CN106635858A - Paracoccus sp. and culture application thereof - Google Patents
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- CN106635858A CN106635858A CN201510737219.4A CN201510737219A CN106635858A CN 106635858 A CN106635858 A CN 106635858A CN 201510737219 A CN201510737219 A CN 201510737219A CN 106635858 A CN106635858 A CN 106635858A
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Abstract
The invention relates to paracoccus sp. and a culture application thereof. The strain is paracoccus sp. FSTB-2, collected at 'China General Microbiological Culture Collection Center' on June 1, 2015, and the preservation number is CGMCC No. 10938. The strain can be applied to efficiently removing COD (chemical oxygen demand) in salty wastewater with salt content of 1.0wt%-8.0wt%, and can grow at the high temperature of 40 DEG C. The invention further provides COD removing salt-tolerant bacteria which comprise the paracoccus sp. FSTB-2. The COD in the high-salt wastewater can be rapidly degraded by the paracoccus sp., the paracoccus sp. is high in salt resistance and good in treatment effect, combining strains are omitted, and the production cost of the salt-tolerant bacteria is greatly reduced.
Description
Technical field
The invention belongs to field of environment microorganism, and in particular to a kind of de- COD pair coccuses of salt tolerant and its culture application, the bacterial strain is particularly well-suited to the efficient removal of COD in high slat-containing wastewater.
Background technology
Generally contain higher COD from the high slat-containing wastewater that the salt content of the industries such as oil, chemical industry, medicine and chemical fertilizer is higher than 1%, Pollutants in Wastewater is constantly discharged in environment can cause serious pollution problem.The shortage problem of freshwater resources is increasingly serious, and the process and its recycling of waste water are more subject to people's attention.
Physically or chemically method processes the COD in high slat-containing wastewater, high cost and is likely to result in secondary pollution.Traditional biological method has great advantage when Low-salinity waste water is processed, but in high salt, high soda acid, high temperature or low temperature inverse ring border, the degradation capability of microorganism will be suppressed.Facultative Halophiles technology is based on the problem of engineering field and is badly in need of and produces, and by scientific method the advantage Facultative Halophiles of process high slat-containing wastewater are tamed out, and the bacteroid can be grown with its unique eucaryotic cell structure and material composition in the environment of higher salinity.Facultative Halophiles process high slat-containing wastewater and have the unrivaled advantage of conventional activated sludge process, and than physical-chemical process low cost, can produce more obvious economic benefit and social benefit, can provide technical support for related wastewater treatment qualified discharge.
Paracoccus（Paracoccus sp.）Can be used for the degraded removing of hardly degraded organic substance, COD, ammonia nitrogen and total nitrogen in field of waste water treatment.Adopting paracoccus（Paracoccus sp.）Process ammonia nitrogen and total nitrogen technical field in waste water：CN201310061522.8 is related to one kind and bites ammonia pair coccus（Paracoccus aminovorans）LH-N40, preserving number is CGMCC No.6971, the bacterial strain can not only effectively remove ammonia nitrogen and total nitrogen in water body in same reactor, simultaneously there is tolerance or degradation capability to noxious materials such as the phenols in waste water, amine, heterocyclic, cyanogen class, multiring aromatic hydrocarbons, it is particularly well-suited to nitrogenous wastewater from chemical industry, resistance to environmental poisonous substance impact.CN201410204027.2 is related to one plant of Bangladesh pair coccus with heterotrophic nitrification and aerobic denitrification function（Paracoccus bengalensis）N74-1, preserving number is CGMCC No.9148, and the bacterial strain has heterotrophic nitrification and aerobic denitrification function, can simultaneously remove ammoniacal nitrogen and nitrite nitrogen in water body, has nitrification and denitrification dual-use function concurrently.CN201010536203.4 is related to one plant of energy to be carried out aerobic denitrification, the total nitrogen efficient removal bacterial strain Paracoccus denitrificans of heterotrophic nitrification-aerobic denitrification also can be carried out using ammonia nitrogen using nitrate nitrogen（Paracoccu sdenitrificans）DN-3, preserving number is CGMCC No.3658.CN201210144543.1 is related to one plant of Paracoccus denitrificans ZGL1 with heterotrophic denitrification, autotrophic denitrification and iron restoring function, and preserving number is CCTCC M2012158.CN201310002744.2 is related to one plant of Paracoccus denitrificans with heterotrophic nitrification function（Paracoccus denitrificans）FJAT-14899, preserving number is CGMCC No.6388.
