of the slaughter wastewater treatment system are respectively 80-100ppm and 180-200 ppm.
Further, the pollutants include COD, ammonia nitrogen and total nitrogen.
Furthermore, the microbial agent can control the COD concentration in the effluent of the slaughtering wastewater to be below 30mg/L, the ammonia nitrogen concentration to be below 2mg/L and the total nitrogen concentration to be below 7 mg/L.
Compared with the prior art, the invention has the following advantages and technical effects:
the Alcaligenes faecalis GBW-HB1905 disclosed by the invention is separated from a water body environment containing high-salinity wastewater, has a salt-tolerant growth characteristic, and can grow well within a salinity range of 15-35 per thousand. The microbial agent can be prepared by mixing bacterial powder obtained by culturing and fermenting the alcaligenes faecalis GBW-HB1905 with a carrier. The microbial agent containing the alcaligenes faecalis GBW-HB1905 has the capability of quickly degrading COD, ammonia nitrogen and total nitrogen in slaughter wastewater, and can control the COD concentration in the effluent of the slaughter wastewater to be below 30mg/L, the ammonia nitrogen concentration to be below 2mg/L and the total nitrogen concentration to be below 7 mg/L. Therefore, the microbial agent can be used for developing products for removing COD, ammonia nitrogen and total nitrogen in slaughter wastewater, can further improve the treatment efficiency of COD, ammonia nitrogen and total nitrogen in the wastewater treatment process, improves the operation stability of a wastewater treatment system, ensures that the effluent index is stable and reaches the standard, and has important application value and significance.
Drawings
FIG. 1 is a colony morphology of the Alcaligenes faecalis GBW-HB1905 on modified Nutrient Broth (NB) solid medium plates.
FIG. 2 is a growth curve of the Alcaligenes faecalis GBW-HB 1905.
FIG. 3 shows the effect of the Bacillus foecalis alkaligenes GBW-HB1905 bacterial agent on COD concentration in slaughter wastewater.
FIG. 4 shows the influence of the Alcaligenes faecalis GBW-HB1905 on the ammonia nitrogen concentration in slaughter wastewater.
FIG. 5 is a graph showing the effect of the Alcaligenes faecalis GBW-HB1905 microbial inoculum on the total nitrogen concentration in slaughter wastewater.
Detailed Description
The technical solution of the present invention will be further described in detail with reference to the following specific examples.
In the following examples, unless otherwise specified, the experimental methods used were all conventional methods, and materials, reagents and the like used were all available from biological or chemical reagents companies.
The formulations of the media required in the following examples are as follows:
1. enriching and screening a liquid culture medium by high-salinity ammonia nitrogen degrading bacteria: glucose 5g, NH4Cl 1g，K2HPO4 1.0g，MgSO4·7H2O 0.1g，FeSO4·7H20.1g of O, 30g of NaCl, 7.0-8.0 of pH and 1L of distilled water.
2. Separating and purifying the solid culture medium by the high-salinity nitrate nitrogen degrading bacteria: grapeGlucose 5g, NH4Cl 1g，K2HPO4 1.0g，MgSO4·7H2O 0.1g，FeSO4·7H20.1g of O, 30g of NaCl, 15g of agar powder, 7.0-8.0 of pH and 1L of distilled water.
3. Modified Nutrient Broth (NB) medium: beef extract 5g, peptone 10g, sodium chloride 5g, NH4Cl 2g/L、FeSO4·7H2O 0.1g/L、MgSO4·7H20.1g/L of O and 1L, pH 7.0.0-7.5 of water.
4. Modified Nutrient Broth (NB) solid medium: beef extract 5g, peptone 10g, sodium chloride 5g, NH4Cl 2g/L、FeSO4·7H2O 0.1g/L、MgSO4·7H20.1g/L of O, 15g of agar powder and 1L, pH 7.0.0-7.5 of water.
