CN112646742B

CN112646742B - Activated sludge culture agent and application thereof

Info

Publication number: CN112646742B
Application number: CN202011529949.2A
Authority: CN
Inventors: 孟希; 吴定心; 廖东栋; 廖泽远
Original assignee: Wuhan Shuizhiguo Environmental Protection Technology Co ltd
Current assignee: Wuhan Shuizhiguo Environmental Protection Technology Co ltd
Priority date: 2020-12-22
Filing date: 2020-12-22
Publication date: 2022-06-24
Anticipated expiration: 2040-12-22
Also published as: CN112646742A

Abstract

The invention belongs to the technical field of environmental protection, and particularly relates to an activated sludge culture medium and an application thereof, wherein the sludge culture medium is prepared by screening bacillus pumilus (CCTCC NO: M2020550) and bacillus subtilis (CCTCC NO: M2020551) and mixing the bacillus pumilus (CCTCC NO: M2020551) and municipal sludge, so that the problem of treatment of residual sludge in the sewage treatment process is solved, and the bacillus subtilis (CCTCC NO: M2020551) has a detoxifying effect, can be used for assisting nitrifying bacteria, and further improves the quality of treated sewage; the bacillus pumilus (CCTCC NO: M2020550) has extremely strong salt tolerance and can be used for treating wastewater with high salt content.

Description

Activated sludge culture agent and application thereof

Technical Field

The invention belongs to the technical field of environmental protection, and particularly relates to an activated sludge culture medium and application thereof.

Background

Excess sludge is the activated sludge discharged from a secondary sedimentation tank (or a sedimentation area) in an activated sludge system, is a byproduct generated in the sewage treatment process, has huge yield, has toxic and harmful substances in the excess sludge and large treatment pressure, and most sewage plants adopt dehydration treatment on the excess sludge at present, but the water content of the excess sludge is still high, the excess sludge is easy to become black and odorous sludge, generates odor and secondary pollution to the environment.

In some integrated equipment, activated sludge is required to be used, and in some newly-built and already-operated sewage treatment systems, the activated sludge is also required to be used as a carrier of microbial flora, but the acquisition of high-quality activated sludge is troublesome and the acquisition amount is small, and most of the existing methods for acquiring the activated sludge are to ship a muddy water mixture with extremely high water content from some stably-operated sewage systems, so that the acquisition and the transportation are difficult, and many of the existing sewage have the characteristics of high salt and inhibitor, and the microbial flora is difficult to survive and grow in the sewage. Patent CN109250814A discloses a composite activated sludge and a method for sewage treatment, which mainly uses sludge domestication to facilitate the degradation of ammonia nitrogen in sewage systems, but the salt tolerance and inhibition resistance of the composite activated sludge are not mentioned. At present, the research on the utilization of the residual sludge after preservation is few and few, and the excellent activated sludge contains abundant microbial flora, such as some nitrifying bacteria, denitrifying bacteria and some COD degrading bacteria, and patent CN108793407A discloses an acclimatization method of activated sludge for sewage treatment and activated sludge, wherein the method is beneficial to removing COD, TN and TP in the wastewater, and the surface of the cultured activated sludge is coated with bacilli, but the flora involved in the method is single, and whether the method is effective in some wastewater with high salinity and inhibition is still not mentioned.

At present, most of the disclosures are the disposal and conventional resource utilization of activated sludge. For example, patent CN102092829B discloses a process for producing bioflocculant from excess sludge; patent CN106698398A discloses a method for preparing graphene aerogel by using activated sludge; for example, patent CN102167485A discloses an intensified sludge disposal method for activated sludge water treatment process. The invention provides a fermentation production method for producing activated sludge culture medium from excess sludge, which comprises the steps of dehydrating the excess sludge, adding solid microbial inoculum and powder carrier, and adding protective agent to prevent the excess sludge from producing methane by black odor within a certain time, so that the number of beneficial microorganisms in the excess sludge is increased, the problem of difficulty in treating the excess sludge in a sewage plant is solved, high-quality activated sludge is provided for the sewage plant, and the problem of trouble in obtaining sludge in the sewage plant is solved.