Adopting paracoccus（Paracoccus sp.）Process hardly degraded organic substance technical field in waste water：CN201310016934.X is related to one plant of Ye Shi pair coccus with fast degradation formaldehyde function（Paracoccus yeei）Scuhtp-FD3, preserving number is CCTCC NO.M2012430.CN200610081490.8 is related to one plant of Paracoccus denitrificans to organic pollutions such as pyridine, benzene, dimethylbenzene, quinoline, cyanides with stronger degradation capability（Paracoccus denitrificans）W12, preserving number is CGMCCNo.1673.CN200810022333.9 be related to a kind of PAHs in environment have preferable degradation capability bite ammonia pair coccus（Paracoccus aminovorans）HPD-2, preserving number is CGMCC No.2568.CN201310083135.4 is related to one plant of secondary coccus PQ-01 with efficient degradation piperazine performance.CN201310200737.3 is related to one plant of paracoccus MXX-04 that can be used for Brominal efficient degradation.CN201010239621.7 is related to paracoccus（Paracoccus sp.）D17 and paracoccus（Paracoccus sp.）Two kinds of D24 has the bacterial strain of low temperature resistant oil degradation function.CN200710303975.1 is related to the secondary coccus of one plant of pyridine that can effectively degrade（Paracoccus sp.）BW001, preserving number is CGMCC No.2225.CN200910027112.5 is related to a kind of secondary coccus of the Buprofezin agricultural chemicals that can effectively degrade（Paracoccus sp.）.
In high slat-containing wastewater process field, salt content is a key factor for affecting microbiological treatment waste water efficiency.Believe glad etc.（Biological reinforcing technology processes highly salt containing organic waste water [J]. water technology, 2008 (8)：66-70）Highly salt containing organic waste water is processed using biological reinforcing technology, significantly improved using the dehydrogenase activity of the biological reinforced saponin waste water biological treatment system activated sludge of Facultative Halophiles, when system tolerance chlorine ion concentration is up to 2.8%, the clearance of saponin waste water COD is 84.41%.CN201210130657.0, CN201210130644.3, CN201010536065.X and CN201210130658.5 are related to using marsh cock Salmonella（Kocuri apalustris）FSDN-A, Staphylococcus cohnis（Staphylococcus cohnii）FSDN-C, arthrobacterium（Arthrobacter creatinolyticus）FDN-1, Shui Shi Flavobacterium（Flavobacterium mizutaii）FDN-2, Paracoccus denitrificans（Paracoccus denitrificans）DN-3 and Methylobacterium（Methylobacterium phyllosphaerae）The method that SDN-3 is carried out a biological disposal upon to the ammonia nitrogen in brine waste and catalytic cracking catalyst waste water, total nitrogen and COD.Single bacterial strain described in the method has salt resistant character, needs to cultivate various bacterial strains and is compounded, and preparation process is complicated, and production cost is higher.
At present it is known that the secondary coccus that screening is obtained has not thering is salt resistant character or salt resistant character not, the process of high slat-containing wastewater is not suitable for.
The content of the invention
It is an object of the invention to provide a kind of de- COD pair coccuses of salt tolerant and its culture application.The secondary coccus that the present invention is provided can remove the COD in high slat-containing wastewater with fast degradation, and salt resistance ability is strong, and high treating effect, it is not necessary to compound microbial inoculum significantly reduces the production cost of salt tolerant microbial inoculum.
The secondary coccus FSTB-2 that the present invention is provided, its Classification And Nomenclature is secondary coccus（Paracoccus sp.）, it is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, deposit number is CGMCC No.10938.
The secondary coccus FSTB-2 that the present invention is provided, main morphological features are：Colony colour is ecru, and it is spherical that bacterial strain is individual.Physiological and biochemical property shows as：Gram's staining is feminine gender, and oxidase positive, catalase is negative, and decomposable asymmetric choice net utilizes several kinds of carbon source, with nitrate reduction activity.
The secondary coccus FSTB-2 that the present invention is provided is resistant to one or more in lincomycin, Rifamycin Sodium, acidum nalidixicum, guanidine hydrochloride etc..
