CN106635858B - Paracoccus and culture application thereof - Google Patents
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Abstract
The invention relates to paracoccus and culture application thereof. The strain is Paracoccus (A)Paracoccus sp.) FSTB-2, which has been deposited in the China general microbiological culture Collection center (CGMCC) at 1/6/2015 with the collection number of CGMCC No. 10938. The strain can be applied to the efficient removal of COD in saline wastewater with the salt content of 1.0-8.0 wt%, and can grow at a high temperature of 40 ℃. The invention also provides a salt-tolerant microbial inoculum for removing COD, which comprises paracoccus (A)Paracoccus sp.) FSTB-2. The paracoccus provided by the invention can rapidly degrade and remove COD (chemical oxygen demand) in high-salt-content wastewater, has strong salt resistance and good treatment effect, does not need a compound microbial inoculum, and greatly reduces the production cost of the salt-tolerant microbial inoculum.
Description
Technical Field
The invention belongs to the field of environmental microorganisms, and particularly relates to salt-tolerant paracoccus COD removal and culture application thereof.
Background
High-salt-content wastewater with salt content higher than 1% from industries such as petroleum, chemical industry, medicine and chemical fertilizer generally contains higher COD, and serious pollution problems can be caused when pollutants in the wastewater are continuously discharged into the environment. The shortage of fresh water resources is becoming more serious, and the treatment and recycling of waste water are more important.
The physical or chemical method for treating COD in the wastewater with high salt content is high in cost and can cause secondary pollution. The traditional biological method has great advantages in treating low-salinity wastewater, but the degradation capability of microorganisms is inhibited in high-salt, high-acid-base, high-temperature or low-temperature adverse environments. The halotolerant bacteria technology is generated based on the problems and urgent needs in the engineering field, the dominant halotolerant bacteria for treating the high-salinity wastewater are domesticated by a scientific method, and the thalli can grow in the environment with higher salinity by using the unique cell structure and substance composition of the thalli. The halotolerant bacteria has incomparable advantages of a common activated sludge process for treating high-salt wastewater, has lower cost than a physical and chemical process, can generate obvious economic and social benefits, and can provide technical support for the standard discharge of related wastewater treatment.
Genus Paracoccus (A)Paracoccus sp.) Can be used for degrading and removing refractory organic matters, chemical oxygen demand, ammonia nitrogen and total nitrogen in the field of wastewater treatment. In the genus Paracoccus: (Paracoccus sp.) The technical field of treating ammonia nitrogen and total nitrogen in wastewater: CN201310061522.8 relates to Paracoccus aminovoransParacoccus aminovorans) LH-N40 with the preservation number of CGMCC No.6971, the strain not only can effectively remove ammonia nitrogen and total nitrogen in water in the same reactor, but also has tolerance or degradation capability to phenols, amines, heterocycles, cyanides, polycyclic aromatic hydrocarbons and other toxic substances in wastewater, and is particularly suitable for nitrogen-containing chemical wastewater and environmental poison impact resistance. CN201410204027.2 relates to a Paracoccus menganensis (B.menganensis) with heterotrophic nitrification and aerobic denitrification functionsParacoccus bengalensis) N74-1 with the preservation number of CGMCC No.9148, the strain has heterotrophic nitrification and aerobic denitrification functions, can simultaneously remove ammonia nitrogen and nitrite nitrogen in water and has double functions of nitrification and denitrification. CN201010536203.4 relates to a bacterial strain paracoccus denitrificans capable of efficiently removing total nitrogen by using nitrate nitrogen for aerobic denitrification and using ammonia nitrogen for heterotrophic nitrification-aerobic denitrificationParacoccu sdenitrificans) DN-3 with the preservation number of CGMCC No. 3658. CN201210144543.1 relates to paracoccus denitrificans ZGL1 with heterotrophic denitrification, autotrophic denitrification and iron reduction functions, and the preservation number is CCTCC M2012158. CN201310002744.2 relates to paracoccus denitrificans (A) with heterotrophic nitrification functionParacoccus denitrificans) FJAT-14899 with the preservation number of CGMCC No. 6388.