Disclosure of Invention

The invention aims to provide an active sludge fungicide, which comprises the main active ingredients of municipal sludge, Bacillus brevis pumilus (CCTCC NO: M2020550) and Bacillus subtilis (CCTCC NO: M2020551).

The invention also aims to provide the application of the activated sludge culture medium in sewage treatment.

In order to achieve the purpose, the invention adopts the following technical measures:

the activated sludge culture agent comprises municipal sludge, Bacillus pumilus SZG-NY-001(CCTCC NO: M2020550) and Bacillus subtilis SZG-JD-001(CCTCC NO: M2020551).

The activated sludge bacteria culture agent comprises the following components in every 100 g: 38.7-45.8g of water, 0.2-3.8g of national BioPower 400Plus of water, and SZG-JD-0011 × 10 of Bacillus subtilis⁶-9×10⁶cfu/g active sludge culture medium, Bacillus pumilus SZG-NY-0010.5X 10⁶-8.5×10⁶cfu/g of activated sludge culture medium, and the balance of municipal sludge;

the activated sludge bacteria culture agent comprises the following components in every 100 g: 38.7-45.8g of water, 15.8-28.8 g of carrier, 0.2-3.8g of national BioPower 400Plus of water, and SZG-JD-0011X 10 of bacillus subtilis⁶-9× 10⁶cfu/g active sludge culture medium, Bacillus pumilus SZG-NY-0010.5X 10⁶-8.5×10⁶cfu/g of activated sludge fungicide and the balance of municipal sludge;

the carrier is as follows: one or more of zeolite powder, benefiting powder, vegetable fat powder, tomato powder or skimmed milk powder.

The protection scope of the invention also includes the application of the activated sludge culture medium in preparing sewage treatment preparations.

Or the application of the activated sludge culture medium as an effective component or one of the effective components in sewage treatment.

In the above-mentioned application, it is preferable to remove ammonia nitrogen, total nitrogen and COD in water at the same time.

Compared with the prior art, the invention has the following advantages:

according to the sludge culture medium, the sludge culture medium prepared by screening bacillus pumilus (CCTCC NO: M2020550) and bacillus subtilis (CCTCC NO: M2020551) and mixing the bacillus pumilus (CCTCC NO: M2020550) and the municipal sludge solves the problem of disposal of excess sludge, delays the problem of methane production due to black odor of the excess sludge, solves the problem of shortage of high-quality activated sludge in sewage plants, and increases the number of viable bacteria in the activated sludge.

Meanwhile, the bacillus subtilis (CCTCC NO: M2020551) has the detoxification effect, can be used for assisting nitrifying bacteria, and further improves the quality of treated sewage; bacillus pumilus (CCTCC NO: M2020550) has strong salt tolerance and can be used for treating wastewater with high salt content.

Detailed Description

The present invention is described in detail with reference to the following embodiments, which are implemented on the premise of the technical solution of the present invention, and the examples are only used for explaining the present invention and are not used for limiting the scope of the present invention. The technical scheme of the invention is a conventional scheme in the field if not specifically stated; the reagents or materials, if not specifically mentioned, are commercially available.

Example 1:

obtaining Bacillus pumilus (Bacillus pumilus) SZG-NY-001:

taking fresh sludge of a salt lake in the North of a lake, diluting and taking 10^-4，10^-5，10^-6Three gradient coats were applied, incubated at 37 ℃ for one day, and single colonies were picked and inoculated onto the slant.

The method comprises the following steps of streak-culturing by using an LB solid culture medium containing 5.0% of sodium chloride salt (namely, 5g of sodium chloride is added into 100g of water), 10.0%, 15.0%, 20.0%, 25.0% and 30.0%, culturing for 3 days at 30 ℃, observing the growth condition, selecting 3 strains with better salt resistance, and carrying out attribution identification on the strains to finally identify a salt-tolerant bacillus pumilus.

