CN111117914B - Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application - Google Patents
Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application Download PDFInfo
- Publication number
- CN111117914B CN111117914B CN201911384764.4A CN201911384764A CN111117914B CN 111117914 B CN111117914 B CN 111117914B CN 201911384764 A CN201911384764 A CN 201911384764A CN 111117914 B CN111117914 B CN 111117914B
- Authority
- CN
- China
- Prior art keywords
- strain
- pseudomonas
- ammonia nitrogen
- salt
- bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Abstract
The invention discloses a salt-tolerant heterotrophic aerobic nitrobacter strain, and provides a culture method of the strain, a bacterial liquid containing the strain and an application mode of the strain for removing ammonia nitrogen in wastewater. The strain is separated from activated sludge. The strain is identified as pseudomonas (Pseudomonas sp.). The strain has a high-efficiency denitrification function in a liquid culture medium which takes glucose as a carbon source and ammonium chloride as a unique nitrogen source, has a removal rate of ammonia nitrogen of more than 85 percent and a removal rate of total nitrogen of 70 percent, provides a good microbial material for biological denitrification and treatment of a high-salt high-ammonia nitrogen polluted water body, and has a good market application prospect.
Description
Technical Field
The invention relates to the technical field of environmental microorganisms, and in particular relates to a salt-tolerant heterotrophic aerobic nitrobacteria strain, a culture method, a bacterial liquid and application.
Background
With the development of industry and agriculture and the improvement of the living standard of people, the discharge amount of the waste water containing the nitrogen compounds is increased sharply, and the waste water becomes a main pollution source of the environment and is paid much attention. In recent years, nitrogen pollution becomes a great hidden danger of surface water environmental safety in China, and a large amount of ammonia nitrogen wastewater is discharged into a water body, so that water eutrophication is caused, water body is black and smelly, the difficulty and cost of water treatment are increased, and even toxic action is generated on people and organisms. The ammonia nitrogen in the wastewater mainly comprises two ammonia nitrogen components, wherein one is formed by ammonia water, and the other is formed by inorganic ammonia, and the other is mainly ammonium sulfate, ammonium chloride and the like. The ammonia nitrogen wastewater mainly comes from wastewater discharged by industries such as chemical industry, metallurgy, chemical fertilizer, coal gas, coking, tanning, monosodium glutamate, meat processing, breeding and the like, landfill leachate and the like. The ammonia nitrogen wastewater also has toxic action on fishes and certain organisms. In addition, when the waste water containing a small amount of ammonia nitrogen is reused in the industry, the waste water has a corrosion effect on certain metals, particularly copper, and can also promote the propagation of microorganisms in water pipelines and water using equipment, form biological scale and block the pipelines and the equipment.
The method for treating ammonia nitrogen wastewater is various, and currently, a chemical precipitation method, a stripping method, a chemical oxidation method, a biological method, a membrane separation method, an ion exchange method, soil irrigation and the like are common. The bioremediation technology mainly comprising microorganisms has great application prospect in denitrification of polluted water bodies due to the advantages of high efficiency and low cost.
The microorganism nitrifies NH under the action of nitrifying bacteria3And NH4+Or an organic nitrogen oxidation to hydroxylamine (NH) in a negative trivalent oxidation state2OH）、NO2- -N and NO3-N. At present, heterotrophic nitrification microorganisms exist in soil, sludge, lake water, deep sea, volcanic estuary and other places, and the distribution area is very wide. These heterotypic nitrifying microorganisms with nitrifying function comprise a variety of microbial species, both eukaryotic and prokaryotic, such as fungi, bacteria, actinomycetes, and even algae. The growth, propagation and metabolic activities of microorganisms are greatly influenced by external environmental conditions, such as temperature, pH and nutrients, and the optimal growth conditions of different types of microorganisms are very different.
Disclosure of Invention
Aiming at the defects in the prior art, the first object of the invention is to provide a salt-tolerant heterotrophic aerobic nitrobacter strain which has high-efficiency denitrification capability.