5. Fermentation medium: 30g/L glucose, 5g/L peptone, 5g/L yeast powder and 5g/L, NaCl 10g/L, NH corn steep liquor dry powder4Cl 2g/L、KH2PO4 0.5g/L、FeSO4·7H2O 0.1g/L、Na2HPO4 2g/L、MgSO4·7H20.1g/L of O, the balance of water, and the pH value of the liquid culture medium is 7.0-7.5.
The above culture medium is sterilized at 121 deg.C for 20min before use, and then stored at room temperature.
Example 1: screening, separation and identification of Alcaligenes faecalis GBW-HB1905
1. Screening and purifying Alcaligenes faecalis GBW-HB1905
Taking 15mL of mixed sewage to be treated in a municipal sewage plant, adding the mixed sewage into 135mL of PBS buffer solution, oscillating for 20min to fully mix the samples, centrifuging for 1min at 1000rpm, and collecting the sample supernatant for later use. And adding 1mL of the sample supernatant into a conical flask filled with 100mL of the high-salinity ammoniacal nitrogen degrading bacterium enrichment screening liquid culture medium, and carrying out shaking culture at the constant temperature of 28 ℃ and 180rpm for 2d for enrichment for 3 times. After the cultured bacterial suspension is diluted in a gradient way, 100 mu L of the bacterial suspension is coated on a solid culture medium for enriching and screening the high-salinity ammonia nitrogen degrading bacteria and is placed in an incubator at 28 ℃ for culture. After 48h, single colonies of different morphologies were picked and streaked on modified Nutrient Broth (NB) solid medium, and isolation and purification were repeated 3 times to obtain a single colony, which was named as GBW-HB 1905.
As shown in figure 1, the bacterial colony of the bacterial strain GBW-HB1905 on a modified Nutrient Broth (NB) solid medium plate is round, milk white, 1-2mm in diameter, smooth, moist and slightly glossy in surface, flatter, slightly convex in middle, opaque, neat in edge and free of halo.
2. Identification of Alcaligenes faecalis GBW-HB1905
The DNA of the strain GBW-HB1905 is used as a template, 16S rRNA universal primers are used for amplification, sequence determination is carried out on amplified fragments, the 16S rDNA sequencing result of the obtained strain GBW-HB1905 is compared with the sequence in GenBank for analysis, and the result shows that the homology of the strain GBW-HB1905 and Alcaligenes faecalis is the highest, so that the GBW-HB1905 strain is determined to be Alcaligenes faecalis.
And (3) performing strain preservation on the screened strain GBW-HB1905, wherein the preservation unit of the Alcaligenes faecalis GBW-HB1905 is as follows: china general microbiological culture Collection center (CGMCC); address: western road No. 1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: year 2020, month 07, 15; the preservation number of the Alcaligenes faecalis is as follows: CGMCC No. 20364.
Example 2: growth determination and physiological and biochemical characteristics of Alcaligenes faecalis GBW-HB1905
1. Growth assay for Alcaligenes faecalis GBW-HB1905
Inoculating the slant-cultured Alcaligenes faecalis GBW-HB1905 into an improved NB culture medium, culturing for 22h in a constant-temperature shaking table with the pH of 7.0-7.5 and the temperature of 28 ℃ to prepare GBW-HB1905 bacterial liquid, sampling once every 1.0h, measuring the light absorption value under OD600nm, and drawing a growth curve chart. As shown in FIG. 2, Alcaligenes faecalis GBW-HB1905 is in growth retardation phase in the first 4h of culture, then enters logarithmic growth phase, and enters the end stage of logarithmic growth when being cultured for 14-16h, and the number of bacteria reaches 6.0 × 109CFU/mL, 16-20h is a growth stabilization phase, followed by a decline phase, completing the entire growth cycle.