The 16SrRNA gene orders of the secondary coccus FSTB-2 that the present invention is provided are shown in sequence table.
The secondary coccus FSTB-2 that the present invention is provided removes the application in COD in brine waste.The bacterial strain can be applicable to the efficient removal of COD in the brine waste of salt content 1.0wt%-8.0wt%, and can grow under 40 DEG C of high temperature.
In the present invention, the brine waste can be the strong brine that the waste water of the generations such as chemical industry synthesis waste water, coal chemical industrial waste water, or oil refining process, Coal Chemical Industry technique and chemical industry synthesis technique through pre-processing is produced through counter-infiltration minimizing unit.The salt content of the brine waste is 0.5wt%-8.0wt%, preferred 1.0wt%-5.0wt%, COD（Cr methods, similarly hereinafter）Content is 200-20000mg/L.
The cultural method of the secondary coccus FSTB-2 that the present invention is provided, including the three phases such as bacterial strain activation, liquid seeds liquid culture, aeration culture, concretely comprise the following steps：
（1）Bacterial strain is activated：Secondary coccus FSTB-2 is inoculated in the inclined-plane of FSTB solid mediums or flat board, 25-40 DEG C of culture 24-48 hour, in being then stored in 4 DEG C of refrigerators.
（2）Liquid seeds liquid culture：Prepare FSTB fluid nutrient mediums, in being sub-packed in triangular flask, sterilize and be cooled to after room temperature, the inoculation activated in picking inclined-plane or flat board under gnotobasis in triangular flask, 25-40 DEG C of culture 24-72h.The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 6.0-8.5, preferably 6.5-8.0；The FSTB solid mediums are addition 20g/L agar in liquid medium within.
（3）Aeration culture：FSTB-2 fluid nutrient mediums are added in the closed reactor for be provided with aerator, ratio inoculation liquid seed liquor according to reactor volume than 0.5%-25%, pH value is controlled in 6.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, also a small amount of carbon source can be added, nitrogen source and microelement substance, culture 24-48 hours are 1 cultivation cycle, discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards, thus the dense bacterium solution product containing higher concentration pair coccus is obtained.
In the present invention, after culture terminates, the dense bacterium solution product that collection culture is finished, during the flat board containing FSTB solid mediums is applied to after dilution, the bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-2.Under closed culture environment, secondary coccus is pure bacterial strain, and percent similarity reaches more than 95%.
The present invention also provides a kind of salt tolerant microbial inoculum of de- COD, and the microbial inoculum includes secondary coccus（Paracoccus sp.）FSTB-2.Described salt tolerant microbial inoculum can be only with salt tolerant microbial inoculum obtained in secondary coccus FSTB-2 bacterium solutions, can also be with other salt tolerant microbial inoculum compoundings, such as can be combined with the existing microbial bacterial agent with denitrogenation, dephosphorization and degraded hardly degraded organic substance, be realized more preferable water treatment effect.
The salt tolerant pair coccus FSTB-2 that the present invention is provided is one plant of bacterial strain that in Halite water body there is preferable COD to remove performance, salt resistance ability is strong, and high treating effect is used directly for the process of brine waste, during existing brine waste processing system can also be added to, water treatment effect is improved.The cultural method of the secondary coccus FSTB-2 that the present invention is provided, can directly utilize sewage treatment plant's brine waste（A small amount of natural medium and chemical synthesis culture medium are added according to condition of water quality）Dominant strain culture enrichment enrichment work is carried out, the growth characteristics and resistance characteristics of salt tolerant microbial inoculum are greatly maintained, and reduces the toxigenic capacity of salt tolerant microbial inoculum.
Biomaterial preservation explanation
The secondary coccus that the present invention is provided（Paracoccus sp.）FSTB-2 bacterial strains, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）；Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica；Deposit number：CGMCC No.10938；Preservation date：On June 1st, 2015.
Specific embodiment
The secondary coccus of the present invention（Paracoccus sp.）FSTB-2 is in June, 2014 to be obtained from taking from Hunan Yueyang petroleum chemical enterprise for separation screening in the activated sludge for processing epoxychloropropane production waste water, and its physiological and biochemical test the results are shown in Table 1.
The physiological and biochemical test result of the secondary coccus FSTB-2 of table 1
Jing " China Committee for Culture Collection of Microorganisms's common micro-organisms center " identifies that the bacterial strain belongs to secondary meningitidis strains（Paracoccus sp.）, it is named as FSTB-2.