The second purpose of the invention is to provide a culture method of the salt-tolerant heterotrophic aerobic nitrifying bacterial strain, which has the advantages of efficiently and quickly screening the salt-tolerant heterotrophic aerobic nitrifying bacterial strain and ensuring the stable nitrifying and denitrifying properties of the strain.
The third purpose of the invention is to provide a bacterial liquid prepared from the salt-tolerant heterotrophic aerobic nitrobacteria strain, which is applied to the denitrification treatment of wastewater.
The fourth purpose of the invention is to provide the application of the salt-tolerant heterotrophic aerobic nitrobacteria strain, the strain enables the operation of the wastewater treatment process to be simple, the removal rate of ammonia nitrogen in the wastewater reaches more than 85%, the removal rate of total nitrogen also can reach 70%, and the strain provides a good microbial material for biological denitrification and treatment of high-salt high-ammonia nitrogen polluted water.
In order to achieve the first object, the invention provides the following technical scheme: a salt-tolerant heterotrophic aerobic nitrobacter strain is Pseudomonas sp (Pseudomonas sp), is named HQXH-21, is preserved in China general microbiological culture Collection center (CGMCC), and has the address as follows: the microbial research institute of western road No. 1, china academy of sciences, north jing, chaoyang district, with the preservation number: 19106, preserving for: 12 and 6 in 2019.
Further, a single colony of the strain in an LB culture medium is regular round, wet, yellow, opaque and viscous.
Further, the strain is selected from activated sludge.
Further, the isolation and screening of the strain comprises the following steps:
(1) collecting an activated sludge sample, and inoculating the activated sludge sample to a nitrification culture medium by a dilution and flat plate coating method, wherein NH4 is initially contained in the nitrification culture medium+-N concentration of 100mg/L, incubation at 30 ℃;
(2) placing the strain separated in the step (1) into a culture medium for NH4+N removal test, NH4+The strain with the highest N removal rate is the required strain.
The salt-tolerant heterotrophic aerobic nitrobacteria strain provided by the invention is screened from activated sludge, the sludge contains various microorganisms, generally, the activated sludge at the bottom of high ammonia nitrogen wastewater contains a plurality of microorganisms, the microorganisms are contacted with the high ammonia nitrogen wastewater for a long time, one part of the microorganisms can not survive due to the high ammonia nitrogen severe environment, the other part of the microorganisms can adapt to the high ammonia nitrogen environment gradually and can normally survive under the severe environment, most of the microorganisms are nitrifying bacteria, and the nitrifying bacteria have denitrification capability.
Further, the heterotrophic nitrification capacity of the strain is detected, which comprises the following steps,
(1) preparing a seed solution: inoculating the strain into LB culture medium containing NaCl 10g/L, and culturing at 25-35 deg.C;
(2) and (3) collecting thalli: obtaining thalli in the seed liquid in the step (1) and preparing a thalli suspension;
(3) inoculation: inoculating the thallus heavy suspension obtained in the step (2) to a heterotrophic nitrification culture medium with a unique nitrogen source, and culturing at the temperature of 25-35 ℃;
(4) sampling and measuring: and (3) measuring the ammonia nitrogen removal degree and the accumulation conditions of nitrate nitrogen and nitrite nitrogen of the strain under different culture times.
By adopting the technical scheme, the heterotrophic nitrification capability of the screened nitrifying bacteria is detected to obtainPseudomonas sp. HQXH-21 is a nitrifying strain with high-efficiency denitrification capability, and the strain is used for denitrification treatment of ammonia nitrogen in wastewater.
In order to achieve the second object, the invention provides the following technical scheme: a method for culturing a salt-tolerant heterotrophic aerobic nitrifying bacterial strain comprises the following steps of inoculating the bacterial strain to a nitrifying culture medium according to the inoculation amount of 2% -10%, wherein the culture medium comprises the following components: 0.1g/L-0.5g/L of ammonium chloride, 0.5g/L-1g/L of sodium acetate, 0.02g/L-0.5g/L of disodium hydrogen phosphate, 0.01g/L-0.1g/L of potassium chloride, 5g/L-10g/L of sodium chloride, 0.01g/L-0.05g/L of magnesium sulfate and 1mL of trace element solution; the pH is 6-8;
placing the nitrifying culture medium inoculated with the strain into a constant-temperature shaking incubator with the culture temperature of 20-40 ℃ for culture; replacing fresh heterotrophic nitrification culture medium at intervals of 24-48 h according to volume ratio of 5-20%, culturing for more than 30h, and selecting OD600The bacterial liquid of 0.4-0.8 is bacterial liquid of bacterial strain.