2. Physiological and biochemical characteristics of Alcaligenes faecalis GBW-HB1905
After the prepared GBW-HB1905 bacterial liquid is further cultured in an NB culture medium, the detection and analysis are carried out on the Alcaligenes faecalis GBW-HB1905 according to a strain physiological and biochemical detection method in Bergey' Manual of identification of bacteria. The results are shown in Table 1. In addition, the Alcaligenes faecalis GBW-HB1905 can normally grow at the temperature of 15-40 ℃, and the optimal growth temperature is 25-32 ℃; can grow in the pH value range of 6-9, and the optimum growth pH value is 7.5-8.5; can normally grow in the range that the dissolved oxygen content is less than 0.5mg/L or more than 2mg/L, so the alcaligenes faecalis GBW-HB1905 belongs to facultative anaerobe; in addition, the strain has the capability of producing urease, beta-glucosidase and decomposing glycerol, and can also degrade power-nitrate, simon citrate and semisolid agar. In addition, the formula of a suitable nutrient medium for fermenting and culturing the Alcaligenes faecalis GBW-HB1905 is as follows: 30g/L glucose, 5g/L peptone, 5g/L yeast powder and 5g/L, NaCl 10g/L, NH corn steep liquor dry powder4Cl 2g/L、KH2PO40.5g/L、FeSO4·7H2O 0.1g/L、Na2HPO4 2g/L、MgSO4·7H20.1g/L of O, the balance of water, and the pH value of the culture medium is 7.5-8.5.
TABLE 1 physiological and biochemical characteristics of Alcaligenes faecalis GBW-HB1905
Figure BDA0002840482680000051
Figure BDA0002840482680000061
Note: < + > represents positive, and < - > represents negative.
Example 3: salinity tolerance test of Alcaligenes faecalis GBW-HB1905
In order to test the survival performance of the Alcaligenes faecalis GBW-HB1905 under high salinity conditions, the growth of the Alcaligenes faecalis GBW-HB1905 under different salinity conditions was analyzed and detected. Adjusting the amount of sodium chloride in the NB culture medium to prepare culture media with different salt concentrations, inoculating the slant-cultured Alcaligenes faecalis GBW-HB1905 into the NB culture media with different salt concentrations, culturing for 18h in a constant-temperature shaking table with the pH value of 7.0-7.5 and the temperature of 28 ℃ to obtain a culture solution of the Alcaligenes faecalis GBW-HB1905, and detecting the growth conditions of the Alcaligenes faecalis in the culture media with different salt concentrations. As shown in Table 2, GBW-HB1905 has good salt-resistant growth characteristics, and can grow well within the salinity range of 15-35 per mill.
TABLE 2 growth of Alcaligenes faecalis GBW-HB1905 in different salt concentrations
Figure BDA0002840482680000062
Example 4: debugging application of Alcaligenes faecalis GBW-HB1905 in slaughter wastewater treatment system of certain food factory
1. Preparation of Alcaligenes faecalis GBW-HB1905 microbial inoculum
(1) Inoculating the slant-cultured Alcaligenes faecalis GBW-HB1905 into an improved NB culture medium, and culturing for 22h in a constant-temperature shaking table with the pH of 7.5-8.5 and the temperature of 28 ℃ to obtain an Alcaligenes faecalis GBW-HB1905 seed solution; inoculating the Alcaligenes faecalis GBW-HB1905 seed solution into a fermentation medium according to the volume ratio of 10%, adjusting the tank pressure to 0.1MPa, the temperature to 28-30 ℃, the dissolved oxygen to be not less than 25%, the stirring speed to 180rpm, and fermenting for 20h to obtain the Alcaligenes faecalis GBW-HB1905 zymocyte solution;
(2) mixing the bacillus foecalis alkaligenes GBW-HB1905 zymocyte liquid and a carrier according to the volume-mass ratio of 1: 2-4, and carrying out vacuum freeze drying at-40 to-50 ℃ to obtain dry bacterium powder;
(3) adding a carrier with the mass ratio of 1: 50-100 into the dry bacterial powder, and uniformly mixing to obtain the alcaligenes faecalis microbial inoculum; the bacterial content of the microbial inoculum is 5.0 multiplied by 108CFU/mL。
Wherein the carrier is calcium carbonate, talcum powder, diatomite or a mixture of the calcium carbonate, the talcum powder and the diatomite.