The strain is delivered to China center for type culture Collection in 28 months at 2020, 9, and is classified and named as follows: bacillus pumilus SZG-NY-001 with the preservation number of CCTCC NO: M2020550, address: the bacillus pumilus is named as bacillus pumilus 001 and is gram-positive bacteria, aerobically grows, hydrolyzes starch, is positive in contact enzyme reaction, is negative in oxidase and lecithinase reaction, and grows at the temperature of 10-45 ℃; colony characteristics: the strain is in a short rod shape under an optical microscope, and is white, wet, neat, round, small and convex in edge on an agar plate culture medium.

Fermentation culture:

shake flask culture medium: 10g/L peptone, 5g/L yeast extract, 5g/L casein, 2g/L KCl, 3g/L sodium citrate, MgSO₄`7H₂20g/L of O, 80g/L of NaCl, 50mg/L of nysfungin, pH8.0, and the balance of water.

Selecting Bacillus pumilus SZG-NY-001 on the plate with an inoculating loop, inoculating in the culture medium, shake culturing at 30 deg.C and 200rpm/min for 24 hr to obtain liquid microbial inoculum seed liquid with effective bacterial concentration of 1 × 10⁸cfu/mL。

And (3) detecting the salt tolerance of the Bacillus pumilus SZG-NY-001:

inoculating 5% SZG-NY-001 or SZG-NY-002 seed solution of Bacillus pumilus in LB culture medium containing NaCl in concentration of 0%, 12%, 14%, 16%, 18% or 20%, culturing for 24 hr, and counting plates, the results are as follows:

growth of Bacillus brevis 001 in culture media with different NaCl concentrations

Concentration of NaCl	Bacterial concentration (cfu/mL)
		0％	1×10⁸
12％	9×10⁷
		14％	8×10⁷
16％	5×10⁶
		18％	3×10⁵
20％	4×10⁴

The results show that the Bacillus pumilus 001 has no inhibiting effect on the growth of the invention when the NaCl concentration is not higher than 14%; when the NaCl concentration is more than 16%, the growth inhibition effect is obvious, and the bacteria concentration is greatly reduced; when the NaCl concentration is 18%, the bacteria concentration is only 3X 10⁵cfu/m. Thus, it is tolerant up to 16% of the salinity of sodium chloride.

The effect of the bacillus pumilus SZG-NY-001 on treating industrial salt-containing wastewater is as follows:

100ml of industrial saline wastewater is put into a 250ml triangular flask, and the activated Bacillus pumilus (SZG-NY-001 seed liquid (effective bacteria concentration is 10%) is inoculated according to the volume ratio of 1 percent⁸cfu/ml), and is placed at 30 ℃ for 200The culture was carried out at rpm, and the soluble COD was measured every day.

Water quality condition (mg/L) of industrial salt-containing wastewater to be treated

In the above table, the "mixed wastewater of p-hydroxybenzaldehyde and tosyl chloride" is the wastewater of dye and dye intermediate. The p-toluenesulfonamide waste water is the waste water of the dye intermediate.

The chemical wastewater is mixed wastewater of a plasticizer, a rubber foaming agent and a dye intermediate.

COD degradation condition (mg/L) of wastewater to be treated after being put into Bacillus pumius SZG-NY-001

As can be seen from the table above, the Bacillus pumilus SZG-NY-001 can tolerate the wastewater with the industrial salt content of about 16.5%, and the COD reduction effect after the strain feeding is obvious.

Example 2:

obtaining Bacillus subtilis SZG-JD-001;

the applicant occasionally screens a strain from the applicant's own strain library, and the strain not only has the denitrification function, but also has the function of a strain antidote; when the nitrifying bacteria are added into the sewage and the nitrifying function of the nitrifying bacteria cannot be realized due to the toxicity of substances in the sewage, the function of the nitrifying bacteria is recovered after the bacterial strain is added. The sequence sequencing result of a partial variable region of the 16srDNA of the strain is compared by NCBI/blast to find that the strain belongs to the bacillus subtilis.