The concentration ratio of each component of the nitrifying medium for culturing the screened strain, the culture temperature of the strain and the culture pH are researched to obtain the optimal culture condition of the strain, so that the subsequent utilization is ensuredRhodococcus erythropolis. HQC-1 is used for carrying out good state of the strain in the denitrification treatment of the wastewater.
In order to realize the third purpose, the invention provides a bacterial liquid prepared by the salt-tolerant heterotrophic aerobic nitrobacteria strain.
Further, the method for preparing the bacterial liquid comprises the following steps of inoculating the bacterial strain into a heterotrophic nitrification culture medium, culturing in a constant-temperature shaking incubator at the temperature of 20-40 ℃ and the pH value of 6-8, replacing the fresh heterotrophic nitrification culture medium every 24-48 h, culturing for more than 30h, and selecting the bacterial liquid with the OD600 of 0.4-0.8 as the bacterial liquid of the bacterial strain.
By adopting the technical scheme, the bacterial liquid of the screened bacterial strains is prepared, and the ammonia nitrogen in the wastewater can be effectively removed by utilizing the bacterial liquid.
In order to achieve the fourth object, the invention provides the following technical solutions: an application of a salt-tolerant heterotrophic aerobic nitrobacter strain is applied to treatment of high ammonia nitrogen wastewater.
Further, the strain is added into the high ammonia nitrogen wastewater according to the inoculation amount of 2-10% (v/v).
The screened strain is applied to ammonia nitrogen treatment of wastewater, the treatment process is optimized, the strain is inoculated into the high ammonia nitrogen wastewater according to the inoculation amount of 2% -10%, the initial ammonia nitrogen concentration of the high ammonia nitrogen wastewater is controlled at 100mg/L, the high ammonia nitrogen wastewater is cultured at 30 ℃ at 150 r/min, the removal conditions of ammonia nitrogen and total nitrogen are detected, the removal rate of the ammonia nitrogen reaches more than 85%, and the removal rate of the total nitrogen can also reach more than 70%.
In conclusion, the invention provides a salt-tolerant heterotrophic aerobic nitrifying bacterial strain with high-efficiency denitrification, and provides a culture method of the bacterial strain and a better application mode of the bacterial strain for removing ammonia nitrogen in wastewater. The strain is identified as pseudomonas (Pseudomonas sp.) HQXH-21, the bacterial strain has ammonia nitrogen eliminating rate over 85% and total nitrogen eliminating rate up to 70% in liquid culture medium with glucose as carbon source and ammonium chloride as only nitrogen source, and the bacterial strain provides one excellent microbe material for biological denitrification and treating high salt and high ammonia nitrogen polluted water bodyAnd (4) market application prospect.
Drawings
FIG. 1 shows a schematic view of the present inventionPseudomonas sp.And (4) detecting the nitration capability of HQXH-21.
FIG. 2 shows a view of the present inventionPseudomonas sp.And (3) detecting the nitration capability of HQXH-21 under different C/N conditions.
FIG. 3 shows a view of the present inventionPseudomonas sp.And (3) detecting the nitration capability of HQXH-21 under different temperature conditions.
FIG. 4 shows a view of the present inventionPseudomonas sp.And (3) detecting the nitration capability of HQXH-21 under different pH conditions.