2. The alcaligenes faecalis GBW-HB1905 microbial inoculum is combined with an actual engineering case for application verification, and the system adjustment of the slaughter wastewater of a certain food factory in the Weifang city is aimed atThe basic process flow of the wastewater treatment system comprises the following steps: sump well + grid + equalizing basin + A2An O + sedimentation tank and a BAF tank; at present, the wastewater treatment project is started to operate, but the treatment system always has the problem that the COD, ammonia nitrogen and total nitrogen of effluent water do not reach the standard, the COD concentration of the effluent water is 90-110mg/L, the ammonia nitrogen concentration is 10-12mg/L, and the total nitrogen is 18-22mg/L, which are all higher than the standard of effluent water level A (level A standard: COD is less than or equal to 50mg/L, ammonia nitrogen is less than or equal to 5mg/L, and the total nitrogen is less than or equal to 15 mg/L).
In the test debugging process, a dichromate method (HJ 828 + 2017) is adopted to detect the COD concentration in the wastewater, a Nashin reagent spectrophotometry method (HJ-535 + 2009) is adopted to detect the ammonia nitrogen concentration in the wastewater, and an alkaline potassium persulfate digestion ultraviolet spectrophotometry method (GB-11894-89) is adopted to detect the total nitrogen concentration in the wastewater. Before test debugging begins, maintaining and detecting various indexes operated in a wastewater treatment system, ensuring that the dissolved oxygen in water in an aerobic section is more than or equal to 2mg/L, controlling the pH value to be between 7.0 and 8.0, and controlling the addition concentration of the bacillus foecalis alkaligenes GBW-HB1905 microbial inoculum in the aerobic section to be 200 ppm; the dissolved oxygen in the water of the anoxic section is less than or equal to 0.5mg/L, the pH value is controlled to be between 7.0 and 8.0, and the adding concentration of the alcaligenes faecalis GBW-HB1905 microbial inoculum in the anoxic section is 100 ppm. After the microbial inoculum is added, internal circulation debugging is carried out for 24-48h by matching with a field process, and then the normal running state of the system is gradually recovered; and (4) carrying out tracking detection on COD, ammonia nitrogen and total nitrogen concentration at the water outlet end of the sedimentation tank at a fixed time every day, wherein the whole test debugging period is 7 days. The change results of COD, ammonia nitrogen and total nitrogen in the wastewater in the whole debugging period are respectively shown in figure 3, figure 4 and figure 5, until the 5 th day of debugging, the COD, ammonia nitrogen and total nitrogen concentration of the effluent of the system are respectively reduced to 27mg/L, 1.8mg/L and 6.5mg/L and are all within the requirements of the first-level A standard, and then the COD, ammonia nitrogen and total nitrogen concentration in the system are continuously reduced and are continuously and stably within the range required by the effluent standard. The method shows that the alcaligenes faecalis GBW-HB1905 microbial inoculum can be used as a microbial agent to be developed into products for removing COD, ammonia nitrogen and total nitrogen in slaughter wastewater, can be used for further improving the treatment efficiency of COD, ammonia nitrogen and total nitrogen in the wastewater, improves the operation stability of a wastewater treatment system, ensures that effluent indexes reach the standard stably, and has important application value and significance.
The production process of the Alcaligenes faecalis GBW-HB1905 microbial inoculum is mature and stable, the use method is simple and convenient in practical application, the Alcaligenes faecalis is directly added into a corresponding system pool body according to the suggested addition amount, the effect is obvious, and the method has good industrialization prospect and value.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.

Claims (10)

1. A microbial agent containing alcaligenes faecalis is characterized by comprising bacteria with the content not less than 5.0 x 108CFU/g Alcaligenes faecalis powder and a carrier.
2. The microbial agent according to claim 1, wherein the mass ratio of the alcaligenes faecalis powder to the carrier is 1: 50-100.