Embodiment 2 is screened and isolated and purified
Domestication culture 15 days is carried out to the activated sludge for taking from Hunan Yueyang petroleum chemical enterprise for processing epoxychloropropane production waste water using the method for gradually stepping up salinity in nutrient solution.
The good activated sludge of above-mentioned domestication is taken, initial gross separation is carried out in enriched medium using the method for dilution spread, thus obtain 15 bacterium colonies that growth conditions are good and proterties is different.The main component that the enriched medium for being adopted contains for：COD is 1000mg/L, and calcium ion concentration is 1000mg/L, and total salt quantity is 10000mg/L, and agar content is for 2.0% in the solid medium for being adopted.
Then the bacterium colony for obtaining is transferred using the method for plate streaking, line is transferred in screening and culturing medium, is further separated and is screened.The main component that the screening and culturing medium for adopting contains for：COD is 1000mg/L, and calcium ion concentration is 4000mg/L, and total salt quantity is 25000mg/L, and agar content is for 2.0% in the solid medium for being adopted.
10 plants of pure bacterial strains are obtained by aforesaid operations, wherein one plant is secondary coccus（Paracoccus sp.）FSTB-2.
The investigation of the secondary coccus FSTB-2 salt resistance abilities of embodiment 3
The cultural method of secondary coccus FSTB-2 of the present invention includes：Bacterial strain activation, liquid seeds liquid culture, aeration culture, detailed process is as follows：
（1）Bacterial strain is activated：By secondary coccus（Paracoccus sp.）FSTB-2 is inoculated in the flat board containing FSTB solid mediums, is cultivated 48 hours under 35 DEG C of environment, is then stored in stand-by in 4 DEG C of refrigerators.The FSTB solid mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, agar 20g/L, pH value 7.8.
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, in being sub-packed in triangular flask, is sterilized and is cooled to after room temperature, cultivated 48 hours under the conditions of 35 DEG C in triangular flask with the inoculation after activation in oese picking flat board under gnotobasis.The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value 7.8.
（3）Aeration culture：Cultivated using closed reactor，It is complete that institute is required to sterilizing using reactor and various utensils，Air inlet and exhaust position need to install bacteriological filter apparatus，Nutrient solution、Acid-base modifier and trace element solution are required to be added according to sterile working code after sterilizing，Fermentation tank need to be equipped with aerator，And can carry out into water、Acid adjustment、Alkali tune、Feed supplement and draining discharge are operated，The FSTB fluid nutrient mediums being put in the reactor after sterilizing，Ratio according to percent by volume 10% is inoculated with liquid seed liquor，After opening incubation，Adopt soda acid automatic control system by medium pH value scope control in 6.0-8.5 in incubation，Aeration culture carries out afterwards periodic feed supplement and discharge operation for 72 hours，Discharge is the nutrient solution of the 25% of reactor volume，Feed supplement is the FSTB fluid nutrient mediums of reactor volume 25%，Also simultaneously a small amount of carbon source can be added by aseptic charging device、Nitrogen source and microelement substance，Culture 24 hours is 1 cultivation cycle，Discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards，Thus the dense bacterium solution product containing pure secondary coccus is obtained.
The dense bacterium solution that above-mentioned culture is finished is gathered, during the flat board containing FSTB solid mediums is applied to after dilution, the bacterium colony to growing is counted and counted the percentage of bacterium colony similar to FSTB-2, and percent similarity reaches more than 95%, that is, assert that the secondary coccus is pure bacterial strain.
The secondary coccus FSTB-2 obtained using above-mentioned culture processes brine waste.By taking the epichlorohydrin production process brine waste through pretreatment as an example, the water quality of handled brine waste is the present embodiment：COD concentration is 800-2000mg/L, and salt content is 1wt%-8wt%, treatment temperature 30-40 DEG C.Treatment effect is as shown in table 2.
The secondary coccus FSTB-2 of table 2 processes the effect of brine waste
As shown in Table 2, the salt content that secondary coccus FSTB-2 is resistant to is 1wt%-8wt%, can be grown under 40 DEG C of high temperature, can be with the COD in efficient removal brine waste.