Detailed Description
The invention provides a salt-tolerant heterotrophic aerobic nitrifying strain, a culture method of the strain and a treatment process for removing ammonia nitrogen content in wastewater. The strain is separated from activated sludge collected by petroleum harbor petrochemical company in Tianjin City. Identifying the strain as Pseudomonas (Pseudomonas) by using 16S r RNAPseudomonas sp.) And is named asPseudomonas sp.HQXH-21, deposited in China general microbiological culture Collection center (CGMCC), with the address: the microbial research institute of western road No. 1, china academy of sciences, north jing, chaoyang district, with the preservation number: 19106, preserving for: 12 and 6 in 2019.
The present invention will be described in further detail with reference to the following drawings and examples.
Example one
The separation, enrichment and screening steps of the heterotrophic nitrification strain are as follows:
(1) activated sludge was collected from the petrochemical company Dagang, China, Tianjin, and sludge samples were inoculated into sterilized nitrification medium by dilution and plate coating methods, starting with NH4 +The N concentration is 100mg/L, and the formula of the nitrification medium is as follows (g/L): 0.3 parts of ammonium chloride, 1 part of sodium acetate, 0.2 parts of disodium hydrogen phosphate, 0.1 part of potassium chloride, 10 parts of sodium chloride, 0.05 part of magnesium sulfate and 1mL of trace element solution, wherein the pH value is 7.0, and the mixture is cultured on a shaking table at the temperature of 30 ℃ to obtain an isolated strain;
(2) placing the strain separated in the step (1)NH in culture Medium4 +N removal test, NH4 +The strain with the highest N removal rate is the required strain.
Example two
Molecular biological characterization of strains
Screening 30 strains from the activated sludge, selecting HQXH-21 strain with the best ammonia nitrogen removal effect to identify the HQXH-21 strain as the pseudomonas (the pseudomonas) by adopting 16S RNAPseudomonas sp.) And is named asPseudomonas sp. HQXH-21, wherein the single colony of the strain in LB culture medium is regular round, moist, yellow, opaque and viscous. The sequence listing in the identification of 16S r RNA of the strain of the invention is shown in the appendix.
EXAMPLE III
Pseudomonas sp. HQXH-21 culture process
The separated bacterial strain is cultured by adopting a nitrifying culture medium, and the composition of the bacterial strain is as follows: 0.3g/L of ammonium chloride, 1g/L of sodium acetate, 0.2g/L of disodium hydrogen phosphate, 0.1g/L of potassium chloride, 10g/L of sodium chloride, 0.05g/L of magnesium sulfate and 1mL of trace element solution; the culture temperature is 30 ℃; the pH is 7;
inoculating the strain to a nitrifying culture medium according to the inoculation amount of 5%, and performing shaking culture at 37 ℃ at 150 r/min; replacing fresh heterotrophic nitrification culture medium every 48h according to the volume ratio of 10%; continuously culturing for more than 30 h.
Example four
Pseudomonas sp. Heterotrophic nitrification capacity detection of HQXH-21
Through the screening and separation of the strains in the activated sludge, the strain with better ammonia nitrogen removal capability is obtainedPseudomonas sp. HQXH-21, the heterotrophic nitrification capacity of the strain is detected, and the specific steps are as follows:
(1) preparing a seed solution: will be provided withPseudomonas sp. HQXH-21 is inoculated in LB culture medium (containing NaCl 10 g/L), and is cultured for 12 h under the conditions of constant temperature and 150 r/min, OD600Is 0.5;
(2) collecting the thallus in the seed liquid prepared in the step (1): centrifuging to remove the seed liquidSeparating the thallus from the fermentation liquid to obtainPseudomonas sp. HQXH-21 bacteria, and suspending with sterile water to prepare bacteria weight suspension;
(3) inoculation: inoculating the thallus heavy suspension of HQXH-21 collected in the step (2) into a heterotrophic nitrification culture medium taking ammonium chloride as a unique nitrogen source, and carrying out shake cultivation at the temperature of 30 ℃ and the rotating speed of 150 r/min;
(4) sampling and measuring: the removal degree of the bacterial strain to ammonia nitrogen and the accumulation condition of nitrate nitrogen and nitrite nitrogen are respectively measured in 2 h, 6 h, 12 h, 24h, 30h, 36 h, 48h and 60 h, and the growth curves are synchronously drawn, as shown in figure 1. As shown in figure 1, the strain has strong ammonia nitrogen removal capability.