The strains are delivered to China center for type culture Collection at 28/09/2020, and are classified and named: bacillus subtilis SZG-JD-001 with the preservation number of CCTCC NO: M2020551, address: wuhan university in Wuhan, China.

The colony morphology of the Bacillus subtilis SZG-JD-001 is as follows: the bacterial colony is pure white and round, and has wrinkles on the surface, non-transparency and neat edges.

Culturing the Bacillus subtilis SZG-JD-001:

inoculating a strain glycerol tube preserved at the temperature of minus 80 ℃ into an LB liquid culture medium, performing activated culture at the temperature of 30 ℃ for 24h at 200r/min, and then transferring the strain glycerol tube into a fresh LB liquid culture medium for culture for 12h by using the inoculum size of 1%, wherein the number of bacteria is 8.5 hundred million CFU/mL.

The characteristics of the Bacillus subtilis SZG-JD-001:

1) salt tolerance

Respectively inoculating the activated Bacillus subtilis SZG-JD-001 bacterial solution into LB culture media with the total NaCl content of 1 percent, 2 percent (namely adding 2g of sodium chloride in 100g of LB culture media), 4 percent, 6 percent, 8 percent, 10 percent and 12 percent according to the inoculation amount of 1 percent, culturing for 24 hours at 30 ℃ and 200r/min, and detecting the light absorption value of each group of bacterial solution under 600nm, wherein the results are as follows:

growth conditions of Bacillus subtilis SZG-JD-001 in LB culture media with different NaCl concentrations

NaCl concentration%	OD₆₀₀
		1	1.561
2	1.574
		4	1.608
6	1.270
		8	0.167
10	Hardly grown for 24h, OD 48h₆₀₀1.320
		12	Does not grow

The result shows that the Bacillus subtilis SZG-JD-001 grows well in an LB culture medium containing 1-6% NaCl, when the salt concentration reaches 8% and 10%, the strain can generate a delay period in growth, and after the salt concentration exceeds 10%, the strain cannot grow, so that the strain has better salt resistance.

2) Sugar resistance characteristics

Respectively inoculating the activated Bacillus subtilis SZG-JD-001 bacterial solution into LB culture media with the total glucose content of 0 percent, 10 percent (namely adding 10g of glucose in 100g of LB culture media), 20 percent, 30 percent, 40 percent, 50 percent and 60 percent of glucose according to the inoculation amount of 1 percent, culturing for 24 hours at 30 ℃ and 200r/min, and detecting the light absorption value of each group of bacterial solution under 600nm, wherein the results are as follows:

the results show that as the sugar concentration increases, the growth of Bacillus subtilis SZG-JD-001 gradually worsens, with a maximum tolerance of 30% of the glucose concentration.

Stability of the biological composite carbon source prepared from Bacillus subtilis SZG-JD-001:

the biological composite carbon source comprises the following components in every 100 g: 60g of sodium acetate solution, 40g of glycerol solution, 100 hundred million CFU of Bacillus subtilis SZG-JD-001 (added in a bacterial powder form, and the mass is ignored); wherein the mass fraction of the sodium acetate solution is 30 percent, and the mass fraction of the glycerol solution is 85 percent. The preservation effect of the biological composite carbon source at room temperature is detected:

the number of the Bacillus subtilis SZG-JD-001 bacteria is slightly reduced after the biological composite carbon source compounded by the sodium acetate and the glycerol is stored at room temperature for 30 days.

3) Denitrification function

The denitrification function of the strain is detected by adopting a denitrification verification culture medium, and the formula of the culture medium is as follows: 20g/L of peptone, 3g/L of beef extract, 5g/L of sodium chloride and 1g/L of potassium nitrate, adjusting the pH value to 7.4 +/-0.2, subpackaging test tubes and sterilizing at 121 ℃ for 30 min. The activated Bacillus subtilis SZG-JD-001 bacterial liquid is subjected to gradient dilution and coated on an LB solid plate, and then the liquid is inverted into an incubator at 30 ℃ for cultivation for 24 hours. After single bacteria grow on the plate, randomly selecting 5-10 bacterial colonies, inoculating the bacterial colonies in a denitrification verification culture medium, culturing for 72h at 37 ℃, and dropwise adding 1-2 drops of diphenylamine reagent into the test tube during detection to show that the Bacillus subtilis SZG-JD-001 has denitrification capability.