Secondary coccus FSTB-2 of the present invention is very good to the adaptability and treatment effect of actual brine waste, with stronger COD degradation function, individually brine waste can be processed using the pure bacterial strain, also can individually amplify be added to existing process flow process as external source functional microorganism after culture, targetedly regulation is optimized to actual process situation, solves the problems, such as active sludge treatment effect difference and easily degeneration under hypersaline environment.High slat-containing wastewater is processed than physical-chemical process low cost, direct application can produce more obvious economic benefit and social benefit, can provide technical support for high saliferous correlation wastewater treatment qualified discharge using Facultative Halophiles.
SEQUENCE
LISTING
<110>
Sinopec Group
Dalian Petroleum Chemical Engineering Institute of Sinopec Group
<120>
A kind of secondary coccus and its culture application
<160> 1
<170> PatentIn version 3.5
<210>
1
<211>
1295
<212>
DNA
<213> Paracoccus sp.
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agccctgggaaactgggagtaataccgcatgagccctacgggggaaagatttatcggcaa
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aggatcggcccgcgttggattaggtagttggtggggtaatggcctaccaagccgacgatc
180
catagctggtttgagaggatgatcagcccacactgggactgagacacggcccagactcct
240
acgggaggcagcagtggggaatcttagacaatgggggcaaccctgatctagccatgccgc
300
gtgagtgatgaaggccttagggttgtaaagctctttcagctgggaagataatgacggtac
360
cagcagaagaagccccggctaactccgtgccagcagccgcggtaatacggagggggctag
420
cgttgttcggaattactgggcgtaaagcgcacgtaggcggaccagaaagttgggggtgaa
480
atcccggggctcaacctcggaactgccttcaaaactattggtctggagttcgagagaggt
540
gagtggaattccgagtgtagaggtgaaattcgtagatattcggaggaacaccagtggcga
600
aggcggctcactggctcgatactgacgctgaggtgcgaaagcgtggggagcaaacaggat
660
tagataccctggtagtccacgccgtaaacgatgaatgccagtcgtcgggcagcatgctgt
720
tcggtgacacacctaacggattaagcattccgcctggggagtacggtcgcaagattaaaa
780
ctcaaaggaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgaagcaa
840
cgcgcagaaccttaccaacccttgacatcctcggatcggcctggagacaggtcttccact
900
tcggtggccgagtgacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttcgg
960
ttaagtccggcaacgagcgcaacccacactttcagttgccatcatttggttgggcactct
1020
ggaagaactgccgatgataagtcggaggaaggtgtggatgacgtcaagtcctcatggccc
1080
ttacgggttgggctacacacgtgctacaatggtggtgacagtgggttaatccccaaaagc
1140
catctcagttcggattggggtctgcaactcgaccccatgaagttggaatcgctagtaatc
1200
gcggaacagcatgccgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacc
1260
atgggagttgggtctacccgacggccgtgcgctaa
1295
Claims (10)
1. a kind of secondary coccus FSTB-2, its Classification And Nomenclature is secondary coccus（Paracoccus sp.）, it is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, deposit number is CGMCCNo.10938.
2. bacterial strain according to claim 1, it is characterised in that：The morphological feature of the bacterial strain is：Colony colour is ecru, and it is spherical that bacterial strain is individual.
3. bacterial strain according to claim 1, it is characterised in that：The physiological and biochemical property of the bacterial strain is：Gram's staining is feminine gender, and oxidase positive, catalase is negative, and decomposable asymmetric choice net utilizes several kinds of carbon source, with nitrate reduction activity.
4. bacterial strain according to claim 1, it is characterised in that：The bacterial strain is resistant to one or more in lincomycin, Rifamycin Sodium, acidum nalidixicum, guanidine hydrochloride etc..
5. pair coccus FSTB-2 described in claim 1 removes the application in COD in brine waste.
6. application according to claim 5, it is characterised in that：The bacterial strain is applied to the efficient removal of COD in the brine waste of salt content 1.0wt%-8.0wt%, and can grow under 40 DEG C of high temperature.
7. application according to claim 5, it is characterised in that：The brine waste is chemical industry synthesis waste water, the coal chemical industrial waste water through pre-processing, or the strong brine that the waste water of oil refining process, Coal Chemical Industry technique and chemical industry synthesis technique generation is produced through counter-infiltration minimizing unit, salt content is 0.5wt%-8.0wt%, and COD contents are 200-20000mg/L.