In addition, as shown in FIG. 1, the strain has a better growth state in a period of 12-24 h, and the corresponding OD is selected600The bacterial liquid of 0.4-0.8 is bacterial liquid of bacterial strain.
EXAMPLE five
The growth, propagation and metabolic activities of the microorganisms are greatly influenced by external environmental conditions, such as temperature, pH and nutrient substances, and the optimal growth conditions of different types of microorganisms are greatly different, so that the factors influencing the denitrification performance of the strain are further researched, and the research results are further detailed below.
(1) Different C/N pairsPseudomonas sp.Effect of HQXH-21 Denitrification Performance
C/N is an important factor influencing the denitrification reaction of microorganisms, and the research suggests that the rate of the nitrification reaction is reduced due to the fact that the C/N is too high, and the denitrification reaction is inhibited due to the fact that the nitrification reaction is too long due to the fact that the C/N is too low, so that the proper C/N is one of the necessary conditions for the denitrification reaction. In the invention, on the basis of a nitrification culture medium with the sodium chloride concentration of 10g/L and the initial ammonia nitrogen concentration of 100mg/L, the C/N is respectively 2: 1. 4: 1. 6: 1. 8: 1. 10: 1, will be logarithmic in growth phasePseudomonas sp.HQXH-21 is inoculated into 250 mL of nitrifying culture medium with different C/N concentrations according to the inoculation amount of 2-10 percent, cultured in a constant temperature shaking box with the temperature of 30 ℃ and the speed of 150 r/min, and respectively detected under different C/N conditions at different timesPseudomonas sp.Growth and denitrification of HQXH-21As shown in fig. 2. As can be seen from FIG. 2, as the C/N ratio of the nitrified medium increased,Pseudomonas sp.the ammonia nitrogen removal rate of HQXH-21 is obviously improved; when the C/N of the nitrified medium was increased to 10: when the pressure of the mixture is 1, the pressure is lower,Pseudomonas sp.the ammonia nitrogen removal rate of HQXH-21 reaches 90 percent; in the above process, the TOC removal rate is reduced.
(2) Different temperature pairPseudomonas sp.Effect of HQXH-21 Denitrification Performance
Adjusting the C/N to be the optimal C/N obtained in the step (1) and the pH to be 7 on the basis of the nitrification culture medium with the sodium chloride concentration of 10g/L and the initial ammonia nitrogen concentration of 100mg/L, and carrying out logarithmic growth phasePseudomonas sp.Inoculating HQXH-21 into 250 mL of nitrifying culture medium according to the inoculation amount of 2-10%, culturing in a constant-temperature shaking box at the temperature of 25 ℃, 30 ℃ and 35 ℃ at 150 r/min respectively, and detecting NH4+ -N of supernatant and OD in the culture medium after 24h respectively600As a function of time, the temperature of the sample,Pseudomonas sp.the growth and denitrification of HQXH-21 are shown in FIG. 3. As can be seen from fig. 3, as the temperature increases,Pseudomonas sp.the ammonia nitrogen removal rate of HQXH-21 shows an upward trend; when the temperature is set to be 30 c,Pseudomonas sp.the ammonia nitrogen removal rate of HQXH-21 reaches 96 percent; the ammonia nitrogen removal rate of the strain shows a descending trend along with the continuous increase of the temperature.
(3) Effect of different pH on Denitrification Performance of Pseudomonas sp. HQ XH-21
Taking a nitrification culture medium with the sodium chloride concentration of 10g/L and the initial ammonia nitrogen concentration of 100mg/L as the basis, and carrying out logarithmic growth phasePseudomonas sp.Respectively inoculating HQXH-21 into 250 mL of nitrifying culture medium with pH of 5, 6, 7, 8, 9 and 10 according to the inoculation amount of 2-10%, culturing in a constant-temperature shaking box at 30 ℃ and 150 r/min, and respectively detecting NH4+ -N of supernatant and OD in the culture medium after 24h600As a function of time, the temperature of the sample,Pseudomonas sp.the growth and denitrification of HQXH-21 are shown in FIG. 4. As can be seen from FIG. 4, as the pH of the nitrified medium increased,Pseudomonas sp.the ammonia nitrogen removal rate of HQXH-21 is obviously improved; when the pH of the nitrification medium was increased to 7,Pseudomonas sp.the ammonia nitrogen removal rate of HQXH-21 reaches 96.8 percent; followed byThe ammonia nitrogen removal rate of the strain is reduced with the continuous increase of the pH of the nitrification medium.