4) Detoxification function

A detoxification function experiment of Bacillus subtilis SZG-JD-001 is carried out by adopting simulated wastewater, and the experimental design is as follows:

experimental scheme for simulating wastewater by using aniline

Simulation of scrapThe water is prepared by adopting a nitrobacteria culture medium and aniline with different concentrations. Wherein the concentration of each component of the nitrobacteria culture medium is 0.5g/L (NH)₄)₂SO₄、0.3g/L NaCl、0.03g/L FeSO₄·7H₂O、0.03g/L MgSO₄·7H₂O、 0.256g/L Na₂HPO₄、3.27g/LK₂HPO₄The aniline addition concentration is 30mg/L and 50mg/L, and the balance is water.

The effective bacterial concentration of the nitrifying bacteria agent is 4 hundred million cfu/ml, and the nitrifying bacteria agent is prepared by using European Nitrosomonas (Nitrosomonas europaea) ATCC19178 and Nitrobacter Winogradskyi (Nitrobacter Winogradskyi) Y3-2(CN105274029B) according to the effective bacterial concentration of 1: 1.

The results of the experiments are shown in the following table:

ammonia nitrogen concentration change (mg/L) during the experiment

The result shows that the nitrifying bacteria agent reacts for 72 hours without additionally adding aniline, and the degradation rate of the nitrifying bacteria agent to ammonia nitrogen is 97.68%; when the concentration of aniline is 30mg/L, the reaction is carried out for 72h, the ammonia nitrogen added with detoxification bacteria (experiment group 1) is reduced from 100.72mg/L to 22.34mg/L, the removal rate is 77.82%, and the removal rate is improved by 73.76% compared with that of a control group (CK 1). When the concentration of aniline is 50mg/L, the degradation time is 72h, the ammonia nitrogen of the experimental group (experimental group 2) added with the detoxification bacteria is reduced from 109.32mg/L to 67.87mg/L, the removal rate is 37.92 percent, and the ammonia nitrogen of the control group (CK2) is slightly increased. This shows that Bacillus subtilis SZG-JD-001 can be used for degrading the ammonia nitrogen concentration in wastewater by cooperating with nitrobacteria when treating aniline-containing wastewater, and has good degradation effect.

Example 3:

an activated sludge culture agent:

every 100g of the activated sludge bacteria culture agent comprises: 42g of water, 23g of zeolite powder, 2g of national BioPower 400Plus of water, 7.8X 10 of Bacillus subtilis SZG-JD-001(CCTCC NO: M2020551)⁶cfu/g activated sludge culture medium, Bacillus pumilus SZG-NY-001 (CC)TCC NO:M 2020550)5.5×10⁶cfu/g activated sludge bacteria culture agent, and the balance of municipal sludge. In order to examine the stability and the commercialization characteristic of the culture medium, another two groups of samples are set for carrying out a control test:

fresh sludge: fresh municipal sludge with water content of 87%;

activated sludge CK without addition of other components: centrifuging fresh municipal sludge, and adjusting the fresh municipal sludge to active sludge with the water content of 42%; municipal sludge in the 3 experimental groups is sludge sampled at the same time and the same place.

The samples of the 3 experimental groups are subpackaged by using sealing bags, and the gas production condition, the appearance change condition and the bacteria number change are observed by sampling periodically.