8. the cultural method of secondary coccus FSTB-2 according to claim 1, it is characterised in that including three phases such as bacterial strain activation, liquid seeds liquid culture, aeration cultures, concretely comprise the following steps：
（1）Bacterial strain is activated：Secondary coccus FSTB-2 is inoculated in the inclined-plane of FSTB solid mediums or flat board, 25-40 DEG C of culture 24-48 hour, in being then stored in 4 DEG C of refrigerators；
（2）Liquid seeds liquid culture：Prepare FSTB fluid nutrient mediums, in being sub-packed in triangular flask, sterilize and be cooled to after room temperature, the inoculation activated in picking inclined-plane or flat board under gnotobasis in triangular flask, 25-40 DEG C of culture 24-72h；
（3）Aeration culture：FSTB-2 fluid nutrient mediums are added in the closed reactor for be provided with aerator, ratio inoculation liquid seed liquor according to reactor volume than 0.5%-25%, pH value is controlled in 6.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, culture 24-48 hours are 1 cultivation cycle, discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards, obtain the dense bacterium solution product containing higher concentration pair coccus.
9. method according to claim 8, it is characterised in that：The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 6.0-8.5；FSTB solid mediums are addition 20g/L agar in liquid medium within.
10. a kind of salt tolerant microbial inoculum of de- COD, it is characterised in that：Microbial inoculum includes secondary coccus（Paracoccus sp.）FSTB-2.
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| CN110452858A (en) * | 2019-09-12 | 2019-11-15 | 中国科学院微生物研究所 | One Paracoccus B54 and its application |
| CN110656071A (en) * | 2019-11-11 | 2020-01-07 | 黄河三角洲京博化工研究院有限公司 | Paracoccus huilkii for efficiently degrading DMF (dimethyl formamide) and application thereof |
| CN111348752A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Treatment method of high-salt degradation-resistant wastewater |
| CN112522152A (en) * | 2020-12-16 | 2021-03-19 | 青岛尚德生物技术有限公司 | Salt-tolerant paracoccus denitrificans GBW-HB1904 and application thereof |
| CN112723558A (en) * | 2020-12-16 | 2021-04-30 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate |
| CN113307447A (en) * | 2021-05-24 | 2021-08-27 | 东华大学 | Zero-valent iron mixed culture denitrification coupling modified charcoal two-stage PRB underground water denitrification method and reactor |
| CN114292789A (en) * | 2021-12-31 | 2022-04-08 | 青岛蔚蓝赛德生物科技有限公司 | Paracoccus with denitrification and dephosphorization functions and application thereof |
| CN114410545A (en) * | 2022-02-23 | 2022-04-29 | 中国科学院微生物研究所 | Paracoccus strain GN-9 and application thereof |
| CN114456974A (en) * | 2022-01-14 | 2022-05-10 | 浙江台州秀川科技有限公司 | Paracoccus thiooxidans capable of efficiently degrading DMF (dimethyl formamide) and application thereof in treatment of waste water containing DMF |
| CN114940957A (en) * | 2022-05-11 | 2022-08-26 | 江苏科技大学 | Paracoccus pantotrophus with facultative denitrification synchronous nitrogen and phosphorus removal performance |
| CN114958689A (en) * | 2022-06-29 | 2022-08-30 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
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| CN110656071B (en) * | 2019-11-11 | 2022-03-04 | 黄河三角洲京博化工研究院有限公司 | Paracoccus huilkii for efficiently degrading DMF (dimethyl formamide) and application thereof |
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| CN114456974A (en) * | 2022-01-14 | 2022-05-10 | 浙江台州秀川科技有限公司 | Paracoccus thiooxidans capable of efficiently degrading DMF (dimethyl formamide) and application thereof in treatment of waste water containing DMF |
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| CN114940957B (en) * | 2022-05-11 | 2024-01-30 | 江苏科技大学 | Paracoccus ubitus with facultative denitrification synchronous denitrification and dephosphorization performances |
| CN114940957A (en) * | 2022-05-11 | 2022-08-26 | 江苏科技大学 | Paracoccus pantotrophus with facultative denitrification synchronous nitrogen and phosphorus removal performance |
| CN114958689A (en) * | 2022-06-29 | 2022-08-30 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
| CN114958689B (en) * | 2022-06-29 | 2023-03-10 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
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