EXAMPLE six
Pseudomonas sp. Application of HQXH-21 in treatment of high-salt high-ammonia nitrogen wastewater
Aiming at the nitration capability of the strain Pseudomonas sp. HQXH-21, the method is used for treating high-salt high-ammonia nitrogen wastewater. Selecting OD according to the method of example five600Inoculating 0.8 bacterial liquid into high-salt high-ammonia-nitrogen wastewater according to the inoculation amount of 5% (v/v), controlling the initial ammonia-nitrogen concentration of the high-salt high-ammonia-nitrogen wastewater to be about 100mg/L, controlling the pH to be about 7, controlling the salt content to be 60g/L, culturing at 30 ℃ and 150 r/min, detecting the removal condition of ammoniacal nitrogen and total nitrogen after 36 h, wherein the removal rate of the ammoniacal nitrogen is more than 85%, and the removal rate of the total nitrogen can also be more than 70%.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Sequence listing
<110> Beijing Han Qi environmental technology Co., Ltd
<120> salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application
<130> WZF015PTA01F1910132
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 865
<212> DNA
<213> Pseudomonas sp (Pseudomonas sp.)
<400> 1
aaggcccggg aacgtattca ccgcgacatt ctgattcgcg attactagcg attccgactt 60
cacgcagtcg agttgcagac tgcgatccgg actacgatcg gttttgtgag attagctcca 120
cctcgcggct tggcaaccct ctgtaccgac cattgtagca cgtgtgtagc ccaggccgta 180
agggccatga tgacttgacg tcatccccac cttcctccgg tttgtcaccg gcagtctcct 240
tagagtgccc accataacgt gctggtaact aaggacaagg gttgcgctcg ttacgggact 300
taacccaaca tctcacgaca cgagctgacg acagccatgc agcacctgtg tcagagttcc 360
cgaaggcacc aatccatctc tggaaagttc tctgcatgtc aaggcctggt aaggttcttc 420
gcgttgcttc gaattaaacc acatgctcca ccgcttgtgc gggcccccgt caattcattt 480
gagttttaac cttgcggccg tactccccag gcggtcaact taatgcgtta gctgcgccac 540
taaaatctca aggattccaa cggctagttg acatcgttta cggcgtggac taccagggta 600
tctaatcctg tttgctcccc acgctttcgc acctcagtgt cagtatcagt ccaggtggtc 660
gccttcgcca ctggtgttcc ttcctatatc tacgcatttc accgctacac aggaaattcc 720
accaccctct accgtactct agcttgccag ttttggatgc agttcccagg ttgagcccgg 780
ggctttcaca tccaacttaa caaaccacct acgcgcgctt tacgcccagt aattccgatt 840
aacgcttgca ccctctgtat tacgc 865
Claims (6)
1. A salt-tolerant heterotrophic aerobic nitrobacter strain is characterized in that: the strain is pseudomonas (Pseudomonas sp.) And is named asPseudomonas sp.HQXH-21, deposited in China general microbiological culture Collection center (CGMCC), with the address: the microbial research institute of western road No. 1, china academy of sciences, north jing, chaoyang district, with the preservation number: 19106, preserving for: 12 and 6 in 2019.
2. The halotolerant heterotrophic aerobic nitrifying bacterial strain of claim 1 wherein: a single colony of the strain in an LB culture medium is regular round, wet, yellow, opaque and viscous.
3. A bacterial solution prepared by using the salt tolerant heterotrophic aerobic nitrifying bacterial strain of any one of claims 1 to 2.