In the genus Paracoccus: (Paracoccus sp.) The technical field of treating refractory organics in wastewater: CN201310016934.X relates to Paracoccus Yersiniae (A. Yersinia) with function of rapidly degrading formaldehydeParacoccus yeei) scuhtp-FD3 with the preservation number of CCTCC NO. M2012430. CN200610081490.8 relates to paracoccus denitrificans (P.denitrificans) with strong degradation capability on organic pollutants such as pyridine, benzene, xylene, quinoline, cyanide and the likeParacoccus denitrificans) W12 with preservation number of CGMCCNo.1673. CN200810022333.9 relates to Paracoccus aminovorans with better degradation capability on PAHs in environmentParacoccus aminovorans) HPD-2 with preservation number of CGMCCNo.2568. CN201310083135.4 relates to paracoccus PQ-01 with efficient piperazine degradation performance. CN201310200737.3 relates to a Paracoccus MXX-04 capable of being used for high-efficiency degradation of bromoxynil. CN201010239621.7 relates to the genus Paracoccus: (Paracoccus sp.) D17 and Paracoccus (Paracoccus sp.) D24 two strains with low-temperature petroleum degradation resistance. CN200710303975.1 relates to a paracoccus capable of effectively degrading pyridineParacoccus sp.) BW001 with preservation number CGMCC No. 2225. CN200910027112.5 relates to paracoccus capable of effectively degrading buprofezin pesticideParacoccus sp.）。
In the field of high-salt wastewater treatment, the salt content is a key factor influencing the wastewater treatment efficiency of microorganisms. Xinxin and the like (biological strengthening technology for treating high-salt organic wastewater [ J)]Water treatment technology, 2008 (8): 66-70) adopts a biological strengthening technology to treat the organic wastewater with high salt content, the dehydrogenase activity of activated sludge of a saponin wastewater biological treatment system biologically strengthened by halotolerant bacteria is obviously improved, and the removal rate of COD (chemical oxygen demand) of the saponin wastewater is 84.41% when the concentration of chloride ions endured by the system is up to 2.8%. CN201210130657.0, CN201210130644.3, CN201010536065.X and CN201210130658.5 relate to the use of Coccocus palustris (C.palustris)Kocuri apalustris) FSDN-A, Staphylococcus cohnii (Staphylococcus cohnii) FSDN-C, Arthrobacter: (Arthrobacter creatinolyticus) FDN-1, Flavobacterium aquatile: (Flavobacterium mizutaii) FDN-2, Paracoccus denitrificans: (Paracoccus denitrificans) DN-3 and Methylobacterium (M) ((M))Methylobacterium phyllosphaerae) SDN-3 is a biological treatment method for ammonia nitrogen, total nitrogen and chemical oxygen demand in salt-containing wastewater and catalytic cracking catalyst wastewater. The single strain does not have salt tolerance, multiple strains need to be cultured for compounding, the preparation process is complex, and the production cost is high.
At present, the paracoccus obtained by screening has the problems of no salt tolerance or poor salt tolerance and the like, and is not suitable for treating high-salt-content wastewater.
Disclosure of Invention
The invention aims to provide a salt-tolerant paracoccus COD-removing bacterium and a culture application thereof. The paracoccus provided by the invention can rapidly degrade and remove COD (chemical oxygen demand) in high-salt-content wastewater, has strong salt resistance and good treatment effect, does not need a compound microbial inoculum, and greatly reduces the production cost of the salt-tolerant microbial inoculum.
The Paracoccus FSTB-2 provided by the invention is classified and named as Paracoccus (A)Paracoccus sp.) The strain is preserved in China general microbiological culture Collection center (CGMCC) at 1/6/2015 with the preservation number of CGMCC No. 10938.
The paracoccus FSTB-2 provided by the invention has the main morphological characteristics that: the colony color is beige, and the individual strain is spherical. The physiological and biochemical characteristics are shown as follows: gram staining is negative, oxidase is positive, catalase is negative, various carbon sources can be decomposed and utilized, and nitrate reduction activity is realized.
The paracoccus FSTB-2 provided by the invention can tolerate one or more of lincomycin, rifamycin SV, nalidixic acid, guanidine hydrochloride and the like.
The 16SrRNA gene sequence of the paracoccus FSTB-2 provided by the invention is shown in a sequence table.
The invention provides application of paracoccus FSTB-2 in removing COD (chemical oxygen demand) from salt-containing wastewater. The strain can be applied to the efficient removal of COD in saline wastewater with the salt content of 1.0-8.0 wt%, and can grow at a high temperature of 40 ℃.