The viable bacteria counting method comprises weighing 10g of each treated sludge by using an analytical balance, placing in a triangular flask, adding 90mL of double distilled water, adding quantitative glass beads, placing in a shaking table at 37 ℃ and 200r/min, and shaking for 10min to serve as 10^-1Gradient standby; each sample was diluted to 10^-2、10^-3、10^-4And 10^-5Gradient, aspirate 100. mu.L of 10^-3And 10^-4Gradient solutions were spread evenly on poured LB medium using a spreading bar, 10^-5Gradient coating 200 mu L; placing the coated flat plate in a constant-temperature incubator for 24h, and counting;

evolution of gas from each sample with time

Experimental group	0d	15d	30d	60d
					Fresh sludge (water content 87%)	Does not produce gas	Gas production	Severe gas evolution	Severe gas evolution
Activated sludge CK without adding other components	Does not produce gas	Does not produce gas	Gas production	Severe gas evolution
					Activated sludge culture agent	Does not produce gas	Does not produce gas	Does not produce gas	Does not produce gas

Change of appearance of each sample with time

Change in viable count of each sample with time (cfu/g)

Experimental group	0d	15d	30d	60d
					Fresh sludge (water content 87%)	1333.3 ten thousand	987.6 Wan	435 ten thousand	73 ten thousand
Activated sludge CK without adding other components	633.3 ten thousand	577 ten thousand	251.9 ten thousand	108 ten thousand
					Activated sludge culture agent	780 ten thousand	654.2 ten thousand	618 ten thousand	473 ten thousand

According to experimental results, the initial number of bacteria of the fresh sludge is high, a certain loss exists in the initial number of bacteria after the fresh sludge is dehydrated, the number of bacteria in the dehydrated activated sludge can be increased after the bacillus pumilus SZG-NY-001 and the bacillus subtilis SZG-JD-001 are additionally added, and the stability of the activated sludge culture agent is optimal from the aspects of appearance, gas production and viable bacteria attenuation along with the prolonging of the preservation time.

Example 4:

the application of the activated sludge culture agent prepared in the example 3 in the pharmaceutical wastewater,

in this embodiment, the pharmaceutical wastewater to be treated has the following specific indexes:

	ammonia nitrogen mg/kg	Nitrite mg/kg	Nitrate mg/kg	Total nitrogen mg/kg	COD mg/kg
						Pharmaceutical sewage	46.36	1.03	1.66	58.4	127.9

The pharmaceutical wastewater contains substances which inhibit the action of nitrifying bacteria.

The experimental grouping conditions are shown in the following table, wherein a treating agent with the sewage volume of 20% is added into each group, the pH value of the sewage is adjusted to 7.5, and sampling is carried out every day to detect the concentration of ammonia nitrogen, total nitrogen and COD:

grouping	Treatment of
		Blank control	Municipal sludge and pharmaceutical wastewater with water content of 87%
Experimental group 1	Municipal sludge and pharmaceutical wastewater with water content of 42%
		Experimental group 2	Activated sludge bacteria culture agent and pharmaceutical wastewater

The results are as follows:

grouping	Total nitrogen degradation rate for 24 h%	48h total nitrogen degradation rate%
			Blank control	33.22	50.78
Experimental group 1	27.9	36.97
			Experimental group 2	64.75	83

Grouping	24hCOD degradation rate%	48hCOD degradation rate%
			Blank control	12.39	12.88
Experimental group 1	11.67	9.78
			Experimental group 2	32.41	47

Ammonia nitrogen degradation rate%

Total nitrogen degradation rate% (initial total nitrogen value-measured value)/initial total nitrogen value × 100

COD degradation rate% (initial COD value-measured value)/initial COD value × 100

The result shows that the degradation rate of the sewage treated by the activated sludge culture agent is far higher than that of the sewage treated by the municipal sludge with different water contents. In the pharmaceutical wastewater, the ammonia nitrogen, total nitrogen and COD degradation effects of the municipal sludge blank control group with the water content of 87 percent are better than the treatment effect of the experiment group 1 added with 42 percent of municipal sludge and worse than the treatment effect of the experiment group 2 added with the activated sludge fungicide, because the viable count contained in 87 percent of fresh sludge is higher than 42 percent of municipal sludge, 42 percent of municipal sludge can cause death of a part of thalli in the dehydration process, and a large amount of beneficial flora is added into the activated sludge fungicide, so the tolerance and the degradation rate in a sewage treatment system are greatly improved.