4. The bacterial liquid according to claim 3, wherein: the method for preparing the bacterial liquid comprises the following steps of inoculating the bacterial strain into a heterotrophic nitrification culture medium, culturing in a constant-temperature shaking incubator at the temperature of 20-40 ℃, controlling the pH to be 6-8, replacing a fresh heterotrophic nitrification culture medium every 24-48 hours, culturing for more than 30 hours, and selecting OD600The bacterial liquid of 0.4-0.8 is bacterial liquid of bacterial strain; the heterotrophic nitrification medium comprises the following components: 0.1g/L-0.5g/L of ammonium chloride, 0.5g/L-1g/L of sodium acetate, 0.02g/L-0.5g/L of disodium hydrogen phosphate, 0.01g/L-0.1g/L of potassium chloride, 5g/L-10g/L of sodium chloride, 0.01g/L-0.05g/L of magnesium sulfate and 1mL of trace element solution; the pH is 6-8.
5. The use of a halotolerant heterotrophic aerobic nitrifying bacterial strain according to any one of claims 1 to 2 wherein: the strain is applied to the treatment of high ammonia nitrogen wastewater.
6. The use of a salt tolerant heterotrophic aerobic nitrifying bacterial strain according to claim 5 wherein: the strain is added into the high ammonia nitrogen wastewater according to the inoculation amount of 2-10% (v/v).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911384764.4A CN111117914B (en) | 2019-12-28 | 2019-12-28 | Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911384764.4A CN111117914B (en) | 2019-12-28 | 2019-12-28 | Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111117914A CN111117914A (en) | 2020-05-08 |
| CN111117914B true CN111117914B (en) | 2021-07-30 |
Family
ID=70504132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911384764.4A Active CN111117914B (en) | 2019-12-28 | 2019-12-28 | Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111117914B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481171A (en) * | 2020-12-14 | 2021-03-12 | 浙江双良商达环保有限公司 | Low-temperature denitrifying bacteria and screening method and application thereof |
| CN112961790B (en) * | 2021-03-29 | 2022-11-01 | 江西沃邦兴环保科技有限公司 | Heterotrophic nitrifying bacteria resistant to high-salt environment and application thereof |
| CN113308393A (en) * | 2021-04-20 | 2021-08-27 | 上田环境修复有限公司 | Preparation for degrading ammonia nitrogen and total nitrogen and preparation method thereof |
| CN114853182B (en) * | 2022-05-07 | 2023-05-02 | 福建工程学院 | Method for treating zinc ion pollution in water based on microbial denitrification |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302485A (en) * | 2008-07-07 | 2008-11-12 | 中国科学院成都生物研究所 | Heterotrophic nitrification microbial preparation, cultivation method and use thereof |
| CN101338282A (en) * | 2007-07-02 | 2009-01-07 | 中国科学院成都生物研究所 | Heterotrophic nitrification aerobic denitrifying bacteria, culturing method thereof and uses |
| CN101705202A (en) * | 2009-12-03 | 2010-05-12 | 华中农业大学 | Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application |
| CN106520624A (en) * | 2016-12-07 | 2017-03-22 | 暨南大学 | Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification |
| CN107201325A (en) * | 2017-05-03 | 2017-09-26 | 上田环境修复股份有限公司 | Pseudomonad strain and its cultural method and application |
| CN108865924A (en) * | 2018-06-05 | 2018-11-23 | 中科美大（北京）生态环境工程有限公司 | Heterotrophic nitrification-biological aerobic denitrification pseudomonad microbial inoculum and preparation method thereof, application |
-
2019
- 2019-12-28 CN CN201911384764.4A patent/CN111117914B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101338282A (en) * | 2007-07-02 | 2009-01-07 | 中国科学院成都生物研究所 | Heterotrophic nitrification aerobic denitrifying bacteria, culturing method thereof and uses |
| CN101302485A (en) * | 2008-07-07 | 2008-11-12 | 中国科学院成都生物研究所 | Heterotrophic nitrification microbial preparation, cultivation method and use thereof |