In the invention, the salt-containing wastewater can be pretreated chemical synthesis wastewater and coal chemical wastewater, and can also be strong brine generated by wastewater generated by an oil refining process, a coal chemical process, a chemical synthesis process and the like through a reverse osmosis reduction unit. The salt content of the salt-containing wastewater is 0.5-8.0 wt%, preferably 1.0-5.0 wt%, and the COD (Cr method, the same below) content is 200-20000 mg/L.
The culture method of paracoccus FSTB-2 provided by the invention comprises three stages of strain activation, liquid seed liquid culture, aeration culture and the like, and comprises the following specific steps:
(1) strain activation: inoculating Paracoccus FSTB-2 into slant or plate of FSTB solid culture medium, culturing at 25-40 deg.C for 24-48 hr, and storing in 4 deg.C refrigerator.
(2) Liquid seed liquid culture: preparing FSTB liquid culture medium, subpackaging in triangular flask, sterilizing, cooling to room temperature, selecting activated strain in slant or flat plate under aseptic environment, inoculating into triangular flask, and culturing at 25-40 deg.C for 24-72 h. The FSTB liquid culture medium comprises: FeSO4•7H2O 25mg/L，NH4NO3286mg/L，KCl 929mg/L，CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH 6.0-8.5, preferably 6.5-8.0; the FSTB solid culture medium is prepared by adding 20g/L agar into a liquid culture medium.
(3) Aeration culture: adding an FSTB-2 liquid culture medium into a closed reactor provided with an aeration device, inoculating a liquid seed solution according to the proportion of 0.5-25% of the volume ratio of the reactor, controlling the pH value to be 6.0-8.5, carrying out aeration culture for 48-96 hours, then carrying out periodic material feeding and discharging operation, wherein the discharging amount accounts for 5-90% of the volume of the reactor, the feeding amount accounts for 5-90% of the volume of the reactor, a small amount of carbon source, nitrogen source and trace element substances can also be supplemented, culturing for 24-48 hours to form 1 culture period, and then discharging the culture solution with the corresponding volume according to the proportion, thereby obtaining a concentrated bacterial liquid product containing higher-concentration paracoccus.
In the invention, after the culture is finished, a concentrated bacterial liquid product after the culture is finished is collected, diluted and coated on a flat plate containing an FSTB solid culture medium, and grown colonies are counted and the percentage of colonies similar to FSTB-2 is counted. In a closed culture environment, the paracoccus is a pure strain, and the similarity percentage reaches more than 95 percent.
The invention also provides a salt-tolerant microbial inoculum for removing COD, which comprises paracoccus (A)Paracoccus sp.) FSTB-2. The salt-tolerant microbial inoculum can be prepared by only adopting paracoccus FSTB-2 bacterial liquid, and can also be compounded with other salt-tolerant microbial inocula, for example, the salt-tolerant microbial inocula can be combined with the existing microbial inocula with functions of denitrification, dephosphorization and degradation of refractory organic matters, so that a better wastewater treatment effect is realized.
The halotolerant paracoccus FSTB-2 provided by the invention is a strain with better COD removal performance in a high-salinity water body, has strong salt tolerance and good treatment effect, can be directly used for treating salt-containing wastewater, and can also be added into the existing salt-containing wastewater treatment system to improve the wastewater treatment effect. The method for culturing the paracoccus FSTB-2 can directly utilize the saline wastewater of a sewage treatment plant (a small amount of natural culture medium and chemically synthesized culture medium are supplemented according to water quality conditions) to carry out dominant strain culture enrichment and thickening work, greatly maintains the growth characteristic and the tolerance characteristic of the salt-tolerant microbial inoculum, and reduces the culture cost of the salt-tolerant microbial inoculum.
Biological material preservation instructions
Paracoccus according to the present invention (Paracoccus sp.) The FSTB-2 strain is preserved in China general microbiological culture Collection center (CGMCC); address: the institute of microbiology, national academy of sciences No.3, Xilu No.1, Beijing, Chaoyang, Beijing; the preservation number is: CGMCC No. 10938; the preservation date is as follows: year 2015, 6 months and 1 day.