3. The microbial agent according to claim 1, wherein the powder of Alcaligenes faecalis is produced from Alcaligenes faecalis GBW-HB1905 with the collection number CGMCC No. 20364.
4. The microbial agent according to claim 3, wherein the Alcaligenes faecalis powder is prepared by the following steps:
(1) inoculating the Alcaligenes faecalis GBW-HB1905 into an improved NB culture medium for culture to obtain an Alcaligenes faecalis GBW-HB1905 seed solution;
(2) inoculating 8-10% of the Alcaligenes faecalis GBW-HB1905 seed solution into a fermentation medium according to the volume ratio, adjusting the tank pressure to 0.1-0.2 MPa, the temperature to 28-30 ℃, the dissolved oxygen to be not less than 25%, stirring at 160-180 rpm, and fermenting for 16-20h to obtain an Alcaligenes faecalis GBW-HB1905 zymocyte solution;
(3) mixing the bacillus alcaligenes faecalis GBW-HB1905 zymocyte liquid and a carrier according to the volume-mass ratio of 1: 2-4, and carrying out vacuum freeze drying at the temperature of-40 to-50 ℃ to obtain bacillus alcaligenes faecalis GBW-HB1905 bacterial powder.
5. The microbial agent according to claim 3, wherein the Alcaligenes faecalis GBW-HB1905 has a broad salt tolerance, and the growth salinity is 15-35 ‰; the optimal growth temperature is 25-32 ℃, and the optimal pH value is 7.5-8.5.
6. The microbial agent according to claim 1, wherein the carrier is at least one of calcium carbonate, talc and diatomaceous earth.
7. Use of the microorganism bacterium agent containing Alcaligenes faecalis according to any one of claims 1 to 6 for the preparation of a preparation for degrading contaminants in slaughter wastewater.
8. The use according to claim 7, wherein the microbial agent is used by: the microbial agent is directly added into an anoxic tank and an aerobic tank, the dissolved oxygen in the water of the aerobic tank is kept to be more than or equal to 2mg/L, the dissolved oxygen in the water of the anoxic tank is kept to be less than or equal to 0.5mg/L, and the pH values of the anoxic tank and the aerobic tank are controlled to be 7.0-8.0.
9. The use according to claim 8, wherein the microbial inoculum is added in the anoxic tank and the aerobic tank of the slaughter wastewater treatment system at concentrations of 80-100ppm and 180-200ppm, respectively.
10. The use according to claim 7, wherein the contaminants comprise COD, ammonia nitrogen and total nitrogen.
CN202011490779.1A 2020-12-17 2020-12-17 Microbial agent containing alcaligenes faecalis and application thereof Pending CN112592853A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103289939A (en) * 2013-06-19 2013-09-11 重庆大学 Alcaligenes faecalis and application thereof
CN108676752A (en) * 2018-05-31 2018-10-19 中国水产科学研究院珠江水产研究所 Bacillus foecalis alkaligenes Y311 screening techniques and application
CN111117912A (en) * 2019-12-28 2020-05-08 北京翰祺环境技术有限公司 Salt-tolerant denitrifying bacteria strain, salt-tolerant denitrifying bacteria liquid, screening method and application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103289939A (en) * 2013-06-19 2013-09-11 重庆大学 Alcaligenes faecalis and application thereof
CN108676752A (en) * 2018-05-31 2018-10-19 中国水产科学研究院珠江水产研究所 Bacillus foecalis alkaligenes Y311 screening techniques and application
CN111117912A (en) * 2019-12-28 2020-05-08 北京翰祺环境技术有限公司 Salt-tolerant denitrifying bacteria strain, salt-tolerant denitrifying bacteria liquid, screening method and application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
席亚萍: "异养硝化菌的脱氮活性及功能基因的研究", 《中国优秀硕士学位论文全文数据库（工程科技I辑）》 *
陈均利 等: "同步硝化反硝化菌（Alcaligenes faecalis WT14）养殖污水脱氮效果研究", 《农业环境科学学报》 *

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