| CN101705202A (en) * | 2009-12-03 | 2010-05-12 | 华中农业大学 | Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application |
| CN106520624A (en) * | 2016-12-07 | 2017-03-22 | 暨南大学 | Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification |
| CN107201325A (en) * | 2017-05-03 | 2017-09-26 | 上田环境修复股份有限公司 | Pseudomonad strain and its cultural method and application |
| CN108865924A (en) * | 2018-06-05 | 2018-11-23 | 中科美大（北京）生态环境工程有限公司 | Heterotrophic nitrification-biological aerobic denitrification pseudomonad microbial inoculum and preparation method thereof, application |
Non-Patent Citations (2)
| Title |
|---|
| Simultaneous Heterotrophic Nitrification and Aerobic Denitrification by the Marine Origin Bacterium Pseudomonas sp. ADN-42;Ruofei Jin et al.;《Appl Biochem Biotechnol》;20151231;全文 * |
| 耐盐异养硝化菌qy18和中度嗜盐异养硝化菌gs2的脱氮特性与耐盐性研究;曲洋 等;《海洋环境学》;20110630;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111117914A (en) | 2020-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111117914B (en) | Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application | |
| CN107034168B (en) | Heterotrophic nitrification-aerobic denitrification bacterium and application thereof | |
| CN111117912B (en) | Salt-tolerant denitrifying bacteria strain, salt-tolerant denitrifying bacteria liquid, screening method and application | |
| CN107177530B (en) | Novel efficient domestic sewage denitrifying bacterium and application thereof | |
| CN114703095B (en) | Pseudomonas adulthood and application thereof in field of sewage and wastewater purification | |
| CN110656066B (en) | Acinetobacter strain for shortcut nitrification and denitrification and application thereof | |
| CN114890555B (en) | Solid microbial preparation for treating rural black and odorous water body and preparation method and application thereof | |
| Wang et al. | Isolation and characterization of a nitrobenzene-degrading bacterium Klebsiella ornithinolytica NB1 from aerobic granular sludge | |
| CN109337832A (en) | A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application | |
| CN113249273B (en) | Salt-tolerant bacillus subtilis and application thereof in high-salt ammonia nitrogen wastewater treatment | |
| CN114395505A (en) | Low-temperature denitrifying bacterium and application thereof | |
| CN115386520B (en) | Rhodococcus pyridine-philic RL-GZ01 strain and application thereof | |
| CN112266885A (en) | Heterotrophic nitrification aerobic denitrifying bacterium Y16 and application thereof | |
| CN109370931B (en) | Complex microbial inoculant for efficiently degrading polycyclic aromatic hydrocarbon and application thereof | |
| CN108102943B (en) | Denitrifying microorganism and application thereof | |
| CN113583897B (en) | Bacillus aryabhattai FL05 and application thereof | |
| CN110468066B (en) | Aerobic denitrifying strain and application thereof | |
| CN112646742B (en) | Activated sludge culture agent and application thereof | |
| CN115353210A (en) | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater | |
| CN113736700A (en) | Heterotrophic nitrification-aerobic denitrification bacteria and application thereof | |
| CN113337413A (en) | Salt-resistant acid-alkali-resistant heterotrophic nitrifying bacteria and application thereof | |
| CN116555069B (en) | Alkana diesel oil bacterium with salt-tolerant aerobic denitrification characteristic | |
| Xie et al. | Uptake of copper ion by activated sludge and its bacterial community variation analyzed by 16S rDNA | |
| CN113005063B (en) | Pseudomonas putida GY13 and application thereof in sewage treatment | |
| CN115029275B (en) | Chlorobenzene degrading bacterium and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: A310-03, floor 2, block a, No. 9, Shangdi Third Street, Haidian District, Beijing 100085 Patentee after: Beijing Hanqi Environmental Technology Co.,Ltd. Address before: A310-03, floor 2, block a, No. 9, Shangdi Third Street, Haidian District, Beijing 100085 Patentee before: BEIJING HANQI ENVIRONMENT TECHNOLOGY CO.,LTD. |