Detailed Description
Paracoccus of the present invention (Paracoccus sp.) FSTB-2 was obtained in 2014 by separating and screening activated sludge used for treating wastewater from epichlorohydrin production by a petrochemical enterprise in Yueyang City of Hunan province, and the results of physiological and biochemical tests thereof are shown in Table 1.
TABLE 1 results of physiological and biochemical tests of Paracoccus FSTB-2
Identified by the China general microbiological culture Collection center, the strain belongs to a paracoccus strainParacoccus sp.) Named FSTB-2.
Example 2 screening and isolation and purification
The method for gradually increasing the salt concentration in the culture solution is adopted to perform acclimation culture on activated sludge which is taken from a certain petrochemical enterprise in Yueyang City of Hunan province and used for treating wastewater generated in epichlorohydrin production for 15 days.
Taking the domesticated activated sludge, and carrying out primary separation in an enrichment culture medium by adopting a dilution coating method, thereby obtaining 15 colonies with good growth states and different properties. The adopted enrichment medium contains the following main components: COD is 1000mg/L, calcium ion concentration is 1000mg/L, total salt content is 10000mg/L, and agar content in the adopted solid culture medium is 2.0%.
And then, the obtained bacterial colonies are transferred by adopting a plate streaking method, streaking is transferred into a screening culture medium, and further separation and screening are carried out. The adopted screening culture medium comprises the following main components: COD is 1000mg/L, calcium ion concentration is 4000mg/L, total salt content is 25000mg/L, and agar content in adopted solid culture medium is 2.0%.
Obtaining 10 pure strains by the operation, wherein one strain is paracoccus (A)Paracoccus sp.）FSTB-2。
EXAMPLE 3 examination of salt tolerance of Paracoccus FSTB-2
The culture method of the paracoccus FSTB-2 comprises the following steps: the method comprises the following specific processes of strain activation, liquid seed liquid culture and aeration culture:
(1) strain activation: paracoccus (A), (B) and (C)Paracoccus sp.) FSTB-2 was inoculated into a plate containing FSTB solid medium, cultured at 35 ℃ for 48 hours, and then stored in a refrigerator at 4 ℃ until use. The FSTB solid culture medium comprises: FeSO4•7H2O 25mg/L，NH4NO3286mg/L，KCl 929mg/L，CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, agar 20g/L, and pH 7.8.
(2) Liquid seed liquid culture: preparing an FSTB liquid culture medium, subpackaging in a triangular flask, sterilizing, cooling to room temperature, selecting the activated strain in the flat plate by using an inoculating loop in an aseptic environment, inoculating into the triangular flask, and culturing for 48 hours at the temperature of 35 ℃. The FSTB liquid culture medium comprises: FeSO4•7H2O 25mg/L，NH4NO3286mg/L，KCl 929mg/L，CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, and pH 7.8.
(3) Aeration culture: the method comprises the steps of adopting a closed reactor for culture, completely sterilizing the adopted reactor and various appliances, installing a bacteria filtering device at air inlet and exhaust positions, adding a culture solution, an acid-base regulator and a trace element solution according to an aseptic operation rule after sterilizing, arranging an aeration device in a fermentation tank, performing water inlet, acid regulation, alkali regulation, material supplementing and drainage and discharge operations, putting a sterilized FSTB liquid culture medium into the reactor, inoculating a liquid seed solution according to the volume percentage of 10%, starting the culture process, controlling the pH value range of the culture solution to be 6.0-8.5 by adopting an acid-base automatic control system in the culture process, performing periodic material supplementing and discharge operations after carrying out aeration culture for 72 hours, discharging the culture solution which is 25% of the volume of the reactor, supplementing the FSTB liquid culture medium which is 25% of the volume of the reactor, and simultaneously supplementing a small amount of carbon source, adding a small amount of carbon source, and discharging, And (3) culturing nitrogen sources and trace element substances for 24 hours to form 1 culture period, and then discharging culture solution with corresponding volume according to the proportion to obtain a concentrated bacterial solution product containing pure paracoccus.
Collecting the cultured concentrated bacterial liquid, diluting, spreading on a flat plate containing an FSTB solid culture medium, counting the grown bacterial colonies, counting the percentage of the bacterial colonies similar to the FSTB-2, and determining that the paracoccus is a pure bacterial strain when the similar percentage reaches more than 95%.