Example 5:

the application of the activated sludge culture agent prepared in the embodiment 3 in the printing and dyeing wastewater is as follows:

the specific indexes of the printing and dyeing wastewater to be treated are as follows:

	ammonia nitrogen mg/kg	Total phosphorus mg/kg	Total nitrogen mg/kg	COD mg/kg	Salinity of mg/kg
						Printing and dyeing wastewater	36.8	0.2	47.7	252.4	168

The printing and dyeing wastewater also contains aniline substances and different heavy metals: chromium, copper, lead and arsenic.

Experimental grouping conditions are shown in the following table, a treating agent with the volume of 20% of the sewage is added into each group, the pH of the sewage is adjusted to 7.5, and sampling is carried out every day to detect ammonia nitrogen, total nitrogen and COD;

the results are as follows:

grouping	Ammonia nitrogen degradation rate for 24 hours%	48h ammonia nitrogen degradation rate%	Ammonia nitrogen degradation rate of 72h	Ammonia nitrogen degradation rate of 96h
					Blank control	12.22	16.78	15.77	16.1
Experimental group 1	1.89	1.09	1.45	1.48
					Experimental group 2	43.41	68.7	77.2	74.89

Grouping	Total nitrogen degradation rate for 24 h%	48h total nitrogen degradation rate%	Total nitrogen degradation rate of 72h	Total nitrogen degradation rate of 96h
					Blank control	2.36	11.22	45.4	46.78
Experimental group 1	1.41	7.98	36.89	40.7
					Experimental group 2	46.34	51.2	64.5	68.08

Grouping	24hCOD degradation rate%	48hCOD degradation rate%	72hCOD degradation rate	96hCOD degradation rate
					Blank control	13.6	12.11	13.89	10.77
Experimental group 1	10.44	12.58	9.78	5.99
					Experimental group 2	19.87	22.4	38.7	34.21

The printing and dyeing wastewater contains different heavy metals: chromium, copper, lead and arsenic, and aniline substances, wherein when biochemical reaction is directly carried out without pretreatment by other treatment means, the toxic and harmful substances can greatly inhibit nitration reaction and denitrification reaction, 87% of the fresh sludge contains microorganisms with the quantity and activity superior to 42% of the activated sludge, so that in the wastewater treatment process, 87% of ammonia nitrogen, total nitrogen and COD in the fresh sludge blank control group are added to the experimental group 1 with the water content superior to 42%, SZG-NY-001 strain and SZG-JD-001 strain are added to the activated sludge culture medium, the quantity of microorganism flora is increased on the basis of 42% of the activated sludge and the fresh activated sludge, the tolerance and impact resistance in a sewage system are improved, and therefore in the wastewater treatment process, the ammonia nitrogen, the nitrogen and the COD in the experimental group 2 cultured by the activated sludge are added to the experimental group, The degradation effect of total nitrogen and COD is better than that of the experimental group 1 with the water content of 42 percent and fresh sludge with the water content of 87 percent. The adaptability and treatment effect of the activated sludge culture medium disclosed by the invention on waste water are obviously better than those of fresh sludge and activated sludge without other components, and the ammonia nitrogen treatment efficiency and the ammonia nitrogen treatment rate in some inhibitory pharmaceutical waste water and some printing and dyeing waste water with higher salt content are better than those of fresh sludge and activated sludge without other components.

Claims

1. An activated sludge culture agent comprises municipal sludge, Bacillus pumilus (Bacillus pumilus) and Bacillus subtilis (Bacillus subtilis); the preservation number of the bacillus pumilus is CCTCC NO: M2020550, and the preservation number of the bacillus subtilis is CCTCC NO: M2020551.

2. The use of the activated sludge fungicide of claim 1 in the preparation of a sewage treatment formulation.

3. The use of the activated sludge inoculum according to claim 1 for the simultaneous removal of ammonia nitrogen, total nitrogen and COD from water.

4. The use of the activated sludge inoculant of claim 1 in sewage treatment.

5. The use of the activated sludge culture medium of claim 1 in the preparation of a sewage treatment agent in sewage treatment.