And treating the saline wastewater by adopting the paracoccus FSTB-2 obtained by the culture. In this embodiment, the pretreated wastewater containing salt in the epichlorohydrin production process is taken as an example, and the water quality of the treated wastewater containing salt is as follows: the COD concentration is 800-2000mg/L, the salt content is 1-8 wt%, and the treatment temperature is 30-40 ℃. The treatment effect is shown in table 2.
TABLE 2 Effect of Paracoccus FSTB-2 on treatment of salt-containing wastewater
As can be seen from Table 2, Paracoccus FSTB-2 can tolerate 1wt% -8wt% of salt, can grow at a high temperature of 40 ℃, and can efficiently remove COD in saline wastewater.
The paracoccus FSTB-2 has very good adaptability and treatment effect on actual salt-containing wastewater, has a strong COD degradation function, can be used for treating the salt-containing wastewater independently, can also be used as an exogenous functional microorganism to be added to the existing process flow after being amplified and cultured independently, can be used for optimizing and adjusting the actual process condition in a targeted manner, and solves the problems of poor treatment effect and easy degradation of activated sludge in a high-salt environment. The method for treating the high-salt-content wastewater by adopting the halotolerant bacteria has lower cost than a physical chemical method, can generate more obvious economic benefit and social benefit when being directly applied, and can provide technical support for the standard-reaching discharge of the high-salt-content related wastewater treatment.
SEQUENCE LISTING
<110> China petrochemical Co., Ltd
Dalian petrochemical research institute of China petrochemical company Limited
<120> Paracoccus and culture application thereof
<160>1
<170>PatentIn version 3.5
<210>1
<211>1295
<212>DNA
<213>Paracoccus sp.
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agccctgggaaactgggagtaataccgcatgagccctacgggggaaagatttatcggcaa 120
aggatcggcccgcgttggattaggtagttggtggggtaatggcctaccaagccgacgatc 180
catagctggtttgagaggatgatcagcccacactgggactgagacacggcccagactcct 240
acgggaggcagcagtggggaatcttagacaatgggggcaaccctgatctagccatgccgc 300
gtgagtgatgaaggccttagggttgtaaagctctttcagctgggaagataatgacggtac 360
cagcagaagaagccccggctaactccgtgccagcagccgcggtaatacggagggggctag 420
cgttgttcggaattactgggcgtaaagcgcacgtaggcggaccagaaagttgggggtgaa 480
atcccggggctcaacctcggaactgccttcaaaactattggtctggagttcgagagaggt 540
gagtggaattccgagtgtagaggtgaaattcgtagatattcggaggaacaccagtggcga 600
aggcggctcactggctcgatactgacgctgaggtgcgaaagcgtggggagcaaacaggat 660
tagataccctggtagtccacgccgtaaacgatgaatgccagtcgtcgggcagcatgctgt 720
tcggtgacacacctaacggattaagcattccgcctggggagtacggtcgcaagattaaaa 780
ctcaaaggaattgacgggggcccgcacaagcggtggagcatgtggtttaattcgaagcaa 840
cgcgcagaaccttaccaacccttgacatcctcggatcggcctggagacaggtcttccact 900
tcggtggccgagtgacaggtgctgcatggctgtcgtcagctcgtgtcgtgagatgttcgg 960
ttaagtccggcaacgagcgcaacccacactttcagttgccatcatttggttgggcactct 1020
ggaagaactgccgatgataagtcggaggaaggtgtggatgacgtcaagtcctcatggccc 1080
ttacgggttgggctacacacgtgctacaatggtggtgacagtgggttaatccccaaaagc 1140
catctcagttcggattggggtctgcaactcgaccccatgaagttggaatcgctagtaatc 1200
gcggaacagcatgccgcggtgaatacgttcccgggccttgtacacaccgcccgtcacacc 1260
atgggagttgggtctacccgacggccgtgcgctaa 1295
Claims (7)
1. Paracoccus FSTB-2, which is classified and named as Paracoccus (A)Paracoccus sp.) The strain is preserved in China general microbiological culture Collection center (CGMCC) at 1/6/2015 with the preservation number of CGMCC No. 10938.
2. Use of paracoccus FSTB-2 according to claim 1 for COD removal from salt-containing wastewater.
3. Use according to claim 2, characterized in that: the strain is applied to the efficient removal of COD in saline wastewater with salt content of 1.0-8.0 wt%, and can grow at a high temperature of 40 ℃.
4. Use according to claim 2, characterized in that: the salt-containing wastewater is chemical synthesis wastewater and coal chemical wastewater which are pretreated, or strong brine generated by the reverse osmosis reduction unit of wastewater generated by an oil refining process, a coal chemical process and a chemical synthesis process, wherein the salt content is 0.5-8.0 wt%, and the COD content is 200-20000 mg/L.
5. The method for culturing Paracoccus FSTB-2 according to claim 1, which comprises three stages of strain activation, liquid seed culture and aeration culture, and comprises the following specific steps:
(1) strain activation: inoculating Paracoccus FSTB-2 to slant or plate of FSTB solid culture medium, culturing at 25-40 deg.C for 24-48 hr, and storing in 4 deg.C refrigerator;
(2) liquid seed liquid culture: preparing an FSTB liquid culture medium, subpackaging in a triangular flask, sterilizing, cooling to room temperature, selecting the activated strain in a slant or a flat plate under an aseptic environment, inoculating into the triangular flask, and culturing at 25-40 ℃ for 24-72 h;
(3) aeration culture: adding an FSTB-2 liquid culture medium into a closed reactor provided with an aeration device, inoculating a liquid seed solution according to the proportion of 0.5-25% of the volume ratio of the reactor, controlling the pH value to be 6.0-8.5, carrying out aeration culture for 48-96 hours, then carrying out periodic feeding and discharging operation, wherein the discharging amount accounts for 5-90% of the volume of the reactor, the feeding amount accounts for 5-90% of the volume of the reactor, the culture period is 1 culture period after 24-48 hours of culture, and then discharging the culture solution with the corresponding volume according to the proportion to obtain a concentrated bacterial liquid product containing high-concentration paracoccus.
6. The method of claim 5, wherein: the FSTB liquid culture medium comprises: FeSO4•7H2O 25mg/L，NH4NO3286mg/L，KCl 929mg/L，CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, and pH 6.0-8.5; the FSTB solid culture medium is prepared by adding 20g/L agar to a liquid culture medium.
7. A salt-tolerant microbial inoculum for removing COD is characterized in that: the microbial inoculum comprises Paracoccus (Paracoccus sp.）FSTB-2。
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| CN111348752A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Treatment method of high-salt degradation-resistant wastewater |
| CN110452858B (en) * | 2019-09-12 | 2021-04-27 | 中国科学院微生物研究所 | Paracoccus B54 and application thereof |
| CN110656071B (en) * | 2019-11-11 | 2022-03-04 | 黄河三角洲京博化工研究院有限公司 | Paracoccus huilkii for efficiently degrading DMF (dimethyl formamide) and application thereof |
| CN112723558B (en) * | 2020-12-16 | 2021-12-14 | 青岛尚德生物技术有限公司 | Application of paracoccus denitrificans in preparation of microbial agent for degrading ammoniacal nitrogen in landfill leachate |
| CN112522152B (en) * | 2020-12-16 | 2021-10-01 | 青岛尚德生物技术有限公司 | Salt-tolerant paracoccus denitrificans GBW-HB1904 and application thereof |
| CN113307447B (en) * | 2021-05-24 | 2022-12-06 | 东华大学 | Zero-valent iron mixed culture denitrification coupling modified charcoal two-stage PRB underground water denitrification method and reactor |
| CN114292789B (en) * | 2021-12-31 | 2022-09-13 | 青岛蔚蓝赛德生物科技有限公司 | Paracoccus with denitrification and dephosphorization functions and application thereof |
| CN114456974B (en) * | 2022-01-14 | 2024-04-09 | 浙江台州秀川科技有限公司 | Paracoccus thiooxidans capable of efficiently degrading DMF and application thereof in DMF-containing wastewater treatment |
| CN114410545B (en) * | 2022-02-23 | 2023-07-28 | 中国科学院微生物研究所 | Paracoccus GN-9 and application thereof |
| CN114940957B (en) * | 2022-05-11 | 2024-01-30 | 江苏科技大学 | Paracoccus ubitus with facultative denitrification synchronous denitrification and dephosphorization performances |
| CN114958689B (en) * | 2022-06-29 | 2023-03-10 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
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